CSPI03G22280 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G22280
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	ATP-dependent DNA helicase
Location	Chr3: 18725924 .. 18728851 (-)
RNA-Seq Expression	CSPI03G22280
Synteny	CSPI03G22280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACATAAAGCTTGAAACAACGAGACTTGATTTCTATAAAACACAACAATCACAAATTCGTTCGGAGTTGTATCAAGGGATCGTTGATGTTGTAAATGCAGGAGAAACGAGGGGAGACAAGGTTGGAAAACGTGTCGTTCTACCTTCAACTTTTATTGGTGGTCCTAAAAACATGCGACATCGATATTTAGATGCCATGGCCTTAGTACAACATTATGGGAAGCCAGATCTTTTCATAACAATGACTTGCAATTCTGAATGGAAGGAAATAAGAGAAGAACTTATGGAAGGACAACAATCTCACGATAGACCAGATCTAACTTCAAGAGTATCTAGAGGAAAGTTAATGGACTTAAAAGACCAAATTGTCCACAAGGAAATATTTGGAAATGTGGACAATTTGGAAAAGTTGCAGCATTCGTATATGTGATTGAATTTCAAAAACGAGGTCTTCCACACCTACACATGTTGGTAATATTGAAAAGGGATTTCAAGATCACTAATGCTGACCAATATGATAAATATGTTCGTGCAAAAATTCCAAATATTCAAATAAATCTTGCACTTCACAATGTTGTTGTGAAGCATATGTTGCATGGCCCATGTGGATAATATAACAAAAAGTGTCCGTGCATGATCAATGGAAAATGTAGATTTCATTATCCTCGACAGTTTTCCTCGAAAATAATGCAAGGAAATGATGCGTATCCAATATATAGAAGGAGAGATGACAGAACACGGGTATTTAAAATAATATTACATTTTATTATTTTATATTTATTTCTCTATAATGTCTTTCATAATAGCGTTAATTTCTTCAATTTATTTGTTAAAAAATATATCTTTACAATTATATCAGGTATCAATAAGAAAAAAGACATTTGATAATCGATGGGTTGTGCCTTATAATCCATACCTACTTTTGAGATACAATTGTCACATTAATGTTGAAATATGCTCGGGATTGAAAGCAGTTAAATATTTATACAAGCATATTTACAAGGGGCACGATAGAGCAGCTGTTTATATATCACATGATGGTGACAATAAAGGTATTGATGAAATAAGAGATTATCAAGATGCAAGATGCGTATCAGCTCAAGAGGCATTATGGAGAATTTATGATTTTAAACTGAATGAGATATATCGTTCGGTTATAAACTTACAACTTCACCTTCCAAACATGCATTGTGTTTCTTATTGGAAAAATCAAAATTTGAAGAAACTTATTCGTTGGGATCATGTCTCAAAAACAATGTTAACATAGTACTTTCATATGTGTCAAACATCTGAAATAGCCCGAAAATTTTTATATAAAGAGTTTCCTGAACATTATGTATGGGACAAACAACTACGAATTTGGAAGGAGAGGAAAAAAAGGAATAGCTATATCACGCATTGTAGAAGCAAATCCAAGGGAGGAAGAAAGATATTACTTGCGTTTGCTATTAAATCATGTCAGAGGATCGACGTCATTTGAGTCCTTATTGATAGTCAATGGAAAAAGAGTTCAAACATTTAAAGATTCAGCAAAAGAAAGAGGACTACTAGAATATGATGAAAGTGTAACAAAATGTTTGGATGAAGCAACTTCGTTTGAGATGCCTTATGCTTTTTGAAGACTTTTTGCAACAATCTTAGTGTATTGTGAATCTGTAGATGTTAGAAAACTGTGGAAAAGATATTCTCATGAATTGTTACATGATTATAGAAAATACGAGCCAGAGAATGTAGAATATCAATTTCAACACACTTTAAGAGATATAAAAGTGTTCTTAGAGTCAATAGGCAAACACATCACTATGTATGATTTGCCTGAAATCAAACATATGCCAGAAAGTGGAAACAATTGTTATATAAAATAAATAATTGATGAAAAATCAATTGTAGTATCAGATGAAGATATTCAAGCTCCTTTAAAGTTGAATGAAGACCAAAAGATTGCATATGATATTATTCTTGACATAATTGTGAGGAATAAAAGTGGTGTATTTTTTATTGATGGGCCAGGAGGAACCGGGAAAACATTTTTGTATCGTGCTTTGTTAGCTACTATAAGATCAAGAAATATGATTGCAATTGCAACAACAACATCAGCGTTGCTGCTTCAATTATGCCAGGGGGCCGAACAACACATTCTAGATTTAATATACCATTAGAAACACATGAGTCATCTATATGCAATATTTCTAAGCAAAGTAACAAGACAGAATTGATCAAGCAAGCAAAAGTAATAATTTGGGACGAGGTGCCAATGGCAAAAAAGGCCAGAATCAAAGCAGTTGACAGAACTTTTAGAGATATAATGGATAATCCATTACCATTCGGTGGAAAAATGGTTGTGTTAAGAGGAGATTTTCGACAAGTGTTACCTGTTGTCCCACGAGCTACAAGACAACAAACTATTAATGAAAGTTTGGTAAAGTCATACCTTTGGAATAAAATGGAGAAAATCCAACTAACTAAAAATATGAGAGCTCAAAGTGATACCCGTTTTGCAGAATATTTGTTGAAGATTGGAAACAGAACTGAACTGTCAACAGAAGATGATTATATTTGTCTCCCTGCCAATATCATTGTTACAGGTGCAAATGAAGAAGATTCAATATTAAAGTTATTGAATATTGTTTATCCTGATCTCAAAACGCATACTACTTCAGCAGAATACATGACTAATAGAGCAATTCTATCGACAACAAATGAGTATGTTGACCAAATCAATGAAAGAATGATTGAGATGTTTCCTGGAAAAATGGAAGAATTCATGAGTTTTGATGAAGCAATTGATGATACACATAATTATTATCATGAGGAATTTCTCAACTCATTGTTACCTAATGGTGTTCCTCCACACAAATTGTTTCTTAAAAAAGATTGTCCAGTTATACTATTGAGAAACTTAGACCCCAGGCAATGGTCTATGTAA

mRNA sequence

ATGTACATAAAGCTTGAAACAACGAGACTTGATTTCTATAAAACACAACAATCACAAATTCGTTCGGAGTTGTATCAAGGGATCGTTGATGTTGTAAATGCAGGAGAAACGAGGGGAGACAAGGTTGGAAAACGTGTCGTTCTACCTTCAACTTTTATTGGTGGTCCTAAAAACATGCGACATCGATATTTAGATGCCATGGCCTTAGTACAACATTATGGGAAGCCAGATCTTTTCATAACAATGACTTGCAATTCTGAATGGAAGGAAATAAGAGAAGAACTTATGGAAGGACAACAATCTCACGATAGACCAGATCTAACTTCAAGAGTATCTAGAGGAAAGTTAATGGACTTAAAAGACCAAATTGTCCACAAGGAAATATTTGGAAATGTGGACAATTTGGAAAAGTTGCAGCATTCGAGGAACCGGGAAAACATTTTTGTATCGTGCTTTGTTAGCTACTATAAGATCAAGAAATATGATTGCAATTGCAACAACAACATCAGCGTTGCTGCTTCAATTATGCCAGGGGGCCGAACAACACATTCTAGATTTAATATACCATTAGAAACACATGAGTCATCTATATGCAATATTTCTAAGCAAAGTAACAAGACAGAATTGATCAAGCAAGCAAAAGTAATAATTTGGGACGAGGTGCCAATGGCAAAAAAGGCCAGAATCAAAGCAGTTGACAGAACTTTTAGAGATATAATGGATAATCCATTACCATTCGGTGGAAAAATGGTTGTGTTAAGAGGAGATTTTCGACAAGTGTTACCTGTTGTCCCACGAGCTACAAGACAACAAACTATTAATGAAAGTTTGGTAAAGTCATACCTTTGGAATAAAATGGAGAAAATCCAACTAACTAAAAATATGAGAGCTCAAAGTGATACCCGTTTTGCAGAATATTTGTTGAAGATTGGAAACAGAACTGAACTGTCAACAGAAGATGATTATATTTGTCTCCCTGCCAATATCATTGTTACAGGTGCAAATGAAGAAGATTCAATATTAAAGTTATTGAATATTGTTTATCCTGATCTCAAAACGCATACTACTTCAGCAGAATACATGACTAATAGAGCAATTCTATCGACAACAAATGAGTATGTTGACCAAATCAATGAAAGAATGATTGAGATGTTTCCTGGAAAAATGGAAGAATTCATGAGTTTTGATGAAGCAATTGATGATACACATAATTATTATCATGAGGAATTTCTCAACTCATTGTTACCTAATGGTGTTCCTCCACACAAATTGTTTCTTAAAAAAGATTGTCCAGTTATACTATTGAGAAACTTAGACCCCAGGCAATGGTCTATGTAA

Coding sequence (CDS)

ATGTACATAAAGCTTGAAACAACGAGACTTGATTTCTATAAAACACAACAATCACAAATTCGTTCGGAGTTGTATCAAGGGATCGTTGATGTTGTAAATGCAGGAGAAACGAGGGGAGACAAGGTTGGAAAACGTGTCGTTCTACCTTCAACTTTTATTGGTGGTCCTAAAAACATGCGACATCGATATTTAGATGCCATGGCCTTAGTACAACATTATGGGAAGCCAGATCTTTTCATAACAATGACTTGCAATTCTGAATGGAAGGAAATAAGAGAAGAACTTATGGAAGGACAACAATCTCACGATAGACCAGATCTAACTTCAAGAGTATCTAGAGGAAAGTTAATGGACTTAAAAGACCAAATTGTCCACAAGGAAATATTTGGAAATGTGGACAATTTGGAAAAGTTGCAGCATTCGAGGAACCGGGAAAACATTTTTGTATCGTGCTTTGTTAGCTACTATAAGATCAAGAAATATGATTGCAATTGCAACAACAACATCAGCGTTGCTGCTTCAATTATGCCAGGGGGCCGAACAACACATTCTAGATTTAATATACCATTAGAAACACATGAGTCATCTATATGCAATATTTCTAAGCAAAGTAACAAGACAGAATTGATCAAGCAAGCAAAAGTAATAATTTGGGACGAGGTGCCAATGGCAAAAAAGGCCAGAATCAAAGCAGTTGACAGAACTTTTAGAGATATAATGGATAATCCATTACCATTCGGTGGAAAAATGGTTGTGTTAAGAGGAGATTTTCGACAAGTGTTACCTGTTGTCCCACGAGCTACAAGACAACAAACTATTAATGAAAGTTTGGTAAAGTCATACCTTTGGAATAAAATGGAGAAAATCCAACTAACTAAAAATATGAGAGCTCAAAGTGATACCCGTTTTGCAGAATATTTGTTGAAGATTGGAAACAGAACTGAACTGTCAACAGAAGATGATTATATTTGTCTCCCTGCCAATATCATTGTTACAGGTGCAAATGAAGAAGATTCAATATTAAAGTTATTGAATATTGTTTATCCTGATCTCAAAACGCATACTACTTCAGCAGAATACATGACTAATAGAGCAATTCTATCGACAACAAATGAGTATGTTGACCAAATCAATGAAAGAATGATTGAGATGTTTCCTGGAAAAATGGAAGAATTCATGAGTTTTGATGAAGCAATTGATGATACACATAATTATTATCATGAGGAATTTCTCAACTCATTGTTACCTAATGGTGTTCCTCCACACAAATTGTTTCTTAAAAAAGATTGTCCAGTTATACTATTGAGAAACTTAGACCCCAGGCAATGGTCTATGTAA

Protein sequence

MYIKLETTRLDFYKTQQSQIRSELYQGIVDVVNAGETRGDKVGKRVVLPSTFIGGPKNMRHRYLDAMALVQHYGKPDLFITMTCNSEWKEIREELMEGQQSHDRPDLTSRVSRGKLMDLKDQIVHKEIFGNVDNLEKLQHSRNRENIFVSCFVSYYKIKKYDCNCNNNISVAASIMPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKARIKAVDRTFRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLLPNGVPPHKLFLKKDCPVILLRNLDPRQWSM*

Homology

[Close]

BLAST of CSPI03G22280 vs. ExPASy Swiss-Prot
Match: A6ZM04 (ATP-dependent DNA helicase PIF1 OS=Saccharomyces cerevisiae (strain YJM789) OX=307796 GN=PIF1 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-07
Identity = 71/267 (26.59%), Postives = 109/267 (40.82%), Query Frame = 0


Query: 178 GGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKV--IIWDEVPMAKKARIKAVDRT 237
           GG T HS   I L   ++       + ++  L +   +  ++ DE+ M     +  +D  
Sbjct: 298 GGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWENIGALVVDEISMLDAELLDKLDFI 357

Query: 238 FRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEK--IQLTK 297
            R I  N  PFGG  ++  GDF Q+ PV     R     +   +S  W +  K  I L K
Sbjct: 358 ARKIRKNHQPFGGIQLIFCGDFFQLPPVSKDPNRP---TKFAFESKAWKEGVKMTIMLQK 417

Query: 298 NMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPDLKT 357
             R + D +F + L    NR  L   DD              E +   K L+   PD   
Sbjct: 418 VFRQRGDVKFIDML----NRMRLGNIDD--------------ETEREFKKLSRPLPD--D 477

Query: 358 HTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFD-EAIDDTHNYYHEEFLN 417
               AE       L +T   V++ N   +   PG++  F + D  A++D      EE   
Sbjct: 478 EIIPAE-------LYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALED------EELKE 528

Query: 418 SLLPNGVPPHKLFLKKDCPVILLRNLD 440
            LL N + P +L LK    V++++NLD
Sbjct: 538 RLLQNFLAPKELHLKVGAQVMMVKNLD 528

[Close]

BLAST of CSPI03G22280 vs. ExPASy Swiss-Prot
Match: P07271 (ATP-dependent DNA helicase PIF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PIF1 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-07
Identity = 71/267 (26.59%), Postives = 109/267 (40.82%), Query Frame = 0


Query: 178 GGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKV--IIWDEVPMAKKARIKAVDRT 237
           GG T HS   I L   ++       + ++  L +   +  ++ DE+ M     +  +D  
Sbjct: 298 GGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWENIGALVVDEISMLDAELLDKLDFI 357

Query: 238 FRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEK--IQLTK 297
            R I  N  PFGG  ++  GDF Q+ PV     R     +   +S  W +  K  I L K
Sbjct: 358 ARKIRKNHQPFGGIQLIFCGDFFQLPPVSKDPNRP---TKFAFESKAWKEGVKMTIMLQK 417

Query: 298 NMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPDLKT 357
             R + D +F + L    NR  L   DD              E +   K L+   PD   
Sbjct: 418 VFRQRGDVKFIDML----NRMRLGNIDD--------------ETEREFKKLSRPLPD--D 477

Query: 358 HTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFD-EAIDDTHNYYHEEFLN 417
               AE       L +T   V++ N   +   PG++  F + D  A++D      EE   
Sbjct: 478 EIIPAE-------LYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALED------EELKE 528

Query: 418 SLLPNGVPPHKLFLKKDCPVILLRNLD 440
            LL N + P +L LK    V++++NLD
Sbjct: 538 RLLQNFLAPKELHLKVGAQVMMVKNLD 528

[Close]

BLAST of CSPI03G22280 vs. ExPASy Swiss-Prot
Match: A5WFR0 (ATP-dependent DNA helicase pif1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 GN=pif1 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 6.3e-07
Identity = 61/243 (25.10%), Postives = 105/243 (43.21%), Query Frame = 0


Query: 152 FVSYYKIKKYDCNCNNNISVAASIMPGGRTTHSRFNIPL--ETHESSICNISKQSNKTEL 211
           ++ Y + ++       +  +AA+ M  G T HS   I +  E  E  + N+S++    + 
Sbjct: 33  YIHYLRARRVPVATTASTGIAATHM-NGITIHSWSGIGIKDELTERDLVNLSRKKVLKDR 92

Query: 212 IKQAKVIIWDEVPMAKKARIKAVDRTFRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATR 271
           +++  V+I DE+ M    ++  V++  + +  N  PFGG  VV+ GDF Q+ PV  R   
Sbjct: 93  LQETAVLIIDEISMLHAKQLNLVNQVLKHMRQNDKPFGGIQVVVAGDFFQLPPVGSRGES 152

Query: 272 QQTINESLVKSYLWNKMEKIQLTKNMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANI 331
            +     + +++L    +   L++  R QS                   ED  I L  NI
Sbjct: 153 NRDKFAFMSQAWLDAGFKICYLSEQHRQQSG----------------EGEDAQITLD-NI 212

Query: 332 I--VTGAN--EEDSILKLLNIVYPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIEMF 389
           +  + G N     +I  L N  Y D+           NR  L T N  V++INE  + + 
Sbjct: 213 LNQIRGENGVSAAAIAALQNTFYQDVD---------VNRTRLYTHNVNVNKINEHELALL 248

[Close]

BLAST of CSPI03G22280 vs. ExPASy Swiss-Prot
Match: P38766 (ATP-dependent DNA helicase RRM3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RRM3 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.6e-05
Identity = 53/194 (27.32%), Postives = 84/194 (43.30%), Query Frame = 0


Query: 173 ASIMPGGRTTH--SRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKARIK 232
           A++  GG T H  S   I  +T +  +  I  Q +     +  KV+I DE+ M     + 
Sbjct: 289 AAVTIGGSTLHKWSGIGIGNKTIDQLVKKIQSQKDLLAAWRYTKVLIIDEISMVDGNLLD 348

Query: 233 AVDRTFRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNK--MEK 292
            +++  R I  N  PFGG  +VL GDF Q+ PV  +   +  + +   +S +W +   + 
Sbjct: 349 KLEQIARRIRKNDDPFGGIQLVLTGDFFQLPPVAKK--DEHNVVKFCFESEMWKRCIQKT 408

Query: 293 IQLTKNMRAQSD--------TRFAEYLLKIGNR-TELSTEDDYI--CLPANIIVTGANEE 352
           I LTK  R Q +         R+ E  + I      L+ + DY     P  +  T    E
Sbjct: 409 ILLTKVFRQQDNKLIDILNAIRYGELTVDIAKTIRNLNRDIDYADGIAPTELYATRREVE 468

[Close]

BLAST of CSPI03G22280 vs. ExPASy Swiss-Prot
Match: Q5AXT5 (ATP-dependent DNA helicase PIF1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=pif1 PE=3 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 3.8e-04
Identity = 68/264 (25.76%), Postives = 98/264 (37.12%), Query Frame = 0


Query: 179 GRTTHSRFNIPL--ETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKARIKAVDRTF 238
           G T HS   I L  E     +  I K         + KV++ DEV M        ++   
Sbjct: 304 GVTLHSFAGIGLGKEPVPELVKKIKKNQKARNRWLRTKVLVIDEVSMVDGDLFDKLEEIA 363

Query: 239 RDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNMR 298
           R I +N  PFGG  +V+ GDF Q LP VP    ++        S+  +    I LT   R
Sbjct: 364 RLIRNNGRPFGGIQLVVTGDFFQ-LPPVPEGHNREAKFSFAAASWNTSIQHTILLTHVFR 423

Query: 299 AQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYP-DLKTHT 358
            Q D  FA+ L                    N +  G     +I     +  P D     
Sbjct: 424 -QRDPEFADML--------------------NEMRLGKLSPRTIQAFKELSRPLDFHDAL 483

Query: 359 TSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLL 418
            + E    RA        VD  N   +    G   E M+F+    D+      +F   LL
Sbjct: 484 EATELFPTRA-------EVDNANSARMARLSG---ETMTFNAV--DSGTIQDIQFREKLL 533

Query: 419 PNGVPPHKLFLKKDCPVILLRNLD 440
            N + P  + LKK   V+L++N++
Sbjct: 544 SNCMAPQTIHLKKGSQVMLIKNME 533

[Close]

BLAST of CSPI03G22280 vs. ExPASy TrEMBL
Match: A0A6J1DMF2 (ATP-dependent DNA helicase OS=Momordica charantia OX=3673 GN=LOC111022374 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 1.1e-110
Identity = 198/270 (73.33%), Postives = 231/270 (85.56%), Query Frame = 0


Query: 171 VAASIMPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKARIK 230
           VAA IMPGGRT HSRFNIPL+ +ESS+CNISKQSN+ EL++QAK+IIWDE P AKK  I+
Sbjct: 11  VAAXIMPGGRTAHSRFNIPLQANESSMCNISKQSNRAELVRQAKLIIWDEAPXAKKVTIE 70

Query: 231 AVDRTFRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQ 290
           AVDRTF+DIM+N  PFGGK++V  GDFRQVLPVVPRATRQQT++ESLVKSYLW +MEK+Q
Sbjct: 71  AVDRTFQDIMENSKPFGGKVIVFGGDFRQVLPVVPRATRQQTVHESLVKSYLWKQMEKLQ 130

Query: 291 LTKNMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPD 350
           L KNMRAQ+D +FAEYLLKIGN TE S  +DY+ LP +I +     EDS+LKLLNIVYPD
Sbjct: 131 LKKNMRAQNDYQFAEYLLKIGNGTEPSIVEDYVRLPKDIAIIDEGHEDSVLKLLNIVYPD 190

Query: 351 LKTHTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDTHNYYHEEF 410
           L  +  SAEY+TNRAILSTTNEYVD+IN +MI +F G +EEFMSFDE IDDTH+YY EEF
Sbjct: 191 LPQNAMSAEYITNRAILSTTNEYVDEINNKMIRLFLGNIEEFMSFDETIDDTHSYYQEEF 250

Query: 411 LNSLLPNGVPPHKLFLKKDCPVILLRNLDP 441
           LNSLLPNG+PPHKL LK +CP+ILLRNLDP
Sbjct: 251 LNSLLPNGIPPHKLILKINCPIILLRNLDP 280

[Close]

BLAST of CSPI03G22280 vs. ExPASy TrEMBL
Match: A0A5B7A9C3 (ATP-dependent DNA helicase OS=Davidia involucrata OX=16924 GN=Din_021512 PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 3.6e-98
Identity = 276/925 (29.84%), Postives = 358/925 (38.70%), Query Frame = 0


Query: 1    MYIKLETTRLDFYKTQQSQIRSELYQGIVDVVNAGETRGDKVGKRVVLPSTFIGGPKNMR 60
            MYIKLETTRLD+Y+ QQS IR++LYQGIVD V AGETRG++VG+R+VLP++F+GGP++MR
Sbjct: 434  MYIKLETTRLDYYRRQQSNIRADLYQGIVDSVMAGETRGNQVGQRIVLPASFVGGPRDMR 493

Query: 61   HRYLDAMALVQHYGKPDLFITMTCNSEWKEIREELMEGQQSHDRPDLTSRVSRGKLMDLK 120
             RYLDA+ALVQ +GKPDLFITMTCN +WKE+++EL EGQ+  DRPDLTSRV R KL DLK
Sbjct: 494  RRYLDAIALVQRFGKPDLFITMTCNPDWKEVQDELQEGQRPQDRPDLTSRVFRAKLQDLK 553

Query: 121  DQIVHKEIFGNV------------------------------------------------ 180
            DQ+  K IFG V                                                
Sbjct: 554  DQLFKKAIFGKVAAHVYVIEFQKRGLPHAHMLIILKSEYKITNADQYDKFVCAELPDKGK 613

Query: 181  ---------------------------------------------------------DNL 240
                                                                     DN 
Sbjct: 614  YPQLYEMVVKHMMHGPCGEKNPKNPCMVNGECKNHYPRSFCEKTIQGKDGYPIYRRRDNK 673

Query: 241  EKLQ-----------------------------------------------HSR------ 300
            EK+                                                H R      
Sbjct: 674  EKVTVRNAELDNKWVVPYNPYLLTRYNCHINVEICSGLKAVKYLYKYIYKGHDRVAVYIA 733

Query: 301  --NRENI-----------FVSC------------------------------FVSYYK-- 360
              + EN+           +VS                               FV+Y++  
Sbjct: 734  YNDEENVVDEIQQFQDARWVSAQEAMWRIYEFNLNEIYPAVINLQLHLPNKQFVTYWENQ 793

Query: 361  ------------------------------------------------------------ 420
                                                                        
Sbjct: 794  NLEHIICWDHISKTMLTEFFNTCYQKEEARRYLYREFPEHYVWHRQDRCWHERKKGEVIG 853

Query: 421  ------------------------------------------------------------ 441
                                                                        
Sbjct: 854  RINGANPMEGERYYLRLLLNHVRGPTSFEDLLTADGIRYLTFKESAQKRGLLESDNSISE 913

[Close]

BLAST of CSPI03G22280 vs. ExPASy TrEMBL
Match: A0A151SKD2 (ATP-dependent DNA helicase OS=Cajanus cajan OX=3821 GN=KK1_001464 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 6.2e-98
Identity = 183/440 (41.59%), Postives = 276/440 (62.73%), Query Frame = 0


Query: 2   YIKLETTRLDFYKTQQSQIRSELYQGIVDVVNAGETRGDKVGKRVVLPSTFIGGPKNMRH 61
           Y+K+E  RL + +  Q+ IR+E+YQG+ D ++ GET  + +G+R +LPS+FIG  +++  
Sbjct: 166 YVKIELGRLRWIRNHQNNIRAEVYQGLQDALHEGETMRENIGQRTILPSSFIGSRRDLTQ 225

Query: 62  RYLDAMALVQHYGKPDLFITMTCNSEWKEIREELMEGQQSHDRPDLTSRVSRGKLMDLKD 121
           RY D MA+V + GKPD+F+TMTCN  W EI  +L   Q   DR DL +R+ R K   LK+
Sbjct: 226 RYEDGMAIVLNDGKPDIFLTMTCNPSWSEISSQLGPHQTPPDRLDLLTRIFRSKFGQLKE 285

Query: 122 QIVHKEIFGNVDNLEKLQHSRNRENIFVSCFVSYYKIKKYDCNCNNNISVAASIMPGGRT 181
            +++K + G V            +       ++  + K        +  +AA+++PGGRT
Sbjct: 286 DVINKGVIGKVKR-------GTGKTFLYRTLIANVRSKGQIVLATASSGIAATLLPGGRT 345

Query: 182 THSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKARIKAVDRTFRDIMD 241
            HSRF IP+     S C+ISKQS+  +LI++   IIWDE PM  +  ++A+DR+ +D++D
Sbjct: 346 AHSRFKIPINVEADSFCSISKQSDLAKLIRETTAIIWDEAPMTNRYALEALDRSLKDLLD 405

Query: 242 NPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNMRAQSDT 301
              PFGGK+++L GDFRQVLPVVP+ T+ Q I   +VKS+LW   + + L +NMR+  D 
Sbjct: 406 CNAPFGGKIMILGGDFRQVLPVVPKGTKAQMIYACIVKSHLWPITKVLHLRQNMRSLQDP 465

Query: 302 RFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHTTSAEYM 361
            FAEYL++IG+  E +  DD + +P  I +T    E SI KL+   +P L++HT +A YM
Sbjct: 466 NFAEYLMRIGDGNEPTKLDDMVKVPHQIEITWEG-ESSIQKLIRDTFPQLESHTWNASYM 525

Query: 362 TNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLLPNGVPP 421
             +AIL+  NE V ++N+ +I  F G+    +SFDE   DTHN Y +E+L+S+ P G PP
Sbjct: 526 VEKAILTPKNEDVQKLNDIIINHFLGEERHLLSFDEVEGDTHNLYQQEYLHSITPGGFPP 585

Query: 422 HKLFLKKDCPVILLRNLDPR 442
           H L +KK   ++LLRN+DP+
Sbjct: 586 HNLKVKKYALLMLLRNIDPK 597

[Close]

BLAST of CSPI03G22280 vs. ExPASy TrEMBL
Match: A0A1D6Q7N5 (ATP-dependent DNA helicase OS=Zea mays OX=4577 GN=ZEAMMB73_Zm00001d051540 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.8e-97
Identity = 191/441 (43.31%), Postives = 273/441 (61.90%), Query Frame = 0


Query: 2   YIKLETTRLDFYKTQQSQIRSELYQGIVDVVNAGETRGDKVGKRVVLPSTFIGGPKNMRH 61
           YIK+E++RLDF +  Q ++R++LY+G+VD ++ GE R DK+GKR VL ++FIGGP++MRH
Sbjct: 104 YIKIESSRLDFIRKNQDKLRADLYKGLVDSLHEGENRADKIGKRTVLSTSFIGGPRDMRH 163

Query: 62  RYLDAMALVQHYGKPDLFITMTCNSEWKEIREELMEGQQSHDRPDLTSRVSRGKLMDLKD 121
           RY+DAMALV+ +GKPD+F+TMTCN  W EI  EL+  Q   D PDL  R+   KL +LK 
Sbjct: 164 RYMDAMALVRKFGKPDIFLTMTCNPNWDEITRELLPMQSPRDCPDLVVRIFHAKLEELKK 223

Query: 122 QIVHKEIFGNVDNLEKLQHSRNRENIFVSCFVSYYKIKKYDCNCNNNISVAASI-MPGGR 181
           ++  + I G                            +KY   C+    +  S  +P  +
Sbjct: 224 RLTKQNILGK---------------------------RKYKLTCHEQYDLLISAEIPDKK 283

Query: 182 TTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKARIKAVDRTFRDIM 241
                + +           + K    +++ K+A +IIWDE  M K+  I+A+D + RDIM
Sbjct: 284 KYPQLYKM-----------LHKTKRDSKVAKKASLIIWDEASMTKRQAIEALDNSMRDIM 343

Query: 242 D-NPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNMRAQS 301
             + LPFGGK VV RGDFRQVLPVV + +R Q +  SL  SYLW  M  ++L +NMRAQS
Sbjct: 344 GRSVLPFGGKTVVFRGDFRQVLPVVRKGSRAQIVAASLRSSYLWESMCHLKLVQNMRAQS 403

Query: 302 DTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHTTSAE 361
           D  FAEYLL++G+ T+ S  D  + LP  + V     +  + +L++ +YP+L  + ++  
Sbjct: 404 DPWFAEYLLRLGDGTKESNNDGDVRLPDEVCVPYTGNDRDLDRLIDDIYPNLNENMSNTS 463

Query: 362 YMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLLPNGV 421
           Y+T+RAILST N++VD IN RMI+ F G+   + SFD A+DD +NYY  EFLN+L PN +
Sbjct: 464 YITSRAILSTWNDWVDMINMRMIDRFQGEQMMYHSFDTAVDDPNNYYPSEFLNTLTPNRL 506

Query: 422 PPHKLFLKKDCPVILLRNLDP 441
           PPH L LK  CP++LLRN+DP
Sbjct: 524 PPHVLKLKVGCPIMLLRNVDP 506

[Close]

BLAST of CSPI03G22280 vs. ExPASy TrEMBL
Match: A0A6J1DFU4 (ATP-dependent DNA helicase OS=Momordica charantia OX=3673 GN=LOC111020055 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 1.4e-94
Identity = 173/218 (79.36%), Postives = 194/218 (88.99%), Query Frame = 0


Query: 223 MAKKARIKAVDRTFRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYL 282
           MAKK  I+AVDRTFRDIMD+  PFGGK+VV  GDFRQVLPVVPRATRQQTINESLVKSYL
Sbjct: 1   MAKKTTIEAVDRTFRDIMDSTEPFGGKIVVFGGDFRQVLPVVPRATRQQTINESLVKSYL 60

Query: 283 WNKMEKIQLTKNMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILK 342
           W +MEK+QLTKNMRA SD++FAEYLLKIGN TE S EDDYICL  +I +TG N+E +I+K
Sbjct: 61  WKQMEKLQLTKNMRAHSDSQFAEYLLKIGNGTEPSVEDDYICLTDDITITGENDEGAIMK 120

Query: 343 LLNIVYPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDT 402
           LLN+VYPDL+ + TS+EYMTNR ILSTTNEYVDQINE+MI++FPGK  EFMSFDEAIDDT
Sbjct: 121 LLNVVYPDLEQNATSSEYMTNRVILSTTNEYVDQINEKMIQLFPGKFFEFMSFDEAIDDT 180

Query: 403 HNYYHEEFLNSLLPNGVPPHKLFLKKDCPVILLRNLDP 441
           HNYY EEFLNSLLPNG+PPHKL LK +CPVILLRNLDP
Sbjct: 181 HNYYQEEFLNSLLPNGIPPHKLMLKANCPVILLRNLDP 218

[Close]

BLAST of CSPI03G22280 vs. NCBI nr
Match: XP_031737538.1 (uncharacterized protein LOC116402430 [Cucumis sativus])

HSP 1 Score: 452.2 bits (1162), Expect = 5.2e-123
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0


Query: 223 MAKKARIKAVDRTFRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYL 282
           MAKKARIKAVDRTFRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYL
Sbjct: 1   MAKKARIKAVDRTFRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYL 60

Query: 283 WNKMEKIQLTKNMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILK 342
           WNKMEKIQLTKNMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILK
Sbjct: 61  WNKMEKIQLTKNMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILK 120

Query: 343 LLNIVYPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDT 402
           LLNIVYPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDT
Sbjct: 121 LLNIVYPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDT 180

Query: 403 HNYYHEEFLNSLLPNGVPPHKLFLKKDCPVILLRNLDPRQWSM 446
           HNYYHEEFLNSLLPNGVPPHKLFLKKDCPVILLRNLDPRQWSM
Sbjct: 181 HNYYHEEFLNSLLPNGVPPHKLFLKKDCPVILLRNLDPRQWSM 223

[Close]

BLAST of CSPI03G22280 vs. NCBI nr
Match: XP_022155233.1 (ATP-dependent DNA helicase PIF1-like [Momordica charantia])

HSP 1 Score: 410.2 bits (1053), Expect = 2.2e-110
Identity = 198/270 (73.33%), Postives = 231/270 (85.56%), Query Frame = 0


Query: 171 VAASIMPGGRTTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKARIK 230
           VAA IMPGGRT HSRFNIPL+ +ESS+CNISKQSN+ EL++QAK+IIWDE P AKK  I+
Sbjct: 11  VAAXIMPGGRTAHSRFNIPLQANESSMCNISKQSNRAELVRQAKLIIWDEAPXAKKVTIE 70

Query: 231 AVDRTFRDIMDNPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQ 290
           AVDRTF+DIM+N  PFGGK++V  GDFRQVLPVVPRATRQQT++ESLVKSYLW +MEK+Q
Sbjct: 71  AVDRTFQDIMENSKPFGGKVIVFGGDFRQVLPVVPRATRQQTVHESLVKSYLWKQMEKLQ 130

Query: 291 LTKNMRAQSDTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPD 350
           L KNMRAQ+D +FAEYLLKIGN TE S  +DY+ LP +I +     EDS+LKLLNIVYPD
Sbjct: 131 LKKNMRAQNDYQFAEYLLKIGNGTEPSIVEDYVRLPKDIAIIDEGHEDSVLKLLNIVYPD 190

Query: 351 LKTHTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDTHNYYHEEF 410
           L  +  SAEY+TNRAILSTTNEYVD+IN +MI +F G +EEFMSFDE IDDTH+YY EEF
Sbjct: 191 LPQNAMSAEYITNRAILSTTNEYVDEINNKMIRLFLGNIEEFMSFDETIDDTHSYYQEEF 250

Query: 411 LNSLLPNGVPPHKLFLKKDCPVILLRNLDP 441
           LNSLLPNG+PPHKL LK +CP+ILLRNLDP
Sbjct: 251 LNSLLPNGIPPHKLILKINCPIILLRNLDP 280

[Close]

BLAST of CSPI03G22280 vs. NCBI nr
Match: KYP55257.1 (hypothetical protein KK1_001464 [Cajanus cajan])

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-97
Identity = 183/440 (41.59%), Postives = 276/440 (62.73%), Query Frame = 0


Query: 2   YIKLETTRLDFYKTQQSQIRSELYQGIVDVVNAGETRGDKVGKRVVLPSTFIGGPKNMRH 61
           Y+K+E  RL + +  Q+ IR+E+YQG+ D ++ GET  + +G+R +LPS+FIG  +++  
Sbjct: 166 YVKIELGRLRWIRNHQNNIRAEVYQGLQDALHEGETMRENIGQRTILPSSFIGSRRDLTQ 225

Query: 62  RYLDAMALVQHYGKPDLFITMTCNSEWKEIREELMEGQQSHDRPDLTSRVSRGKLMDLKD 121
           RY D MA+V + GKPD+F+TMTCN  W EI  +L   Q   DR DL +R+ R K   LK+
Sbjct: 226 RYEDGMAIVLNDGKPDIFLTMTCNPSWSEISSQLGPHQTPPDRLDLLTRIFRSKFGQLKE 285

Query: 122 QIVHKEIFGNVDNLEKLQHSRNRENIFVSCFVSYYKIKKYDCNCNNNISVAASIMPGGRT 181
            +++K + G V            +       ++  + K        +  +AA+++PGGRT
Sbjct: 286 DVINKGVIGKVKR-------GTGKTFLYRTLIANVRSKGQIVLATASSGIAATLLPGGRT 345

Query: 182 THSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKARIKAVDRTFRDIMD 241
            HSRF IP+     S C+ISKQS+  +LI++   IIWDE PM  +  ++A+DR+ +D++D
Sbjct: 346 AHSRFKIPINVEADSFCSISKQSDLAKLIRETTAIIWDEAPMTNRYALEALDRSLKDLLD 405

Query: 242 NPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNMRAQSDT 301
              PFGGK+++L GDFRQVLPVVP+ T+ Q I   +VKS+LW   + + L +NMR+  D 
Sbjct: 406 CNAPFGGKIMILGGDFRQVLPVVPKGTKAQMIYACIVKSHLWPITKVLHLRQNMRSLQDP 465

Query: 302 RFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHTTSAEYM 361
            FAEYL++IG+  E +  DD + +P  I +T    E SI KL+   +P L++HT +A YM
Sbjct: 466 NFAEYLMRIGDGNEPTKLDDMVKVPHQIEITWEG-ESSIQKLIRDTFPQLESHTWNASYM 525

Query: 362 TNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLLPNGVPP 421
             +AIL+  NE V ++N+ +I  F G+    +SFDE   DTHN Y +E+L+S+ P G PP
Sbjct: 526 VEKAILTPKNEDVQKLNDIIINHFLGEERHLLSFDEVEGDTHNLYQQEYLHSITPGGFPP 585

Query: 422 HKLFLKKDCPVILLRNLDPR 442
           H L +KK   ++LLRN+DP+
Sbjct: 586 HNLKVKKYALLMLLRNIDPK 597

[Close]

BLAST of CSPI03G22280 vs. NCBI nr
Match: AQK54472.1 (hypothetical protein ZEAMMB73_Zm00001d051540 [Zea mays])

HSP 1 Score: 366.3 bits (939), Expect = 3.7e-97
Identity = 191/441 (43.31%), Postives = 273/441 (61.90%), Query Frame = 0


Query: 2   YIKLETTRLDFYKTQQSQIRSELYQGIVDVVNAGETRGDKVGKRVVLPSTFIGGPKNMRH 61
           YIK+E++RLDF +  Q ++R++LY+G+VD ++ GE R DK+GKR VL ++FIGGP++MRH
Sbjct: 104 YIKIESSRLDFIRKNQDKLRADLYKGLVDSLHEGENRADKIGKRTVLSTSFIGGPRDMRH 163

Query: 62  RYLDAMALVQHYGKPDLFITMTCNSEWKEIREELMEGQQSHDRPDLTSRVSRGKLMDLKD 121
           RY+DAMALV+ +GKPD+F+TMTCN  W EI  EL+  Q   D PDL  R+   KL +LK 
Sbjct: 164 RYMDAMALVRKFGKPDIFLTMTCNPNWDEITRELLPMQSPRDCPDLVVRIFHAKLEELKK 223

Query: 122 QIVHKEIFGNVDNLEKLQHSRNRENIFVSCFVSYYKIKKYDCNCNNNISVAASI-MPGGR 181
           ++  + I G                            +KY   C+    +  S  +P  +
Sbjct: 224 RLTKQNILGK---------------------------RKYKLTCHEQYDLLISAEIPDKK 283

Query: 182 TTHSRFNIPLETHESSICNISKQSNKTELIKQAKVIIWDEVPMAKKARIKAVDRTFRDIM 241
                + +           + K    +++ K+A +IIWDE  M K+  I+A+D + RDIM
Sbjct: 284 KYPQLYKM-----------LHKTKRDSKVAKKASLIIWDEASMTKRQAIEALDNSMRDIM 343

Query: 242 D-NPLPFGGKMVVLRGDFRQVLPVVPRATRQQTINESLVKSYLWNKMEKIQLTKNMRAQS 301
             + LPFGGK VV RGDFRQVLPVV + +R Q +  SL  SYLW  M  ++L +NMRAQS
Sbjct: 344 GRSVLPFGGKTVVFRGDFRQVLPVVRKGSRAQIVAASLRSSYLWESMCHLKLVQNMRAQS 403

Query: 302 DTRFAEYLLKIGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHTTSAE 361
           D  FAEYLL++G+ T+ S  D  + LP  + V     +  + +L++ +YP+L  + ++  
Sbjct: 404 DPWFAEYLLRLGDGTKESNNDGDVRLPDEVCVPYTGNDRDLDRLIDDIYPNLNENMSNTS 463

Query: 362 YMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFDEAIDDTHNYYHEEFLNSLLPNGV 421
           Y+T+RAILST N++VD IN RMI+ F G+   + SFD A+DD +NYY  EFLN+L PN +
Sbjct: 464 YITSRAILSTWNDWVDMINMRMIDRFQGEQMMYHSFDTAVDDPNNYYPSEFLNTLTPNRL 506

Query: 422 PPHKLFLKKDCPVILLRNLDP 441
           PPH L LK  CP++LLRN+DP
Sbjct: 524 PPHVLKLKVGCPIMLLRNVDP 506

[Close]

BLAST of CSPI03G22280 vs. NCBI nr
Match: KAG5560632.1 (hypothetical protein RHGRI_003828 [Rhododendron griersonianum])

HSP 1 Score: 363.6 bits (932), Expect = 2.4e-96
Identity = 254/820 (30.98%), Postives = 343/820 (41.83%), Query Frame = 0


Query: 1    MYIKLETTRLDFYKTQQSQIRSELYQGIVDVVNAGETRGDKVGKRVVLPSTFIGGPKNMR 60
            MYIKLE TRLD+++ +QS IR+ELYQGIVD V  GE+RG  VGK++VLP++F+GGP++MR
Sbjct: 240  MYIKLENTRLDYFRHKQSDIRAELYQGIVDSVLGGESRGSMVGKKIVLPASFVGGPRDMR 299

Query: 61   HRYLDAMALVQHYGKPDLFITMTCNSEWKEIREELMEGQQSHDRPDLTSRVSRGKLMDLK 120
             RYLDA++LVQ +GKPDLFITMTCN +WKEI++ L +GQ + DRPDLT+RV R KL DLK
Sbjct: 300  RRYLDAISLVQKFGKPDLFITMTCNPDWKEIKDYLKDGQLAQDRPDLTARVFRAKLKDLK 359

Query: 121  DQIVHKEIFGNV------------------------------------------------ 180
             Q+  K IFG V                                                
Sbjct: 360  YQLFKKHIFGKVAAHVHVIEFQKRGLPHAHMLIILKPECKITSPDHFDKFVCAEIPDPDK 419

Query: 181  -------------------------------------------------DNLEKLQHSR- 240
                                                             D +++ Q +R 
Sbjct: 420  FPDLYEKVAKHMMHGPCGVKAVKYLYKYIYKGHDKVTVHISNNVGDITIDEIQQFQDARW 479

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 480  VSAQEAIWRIFEFELNEMYPAVINLQLHLPNKQFVSYWANQDLNNIIHSDTASRTMLTEF 539

Query: 301  ------------------------NRE--------------------------------- 360
                                    NR                                  
Sbjct: 540  FNFCSKDKEKRKYLYREFPEYFVWNRNGKCWEERKTREALTSFADLLMYNDVQCSSFKEA 599

Query: 361  ----------------------------------NIFVSC-------------------- 420
                                               + V C                    
Sbjct: 600  AERRGLLQSDDWIIESLLEAASFKMPLALRQLFATVLVYCEPSDVRKLWDLLFEAMSEDF 659

Query: 421  -----------------FVSYY------KIKKYDC------------------------- 441
                              V Y+       IK YD                          
Sbjct: 660  TRKEAVSTEVCLLKTLRSVDYFLDGMGRSIKDYDLPSMMSNICETEFIGFREVLDELQIR 719

[Close]

BLAST of CSPI03G22280 vs. TAIR 10
Match: AT3G51690.1 (PIF1 helicase )

HSP 1 Score: 62.8 bits (151), Expect = 8.2e-10
Identity = 44/133 (33.08%), Postives = 72/133 (54.14%), Query Frame = 0


Query: 310 IGNRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHTTSAEYMTNRAILST 369
           I N  +  TE D   +  ++++T    +D I  LL  VY +    + + ++  + AIL  
Sbjct: 71  INNPNDGETEID---ISKDLLIT--ESKDPIKTLLKEVYGEYFAKSYNPDFCHDSAILCH 130

Query: 370 TNEYVDQINERMIEMFPGKMEEFMSFDEAIDDTHN--YYHEEFLNSLLPNGVPPHKLFLK 429
            ++ VDQIN+ M+ + PG+ +E +S D      ++  +   E LNS+   G+P  KL LK
Sbjct: 131 RDDDVDQINDYMLSLLPGEEKECLSTDSISPSPNDDMFVPLEVLNSIKVPGLPDFKLRLK 190

Query: 430 KDCPVILLRNLDP 441
              PV+LLR+LDP
Sbjct: 191 VGAPVMLLRDLDP 198

[Close]

BLAST of CSPI03G22280 vs. TAIR 10
Match: AT3G51700.1 (PIF1 helicase )

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-09
Identity = 45/151 (29.80%), Postives = 75/151 (49.67%), Query Frame = 0


Query: 300 DTRFAEYLLKIG--NRTELSTEDDYICLPANIIVTGANEEDSILKLLNIVYPDLKTHTTS 359
           D  F +++  IG  N  + +  +  I +  ++++T    +D I  +++ VY +  T + +
Sbjct: 57  DEAFTKWITNIGGENINKPNDGETKIDIHEDLLITEC--KDPIKTIVDEVYGESFTESYN 116

Query: 360 AEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMSFD-----EAIDDTHNYYHEEFLN 419
            ++   RAIL  TN+  D+IN+ M+    G+  +    D      A  +    Y  EFLN
Sbjct: 117 PDFYQERAILCHTNDVADEINDYMLSQLQGEETKCYGADTIYPTHASPNDKMLYPLEFLN 176

Query: 420 SLLPNGVPPHKLFLKKDCPVILLRNLDPRQW 444
           S+   G P  KL LK   PV+LLR+L P  W
Sbjct: 177 SIKIPGFPDFKLRLKVGAPVMLLRDLAPYGW 205

[Close]

BLAST of CSPI03G22280 vs. TAIR 10
Match: AT5G28780.1 (PIF1 helicase )

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-09
Identity = 34/101 (33.66%), Postives = 53/101 (52.48%), Query Frame = 0


Query: 348 YPDLKTHTTSAEYMTNRAILSTTNEYVDQINERMIEMFPGKMEEFMS------FDEAIDD 407
           Y        + +Y+T R IL+  NEYVD+IN  M+    G  +E++S       D    D
Sbjct: 102 YVQFSVSQPNFQYLTERGILTPHNEYVDEINAYMLSQVGGDSKEYLSSYSIGKADTIGAD 161

Query: 408 THNYYHEEFLNSLLPNGVPPHKLFLKKDCPVILLRNLDPRQ 443
               YH ++LNSL    +P HK+ LKK  P++ +RN + ++
Sbjct: 162 YEALYHVKYLNSLEFPSLPKHKISLKKGVPIMQMRNFNQKE 202

The following BLAST results are available for this feature:

BLAST of CSPI03G22280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A6ZM04	1.3e-07	26.59	ATP-dependent DNA helicase PIF1 OS=Saccharomyces cerevisiae (strain YJM789) OX=3...	[more]
P07271	1.3e-07	26.59	ATP-dependent DNA helicase PIF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 ...	[more]
A5WFR0	6.3e-07	25.10	ATP-dependent DNA helicase pif1 OS=Psychrobacter sp. (strain PRwf-1) OX=349106 G...	[more]
P38766	1.6e-05	27.32	ATP-dependent DNA helicase RRM3 OS=Saccharomyces cerevisiae (strain ATCC 204508 ...	[more]
Q5AXT5	3.8e-04	25.76	ATP-dependent DNA helicase PIF1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38...	[more]

BLAST of CSPI03G22280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DMF2	1.1e-110	73.33	ATP-dependent DNA helicase OS=Momordica charantia OX=3673 GN=LOC111022374 PE=3 S...	[more]
A0A5B7A9C3	3.6e-98	29.84	ATP-dependent DNA helicase OS=Davidia involucrata OX=16924 GN=Din_021512 PE=3 SV...	[more]
A0A151SKD2	6.2e-98	41.59	ATP-dependent DNA helicase OS=Cajanus cajan OX=3821 GN=KK1_001464 PE=3 SV=1	[more]
A0A1D6Q7N5	1.8e-97	43.31	ATP-dependent DNA helicase OS=Zea mays OX=4577 GN=ZEAMMB73_Zm00001d051540 PE=3 S...	[more]
A0A6J1DFU4	1.4e-94	79.36	ATP-dependent DNA helicase OS=Momordica charantia OX=3673 GN=LOC111020055 PE=3 S...	[more]

BLAST of CSPI03G22280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031737538.1	5.2e-123	100.00	uncharacterized protein LOC116402430 [Cucumis sativus]	[more]
XP_022155233.1	2.2e-110	73.33	ATP-dependent DNA helicase PIF1-like [Momordica charantia]	[more]
KYP55257.1	1.3e-97	41.59	hypothetical protein KK1_001464 [Cajanus cajan]	[more]
AQK54472.1	3.7e-97	43.31	hypothetical protein ZEAMMB73_Zm00001d051540 [Zea mays]	[more]
KAG5560632.1	2.4e-96	30.98	hypothetical protein RHGRI_003828 [Rhododendron griersonianum]	[more]

BLAST of CSPI03G22280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51690.1	8.2e-10	33.08	PIF1 helicase	[more]
AT3G51700.1	1.1e-09	29.80	PIF1 helicase	[more]
AT5G28780.1	1.1e-09	33.66	PIF1 helicase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010285	DNA helicase Pif1-like	PFAM	PF05970	PIF1	coord: 170..441 e-value: 3.7E-81 score: 273.1
IPR025476	Helitron helicase-like domain	PFAM	PF14214	Helitron_like_N	coord: 1..134 e-value: 5.5E-31 score: 108.2
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 149..295 e-value: 1.8E-13 score: 52.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 244..440
None	No IPR available	PANTHER	PTHR10492:SF76	ATP-DEPENDENT DNA HELICASE	coord: 170..441 coord: 2..139
None	No IPR available	PANTHER	PTHR10492	UNCHARACTERIZED	coord: 170..441 coord: 2..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G22280.1	CSPI03G22280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032508	DNA duplex unwinding
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006281	DNA repair
biological_process	GO:0000723	telomere maintenance
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0003678	DNA helicase activity