CSPI03G22800 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G22800
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCDT1-like protein b
LocationChr3: 19470343 .. 19470717 (+)
RNA-Seq ExpressionCSPI03G22800
SyntenyCSPI03G22800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTACTCTTAGTCTGTAATAATGGCGGACGCTTCAAACAAGATTTCTTCTCGTTCATCAACTTTACCCTTCAAATCGAAGAAAAATTTGGGCTCCAACTCCTCATCATTGCTCAAGGATCCGCAACCTCTAAGCTCCAGGACGCCGGAGAAGCCGGTGGAGCGAAATAGGAAACGGAAGGTTGCGCTTTCCATTAAAGAGGTTAAACAAGCTGCTCAGAGCGTCCGTGAATCTAATCGTCAGTTGCATCATGATCTGACCACTAGAGGGAGTAAATTAGTTAGAAGACAGATGGATTCGTGGTCAAATGAAAGTCAATCTAGTAGATCCAAGACTTGTGTTGATAATAAGTCAAATAAATTGCCTGAAAAGTAA

mRNA sequence

CTTTACTCTTAGTCTGTAATAATGGCGGACGCTTCAAACAAGATTTCTTCTCGTTCATCAACTTTACCCTTCAAATCGAAGAAAAATTTGGGCTCCAACTCCTCATCATTGCTCAAGGATCCGCAACCTCTAAGCTCCAGGACGCCGGAGAAGCCGGTGGAGCGAAATAGGAAACGGAAGGTTGCGCTTTCCATTAAAGAGGTTAAACAAGCTGCTCAGAGCGTCCGTGAATCTAATCGTCAGTTGCATCATGATCTGACCACTAGAGGGAGTAAATTAGTTAGAAGACAGATGGATTCGTGGTCAAATGAAAGTCAATCTAGTAGATCCAAGACTTGTGTTGATAATAAGTCAAATAAATTGCCTGAAAAGTAA

Coding sequence (CDS)

ATGGCGGACGCTTCAAACAAGATTTCTTCTCGTTCATCAACTTTACCCTTCAAATCGAAGAAAAATTTGGGCTCCAACTCCTCATCATTGCTCAAGGATCCGCAACCTCTAAGCTCCAGGACGCCGGAGAAGCCGGTGGAGCGAAATAGGAAACGGAAGGTTGCGCTTTCCATTAAAGAGGTTAAACAAGCTGCTCAGAGCGTCCGTGAATCTAATCGTCAGTTGCATCATGATCTGACCACTAGAGGGAGTAAATTAGTTAGAAGACAGATGGATTCGTGGTCAAATGAAAGTCAATCTAGTAGATCCAAGACTTGTGTTGATAATAAGTCAAATAAATTGCCTGAAAAGTAA

Protein sequence

MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKRKVALSIKEVKQAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK*
Homology
BLAST of CSPI03G22800 vs. ExPASy Swiss-Prot
Match: Q9SJW9 (CDT1-like protein a, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CDT1A PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 8.6e-04
Identity = 43/115 (37.39%), Postives = 59/115 (51.30%), Query Frame = 0

Query: 12  SSTLPFKSKKNL-----GSNSSSLLKDPQPLSSRTPEKPVE----RNRKRKVALSIKEVK 71
           S ++PFKSKK L      S S +   +P  ++  TPEKP+E    R+R R VALS+KE++
Sbjct: 7   SRSIPFKSKKRLVMDSPSSKSQTGNPNPSSVALPTPEKPLENMLSRSRNRSVALSVKEIR 66

Query: 72  QAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 118
           QAA S R S   +        S    R     S+ S S R  +  + +  KLPEK
Sbjct: 67  QAAGSRRRSEDPV-------ASSAKSRLFFDSSSSSPSKRKSSNKNAEKEKLPEK 114

BLAST of CSPI03G22800 vs. ExPASy TrEMBL
Match: A0A0A0L7I7 (CDT1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G394670 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.7e-50
Identity = 115/117 (98.29%), Postives = 115/117 (98.29%), Query Frame = 0

Query: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKRKVALSIKE 60
           MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQ LSSRTPEKPVERNRKRKVALSIKE
Sbjct: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQSLSSRTPEKPVERNRKRKVALSIKE 60

Query: 61  VKQAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 118
           VKQAAQSV ESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK
Sbjct: 61  VKQAAQSVHESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 117

BLAST of CSPI03G22800 vs. ExPASy TrEMBL
Match: A0A5A7T8X4 (CDT1-like protein a OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00930 PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 9.1e-41
Identity = 101/117 (86.32%), Postives = 105/117 (89.74%), Query Frame = 0

Query: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKRKVALSIKE 60
           MADAS+ ISS SSTLPFKSKKNLGSN  SLLKDPQ LSS+TPEKP ER RKRKVALSIKE
Sbjct: 1   MADASHNISSPSSTLPFKSKKNLGSNFKSLLKDPQSLSSKTPEKPAERTRKRKVALSIKE 60

Query: 61  VKQAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 118
           VK+AAQSV ESNRQL HDLTT+GSKLVRRQMDS SNES SSRSKTCVD KSNKLPEK
Sbjct: 61  VKRAAQSVNESNRQLPHDLTTKGSKLVRRQMDSSSNESLSSRSKTCVDKKSNKLPEK 117

BLAST of CSPI03G22800 vs. ExPASy TrEMBL
Match: A0A1S3BGW9 (CDT1-like protein a, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489888 PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 9.1e-41
Identity = 101/117 (86.32%), Postives = 105/117 (89.74%), Query Frame = 0

Query: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKRKVALSIKE 60
           MADAS+ ISS SSTLPFKSKKNLGSN  SLLKDPQ LSS+TPEKP ER RKRKVALSIKE
Sbjct: 1   MADASHNISSPSSTLPFKSKKNLGSNFKSLLKDPQSLSSKTPEKPAERTRKRKVALSIKE 60

Query: 61  VKQAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 118
           VK+AAQSV ESNRQL HDLTT+GSKLVRRQMDS SNES SSRSKTCVD KSNKLPEK
Sbjct: 61  VKRAAQSVNESNRQLPHDLTTKGSKLVRRQMDSSSNESLSSRSKTCVDKKSNKLPEK 117

BLAST of CSPI03G22800 vs. ExPASy TrEMBL
Match: A0A0A0LD77 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G842990 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.7e-32
Identity = 95/121 (78.51%), Postives = 103/121 (85.12%), Query Frame = 0

Query: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKR-KVALSIK 60
           MA ASNKISS SSTLPFKS KN+ SNS+SLLKDPQ LSS+TPEKP ER RKR KVALSI+
Sbjct: 1   MAGASNKISSPSSTLPFKSNKNVSSNSTSLLKDPQSLSSKTPEKPAERTRKRKKVALSIE 60

Query: 61  EVKQAAQSVRESNRQ-LHHDLTTRGSKLVRRQMDSWSNESQSSRS-KTCVDNK-SNKLPE 118
           EV++AAQSV ESN Q L HDLTT GSK+VRR+MDS SNESQSS S  TCVDNK SNKLPE
Sbjct: 61  EVERAAQSVHESNSQLLPHDLTTEGSKVVRRRMDSRSNESQSSSSDSTCVDNKYSNKLPE 120

BLAST of CSPI03G22800 vs. ExPASy TrEMBL
Match: A0A6J1HQP8 (CDT1-like protein b OS=Cucurbita maxima OX=3661 GN=LOC111466932 PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 6.1e-29
Identity = 87/118 (73.73%), Postives = 94/118 (79.66%), Query Frame = 0

Query: 3   DASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVE---RNRKRKVALSIK 62
           DAS KISS SS+LPFKSKK L SNS SLL+D Q LSS+TPEK  E   R R RKVALSIK
Sbjct: 2   DASEKISSPSSSLPFKSKKTLRSNSKSLLQDQQSLSSKTPEKSAELSTRTRSRKVALSIK 61

Query: 63  EVKQAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 118
           EV++AA+SVRESNRQL  DLTT GSK VRRQ+DSWSNES SSR KT VD K  KLPEK
Sbjct: 62  EVRRAAESVRESNRQLPRDLTTEGSKSVRRQIDSWSNESPSSRFKTSVD-KYKKLPEK 118

BLAST of CSPI03G22800 vs. NCBI nr
Match: XP_004151978.3 (CDT1-like protein a, chloroplastic [Cucumis sativus] >KAE8650673.1 hypothetical protein Csa_009499 [Cucumis sativus])

HSP 1 Score: 208.4 bits (529), Expect = 3.4e-50
Identity = 115/117 (98.29%), Postives = 115/117 (98.29%), Query Frame = 0

Query: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKRKVALSIKE 60
           MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQ LSSRTPEKPVERNRKRKVALSIKE
Sbjct: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQSLSSRTPEKPVERNRKRKVALSIKE 60

Query: 61  VKQAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 118
           VKQAAQSV ESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK
Sbjct: 61  VKQAAQSVHESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 117

BLAST of CSPI03G22800 vs. NCBI nr
Match: XP_031737520.1 (CDT1-like protein a, chloroplastic [Cucumis sativus] >KGN57983.2 hypothetical protein Csa_010006 [Cucumis sativus])

HSP 1 Score: 204.5 bits (519), Expect = 5.0e-49
Identity = 113/117 (96.58%), Postives = 113/117 (96.58%), Query Frame = 0

Query: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKRKVALSIKE 60
           MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQ LSSRTPEKPVERNRKRKVALSIKE
Sbjct: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQSLSSRTPEKPVERNRKRKVALSIKE 60

Query: 61  VKQAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 118
           VKQAAQSV ESNRQLHHDLTTRGSKLVRRQMDSWS ESQSSRSKTCVDNKSNK PEK
Sbjct: 61  VKQAAQSVHESNRQLHHDLTTRGSKLVRRQMDSWSTESQSSRSKTCVDNKSNKFPEK 117

BLAST of CSPI03G22800 vs. NCBI nr
Match: XP_008447443.1 (PREDICTED: CDT1-like protein a, chloroplastic [Cucumis melo] >KAA0038091.1 CDT1-like protein a [Cucumis melo var. makuwa] >TYK20516.1 CDT1-like protein a [Cucumis melo var. makuwa])

HSP 1 Score: 176.0 bits (445), Expect = 1.9e-40
Identity = 101/117 (86.32%), Postives = 105/117 (89.74%), Query Frame = 0

Query: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKRKVALSIKE 60
           MADAS+ ISS SSTLPFKSKKNLGSN  SLLKDPQ LSS+TPEKP ER RKRKVALSIKE
Sbjct: 1   MADASHNISSPSSTLPFKSKKNLGSNFKSLLKDPQSLSSKTPEKPAERTRKRKVALSIKE 60

Query: 61  VKQAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 118
           VK+AAQSV ESNRQL HDLTT+GSKLVRRQMDS SNES SSRSKTCVD KSNKLPEK
Sbjct: 61  VKRAAQSVNESNRQLPHDLTTKGSKLVRRQMDSSSNESLSSRSKTCVDKKSNKLPEK 117

BLAST of CSPI03G22800 vs. NCBI nr
Match: XP_031739146.1 (CDT1-like protein a, chloroplastic [Cucumis sativus])

HSP 1 Score: 147.9 bits (372), Expect = 5.5e-32
Identity = 95/121 (78.51%), Postives = 103/121 (85.12%), Query Frame = 0

Query: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKR-KVALSIK 60
           MA ASNKISS SSTLPFKS KN+ SNS+SLLKDPQ LSS+TPEKP ER RKR KVALSI+
Sbjct: 1   MAGASNKISSPSSTLPFKSNKNVSSNSTSLLKDPQSLSSKTPEKPAERTRKRKKVALSIE 60

Query: 61  EVKQAAQSVRESNRQ-LHHDLTTRGSKLVRRQMDSWSNESQSSRS-KTCVDNK-SNKLPE 118
           EV++AAQSV ESN Q L HDLTT GSK+VRR+MDS SNESQSS S  TCVDNK SNKLPE
Sbjct: 61  EVERAAQSVHESNSQLLPHDLTTEGSKVVRRRMDSRSNESQSSSSDSTCVDNKYSNKLPE 120

BLAST of CSPI03G22800 vs. NCBI nr
Match: KAE8651274.1 (hypothetical protein Csa_001826, partial [Cucumis sativus])

HSP 1 Score: 147.9 bits (372), Expect = 5.5e-32
Identity = 95/121 (78.51%), Postives = 103/121 (85.12%), Query Frame = 0

Query: 1   MADASNKISSRSSTLPFKSKKNLGSNSSSLLKDPQPLSSRTPEKPVERNRKR-KVALSIK 60
           MA ASNKISS SSTLPFKS KN+ SNS+SLLKDPQ LSS+TPEKP ER RKR KVALSI+
Sbjct: 1   MAGASNKISSPSSTLPFKSNKNVSSNSTSLLKDPQSLSSKTPEKPAERTRKRKKVALSIE 60

Query: 61  EVKQAAQSVRESNRQ-LHHDLTTRGSKLVRRQMDSWSNESQSSRS-KTCVDNK-SNKLPE 118
           EV++AAQSV ESN Q L HDLTT GSK+VRR+MDS SNESQSS S  TCVDNK SNKLPE
Sbjct: 61  EVERAAQSVHESNSQLLPHDLTTEGSKVVRRRMDSRSNESQSSSSDSTCVDNKYSNKLPE 120

BLAST of CSPI03G22800 vs. TAIR 10
Match: AT2G31270.1 (homolog of yeast CDT1 A )

HSP 1 Score: 44.7 bits (104), Expect = 6.1e-05
Identity = 43/115 (37.39%), Postives = 59/115 (51.30%), Query Frame = 0

Query: 12  SSTLPFKSKKNL-----GSNSSSLLKDPQPLSSRTPEKPVE----RNRKRKVALSIKEVK 71
           S ++PFKSKK L      S S +   +P  ++  TPEKP+E    R+R R VALS+KE++
Sbjct: 7   SRSIPFKSKKRLVMDSPSSKSQTGNPNPSSVALPTPEKPLENMLSRSRNRSVALSVKEIR 66

Query: 72  QAAQSVRESNRQLHHDLTTRGSKLVRRQMDSWSNESQSSRSKTCVDNKSNKLPEK 118
           QAA S R S   +        S    R     S+ S S R  +  + +  KLPEK
Sbjct: 67  QAAGSRRRSEDPV-------ASSAKSRLFFDSSSSSPSKRKSSNKNAEKEKLPEK 114

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJW98.6e-0437.39CDT1-like protein a, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CDT1A PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0L7I71.7e-5098.29CDT1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G394670 PE=3 S... [more]
A0A5A7T8X49.1e-4186.32CDT1-like protein a OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G... [more]
A0A1S3BGW99.1e-4186.32CDT1-like protein a, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489888 PE=3 ... [more]
A0A0A0LD772.7e-3278.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G842990 PE=4 SV=1[more]
A0A6J1HQP86.1e-2973.73CDT1-like protein b OS=Cucurbita maxima OX=3661 GN=LOC111466932 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004151978.33.4e-5098.29CDT1-like protein a, chloroplastic [Cucumis sativus] >KAE8650673.1 hypothetical ... [more]
XP_031737520.15.0e-4996.58CDT1-like protein a, chloroplastic [Cucumis sativus] >KGN57983.2 hypothetical pr... [more]
XP_008447443.11.9e-4086.32PREDICTED: CDT1-like protein a, chloroplastic [Cucumis melo] >KAA0038091.1 CDT1-... [more]
XP_031739146.15.5e-3278.51CDT1-like protein a, chloroplastic [Cucumis sativus][more]
KAE8651274.15.5e-3278.51hypothetical protein Csa_001826, partial [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT2G31270.16.1e-0537.39homolog of yeast CDT1 A [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..58
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 86..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..117

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G22800.1CSPI03G22800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000076 DNA replication checkpoint signaling
biological_process GO:0071163 DNA replication preinitiation complex assembly
biological_process GO:0000278 mitotic cell cycle
biological_process GO:0030174 regulation of DNA-dependent DNA replication initiation
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0070182 DNA polymerase binding