CSPI03G23000 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G23000
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	glycine-rich RNA-binding protein 4, mitochondrial
Location	Chr3: 19762731 .. 19765769 (+)
RNA-Seq Expression	CSPI03G23000
Synteny	CSPI03G23000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGCGTTAAGAAGAAGAGTCCTCCTTCCTCAAAACGAAAAAGAAAAAAAATCTGTTTTTCCTCGCCTCTTCCAGAAAGGGTTTAGTACCTTAAACCCCAATCCTATGGCTCTCAGATCGGCGGCGGCGTCGGCTCCTCGGAGTTTGAGGAAACTCATTACACGTACCTCAACTCGTACTCTACCGATAATCTCCCAGGCTATTCATGATTGGGAGAAACCGGAGCCCAGCACCATTACTTTCCTTTCTGGCCGCCTGTTCTCCAGCGCTTCTACTTCTAGTTTCATTCCTCCGCCGCTCCCTCCCTCGGCTCAGCAGCGTGAGGTGGCTGAGCCCAGTACTAACCTTTTCGTTTCCGGTAAATCGTTCTTTCTTAATCTATCTGCCTTGCGTTTATTTACTTTAACAATCATGTATTTGCGTTATGTATATTGCTCCCTTTCTGTTCTGAAACTGCTAACGTTATTTTGATTTATGGTTTCATTCTTGCCAATTTTTTTTGGAGTCTGGAATTTCATTTTTCCTGTTCTTCTTTTGGTCCTATTTTAGCTGTCTTAGTAATTGAGAAATGCTTAGTTACCTTTTTTTTTTAATAAATATTTTCTAACCTTCGATTGTCAATTTTATGGTATCTTTTTAGTTAGTGTTGAGATATCTTTTTTTATTTTTTATGCCTCTTCTTGTTCTTTGCCTTTAATTTGCTTCACCTCTCGGTTTTGAATTGTTGAGATGCATACGTATATTTCTAGATGGGGAAGTTTAGTAATTGGTGGAATAGTAAGTATTGTGAGTACAATCTATGCTTGACTGGACCTAATGAGGTGGCTATCCATTCTCTTTGGATGTTGGCCTCCTCTACGTTTATCTTTTTCAATGATCGATTCTTGGAAGCTGGAATTGAAATTTGGCTAATGTGTTTGTTGAGCTGTGTTTCTGTTTCTTTTCAAGTAGAGGAATTGTTCTGTCTTTTAATACAGAATTTTCAAGTTTTATTGTGTTTATGAAATTAATGAACTGTTTGTGAAGCACGTAATTGTTGATGTTTATCTTCTTTCAGGCCTCAGTAAACGCACTACCACTGAAAAACTTCATGAAGCCTTTGCGAAGTTTGGAGAAGTTGCTCATGGTCTGTCCATTCGCATTGCAAGTTTGATTTGTGCTTAGACCTGAATTTTTTCCATCTTTTAACCTGTTGTTTTCTGTTCTGTTTGTAGCAAGAGTTGTAACTGATCGAGTGACTGGATATTCTAAAGGATTTGGCTTTGTTAAATATGCCACTTTGGAGGATGCTGCTAAAGGCATAGAGGGCATGGATGGCAAGGTGAGTTGGGATCACTTTCCAGTTTTCTCATTTGAGACGTTGAAGTTTATTTGGTGTGTATGTAGTGTTAATCCACATTTACATAAGATCCCTGGATCTTGCATTCAATGTTTTTCGCATGTGTTAGCATAGAGGCTAAGGTCGTTGACTACCCTTCTAATAGACAACTAAGAAATGAGAAAGAAAATGAATAAGAATATGACTTCAATGTGTTAATTGGCGTATCACAGAATAAATATAAGATTCCTGAATATGGTGTGTGGTGTTATGTTATCCACATATGCGTACAAGATAGTATTCTAAAGAATTTGATTGGCAACATAGAGAACATATCCTTACAGCACTCAGCTGTTGATTATGATTCATGCTTTGCATATTTTGGATCTTATGGTACCTGGCTCGGCTGATAGATCTGCTGGTTACATGGGAGTGCAGTTTCTCCTTTATTCTCATGTTCAATGGTGTTTGCTTTATGTAGTCCTTTCAGACAATAATTATTTTTGGTAATTCATTGATTTATTTGTAGGATTTTCTGATATAATTTGAATCCTTTCATTTGAGCAACATGGTGGTGAGAGTATTTTTTTTTTCATTACCTTTTTCTTGGTGGGGCATTGAGCATTTAGTCGGGTTCATTTTTTCTTTTGCTGTTGCATTGTAATGGATGTTTAAGTTTAACCGCTTAAACTGGCCTGGTTAGTGGTCACTCACGAATATAAGAAAATAAACACGTGACTTTACAATGTTGAAGAAAAACATTAACTATTGGACTTTAACAATTCTTTGAATCTTGTAAAGGGTACCACTTGGAAACATTATTGTTACTATTATCATCAAGTTTTAAAATGATTGCCAATATGTGGCTAAATGGTTGGTTGGTGAATCTTTTATAGAAAGTACAATCTTGACAATGCAAAATGGTTGTATTGAACAAACCGATCTAGGTTTCTTTTAAATCGATTTTGTCAAACTTTCCTAGAACTTCCCTTGGTTTGCTTCATTGTCATTTTTGACATTTTAGTAGAATACTCCCATTATGAGCCTCGTTCAATTGATTTGATTCCATGTGTCTTACCTCTCTGATCCCCAATTAAAACATTTACTCTGATAAACTTTACAAATTAGAATGCCCGAAAATAAGATTTAAGAAATTGGGTTGATGATTGCTTTTGAACACGTGTTGAAATTTGATTACTTATAATTGAATGATGATGAGTCTCTTTTTGTTTTCCCTCTTAATGTTATCCAGTTCCTGGATGGTTGGGTTATATTTGCTGAGTATGCAAGGCCAAGACCACCTCCTGAGAACAACAATGGTTTTGCCCCATATGGCCGTCGAGATTAACGATGAAGGATGCTTCCATATCCATTGAACTCTCTTGATCTGCAAAATGGCACTTAACTTTATATACATGATTTAGGTTATTTCTTCATTGGAGGTTCGTTGTTCTCTTCCGTTGCTGATAACTTTTTTGTTCTGCGGAACTGTAGATTAGCCGCAGGTATTGAGTTGAGAATATCTGAATTGAGACAGTAGCATTTTTACAGGTACTACATGTATGATCTACTTTTCAACATATTTAGTCAAAACTATTTTATTTATATATATGTTTCGCCAATAAACATACTTTGCATTGCGTATTTGTTACTGATAAGCTTAGAGTTTGTTCTTGTCGTGTATGCTTTTGGATTAATTTCTAGTTAACCACACATTGACC

mRNA sequence

TTTGCGTTAAGAAGAAGAGTCCTCCTTCCTCAAAACGAAAAAGAAAAAAAATCTGTTTTTCCTCGCCTCTTCCAGAAAGGGTTTAGTACCTTAAACCCCAATCCTATGGCTCTCAGATCGGCGGCGGCGTCGGCTCCTCGGAGTTTGAGGAAACTCATTACACGTACCTCAACTCGTACTCTACCGATAATCTCCCAGGCTATTCATGATTGGGAGAAACCGGAGCCCAGCACCATTACTTTCCTTTCTGGCCGCCTGTTCTCCAGCGCTTCTACTTCTAGTTTCATTCCTCCGCCGCTCCCTCCCTCGGCTCAGCAGCGTGAGGTGGCTGAGCCCAGTACTAACCTTTTCGTTTCCGGCCTCAGTAAACGCACTACCACTGAAAAACTTCATGAAGCCTTTGCGAAGTTTGGAGAAGTTGCTCATGCAAGAGTTGTAACTGATCGAGTGACTGGATATTCTAAAGGATTTGGCTTTGTTAAATATGCCACTTTGGAGGATGCTGCTAAAGGCATAGAGGGCATGGATGGCAAGTTCCTGGATGGTTGGGTTATATTTGCTGAGTATGCAAGGCCAAGACCACCTCCTGAGAACAACAATGGTTTTGCCCCATATGGCCGTCGAGATTAACGATGAAGGATGCTTCCATATCCATTGAACTCTCTTGATCTGCAAAATGGCACTTAACTTTATATACATGATTTAGGTTATTTCTTCATTGGAGGTTCGTTGTTCTCTTCCGTTGCTGATAACTTTTTTGTTCTGCGGAACTGTAGATTAGCCGCAGGTATTGAGTTGAGAATATCTGAATTGAGACAGTAGCATTTTTACAGGTACTACATGTATGATCTACTTTTCAACATATTTAGTCAAAACTATTTTATTTATATATATGTTTCGCCAATAAACATACTTTGCATTGCGTATTTGTTACTGATAAGCTTAGAGTTTGTTCTTGTCGTGTATGCTTTTGGATTAATTTCTAGTTAACCACACATTGACC

Coding sequence (CDS)

ATGGCTCTCAGATCGGCGGCGGCGTCGGCTCCTCGGAGTTTGAGGAAACTCATTACACGTACCTCAACTCGTACTCTACCGATAATCTCCCAGGCTATTCATGATTGGGAGAAACCGGAGCCCAGCACCATTACTTTCCTTTCTGGCCGCCTGTTCTCCAGCGCTTCTACTTCTAGTTTCATTCCTCCGCCGCTCCCTCCCTCGGCTCAGCAGCGTGAGGTGGCTGAGCCCAGTACTAACCTTTTCGTTTCCGGCCTCAGTAAACGCACTACCACTGAAAAACTTCATGAAGCCTTTGCGAAGTTTGGAGAAGTTGCTCATGCAAGAGTTGTAACTGATCGAGTGACTGGATATTCTAAAGGATTTGGCTTTGTTAAATATGCCACTTTGGAGGATGCTGCTAAAGGCATAGAGGGCATGGATGGCAAGTTCCTGGATGGTTGGGTTATATTTGCTGAGTATGCAAGGCCAAGACCACCTCCTGAGAACAACAATGGTTTTGCCCCATATGGCCGTCGAGATTAA

Protein sequence

MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSFIPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSKGFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRRD*

Homology

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BLAST of CSPI03G23000 vs. ExPASy Swiss-Prot
Match: Q9FIU6 (Organelle RRM domain-containing protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ORRM2 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 5.0e-40
Identity = 94/151 (62.25%), Postives = 107/151 (70.86%), Query Frame = 0


Query: 26  LPIISQAIHDWEKPEPSTITFLSGRLF-SSASTSSFIPPPLPPSAQQREVAEPSTNLFVS 85
           LP IS+A+      E ++      RLF S+AS  SF+ P        R  AEPSTNLFVS
Sbjct: 7   LPAISRAV-----TEVASAPVGLRRLFCSNASRFSFLSPQAESQTPARPQAEPSTNLFVS 66

Query: 86  GLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSKGFGFVKYATLEDAAKGIEGMDGKF 145
           GLSKRTT+E L  AFA+FGEVA A+VVTDRV+GYSKGFGFV+YATLED+AKGI GMDGKF
Sbjct: 67  GLSKRTTSEGLRTAFAQFGEVADAKVVTDRVSGYSKGFGFVRYATLEDSAKGIAGMDGKF 126

Query: 146 LDGWVIFAEYARPRP-----PPENNNGFAPY 171
           LDGWVIFAEYARPR       P+NN    PY
Sbjct: 127 LDGWVIFAEYARPREQSQSYQPQNNMSRPPY 152

[Close]

BLAST of CSPI03G23000 vs. ExPASy Swiss-Prot
Match: Q9FX45 (Organelle RRM domain-containing protein 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ORRM6 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 7.0e-18
Identity = 53/113 (46.90%), Postives = 66/113 (58.41%), Query Frame = 0


Query: 47  LSGRLFSSA-STSSFIPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEV 106
           + G L SS  ST S   PP   S        P T L+VSGLS RTT + L + F +FG +
Sbjct: 51  IGGVLISSCLSTDSSSSPPSSSSG-------PKTKLYVSGLSFRTTEDTLRDTFEQFGNL 110

Query: 107 AHARVVTDRVTGYSKGFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPR 159
            H  +V D+V    KGF F++Y T E+A K I+GM GKFLDG VIF E A+ R
Sbjct: 111 IHMNMVMDKVANRPKGFAFLRYETEEEAMKAIQGMHGKFLDGRVIFVEEAKTR 156

[Close]

BLAST of CSPI03G23000 vs. ExPASy Swiss-Prot
Match: Q9FFZ6 (Small RNA-binding protein 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=S-RBP11 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-13
Identity = 41/98 (41.84%), Postives = 62/98 (63.27%), Query Frame = 0


Query: 70  QQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSKGFGFVKYAT 129
           Q+R VA   + LF+ GLS  TT + L EAF+K G+V  A++V DRV+  SKGFGFV +A+
Sbjct: 28  QRRGVA---SKLFIGGLSFCTTEQGLSEAFSKCGQVVEAQIVMDRVSDRSKGFGFVTFAS 87

Query: 130 LEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGF 168
            ++A K +   +G+ L+G  IF +YA+ +       G+
Sbjct: 88  ADEAQKALMEFNGQQLNGRTIFVDYAKAKQSLGGGGGY 122

[Close]

BLAST of CSPI03G23000 vs. ExPASy Swiss-Prot
Match: Q9FNR1 (Glycine-rich RNA-binding protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBG3 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.8e-13
Identity = 38/92 (41.30%), Postives = 53/92 (57.61%), Query Frame = 0


Query: 67  PSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSKGFGFVK 126
           PS  Q      S+ LF+ G++     + L EAF K+GEV   RV+ DR TG S+GFGFV 
Sbjct: 28  PSLFQAIRCMSSSKLFIGGMAYSMDEDSLREAFTKYGEVVDTRVILDRETGRSRGFGFVT 87

Query: 127 YATLEDAAKGIEGMDGKFLDGWVIFAEYARPR 159
           + + E A+  I+ +DG+ L G V+   YA  R
Sbjct: 88  FTSSEAASSAIQALDGRDLHGRVVKVNYANDR 119

[Close]

BLAST of CSPI03G23000 vs. ExPASy Swiss-Prot
Match: Q99070 (Glycine-rich RNA-binding protein 2 OS=Sorghum bicolor OX=4558 GN=GRP2 PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.4e-12
Identity = 39/97 (40.21%), Postives = 54/97 (55.67%), Query Frame = 0


Query: 75  AEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSKGFGFVKYATLEDAA 134
           A+     FV GL+  T  E L +AFA FG+V  ++V+TDR TG S+GFGFV +++ +   
Sbjct: 4   ADVEYRCFVGGLAWATNNETLEQAFANFGQVIDSKVITDRETGRSRGFGFVTFSSEQSML 63

Query: 135 KGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYG 172
             IE M+GK LDG  I    A+ R       G+   G
Sbjct: 64  DAIENMNGKELDGRNITVNQAQSRGGGGGGGGYGGGG 100

[Close]

BLAST of CSPI03G23000 vs. ExPASy TrEMBL
Match: A0A0A0LD10 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G403980 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 7.6e-92
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60
           MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF
Sbjct: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60

Query: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120
           IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK
Sbjct: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120

Query: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRRD 175
           GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRRD
Sbjct: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRRD 174

[Close]

BLAST of CSPI03G23000 vs. ExPASy TrEMBL
Match: A0A1S3C066 (glycine-rich RNA-binding protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103494967 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.1e-85
Identity = 162/173 (93.64%), Postives = 167/173 (96.53%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60
           MALRSAAASAPRSLR+LIT TSTRTLPIISQAIHDWEKP PST +FL GRLFSSASTSSF
Sbjct: 1   MALRSAAASAPRSLRQLITGTSTRTLPIISQAIHDWEKPVPSTNSFLFGRLFSSASTSSF 60

Query: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120
           +PPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAF+KFGEV HARVVTDR TGYSK
Sbjct: 61  VPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFSKFGEVVHARVVTDRATGYSK 120

Query: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRR 174
           GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGR+
Sbjct: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRQ 173

[Close]

BLAST of CSPI03G23000 vs. ExPASy TrEMBL
Match: A0A5D3DR24 (Glycine-rich RNA-binding protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold668G00050 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.0e-85
Identity = 162/172 (94.19%), Postives = 166/172 (96.51%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60
           MALRSAAASAPRSLR+LIT TSTRTLPIISQAIHDWEKP PST +FL GRLFSSASTSSF
Sbjct: 1   MALRSAAASAPRSLRQLITGTSTRTLPIISQAIHDWEKPVPSTNSFLFGRLFSSASTSSF 60

Query: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120
           +PPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAF+KFGEV HARVVTDR TGYSK
Sbjct: 61  VPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFSKFGEVVHARVVTDRATGYSK 120

Query: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGR 173
           GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGR
Sbjct: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGR 172

[Close]

BLAST of CSPI03G23000 vs. ExPASy TrEMBL
Match: A0A6J1INZ4 (organelle RRM domain-containing protein 2, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111476943 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.9e-75
Identity = 149/173 (86.13%), Postives = 156/173 (90.17%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60
           MALRSAAA+APRSLR+LIT TS RTLP+  QAIHD EKP P+T  F+SGRLFSSASTSSF
Sbjct: 1   MALRSAAAAAPRSLRRLITGTSARTLPMFLQAIHDCEKPTPNTNAFVSGRLFSSASTSSF 60

Query: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120
           IPPPLPPSA QRE  EPSTNLFVSGLSKRTTTEKLHE FAKFGEV HARVVTDRVTGYSK
Sbjct: 61  IPPPLPPSAHQREQPEPSTNLFVSGLSKRTTTEKLHETFAKFGEVVHARVVTDRVTGYSK 120

Query: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRR 174
           GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPE +N   PYGR+
Sbjct: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPE-HNAVPPYGRQ 172

[Close]

BLAST of CSPI03G23000 vs. ExPASy TrEMBL
Match: A0A6J1FB41 (organelle RRM domain-containing protein 2, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111443707 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.4e-74
Identity = 151/173 (87.28%), Postives = 157/173 (90.75%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKP-EPSTITFLSGRLFSSASTSS 60
           MALRSAAA+APRSLR+LIT TS RTLPI  QAIHD EKP  P+T  F+SGRLFSSASTSS
Sbjct: 1   MALRSAAAAAPRSLRRLITGTSARTLPIFLQAIHDCEKPTPPNTNAFVSGRLFSSASTSS 60

Query: 61  FIPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYS 120
           FIPPPLPPSAQQRE  EPSTNLFVSGLSKRTTTEKL+EAFAKFGEV HARVVTDRVTGYS
Sbjct: 61  FIPPPLPPSAQQREQPEPSTNLFVSGLSKRTTTEKLNEAFAKFGEVVHARVVTDRVTGYS 120

Query: 121 KGFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGR 173
           KGFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPE +N   PYGR
Sbjct: 121 KGFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPE-HNAVPPYGR 172

[Close]

BLAST of CSPI03G23000 vs. NCBI nr
Match: XP_004151968.1 (organelle RRM domain-containing protein 2, mitochondrial [Cucumis sativus] >KGN57946.1 hypothetical protein Csa_009766 [Cucumis sativus])

HSP 1 Score: 346.3 bits (887), Expect = 1.6e-91
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60
           MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF
Sbjct: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60

Query: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120
           IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK
Sbjct: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120

Query: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRRD 175
           GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRRD
Sbjct: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRRD 174

[Close]

BLAST of CSPI03G23000 vs. NCBI nr
Match: XP_008454580.1 (PREDICTED: glycine-rich RNA-binding protein 4, mitochondrial [Cucumis melo])

HSP 1 Score: 324.3 bits (830), Expect = 6.4e-85
Identity = 162/173 (93.64%), Postives = 167/173 (96.53%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60
           MALRSAAASAPRSLR+LIT TSTRTLPIISQAIHDWEKP PST +FL GRLFSSASTSSF
Sbjct: 1   MALRSAAASAPRSLRQLITGTSTRTLPIISQAIHDWEKPVPSTNSFLFGRLFSSASTSSF 60

Query: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120
           +PPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAF+KFGEV HARVVTDR TGYSK
Sbjct: 61  VPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFSKFGEVVHARVVTDRATGYSK 120

Query: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRR 174
           GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGR+
Sbjct: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRQ 173

[Close]

BLAST of CSPI03G23000 vs. NCBI nr
Match: KAA0063485.1 (glycine-rich RNA-binding protein 4 [Cucumis melo var. makuwa] >TYK25992.1 glycine-rich RNA-binding protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 323.9 bits (829), Expect = 8.3e-85
Identity = 162/172 (94.19%), Postives = 166/172 (96.51%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60
           MALRSAAASAPRSLR+LIT TSTRTLPIISQAIHDWEKP PST +FL GRLFSSASTSSF
Sbjct: 1   MALRSAAASAPRSLRQLITGTSTRTLPIISQAIHDWEKPVPSTNSFLFGRLFSSASTSSF 60

Query: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120
           +PPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAF+KFGEV HARVVTDR TGYSK
Sbjct: 61  VPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFSKFGEVVHARVVTDRATGYSK 120

Query: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGR 173
           GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGR
Sbjct: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGR 172

[Close]

BLAST of CSPI03G23000 vs. NCBI nr
Match: XP_038896843.1 (organelle RRM domain-containing protein 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 308.9 bits (790), Expect = 2.8e-80
Identity = 158/173 (91.33%), Postives = 162/173 (93.64%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60
           MALRSAAA+APRSLR+LIT TSTRTLPIISQ IHDWEK EPST  FLSGRLFSSASTSSF
Sbjct: 1   MALRSAAAAAPRSLRRLITGTSTRTLPIISQTIHDWEKLEPSTNAFLSGRLFSSASTSSF 60

Query: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120
           IPPPLPPSAQQRE  EPSTNLFVSGLSKRTTTEKLHEAFAKFGEV HARVVTDRVTGYSK
Sbjct: 61  IPPPLPPSAQQREQPEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVVHARVVTDRVTGYSK 120

Query: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRR 174
           GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPE NNGF P+G +
Sbjct: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPE-NNGFRPHGHQ 172

[Close]

BLAST of CSPI03G23000 vs. NCBI nr
Match: XP_022976594.1 (organelle RRM domain-containing protein 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 291.2 bits (744), Expect = 6.0e-75
Identity = 149/173 (86.13%), Postives = 156/173 (90.17%), Query Frame = 0


Query: 1   MALRSAAASAPRSLRKLITRTSTRTLPIISQAIHDWEKPEPSTITFLSGRLFSSASTSSF 60
           MALRSAAA+APRSLR+LIT TS RTLP+  QAIHD EKP P+T  F+SGRLFSSASTSSF
Sbjct: 1   MALRSAAAAAPRSLRRLITGTSARTLPMFLQAIHDCEKPTPNTNAFVSGRLFSSASTSSF 60

Query: 61  IPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSK 120
           IPPPLPPSA QRE  EPSTNLFVSGLSKRTTTEKLHE FAKFGEV HARVVTDRVTGYSK
Sbjct: 61  IPPPLPPSAHQREQPEPSTNLFVSGLSKRTTTEKLHETFAKFGEVVHARVVTDRVTGYSK 120

Query: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGFAPYGRR 174
           GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPE +N   PYGR+
Sbjct: 121 GFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPE-HNAVPPYGRQ 172

[Close]

BLAST of CSPI03G23000 vs. TAIR 10
Match: AT5G54580.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 165.6 bits (418), Expect = 3.5e-41
Identity = 94/151 (62.25%), Postives = 107/151 (70.86%), Query Frame = 0


Query: 26  LPIISQAIHDWEKPEPSTITFLSGRLF-SSASTSSFIPPPLPPSAQQREVAEPSTNLFVS 85
           LP IS+A+      E ++      RLF S+AS  SF+ P        R  AEPSTNLFVS
Sbjct: 7   LPAISRAV-----TEVASAPVGLRRLFCSNASRFSFLSPQAESQTPARPQAEPSTNLFVS 66

Query: 86  GLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSKGFGFVKYATLEDAAKGIEGMDGKF 145
           GLSKRTT+E L  AFA+FGEVA A+VVTDRV+GYSKGFGFV+YATLED+AKGI GMDGKF
Sbjct: 67  GLSKRTTSEGLRTAFAQFGEVADAKVVTDRVSGYSKGFGFVRYATLEDSAKGIAGMDGKF 126

Query: 146 LDGWVIFAEYARPRP-----PPENNNGFAPY 171
           LDGWVIFAEYARPR       P+NN    PY
Sbjct: 127 LDGWVIFAEYARPREQSQSYQPQNNMSRPPY 152

[Close]

BLAST of CSPI03G23000 vs. TAIR 10
Match: AT2G37510.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 111.3 bits (277), Expect = 7.9e-25
Identity = 54/83 (65.06%), Postives = 63/83 (75.90%), Query Frame = 0


Query: 78  STNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSKGFGFVKYATLEDAAKGI 137
           S  LFVSGLS+ TT EKL +AFA FG++  ARV+TDR +G SKGFGFV YAT+EDA K  
Sbjct: 33  SPRLFVSGLSRLTTNEKLQDAFASFGQLVDARVITDRDSGRSKGFGFVTYATIEDAEKAK 92

Query: 138 EGMDGKFLDGWVIFAEYARPRPP 161
             M+ KFLDGWVIF + ARPR P
Sbjct: 93  AEMNAKFLDGWVIFVDPARPREP 115

[Close]

BLAST of CSPI03G23000 vs. TAIR 10
Match: AT1G73530.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 92.0 bits (227), Expect = 4.9e-19
Identity = 53/113 (46.90%), Postives = 66/113 (58.41%), Query Frame = 0


Query: 47  LSGRLFSSA-STSSFIPPPLPPSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEV 106
           + G L SS  ST S   PP   S        P T L+VSGLS RTT + L + F +FG +
Sbjct: 51  IGGVLISSCLSTDSSSSPPSSSSG-------PKTKLYVSGLSFRTTEDTLRDTFEQFGNL 110

Query: 107 AHARVVTDRVTGYSKGFGFVKYATLEDAAKGIEGMDGKFLDGWVIFAEYARPR 159
            H  +V D+V    KGF F++Y T E+A K I+GM GKFLDG VIF E A+ R
Sbjct: 111 IHMNMVMDKVANRPKGFAFLRYETEEEAMKAIQGMHGKFLDGRVIFVEEAKTR 156

[Close]

BLAST of CSPI03G23000 vs. TAIR 10
Match: AT5G06210.1 (RNA binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 77.8 bits (190), Expect = 9.7e-15
Identity = 41/98 (41.84%), Postives = 62/98 (63.27%), Query Frame = 0


Query: 70  QQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSKGFGFVKYAT 129
           Q+R VA   + LF+ GLS  TT + L EAF+K G+V  A++V DRV+  SKGFGFV +A+
Sbjct: 28  QRRGVA---SKLFIGGLSFCTTEQGLSEAFSKCGQVVEAQIVMDRVSDRSKGFGFVTFAS 87

Query: 130 LEDAAKGIEGMDGKFLDGWVIFAEYARPRPPPENNNGF 168
            ++A K +   +G+ L+G  IF +YA+ +       G+
Sbjct: 88  ADEAQKALMEFNGQQLNGRTIFVDYAKAKQSLGGGGGY 122

[Close]

BLAST of CSPI03G23000 vs. TAIR 10
Match: AT5G61030.1 (glycine-rich RNA-binding protein 3 )

HSP 1 Score: 75.1 bits (183), Expect = 6.3e-14
Identity = 38/92 (41.30%), Postives = 53/92 (57.61%), Query Frame = 0


Query: 67  PSAQQREVAEPSTNLFVSGLSKRTTTEKLHEAFAKFGEVAHARVVTDRVTGYSKGFGFVK 126
           PS  Q      S+ LF+ G++     + L EAF K+GEV   RV+ DR TG S+GFGFV 
Sbjct: 28  PSLFQAIRCMSSSKLFIGGMAYSMDEDSLREAFTKYGEVVDTRVILDRETGRSRGFGFVT 87

Query: 127 YATLEDAAKGIEGMDGKFLDGWVIFAEYARPR 159
           + + E A+  I+ +DG+ L G V+   YA  R
Sbjct: 88  FTSSEAASSAIQALDGRDLHGRVVKVNYANDR 119

The following BLAST results are available for this feature:

BLAST of CSPI03G23000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FIU6	5.0e-40	62.25	Organelle RRM domain-containing protein 2, mitochondrial OS=Arabidopsis thaliana...	[more]
Q9FX45	7.0e-18	46.90	Organelle RRM domain-containing protein 6, chloroplastic OS=Arabidopsis thaliana...	[more]
Q9FFZ6	1.4e-13	41.84	Small RNA-binding protein 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=S...	[more]
Q9FNR1	8.8e-13	41.30	Glycine-rich RNA-binding protein 3, mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q99070	4.4e-12	40.21	Glycine-rich RNA-binding protein 2 OS=Sorghum bicolor OX=4558 GN=GRP2 PE=2 SV=1	[more]

BLAST of CSPI03G23000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LD10	7.6e-92	100.00	RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G403980 PE=4 SV...	[more]
A0A1S3C066	3.1e-85	93.64	glycine-rich RNA-binding protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5D3DR24	4.0e-85	94.19	Glycine-rich RNA-binding protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1INZ4	2.9e-75	86.13	organelle RRM domain-containing protein 2, mitochondrial-like OS=Cucurbita maxim...	[more]
A0A6J1FB41	2.4e-74	87.28	organelle RRM domain-containing protein 2, mitochondrial-like OS=Cucurbita mosch...	[more]

BLAST of CSPI03G23000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151968.1	1.6e-91	100.00	organelle RRM domain-containing protein 2, mitochondrial [Cucumis sativus] >KGN5...	[more]
XP_008454580.1	6.4e-85	93.64	PREDICTED: glycine-rich RNA-binding protein 4, mitochondrial [Cucumis melo]	[more]
KAA0063485.1	8.3e-85	94.19	glycine-rich RNA-binding protein 4 [Cucumis melo var. makuwa] >TYK25992.1 glycin...	[more]
XP_038896843.1	2.8e-80	91.33	organelle RRM domain-containing protein 2, mitochondrial [Benincasa hispida]	[more]
XP_022976594.1	6.0e-75	86.13	organelle RRM domain-containing protein 2, mitochondrial-like [Cucurbita maxima]	[more]

BLAST of CSPI03G23000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54580.1	3.5e-41	62.25	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT2G37510.1	7.9e-25	65.06	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT1G73530.1	4.9e-19	46.90	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT5G06210.1	9.7e-15	41.84	RNA binding (RRM/RBD/RNP motifs) family protein	[more]
AT5G61030.1	6.3e-14	41.30	glycine-rich RNA-binding protein 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 80..153 e-value: 8.9E-24 score: 95.0
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 81..147 e-value: 2.9E-19 score: 68.6
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 79..157 score: 18.410337
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 66..174 e-value: 2.2E-28 score: 101.1
None	No IPR available	PANTHER	PTHR15241:SF280	GLYCINE-RICH RNA-BINDING PROTEIN 3, MITOCHONDRIAL-LIKE	coord: 50..164
None	No IPR available	PANTHER	PTHR15241	TRANSFORMER-2-RELATED	coord: 50..164
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 60..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G23000.1	CSPI03G23000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0080156	mitochondrial mRNA modification
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003676	nucleic acid binding