CSPI03G22250 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G22250
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	2-isopropylmalate synthase
Location	Chr3: 18695414 .. 18706018 (+)
RNA-Seq Expression	CSPI03G22250
Synteny	CSPI03G22250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAGAAAAAGAATAAATGTTTTATTCACCGGTTCCGTTCCGGGTTGAAAGCCCAAAAATCAACTGAAAAACCCATTTTTTGGAAAAATATCAAATGACTTTCTGTCACGGACACGTGCAGTTACGAATTTACCCCTTTTGTTTTTTCCTTTTCACTTCCCTGCTTCTTCTCCGACGAGTGTTCAATATGCGACAGTTGAAGTCTCAGGGTCCCCTCACCGGCATTATATATTATTAGTATGACCTAAACCTCAAGCCACTCACAAACGTCCACTGTAGCTCGCCTCTAAGTAAGTCTTCCCTGTTCTTTATTCATTCCAGATTAATTCCTTCCTCACTTCCTCTCTCACCGTTACCCCTCTGTACAATACAAGTCCATTTCTGTTTTCTTTGATTTCCTTTGTTCTCTTTACCATGGCCCTTCCGGCAGCATTGTCCAGACCCAATCATTCTCTTGCTCGCCAATCCGATTCTCTGTTTCCTGCTCCTCATCGGATTTATAGCAGGAATTTGCAGGTTCTTAAGAATCCTTCACGGTCGTTGTCTTTTAAAACGGCTGCTGCTTCGGCGCCTCATAATGTATCTCCTGTTTACTCTTGTCTTACGGAAAGTATTGTTTCGAATTCTGTACCGAAATCATCAATTAGACGGCCGCCGTATATTCCTAATCGCATCCCTGACCCTTCCTATGTTCGCGTATTCGATACCACTCTCCGTGATGGCGAGCAGTCTCCAGGTGCTTCCCTCACTGTTAAAGAGAAGCTTGACATTGCTCGTCAGCTTGCAAAGCTTGGTGTAGATATCATTGAGGCTGGATTCCCTGCTGCTTCGAAGGAAGACTTTGAGGCAGTCAAGATGATTGCAAAAGAGATTGGAAATGCTGTCGATGAGGATGGATATGTGCCTGTTATTTGTGGTCTCTCCAGGTGTAATGAGAAGGATATTCGAACTGCATGGGAGGCTGTCAAATACGCTAAAAGACCGCGGATACATACCTTTATTGCAACGAGCGAAATTCATATGGAGCATAAGCTGAGAAAAACGAAGGAGGAAGTGATTGAAATTGCTCGAAACATGGTGCGGTTTGCACGAAGTTTGGGTTGTGATGATGTTGAGTTCAGCCCCGAGGATGCTGGCAGGTTTGATCTTGATCTTGAGTTTTATGCAATTTTTAAATTTTGTCAACGAATTGATCCAGAGAGTAAGTTGACTCTGCATGCTCCTTGGATTTCAAAACCTCTCAGTCATAATGGATGCTTGGATTAAATTTTTAATAAGCTTAGATTATGTTGGAAGTTGTATGGTGTGAATTTGTATAACTTGTTTCTGAGTATAAACGAGTAGGATACACAAGTTTAAATTTGGGTTATAAAAGGTGCTTGTCAGTTTCCCGGGCCATGTTTGATTTATGTAACTGTATCTGTGTAGCTAATAATGAGCAAATCATTAGTTTTGGTTAGCAGACTCGTATTCTCAATAGGTTGGACTCCCCGCATTACGCAAACTTAACCAATTTTTCTTGACATTTAGACTATAGCATCCTGTTATAACATAGCCTCAATTTGAAGTGGATTGATTTAATCCATTTTTTTGGTTTCCATCATTTAGAATTGTTATAGTAGGTTTTGATGGAAATTTTATTATTATTTATGTGTATGTCTTATGTTCATTGGGCAATCTCCGACTAATGTCTATCCCCATCCTTTTCTTGTTTGGAGCATATCTTTCGTAAGACCCCTTCTTCATGGTGGTTTTCAACATCCTTATCCATTAACAATTGCTTAACATAAATTACAGTTCTTTTAAAGTGATAAAACATTTTTCCTTTTTATGTTGTGGTTTATGATCAGATCTGACAGAGAGTTCCTTTATCAAATTCTGGGGGAAGTGATAAAGGCTGGGGCAACTACTCTCAACATACCTGATACTGTTGGTTATACAATGCCTTTTGAATTTGGGAAGTTGATTGCTGATATAAAATCTAATACTCCTGGCATTGAAAATGTCATCATTTCATCACACTGCCAGAATGATCTTGGACTAGCAACTGCTAACACAGTAGCAGTAAGTAATGATGTTTTCTTCTGCTTCTTCTTTTTTTTTCTATATATTGAACTGGACCTTTAATTTGGAAGTATCCGAAGGTAATAGGATGGTAACAAGGAATGATTGACATACTATTAAAAAACTGCAAGACATGATAAGTAGGATATGGACACACAATGGACATAGTGACACAACTTACTAAATGTAGGGGAGAAAAAAGAAACAAGACATGAATGTGACTTTTATCATATTTTAAGGATGTATAGGCTTGATACTTTACATTGTAAATTATGTAGCAGGGATTAGATGGCACTTTTTTTTGGCTTGATCTTGGTTTAAATTGTTGAAGCTTTTCTGTAATCATTTCCCAAATTACACAAAGCATGAAGTCAAAACTTCTTCAGCCAGTCCTTAGAATTGGAAATTTAAATTTGCACAACGGGAGTTTTGTGTGAACTATGAAAAAATCACTGTTCTGGTTAAATGACGCTGAACCTCAGCTTGTGCCTGTTTTAAATTACGTTTGACCCTTTTCATCTCTTTACAGGGGGCATGTGCGGGTGCAAGGCAAGTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCTTCCCTAGAGGAAGTAAGCTATTCGTTTATTTAATTTATTTTTCCTATGTTTATTTCTTTTGTTGTATAATTATTACATTTATTTTTATTCAATTCTCTGAAGGTTGGGGATAGATTTGCAATACAACCTAACATGATTGATCTAATCAATATGTTATACTTATGAATACACTCCCCCTCACTTGTGGGTTTGAAAATATCAAGAAATACCCAACAAATGGAAATCAATAATAAATGGAGAGAAAATAATGTTGCAAGGGCTTGAACACGTGACCTTCCTGGACCACCTTCTTTTATACCCATCTTAAATCACCAATTCAACCCAAAAGCTTAAGCTTATGGGTGAAGACAAATTTAATACAATATCTAACAGTGTGAACTCTTGCCAAGTAAAACACCTCTCTTTGGCCATTTGTGACTAAAGATTCTTTTGTAACATTGTTTAACCCTTATTATTTTGGGTTAGAGACCCTTTTTATTATGTTAGATCCTTAGATGCTGGTTTGATTCCATTCTTATTGGCGATATAATATTAAAAAGAGGATAAATCTTAAATCTAGAGAAACTAGGGTGTTTATATTTCATTTTGCATGTGACTGAATGCACGTTATGTATTGTTTTGAGAACACCTTTTTGCATTGAATGTAGACACTCATATAGAGTCATCTTGTGTGGGTAGACTTCTTGGCATGCATGAATATGTGGTCATTTTCAATTATGAAAACTTCTTTTATTATCTTCAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATACTGGAATTAACTCAAGACATATCTTTTTGACTAGCAAGATGGTAATTATTTTTCTTACAAAAAATGCTTCCTGAAAAAATTTCGTTGAGTTGTTATTTTTTAAAATTATTCTTACCATTTTCTTAAATTTCAAAATACAACGTTAAACTTTCCTTGGGGTGGGAGATTTGTTGCAAGTTGTTGTTCTTGCTTCTGGTTCCTCTTTAATTTGGAAGAATATGGAGCCAATTTTTTTCAGGAAATGAAGAGGAACATCTCTCTTTGGTGGGTGGGGGTCAGCCACCAGAGTTCAAAATTTTATTTGTTCTATTTGTTTTAGTTGTTTTTCCCCTTTTCTAATTTTGTTTTCGTTTTTTTTTTGCTTTATTATCGTAAAGGCTACTTACTGGCAAAATTCTTCCTTAGGTAGAAGAGTTCACTGGTTTGAATGTACAACCACACAAGGCTATTGTCGGAGCTAATGCTTTTGCTCATGCAAGTGGCATCCATCAGGTTAGTCTTGTTGGATGTTGATATCAAGGTTTTGGCATTCATGGCTTACTATTTATGACAGACATATTTATCATCGTATCATCGTGATGAGATAGCTAGCTAGCCAATTTACTTTATAGTTGACTGTGATCTGATTCTAGTACTAGTGGCCCCTAAAGAAAACCAAATACAACTCTAGAAGCTTAGAAAAAAACCCAACCAAAAATGCCAAGAACTCAGACTCCAACAAGGGAACAACCAGAAGGTAAACAAAAATCCTGAAAGCACAACCAAAATAAAACTCTTAATAAGATGCGATAAGCACTGTTCCAGAACCTAGAAACAAAATGCGGAAGGAAAAAACTTCAGAAGAAATTAAAAGCGTTGAGGAAGTATCACCTATTACTATATTGCATTTAAAGCAAACAAAAGATCTTTGTTTTTTTTTTCCTTTACTTCCCATTTCCCTGTGAGTTGATTGGGAAAAGGAATATATTATAGATAATGAAATTCAGGAAACGAAGGTACAAGAAGGGATGGTAATGAGGGATTAGCCTTTCCTACAAGGCGATGGAAGTTGCATTCTACTAAACTCTTTCCAATTTGATAGAATTAGGAGCGTGAGTAACCTTCTGTTAAGCACTCAAAGAGGAAGGAAGAAATACTGTCGCATATAGTGTCAAAATTTCTATCCATGAGTAACCATCTTGAAGATAGCCCTGGCTACACTTGCAGCTTTTTGCCTCATAGGGTGACCTAGCACTTGAGCCAAAATTACTGTCCATCCGAAAGCTCTCAACTGGGTGTACCTTGTAGTTTTATCTTTTCCTTCATCATTCCACCTGAGTCGTCTGAGTATTTTTGGGGCTGCGAAATGTATATTATTGGTTTTAAAGGACTGGTTGTGCATCATTAATTTTGGGGAGTTGATGCGGTTGTTATACAGCTGCTAAGTTCACTTAATATGGACAACTCTTTTTAAACTATTTTGATGCATGGTCTTGCCATAAAGTTCAATATGAAAACGTTGGTTGGCTCTTATTTCTATGTTTTTAATTCTTCTTTTTATTCCTAGTGTGAGGCATGTGGGTGGTTGTTTGTCCTATTTTGTTTGTTGTATCTTAAATATTGCTTTTTAAACGCAGGATGGGATGCTTAAGCATAAAGGTACATATGAAATCATGGCCCCTGAAGATATTGGCTATGAACGATCCAATGATGCTGGAATTGTTTTAGGAAAACTTAGGTATGCCATGTTTTTTTTTTCTTTTTGAAACGGAGACAAGCTTTTTTATTAATGTCATGTTGTCTTATGGAAAATGCCTTATAGAAAATGACAATGTTGCCTTAAGGAAACTTTGATATGCCATGCACACCATTTGAAAATTAGAATACGTATGAAATATATAATATTGAAACCTAGGTAGGATTTCCATAAGTGGTCGATTAAACGTACTTTGAAGCAACTTTCTTCCTTTTATGAACAAAAAGAGAAAAATGGATTGACCTAGTAGTAAAAAAGGGCCAGGAGTGTAATGGATTCAATTTATAGTAACCACCTACTTTGGTTTTAATATCCTACTGATTAGACCTTTCCTCATGGGAGACAAACTTCTTAATTGGCTCCTTGTAGCTAATTATGTCTTTTGAAGATTGGTATTTTCACTCTTTACTTGTAGCATGCACGTGCATTTCTTTTTTGTTCTCTCTCTAGTTTTCTGAGTGTATGTACTTGGTTTTGTACCTGATATGTTTCAACATCTGTAAAACTCTTAGGTTTGACTTTTGAGAGAATCTGAATTATTTACTCCCTTCACTAAAGAGATGTTTATATACAAGGTACAAGAATGACCTAAGTAGGAAAGCATAAAAGGACCATAAAAGGACAATGTAATAAAGATATTTACAGTAATAAAATAACTCAATATTAGTACCCCAACACCTGTGATGGATTTAATAATTAAAGTTTTACCAAATTCTTATCAGCACTATCTCTCAACGTGCCTAGTGTATTTCAGTGACATTTCAAGTGTCTGATGTGTAAATTTGTGACACTTCTCCAGAATAGAGTTTATGTGATTTGTGGAGGCCAGGTACAGTAAGTACCTCCACTAAAAGGACTAGAATCTACTAGGCAGGACTCAGGAGAACCTGATAAAAAGAAAAATCTGGCAAGAAGCCAGCAGGGGGAAAACTTCCTATTCTATTCATTCAATTTCAAAATCGTACCATATAAAATAATAATAATAAAACAAAAAACAAAACAAAACAAAGAAGTCCAAAATCATACCACTCCTGCCAGAGCTCTCTCTGAAAGTCTAAGGGCTACAGACTACCCTTTCCTTCTTTCTTAGTGGATTAGTTTTTTTGCATCAAGTGGAACTTCTGTTGAGTTACCTTGTTCTCTCCTCTTCTTTGCTGCTCCCGAATATAATCCTTTTCTTGTCTCTTTGAAACCCTTGATATCTTCCCATTTTCTTTCTACAATAAATTAATCATCTAATCATCTAATATTTATCAAAAGAAAAAAAAATAGTTTAATCATCTAATAAAGTAACTTGTGGTATTTTGTTTCTTGCCGATTTCATGCATGTTCGTAGAATGTTAATTTGTGATATTTAACAATTTTCCTTGTATTGCCGTGGTCCGAAGGGACATGTTACTTTTATTCTTTTCATTATATTGGGCCTATAGACATACTGTTTTTTTAAGAGAAACAATTGCATTTGGTTTAAAAAGGAAGCATATGAGTAATAAACAAAAAGATTTGAACTGTTACACCAGGAAATACCATAATAAACTGCAAAAAGATGCTCTTTATCCATGAAGACTAGTTTCTCTTTTTCCAAAGAACCCAAAAGAAGGCGCAAGATATATCTACGAAAGAGCTGTAGCATACTTGAAAGAATGACCCATGAAATAGATTTAAGGTGGGCAGTGACATCTCTAGTGAAAACCAAATGCATTCAGAAGCTCTGCATGTTTTATTCCAAAGTTACTGTTTAAGATCTTTGTGCACCAATAGAGGTTGTACACCTTTTGAAGCACCGGAAACCCCTCTTTGTTGAGATTCTTTTACTGTTCCTAGAGGATCTTAGTACCGTTGGACACTGTAATAAATGTAACTGCTGAAGGAGACTGGAGAGGATATTGAAGAAGCTATCTGGTCAGAAAATGCATAATTTGTGAGGCATTTTCTGTTCCAAGGTCAGACTAAGTTTTAAAATATTTAAATAGAAGTTAACATTGCGTCTTCTTTTATCCATAAGTCATCCTATCGGGTTCGATGATCACCTGTACCAAGGGTGGTAAAGTAGTTTTTTCTGGGACAGGAGAAATGTGAAACATACGCTGCGGTTCTATTGTAGTGTTAGCCAATTAATTTTTTTTTTCTTTTACAACATTACCAGGATTCACTTTCTGTGTTTAAGTATTTCGCTTCTTGTATTTTGTTCAAAATATACTCCCCAGTAACATGGTTCATGACTACTTTTACAGTGGACGGCATGCCCTTAAAAGTCTCCTTCTAGAGGTAAATTTGGATCTTGAAGCTCAATATTTTTTAGGCAATCCTTCCATATTTTGTCATCCTTTCTGGAAATAGTCTGATGTTATTTTCCGTCTTTTCAGCTTGGTTATGAACTTGATGGTGAGAATCTCGACAATGTATTCTGGCGTTTCAAAGCAGTGGCTGAACAAAAAAAGGTAACTCAAAATTTTGTCCCTCTATTTTGCTGAGCTATTCCCTTCCCTTCTTCCTGCATATAGTGGGATTCTACTTCAACCCGTTTCGAGAACATAAATAACTACTCAAATATAAATTTCTTCATATAATTTCATGCCTTCATTCCTAATTGTCAAATTCTGTTTTGATTTTCCTTGCCCTACTTTATCTTTTACTTTTCAGAGAGTTACAGATGCGGATCTTCGAGCTTTGGTATCCGATGAAGTTTTTCAACCGACTGTTCTTTGGAAGCTGCTAGATATGCAGGTTATTTAAATAACTATAAACCGTCAGATTCAGAAAGTGGTTTGGAGCTATTTTTTTATACATGTATGCATCATTTGACGACACCTTAAATGCTAGGTTACTTGTGGAACTCTTGGTCTTTCCACTGCTACAGTTAAACTCCTTGATGCTGATGGGAAAGAGCACATTGCTTGTTCTGTTGGAACGGGTCCTGTAGATTCAGCTTACAAGGCTGTGGATCTCATTGTTAAGGTTTGTGAAACTTTACATACATCTTCAGATTGGTTTCCTAGTGAATTAAAACTAGGATATTTCAGATATTTAGTTTCAAGCTTACAACAAAGTATATGACCGGGAAATTTAGGAAATTAGTTATTTGGATAATATGCATTAAATTTTGATCCAAAACATAATGGCCCATTCGTTTTCTCCTGAATAGTTTCAGATGTCAGCTTGTTGTGTAGCCATGAGATTTGGTGGATATAATAACATAAAAAGTCGTCATGTCAATAGCCAGGCATTCATGCCTTTAATACTAGTTCTGTGCTATTCAGGTGTTGTTTGCCATATACAGACACACACACATACGTATATTTTGACTTGAAACAGAAGTTTTTCTTTTCTTTTGTCATATATTCTTTGCTGATTGAGTGTGTCATTCCTGAGTGAATGTGACAGTGTAGAGTTGTCATTTTCCTGGATGCAGGAACCAGCAACTCTTCTCGAGTATTCAATGAATGCCGTCACTGAAGGTATAGATGCAATTGCCACGACTCGTGTCCTAATAAGAGGGGACAAAAGCTACACATCTACTAATGCATTGACTGGAGAAGCAGTTCAACGAACATTTAGGTAACAAAAGTTATCCACAAAATATCGTGATACATGCATATTTTGAAGGAAATATATATGTGAAGTTGTCAGCTTTTTTTTCTTTAGGTGAAGTCCTCATATTCACTTTTAAGCACTCATTTAGTATACATGATTGGCTCGTTAAGGCAAAGTTTCGAAGGTCAGGATTGACCTAAAAAAAGCATGCTTTAAAATTTACATTAGCCCACCTTAACAATTATTAAGGTAAAAAGTGTAATTAACATTGGGAACTAAACTTATATGGCCTATTTTAAAAAGACCATATAGCAAGAGGAGAGGCCGCCTCTGCCCCCTGAAAGTATCCAAGGCGCTCACCTTAGTGCGCCACTTTTTTGCATCATCGCCATAAAGTCAGGTGATCACATATTACATTGGCAAAAGAATCCCATTAATATTAAAAATCAAAGAGTTTGAAGAAAGATAATAATACAAAGACGAAAAGCAAGAAGCTTAGAATAAGAAGAATAAAAAGAAGATAAGAACGAAGAAATAAGAAGGGAAAAAAGAGAGAACGAATTACGAAGAACTAAAGATGGTGGGCGGAAGATCAAAATAAGAAACCGAACCTTTAAAAAAAGAGAAAGTCAGAAACATTAAAGCTGCATGTTGCATGCTGTTATGATAGATGTCCATCTCTTTTTCCATTAAATTTAATGGACACCATTGTTTTCTTCCATGACTAACAAATGGCATTTTAACCGTTTTTCTACTATTTTTAAGAAGGAAAAAGAAAAAACATTCTTTACTTTATTTGCCTGAATATCTTTACTGCCAAACTTTATTCAAATATTGAATAGTTTTCTTCCTATTTTTCTTGTTTTAAAAATACAATTTATTATTTTTATCTATAAATAGTTATATATAATATTTATTTAGCATTTTATTACAAGTGCAGCACATGCACTGAAGTGACACCTTTTATCTCCTCTTGTCTGAAGGCGATTGGGAGACTCATGTTCTTAATGTGCGTCTTGCCCTTTAAAAACACTAAATAGAGTAATCTTCAAAGTAATCTTGTTCGAAGTATTATTGCTTCTATGTTCTTGGACTAAATCATATTCAACTTGAAAAGATCCTGTTGACCTAGTTATTAAAGAGATTTACAAGGAATGAGAATTTAAACAAGGTAGCAAGCTTGTTGAGAACCGAATGTTGTAGATGGAATATTAATCAAGTTGTATCTAGGGAAGGGTTCAAACTATACAATGGTTATCGACTTTGGATTTCAGATCTCAAATTTCTTCTGCATCATATGAGGTATAGGTGGTTGGCTTGTAAGATTAGACAAAATAGAGACTATAAGTTGATTCAAACACTCACCAATTATCAAGTTCTGTAGTGAAATCTATAGGTGTTCGATGGTCTTCTCTGGGTGCAGTAGTTGCACAACTACGGTATGTCGATTAACTGTGTATCTACGTTTTTATCATCGCAGTGGTATTGGAGCAGGAATGGACATAGTTGTATCAAGTGTTAAGGCCTACATTGGTGCTCTAAATAAGATGCTTGGTTTTCAAGGAATTGACATTAAGGTGACTGAAGAAAAAACATTATCGGCTTAAAAATCTGATTGATTCTTGAAGGAAGGATGATGGTGGCAGTCTTTCATAAAAGGATTCCATTATTCTGCTTTAAGTGTTATTATCTAATTGTTGTTTCTAATGTGTTTTGTGTTATTTGAACTCCTTTTTTTTTTTGGTCAGTTGGCAAAACTTGTTTCCTTAGCCAGTTATGTGTGTTAATTAGTGTTGTAGTTTTTACGCAGTGAACTGGCTTTTTTAGTAAAATAATGTTAATTATTATTATAATAAAAGGTTTTATCAGTCATTTCAT

mRNA sequence

AAAAAAGAAAAAGAATAAATGTTTTATTCACCGGTTCCGTTCCGGGTTGAAAGCCCAAAAATCAACTGAAAAACCCATTTTTTGGAAAAATATCAAATGACTTTCTGTCACGGACACGTGCAGTTACGAATTTACCCCTTTTGTTTTTTCCTTTTCACTTCCCTGCTTCTTCTCCGACGAGTGTTCAATATGCGACAGTTGAAGTCTCAGGGTCCCCTCACCGGCATTATATATTATTAGTATGACCTAAACCTCAAGCCACTCACAAACGTCCACTGTAGCTCGCCTCTAAGTAAGTCTTCCCTGTTCTTTATTCATTCCAGATTAATTCCTTCCTCACTTCCTCTCTCACCGTTACCCCTCTGTACAATACAAGTCCATTTCTGTTTTCTTTGATTTCCTTTGTTCTCTTTACCATGGCCCTTCCGGCAGCATTGTCCAGACCCAATCATTCTCTTGCTCGCCAATCCGATTCTCTGTTTCCTGCTCCTCATCGGATTTATAGCAGGAATTTGCAGGTTCTTAAGAATCCTTCACGGTCGTTGTCTTTTAAAACGGCTGCTGCTTCGGCGCCTCATAATGTATCTCCTGTTTACTCTTGTCTTACGGAAAGTATTGTTTCGAATTCTGTACCGAAATCATCAATTAGACGGCCGCCGTATATTCCTAATCGCATCCCTGACCCTTCCTATGTTCGCGTATTCGATACCACTCTCCGTGATGGCGAGCAGTCTCCAGGTGCTTCCCTCACTGTTAAAGAGAAGCTTGACATTGCTCGTCAGCTTGCAAAGCTTGGTGTAGATATCATTGAGGCTGGATTCCCTGCTGCTTCGAAGGAAGACTTTGAGGCAGTCAAGATGATTGCAAAAGAGATTGGAAATGCTGTCGATGAGGATGGATATGTGCCTGTTATTTGTGGTCTCTCCAGGTGTAATGAGAAGGATATTCGAACTGCATGGGAGGCTGTCAAATACGCTAAAAGACCGCGGATACATACCTTTATTGCAACGAGCGAAATTCATATGGAGCATAAGCTGAGAAAAACGAAGGAGGAAGTGATTGAAATTGCTCGAAACATGGTGCGGTTTGCACGAAGTTTGGGTTGTGATGATGTTGAGTTCAGCCCCGAGGATGCTGGCAGATCTGACAGAGAGTTCCTTTATCAAATTCTGGGGGAAGTGATAAAGGCTGGGGCAACTACTCTCAACATACCTGATACTGTTGGTTATACAATGCCTTTTGAATTTGGGAAGTTGATTGCTGATATAAAATCTAATACTCCTGGCATTGAAAATGTCATCATTTCATCACACTGCCAGAATGATCTTGGACTAGCAACTGCTAACACAGTAGCAGGGGCATGTGCGGGTGCAAGGCAAGTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCTTCCCTAGAGGAAGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATACTGGAATTAACTCAAGACATATCTTTTTGACTAGCAAGATGGTAGAAGAGTTCACTGGTTTGAATGTACAACCACACAAGGCTATTGTCGGAGCTAATGCTTTTGCTCATGCAAGTGGCATCCATCAGGATGGGATGCTTAAGCATAAAGGTACATATGAAATCATGGCCCCTGAAGATATTGGCTATGAACGATCCAATGATGCTGGAATTGTTTTAGGAAAACTTAGTGGACGGCATGCCCTTAAAAGTCTCCTTCTAGAGCTTGGTTATGAACTTGATGGTGAGAATCTCGACAATGTATTCTGGCGTTTCAAAGCAGTGGCTGAACAAAAAAAGAGAGTTACAGATGCGGATCTTCGAGCTTTGGTATCCGATGAAGTTTTTCAACCGACTGTTCTTTGGAAGCTGCTAGATATGCAGGTTACTTGTGGAACTCTTGGTCTTTCCACTGCTACAGTTAAACTCCTTGATGCTGATGGGAAAGAGCACATTGCTTGTTCTGTTGGAACGGGTCCTGTAGATTCAGCTTACAAGGCTGTGGATCTCATTGTTAAGGAACCAGCAACTCTTCTCGAGTATTCAATGAATGCCGTCACTGAAGGTATAGATGCAATTGCCACGACTCGTGTCCTAATAAGAGGGGACAAAAGCTACACATCTACTAATGCATTGACTGGAGAAGCAGTTCAACGAACATTTAGTGGTATTGGAGCAGGAATGGACATAGTTGTATCAAGTGTTAAGGCCTACATTGGTGCTCTAAATAAGATGCTTGGTTTTCAAGGAATTGACATTAAGGTGACTGAAGAAAAAACATTATCGGCTTAAAAATCTGATTGATTCTTGAAGGAAGGATGATGGTGGCAGTCTTTCATAAAAGGATTCCATTATTCTGCTTTAAGTGTTATTATCTAATTGTTGTTTCTAATGTGTTTTGTGTTATTTGAACTCCTTTTTTTTTTTGGTCAGTTGGCAAAACTTGTTTCCTTAGCCAGTTATGTGTGTTAATTAGTGTTGTAGTTTTTACGCAGTGAACTGGCTTTTTTAGTAAAATAATGTTAATTATTATTATAATAAAAGGTTTTATCAGTCATTTCAT

Coding sequence (CDS)

ATGGCCCTTCCGGCAGCATTGTCCAGACCCAATCATTCTCTTGCTCGCCAATCCGATTCTCTGTTTCCTGCTCCTCATCGGATTTATAGCAGGAATTTGCAGGTTCTTAAGAATCCTTCACGGTCGTTGTCTTTTAAAACGGCTGCTGCTTCGGCGCCTCATAATGTATCTCCTGTTTACTCTTGTCTTACGGAAAGTATTGTTTCGAATTCTGTACCGAAATCATCAATTAGACGGCCGCCGTATATTCCTAATCGCATCCCTGACCCTTCCTATGTTCGCGTATTCGATACCACTCTCCGTGATGGCGAGCAGTCTCCAGGTGCTTCCCTCACTGTTAAAGAGAAGCTTGACATTGCTCGTCAGCTTGCAAAGCTTGGTGTAGATATCATTGAGGCTGGATTCCCTGCTGCTTCGAAGGAAGACTTTGAGGCAGTCAAGATGATTGCAAAAGAGATTGGAAATGCTGTCGATGAGGATGGATATGTGCCTGTTATTTGTGGTCTCTCCAGGTGTAATGAGAAGGATATTCGAACTGCATGGGAGGCTGTCAAATACGCTAAAAGACCGCGGATACATACCTTTATTGCAACGAGCGAAATTCATATGGAGCATAAGCTGAGAAAAACGAAGGAGGAAGTGATTGAAATTGCTCGAAACATGGTGCGGTTTGCACGAAGTTTGGGTTGTGATGATGTTGAGTTCAGCCCCGAGGATGCTGGCAGATCTGACAGAGAGTTCCTTTATCAAATTCTGGGGGAAGTGATAAAGGCTGGGGCAACTACTCTCAACATACCTGATACTGTTGGTTATACAATGCCTTTTGAATTTGGGAAGTTGATTGCTGATATAAAATCTAATACTCCTGGCATTGAAAATGTCATCATTTCATCACACTGCCAGAATGATCTTGGACTAGCAACTGCTAACACAGTAGCAGGGGCATGTGCGGGTGCAAGGCAAGTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCTTCCCTAGAGGAAGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCATACTGGAATTAACTCAAGACATATCTTTTTGACTAGCAAGATGGTAGAAGAGTTCACTGGTTTGAATGTACAACCACACAAGGCTATTGTCGGAGCTAATGCTTTTGCTCATGCAAGTGGCATCCATCAGGATGGGATGCTTAAGCATAAAGGTACATATGAAATCATGGCCCCTGAAGATATTGGCTATGAACGATCCAATGATGCTGGAATTGTTTTAGGAAAACTTAGTGGACGGCATGCCCTTAAAAGTCTCCTTCTAGAGCTTGGTTATGAACTTGATGGTGAGAATCTCGACAATGTATTCTGGCGTTTCAAAGCAGTGGCTGAACAAAAAAAGAGAGTTACAGATGCGGATCTTCGAGCTTTGGTATCCGATGAAGTTTTTCAACCGACTGTTCTTTGGAAGCTGCTAGATATGCAGGTTACTTGTGGAACTCTTGGTCTTTCCACTGCTACAGTTAAACTCCTTGATGCTGATGGGAAAGAGCACATTGCTTGTTCTGTTGGAACGGGTCCTGTAGATTCAGCTTACAAGGCTGTGGATCTCATTGTTAAGGAACCAGCAACTCTTCTCGAGTATTCAATGAATGCCGTCACTGAAGGTATAGATGCAATTGCCACGACTCGTGTCCTAATAAGAGGGGACAAAAGCTACACATCTACTAATGCATTGACTGGAGAAGCAGTTCAACGAACATTTAGTGGTATTGGAGCAGGAATGGACATAGTTGTATCAAGTGTTAAGGCCTACATTGGTGCTCTAAATAAGATGCTTGGTTTTCAAGGAATTGACATTAAGGTGACTGAAGAAAAAACATTATCGGCTTAA

Protein sequence

MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVYSCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA*

Homology

[Close]

BLAST of CSPI03G22250 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 4.9e-271
Identity = 468/579 (80.83%), Postives = 525/579 (90.67%), Query Frame = 0


Query: 41  RSLSFKTAAASAPHNVSPVYSCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTL 100
           RS + +T + +        YS    S +    P+   RRP YIPNRI DP+YVR+FDTTL
Sbjct: 38  RSQNHRTISLTTAGKFRVSYSLSASSPLPPHAPR---RRPNYIPNRISDPNYVRIFDTTL 97

Query: 101 RDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDED 160
           RDGEQSPGA+LT KEKLDIARQLAKLGVDIIEAGFPAASK+DFEAVK IA+ +GN VDE+
Sbjct: 98  RDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDEN 157

Query: 161 GYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARN 220
           GYVPVICGLSRCN+KDI TAWEAVKYAKRPRIHTFIATS+IH+++KL+K+KEEVIEIARN
Sbjct: 158 GYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARN 217

Query: 221 MVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKL 280
           MVRFARSLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKAGATTLNIPDTVG T+P EFG+L
Sbjct: 218 MVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQL 277

Query: 281 IADIKSNTPGIENVIISSHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE 340
           IADIK+NTPGI+NVIIS+HCQNDLGL+TANT++GA +GARQVEVTINGIGERAGNASLEE
Sbjct: 278 IADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEE 337

Query: 341 VVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQ 400
           VVMA++CRG+HVLGGL TGI++RHI +TSKMVEE+TG+  QPHKAIVGANAFAH SGIHQ
Sbjct: 338 VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQ 397

Query: 401 DGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFW 460
           DGMLKHKGTYEIM+PE+IG ERSNDAGIVLGKLSGRHALK  L ELGY LD   L N+FW
Sbjct: 398 DGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFW 457

Query: 461 RFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKE 520
           RFKAVAEQKKRVTDADL ALVSDEVFQP  +WKLLDMQ+TCGTLGLST+TVKL D+DGKE
Sbjct: 458 RFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKE 517

Query: 521 HIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTN 580
           H+ACSVGTGPVD+AYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGD +Y+STN
Sbjct: 518 HVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTN 577

Query: 581 ALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 620
           A+TGE+V+RTFSG GAGMDIVVSSVKAY+GALNKMLGF+
Sbjct: 578 AVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGFK 613

[Close]

BLAST of CSPI03G22250 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 922.5 bits (2383), Expect = 2.5e-267
Identity = 453/541 (83.73%), Postives = 503/541 (92.98%), Query Frame = 0


Query: 79  RPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGFPAA 138
           RP YIPNRI DP+YVRVFDTTLRDGEQSPGA+LT KEKLDIARQLAKLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 139 SKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIAT 198
           SK+DFEAVK IA+ +GN VDE+GYVPVICGLSRCN+KDI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 199 SEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKA 258
           S+IH+E+KL+KTK EVIEIAR+MVRFARSLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 259 GATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHCQNDLGLATANTVAGACAG 318
           GATTLNIPDTVG T+P EFG+LI D+K+NTPGIENV+IS+HCQNDLGL+TANT++GA AG
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 319 ARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGL 378
           ARQ+EVTINGIGERAGNASLEEVVMA++CRG+HVLGGL TGI++RHI +TSKMVEE+TG+
Sbjct: 315 ARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGM 374

Query: 379 NVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHA 438
             QPHKAIVGANAFAH SGIHQDGMLKHKGTYEI+ PE+IG ERSNDAGIVLGKLSGRHA
Sbjct: 375 QTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHA 434

Query: 439 LKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQ 498
           LK  L ELGY+LD E L  +FWRFK VAEQKKRVTDAD+ ALVSDEVFQP  +WKLLD+Q
Sbjct: 435 LKDRLTELGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQ 494

Query: 499 VTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTE 558
           +TCGTLGLSTATVKL DADGKEH+ACS+GTGPVDSAYKAVDLIVKEPATLLEYSMNAVTE
Sbjct: 495 ITCGTLGLSTATVKLADADGKEHVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTE 554

Query: 559 GIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 618
           GIDAIATTRVLIRG   Y+STNA+TGE VQRTFSG GAGMDIVVSSVKAY+GALNKM+ F
Sbjct: 555 GIDAIATTRVLIRGSNKYSSTNAITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDF 614

Query: 619 Q 620
           +
Sbjct: 615 K 615

[Close]

BLAST of CSPI03G22250 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 6.9e-249
Identity = 418/544 (76.84%), Postives = 492/544 (90.44%), Query Frame = 0


Query: 76  SIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGF 135
           S RRP Y+P++I DP YVR+FDTTLRDGEQSPGA++T KEKLD+ARQLAKLGVDIIEAGF
Sbjct: 31  SNRRPEYVPSKISDPKYVRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIEAGF 90

Query: 136 PAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTF 195
           PA+S+ DFE+VK+IA+EIGN  DE+G+VPVICGLSRCN+ DI  AWEAVKYAK+PR+HTF
Sbjct: 91  PASSEADFESVKLIAEEIGNNTDENGFVPVICGLSRCNKSDIDKAWEAVKYAKKPRVHTF 150

Query: 196 IATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEV 255
           IATSEIHM++KL+ ++E+V+E AR+MV +ARSLGC+DVEFSPEDAGRSDREFLY ILGEV
Sbjct: 151 IATSEIHMKYKLKMSREQVVEKARSMVAYARSLGCEDVEFSPEDAGRSDREFLYDILGEV 210

Query: 256 IKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHCQNDLGLATANTVAGA 315
           IKAGATTLNIPDTVGYT+P EFG+LI DIK+NTPGIENVIIS+HCQNDLGL+TANT+AGA
Sbjct: 211 IKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQNDLGLSTANTLAGA 270

Query: 316 CAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEF 375
           CAGARQ+EVTINGIGERAGNASLEEVVMAL+CRGE VLGGL+TGIN++HI  +SKMVEE+
Sbjct: 271 CAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHIVPSSKMVEEY 330

Query: 376 TGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSG 435
           +GL VQPHKAIVGANAFAH SGIHQDGMLKHK TYEI++P+D+G  RSN+AGIVLGKLSG
Sbjct: 331 SGLQVQPHKAIVGANAFAHESGIHQDGMLKHKDTYEIISPDDVGLSRSNEAGIVLGKLSG 390

Query: 436 RHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLL 495
           RHALKS +LELGY++DG+ L+++FWRFK+VAE+KK++TD DL AL+SDEV QP V WKL 
Sbjct: 391 RHALKSKMLELGYDIDGKELEDLFWRFKSVAEKKKKITDDDLIALMSDEVLQPNVYWKLG 450

Query: 496 DMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNA 555
           D+Q+ CG+LGLSTATVKL++ DG+EHIACSVGTGPVD+AYKAVDLIVK P TLLEYSMNA
Sbjct: 451 DVQIMCGSLGLSTATVKLINTDGQEHIACSVGTGPVDAAYKAVDLIVKVPITLLEYSMNA 510

Query: 556 VTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKM 615
           VTEGIDAIA+TRV I     +T  N  TG+ + RTFSG GA MD+V+SSV+AYIGALNKM
Sbjct: 511 VTEGIDAIASTRVSICSIDRHTIMNGSTGQTIHRTFSGTGADMDVVISSVRAYIGALNKM 570

Query: 616 LGFQ 620
           L ++
Sbjct: 571 LSYE 574

[Close]

BLAST of CSPI03G22250 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 2.2e-247
Identity = 422/571 (73.91%), Postives = 503/571 (88.09%), Query Frame = 0


Query: 55  NVSPVYSCLTESIVSNSVPKSSIRR-PPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTV 114
           N  P  S  + S  S  V + SIRR P Y P+ IPDP+YVR+FDTTLRDGEQSPGA++T 
Sbjct: 32  NFKPSISKHSNSSFSIPVVRCSIRRIPEYTPSHIPDPNYVRIFDTTLRDGEQSPGATMTT 91

Query: 115 KEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCN 174
           KEKLD+ARQ AKLGVDIIEAGFPA+S+ D EAVK+IAKE+GN V E+ YVPVICGL+RCN
Sbjct: 92  KEKLDVARQSAKLGVDIIEAGFPASSEADLEAVKLIAKEVGNGVYEEEYVPVICGLARCN 151

Query: 175 EKDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDV 234
           +KDI  AWEAVKYAK+PRIHTFIATSE+HM +KL+ ++++V+E AR+MV +ARS+GC+DV
Sbjct: 152 KKDIDKAWEAVKYAKKPRIHTFIATSEVHMNYKLKMSRDQVVEKARSMVAYARSIGCEDV 211

Query: 235 EFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIEN 294
           EFSPEDAGRSD EFLY ILGEVIKAGATTLNIPDTVGYT+P EFG+LIA IK+NTPG+E+
Sbjct: 212 EFSPEDAGRSDPEFLYHILGEVIKAGATTLNIPDTVGYTVPEEFGQLIAKIKANTPGVED 271

Query: 295 VIISSHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVL 354
           VIIS+HCQNDLGL+TANT+AGACAGARQ+EVTINGIGERAGNASLEEVVMAL+CRGE VL
Sbjct: 272 VIISTHCQNDLGLSTANTLAGACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVL 331

Query: 355 GGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIM 414
           GGL+TGIN++HI ++SKMVE  +GL+VQPHKAIVGANAF H SGIHQDGMLKHK TYEI+
Sbjct: 332 GGLYTGINTQHILMSSKMVEGISGLHVQPHKAIVGANAFVHESGIHQDGMLKHKDTYEII 391

Query: 415 APEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVT 474
           +PEDIG  R+N++GIV GKLSG    K  +LELGYE++G+ LD++FWRFK+VAE+KK++T
Sbjct: 392 SPEDIGLNRANESGIVFGKLSGVMLCKPKMLELGYEIEGKELDDLFWRFKSVAEKKKKIT 451

Query: 475 DADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDS 534
           D DL AL+SDEVFQP  +W+L ++QVTCG+LGLSTATVKL+DADG+EHI+CSVGTGPVD+
Sbjct: 452 DDDLVALMSDEVFQPQFVWQLQNVQVTCGSLGLSTATVKLIDADGREHISCSVGTGPVDA 511

Query: 535 AYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSG 594
           AYKAVDLIVK P TLLEYSMNAVT+GIDAIA+TRVLIRG+  +TST+ALTGE V RTFSG
Sbjct: 512 AYKAVDLIVKVPVTLLEYSMNAVTQGIDAIASTRVLIRGENGHTSTHALTGETVHRTFSG 571

Query: 595 IGAGMDIVVSSVKAYIGALNKMLGFQGIDIK 625
            GA MDIV+SSV+AY+GALNKM+ F+ +  K
Sbjct: 572 TGADMDIVISSVRAYVGALNKMMSFRKLMAK 602

[Close]

BLAST of CSPI03G22250 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 5.1e-220
Identity = 383/551 (69.51%), Postives = 455/551 (82.58%), Query Frame = 0


Query: 66  SIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAK 125
           S  S+  PK S  RP YIPN IPD SYVR+ DTTLRDGEQSPGA++T KEKLDIARQL K
Sbjct: 6   STPSSQSPKLSHLRPQYIPNHIPDSSYVRILDTTLRDGEQSPGATMTAKEKLDIARQLVK 65

Query: 126 LGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVK 185
           LGVDII+ GFP+AS  DF AVKMIA+E+GNAVD+DGYVPVI G  RC EKDI TAWEAVK
Sbjct: 66  LGVDIIQPGFPSASNSDFMAVKMIAQEVGNAVDDDGYVPVIAGFCRCVEKDISTAWEAVK 125

Query: 186 YAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDAGRSDR 245
           YAKRPR+ T IATS IHMEHKLRK+K++VI+IAR+MV+FARSLGC+D++F  EDA RSDR
Sbjct: 126 YAKRPRLCTSIATSPIHMEHKLRKSKDQVIQIARDMVKFARSLGCNDIQFGAEDATRSDR 185

Query: 246 EFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHCQNDLG 305
           EFLY+ILG VI+AGATT+NI DTVG  MP E GKLI DIK NTPGI NVIIS+HC NDLG
Sbjct: 186 EFLYEILGVVIEAGATTVNIADTVGIVMPLELGKLIVDIKDNTPGIANVIISTHCHNDLG 245

Query: 306 LATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRHI 365
           LATANT+ GA  GARQ+EVTINGIGERAGNASLEEVVMAL  +G+H L GL+T IN+RHI
Sbjct: 246 LATANTIEGARTGARQLEVTINGIGERAGNASLEEVVMALASKGDHALNGLYTRINTRHI 305

Query: 366 FLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGYERSND 425
             TSKMVEE++G+++QPHK +VGANAF HASGIHQDGMLKHKGTYE ++PE+IG++R+  
Sbjct: 306 LETSKMVEEYSGMHLQPHKPLVGANAFVHASGIHQDGMLKHKGTYETISPEEIGHKRTTR 365

Query: 426 AGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRALVSDEV 485
            GIVLGKLSG  AL+  L ELGY+L  + +D+VFW+FKA+AE+KK VTD DL+ALVS + 
Sbjct: 366 IGIVLGKLSGSQALRKRLEELGYDLKEDEVDSVFWQFKAMAEKKKVVTDVDLKALVSYKA 425

Query: 486 FQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDLIVKEP 545
           F    +WKL D+QVTCGT+GLSTATVKL++ DG  H+ACS+G G VDS YKA++LIVKEP
Sbjct: 426 FHAESIWKLGDLQVTCGTIGLSTATVKLVNIDGSTHVACSIGIGAVDSTYKAINLIVKEP 485

Query: 546 ATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDIVVSSV 605
             LL+YS+N+VTEGI    T RV+I  + ++TST A T +A   TFSGI A MD+VVS+V
Sbjct: 486 TKLLDYSLNSVTEGIGVNVTARVVICRENNHTSTYAFTEDANYPTFSGIAAEMDVVVSTV 545

Query: 606 KAYIGALNKML 617
           KAY+ ALNK+L
Sbjct: 546 KAYLVALNKLL 556

[Close]

BLAST of CSPI03G22250 vs. ExPASy TrEMBL
Match: A0A0A0L7C8 (2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1)

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 632/633 (99.84%), Postives = 633/633 (100.00%), Query Frame = 0


Query: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60
           MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY
Sbjct: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60

Query: 61  SCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120
           SCLTESIVSNSVPK+SIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA
Sbjct: 61  SCLTESIVSNSVPKASIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300
           GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300

Query: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360
           QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360

Query: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420
           NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY
Sbjct: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420

Query: 421 ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL 480
           ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL
Sbjct: 421 ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL 480

Query: 481 VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540
           VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL
Sbjct: 481 VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540

Query: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600
           IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI
Sbjct: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600

Query: 601 VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 634
           VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA
Sbjct: 601 VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 633

[Close]

BLAST of CSPI03G22250 vs. ExPASy TrEMBL
Match: A0A5D3BP53 (2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00350 PE=3 SV=1)

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 607/633 (95.89%), Postives = 617/633 (97.47%), Query Frame = 0


Query: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60
           MALPAALSRPNH LARQSDSLFPA H IY+RN QVLKNPSRSLSFKTAAA AP NVS +Y
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAA-APDNVSRIY 60

Query: 61  SCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120
            CLTES+VSNS PKSS RRPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEKLDIA
Sbjct: 61  CCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIG+AVDEDGYVPVICGLSRCNEKDIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMEHKLRKT+E+VIEIARNMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300
           GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIS+HC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360
           QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360

Query: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420
           N RHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY
Sbjct: 361 NPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420

Query: 421 ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL 480
           ERSNDAGIVLGKLSGRHALKS LLELGYELD ENL NVFWRFKAVAEQKKRVTDADLRAL
Sbjct: 421 ERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFWRFKAVAEQKKRVTDADLRAL 480

Query: 481 VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540
           VSDEVFQPTVLWKLLD+QVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL
Sbjct: 481 VSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540

Query: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600
           IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI
Sbjct: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600

Query: 601 VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 634
           VVSSVKAYIGALNKMLGFQGIDIK T+EKTLSA
Sbjct: 601 VVSSVKAYIGALNKMLGFQGIDIKETKEKTLSA 632

[Close]

BLAST of CSPI03G22250 vs. ExPASy TrEMBL
Match: A0A1S3C4S6 (2-isopropylmalate synthase OS=Cucumis melo OX=3656 GN=LOC103496846 PE=3 SV=1)

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 603/631 (95.56%), Postives = 614/631 (97.31%), Query Frame = 0


Query: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKT--AAASAPHNVSP 60
           MALPAALSRPNH LARQSDSLFPA H IY+RN QVLKNPSRSLSFKT  AAA+AP NVS 
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAAAAAAPDNVSR 60

Query: 61  VYSCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLD 120
           +Y CLTES+VSNS PKSS RRPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEKLD
Sbjct: 61  IYCCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLD 120

Query: 121 IARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIR 180
           IARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIG+AVDEDGYVPVICGLSRCNEKDIR
Sbjct: 121 IARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIR 180

Query: 181 TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPE 240
           TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKT+E+VIEIARNMVRFARSLGCDDVEFSPE
Sbjct: 181 TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPE 240

Query: 241 DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISS 300
           DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIS+
Sbjct: 241 DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIST 300

Query: 301 HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHT 360
           HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHT
Sbjct: 301 HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHT 360

Query: 361 GINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDI 420
           GIN RHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDI
Sbjct: 361 GINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDI 420

Query: 421 GYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLR 480
           GYERSNDAGIVLGKLSGRHALKS LLELGYELD ENL NVFWRFKAVAEQKKRVTDADLR
Sbjct: 421 GYERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFWRFKAVAEQKKRVTDADLR 480

Query: 481 ALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAV 540
           ALVSDEVFQPTVLWKLLD+QVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAV
Sbjct: 481 ALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAV 540

Query: 541 DLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGM 600
           DLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGM
Sbjct: 541 DLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGM 600

Query: 601 DIVVSSVKAYIGALNKMLGFQGIDIKVTEEK 630
           DIVVSSVKAYIGALNKMLGFQGIDIK T+EK
Sbjct: 601 DIVVSSVKAYIGALNKMLGFQGIDIKETKEK 631

[Close]

BLAST of CSPI03G22250 vs. ExPASy TrEMBL
Match: A0A5A7UXZ0 (2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold72G00370 PE=3 SV=1)

HSP 1 Score: 1164.8 bits (3012), Expect = 0.0e+00
Identity = 600/634 (94.64%), Postives = 612/634 (96.53%), Query Frame = 0


Query: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60
           MALPAALSRPNH LARQSDSLFPA H IY+RN QVLKNPSRSLSFKTAAA AP NVS +Y
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAA-APDNVSRIY 60

Query: 61  SCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120
            CLTES+VSNS PKSS RRPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEKLDIA
Sbjct: 61  CCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIG+AVDEDGYVPVICGLSRCNEKDIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMEHKLRKT+E+VIEIARNMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300
           GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIS+HC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360
           QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360

Query: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420
           N RHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY
Sbjct: 361 NPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420

Query: 421 ERSNDAGIVLGKLSGRHALKSLL-LELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRA 480
           ERSNDAGIVLGKL  R+A+ S L   LGYELD ENL NVFWRFKAVAEQKKRVTDADLRA
Sbjct: 421 ERSNDAGIVLGKL--RYAILSYLKCLLGYELDSENLHNVFWRFKAVAEQKKRVTDADLRA 480

Query: 481 LVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVD 540
           LVSDEVFQPTVLWKLLD+QVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVD
Sbjct: 481 LVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVD 540

Query: 541 LIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMD 600
           LIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMD
Sbjct: 541 LIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMD 600

Query: 601 IVVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 634
           IVVSSVKAYIGALNKMLGFQGIDIK T+EKTLSA
Sbjct: 601 IVVSSVKAYIGALNKMLGFQGIDIKETKEKTLSA 631

[Close]

BLAST of CSPI03G22250 vs. ExPASy TrEMBL
Match: A0A6J1F3K1 (2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111441856 PE=3 SV=1)

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 575/633 (90.84%), Postives = 600/633 (94.79%), Query Frame = 0


Query: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60
           MA+PAALSRPN  L RQ +S F A HRIYSRNLQ LKNPSRSLSF+TA      +VS VY
Sbjct: 1   MAMPAALSRPNQFLVRQPNSQFSARHRIYSRNLQALKNPSRSLSFRTAT----DHVSRVY 60

Query: 61  SCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120
            C TE +V NS  KSSIRRP YIPNRIPD SYVR+FDTTLRDGEQSPGASLT KEKLDIA
Sbjct: 61  CCQTERVVLNSESKSSIRRPSYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKE+GNAVD DGYVPVICGLSRCNEKDIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDADGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240
           WEAVKY+KRPRIHTFIATSEIHMEHKLRKT+E+VIEIARNMV+FARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300
           GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIS+HC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360
           QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360

Query: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420
           NSRHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQDGMLKHKGTYEI+APEDIGY
Sbjct: 361 NSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIIAPEDIGY 420

Query: 421 ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL 480
           ERSNDAGIVLGKLSGRHALKS L+ELGYELD E LDNVFWRFKAVAEQKKRVTDAD+RAL
Sbjct: 421 ERSNDAGIVLGKLSGRHALKSRLVELGYELDNEKLDNVFWRFKAVAEQKKRVTDADIRAL 480

Query: 481 VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540
           VSDEVFQP VLWKLLD+QVTCGTLGLSTATVKLLDADG+EHIAC+VGTGPVDSAYKAVDL
Sbjct: 481 VSDEVFQPIVLWKLLDLQVTCGTLGLSTATVKLLDADGEEHIACAVGTGPVDSAYKAVDL 540

Query: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600
           IVKEPATLLEYSM AVTEGIDAIATTRVLIRG+KSYT+T+A TGE+VQRTFSGIGAGMDI
Sbjct: 541 IVKEPATLLEYSMTAVTEGIDAIATTRVLIRGEKSYTATHASTGESVQRTFSGIGAGMDI 600

Query: 601 VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 634
           VVSSVKAYIGALNKMLGF G+DIKVTE+KTLSA
Sbjct: 601 VVSSVKAYIGALNKMLGFHGVDIKVTEKKTLSA 629

[Close]

BLAST of CSPI03G22250 vs. NCBI nr
Match: XP_011651428.1 (2-isopropylmalate synthase 2, chloroplastic [Cucumis sativus] >KGN57885.1 hypothetical protein Csa_010376 [Cucumis sativus])

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 632/633 (99.84%), Postives = 633/633 (100.00%), Query Frame = 0


Query: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60
           MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY
Sbjct: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60

Query: 61  SCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120
           SCLTESIVSNSVPK+SIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA
Sbjct: 61  SCLTESIVSNSVPKASIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300
           GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300

Query: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360
           QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360

Query: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420
           NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY
Sbjct: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420

Query: 421 ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL 480
           ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL
Sbjct: 421 ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL 480

Query: 481 VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540
           VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL
Sbjct: 481 VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540

Query: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600
           IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI
Sbjct: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600

Query: 601 VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 634
           VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA
Sbjct: 601 VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 633

[Close]

BLAST of CSPI03G22250 vs. NCBI nr
Match: TYK01483.1 (2-isopropylmalate synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 607/633 (95.89%), Postives = 617/633 (97.47%), Query Frame = 0


Query: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60
           MALPAALSRPNH LARQSDSLFPA H IY+RN QVLKNPSRSLSFKTAAA AP NVS +Y
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAA-APDNVSRIY 60

Query: 61  SCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120
            CLTES+VSNS PKSS RRPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEKLDIA
Sbjct: 61  CCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIG+AVDEDGYVPVICGLSRCNEKDIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMEHKLRKT+E+VIEIARNMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300
           GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIS+HC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360
           QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360

Query: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420
           N RHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY
Sbjct: 361 NPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420

Query: 421 ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL 480
           ERSNDAGIVLGKLSGRHALKS LLELGYELD ENL NVFWRFKAVAEQKKRVTDADLRAL
Sbjct: 421 ERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFWRFKAVAEQKKRVTDADLRAL 480

Query: 481 VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540
           VSDEVFQPTVLWKLLD+QVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL
Sbjct: 481 VSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540

Query: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600
           IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI
Sbjct: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600

Query: 601 VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 634
           VVSSVKAYIGALNKMLGFQGIDIK T+EKTLSA
Sbjct: 601 VVSSVKAYIGALNKMLGFQGIDIKETKEKTLSA 632

[Close]

BLAST of CSPI03G22250 vs. NCBI nr
Match: XP_008457079.1 (PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 603/631 (95.56%), Postives = 614/631 (97.31%), Query Frame = 0


Query: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKT--AAASAPHNVSP 60
           MALPAALSRPNH LARQSDSLFPA H IY+RN QVLKNPSRSLSFKT  AAA+AP NVS 
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAAAAAAPDNVSR 60

Query: 61  VYSCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLD 120
           +Y CLTES+VSNS PKSS RRPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEKLD
Sbjct: 61  IYCCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLD 120

Query: 121 IARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIR 180
           IARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIG+AVDEDGYVPVICGLSRCNEKDIR
Sbjct: 121 IARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIR 180

Query: 181 TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPE 240
           TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKT+E+VIEIARNMVRFARSLGCDDVEFSPE
Sbjct: 181 TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPE 240

Query: 241 DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISS 300
           DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIS+
Sbjct: 241 DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIST 300

Query: 301 HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHT 360
           HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHT
Sbjct: 301 HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHT 360

Query: 361 GINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDI 420
           GIN RHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDI
Sbjct: 361 GINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDI 420

Query: 421 GYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLR 480
           GYERSNDAGIVLGKLSGRHALKS LLELGYELD ENL NVFWRFKAVAEQKKRVTDADLR
Sbjct: 421 GYERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFWRFKAVAEQKKRVTDADLR 480

Query: 481 ALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAV 540
           ALVSDEVFQPTVLWKLLD+QVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAV
Sbjct: 481 ALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAV 540

Query: 541 DLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGM 600
           DLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGM
Sbjct: 541 DLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGM 600

Query: 601 DIVVSSVKAYIGALNKMLGFQGIDIKVTEEK 630
           DIVVSSVKAYIGALNKMLGFQGIDIK T+EK
Sbjct: 601 DIVVSSVKAYIGALNKMLGFQGIDIKETKEK 631

[Close]

BLAST of CSPI03G22250 vs. NCBI nr
Match: KAA0060742.1 (2-isopropylmalate synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1164.8 bits (3012), Expect = 0.0e+00
Identity = 600/634 (94.64%), Postives = 612/634 (96.53%), Query Frame = 0


Query: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60
           MALPAALSRPNH LARQSDSLFPA H IY+RN QVLKNPSRSLSFKTAAA AP NVS +Y
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAA-APDNVSRIY 60

Query: 61  SCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120
            CLTES+VSNS PKSS RRPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEKLDIA
Sbjct: 61  CCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLDIA 120

Query: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180
           RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIG+AVDEDGYVPVICGLSRCNEKDIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMEHKLRKT+E+VIEIARNMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300
           GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIS+HC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHC 300

Query: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360
           QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360

Query: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420
           N RHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY
Sbjct: 361 NPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420

Query: 421 ERSNDAGIVLGKLSGRHALKSLL-LELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRA 480
           ERSNDAGIVLGKL  R+A+ S L   LGYELD ENL NVFWRFKAVAEQKKRVTDADLRA
Sbjct: 421 ERSNDAGIVLGKL--RYAILSYLKCLLGYELDSENLHNVFWRFKAVAEQKKRVTDADLRA 480

Query: 481 LVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVD 540
           LVSDEVFQPTVLWKLLD+QVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVD
Sbjct: 481 LVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVD 540

Query: 541 LIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMD 600
           LIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMD
Sbjct: 541 LIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMD 600

Query: 601 IVVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 634
           IVVSSVKAYIGALNKMLGFQGIDIK T+EKTLSA
Sbjct: 601 IVVSSVKAYIGALNKMLGFQGIDIKETKEKTLSA 631

[Close]

BLAST of CSPI03G22250 vs. NCBI nr
Match: XP_038875433.1 (2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 588/634 (92.74%), Postives = 611/634 (96.37%), Query Frame = 0


Query: 1   MALPAALSRPN-HSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPV 60
           MA+PAALSRPN H L+RQS++ FPA HRIYS N QVLKNPSRSLSF+TAA     NVS +
Sbjct: 1   MAMPAALSRPNTHFLSRQSNTQFPARHRIYSTNFQVLKNPSRSLSFRTAA----DNVSRI 60

Query: 61  YSCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDI 120
           Y C TES+VSNS  KSSIRRPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEKLDI
Sbjct: 61  YCCQTESVVSNSGLKSSIRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTAKEKLDI 120

Query: 121 ARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRT 180
           ARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKE+GNAVDEDGYVPVICGLSRCNE DIRT
Sbjct: 121 ARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNENDIRT 180

Query: 181 AWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPED 240
           AWEAVKYAKRPRIHTFIATSEIHMEHKLRKT+E+VIEIARNMVRFARSLGCDDVEFSPED
Sbjct: 181 AWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPED 240

Query: 241 AGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSH 300
           AGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMP EFGKLIADIKSNTPGIENVIIS+H
Sbjct: 241 AGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGKLIADIKSNTPGIENVIISTH 300

Query: 301 CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTG 360
           CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVM+LQCRGEHVLGGLHTG
Sbjct: 301 CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMSLQCRGEHVLGGLHTG 360

Query: 361 INSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIG 420
           INSRHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQDGMLKHKGTYEI++PEDIG
Sbjct: 361 INSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIG 420

Query: 421 YERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRA 480
           YERSN+AGIVLGKLSGRHALKS LLELGYELD ENLDNVFWRFKAVAEQKKRVTDADLRA
Sbjct: 421 YERSNEAGIVLGKLSGRHALKSRLLELGYELDSENLDNVFWRFKAVAEQKKRVTDADLRA 480

Query: 481 LVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVD 540
           LVSDEVFQPTVLWKLLD+QVTCGTLGLSTATVKLLDADGKEHIAC+VGTGPVDSAYKAVD
Sbjct: 481 LVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHIACAVGTGPVDSAYKAVD 540

Query: 541 LIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMD 600
           LIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYT+TNALTGEAVQRTFSGIGAGMD
Sbjct: 541 LIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTATNALTGEAVQRTFSGIGAGMD 600

Query: 601 IVVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 634
           IVVSSVKAYIGALNKMLGF G+DIKVT+EKT+SA
Sbjct: 601 IVVSSVKAYIGALNKMLGFHGVDIKVTQEKTVSA 630

[Close]

BLAST of CSPI03G22250 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 934.9 bits (2415), Expect = 3.5e-272
Identity = 468/579 (80.83%), Postives = 525/579 (90.67%), Query Frame = 0


Query: 41  RSLSFKTAAASAPHNVSPVYSCLTESIVSNSVPKSSIRRPPYIPNRIPDPSYVRVFDTTL 100
           RS + +T + +        YS    S +    P+   RRP YIPNRI DP+YVR+FDTTL
Sbjct: 38  RSQNHRTISLTTAGKFRVSYSLSASSPLPPHAPR---RRPNYIPNRISDPNYVRIFDTTL 97

Query: 101 RDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDED 160
           RDGEQSPGA+LT KEKLDIARQLAKLGVDIIEAGFPAASK+DFEAVK IA+ +GN VDE+
Sbjct: 98  RDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDEN 157

Query: 161 GYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARN 220
           GYVPVICGLSRCN+KDI TAWEAVKYAKRPRIHTFIATS+IH+++KL+K+KEEVIEIARN
Sbjct: 158 GYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARN 217

Query: 221 MVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKL 280
           MVRFARSLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKAGATTLNIPDTVG T+P EFG+L
Sbjct: 218 MVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQL 277

Query: 281 IADIKSNTPGIENVIISSHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEE 340
           IADIK+NTPGI+NVIIS+HCQNDLGL+TANT++GA +GARQVEVTINGIGERAGNASLEE
Sbjct: 278 IADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEE 337

Query: 341 VVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQ 400
           VVMA++CRG+HVLGGL TGI++RHI +TSKMVEE+TG+  QPHKAIVGANAFAH SGIHQ
Sbjct: 338 VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQ 397

Query: 401 DGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFW 460
           DGMLKHKGTYEIM+PE+IG ERSNDAGIVLGKLSGRHALK  L ELGY LD   L N+FW
Sbjct: 398 DGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFW 457

Query: 461 RFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKE 520
           RFKAVAEQKKRVTDADL ALVSDEVFQP  +WKLLDMQ+TCGTLGLST+TVKL D+DGKE
Sbjct: 458 RFKAVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKE 517

Query: 521 HIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTN 580
           H+ACSVGTGPVD+AYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGD +Y+STN
Sbjct: 518 HVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTN 577

Query: 581 ALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFQ 620
           A+TGE+V+RTFSG GAGMDIVVSSVKAY+GALNKMLGF+
Sbjct: 578 AVTGESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGFK 613

[Close]

BLAST of CSPI03G22250 vs. TAIR 10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4 )

HSP 1 Score: 922.5 bits (2383), Expect = 1.8e-268
Identity = 453/541 (83.73%), Postives = 503/541 (92.98%), Query Frame = 0


Query: 79  RPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGFPAA 138
           RP YIPNRI DP+YVRVFDTTLRDGEQSPGA+LT KEKLDIARQLAKLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 139 SKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIAT 198
           SK+DFEAVK IA+ +GN VDE+GYVPVICGLSRCN+KDI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 199 SEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKA 258
           S+IH+E+KL+KTK EVIEIAR+MVRFARSLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 259 GATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHCQNDLGLATANTVAGACAG 318
           GATTLNIPDTVG T+P EFG+LI D+K+NTPGIENV+IS+HCQNDLGL+TANT++GA AG
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 319 ARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGL 378
           ARQ+EVTINGIGERAGNASLEEVVMA++CRG+HVLGGL TGI++RHI +TSKMVEE+TG+
Sbjct: 315 ARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGM 374

Query: 379 NVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHA 438
             QPHKAIVGANAFAH SGIHQDGMLKHKGTYEI+ PE+IG ERSNDAGIVLGKLSGRHA
Sbjct: 375 QTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHA 434

Query: 439 LKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDMQ 498
           LK  L ELGY+LD E L  +FWRFK VAEQKKRVTDAD+ ALVSDEVFQP  +WKLLD+Q
Sbjct: 435 LKDRLTELGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQ 494

Query: 499 VTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTE 558
           +TCGTLGLSTATVKL DADGKEH+ACS+GTGPVDSAYKAVDLIVKEPATLLEYSMNAVTE
Sbjct: 495 ITCGTLGLSTATVKLADADGKEHVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTE 554

Query: 559 GIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 618
           GIDAIATTRVLIRG   Y+STNA+TGE VQRTFSG GAGMDIVVSSVKAY+GALNKM+ F
Sbjct: 555 GIDAIATTRVLIRGSNKYSSTNAITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDF 614

Query: 619 Q 620
           +
Sbjct: 615 K 615

[Close]

BLAST of CSPI03G22250 vs. TAIR 10
Match: AT5G23010.1 (methylthioalkylmalate synthase 1 )

HSP 1 Score: 543.5 bits (1399), Expect = 2.3e-154
Identity = 265/411 (64.48%), Postives = 337/411 (82.00%), Query Frame = 0


Query: 78  RRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGFPA 137
           R P YIPN++PD +YVRVFDTTLRDGEQSPG SLT  +KL+IARQLAKL VDI+E GFP 
Sbjct: 70  RWPEYIPNKLPDGNYVRVFDTTLRDGEQSPGGSLTPPQKLEIARQLAKLRVDIMEVGFPG 129

Query: 138 ASKEDFEAVKMIAKEIGNAVDED-GYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFI 197
           +S+E+ E +K IAK +GN VDE+ GYVPVIC ++RC  +DI   WEA+KYAKRPRI  F 
Sbjct: 130 SSEEELETIKTIAKTVGNEVDEETGYVPVICAIARCKHRDIEATWEALKYAKRPRILVFT 189

Query: 198 ATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVI 257
           +TS+IHM++KL+KT+EEVIE+A + +RFA+SLG +D++F  ED GRSD++FL +ILGE I
Sbjct: 190 STSDIHMKYKLKKTQEEVIEMAVSSIRFAKSLGFNDIQFGCEDGGRSDKDFLCKILGEAI 249

Query: 258 KAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHCQNDLGLATANTVAGAC 317
           KAG T + I DTVG  MP E+G+L+  +K+NTPGI++V+++ HC NDLGLATAN++AG  
Sbjct: 250 KAGVTVVTIGDTVGINMPHEYGELVTYLKANTPGIDDVVVAVHCHNDLGLATANSIAGIR 309

Query: 318 AGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFT 377
           AGARQVEVTINGIGER+GNASLEEVVMAL+CRG +V+ G++T I++R I  TSKMV+E+T
Sbjct: 310 AGARQVEVTINGIGERSGNASLEEVVMALKCRGAYVINGVYTKIDTRQIMATSKMVQEYT 369

Query: 378 GLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGR 437
           GL VQ HK IVGAN F H SGIHQDG+LK++ TYEI++PEDIG  +S ++G+VLGKLSGR
Sbjct: 370 GLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYEILSPEDIGIVKSQNSGLVLGKLSGR 429

Query: 438 HALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRALV--SDEV 486
           HA+K  L ELGYELD E L+ VF  F+ + + KKR+TDADL+ALV  SDE+
Sbjct: 430 HAVKDRLKELGYELDDEKLNAVFSLFRDLTKNKKRITDADLKALVTSSDEI 480

[Close]

BLAST of CSPI03G22250 vs. TAIR 10
Match: AT5G23020.1 (2-isopropylmalate synthase 2 )

HSP 1 Score: 540.0 bits (1390), Expect = 2.5e-153
Identity = 264/445 (59.33%), Postives = 351/445 (78.88%), Query Frame = 0


Query: 44  SFKTAAASAPHNVSPVY--SCLTES--IVSNSVPKSSI--RRPPYIPNRIPDPSYVRVFD 103
           SF +   + P++ + ++   C  ES  + +++     I  RRP YIPN++P  +YVRV D
Sbjct: 30  SFPSLRLTRPYDKATLFVSCCSAESKKVATSATDLKPIMERRPEYIPNKLPHKNYVRVLD 89

Query: 104 TTLRDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAV 163
           TTLRDGEQSPGA+LT  +KL+IARQLAKL VDI+E GFP +S+E+FEA+K IAK +GN V
Sbjct: 90  TTLRDGEQSPGAALTPPQKLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEV 149

Query: 164 DED-GYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIE 223
           DE+ GYVPVICG++RC ++DI   WEA+KYAKRPR+  F +TSEIHM++KL+KTKEEVIE
Sbjct: 150 DEETGYVPVICGIARCKKRDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIE 209

Query: 224 IARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFE 283
           +A N V++A+SLG  D++F  ED GR++++F+ +ILGE IKAGATT+   DTVG  MP E
Sbjct: 210 MAVNSVKYAKSLGFKDIQFGCEDGGRTEKDFICKILGESIKAGATTVGFADTVGINMPQE 269

Query: 284 FGKLIADIKSNTPGIENVIISSHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNA 343
           FG+L+A +  NTPG ++++ + HC NDLG+ATANT++G CAGARQVEVTINGIGER+GNA
Sbjct: 270 FGELVAYVIENTPGADDIVFAIHCHNDLGVATANTISGICAGARQVEVTINGIGERSGNA 329

Query: 344 SLEEVVMALQCRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHAS 403
            LEEVVMAL+CRGE ++ G++T I+SR I  TSKMV+E TG+ VQPHK IVG N F H S
Sbjct: 330 PLEEVVMALKCRGESLMDGVYTKIDSRQIMATSKMVQEHTGMYVQPHKPIVGDNCFVHES 389

Query: 404 GIHQDGMLKHKGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLD 463
           GIHQDG+LK++ TYEI++PED+G  +S ++GIVLGKLSGRHA+K  L ELGYE+  E  +
Sbjct: 390 GIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGRHAVKDRLKELGYEISDEKFN 449

Query: 464 NVFWRFKAVAEQKKRVTDADLRALV 482
           ++F R++ + + KKR+TDADL+ALV
Sbjct: 450 DIFSRYRELTKDKKRITDADLKALV 474

The following BLAST results are available for this feature:

BLAST of CSPI03G22250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C550	4.9e-271	80.83	2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q9LPR4	2.5e-267	83.73	2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
O04973	6.9e-249	76.84	2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1	[more]
O04974	2.2e-247	73.91	2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1	[more]
Q39891	5.1e-220	69.51	Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1	[more]

BLAST of CSPI03G22250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7C8	0.0e+00	99.84	2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1	[more]
A0A5D3BP53	0.0e+00	95.89	2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3C4S6	0.0e+00	95.56	2-isopropylmalate synthase OS=Cucumis melo OX=3656 GN=LOC103496846 PE=3 SV=1	[more]
A0A5A7UXZ0	0.0e+00	94.64	2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A6J1F3K1	0.0e+00	90.84	2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111441856 PE=3 SV...	[more]

BLAST of CSPI03G22250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651428.1	0.0e+00	99.84	2-isopropylmalate synthase 2, chloroplastic [Cucumis sativus] >KGN57885.1 hypoth...	[more]
TYK01483.1	0.0e+00	95.89	2-isopropylmalate synthase 2 [Cucumis melo var. makuwa]	[more]
XP_008457079.1	0.0e+00	95.56	PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cucumis melo]	[more]
KAA0060742.1	0.0e+00	94.64	2-isopropylmalate synthase 2 [Cucumis melo var. makuwa]	[more]
XP_038875433.1	0.0e+00	92.74	2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida]	[more]

BLAST of CSPI03G22250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74040.1	3.5e-272	80.83	2-isopropylmalate synthase 1	[more]
AT1G18500.1	1.8e-268	83.73	methylthioalkylmalate synthase-like 4	[more]
AT5G23010.1	2.3e-154	64.48	methylthioalkylmalate synthase 1	[more]
AT5G23020.1	2.5e-153	59.33	2-isopropylmalate synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013709	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	SMART	SM00917	LeuA_dimer_2	coord: 471..616 e-value: 7.5E-30 score: 115.2
IPR013709	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	PFAM	PF08502	LeuA_dimer	coord: 472..616 e-value: 1.9E-35 score: 121.7
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 89..378 e-value: 2.1E-99 score: 334.8
None	No IPR available	GENE3D	1.10.238.260		coord: 379..491 e-value: 1.4E-34 score: 120.8
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 73..621
None	No IPR available	PANTHER	PTHR10277:SF64	2-ISOPROPYLMALATE SYNTHASE 1 CHLOROPLASTIC	coord: 73..621
None	No IPR available	CDD	cd07940	DRE_TIM_IPMS	coord: 95..374 e-value: 4.09783E-179 score: 506.216
None	No IPR available	SUPERFAMILY	51569	Aldolase	coord: 90..390
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	GENE3D	3.30.160.270		coord: 498..616 e-value: 1.2E-23 score: 85.3
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	SUPERFAMILY	110921	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	coord: 494..615
IPR005671	2-isopropylmalate synthase, bacterial-type	TIGRFAM	TIGR00973	TIGR00973	coord: 93..613 e-value: 9.5E-199 score: 658.9
IPR005671	2-isopropylmalate synthase, bacterial-type	HAMAP	MF_01025	LeuA_type1	coord: 90..625 score: 39.418251
IPR000891	Pyruvate carboxyltransferase	PFAM	PF00682	HMGL-like	coord: 93..372 e-value: 1.8E-94 score: 316.2
IPR000891	Pyruvate carboxyltransferase	PROSITE	PS50991	PYR_CT	coord: 93..366 score: 42.174103
IPR002034	Alpha-isopropylmalate/homocitrate synthase, conserved site	PROSITE	PS00815	AIPM_HOMOCIT_SYNTH_1	coord: 100..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G22250.1	CSPI03G22250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009098	leucine biosynthetic process
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003852	2-isopropylmalate synthase activity
molecular_function	GO:0046912	acyltransferase, acyl groups converted into alkyl on transfer
molecular_function	GO:0003824	catalytic activity