CSPI03G22540 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G22540
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description11S globulin seed storage protein 2-like
LocationChr3: 19110349 .. 19112226 (-)
RNA-Seq ExpressionCSPI03G22540
SyntenyCSPI03G22540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTTAAAGTTTTATTGGCGATTCTACTATGTTTCTTTGCTTGCGAGTCTCTCGTGAACGCTCGGGACCCATCTGAGAGGCGTGGGTTTCGGTTCACCACAGAAGCTCAGCTATGCAAGCTTGACAAGATTCGGGTTAGGCCACCATCACGAAGGATCGAGTCGGAGGGAGGCATCACTGAGCTTTGGGATGAGGCTGATGAAGAGTTTCAGTGTGCTGGAGTTGGTGCCATTAGAAACATCATAAGGCCCAATTCTCTCTCTTTGCCTAAATTCCACAATGCCCCTATGCTTGTTTACATCGAGCAAGGTATATTATTCTTAACAAAATTGCTTCCAAATATGTCTTTAATCACTTAACATTAAAAATATGTTTCAAACTAATTTTAAACCTAACAAAACGCCCAAATATGCCCTAAACTTCTAGAGGTTACATGATATTGAATCATTTATATTCATATTCTTCATACTTCCAAATGGTATAACAATTAAAAATTTATTTGTGTTACAATTACATGTAAATGAATAATATATGTTACGAAAGTCAATTATTCATGTTCTAAAGCAATGGTTTACAAATGGGGTGGAACAGGTGAAGCGTTTTTCGGGATGAACTACCCAGGGTGTGCAGAGACATACGAATCACAGTCAGCTCAATCTTCAAGGTCTACAAGGCGCATGGGACGACGAATTGGCGCCGGCAGAACAGAAGAAGACCAACACCAAAAGATTCGCAGAGTTCGACGTGGTGACATGATCGTCATTCCCGCTGGTACCGTCCAATGGTGCTACAACGATGGCGGCGAAGACCTCATTGCCGTGGCCTTCCTGGATCTCAACAATGATGACAACCAACTCGACCTCCGCGTCAGGGTATTTCAATTCTCTACCTAATTTAAAACTCGCAAGAACCGGTTCTGCATTTGACCCTGACGTGCTATTTATTTTTTATAGGGATCTTTCTTGGCTGGTGGAGTTCCAAGTGAATCAAGAAGGGAAATAAGAGGATCAAAATCAGAAAATCTTGTGAACATCTTCAGTGGGTTAGATCAAGAGTTTCTTTCAGAGGCTTACAACATTCCATCAGAATTAGTGAGGAGAATGCAAGAAGAAAGGAGCAGTGGGCTCATTGTGAAGTGTGACGAAGAGATGTCCTTTTTAACTCCGGAGGAAGAAGAGGAAGAATTAAGCGAAACGTCATTTTCAAGAAGGAGAGGAGAATACTCAAATGGGATAGAAGAAACCGTATGCACTGCTAGAGTTCAACACAACATGAACACCCAAAGAGAAGCTGATCTATTCTCTAGAGAAGCTGGTAGAGTTAACATCTTGAACCAACTCAAGCTCCCTATCCTAAGATTCTTGGGCATGAGTGCTGAGAAAGGCCACCTTTTCGCGGTAAAACACTCATTTTTTAGATGTCATTTTCTCTATAATAATTAACCTAACGATTTCGAATTTTCGTAATTGTAGAATGCTCAACACAACTTGCATTGGTCAATGACGGATCACAGAATGGTGTACGTAGTAGACGGAGAGGCCGAAATTCAAATATCCGACGACTACGGAAACCAAGTATTCAATGAGAGAGTATCAAGAGGAAACATGTTTGTGATTCCTCAATTCTACCCTGCATTAGCTAGAGCTGGGCAAGAAGGATTTGAATGGGTAACTTTCAAGACATCAAATCAGCCAATGAAGAGCCCAGTGGCTGGCTACACATCTTTCTTCAGAGCCTTACCACTTCAAGTCTTAGAACAATCATTCCAAATCACAACAGCTGAGGCTCAGCAACTTAAGCAAACAAGAAGGCAACACACTTTCCTCTTCCCTCCAACCAGCGGCAGTAGCTTCAGCCGCAGCCGCAGGTCTTGA

mRNA sequence

ATGGCTGTTAAAGTTTTATTGGCGATTCTACTATGTTTCTTTGCTTGCGAGTCTCTCGTGAACGCTCGGGACCCATCTGAGAGGCGTGGGTTTCGGTTCACCACAGAAGCTCAGCTATGCAAGCTTGACAAGATTCGGGTTAGGCCACCATCACGAAGGATCGAGTCGGAGGGAGGCATCACTGAGCTTTGGGATGAGGCTGATGAAGAGTTTCAGTGTGCTGGAGTTGGTGCCATTAGAAACATCATAAGGCCCAATTCTCTCTCTTTGCCTAAATTCCACAATGCCCCTATGCTTGTTTACATCGAGCAAGGTGAAGCGTTTTTCGGGATGAACTACCCAGGGTGTGCAGAGACATACGAATCACAGTCAGCTCAATCTTCAAGGTCTACAAGGCGCATGGGACGACGAATTGGCGCCGGCAGAACAGAAGAAGACCAACACCAAAAGATTCGCAGAGTTCGACGTGGTGACATGATCGTCATTCCCGCTGGTACCGTCCAATGGTGCTACAACGATGGCGGCGAAGACCTCATTGCCGTGGCCTTCCTGGATCTCAACAATGATGACAACCAACTCGACCTCCGCGTCAGGGGATCTTTCTTGGCTGGTGGAGTTCCAAGTGAATCAAGAAGGGAAATAAGAGGATCAAAATCAGAAAATCTTGTGAACATCTTCAGTGGGTTAGATCAAGAGTTTCTTTCAGAGGCTTACAACATTCCATCAGAATTAGTGAGGAGAATGCAAGAAGAAAGGAGCAGTGGGCTCATTGTGAAGTGTGACGAAGAGATGTCCTTTTTAACTCCGGAGGAAGAAGAGGAAGAATTAAGCGAAACGTCATTTTCAAGAAGGAGAGGAGAATACTCAAATGGGATAGAAGAAACCGTATGCACTGCTAGAGTTCAACACAACATGAACACCCAAAGAGAAGCTGATCTATTCTCTAGAGAAGCTGGTAGAGTTAACATCTTGAACCAACTCAAGCTCCCTATCCTAAGATTCTTGGGCATGAGTGCTGAGAAAGGCCACCTTTTCGCGAATGCTCAACACAACTTGCATTGGTCAATGACGGATCACAGAATGGTGTACGTAGTAGACGGAGAGGCCGAAATTCAAATATCCGACGACTACGGAAACCAAGTATTCAATGAGAGAGTATCAAGAGGAAACATGTTTGTGATTCCTCAATTCTACCCTGCATTAGCTAGAGCTGGGCAAGAAGGATTTGAATGGGTAACTTTCAAGACATCAAATCAGCCAATGAAGAGCCCAGTGGCTGGCTACACATCTTTCTTCAGAGCCTTACCACTTCAAGTCTTAGAACAATCATTCCAAATCACAACAGCTGAGGCTCAGCAACTTAAGCAAACAAGAAGGCAACACACTTTCCTCTTCCCTCCAACCAGCGGCAGTAGCTTCAGCCGCAGCCGCAGGTCTTGA

Coding sequence (CDS)

ATGGCTGTTAAAGTTTTATTGGCGATTCTACTATGTTTCTTTGCTTGCGAGTCTCTCGTGAACGCTCGGGACCCATCTGAGAGGCGTGGGTTTCGGTTCACCACAGAAGCTCAGCTATGCAAGCTTGACAAGATTCGGGTTAGGCCACCATCACGAAGGATCGAGTCGGAGGGAGGCATCACTGAGCTTTGGGATGAGGCTGATGAAGAGTTTCAGTGTGCTGGAGTTGGTGCCATTAGAAACATCATAAGGCCCAATTCTCTCTCTTTGCCTAAATTCCACAATGCCCCTATGCTTGTTTACATCGAGCAAGGTGAAGCGTTTTTCGGGATGAACTACCCAGGGTGTGCAGAGACATACGAATCACAGTCAGCTCAATCTTCAAGGTCTACAAGGCGCATGGGACGACGAATTGGCGCCGGCAGAACAGAAGAAGACCAACACCAAAAGATTCGCAGAGTTCGACGTGGTGACATGATCGTCATTCCCGCTGGTACCGTCCAATGGTGCTACAACGATGGCGGCGAAGACCTCATTGCCGTGGCCTTCCTGGATCTCAACAATGATGACAACCAACTCGACCTCCGCGTCAGGGGATCTTTCTTGGCTGGTGGAGTTCCAAGTGAATCAAGAAGGGAAATAAGAGGATCAAAATCAGAAAATCTTGTGAACATCTTCAGTGGGTTAGATCAAGAGTTTCTTTCAGAGGCTTACAACATTCCATCAGAATTAGTGAGGAGAATGCAAGAAGAAAGGAGCAGTGGGCTCATTGTGAAGTGTGACGAAGAGATGTCCTTTTTAACTCCGGAGGAAGAAGAGGAAGAATTAAGCGAAACGTCATTTTCAAGAAGGAGAGGAGAATACTCAAATGGGATAGAAGAAACCGTATGCACTGCTAGAGTTCAACACAACATGAACACCCAAAGAGAAGCTGATCTATTCTCTAGAGAAGCTGGTAGAGTTAACATCTTGAACCAACTCAAGCTCCCTATCCTAAGATTCTTGGGCATGAGTGCTGAGAAAGGCCACCTTTTCGCGAATGCTCAACACAACTTGCATTGGTCAATGACGGATCACAGAATGGTGTACGTAGTAGACGGAGAGGCCGAAATTCAAATATCCGACGACTACGGAAACCAAGTATTCAATGAGAGAGTATCAAGAGGAAACATGTTTGTGATTCCTCAATTCTACCCTGCATTAGCTAGAGCTGGGCAAGAAGGATTTGAATGGGTAACTTTCAAGACATCAAATCAGCCAATGAAGAGCCCAGTGGCTGGCTACACATCTTTCTTCAGAGCCTTACCACTTCAAGTCTTAGAACAATCATTCCAAATCACAACAGCTGAGGCTCAGCAACTTAAGCAAACAAGAAGGCAACACACTTTCCTCTTCCCTCCAACCAGCGGCAGTAGCTTCAGCCGCAGCCGCAGGTCTTGA

Protein sequence

MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGITELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETYESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIAVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNIPSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTARVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHRMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQPMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS*
Homology
BLAST of CSPI03G22540 vs. ExPASy Swiss-Prot
Match: Q9XHP0 (11S globulin seed storage protein 2 OS=Sesamum indicum OX=4182 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 2.7e-120
Identity = 219/468 (46.79%), Postives = 308/468 (65.81%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           +A K LLA+ L      ++   R+P         T+ Q C+  +I    PS RI+SEGG 
Sbjct: 2   VAFKFLLALSLSLLVSAAIAQTREPR-------LTQGQQCRFQRISGAQPSLRIQSEGGT 61

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDE  E+FQCAG+ A+R+ IRPN LSLP +H +P LVYIE+G+    +  PGCAETY
Sbjct: 62  TELWDERQEQFQCAGIVAMRSTIRPNGLSLPNYHPSPRLVYIERGQGLISIMVPGCAETY 121

Query: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180
           +   +Q +        +   G +  D HQK+ R+R+GD++ IP+G   WCYNDG EDL+A
Sbjct: 122 QVHRSQRTMERTEASEQQDRG-SVRDLHQKVHRLRQGDIVAIPSGAAHWCYNDGSEDLVA 181

Query: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI 240
           V+  D+N+  NQLD + R  +LAGGVP    +E +  ++ +  NIF   D E LSEA+N+
Sbjct: 182 VSINDVNHLSNQLDQKFRAFYLAGGVPRSGEQEQQARQTFH--NIFRAFDAELLSEAFNV 241

Query: 241 PSELVRRMQ-EERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTA 300
           P E +RRMQ EE   GLIV   E M+F+ P+EEE E        R  +  NG+EET CT 
Sbjct: 242 PQETIRRMQSEEEERGLIVMARERMTFVRPDEEEGEQ-----EHRGRQLDNGLEETFCTM 301

Query: 301 RVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDH 360
           + + N+ ++READ+FSR+AGRV+++++ KLPIL+++ +SAEKG+L++NA  +  WSMT H
Sbjct: 302 KFRTNVESRREADIFSRQAGRVHVVDRNKLPILKYMDLSAEKGNLYSNALVSPDWSMTGH 361

Query: 361 RMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQ 420
            +VYV  G+A++Q+ D  G  + N+RV++G MFV+PQ+Y + ARAG  GFEWV FKT+  
Sbjct: 362 TIVYVTRGDAQVQVVDHNGQALMNDRVNQGEMFVVPQYYTSTARAGNNGFEWVAFKTTGS 421

Query: 421 PMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPP 468
           PM+SP+AGYTS  RA+PLQV+  S+QI+  +AQ LK  R   +FL  P
Sbjct: 422 PMRSPLAGYTSVIRAMPLQVITNSYQISPNQAQALKMNRGSQSFLLSP 454

BLAST of CSPI03G22540 vs. ExPASy Swiss-Prot
Match: Q8GZP6 (11S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occidentale OX=171929 PE=1 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 2.5e-94
Identity = 182/446 (40.81%), Postives = 272/446 (60.99%), Query Frame = 0

Query: 40  CKLDKIRVRPPSRRIESEGGITELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPML 99
           C++D++    P  R+E E G  E WD   E+F+CAGV  +R+ I+PN L LP++ NAP L
Sbjct: 25  CQIDRLDALEPDNRVEYEAGTVEAWDPNHEQFRCAGVALVRHTIQPNGLLLPQYSNAPQL 84

Query: 100 VYIEQGEAFFGMNYPGCAETYESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDM 159
           +Y+ QGE   G++YPGC ETY++          + GR+ G     +D+HQKIRR RRGD+
Sbjct: 85  IYVVQGEGMTGISYPGCPETYQAP---------QQGRQQGQSGRFQDRHQKIRRFRRGDI 144

Query: 160 IVIPAGTVQWCYNDGGEDLIAVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKS 219
           I IPAG   WCYN+G   ++ V  LD++N  NQLD   R   LAG  P +  ++ +  +S
Sbjct: 145 IAIPAGVAHWCYNEGNSPVVTVTLLDVSNSQNQLDRTPRKFHLAGN-PKDVFQQQQQHQS 204

Query: 220 ENLVNIFSGLDQEFLSEAYNIPSELVRRMQEERSSGLIVKC-DEEMSFLTPE-------E 279
               N+FSG D E L+EA+ +   L+++++ E + G IVK  D+E+  + P         
Sbjct: 205 RGR-NLFSGFDTELLAEAFQVDERLIKQLKSEDNRGGIVKVKDDELRVIRPSRSQSERGS 264

Query: 280 EEEELSETSFSRRRGEYSNGIEETVCTARVQHNMNTQREADLFSREAGRVNILNQLKLPI 339
           E EE SE    RR G+  NGIEET+CT R++ N+N    AD+++ E GR+  LN L LPI
Sbjct: 265 ESEEESEDE-KRRWGQRDNGIEETICTMRLKENINDPARADIYTPEVGRLTTLNSLNLPI 324

Query: 340 LRFLGMSAEKGHLFANAQHNLHWSMTDHRMVYVVDGEAEIQISDDYGNQVFNERVSRGNM 399
           L++L +S EKG L+ NA    HW++  H ++Y   G+ ++Q+ D++GN+VF+  V  G M
Sbjct: 325 LKWLQLSVEKGVLYKNALVLPHWNLNSHSIIYGCKGKGQVQVVDNFGNRVFDGEVREGQM 384

Query: 400 FVIPQFYPALARAGQEGFEWVTFKTSNQPMKSPVAGYTSFFRALPLQVLEQSFQITTAEA 459
            V+PQ +  + RA +E FEW++FKT+++ M SP+AG TS    +P +VL  +FQI+  +A
Sbjct: 385 LVVPQNFAVVKRAREERFEWISFKTNDRAMTSPLAGRTSVLGGMPEEVLANAFQISREDA 444

Query: 460 QQLKQTRRQHTFLFPPTSGSSFSRSR 478
           +++K   +Q T     TSG S    R
Sbjct: 445 RKIKFNNQQTTL----TSGESSHHMR 454

BLAST of CSPI03G22540 vs. ExPASy Swiss-Prot
Match: B5KVH4 (11S globulin seed storage protein 1 OS=Carya illinoinensis OX=32201 PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.8e-93
Identity = 199/515 (38.64%), Postives = 287/515 (55.73%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MA  +LL+I LC     +L N      + G R   +   C+L+++    P+ RIE+E G+
Sbjct: 1   MAKPILLSIYLCLIIV-ALFNG--CLAQSGGRQQHKFGQCQLNRLDALEPTNRIEAEAGV 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
            E WD   ++ QCAGV  +R  I PN L LP + NAP LVYI +G    G+ +PGC ET+
Sbjct: 61  IESWDPNHQQLQCAGVAVVRRTIEPNGLLLPHYSNAPQLVYIARGRGITGVLFPGCPETF 120

Query: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180
           E    QS +  RR          ++D+HQKIR  R GD+I  PAG   WCYNDG   ++A
Sbjct: 121 EESQRQSQQGQRR--------EFQQDRHQKIRHFREGDIIAFPAGVAHWCYNDGSSPVVA 180

Query: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSE-------------------SRREIRGSKSEN 240
           +  LD +N+ NQLD   R  +LAG    E                    RR   G +  +
Sbjct: 181 IFLLDTHNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRRGEHGEQQRD 240

Query: 241 L-VNIFSGLDQEFLSEAYNIPSELVRRMQEERS-SGLIVKCD-EEMSFLTP--------- 300
           L  N+FSG D EFL++A+N+ +E  RR+Q E    G IV+ +  ++  + P         
Sbjct: 241 LGNNVFSGFDAEFLADAFNVDTETARRLQSENDHRGSIVRVEGRQLQVIRPRWSREEQEH 300

Query: 301 ------EEEEEELSETSFSRRRGEYSNGIEETVCTARVQHNMNTQREADLFSREAGRVNI 360
                 E E E  SE   SRR G   NG+EET+CT  ++ N+     AD+++ EAGR++ 
Sbjct: 301 EERKERERERESESERRQSRRGGRDDNGLEETICTLSLRENIGDPSRADIYTEEAGRIST 360

Query: 361 LNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHRMVYVVDGEAEIQISDDYGNQVFN 420
           +N   LPILR+L +SAE+G L+++A +  HW++  H +VY + G AE+Q+ D++G  VF+
Sbjct: 361 VNSHNLPILRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTVFD 420

Query: 421 ERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQPMKSPVAGYTSFFRALPLQVLEQS 479
           + +  G +  IPQ +  + RA  EGFEWV+FKT+   M SP+AG TS  RALP +VL  +
Sbjct: 421 DELREGQLLTIPQNFAVVKRARDEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLVNA 480

BLAST of CSPI03G22540 vs. ExPASy Swiss-Prot
Match: A0A1L6K371 (11S globulin OS=Juglans nigra OX=16719 PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 6.2e-93
Identity = 199/519 (38.34%), Postives = 289/519 (55.68%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MA  +LL+I LC  A   LVN      + G R       CKL ++    PS RIE+E G+
Sbjct: 1   MAKPILLSISLCLVA---LVNG--CLAQSGGRQQPRFGECKLKRLVALEPSNRIEAEAGV 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
            E WD  +++FQCAGV  +R  I PN L LP++ NAP L+YI +G    G+ +PGC ET+
Sbjct: 61  IESWDPNNQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLLYIVKGRGITGVLFPGCPETF 120

Query: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180
           E    +S +   R+   + +   + D+HQKIR  R GD+I  PAG   WCYNDG   ++A
Sbjct: 121 E----ESQQGQSRIRPSLRSASFQRDRHQKIRHFREGDVIAFPAGVAHWCYNDGDTPVVA 180

Query: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESR------------------------REIRG 240
           VA +D  N+ NQLD   R  +LAG    E R                        ++ RG
Sbjct: 181 VALMDTTNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRHGEPGQQQRG 240

Query: 241 SKSENLVNIFSGLDQEFLSEAYNIPSELVRRMQEERS-SGLIVKCD-EEMSFLTP----- 300
           S +    N+FSG D +FL++A+N+ +E  RR+Q E      IV+ +  ++  + P     
Sbjct: 241 SGN----NVFSGFDADFLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSRE 300

Query: 301 ----------EEEEEELSETSFSRRRGEYSNGIEETVCTARVQHNMNTQREADLFSREAG 360
                     E E E  SE   SRR G   NG+EET+CT R++ N+     AD+++ EAG
Sbjct: 301 EQEREERKERERERESESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAG 360

Query: 361 RVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHRMVYVVDGEAEIQISDDYGN 420
           R++  N   LP+LR+L +SAE+G L+++A +  HW++  H +VY + G AE+Q+ D++G 
Sbjct: 361 RISTANSHTLPVLRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQ 420

Query: 421 QVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQPMKSPVAGYTSFFRALPLQV 479
            VF++ +  G +  IPQ +  + RA  EGFEWV+FKT+   M SP+AG TS  RALP +V
Sbjct: 421 TVFDDELREGQLLTIPQNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEV 480

BLAST of CSPI03G22540 vs. ExPASy Swiss-Prot
Match: Q2TPW5 (11S globulin seed storage protein Jug r 4 OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 8.1e-93
Identity = 198/515 (38.45%), Postives = 290/515 (56.31%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MA  +LL+I L  F   +L N      + G R   +   C+L+++    P+ RIE+E G+
Sbjct: 1   MAKPILLSIYL--FLIVALFNG--CLAQSGGRQQQQFGQCQLNRLDALEPTNRIEAEAGV 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
            E WD  +++FQCAGV  +R  I PN L LP++ NAP LVYI +G    G+ +PGC ET+
Sbjct: 61  IESWDPNNQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLVYIARGRGITGVLFPGCPETF 120

Query: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180
           E    QS +   R          ++D+HQKIR  R GD+I  PAG   W YNDG   ++A
Sbjct: 121 EESQRQSQQGQSR--------EFQQDRHQKIRHFREGDIIAFPAGVAHWSYNDGSNPVVA 180

Query: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESR---------------REIR----GSKSEN 240
           ++ LD NN+ NQLD   R  +LAG    E R               R+ R    G +   
Sbjct: 181 ISLLDTNNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQRQQRPGEHGQQQRG 240

Query: 241 L-VNIFSGLDQEFLSEAYNIPSELVRRMQEERS-SGLIVKCD-EEMSFLTP--------- 300
           L  N+FSG D +FL++A+N+ +E  RR+Q E      IV+ +  ++  + P         
Sbjct: 241 LGNNVFSGFDADFLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSREEQER 300

Query: 301 ------EEEEEELSETSFSRRRGEYSNGIEETVCTARVQHNMNTQREADLFSREAGRVNI 360
                 E E E  SE   SRR G   NG+EET+CT R++ N+     AD+++ EAGR++ 
Sbjct: 301 EERKERERERESESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAGRIST 360

Query: 361 LNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHRMVYVVDGEAEIQISDDYGNQVFN 420
           +N   LP+LR+L +SAE+G L+++A +  HW++  H +VY + G AE+Q+ D++G  VF+
Sbjct: 361 VNSHTLPVLRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTVFD 420

Query: 421 ERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQPMKSPVAGYTSFFRALPLQVLEQS 479
           + +  G +  IPQ +  + RA  EGFEWV+FKT+   M SP+AG TS  RALP +VL  +
Sbjct: 421 DELREGQLLTIPQNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLATA 480

BLAST of CSPI03G22540 vs. ExPASy TrEMBL
Match: A0A0A0L7Y5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G386810 PE=3 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 7.7e-272
Identity = 478/479 (99.79%), Postives = 478/479 (99.79%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI
Sbjct: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY
Sbjct: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120

Query: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180
           ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA
Sbjct: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180

Query: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI 240
           VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI
Sbjct: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI 240

Query: 241 PSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTAR 300
           PSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGE SNGIEETVCTAR
Sbjct: 241 PSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEDSNGIEETVCTAR 300

Query: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHR 360
           VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHR
Sbjct: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHR 360

Query: 361 MVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420
           MVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP
Sbjct: 361 MVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420

Query: 421 MKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS 480
           MKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS
Sbjct: 421 MKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS 479

BLAST of CSPI03G22540 vs. ExPASy TrEMBL
Match: A0A5D3DAK7 (11S globulin seed storage protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G001310 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 3.8e-263
Identity = 460/479 (96.03%), Postives = 472/479 (98.54%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MAVKVLLAILLCFFACESLVNA+DP+ERR FRFTTEAQLCKLD+IRVRPPSRRIESEGGI
Sbjct: 1   MAVKVLLAILLCFFACESLVNAQDPAERRRFRFTTEAQLCKLDRIRVRPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEADEEFQCAGVGAIRN IRPNSLSLPKFHNAPMLVYIEQGE FFGMNYPGCAETY
Sbjct: 61  TELWDEADEEFQCAGVGAIRNTIRPNSLSLPKFHNAPMLVYIEQGEGFFGMNYPGCAETY 120

Query: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180
           ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA
Sbjct: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180

Query: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI 240
           VAFLDLNNDDNQLDLRVRGSFLAGGVPSE+RREIRGSKS+NLVNIF+GLDQEFLSEA+NI
Sbjct: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSEARREIRGSKSDNLVNIFNGLDQEFLSEAFNI 240

Query: 241 PSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTAR 300
           PS+LVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETS+SRRRGE  NGIEET+CTAR
Sbjct: 241 PSDLVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSYSRRRGE-DNGIEETICTAR 300

Query: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHR 360
           VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLF NAQHNLHWSMTDHR
Sbjct: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFPNAQHNLHWSMTDHR 360

Query: 361 MVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420
           MVYVVDGEAEIQISDDYGNQ+FNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP
Sbjct: 361 MVYVVDGEAEIQISDDYGNQIFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420

Query: 421 MKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS 480
           MKSPVAGYTSFFRALPLQ+LEQSFQITTAEAQQLKQTRRQHT LFPPTSGSSFSRSRRS
Sbjct: 421 MKSPVAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTLLFPPTSGSSFSRSRRS 478

BLAST of CSPI03G22540 vs. ExPASy TrEMBL
Match: A0A1S3BHE4 (11S globulin seed storage protein 2-like OS=Cucumis melo OX=3656 GN=LOC103489874 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 3.8e-263
Identity = 460/479 (96.03%), Postives = 472/479 (98.54%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MAVKVLLAILLCFFACESLVNA+DP+ERR FRFTTEAQLCKLD+IRVRPPSRRIESEGGI
Sbjct: 1   MAVKVLLAILLCFFACESLVNAQDPAERRRFRFTTEAQLCKLDRIRVRPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEADEEFQCAGVGAIRN IRPNSLSLPKFHNAPMLVYIEQGE FFGMNYPGCAETY
Sbjct: 61  TELWDEADEEFQCAGVGAIRNTIRPNSLSLPKFHNAPMLVYIEQGEGFFGMNYPGCAETY 120

Query: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180
           ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA
Sbjct: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180

Query: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI 240
           VAFLDLNNDDNQLDLRVRGSFLAGGVPSE+RREIRGSKS+NLVNIF+GLDQEFLSEA+NI
Sbjct: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSEARREIRGSKSDNLVNIFNGLDQEFLSEAFNI 240

Query: 241 PSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTAR 300
           PS+LVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETS+SRRRGE  NGIEET+CTAR
Sbjct: 241 PSDLVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSYSRRRGE-DNGIEETICTAR 300

Query: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHR 360
           VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLF NAQHNLHWSMTDHR
Sbjct: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFPNAQHNLHWSMTDHR 360

Query: 361 MVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420
           MVYVVDGEAEIQISDDYGNQ+FNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP
Sbjct: 361 MVYVVDGEAEIQISDDYGNQIFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420

Query: 421 MKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS 480
           MKSPVAGYTSFFRALPLQ+LEQSFQITTAEAQQLKQTRRQHT LFPPTSGSSFSRSRRS
Sbjct: 421 MKSPVAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTLLFPPTSGSSFSRSRRS 478

BLAST of CSPI03G22540 vs. ExPASy TrEMBL
Match: A0A6J1HV45 (11S globulin seed storage protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111467114 PE=3 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.9e-223
Identity = 389/478 (81.38%), Postives = 437/478 (91.42%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MA +V+LAILLC  AC S   +    ER  FR   EAQ C+LD+IR  PPSRRIESEGGI
Sbjct: 1   MATRVVLAILLCLVACGS---SSQLHERHSFR--EEAQQCRLDRIRSMPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEA+E+FQCAGV A+RNIIRPN LSLPKFH++PML+YIEQGE F G+NYPGCAETY
Sbjct: 61  TELWDEAEEDFQCAGVAAVRNIIRPNCLSLPKFHSSPMLIYIEQGEGFLGLNYPGCAETY 120

Query: 121 ESQSAQSS-RSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLI 180
           E+QSAQSS RS+RRMGRRIGAG+ E+DQHQK+RRVRRGDMIV+PAGTVQWC+NDGG+DLI
Sbjct: 121 EAQSAQSSRRSSRRMGRRIGAGK-EDDQHQKVRRVRRGDMIVVPAGTVQWCHNDGGQDLI 180

Query: 181 AVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYN 240
           A+AFLDLNN+DNQLDLR+RGSFLAGG+P E RRE+RGSK+ +LVNIFSG DQEFL++AYN
Sbjct: 181 AIAFLDLNNEDNQLDLRIRGSFLAGGIPREERRELRGSKNNDLVNIFSGFDQEFLAQAYN 240

Query: 241 IPSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTA 300
           +P++LVR+MQEERSSGLIVKCDE+MSFLTPEEEEEELSE+    RR E SNG+EET+CTA
Sbjct: 241 VPTDLVRKMQEERSSGLIVKCDEQMSFLTPEEEEEELSES--PSRREEISNGLEETICTA 300

Query: 301 RVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDH 360
           RVQHNMNTQREAD++SREAGRVNILNQLKLPILRF+GMSAEKGHLF+NAQ+NLHWSMTDH
Sbjct: 301 RVQHNMNTQREADVYSREAGRVNILNQLKLPILRFMGMSAEKGHLFSNAQYNLHWSMTDH 360

Query: 361 RMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQ 420
           R+VYVVDGEA  QISDDYGNQVFNERVSRGNMFVIPQFYPAL +AGQEGFEW+TFKTSNQ
Sbjct: 361 RLVYVVDGEANFQISDDYGNQVFNERVSRGNMFVIPQFYPALVQAGQEGFEWITFKTSNQ 420

Query: 421 PMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSR 478
           PMKSP+AGYTSFFRALPLQ+LEQSFQITTAEAQQLKQTRRQHTFLFPP+SGSS  R R
Sbjct: 421 PMKSPIAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTFLFPPSSGSSSRRHR 470

BLAST of CSPI03G22540 vs. ExPASy TrEMBL
Match: A0A6J1HGI1 (11S globulin seed storage protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464084 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 4.3e-222
Identity = 387/480 (80.62%), Postives = 437/480 (91.04%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           M  +V+LAILLC  AC S   +    ERR FR   EAQ C+LD+IR  PPSRRIESEGGI
Sbjct: 1   MPTRVVLAILLCLVACGS---SSQLHERRSFR--EEAQQCRLDRIRSLPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEA+E+FQCAGV AIRNIIRPN LSLPKFH++PML+YIEQGE F G+N+PGCAETY
Sbjct: 61  TELWDEAEEDFQCAGVAAIRNIIRPNCLSLPKFHSSPMLIYIEQGEGFLGLNFPGCAETY 120

Query: 121 ESQSAQSS-RSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLI 180
           E+QSAQSS RS+RRMGRRIGAG+ E+DQHQK+RRVRRGDMIV+PAGTVQWC+NDGG+DL+
Sbjct: 121 EAQSAQSSRRSSRRMGRRIGAGK-EDDQHQKVRRVRRGDMIVVPAGTVQWCHNDGGQDLV 180

Query: 181 AVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYN 240
           AVAFLDLNN+DNQLDLR+RGSFLAGG+P E RRE+RGSK+ +LVNI++G DQ+FL++AYN
Sbjct: 181 AVAFLDLNNEDNQLDLRIRGSFLAGGIPREERRELRGSKNNDLVNIYNGFDQDFLAQAYN 240

Query: 241 IPSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTA 300
           +P++LVRRMQEERSSGLIVKCDE+MSFLTPEEEEEELSE+    RR E SNG+EET+CTA
Sbjct: 241 VPTDLVRRMQEERSSGLIVKCDEQMSFLTPEEEEEELSES--PSRREEISNGLEETICTA 300

Query: 301 RVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDH 360
           RVQHNMNTQREAD++SREAGR+NILNQLKLPILRF+GMSAEKGHLF NAQ+NLHWSMTDH
Sbjct: 301 RVQHNMNTQREADVYSREAGRINILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDH 360

Query: 361 RMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQ 420
           R+VYVVDGEA  QISDDYGNQVFNERVSRGNMFVIPQFYPAL +AGQEGFEW+TFKTSNQ
Sbjct: 361 RLVYVVDGEANFQISDDYGNQVFNERVSRGNMFVIPQFYPALVQAGQEGFEWITFKTSNQ 420

Query: 421 PMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS 480
           PMKSP+AGYTSFFRALPLQ+LEQSFQITTAEAQQLKQTRRQHTFLFPP+SG S SR  RS
Sbjct: 421 PMKSPIAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTFLFPPSSGGSSSRRHRS 472

BLAST of CSPI03G22540 vs. NCBI nr
Match: XP_011652795.1 (11S globulin seed storage protein 2 [Cucumis sativus] >KGN57908.1 hypothetical protein Csa_009501 [Cucumis sativus])

HSP 1 Score: 945.7 bits (2443), Expect = 1.6e-271
Identity = 478/479 (99.79%), Postives = 478/479 (99.79%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI
Sbjct: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY
Sbjct: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120

Query: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180
           ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA
Sbjct: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180

Query: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI 240
           VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI
Sbjct: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI 240

Query: 241 PSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTAR 300
           PSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGE SNGIEETVCTAR
Sbjct: 241 PSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEDSNGIEETVCTAR 300

Query: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHR 360
           VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHR
Sbjct: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHR 360

Query: 361 MVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420
           MVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP
Sbjct: 361 MVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420

Query: 421 MKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS 480
           MKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS
Sbjct: 421 MKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS 479

BLAST of CSPI03G22540 vs. NCBI nr
Match: XP_008447427.1 (PREDICTED: 11S globulin seed storage protein 2-like [Cucumis melo] >KAA0038059.1 11S globulin seed storage protein 2-like [Cucumis melo var. makuwa] >TYK20544.1 11S globulin seed storage protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 916.8 bits (2368), Expect = 8.0e-263
Identity = 460/479 (96.03%), Postives = 472/479 (98.54%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MAVKVLLAILLCFFACESLVNA+DP+ERR FRFTTEAQLCKLD+IRVRPPSRRIESEGGI
Sbjct: 1   MAVKVLLAILLCFFACESLVNAQDPAERRRFRFTTEAQLCKLDRIRVRPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEADEEFQCAGVGAIRN IRPNSLSLPKFHNAPMLVYIEQGE FFGMNYPGCAETY
Sbjct: 61  TELWDEADEEFQCAGVGAIRNTIRPNSLSLPKFHNAPMLVYIEQGEGFFGMNYPGCAETY 120

Query: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180
           ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA
Sbjct: 121 ESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIA 180

Query: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYNI 240
           VAFLDLNNDDNQLDLRVRGSFLAGGVPSE+RREIRGSKS+NLVNIF+GLDQEFLSEA+NI
Sbjct: 181 VAFLDLNNDDNQLDLRVRGSFLAGGVPSEARREIRGSKSDNLVNIFNGLDQEFLSEAFNI 240

Query: 241 PSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTAR 300
           PS+LVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETS+SRRRGE  NGIEET+CTAR
Sbjct: 241 PSDLVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSYSRRRGE-DNGIEETICTAR 300

Query: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHR 360
           VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLF NAQHNLHWSMTDHR
Sbjct: 301 VQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFPNAQHNLHWSMTDHR 360

Query: 361 MVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420
           MVYVVDGEAEIQISDDYGNQ+FNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP
Sbjct: 361 MVYVVDGEAEIQISDDYGNQIFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQP 420

Query: 421 MKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS 480
           MKSPVAGYTSFFRALPLQ+LEQSFQITTAEAQQLKQTRRQHT LFPPTSGSSFSRSRRS
Sbjct: 421 MKSPVAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTLLFPPTSGSSFSRSRRS 478

BLAST of CSPI03G22540 vs. NCBI nr
Match: XP_038888146.1 (11S globulin seed storage protein 2-like [Benincasa hispida])

HSP 1 Score: 849.7 bits (2194), Expect = 1.2e-242
Identity = 430/479 (89.77%), Postives = 457/479 (95.41%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MA+KV+LAILLCF ACESLVNAR+ SERR +RF+TEAQLCKLD+IRVRPPSRRIESEGGI
Sbjct: 1   MAMKVVLAILLCFVACESLVNARE-SERRQYRFSTEAQLCKLDRIRVRPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEADEEFQCAGVGAIRN+IRPNSLSLPKFHNAPMLVYIEQGE F GMNYPGCAETY
Sbjct: 61  TELWDEADEEFQCAGVGAIRNLIRPNSLSLPKFHNAPMLVYIEQGEGFLGMNYPGCAETY 120

Query: 121 ESQSAQSS-RSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLI 180
           ESQS QSS RSTRRMGRRIGAGR+ EDQHQKIRR+RRGD+IVIPAGTVQWCYNDG +DLI
Sbjct: 121 ESQSPQSSRRSTRRMGRRIGAGRSGEDQHQKIRRIRRGDIIVIPAGTVQWCYNDGPQDLI 180

Query: 181 AVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYN 240
           AVAFLDLNNDDNQLDLR+RGSFLAGGVPSE+RREIRGSKS++LVNIF+G DQE L+EAYN
Sbjct: 181 AVAFLDLNNDDNQLDLRIRGSFLAGGVPSEARREIRGSKSDDLVNIFNGFDQELLAEAYN 240

Query: 241 IPSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTA 300
           IP+EL RRMQEERSSGLIVKCDEEMSFLTPE EEEELSE+ FSRR  E  NGIEET+CTA
Sbjct: 241 IPTELARRMQEERSSGLIVKCDEEMSFLTPELEEEELSESPFSRRGEE--NGIEETICTA 300

Query: 301 RVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDH 360
           RV HNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLF+NAQHNLHWSMTDH
Sbjct: 301 RVVHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFSNAQHNLHWSMTDH 360

Query: 361 RMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQ 420
           R+VYV+DGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQ
Sbjct: 361 RLVYVIDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQ 420

Query: 421 PMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRR 479
           PMKSPV GYTSFFRALPLQ+LEQ+FQITTAEAQQLKQTRRQHTFLFPP+S S+F RSRR
Sbjct: 421 PMKSPVVGYTSFFRALPLQLLEQAFQITTAEAQQLKQTRRQHTFLFPPSSSSTF-RSRR 475

BLAST of CSPI03G22540 vs. NCBI nr
Match: XP_022967670.1 (11S globulin seed storage protein 2-like [Cucurbita maxima])

HSP 1 Score: 783.9 bits (2023), Expect = 8.0e-223
Identity = 389/478 (81.38%), Postives = 437/478 (91.42%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MA +V+LAILLC  AC S   +    ER  FR   EAQ C+LD+IR  PPSRRIESEGGI
Sbjct: 1   MATRVVLAILLCLVACGS---SSQLHERHSFR--EEAQQCRLDRIRSMPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEA+E+FQCAGV A+RNIIRPN LSLPKFH++PML+YIEQGE F G+NYPGCAETY
Sbjct: 61  TELWDEAEEDFQCAGVAAVRNIIRPNCLSLPKFHSSPMLIYIEQGEGFLGLNYPGCAETY 120

Query: 121 ESQSAQSS-RSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLI 180
           E+QSAQSS RS+RRMGRRIGAG+ E+DQHQK+RRVRRGDMIV+PAGTVQWC+NDGG+DLI
Sbjct: 121 EAQSAQSSRRSSRRMGRRIGAGK-EDDQHQKVRRVRRGDMIVVPAGTVQWCHNDGGQDLI 180

Query: 181 AVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYN 240
           A+AFLDLNN+DNQLDLR+RGSFLAGG+P E RRE+RGSK+ +LVNIFSG DQEFL++AYN
Sbjct: 181 AIAFLDLNNEDNQLDLRIRGSFLAGGIPREERRELRGSKNNDLVNIFSGFDQEFLAQAYN 240

Query: 241 IPSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTA 300
           +P++LVR+MQEERSSGLIVKCDE+MSFLTPEEEEEELSE+    RR E SNG+EET+CTA
Sbjct: 241 VPTDLVRKMQEERSSGLIVKCDEQMSFLTPEEEEEELSES--PSRREEISNGLEETICTA 300

Query: 301 RVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDH 360
           RVQHNMNTQREAD++SREAGRVNILNQLKLPILRF+GMSAEKGHLF+NAQ+NLHWSMTDH
Sbjct: 301 RVQHNMNTQREADVYSREAGRVNILNQLKLPILRFMGMSAEKGHLFSNAQYNLHWSMTDH 360

Query: 361 RMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQ 420
           R+VYVVDGEA  QISDDYGNQVFNERVSRGNMFVIPQFYPAL +AGQEGFEW+TFKTSNQ
Sbjct: 361 RLVYVVDGEANFQISDDYGNQVFNERVSRGNMFVIPQFYPALVQAGQEGFEWITFKTSNQ 420

Query: 421 PMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSR 478
           PMKSP+AGYTSFFRALPLQ+LEQSFQITTAEAQQLKQTRRQHTFLFPP+SGSS  R R
Sbjct: 421 PMKSPIAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTFLFPPSSGSSSRRHR 470

BLAST of CSPI03G22540 vs. NCBI nr
Match: KAG6571523.1 (hypothetical protein SDJN03_28251, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 783.9 bits (2023), Expect = 8.0e-223
Identity = 389/480 (81.04%), Postives = 439/480 (91.46%), Query Frame = 0

Query: 1   MAVKVLLAILLCFFACESLVNARDPSERRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGI 60
           MA +V+LAILLC  AC S   +    ERR FR   EAQ C+LD+IR  PPSRRIESEGGI
Sbjct: 1   MATRVVLAILLCLVACGS---SSQLYERRSFR--EEAQQCRLDRIRSMPPSRRIESEGGI 60

Query: 61  TELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETY 120
           TELWDEA+E+FQCAGV AIRNIIRPN LSLPKFH++PML+YIEQGE F G+N+PGCAETY
Sbjct: 61  TELWDEAEEDFQCAGVAAIRNIIRPNCLSLPKFHSSPMLIYIEQGEGFLGLNFPGCAETY 120

Query: 121 ESQSAQSS-RSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLI 180
           E+QSAQSS RS+RRMGRRIGAG+ E+DQHQK+RRVRRGDMIV+PAGTVQWC+NDGG+DL+
Sbjct: 121 EAQSAQSSRRSSRRMGRRIGAGK-EDDQHQKVRRVRRGDMIVVPAGTVQWCHNDGGQDLV 180

Query: 181 AVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGLDQEFLSEAYN 240
           AVAFLDLNN+DNQLDLR+RGSFLAGG+P E RRE+RGSK+ +LVNI++G DQ+FL++AYN
Sbjct: 181 AVAFLDLNNEDNQLDLRIRGSFLAGGIPREERRELRGSKNNDLVNIYNGFDQDFLAQAYN 240

Query: 241 IPSELVRRMQEERSSGLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYSNGIEETVCTA 300
           +P++LVRRMQEERSSGLIVKCDE+MSFLTPEEEEEELSE+    RR E SNG+EET+CTA
Sbjct: 241 VPTDLVRRMQEERSSGLIVKCDEQMSFLTPEEEEEELSES--PSRREEISNGLEETICTA 300

Query: 301 RVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDH 360
           RVQHNMNTQREAD++SREAGR+NILNQLKLPILRF+GMSAEKGHLF NAQ+NLHWSMTDH
Sbjct: 301 RVQHNMNTQREADVYSREAGRINILNQLKLPILRFMGMSAEKGHLFPNAQYNLHWSMTDH 360

Query: 361 RMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQ 420
           R+VYVVDGEA  QISDDYGNQVFNERVSRGNMFVIPQFYPAL +AGQEGFEW+TFKTSNQ
Sbjct: 361 RLVYVVDGEANFQISDDYGNQVFNERVSRGNMFVIPQFYPALVQAGQEGFEWITFKTSNQ 420

Query: 421 PMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSRRS 480
           PMKSP+AGYTSFFRALPLQ+LEQSFQITTAEAQQLKQTRRQHTFLFPP+SGSS SR  RS
Sbjct: 421 PMKSPIAGYTSFFRALPLQLLEQSFQITTAEAQQLKQTRRQHTFLFPPSSGSSSSRRHRS 472

BLAST of CSPI03G22540 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-74
Identity = 151/433 (34.87%), Postives = 235/433 (54.27%), Query Frame = 0

Query: 40  CKLDKIRVRPPSRRIESEGGITELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPML 99
           C   +I    P++  + E G  E+WD    E +CAGV   R  ++PNS+ LP F + P L
Sbjct: 36  CHFSQINSLAPAQATKFEAGQMEVWDHMSPELRCAGVTVARITLQPNSIFLPAFFSPPAL 95

Query: 100 VYIEQGEAFFGMNYPGCAETYESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDM 159
            Y+ QGE   G    GC ET+      S R     G     GR  ED HQK+   RRGD+
Sbjct: 96  AYVVQGEGVMGTIASGCPETFAEVEGSSGR-----GGGGDPGRRFEDMHQKLENFRRGDV 155

Query: 160 IVIPAGTVQWCYNDGGEDLIAVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKS 219
               AG  QW YN G  D + V  LD+ N +NQLD   R   LAG    E  + +     
Sbjct: 156 FASLAGVSQWWYNRGDSDAVIVIVLDVTNRENQLDQVPRMFQLAGSRTQEEEQPLTWPSG 215

Query: 220 ENLVNIFSGLDQEFLSEAYNIPSELVRRMQEERSS-GLIVKCDEEMSFLTPEEEEEELSE 279
               N FSG D   ++EA+ I  E  +++Q ++ + G I++ +  + F+ P   E +   
Sbjct: 216 N---NAFSGFDPNIIAEAFKINIETAKQLQNQKDNRGNIIRANGPLHFVIPPPREWQ--- 275

Query: 280 TSFSRRRGEYSNGIEETVCTARVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMS 339
                 +   +NGIEET CTA++  N++    +D FS  AGR++ LN L LP+LR + ++
Sbjct: 276 ------QDGIANGIEETYCTAKIHENIDDPERSDHFSTRAGRISTLNSLNLPVLRLVRLN 335

Query: 340 AEKGHLFANAQHNLHWSMTDHRMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFY 399
           A +G+L++       W+   H ++YV  G+A+IQ+ DD G  VFNE+V +G + VIPQ +
Sbjct: 336 ALRGYLYSGGMVLPQWTANAHTVLYVTGGQAKIQVVDDNGQSVFNEQVGQGQIIVIPQGF 395

Query: 400 PALARAGQEGFEWVTFKTSNQPMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTR 459
                AG+ GFEW++FKT++    + ++G TS+ RA+P+ V++ S+ +   EA+++K ++
Sbjct: 396 AVSKTAGETGFEWISFKTNDNAYINTLSGQTSYLRAVPVDVIKASYGVNEEEAKRIKFSQ 451

Query: 460 RQHTFLFPPTSGS 472
           ++      P+S S
Sbjct: 456 QETMLSMTPSSSS 451

BLAST of CSPI03G22540 vs. TAIR 10
Match: AT5G44120.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-72
Identity = 160/459 (34.86%), Postives = 247/459 (53.81%), Query Frame = 0

Query: 28  RRGFRFTTEAQLCKLDKIRVRPPSRRIESEGGITELWDEADEEFQCAGVGAIRNIIRPNS 87
           ++G +F  E   C+LD++    PS  ++SE G  E+WD    + +C+GV   R II    
Sbjct: 27  QQGQQFPNE---CQLDQLNALEPSHVLKSEAGRIEVWDHHAPQLRCSGVSFARYIIESKG 86

Query: 88  LSLPKFHNAPMLVYIEQGEAFFGMNYPGCAETYESQSAQSSRSTRRMGRRIGAGRTEEDQ 147
           L LP F N   L ++ +G    G   PGCAET++  S    R   +     G  +   D 
Sbjct: 87  LYLPSFFNTAKLSFVAKGRGLMGKVIPGCAETFQDSSEFQPRFEGQ-----GQSQRFRDM 146

Query: 148 HQKIRRVRRGDMIVIPAGTVQWCYNDGGEDLIAVAFLDLNNDDNQLDLRVRGSFLAGGVP 207
           HQK+  +R GD I    G  QW YNDG E L+ V+  DL +  NQLD   R  +LAG  P
Sbjct: 147 HQKVEHIRSGDTIATTPGVAQWFYNDGQEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNP 206

Query: 208 SESRREIRGSKSENLVNIFSGLDQEFLSEAYNIPSELVRRMQ-EERSSGLIVKCDEEMSF 267
            + +  ++G + +   NIF+G   E +++A  I  +  +++Q ++ + G IV+       
Sbjct: 207 -QGQVWLQGREQQPQKNIFNGFGPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGV 266

Query: 268 LTP--------EEEEEELSETSFSRRRGEYSNGIEETVCTARVQHNMNTQREADLFSREA 327
           + P        EEEEEE        R G + NG+EET+C+AR   N++    AD++  + 
Sbjct: 267 IRPPLRGQRPQEEEEEE-------GRHGRHGNGLEETICSARCTDNLDDPSRADVYKPQL 326

Query: 328 GRVNILNQLKLPILRFLGMSAEKGHLFANAQHNLHWSMTDHRMVYVVDGEAEIQISDDYG 387
           G ++ LN   LPILRF+ +SA +G +  NA     W+   + ++YV DGEA+IQI +D G
Sbjct: 327 GYISTLNSYDLPILRFIRLSALRGSIRQNAMVLPQWNANANAILYVTDGEAQIQIVNDNG 386

Query: 388 NQVFNERVSRGNMFVIPQFYPALARAGQEGFEWVTFKTSNQPMKSPVAGYTSFFRALPLQ 447
           N+VF+ +VS+G +  +PQ +  + RA    F+WV FKT+     + +AG TS  R LPL+
Sbjct: 387 NRVFDGQVSQGQLIAVPQGFSVVKRATSNRFQWVEFKTNANAQINTLAGRTSVLRGLPLE 446

Query: 448 VLEQSFQITTAEAQQLKQTRRQHTFLFPPTSGSSFSRSR 478
           V+   FQI+  EA+++K    + T L   +  +S+ R R
Sbjct: 447 VITNGFQISPEEARRVKFNTLE-TTLTHSSGPASYGRPR 468

BLAST of CSPI03G22540 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 267.3 bits (682), Expect = 2.4e-71
Identity = 153/441 (34.69%), Postives = 240/441 (54.42%), Query Frame = 0

Query: 40  CKLDKIRVRPPSRRIESEGGITELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPML 99
           C+LD++    PS+ I+SEGG  E+WD    + +C+G    R +I P  L LP F NA  L
Sbjct: 30  CQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKL 89

Query: 100 VYIEQGEAFFGMNYPGCAETYESQSAQSSRSTRRMGRRIGAGRTE--EDQHQKIRRVRRG 159
            ++  G    G   PGCAET+              G   G G+++   D HQK+  +R G
Sbjct: 90  TFVVHGRGLMGRVIPGCAETFMESPV--------FGEGQGQGQSQGFRDMHQKVEHLRCG 149

Query: 160 DMIVIPAGTVQWCYNDGGEDLIAVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGS 219
           D I  P+G  QW YN+G E LI VA  DL ++ NQLD  +R   +AG  P + +  ++G 
Sbjct: 150 DTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNP-QGQEWLQGR 209

Query: 220 KSENLVNIFSGLDQEFLSEAYNIPSELVRRMQEERSS-GLIVKCDEEMSFLTPEEEEEEL 279
           K +   NIF+G   E L++A+ I  E  +++Q ++ + G IVK +     + P      L
Sbjct: 210 KQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRP-----PL 269

Query: 280 SETSFSRRRGEYSNGIEETVCTARVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLG 339
                 ++  E +NG+EET+CT R   N++   +AD++    G ++ LN   LPILR L 
Sbjct: 270 RRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRLLR 329

Query: 340 MSAEKGHLFANAQHNLHWSMTDHRMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQ 399
           +SA +G +  NA     W++  +  +YV +G+A IQ+ +D G +VF++ +S G + V+PQ
Sbjct: 330 LSALRGSIRKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQ 389

Query: 400 FYPALARAGQEGFEWVTFKTSNQPMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQ 459
            +  +  A  E FEW+ FKT+     + +AG TS  R LPL+V+   +QI+  EA+++K 
Sbjct: 390 GFSVMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVITNGYQISPEEAKRVKF 449

Query: 460 TRRQHTFLFPPTSGSSFSRSR 478
           +  + T     +S  S+ R R
Sbjct: 450 STIETTLTH--SSPMSYGRPR 454

BLAST of CSPI03G22540 vs. TAIR 10
Match: AT4G28520.1 (cruciferin 3 )

HSP 1 Score: 230.7 bits (587), Expect = 2.4e-60
Identity = 145/466 (31.12%), Postives = 226/466 (48.50%), Query Frame = 0

Query: 40  CKLDKIRVRPPSRRIESEGGITELWDEADEEFQCAGVGAIRNIIRPNSLSLPKFHNAPML 99
           C LD + V   +  I+SE G  E WD    + +C GV   R +I    L LP F  +P +
Sbjct: 37  CNLDNLDVLQATETIKSEAGQIEYWDHNHPQLRCVGVSVARYVIEQGGLYLPTFFTSPKI 96

Query: 100 VYIEQGEAFFGMNYPGCAETY----------------------------ESQSAQSSRST 159
            Y+ QG    G   PGCAET+                            + Q  Q  R  
Sbjct: 97  SYVVQGTGISGRVVPGCAETFMDSQPMQGQQQGQPWQGRQGQQGQPWEGQGQQGQQGRQG 156

Query: 160 R---------RMGRRIGAGRTEE-----------DQHQKIRRVRRGDMIVIPAGTVQWCY 219
           +         + GR+   G+  E           D HQK+  VRRGD+     G+  W Y
Sbjct: 157 QPWEGQGQQGQQGRQGQQGQPWEGQGQQGQQGFRDMHQKVEHVRRGDVFANTPGSAHWIY 216

Query: 220 NDGGEDLIAVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGS-KSENLVNIFSGLD 279
           N G + L+ +A LD+ N  NQLD   R   LAG   +  +    GS + +   N++SG D
Sbjct: 217 NSGEQPLVIIALLDIANYQNQLDRNPRVFHLAG---NNQQGGFGGSQQQQEQKNLWSGFD 276

Query: 280 QEFLSEAYNIPSELVRRMQEERSS-GLIVKCDEEMSFLTPEEEEEELSETSFSRRRGEYS 339
            + +++A  I  +L +++Q ++ S G IV+       + P   +   SE  +   R    
Sbjct: 277 AQVIAQALKIDVQLAQQLQNQQDSRGNIVRVKGPFQVVRPPLRQPYESE-EWRHPRSPQG 336

Query: 340 NGIEETVCTARVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAEKGHLFANAQ 399
           NG+EET+C+ R   N++    AD++    GRV  +N   LPIL ++ +SA +G L  NA 
Sbjct: 337 NGLEETICSMRSHENIDDPARADVYKPSLGRVTSVNSYTLPILEYVRLSATRGVLQGNAM 396

Query: 400 HNLHWSMTDHRMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPALARAGQEGF 456
               ++M  + ++Y   G+  IQ+ +D G  V +++V +G + VIPQ +  + ++    F
Sbjct: 397 VLPKYNMNANEILYCTGGQGRIQVVNDNGQNVLDQQVQKGQLVVIPQGFAYVVQSHGNKF 456

BLAST of CSPI03G22540 vs. TAIR 10
Match: AT5G44120.2 (RmlC-like cupins superfamily protein )

HSP 1 Score: 219.2 bits (557), Expect = 7.4e-57
Identity = 133/377 (35.28%), Postives = 205/377 (54.38%), Query Frame = 0

Query: 110 GMNYPGCAETYESQSAQSSRSTRRMGRRIGAGRTEEDQHQKIRRVRRGDMIVIPAGTVQW 169
           G   PGCAET++  S    R   +     G  +   D HQK+  +R GD I    G  QW
Sbjct: 2   GKVIPGCAETFQDSSEFQPRFEGQ-----GQSQRFRDMHQKVEHIRSGDTIATTPGVAQW 61

Query: 170 CYNDGGEDLIAVAFLDLNNDDNQLDLRVRGSFLAGGVPSESRREIRGSKSENLVNIFSGL 229
            YNDG E L+ V+  DL +  NQLD   R  +LAG  P + +  ++G + +   NIF+G 
Sbjct: 62  FYNDGQEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNP-QGQVWLQGREQQPQKNIFNGF 121

Query: 230 DQEFLSEAYNIPSELVRRMQ-EERSSGLIVKCDEEMSFLTP--------EEEEEELSETS 289
             E +++A  I  +  +++Q ++ + G IV+       + P        EEEEEE     
Sbjct: 122 GPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEE----- 181

Query: 290 FSRRRGEYSNGIEETVCTARVQHNMNTQREADLFSREAGRVNILNQLKLPILRFLGMSAE 349
              R G + NG+EET+C+AR   N++    AD++  + G ++ LN   LPILRF+ +SA 
Sbjct: 182 --GRHGRHGNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSAL 241

Query: 350 KGHLFANAQHNLHWSMTDHRMVYVVDGEAEIQISDDYGNQVFNERVSRGNMFVIPQFYPA 409
           +G +  NA     W+   + ++YV DGEA+IQI +D GN+VF+ +VS+G +  +PQ +  
Sbjct: 242 RGSIRQNAMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSV 301

Query: 410 LARAGQEGFEWVTFKTSNQPMKSPVAGYTSFFRALPLQVLEQSFQITTAEAQQLKQTRRQ 469
           + RA    F+WV FKT+     + +AG TS  R LPL+V+   FQI+  EA+++K    +
Sbjct: 302 VKRATSNRFQWVEFKTNANAQINTLAGRTSVLRGLPLEVITNGFQISPEEARRVKFNTLE 361

Query: 470 HTFLFPPTSGSSFSRSR 478
            T L   +  +S+ R R
Sbjct: 362 -TTLTHSSGPASYGRPR 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XHP02.7e-12046.7911S globulin seed storage protein 2 OS=Sesamum indicum OX=4182 PE=1 SV=1[more]
Q8GZP62.5e-9440.8111S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occident... [more]
B5KVH42.8e-9338.6411S globulin seed storage protein 1 OS=Carya illinoinensis OX=32201 PE=1 SV=1[more]
A0A1L6K3716.2e-9338.3411S globulin OS=Juglans nigra OX=16719 PE=1 SV=1[more]
Q2TPW58.1e-9338.4511S globulin seed storage protein Jug r 4 OS=Juglans regia OX=51240 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L7Y57.7e-27299.79Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G386810 PE=3 SV=1[more]
A0A5D3DAK73.8e-26396.0311S globulin seed storage protein 2-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3BHE43.8e-26396.0311S globulin seed storage protein 2-like OS=Cucumis melo OX=3656 GN=LOC103489874... [more]
A0A6J1HV453.9e-22381.3811S globulin seed storage protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11146... [more]
A0A6J1HGI14.3e-22280.6311S globulin seed storage protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_011652795.11.6e-27199.7911S globulin seed storage protein 2 [Cucumis sativus] >KGN57908.1 hypothetical p... [more]
XP_008447427.18.0e-26396.03PREDICTED: 11S globulin seed storage protein 2-like [Cucumis melo] >KAA0038059.1... [more]
XP_038888146.11.2e-24289.7711S globulin seed storage protein 2-like [Benincasa hispida][more]
XP_022967670.18.0e-22381.3811S globulin seed storage protein 2-like [Cucurbita maxima][more]
KAG6571523.18.0e-22381.04hypothetical protein SDJN03_28251, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT1G03890.11.7e-7434.87RmlC-like cupins superfamily protein [more]
AT5G44120.31.6e-7234.86RmlC-like cupins superfamily protein [more]
AT1G03880.12.4e-7134.69cruciferin 2 [more]
AT4G28520.12.4e-6031.12cruciferin 3 [more]
AT5G44120.27.4e-5735.28RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 437..457
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 457..479
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..147
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 7..468
NoneNo IPR availablePANTHERPTHR31189:SF5411S GLOBULIN SEED STORAGE PROTEIN 2-LIKEcoord: 7..468
NoneNo IPR availableCDDcd02243cupin_11S_legumin_Ccoord: 312..466
e-value: 4.57217E-75
score: 230.824
NoneNo IPR availableCDDcd02242cupin_11S_legumin_Ncoord: 42..262
e-value: 1.81877E-99
score: 295.648
IPR00604411-S seed storage protein, plantPRINTSPR0043911SGLOBULINcoord: 407..425
score: 39.7
coord: 371..387
score: 32.97
coord: 302..319
score: 30.01
coord: 389..404
score: 34.04
coord: 325..345
score: 48.05
coord: 429..446
score: 42.36
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 45..246
e-value: 1.7E-31
score: 120.7
coord: 303..452
e-value: 2.0E-46
score: 170.2
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 306..450
e-value: 4.3E-26
score: 91.3
coord: 49..192
e-value: 2.3E-26
score: 92.2
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 306..473
e-value: 1.5E-55
score: 189.0
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 25..305
e-value: 7.1E-97
score: 325.8
IPR02237911-S seed storage protein, conserved sitePROSITEPS0030511S_SEED_STORAGEcoord: 290..312
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 38..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G22540.1CSPI03G22540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0045735 nutrient reservoir activity