CSPI03G23250 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G23250
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytochrome P450
LocationChr3: 20046342 .. 20055083 (-)
RNA-Seq ExpressionCSPI03G23250
SyntenyCSPI03G23250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATACCCAAATTGTTTTAAAAAAAACCCAATTAACCACACCAATAAATAGTGATGACCTGAAAAAATCAAGACACATCACTTCTCTTTTTCTCTCTCTCAAAATTTGAACCAGCTGCAAAATGATCACGGTCATAATCCTTCTTGTCCTCACCATATTTCTTTTCCACAGATTTAGAACAAGGTTATCGTCCTCTGCCGCGAAAAACCTTCCTCCGGGGGCACTTGGGGTCCCATTTATAGGGCAAAGCCTGAGTCTTCTTGGAGCCATGAGAACAAACACAGCAGAGAAATGGCTGCAGAAGAGAGTGGACAAGTACGGCCCAGTTTCAAAGATGACCCTGTTTGGGAAACCGACGGTGTTTATACATGGGGCGGCAGCAAACAAGGCAGTCGTGTTTTCGGGGGAGGAGGGGACGGTGTCGAATAGGCAGGTCGAGTCGTTGAAGATGATTTTAGGGAAAAGGAATTTGACAGAGCTGAGTGGTGAGGATCATAAGAGAGTTAGAGGGGCGTTGGTTTGGTTCTTAAGGCCTCAGACTCTGAGAAGTTATGTGGGGAAAATGGATGGAGAGGTTAGGAGACATTTGAACATGTATTGGCATGGCAACAACGAAGTCACTGTGAGTTCTCAACCAAATCTTTTTTCTTTTTGAAAAATTTGTTACATGTATTATCAAGCCAACAAACAAGATTTTGGTTTTTAGATTTTGAAAATAATTAAATAAATGTTTGGTAACTGTTTTTTGCTTTCTTTTTAAATAAAAAAGACTAAAAAATAAAAACATGTTTGCCCGACTGTTTTCAACAAATTACGAAAAAAAAACAAGAGGTTTTTGTTGAACTTGAGTCAAAGTTCAATCACTTTCTAGTTCTTCGTAACCATTATTAACAATGTTTCACACATCTTATGAACCAAGTGTTGGAGGCGTTGACGAGCCCACTCTAAATTCACTCTAAATCTCACAAACTTTTAAGAGAAGAAATTTGTTCTCTTAGAAAACTCAATAACACACAAGAGAAGAATGTTGTTATCTTGAAACTTACTTACTTTCTAAAACTCAATTTGCTTCTATTGATTTGATTCTTTTATCTTGTATACAAATGAAGGAAATGATCTCTATATATAGTATTCAAGAGACACTTCCTAAAAGACACAAAATAAATGAAGTACATGAATACATACCATTCATGTACTTGAATAATTACATGTATCTAGAAATGCATATGTATCTTGAAACTAGAAATGTATCTATTAATGTGTTATCCATAATGTATCTAGCTTATTAATTTCTAACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATACAAATGAAGGAAATGATCTCTATATATAGTATTCAAGAGACACTTCCTAAAAGACACAAAATAAATGAAGTACATGAATACATACCATTCATGTACTTGAATAATTACATGTATCTAGAAATGCATATGTATCTTGAAACTAGAAATGTATCTATTAATGTGTTATCCATAATGTATCTAGCTTATTAATTTCTAACACCAAGAAAGCTAGAGCTTTCATTCGAATACACCAATTGCTACAACTTTCCTTTGTAAGTAGTACATGGAAGGGAACTAAATTTTTGTTACACCAATTGCTACAACTTTCCTTTGTAAGTTGAAGTACATGGAAGGGAACTAAATTTTTGTTTTCCATTTGCAAAAACAAGTTTTTAAACTCCAAACTCAAACTCTAATAATACTTTGTCGGAAGCCTATTATGAACACATGAACCCCCAAATAAATAATCACAAAATAGAATATTTGAGATAAGAAATAAAAAACTTATTTGAAAGACTTCAACTCTCCTTCATGTGTATTCTTCTTCACACTCAGATACTTTACAATACAATATATCAATCCTATTTATAGGATTGTCATGAGCACAACTCACACCAGAGTTAAACACACAACAATTTAAATCCTACACTTAGTTCATTAACGCGAAACTCATACTAAATTAACTCTAACATTTTAACTTACATTTCTTATTTTTTTATTTTTATTTTTTGCTAACACTCATAAATCACTTCACATCTAACTTATTTAATTAAAACATGTTTAATTTTCAACAAAGTCTGCTTCCCTCCAGTCTAGTCCTAACCTTTTTTGATTTGATACATGGTCTTAACATTTTACATTTTTTTATTTTATTTTATTTTTTTGCCAAAAACACAGGTGGCACCATTGATGAAAACTCTAACCTTTGACATTATATGCTCTCTCCTCTTTGGAATTGAAGAAGGAACAACAAGAAAGAGCATAGTAGAATGCTTCAAAACAATGGTTGATGGCATTTGGTCTATTCCAATAAATCTCCCTTTCACAAGGTATAACCACAGCCTGAAAGCAAGTGCAAAAGCTCAACAGATACTGAAGCAACTTTTGAAAGACAAAGCAACAGTAATGGAAGAAGAAAAAGAAAGTGAAGTAGAAGAAAATGATAAAGACCTGATTAGTTATTTGCTTAGAATAAAAAACAAAGACAAGGAACAAGCTTTGAGTGAGGAGGAAATTGTGCATAATATCATCCTTCTAATGATTGCTGGACATGACACAACTTCTATTTTGCTTACTCTAATGCTTAGGGTTTTGGCTACAAACCCAACTGTTTATGCAGCTGTTCTTCAAGGTATGCATATTTCCGTAAGTTTCTATTTCTATCTTAATGTGTGGGTGTAGATCAACTAATATTGGACGATTTCGAAAAAACATAAGATTTTTTTTGTTAGAAATTGGGATGGTGTTTAAGAATGTAGTGTTTAAGTGAGTTATATTATTATCGTGAATGTGTCTTTACATATAAATTCCATAAAAAAATATTGAAATGAGTCGGATTCGTCGTTAGAAAGTTAAAACTACTCATATTTGTGCGAATCAAAAGTGAATTTAGTTTTAATAGTAACTGACGACATGAATTACATGAACTCTCTCATTTGAAGTTAGTGTTTTTCATCTAGAGTAGTTGTTGCAAACTAAAGTGAGAAGTCATCCATCTCAAGCCGATAGTAATAAACTTTATATAAAATCTCATTTGTTATGGGTTTTAGAGCATGAAGAAATAGGTAGAAGCAAAGAAAGAGGGGAGGCACTGACTTGGGAAGATATTTCAAAGATGAAGTACACATGGAGAGTAGCAATGGAAACCTTGAGACTTTATCCTCCAGTTTTTGGAGGCTTTAGAGTGGCCCTTAAAGACATTCAATTGGGTGCCTACACAATTCCAAAAGGATGGCAAGTAAGCATATTAAATTAACTTCACACCCTTTAGTTTCTTTCCTTTTACTATATAAACTATCATATATATGCAGTTTATTTTGAGTTCCATTTTTTAAAATGGCATATTTAGTTCTTCAAGATCTTTGCAATACAAAATTTCATTCCACATTATAGTTTTTTCCTTTTTTTCAAGTAGTCTTTAAAATTCTCTCAGTGTCTATCTAGTACAAATTAGAAAATGAATATAATTTATAGCACAAGAGAACAATATTTACAAAAATGACAACTTTTTATCGGACATATTTAAAAAATTTTAAAATGTCAATTTTGTCCTTCAGTTTCCTTTTTCTTATGCTATAGTGAGACTTCTATCAATGACATCATGTTTAATATATCAATATATATTTTTCATGAATTTGTTACTTACTGATAACTTAAATTTTGAATTTTAAATGATATAAATGTTGATAGCATAACCTAAAATCAATAATTTATTTCAATAAAAGTTGCAACTATGTCAATTTTATATAGATTCTATTTGTGATAGAAGTTGTCATATTGATAGTATGTTATTGTGATTCAAAGTTATAACCAACAGTATGCTAATCCAATCTCTCTTCTTTGTCTATTTAGGCTAAAAATAGCCACAATATCACTTTAGAGTAGCATTACATTGATACCATTGAAATATAATAACCTATTAGGAATACTGCTTATAGATGCTACCACTGATATCATTTTGTTGCTGAGTACTTGTAGATATATTCATTGATAGTAGTTAATTGTCAAAAATATCAATTGGTAGTCTAACTAAACCTTGTCAATTCAAAATTGATATGTTTACCAAGGAGATATCACTTATAAAAATTACTCTTCTAATACAAACATTGATAGATAATGTTAGTGATATCATTTTAAGTTGATAGCAATATAGAAAAATGACTTATAATAGCTATTAGAAATATCAATTGATAGCAACATTGTTGATCGAAGTGATAGCAACTGATATCACATTTCTTAAATTGAAAGCTATTACTGATGATAGTTATCAGCAACATATAGCAGCTAACCATAGCAACTGATACCATATTTCTCAAATTGAAAGTTTTACTCTACCCTTTAGTGACTAGCAATTATATGATCATATGATAACAAATTTATCAAATAAAAAGCTATTAGTGATATAACATCTATCAGTGCTAGCAACGTATAACAGTTATTAATAGTTATCAATAATAACATTCAATTTAAGAAAAAAGAAAAAAAAAATATTCGTAATGATGACAAGCTATAACTGATAGTAGCCATCAATGATAACAACCAACAACAGCTATTTGTGATATTCACTAATTGTAGCTATTAGTGATAGCTTTTGATGTGAAAAACTATTAGTGAAACGTTAGTATATTAGTCTTCTATTAATTCTTTTGTGCCATGTATCCCATTGTTTTATCATTGTACAGTATATGCTATTATTTGAAGGTTTTTTTTTTTTTTTTTAAGTAACCCTATAGTTTAAAACATGACTAATAACATATCTCTCCATCTTTTAATTTACTATTCAACTGTTCTTTAAAAATGTAAAAAGCATATATTAATTAATAAGTCCTGAAACATTTTAGTTTATGATCACCAATAGGTTAGTTCTCGAATTTTCAAATTTTGTGCTTAAATATATAGGTAATTTAAACTAATATTAATGTGTTATGAATGCATAATTCAGATCTTTTGGGCAGCACCAATGACACACTTGGATGAGACAATATTTGGAGACCCACAAAAGTTTGAACCAAGTAGATTTGATCAAAACCAAACACCAATTCCACCATTTTCTTTCATTGCTTTTGGTGGAGGACCTAGAATCTGCCCTGGCTATGAATTTGCAAAGCTTGAAACACTTGTTACTATCCATTACCTAATCACTCAATTCACTTGGAACCTCTCCTGTTCACAAGATTTCTTGACTAGAGATCCAACTTTGATGCCTAACAAAGGCCTCCCCATCCAAATTTTTCCCAAATCATAAAAACCTCAATCACATATATATGTCGTATCAACTCTCATATTAATGTAATGGAAATTTGTTAGACTCTCGAAATGC

mRNA sequence

ATACCCAAATTGTTTTAAAAAAAACCCAATTAACCACACCAATAAATAGTGATGACCTGAAAAAATCAAGACACATCACTTCTCTTTTTCTCTCTCTCAAAATTTGAACCAGCTGCAAAATGATCACGGTCATAATCCTTCTTGTCCTCACCATATTTCTTTTCCACAGATTTAGAACAAGGTTATCGTCCTCTGCCGCGAAAAACCTTCCTCCGGGGGCACTTGGGGTCCCATTTATAGGGCAAAGCCTGAGTCTTCTTGGAGCCATGAGAACAAACACAGCAGAGAAATGGCTGCAGAAGAGAGTGGACAAGTACGGCCCAGTTTCAAAGATGACCCTGTTTGGGAAACCGACGGTGTTTATACATGGGGCGGCAGCAAACAAGGCAGTCGTGTTTTCGGGGGAGGAGGGGACGGTGTCGAATAGGCAGGTCGAGTCGTTGAAGATGATTTTAGGGAAAAGGAATTTGACAGAGCTGAGTGGTGAGGATCATAAGAGAGTTAGAGGGGCGTTGGTTTGGTTCTTAAGGCCTCAGACTCTGAGAAGTTATGTGGGGAAAATGGATGGAGAGGTTAGGAGACATTTGAACATGTATTGGCATGGCAACAACGAAGTCACTGTGGCACCATTGATGAAAACTCTAACCTTTGACATTATATGCTCTCTCCTCTTTGGAATTGAAGAAGGAACAACAAGAAAGAGCATAGTAGAATGCTTCAAAACAATGGTTGATGGCATTTGGTCTATTCCAATAAATCTCCCTTTCACAAGGTATAACCACAGCCTGAAAGCAAGTGCAAAAGCTCAACAGATACTGAAGCAACTTTTGAAAGACAAAGCAACAGTAATGGAAGAAGAAAAAGAAAGTGAAGTAGAAGAAAATGATAAAGACCTGATTAGTTATTTGCTTAGAATAAAAAACAAAGACAAGGAACAAGCTTTGAGTGAGGAGGAAATTGTGCATAATATCATCCTTCTAATGATTGCTGGACATGACACAACTTCTATTTTGCTTACTCTAATGCTTAGGGTTTTGGCTACAAACCCAACTGTTTATGCAGCTGTTCTTCAAGAGCATGAAGAAATAGGTAGAAGCAAAGAAAGAGGGGAGGCACTGACTTGGGAAGATATTTCAAAGATGAAGTACACATGGAGAGTAGCAATGGAAACCTTGAGACTTTATCCTCCAGTTTTTGGAGGCTTTAGAGTGGCCCTTAAAGACATTCAATTGGGTGCCTACACAATTCCAAAAGGATGGCAAATCTTTTGGGCAGCACCAATGACACACTTGGATGAGACAATATTTGGAGACCCACAAAAGTTTGAACCAAGTAGATTTGATCAAAACCAAACACCAATTCCACCATTTTCTTTCATTGCTTTTGGTGGAGGACCTAGAATCTGCCCTGGCTATGAATTTGCAAAGCTTGAAACACTTGTTACTATCCATTACCTAATCACTCAATTCACTTGGAACCTCTCCTGTTCACAAGATTTCTTGACTAGAGATCCAACTTTGATGCCTAACAAAGGCCTCCCCATCCAAATTTTTCCCAAATCATAAAAACCTCAATCACATATATATGTCGTATCAACTCTCATATTAATGTAATGGAAATTTGTTAGACTCTCGAAATGC

Coding sequence (CDS)

ATGATCACGGTCATAATCCTTCTTGTCCTCACCATATTTCTTTTCCACAGATTTAGAACAAGGTTATCGTCCTCTGCCGCGAAAAACCTTCCTCCGGGGGCACTTGGGGTCCCATTTATAGGGCAAAGCCTGAGTCTTCTTGGAGCCATGAGAACAAACACAGCAGAGAAATGGCTGCAGAAGAGAGTGGACAAGTACGGCCCAGTTTCAAAGATGACCCTGTTTGGGAAACCGACGGTGTTTATACATGGGGCGGCAGCAAACAAGGCAGTCGTGTTTTCGGGGGAGGAGGGGACGGTGTCGAATAGGCAGGTCGAGTCGTTGAAGATGATTTTAGGGAAAAGGAATTTGACAGAGCTGAGTGGTGAGGATCATAAGAGAGTTAGAGGGGCGTTGGTTTGGTTCTTAAGGCCTCAGACTCTGAGAAGTTATGTGGGGAAAATGGATGGAGAGGTTAGGAGACATTTGAACATGTATTGGCATGGCAACAACGAAGTCACTGTGGCACCATTGATGAAAACTCTAACCTTTGACATTATATGCTCTCTCCTCTTTGGAATTGAAGAAGGAACAACAAGAAAGAGCATAGTAGAATGCTTCAAAACAATGGTTGATGGCATTTGGTCTATTCCAATAAATCTCCCTTTCACAAGGTATAACCACAGCCTGAAAGCAAGTGCAAAAGCTCAACAGATACTGAAGCAACTTTTGAAAGACAAAGCAACAGTAATGGAAGAAGAAAAAGAAAGTGAAGTAGAAGAAAATGATAAAGACCTGATTAGTTATTTGCTTAGAATAAAAAACAAAGACAAGGAACAAGCTTTGAGTGAGGAGGAAATTGTGCATAATATCATCCTTCTAATGATTGCTGGACATGACACAACTTCTATTTTGCTTACTCTAATGCTTAGGGTTTTGGCTACAAACCCAACTGTTTATGCAGCTGTTCTTCAAGAGCATGAAGAAATAGGTAGAAGCAAAGAAAGAGGGGAGGCACTGACTTGGGAAGATATTTCAAAGATGAAGTACACATGGAGAGTAGCAATGGAAACCTTGAGACTTTATCCTCCAGTTTTTGGAGGCTTTAGAGTGGCCCTTAAAGACATTCAATTGGGTGCCTACACAATTCCAAAAGGATGGCAAATCTTTTGGGCAGCACCAATGACACACTTGGATGAGACAATATTTGGAGACCCACAAAAGTTTGAACCAAGTAGATTTGATCAAAACCAAACACCAATTCCACCATTTTCTTTCATTGCTTTTGGTGGAGGACCTAGAATCTGCCCTGGCTATGAATTTGCAAAGCTTGAAACACTTGTTACTATCCATTACCTAATCACTCAATTCACTTGGAACCTCTCCTGTTCACAAGATTTCTTGACTAGAGATCCAACTTTGATGCCTAACAAAGGCCTCCCCATCCAAATTTTTCCCAAATCATAA

Protein sequence

MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFIAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPKS*
Homology
[Close]
BLAST of CSPI03G23250 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 349.7 bits (896), Expect = 5.1e-95
Identity = 186/477 (38.99%), Postives = 291/477 (61.01%), Query Frame = 0 

Query: 6   ILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVDK 65
           ILL+L +F       R    ++  LPPG LG+PFIG+S   L A+R+N+ E++  +RV K
Sbjct: 35  ILLLLLLF-------RSKRHSSLKLPPGKLGIPFIGESFIFLRALRSNSLEQFFDERVKK 94

Query: 66  YGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDH 125
           +G V K +L G PTV + G A N+ ++ S EE  V          ++G+ ++    GEDH
Sbjct: 95  FGLVFKTSLIGHPTVVLCGPAGNR-LILSNEEKLVQMSWPAQFMKLMGENSVATRRGEDH 154

Query: 126 KRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLF 185
             +R AL  F  P  L+SY+GKM+ E++ H+N  W G +EV V PL++ L F+I   L F
Sbjct: 155 IVMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILFF 214

Query: 186 GIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKATVME 245
            I +   +  + +  +T++ G +++PI+LP   ++ +L+  AK  +I+  L+K +    +
Sbjct: 215 NIYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKR----K 274

Query: 246 EEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRV 305
           E+ +S      +DL+S LL  ++ DK   L+ +EI+ N   L+ A +DTT+  + L+ ++
Sbjct: 275 EDLQSGSATATQDLLSVLLTFRD-DKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKL 334

Query: 306 LATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGGFRVA 365
           L++NP  Y  V+QE  EI  +KE GE +TW+D+  MKYTW+VA ETLR++PPVFG FR A
Sbjct: 335 LSSNPECYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKA 394

Query: 366 LKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFIAFGGG 425
           + DIQ   YTIPKGW++ W    TH  +  F +P+KF PSRFDQ    + P++F+ FGGG
Sbjct: 395 ITDIQYDGYTIPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGG 454

Query: 426 PRICPGYEFAKLETLVTIHYLITQFTWNLSCSQD-FLTRDP-TLMPNKGLPIQIFPK 481
            R C G+EF+K+E L+ +H+ +  F+       D  ++ DP   +P+KG  I++FP+
Sbjct: 455 QRSCVGWEFSKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498

[Close]
BLAST of CSPI03G23250 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.1e-92
Identity = 187/480 (38.96%), Postives = 273/480 (56.88%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
           M TV +   + +  F    +   + A + +PPG  G P IG++L  LG  R      +  
Sbjct: 22  MFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFD 81

Query: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
            R  KYG V   +L G PTV       N+  +FS E   V N    S+  +  + +L   
Sbjct: 82  SRTQKYGNVFTTSLVGHPTVVFCSPEGNR-FLFSNENKLVVNSWPSSVGNLF-RSSLITT 141

Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
            G+D KR+R  L+ FLRP+ LR +VG++D   +RHL  +W G +EV   PL+K  TF + 
Sbjct: 142 VGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLA 201

Query: 181 CSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
           C L   I        +   F   V G+  IPI+LP TRYN +  A+   +Q L  ++ ++
Sbjct: 202 CDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINER 261

Query: 241 ATVMEEEKESEVEENDKDLISYLLRIKNKDKE-QALSEEEIVHNIILLMIAGHDTTSILL 300
              +E    S     ++DL+S+LL   N D++ ++L++ EI  NI+LL+ AGHDT+S  L
Sbjct: 262 KIALEAGNASP----EQDLLSFLL--SNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTL 321

Query: 301 TLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVF 360
           T++L+ LA NP  Y  VL+E   I  SKE G+ L WED+ +MKY+WRVA E LRL+P V 
Sbjct: 322 TVLLKFLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQ 381

Query: 361 GGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSF 420
           G FR A+K+     +TIPKGW++ W    TH     F +P+KF+PSRF + + P PP++F
Sbjct: 382 GSFRKAIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRF-EGEGP-PPYTF 441

Query: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFP 480
           + FGGGPR+CPG EFA++E L+ +H ++  F WNL    + +  DP   P  GLPI++ P
Sbjct: 442 VPFGGGPRMCPGNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

[Close]
BLAST of CSPI03G23250 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.8e-92
Identity = 179/483 (37.06%), Postives = 287/483 (59.42%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
           +++ +  +VL + LF R + R    ++  LPPG LG PFIG+SL  L A+R+NT E++L 
Sbjct: 25  VLSAVAGIVLPLLLFLRSKRR----SSVGLPPGKLGYPFIGESLLFLKALRSNTVEQFLD 84

Query: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
           +RV  +G V K +L G PTV + G A N+ ++ + EE  V     +S   ++G++++T  
Sbjct: 85  ERVKNFGNVFKTSLIGHPTVVLCGPAGNR-LILANEEKLVQMSWPKSSMKLMGEKSITAK 144

Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
            GE H  +R AL  F  P  L+ Y+G+M   +  H+N  W GN++V+V  L+  L FDI 
Sbjct: 145 RGEGHMIIRSALQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLVFDIS 204

Query: 181 CSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
             L F I E   R+ + E  + +  G+ ++P++LP   Y+ +L+A +K   IL  L++ +
Sbjct: 205 ACLFFNINEKHERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGLIEKR 264

Query: 241 ATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
               + +  S +  +++DL+S  L  K+ D+    S+EEI+ N   L+   +DTT   + 
Sbjct: 265 ----KMDLSSGLATSNQDLLSVFLTFKD-DRGNPCSDEEILDNFSGLLHGSYDTTVSAMA 324

Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFG 360
            + ++L++NP  Y  V+QE   I  +K  G+ +TW+D+  MKYTW+V  ETLRLYP +FG
Sbjct: 325 CVFKLLSSNPECYEKVVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLYPSIFG 384

Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
            FR A+ DI    Y IPKGW++ W    TH  E  F +P+KF PSRFDQ    + P++F+
Sbjct: 385 SFRQAITDIHYNGYIIPKGWKLLWTPYTTHPKEMYFSEPEKFLPSRFDQEGKLVAPYTFL 444

Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQF-TWNLSCSQDFLTRDPTL-MPNKGLPIQIF 480
            FGGG R CPG+EF+K+E L+++H+ +  F T+      + + RD    +P+ G  +++F
Sbjct: 445 PFGGGQRSCPGWEFSKMEILLSVHHFVKTFSTFTPVDPAEIIARDSLCPLPSNGFSVKLF 497

Query: 481 PKS 482
           P+S
Sbjct: 505 PRS 497

[Close]
BLAST of CSPI03G23250 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.2e-91
Identity = 187/480 (38.96%), Postives = 273/480 (56.88%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
           M TV +   + +  F    +   + A + +PPG  G P IG++L  LG  R      +  
Sbjct: 22  MFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFD 81

Query: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
            R  KYG V   +L G PTV       N+  +FS E   V N    S+  +  + +L   
Sbjct: 82  SRTQKYGNVFTTSLVGHPTVVFCSPEGNR-FLFSNENKLVVNSWPSSVGNLF-RSSLITT 141

Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
            G+D KR+R  L+ FLRP+ LR +VG++D   +RHL  +W G +EVT  PL+K  TF + 
Sbjct: 142 VGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLA 201

Query: 181 CSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
           C L   I        +   F   V G+  IPI+LP TRYN +  A+   +Q L  ++ ++
Sbjct: 202 CDLFASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINER 261

Query: 241 ATVMEEEKESEVEENDKDLISYLLRIKNKDKE-QALSEEEIVHNIILLMIAGHDTTSILL 300
              +E    S     ++DL+S+LL   N D++ ++L++ EI  NI+LL+ AGHDT+S  L
Sbjct: 262 KIGLEAGNASP----EQDLLSFLL--SNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTL 321

Query: 301 TLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVF 360
           T++L+ LA NP  Y  VL+E  +I  SKE G+ L WED+ +MKY+WRVA E LRL+P   
Sbjct: 322 TVLLKFLAENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQ 381

Query: 361 GGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSF 420
           G FR A+K+     +TIPKGW+++W    TH     F +P+ F+PSRF + + P PP++F
Sbjct: 382 GSFRKAIKEFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRF-EGEGP-PPYTF 441

Query: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFP 480
           + FGGGPR+CPG EFA+LE LV +H ++    WNL    + +  DP   P  GLPI++ P
Sbjct: 442 VPFGGGPRMCPGNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVP 491

[Close]
BLAST of CSPI03G23250 vs. ExPASy Swiss-Prot
Match: Q9AXM6 (Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 4.5e-91
Identity = 174/482 (36.10%), Postives = 285/482 (59.13%), Query Frame = 0 

Query: 2   ITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQK 61
           +T+  +L + + L  R+  R    ++  LPPG LG P IG+++ LL  +R+ T +K+   
Sbjct: 26  LTLAPILAIILLLLFRYNHR----SSVKLPPGKLGFPLIGETIQLLRTLRSETPQKFFDD 85

Query: 62  RVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELS 121
           R+ K+GPV   +L G PTV + G A NK +V S E+  V     +S   ++G+ ++    
Sbjct: 86  RLKKFGPVYMTSLIGHPTVVLCGPAGNK-LVLSNEDKLVEMEGPKSFMKLIGEDSIVAKR 145

Query: 122 GEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIIC 181
           GEDH+ +R AL  FL  Q L++Y+G+M  E+  H N  W G +EV V PL++ L F I  
Sbjct: 146 GEDHRILRTALARFLGAQALQNYLGRMSSEIGHHFNEKWKGKDEVKVLPLVRGLIFSIAS 205

Query: 182 SLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKA 241
           +L F + +G  +K +    +T++ G  S+P++ P TRY   L+A  K  +IL  L+K + 
Sbjct: 206 TLFFDVNDGHQQKQLHHLLETILVGSLSVPLDFPGTRYRKGLQARLKLDEILSSLIKRR- 265

Query: 242 TVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTL 301
                +  S +  +D+DL+S LL  ++ +K  +L+++ I+ N   +  A +DTT   + L
Sbjct: 266 ---RRDLRSGIASDDQDLLSVLLTFRD-EKGNSLTDQGILDNFSAMFHASYDTTVAPMAL 325

Query: 302 MLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGG 361
           + ++L +NP  +  V QE  EI  +K+ GE ++W+D+  MKYTW+   E+LR+YPPVFG 
Sbjct: 326 IFKLLYSNPEYHEKVFQEQLEIIGNKKEGEEISWKDLKSMKYTWQAVQESLRMYPPVFGI 385

Query: 362 FRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFIA 421
           FR A+ DI    YTIPKGW++  +   THL E  F +P++F PSRF+     + P++++ 
Sbjct: 386 FRKAITDIHYDGYTIPKGWRVLCSPYTTHLREEYFPEPEEFRPSRFEDEGRHVTPYTYVP 445

Query: 422 FGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSC--SQDFLTRDPTLMPNKGLPIQIFP 481
           FGGG R CPG+EF+K+E L+ +H+ +  F+  +    ++  L+     +P  G  I++FP
Sbjct: 446 FGGGLRTCPGWEFSKIEILLFVHHFVKNFSSYIPVDPNEKVLSDPLPPLPANGFSIKLFP 497

[Close]
BLAST of CSPI03G23250 vs. ExPASy TrEMBL
Match: A0A0A0L864 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G416660 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.0e-276
Identity = 478/481 (99.38%), Postives = 480/481 (99.79%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
           MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ
Sbjct: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60

Query: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
           KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL
Sbjct: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120

Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
           SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180

Query: 181 CSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
           CSLLFGIEEGTTRKSI+ECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK
Sbjct: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240

Query: 241 ATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
           A VMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT
Sbjct: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300

Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFG 360
           LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWED+SKMKYTWRVAMETLRLYPPVFG
Sbjct: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360

Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
           GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI
Sbjct: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420

Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
           AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480

Query: 481 S 482
           S
Sbjct: 481 S 481

[Close]
BLAST of CSPI03G23250 vs. ExPASy TrEMBL
Match: A0A1S3C037 (LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103494945 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 4.9e-258
Identity = 446/481 (92.72%), Postives = 465/481 (96.67%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
           MIT IILLV+TIFLFHRFRTR SSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ
Sbjct: 1   MITPIILLVITIFLFHRFRTRFSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60

Query: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
           +RVDKYGPVSKMTLFGKPTVF+HGAAANKAVVFSGEEGTVSNRQ+ESLKMILGKRNLTEL
Sbjct: 61  QRVDKYGPVSKMTLFGKPTVFVHGAAANKAVVFSGEEGTVSNRQIESLKMILGKRNLTEL 120

Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
           SGEDHKRVRGALVWFLRPQTLRSYV KMDGEVRRHLNMYWHGNN+VTVAPLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQTLRSYVEKMDGEVRRHLNMYWHGNNKVTVAPLMKTLTFDII 180

Query: 181 CSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
           CSLLFGIEEGTTRKSI++CFKTMV+GIWS+PINLPFTRYNHSLKASAK QQILKQLLKDK
Sbjct: 181 CSLLFGIEEGTTRKSIIQCFKTMVEGIWSVPINLPFTRYNHSLKASAKVQQILKQLLKDK 240

Query: 241 ATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
           A  ME++K  ++++  KDLI+YLLRIKNKDKEQAL+EEEI+HNIILLMIAGHDTTSILLT
Sbjct: 241 AKRMEQKK--KMKKKMKDLITYLLRIKNKDKEQALTEEEILHNIILLMIAGHDTTSILLT 300

Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFG 360
           LMLRVLATNPTVYA VLQEHEEI RSKERGEALTWED+SKMKYTWRVAMETLRLYPPVFG
Sbjct: 301 LMLRVLATNPTVYADVLQEHEEIARSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360

Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
           GFRVALKDIQLGAYTIPKGWQIFWAA  THLDETIFGDPQKFEPSRFDQ QTPIPPFSFI
Sbjct: 361 GFRVALKDIQLGAYTIPKGWQIFWAASTTHLDETIFGDPQKFEPSRFDQTQTPIPPFSFI 420

Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
           AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQD LTRDPTLMPNKGLPIQIFPK
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDSLTRDPTLMPNKGLPIQIFPK 479

Query: 481 S 482
           S
Sbjct: 481 S 479

[Close]
BLAST of CSPI03G23250 vs. ExPASy TrEMBL
Match: A0A6J1INM7 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111476875 PE=3 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.8e-229
Identity = 401/477 (84.07%), Postives = 437/477 (91.61%), Query Frame = 0 

Query: 6   ILLVLTIFLFHRFR-TRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVD 65
           ILL++TIFL+ R R T  SS+ AKNLPPG LGVPFIGQSLSLL AMR N+AE+WLQ+RV 
Sbjct: 5   ILLLITIFLYLRLRATFFSSAEAKNLPPGPLGVPFIGQSLSLLRAMRANSAEQWLQQRVA 64

Query: 66  KYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGED 125
           KYGP+SKMTLFGKPTVF+HG AANKAVVFSGEE  VSNRQ ESL+ ILG+RNLTELSGED
Sbjct: 65  KYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTELSGED 124

Query: 126 HKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLL 185
           HKRVRGALVWFLRPQ+L+ +VGKMDGEVR+HLN++WHGN EV VAPLMKTLTFDIICSLL
Sbjct: 125 HKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLL 184

Query: 186 FGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKATVM 245
           FGIEEG TRKSI+ECFKTMVDGIWS+PINLPFTRY+HSL ASAK QQILKQLLKDKA  M
Sbjct: 185 FGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLNASAKVQQILKQLLKDKAQKM 244

Query: 246 EEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLR 305
           EEE+E   +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLMLR
Sbjct: 245 EEEEEE--KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLR 304

Query: 306 VLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGGFRV 365
           V+ATNPTVYAAVLQEHE+I RSKE GEALTWED+SKMKYTWRVA+ETLRL+PPVFGGFRV
Sbjct: 305 VMATNPTVYAAVLQEHEDIHRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRV 364

Query: 366 ALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIAFG 425
           ALKDIQLG YTIPKGWQIFWAA MTHLDE IF DPQKF+PSRFDQNQT I PP SFIAFG
Sbjct: 365 ALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFTDPQKFKPSRFDQNQTSIPPPLSFIAFG 424

Query: 426 GGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
           GGPRICPGYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 425 GGPRICPGYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 478

[Close]
BLAST of CSPI03G23250 vs. ExPASy TrEMBL
Match: A0A6J1F7G6 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443055 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 5.3e-228
Identity = 400/479 (83.51%), Postives = 437/479 (91.23%), Query Frame = 0 

Query: 5   IILLVLTIFLFHRFRTRLSSS--AAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKR 64
           IILL++TIF++ R RT   S+  AAKNLPPG LGVP+IGQSLSLL AMR N+AE+WLQ+R
Sbjct: 4   IILLLITIFVYLRLRTTFFSTAVAAKNLPPGRLGVPYIGQSLSLLRAMRANSAEQWLQQR 63

Query: 65  VDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSG 124
           V KYGP+SKMTLFGKPTVF+HG AANKAVVFSGEE  VSNRQ ESL+ ILG+RNLTELSG
Sbjct: 64  VAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTELSG 123

Query: 125 EDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICS 184
           EDHKRVRGALVWFLRPQ+L+ +VGKMDGEVR+HL ++WHGN EV VAPLMKTLTFDIICS
Sbjct: 124 EDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLKLHWHGNKEVIVAPLMKTLTFDIICS 183

Query: 185 LLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKAT 244
           LLFGIEEG TRKSI+ECFKTMVDGIWS+PINLPFTRY+HSLKASAK QQILKQLLKDKA 
Sbjct: 184 LLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQ 243

Query: 245 VMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLM 304
            ME E+E   +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLM
Sbjct: 244 KMEGEEEE--KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLM 303

Query: 305 LRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGGF 364
           LRV+ATNPTVYAAVLQEHE+I RSKE GEALTWED+SKMKYTWRVA+ETLRL+PPVFGGF
Sbjct: 304 LRVMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGF 363

Query: 365 RVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIA 424
           RVALKDIQLG YTIPKGWQIFWAA MTHLDE IF DPQKFEPSRFDQNQT I PP SFIA
Sbjct: 364 RVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIA 423

Query: 425 FGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
           FGGGPRICPGYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 424 FGGGPRICPGYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 479

[Close]
BLAST of CSPI03G23250 vs. ExPASy TrEMBL
Match: A0A6J1CCZ3 (taxadiene 5-alpha hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111010356 PE=3 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 1.0e-207
Identity = 363/480 (75.62%), Postives = 413/480 (86.04%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
           +I +  L +LTIF       R  SS    LPPG LG+P+IGQSL LL AMR+N+AE+WL 
Sbjct: 2   IIPMFFLFLLTIFFLFLTTRRTRSS---RLPPGPLGLPYIGQSLGLLRAMRSNSAEQWLL 61

Query: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
           +RV KYGPVSKMTLFGKPTVFIHG  ANKA VF+GEEG +SN+Q ESLK ILG+RNLTEL
Sbjct: 62  RRVAKYGPVSKMTLFGKPTVFIHGTVANKA-VFTGEEGAMSNQQTESLKRILGERNLTEL 121

Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
           SGEDHKRVRGALV FL+P +L+ YVGKMDGE+R+HL MYWH N EVTV PLMK LTFDII
Sbjct: 122 SGEDHKRVRGALVSFLKPDSLKKYVGKMDGEIRKHLQMYWHDNKEVTVLPLMKILTFDII 181

Query: 181 CSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
           CSLLFGIEEG  RKS+V CFKTMVDGIWS+PINLPFTRY+HSLKASAK QQ+LKQLLK+K
Sbjct: 182 CSLLFGIEEGPRRKSMVGCFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQMLKQLLKEK 241

Query: 241 ATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
              ME+E +   EE  +DL++ LL +KNKDKEQALSE+EIVHNII+LM+AGHDTT+IL+T
Sbjct: 242 REKMEKEDQ---EEEQQDLVTCLLSMKNKDKEQALSEDEIVHNIIILMVAGHDTTTILIT 301

Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFG 360
           LM+RVL +NP VYAAVLQEHEEI RSKERGE L+WED+ KMKYTWRVA+ETLRL+PPVFG
Sbjct: 302 LMIRVLGSNPAVYAAVLQEHEEIARSKERGEPLSWEDVGKMKYTWRVALETLRLFPPVFG 361

Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
           GFRVAL+DI+ G YTIPKGWQ+FWAAPMTHLD+TIF DP KFEP+RFD+ QT IPP  FI
Sbjct: 362 GFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDDTIFADPHKFEPNRFDK-QTLIPPLCFI 421

Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
           AFGGGPRICPG EFAKLETL+TIHYLITQFTW L CS++FLTRDPTLMPNKGLPIQI+PK
Sbjct: 422 AFGGGPRICPGSEFAKLETLITIHYLITQFTWKL-CSEEFLTRDPTLMPNKGLPIQIYPK 472

[Close]
BLAST of CSPI03G23250 vs. NCBI nr
Match: XP_004152028.1 (taxadiene 5-alpha hydroxylase [Cucumis sativus] >KGN57973.1 hypothetical protein Csa_010468 [Cucumis sativus])

HSP 1 Score: 960.3 bits (2481), Expect = 6.3e-276
Identity = 478/481 (99.38%), Postives = 480/481 (99.79%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
           MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ
Sbjct: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60

Query: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
           KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL
Sbjct: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120

Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
           SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180

Query: 181 CSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
           CSLLFGIEEGTTRKSI+ECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK
Sbjct: 181 CSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240

Query: 241 ATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
           A VMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT
Sbjct: 241 AKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300

Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFG 360
           LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWED+SKMKYTWRVAMETLRLYPPVFG
Sbjct: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360

Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
           GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI
Sbjct: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420

Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
           AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480

Query: 481 S 482
           S
Sbjct: 481 S 481

[Close]
BLAST of CSPI03G23250 vs. NCBI nr
Match: XP_008454553.1 (PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like [Cucumis melo])

HSP 1 Score: 899.8 bits (2324), Expect = 1.0e-257
Identity = 446/481 (92.72%), Postives = 465/481 (96.67%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
           MIT IILLV+TIFLFHRFRTR SSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ
Sbjct: 1   MITPIILLVITIFLFHRFRTRFSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60

Query: 61  KRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTEL 120
           +RVDKYGPVSKMTLFGKPTVF+HGAAANKAVVFSGEEGTVSNRQ+ESLKMILGKRNLTEL
Sbjct: 61  QRVDKYGPVSKMTLFGKPTVFVHGAAANKAVVFSGEEGTVSNRQIESLKMILGKRNLTEL 120

Query: 121 SGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDII 180
           SGEDHKRVRGALVWFLRPQTLRSYV KMDGEVRRHLNMYWHGNN+VTVAPLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQTLRSYVEKMDGEVRRHLNMYWHGNNKVTVAPLMKTLTFDII 180

Query: 181 CSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDK 240
           CSLLFGIEEGTTRKSI++CFKTMV+GIWS+PINLPFTRYNHSLKASAK QQILKQLLKDK
Sbjct: 181 CSLLFGIEEGTTRKSIIQCFKTMVEGIWSVPINLPFTRYNHSLKASAKVQQILKQLLKDK 240

Query: 241 ATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLT 300
           A  ME++K  ++++  KDLI+YLLRIKNKDKEQAL+EEEI+HNIILLMIAGHDTTSILLT
Sbjct: 241 AKRMEQKK--KMKKKMKDLITYLLRIKNKDKEQALTEEEILHNIILLMIAGHDTTSILLT 300

Query: 301 LMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFG 360
           LMLRVLATNPTVYA VLQEHEEI RSKERGEALTWED+SKMKYTWRVAMETLRLYPPVFG
Sbjct: 301 LMLRVLATNPTVYADVLQEHEEIARSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFG 360

Query: 361 GFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFI 420
           GFRVALKDIQLGAYTIPKGWQIFWAA  THLDETIFGDPQKFEPSRFDQ QTPIPPFSFI
Sbjct: 361 GFRVALKDIQLGAYTIPKGWQIFWAASTTHLDETIFGDPQKFEPSRFDQTQTPIPPFSFI 420

Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 480
           AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQD LTRDPTLMPNKGLPIQIFPK
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDSLTRDPTLMPNKGLPIQIFPK 479

Query: 481 S 482
           S
Sbjct: 481 S 479

[Close]
BLAST of CSPI03G23250 vs. NCBI nr
Match: XP_038899676.1 (taxadiene 5-alpha hydroxylase-like [Benincasa hispida])

HSP 1 Score: 866.3 bits (2237), Expect = 1.2e-247
Identity = 433/481 (90.02%), Postives = 454/481 (94.39%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSA-AKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWL 60
           MIT+ +LL+ TIFLF RFRT  SS+A AKNLPPG LGVPFIGQSLSLL AMRTN+AE+WL
Sbjct: 1   MITIFLLLI-TIFLFLRFRTTSSSAATAKNLPPGPLGVPFIGQSLSLLRAMRTNSAEQWL 60

Query: 61  QKRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTE 120
           QKRV KYGPVSKMTLFGKPTVF+HGAAANKAVVF GEEGTVSNRQ+ESLK ILG+RNLTE
Sbjct: 61  QKRVAKYGPVSKMTLFGKPTVFVHGAAANKAVVFLGEEGTVSNRQIESLKWILGERNLTE 120

Query: 121 LSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDI 180
           LSGEDHKRVRGALVWFLRPQTL+SYVGKMDGEVR+HLNMYWHG  EVTVAPLMKTLTFDI
Sbjct: 121 LSGEDHKRVRGALVWFLRPQTLKSYVGKMDGEVRKHLNMYWHGKKEVTVAPLMKTLTFDI 180

Query: 181 ICSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKD 240
           ICSLLFGIEEG TRKSI++CFKTMVDGIWS+PINLPFTRYNHSLKASAKAQQILKQLLKD
Sbjct: 181 ICSLLFGIEEGPTRKSIIQCFKTMVDGIWSVPINLPFTRYNHSLKASAKAQQILKQLLKD 240

Query: 241 KATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILL 300
           KA  MEEE+E E +E+DKDLI+YLL IKNKDKEQALSEEEIVHN ILLMIAGHDTTSILL
Sbjct: 241 KAQKMEEEEEEE-KEDDKDLITYLLSIKNKDKEQALSEEEIVHNTILLMIAGHDTTSILL 300

Query: 301 TLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVF 360
           TLMLRVLATNPTVYAAVLQEHEEI RSKE GEALTWED+SKMKYTWRVAMETLR+YPPVF
Sbjct: 301 TLMLRVLATNPTVYAAVLQEHEEIARSKESGEALTWEDVSKMKYTWRVAMETLRIYPPVF 360

Query: 361 GGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSF 420
           GGFRVALKDIQLG YTIPKGWQIFWAAPMTHLDETIFG+PQKFEPSRFDQNQTPIPPF F
Sbjct: 361 GGFRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGEPQKFEPSRFDQNQTPIPPFCF 420

Query: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFP 480
           IAFGGGPRICPG EFAKLETLVTIHYLITQFTW LSCSQDFLTRDPTLMPNKGLPIQI P
Sbjct: 421 IAFGGGPRICPGSEFAKLETLVTIHYLITQFTWKLSCSQDFLTRDPTLMPNKGLPIQISP 479

[Close]
BLAST of CSPI03G23250 vs. NCBI nr
Match: XP_023535435.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 807.0 bits (2083), Expect = 8.9e-230
Identity = 401/477 (84.07%), Postives = 438/477 (91.82%), Query Frame = 0 

Query: 5   IILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVD 64
           IILL++TIFL+ R RT   S+AAKNLPPG LGVP+IGQSLSLL AMR N+AE+WLQ+RV 
Sbjct: 4   IILLLITIFLYLRLRTTFFSAAAKNLPPGRLGVPYIGQSLSLLRAMRANSAEQWLQQRVA 63

Query: 65  KYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGED 124
           KYGP+SKMTLFGKPTVF+HG AANKAVVF GEE  VSNRQ ESL+ ILG+RNLTELSGED
Sbjct: 64  KYGPISKMTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTELSGED 123

Query: 125 HKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLL 184
           HKRVRGALVWFLRPQ+L+ +VGKMDGEVR+HLN++WHGN EV VAPLMKTLTFDIICSLL
Sbjct: 124 HKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLL 183

Query: 185 FGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKATVM 244
           FGIEEG TRKSI+ECFKTMVDGIWS+PINLPFTRY+HSLKASAK QQILKQLLKDKA  M
Sbjct: 184 FGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKM 243

Query: 245 EEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLR 304
           EEE+E   +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLMLR
Sbjct: 244 EEEEE---KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLR 303

Query: 305 VLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGGFRV 364
           V+ATNPTVYAAVLQEHE+I RSKE GEALTWED+SKMKYTWRVA+ETLRL+PPVFGGFR+
Sbjct: 304 VMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRM 363

Query: 365 ALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIAFG 424
           ALKDIQLG YTIPKGWQIFWAA MTHLDE IF DPQKFEPSRFDQNQT I PP SFIAFG
Sbjct: 364 ALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFG 423

Query: 425 GGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
           GGPRICPGYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 424 GGPRICPGYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 476

[Close]
BLAST of CSPI03G23250 vs. NCBI nr
Match: XP_022976494.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 803.5 bits (2074), Expect = 9.8e-229
Identity = 401/477 (84.07%), Postives = 437/477 (91.61%), Query Frame = 0 

Query: 6   ILLVLTIFLFHRFR-TRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVD 65
           ILL++TIFL+ R R T  SS+ AKNLPPG LGVPFIGQSLSLL AMR N+AE+WLQ+RV 
Sbjct: 5   ILLLITIFLYLRLRATFFSSAEAKNLPPGPLGVPFIGQSLSLLRAMRANSAEQWLQQRVA 64

Query: 66  KYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGED 125
           KYGP+SKMTLFGKPTVF+HG AANKAVVFSGEE  VSNRQ ESL+ ILG+RNLTELSGED
Sbjct: 65  KYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTELSGED 124

Query: 126 HKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLL 185
           HKRVRGALVWFLRPQ+L+ +VGKMDGEVR+HLN++WHGN EV VAPLMKTLTFDIICSLL
Sbjct: 125 HKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLL 184

Query: 186 FGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKATVM 245
           FGIEEG TRKSI+ECFKTMVDGIWS+PINLPFTRY+HSL ASAK QQILKQLLKDKA  M
Sbjct: 185 FGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLNASAKVQQILKQLLKDKAQKM 244

Query: 246 EEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLR 305
           EEE+E   +E DK+LI+ LL IKNKDKEQALSEEEIVHNIILL+IAGHDTTSIL+TLMLR
Sbjct: 245 EEEEEE--KEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLMLR 304

Query: 306 VLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGGFRV 365
           V+ATNPTVYAAVLQEHE+I RSKE GEALTWED+SKMKYTWRVA+ETLRL+PPVFGGFRV
Sbjct: 305 VMATNPTVYAAVLQEHEDIHRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFRV 364

Query: 366 ALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPI-PPFSFIAFG 425
           ALKDIQLG YTIPKGWQIFWAA MTHLDE IF DPQKF+PSRFDQNQT I PP SFIAFG
Sbjct: 365 ALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFTDPQKFKPSRFDQNQTSIPPPLSFIAFG 424

Query: 426 GGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
           GGPRICPGYEFAKLETLVTIHY+ITQFTW LS SQDFLTRDP+LMPNKGLPIQ++PK
Sbjct: 425 GGPRICPGYEFAKLETLVTIHYIITQFTWKLS-SQDFLTRDPSLMPNKGLPIQLYPK 478

[Close]
BLAST of CSPI03G23250 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 325.1 bits (832), Expect = 9.6e-89
Identity = 180/486 (37.04%), Postives = 285/486 (58.64%), Query Frame = 0 

Query: 1   MITVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQ 60
           ++ ++ L  + + L    R  LS  +  NLPPG  G+P IG+S S L A R    EK++ 
Sbjct: 5   IMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKFIT 64

Query: 61  KRVDKYGP----VSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRN 120
            RV ++      V K  LFG PT  + GA+ NK  +F+ E   V +   +S+  I     
Sbjct: 65  DRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNK-FLFTNENKLVVSWWPDSVNKIFPSSM 124

Query: 121 LTELSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLT 180
            T  S E+ +++R  L  F++P+ LR YVG MD   +RH    W   ++V V PL K  T
Sbjct: 125 QTS-SKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFT 184

Query: 181 FDIICSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQL 240
           F I C     +E+    + + E F T+  GI+SIPI+LP TR+N ++KAS   ++ +  +
Sbjct: 185 FSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAI 244

Query: 241 LKDKATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTS 300
           ++ +    +EE ++     + D++S++L    + K     +E++   II L+I GHDT S
Sbjct: 245 VRQR----KEELKAGKALEEHDILSHMLMNIGETK-----DEDLADKIIGLLIGGHDTAS 304

Query: 301 ILLTLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYP 360
           I+ T ++  LA  P VY  VLQE +EI + K+  E L WEDI KM+Y+W VA E +R+ P
Sbjct: 305 IVCTFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVP 364

Query: 361 PVFGGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPP 420
           P+ G FR A+       + IPKGW+++W+A  TH++   F +P++FEP+RF+ +     P
Sbjct: 365 PLSGTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGP--KP 424

Query: 421 FSFIAFGGGPRICPGYEFAKLETLVTIHYLITQFTW-NLSCSQDFLTRDPTLMPNKGLPI 480
           ++++ FGGGPR+CPG E+A+LE L+ +H L+ +F W  +  +++ +  DP  +P+KGLPI
Sbjct: 425 YTYVPFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPI 477

Query: 481 QIFPKS 482
           +IFP+S
Sbjct: 485 RIFPQS 477

[Close]
BLAST of CSPI03G23250 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-86
Identity = 167/475 (35.16%), Postives = 275/475 (57.89%), Query Frame = 0 

Query: 11  TIFLF----HRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTA-EKWLQKRVDK 70
           T+F F    HRF T+      K L PG +G+P+IG+++    A ++N   E ++  R+ K
Sbjct: 23  TLFFFRKKHHRFITK-KIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIK 82

Query: 71  YGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDH 130
           +G + K  + G PT+ ++GA AN+ ++ S E   V +    S   ++G   +    GE H
Sbjct: 83  HGNIFKTRIMGSPTIVVNGAEANR-LILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKH 142

Query: 131 KRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLF 190
           + +RG +   L    L S + K+   V+ H    W G  E+++    K LTF ++   L+
Sbjct: 143 RVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLY 202

Query: 191 GIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKATVME 250
           GI+       ++E F+ +++G++++P+  P +++  + KA  + +  L         V E
Sbjct: 203 GIK---VEIGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFL------VGKVRE 262

Query: 251 EEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRV 310
           + +E E E  +K   +   R+  +  +  ++EEE+V N++LL+ A HDTTS  +++  ++
Sbjct: 263 KRREMEKEGAEKPNTTLFSRLVEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKM 322

Query: 311 LATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGGFRVA 370
           LA +PT    +LQEH +I  +K  GE LT ED+ KMKY+W+V  ET+RL PP+FG FR A
Sbjct: 323 LAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKA 382

Query: 371 LKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFIAFGGG 430
           + DI  G YTIPKGW+I W    TH +  IF DP  F+P+RFD+   PI  ++++ FGGG
Sbjct: 383 VADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDK---PIQAYTYLPFGGG 442

Query: 431 PRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFPK 481
           PR+C G++ AK+  LV +H+++T F W+L    + ++ DP   P+ G+PI+I PK
Sbjct: 443 PRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPK 483

[Close]
BLAST of CSPI03G23250 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 2.1e-64
Identity = 151/475 (31.79%), Postives = 246/475 (51.79%), Query Frame = 0 

Query: 3   TVIILLVLTIFLFHRFRTRLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKR 62
           T  +LL+ +I        R +      LPPG+LG+P IG++  L+GA +T   E ++ +R
Sbjct: 4   TAFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDER 63

Query: 63  VDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSG 122
           V +YG V    LFG+PT+F      N+  V   E          S+  +LGK +L  + G
Sbjct: 64  VARYGSVFMTHLFGEPTIFSADPETNR-FVLQNEGKLFECSYPASICNLLGKHSLLLMKG 123

Query: 123 EDHKRVRGALVWFLRPQTLRSYVG-KMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIIC 182
             HKR+    + F     ++ ++   +D  VR +L+ +   ++ V +    K +TF++  
Sbjct: 124 SLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSW---SSRVLLMEEAKKITFELTV 183

Query: 183 SLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKA 242
             L   + G   +S+ + +  +++G +S+P+ L  T Y  +++A  K  + L  ++    
Sbjct: 184 KQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVV---- 243

Query: 243 TVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTL 302
            +   E+E E  E  KD+++ LL       +   S+EEIV  ++ L++AG++TTS ++TL
Sbjct: 244 -MKRREEEEEGAERKKDMLAALLA-----ADDGFSDEEIVDFLVALLVAGYETTSTIMTL 303

Query: 303 MLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGG 362
            ++ L   P   A + +EHE+I   K    +L W D   M +T  V  ETLR+   + G 
Sbjct: 304 AVKFLTETPLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGV 363

Query: 363 FRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFS-FI 422
           FR A+ D+++  Y IPKGW++F +    HLD   F D + F P R+  N     P + F 
Sbjct: 364 FRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFT 423

Query: 423 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPI 476
            FGGGPR+CPGYE A++   V +H L+T F+W +   QD L   PT    K  PI
Sbjct: 424 PFGGGPRLCPGYELARVALSVFLHRLVTGFSW-VPAEQDKLVFFPTTRTQKRYPI 463

[Close]
BLAST of CSPI03G23250 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-62
Identity = 150/457 (32.82%), Postives = 234/457 (51.20%), Query Frame = 0 

Query: 21  RLSSSAAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVDKYGPVSKMTLFGKPTV 80
           R SSS    LPPG +G P++G++  L     +     +   +  +YG V K  + G P V
Sbjct: 27  RRSSSTKLPLPPGTMGYPYVGETFQLY----SQDPNVFFAAKQRRYGSVFKTHVLGCPCV 86

Query: 81  FIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDHKRVRGALVWFLRPQT 140
            I    A K V+ + +          S + +LGK+ +    G+ H ++R  ++    P  
Sbjct: 87  MISSPEAAKFVLVT-KSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDA 146

Query: 141 LRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLFGIEEGTTRKSIVECF 200
           +R+ V  ++   +  LN  W G  ++     MKT TF++    + G +E   R+ +  C+
Sbjct: 147 IRNMVPHIESIAQESLNS-WDG-TQLNTYQEMKTYTFNVALISILGKDEVYYREDLKRCY 206

Query: 201 KTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKDKATVMEEEKESEVEENDKDLI 260
             +  G  S+PINLP T ++ ++KA  +  QIL  +L          K  +   +  DL+
Sbjct: 207 YILEKGYNSMPINLPGTLFHKAMKARKELAQILANIL---------SKRRQNPSSHTDLL 266

Query: 261 SYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRVLATNPTVYAAVLQEH 320
              +     + +  L++E+I  NII ++ A  DTT+ +LT +L+ LA NPTV  AV +E 
Sbjct: 267 GSFM-----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQ 326

Query: 321 EEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYPPVFGGFRVALKDIQLGAYTIPKGW 380
             I + K+ GE+LTWED  KM  T+RV  ETLR    +   FR A++D++   Y IPKGW
Sbjct: 327 MAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGW 386

Query: 381 QIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSFIAFGGGPRICPGYEFAKLETL 440
           ++       H +  IF DP KF+PSRF   +    P +F+ FG G   CPG E AKLE  
Sbjct: 387 KVLPLFRNIHHNADIFSDPGKFDPSRF---EVAPKPNTFMPFGSGIHSCPGNELAKLEIS 446

Query: 441 VTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQI 478
           V IH+L T++ W++    D +   P  +P  GLPI +
Sbjct: 447 VLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459

[Close]
BLAST of CSPI03G23250 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 232.3 bits (591), Expect = 8.4e-61
Identity = 159/484 (32.85%), Postives = 249/484 (51.45%), Query Frame = 0 

Query: 5   IILLVLTIF---LFHRFRTRLSS-----SAAKNLPPGALGVPFIGQSLSLLGAMRTNTAE 64
           I  L LT+F   LF  F   L S     S+   LPPG +G P++G++  L     +    
Sbjct: 3   ISALFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY----SQDPN 62

Query: 65  KWLQKRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRN 124
            + Q +  +YG V K  + G P V I    A K V+ + +          S + +LGK+ 
Sbjct: 63  VFFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVT-KSHLFKPTFPASKERMLGKQA 122

Query: 125 LTELSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLT 184
           +    G+ H ++R  ++    P+++R+ V  ++   +  L   W G   +     MKT T
Sbjct: 123 IFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRS-WEG-TMINTYQEMKTYT 182

Query: 185 FDIICSLLFGIEEGTTRKSIVECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQL 244
           F++    +FG +E   R+ +  C+  +  G  S+P+NLP T ++ S+KA  +  QIL   
Sbjct: 183 FNVALLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQIL--- 242

Query: 245 LKDKATVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTS 304
               A ++ E +++    ND      LL     DKE+ L++E+I  NII ++ A  DTT+
Sbjct: 243 ----ARILSERRQNGSSHND------LLGSFMGDKEE-LTDEQIADNIIGVIFAARDTTA 302

Query: 305 ILLTLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDISKMKYTWRVAMETLRLYP 364
            +++ +L+ LA NP V  AV +E   I + KE GE+LTW D  KM  T RV  ETLR+  
Sbjct: 303 SVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVAS 362

Query: 365 PVFGGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPP 424
            +   FR A++D++   Y IPKGW++       H    IF +P KF+PSRF   +    P
Sbjct: 363 ILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRF---EVAPKP 422

Query: 425 FSFIAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQ 481
            +F+ FG G   CPG E AKLE  + IH+L T+++W++  + D +   P  +P  GLPI 
Sbjct: 423 NTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPIV 462

The following BLAST results are available for this feature:
BLAST of CSPI03G23250 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q6WG305.1e-9538.99Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2[more]
Q50EK11.1e-9238.96Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1[more]
Q84KI11.8e-9237.06Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1[more]
Q50EK01.2e-9138.96Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1[more]
Q9AXM64.5e-9136.10Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1[more]
BLAST of CSPI03G23250 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8643.0e-27699.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G416660 PE=3 SV=1[more]
A0A1S3C0374.9e-25892.72LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like OS=Cucumis melo OX=3656 ... [more]
A0A6J1INM74.8e-22984.07taxadiene 5-alpha hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111476875 P... [more]
A0A6J1F7G65.3e-22883.51taxadiene 5-alpha hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443055... [more]
A0A6J1CCZ31.0e-20775.63taxadiene 5-alpha hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC11101035... [more]
BLAST of CSPI03G23250 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004152028.16.3e-27699.38taxadiene 5-alpha hydroxylase [Cucumis sativus] >KGN57973.1 hypothetical protein... [more]
XP_008454553.11.0e-25792.72PREDICTED: LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like [Cucumis melo... [more]
XP_038899676.11.2e-24790.02taxadiene 5-alpha hydroxylase-like [Benincasa hispida][more]
XP_023535435.18.9e-23084.07taxadiene 5-alpha hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_022976494.19.8e-22984.07taxadiene 5-alpha hydroxylase-like [Cucurbita maxima][more]
BLAST of CSPI03G23250 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G36110.19.6e-8937.04cytochrome P450, family 716, subfamily A, polypeptide 1 [more]
AT2G42850.12.0e-8635.16cytochrome P450, family 718 [more]
AT5G05690.12.1e-6431.79Cytochrome P450 superfamily protein [more]
AT5G45340.11.2e-6232.82cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT4G19230.18.4e-6132.85cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 234..254
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 7..453
e-value: 1.4E-9
score: 35.3
NoneNo IPR availablePANTHERPTHR24286:SF237OS07G0520300 PROTEINcoord: 6..478
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 6..478
IPR001128Cytochrome P450PRINTSPR00385P450coord: 420..429
score: 54.2
coord: 290..307
score: 38.46
coord: 346..357
score: 41.42
coord: 429..440
score: 36.51
IPR001128Cytochrome P450PFAMPF00067p450coord: 31..471
e-value: 1.1E-73
score: 248.6
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 63..82
score: 28.72
coord: 299..325
score: 21.56
coord: 429..452
score: 25.19
coord: 385..409
score: 27.93
coord: 419..429
score: 49.4
coord: 175..193
score: 23.1
coord: 279..296
score: 31.59
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 20..478
e-value: 3.3E-123
score: 413.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..479
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 422..431

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G23250.1CSPI03G23250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen