CSPI03G22130 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G22130
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionethylene-responsive transcription factor CRF4-like
LocationChr3: 18532081 .. 18533835 (-)
RNA-Seq ExpressionCSPI03G22130
SyntenyCSPI03G22130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCATTGCAGCATTGCTATGGTGGTTGTTCACTTCTTTTCCTTCTGCCCAACACTCTAACACTTAACACTGCTTCTTTACTCTCTCTCTCTCTCTGCACTCTCAATAAATACTCATACAAACCTTCTTCTTCCTCTTCACAAACTCTCACTCTCAAAAACCCTTCTTCTTCTTCTTCTTCTTCTTCGTCTTCATCATCATCATCATCATCATCCCCTTTGCATATTTAATCCTACTCTCATCATCCCTTCAAAACACGACTAAAACACAGTCACACCAAACCCTGTTCCTCACTGTGGGCGTTTTTAGTTCCCTTTTTCCTCAGTGTGAATCCAAAATACGAAAAACTGTCTTCTTTTTTTTTCCTTTCACTTACTTTACCCTTTACCCTATTTTGCCTCCACTATCTCATCCTTCTATAAAATCCCCCATTGCTTCCGCAGTTTGATGCTGAATCTGCCATGGAGGACACTCTTTTTTCCCCTGTTAAATTCACAGAGCACCGTAACTTCACCAACAAATTTTCCGCTAAGAAACATCACCAATCCGAAAGTAGAGTCGTGCGGATTTCGGTAACCGACCCTGATGCCACGGATTCATCCAGTGATGAAGATGATTTTTTCGAGAGGCAGAGAGTCAAGAAATACATCAATGAGATCAAAATCCAAAGTGGGTGTCGGAATAATCTTTTACCCAGCTGTCGGAAGAGGCCTGCCGGTGACCGTTCTGAGTTTCGGCGCCAGGGGAAGGTGGTCCCTCCTACGAATGGGAAGAAATTTCGTGGTGTACGGCAACGGCCGTGGGGCAAATGGGCGGCGGAGATTAGAGATCCGGCTCGTCGTGTACGGCTCTGGCTTGGGACGTACGATACGGCGGAGGAAGCTGCGATGGTTTATGATAACGCTGCCATTCAGCTCCGTGGGCCGGATGCTTTAACCAACTTTGCAACACCTCCGCCGGTTCCGATGCCGGAGAAGGAAGTTGAGACGGCTAATATCCCATCTGTTTCCGGTTCTTATTACGATTCCAGTGAGGAGTCTCACAATCTATCATCGCCGACTTCGGTTCTTCACTTCAGAACACCGTCACCGGAAGAATCCGAAAAACCCCCAAAGTCCGATGACTTACAGAAACCGCCAGCGCCGTTTGTTGACGATCAATTTCACGAATGTCAAGGAGAAACTTCTTTTACTGAGGAGTACCACACGGAGTTCCCCAGATTCGACTATGATTTCAAGTTTCCAAGCCCCGAAGCGCCGATCTTCTTGGACGACCAGCCACTGTTCTTCGAAGACAGCATATGGAACGATGATTTCAGTGAAATTTTCACCAACTTGCCTGAAGATTTTGGGTCTCCATTGTTGTCTTCTTCGATCGGTCAAGGGGGCGATGACTATTTCCAAGATATTTTAATGGGGTCAGACCCTTTGGTAGTTTTGTAAGCGGCGGAGCATTGGCTGCCGACGACGGCGGACCACCGTTATGTCGGCCGAATTTTGTTTTCCATGGTCGCATTTCGCAAAAATAAAAGGGCCAATTTTGCGAATTTTGCTTTTTTTTGGTATACTTTTCCTTACCTGTTTACATATTCTGGGAAGTTGAGTGGACCAGTTTTGCTAGAGATATTTATTCTTTTTATTTCCTTTTTTATTTTTATTTTTTTAAATTTTGATTTAAGTCAATAATTAGGCTTTGTCATTTAAGAGTGTATAACGTGGAAGAGCTTTGTATAATTATATATCTCCAAATTTTGTC

mRNA sequence

TTCCATTGCAGCATTGCTATGGTGGTTGTTCACTTCTTTTCCTTCTGCCCAACACTCTAACACTTAACACTGCTTCTTTACTCTCTCTCTCTCTCTGCACTCTCAATAAATACTCATACAAACCTTCTTCTTCCTCTTCACAAACTCTCACTCTCAAAAACCCTTCTTCTTCTTCTTCTTCTTCTTCGTCTTCATCATCATCATCATCATCATCCCCTTTGCATATTTAATCCTACTCTCATCATCCCTTCAAAACACGACTAAAACACAGTCACACCAAACCCTGTTCCTCACTGTGGGCGTTTTTAGTTCCCTTTTTCCTCAGTGTGAATCCAAAATACGAAAAACTGTCTTCTTTTTTTTTCCTTTCACTTACTTTACCCTTTACCCTATTTTGCCTCCACTATCTCATCCTTCTATAAAATCCCCCATTGCTTCCGCAGTTTGATGCTGAATCTGCCATGGAGGACACTCTTTTTTCCCCTGTTAAATTCACAGAGCACCGTAACTTCACCAACAAATTTTCCGCTAAGAAACATCACCAATCCGAAAGTAGAGTCGTGCGGATTTCGGTAACCGACCCTGATGCCACGGATTCATCCAGTGATGAAGATGATTTTTTCGAGAGGCAGAGAGTCAAGAAATACATCAATGAGATCAAAATCCAAAGTGGGTGTCGGAATAATCTTTTACCCAGCTGTCGGAAGAGGCCTGCCGGTGACCGTTCTGAGTTTCGGCGCCAGGGGAAGGTGGTCCCTCCTACGAATGGGAAGAAATTTCGTGGTGTACGGCAACGGCCGTGGGGCAAATGGGCGGCGGAGATTAGAGATCCGGCTCGTCGTGTACGGCTCTGGCTTGGGACGTACGATACGGCGGAGGAAGCTGCGATGGTTTATGATAACGCTGCCATTCAGCTCCGTGGGCCGGATGCTTTAACCAACTTTGCAACACCTCCGCCGGTTCCGATGCCGGAGAAGGAAGTTGAGACGGCTAATATCCCATCTGTTTCCGGTTCTTATTACGATTCCAGTGAGGAGTCTCACAATCTATCATCGCCGACTTCGGTTCTTCACTTCAGAACACCGTCACCGGAAGAATCCGAAAAACCCCCAAAGTCCGATGACTTACAGAAACCGCCAGCGCCGTTTGTTGACGATCAATTTCACGAATGTCAAGGAGAAACTTCTTTTACTGAGGAGTACCACACGGAGTTCCCCAGATTCGACTATGATTTCAAGTTTCCAAGCCCCGAAGCGCCGATCTTCTTGGACGACCAGCCACTGTTCTTCGAAGACAGCATATGGAACGATGATTTCAGTGAAATTTTCACCAACTTGCCTGAAGATTTTGGGTCTCCATTGTTGTCTTCTTCGATCGGTCAAGGGGGCGATGACTATTTCCAAGATATTTTAATGGGGTCAGACCCTTTGGTAGTTTTGTAAGCGGCGGAGCATTGGCTGCCGACGACGGCGGACCACCGTTATGTCGGCCGAATTTTGTTTTCCATGGTCGCATTTCGCAAAAATAAAAGGGCCAATTTTGCGAATTTTGCTTTTTTTTGGTATACTTTTCCTTACCTGTTTACATATTCTGGGAAGTTGAGTGGACCAGTTTTGCTAGAGATATTTATTCTTTTTATTTCCTTTTTTATTTTTATTTTTTTAAATTTTGATTTAAGTCAATAATTAGGCTTTGTCATTTAAGAGTGTATAACGTGGAAGAGCTTTGTATAATTATATATCTCCAAATTTTGTC

Coding sequence (CDS)

ATGGAGGACACTCTTTTTTCCCCTGTTAAATTCACAGAGCACCGTAACTTCACCAACAAATTTTCCGCTAAGAAACATCACCAATCCGAAAGTAGAGTCGTGCGGATTTCGGTAACCGACCCTGATGCCACGGATTCATCCAGTGATGAAGATGATTTTTTCGAGAGGCAGAGAGTCAAGAAATACATCAATGAGATCAAAATCCAAAGTGGGTGTCGGAATAATCTTTTACCCAGCTGTCGGAAGAGGCCTGCCGGTGACCGTTCTGAGTTTCGGCGCCAGGGGAAGGTGGTCCCTCCTACGAATGGGAAGAAATTTCGTGGTGTACGGCAACGGCCGTGGGGCAAATGGGCGGCGGAGATTAGAGATCCGGCTCGTCGTGTACGGCTCTGGCTTGGGACGTACGATACGGCGGAGGAAGCTGCGATGGTTTATGATAACGCTGCCATTCAGCTCCGTGGGCCGGATGCTTTAACCAACTTTGCAACACCTCCGCCGGTTCCGATGCCGGAGAAGGAAGTTGAGACGGCTAATATCCCATCTGTTTCCGGTTCTTATTACGATTCCAGTGAGGAGTCTCACAATCTATCATCGCCGACTTCGGTTCTTCACTTCAGAACACCGTCACCGGAAGAATCCGAAAAACCCCCAAAGTCCGATGACTTACAGAAACCGCCAGCGCCGTTTGTTGACGATCAATTTCACGAATGTCAAGGAGAAACTTCTTTTACTGAGGAGTACCACACGGAGTTCCCCAGATTCGACTATGATTTCAAGTTTCCAAGCCCCGAAGCGCCGATCTTCTTGGACGACCAGCCACTGTTCTTCGAAGACAGCATATGGAACGATGATTTCAGTGAAATTTTCACCAACTTGCCTGAAGATTTTGGGTCTCCATTGTTGTCTTCTTCGATCGGTCAAGGGGGCGATGACTATTTCCAAGATATTTTAATGGGGTCAGACCCTTTGGTAGTTTTGTAA

Protein sequence

MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVKKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIPSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPLLSSSIGQGGDDYFQDILMGSDPLVVL*
Homology
BLAST of CSPI03G22130 vs. ExPASy Swiss-Prot
Match: Q9SUE3 (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-48
Identity = 138/333 (41.44%), Postives = 193/333 (57.96%), Query Frame = 0

Query: 8   PVKFTEHRNFTNKFSAKKHHQ------SESRVVRISVTDPDATDSSSDEDDF-FERQRVK 67
           PVK+TEH+    K++ K   +        +R+VR+S+TD DATDSSSDE++F F R+RVK
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVK 70

Query: 68  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPT----NGKKFRGVRQRPWGK 127
           + INEI+++    ++        P  DR        V  P+    N KK+RGVRQRPWGK
Sbjct: 71  RLINEIRVEPS--SSSTGDVSASPTKDRKRINVDSTVQKPSVSGQNQKKYRGVRQRPWGK 130

Query: 128 WAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAT-PPPVPMPEKEVE 187
           WAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNF   P P P+ E+E E
Sbjct: 131 WAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQE 190

Query: 188 TANIPSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEES----EKPPKSDDLQKPPAPFVD 247
             +  SVS S  +S ++S +LSSPTSVL+++T   EE      KP K + L+    P   
Sbjct: 191 PESNMSVSIS--ESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQEPI-- 250

Query: 248 DQFHECQGETSFTEE-YHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIF- 307
             +H  +G T+  ++ +  +    D  F    P+  IF  DQP+       ND F+++  
Sbjct: 251 -SWHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFNDLML 310

Query: 308 --TNLPEDFGSPL--LSSSIGQGGDDYFQDILM 319
             +N  E + S +  + SS     D    D+L+
Sbjct: 311 FDSNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

BLAST of CSPI03G22130 vs. ExPASy Swiss-Prot
Match: Q9FK12 (Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN=CRF3 PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.7e-46
Identity = 152/359 (42.34%), Postives = 197/359 (54.87%), Query Frame = 0

Query: 6   FSPVKFTEHR----NFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDD---FFERQR 65
           F P+K+TEH+     +T K S K       + V I  TDPDATDSSSDED+    F R+R
Sbjct: 7   FRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRR 66

Query: 66  VKKYINEIKIQSGCRN---NLLPSCRKR----------PAGDRSEFRRQGKVVPPTNGKK 125
           VK+++NEI ++  C N    +    RKR          PA  R   R   KV      KK
Sbjct: 67  VKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQ--RPNNKVSVSGQIKK 126

Query: 126 FRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP 185
           FRGVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF+ PP
Sbjct: 127 FRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPP 186

Query: 186 --------PVPMPEKEVETANIPSVSGSYYDSSEES-HNLSSPTSVLHFRTPSPEESEKP 245
                   P P+ E++      P+ + S  +S+EE   +LSSPTSVL+ R+   ++ ++P
Sbjct: 187 QEEEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQP 246

Query: 246 PKSDDLQKPP-----APFVDDQFHECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDD 305
            KS    KP      AP+    F+   GE+   + +  + P  D  F    PE  IF  D
Sbjct: 247 FKS---AKPEPGVSNAPWWHTGFNTGLGESD--DSFPLDTPFLDNYFNESPPEMSIF--D 306

Query: 306 QP---LFFE-DSIWNDDFSEIFTNLPEDFGSPLLSSSIGQGGDDY--FQDILMGSDPLV 325
           QP   +F E D I+ND           +    L SSSI   G  +  F D L+ SD LV
Sbjct: 307 QPMDQIFCENDDIFNDML--FLGGETMNIEDELTSSSIKDMGSTFSDFDDSLI-SDLLV 353

BLAST of CSPI03G22130 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.3e-46
Identity = 141/358 (39.39%), Postives = 178/358 (49.72%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDD------FFERQRVKKY 68
           +KFTEH+  T    ++  +  ++R++RISVTDPDATDSSSD+++        +R+RVKK+
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  INEIKIQSG------CRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNG-KKFRGVRQRPWG 128
           +NE+ + SG      C        +KR     S           T G KKFRGVRQRPWG
Sbjct: 72  VNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWG 131

Query: 129 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP--------PV 188
           KWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+  P        P 
Sbjct: 132 KWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPP 191

Query: 189 PMPEKEVETANIPS----VSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQ 248
           P P K+ +  N  S     + S    S  +  L SP SVL                    
Sbjct: 192 PSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLR------------------- 251

Query: 249 KPPAPFVDDQFHECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWND 308
              +PF  D+F               E         F    AP+F DD    F  S+  D
Sbjct: 252 ---SPFAVDEFSGISSSPVAAVVVKEEPSMTTVSETFSDFSAPLFSDDDVFDFRSSVVPD 311

Query: 309 DF------SEIFT-------NLPEDFGSPLLSSSIGQGGDDYFQDI--LMGSDPLVVL 327
                    ++FT       N   DFGS L S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 YLGGDLFGEDLFTADMCTDMNFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of CSPI03G22130 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.1e-36
Identity = 134/315 (42.54%), Postives = 166/315 (52.70%), Query Frame = 0

Query: 32  RVVRISVTDPDATDSSSDED---DF----FERQRVKKYINEIKIQSGCRNNLLPSCRKRP 91
           R++RISVTDP ATDSSSDE+   DF     +R+RVKKY+ E+ + S   +   P  +KR 
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDKEKPMKKKRK 70

Query: 92  AGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMV 151
               +       VV  T  +KFRGVRQRPWGKWAAEIRDP+RRVR+WLGT+DTAEEAA+V
Sbjct: 71  KRVVTV-----PVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIV 130

Query: 152 YDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIPSVSGSYYDSSEESHNLSSPTSVLH 211
           YDNAAIQLRGP+A  NF  PPPV    +E  T  +  VS       E    L SP SVL 
Sbjct: 131 YDNAAIQLRGPNAELNF-PPPPVTENVEEAST-EVKGVSDFIIGGGE---CLRSPVSVLE 190

Query: 212 FRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGETSFTEEYHTEFPRFD-YDFKFPSP 271
               SP   E     ++       FV           S  E    + P F+  DF     
Sbjct: 191 ----SPFSGESTAVKEE-------FVG---------VSTAEIVVKKEPSFNGSDF----- 250

Query: 272 EAPIFLDDQPLFFEDSIWNDDFSEIF-TNLPED--FGSPLLSSSIGQGG-------DDYF 327
            AP+F DD    F  S+      ++F  NL  D  FGS       G G        +D+F
Sbjct: 251 SAPLFSDDDVFGFSTSMSESFGGDLFGDNLFADMSFGS---GFGFGSGSGFSSWHVEDHF 287

BLAST of CSPI03G22130 vs. ExPASy Swiss-Prot
Match: Q9M374 (Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN=CRF6 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 121/313 (38.66%), Postives = 162/313 (51.76%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDD---FFERQRVKKYINE 68
           VKFTE+R  TN   A        R+VRI+VTDP ATDSSSD+DD        RVK+Y+ E
Sbjct: 8   VKFTENRTVTN--VAATPSNGSPRLVRITVTDPFATDSSSDDDDNNNVTVVPRVKRYVKE 67

Query: 69  IKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAEIRDPA 128
           I+   G  ++   + RK    +      +  V      KK+RGVRQRPWGK+AAEIRDP+
Sbjct: 68  IRFCQG-ESSSSTAARKGKHKEEESVVVEDDVSTSVKPKKYRGVRQRPWGKFAAEIRDPS 127

Query: 129 RRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIPSVSGS 188
            R R+WLGT+ TAEEAA+ YD AAI L+GP ALTNF T PP P P  +++T     VS  
Sbjct: 128 SRTRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLT-PPTPTPVIDLQT-----VSAC 187

Query: 189 YYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGETSFTE 248
            Y   +   +L SPTSVL F     EE+E   ++ +L       V  +  E      F +
Sbjct: 188 DY-GRDSRQSLHSPTSVLRFNV--NEETEHEIEAIELSPERKSTVIKEEEESSAGLVFPD 247

Query: 249 EYHTEFPRFDYDFKFPSPEAP--IFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPLLSS 308
            Y         +  + +  AP  +FLD++       + N + S+   N  EDF   L+  
Sbjct: 248 PYLLPDLSLAGECFWDTEIAPDLLFLDEETKIQSTLLPNTEVSKQGENETEDFEFGLIDD 307

Query: 309 SIGQGGD-DYFQD 316
                 D D+F D
Sbjct: 308 FESSPWDVDHFFD 308

BLAST of CSPI03G22130 vs. ExPASy TrEMBL
Match: A0A0A0LB30 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=4 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.0e-187
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE
Sbjct: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL
Sbjct: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300

Query: 301 LSSSIGQGGDDYFQDILMGSDPLVVL 327
           LSSSIGQGGDDYFQDILMGSDPLVVL
Sbjct: 301 LSSSIGQGGDDYFQDILMGSDPLVVL 326

BLAST of CSPI03G22130 vs. ExPASy TrEMBL
Match: A0A5D3BJ86 (Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold63471G00010 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 7.8e-183
Identity = 321/327 (98.17%), Postives = 323/327 (98.78%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP VPMPEKEVET NIP
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNIP 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE
Sbjct: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           TSF+EEYHTEFPRFDYDFKFPSPE+PIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL
Sbjct: 241 TSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300

Query: 301 L-SSSIGQGGDDYFQDILMGSDPLVVL 327
           L SSSIGQGGDDYFQDIL GSDPLVVL
Sbjct: 301 LSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of CSPI03G22130 vs. ExPASy TrEMBL
Match: A0A1S3AY55 (ethylene-responsive transcription factor CRF4-like OS=Cucumis melo OX=3656 GN=LOC103484149 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 7.8e-183
Identity = 321/327 (98.17%), Postives = 323/327 (98.78%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP VPMPEKEVET NIP
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNIP 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE
Sbjct: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           TSF+EEYHTEFPRFDYDFKFPSPE+PIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL
Sbjct: 241 TSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300

Query: 301 L-SSSIGQGGDDYFQDILMGSDPLVVL 327
           L SSSIGQGGDDYFQDIL GSDPLVVL
Sbjct: 301 LSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of CSPI03G22130 vs. ExPASy TrEMBL
Match: A0A6J1E4E1 (ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662 GN=LOC111430503 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 8.1e-156
Identity = 278/326 (85.28%), Postives = 297/326 (91.10%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLF PVK TEHRNFT+KFS KKHHQSESRVVRISVTDPDATDSSSDE++ FERQRVK
Sbjct: 1   MEDTLFCPVKVTEHRNFTSKFSLKKHHQSESRVVRISVTDPDATDSSSDEEELFERQRVK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRN+LLPSCRKR AG++S+ RR GKVVP TNGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNSLLPSCRKRSAGEQSDSRRSGKVVPATNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATP  VPMPEKEVE ANI 
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPSSVPMPEKEVEVANIA 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           SVSGSYYDSSEESHNLSSPTSVLHFRT SPEE+EKP K D+ Q+PP PF DDQFHE Q +
Sbjct: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTSSPEETEKPQKPDESQQPPPPFADDQFHERQVK 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           T+ +EEY+TEFPRFD  FK P PE PIF+D+QPLFFE+SIWN+DFSE FTNLPEDFG+PL
Sbjct: 241 TTLSEEYYTEFPRFDDVFKLPIPEPPIFVDEQPLFFEESIWNEDFSEFFTNLPEDFGTPL 300

Query: 301 LSSSIGQGGDDYFQDILMGSDPLVVL 327
           LSS I QGGDDYFQDILMGSDPLVVL
Sbjct: 301 LSSPISQGGDDYFQDILMGSDPLVVL 326

BLAST of CSPI03G22130 vs. ExPASy TrEMBL
Match: A0A6J1J7T6 (ethylene-responsive transcription factor CRF4-like OS=Cucurbita maxima OX=3661 GN=LOC111483359 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 2.9e-153
Identity = 273/326 (83.74%), Postives = 295/326 (90.49%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLF PVK TEHRNFT+KFS KKHHQSESRVVRISVTDPDATDSSSDE++ FERQR+K
Sbjct: 1   MEDTLFCPVKVTEHRNFTSKFSLKKHHQSESRVVRISVTDPDATDSSSDEEELFERQRMK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRN+LLPSCRKR AG++S+ RR GKVVP TNGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNSLLPSCRKRSAGEQSDSRRSGKVVPATNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATP  VPMPEKEVE ANI 
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPSSVPMPEKEVEVANIA 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           SVSGSYYDSSEESHNLSSPTSVLHFRT SPEE+EKP K D+ Q+PP PF DDQF ECQ +
Sbjct: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTTSPEETEKPQKPDESQQPPPPFADDQFPECQVK 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           T+ + EY+TEFPRFD  FK P  E+PIF+D+QPLFFE+SIW +DFSE FTNLPEDFG+PL
Sbjct: 241 TTISSEYYTEFPRFDDVFKLPISESPIFVDEQPLFFEESIWKEDFSEFFTNLPEDFGTPL 300

Query: 301 LSSSIGQGGDDYFQDILMGSDPLVVL 327
           LSS I QGGD+YFQDILMGSDPLVVL
Sbjct: 301 LSSPISQGGDEYFQDILMGSDPLVVL 326

BLAST of CSPI03G22130 vs. NCBI nr
Match: XP_004149454.1 (ethylene-responsive transcription factor CRF4 [Cucumis sativus] >KGN57872.1 hypothetical protein Csa_011137 [Cucumis sativus])

HSP 1 Score: 663.7 bits (1711), Expect = 8.3e-187
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE
Sbjct: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL
Sbjct: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300

Query: 301 LSSSIGQGGDDYFQDILMGSDPLVVL 327
           LSSSIGQGGDDYFQDILMGSDPLVVL
Sbjct: 301 LSSSIGQGGDDYFQDILMGSDPLVVL 326

BLAST of CSPI03G22130 vs. NCBI nr
Match: XP_008439327.1 (PREDICTED: ethylene-responsive transcription factor CRF4-like [Cucumis melo] >KAA0033741.1 ethylene-responsive transcription factor CRF4-like [Cucumis melo var. makuwa] >TYJ99880.1 ethylene-responsive transcription factor CRF4-like [Cucumis melo var. makuwa])

HSP 1 Score: 649.4 bits (1674), Expect = 1.6e-182
Identity = 321/327 (98.17%), Postives = 323/327 (98.78%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP VPMPEKEVET NIP
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNIP 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE
Sbjct: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           TSF+EEYHTEFPRFDYDFKFPSPE+PIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL
Sbjct: 241 TSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300

Query: 301 L-SSSIGQGGDDYFQDILMGSDPLVVL 327
           L SSSIGQGGDDYFQDIL GSDPLVVL
Sbjct: 301 LSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of CSPI03G22130 vs. NCBI nr
Match: XP_038900136.1 (ethylene-responsive transcription factor CRF4-like [Benincasa hispida])

HSP 1 Score: 630.9 bits (1626), Expect = 5.9e-177
Identity = 307/326 (94.17%), Postives = 317/326 (97.24%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLFSPVKFTEHRNFTNKFSAKKHH SESRVVRISVTDPDATDSSSDEDD+FERQRVK
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHHSESRVVRISVTDPDATDSSSDEDDYFERQRVK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRNNLLPSCRKRPAG+RS+F+RQGKV PP NGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNNLLPSCRKRPAGERSDFQRQGKVAPPMNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPV MPEKEVE ANIP
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVSMPEKEVEMANIP 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           SVSGSYYDS EESHNLSSPTSVLHFRTPSPEESEKPPKSD++Q PPAPF DDQFHECQGE
Sbjct: 181 SVSGSYYDSCEESHNLSSPTSVLHFRTPSPEESEKPPKSDEVQNPPAPFTDDQFHECQGE 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           TSF+EE+HTEFPRFDYDFKFPSPE+PIFLDDQPLFFEDSIWN+DFSEIFTNLPEDFGSPL
Sbjct: 241 TSFSEEFHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNEDFSEIFTNLPEDFGSPL 300

Query: 301 LSSSIGQGGDDYFQDILMGSDPLVVL 327
           LSSSI QGGDDYFQDILMGSDPLVVL
Sbjct: 301 LSSSISQGGDDYFQDILMGSDPLVVL 326

BLAST of CSPI03G22130 vs. NCBI nr
Match: KAG6576679.1 (Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014728.1 Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 567.4 bits (1461), Expect = 8.1e-158
Identity = 280/326 (85.89%), Postives = 299/326 (91.72%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLF PVK TEHRNFT+KFS KKHHQSESRVVRISVTDPDATDSSSDE++ FERQRVK
Sbjct: 1   MEDTLFCPVKVTEHRNFTSKFSLKKHHQSESRVVRISVTDPDATDSSSDEEELFERQRVK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRN+LLPSCRKR AG++S+ RR GKVVP TNGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNSLLPSCRKRSAGEQSDSRRSGKVVPATNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATP  VPMPEKEVE ANI 
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPSSVPMPEKEVEVANIA 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEE+EKP K D+ Q+PP PF DDQFHECQ +
Sbjct: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEETEKPQKPDESQQPPPPFADDQFHECQVK 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           T+ +EEY+TEFPRFD  FK P PE PIF+D+QPLFFE+SIWN+DFSE FTNLPEDFG+PL
Sbjct: 241 TTLSEEYYTEFPRFDDVFKLPIPEPPIFVDEQPLFFEESIWNEDFSEFFTNLPEDFGTPL 300

Query: 301 LSSSIGQGGDDYFQDILMGSDPLVVL 327
           LSS I QGGDDYFQDILMGSDPLVVL
Sbjct: 301 LSSPISQGGDDYFQDILMGSDPLVVL 326

BLAST of CSPI03G22130 vs. NCBI nr
Match: XP_023552792.1 (ethylene-responsive transcription factor CRF4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 564.7 bits (1454), Expect = 5.2e-157
Identity = 278/326 (85.28%), Postives = 299/326 (91.72%), Query Frame = 0

Query: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDDFFERQRVK 60
           MEDTLF PVK TEHRNFT+KFS KK+HQSESRVVRISVTDPDATDSSSDE++ FERQRVK
Sbjct: 1   MEDTLFCPVKLTEHRNFTSKFSLKKYHQSESRVVRISVTDPDATDSSSDEEELFERQRVK 60

Query: 61  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAE 120
           KYINEIKIQSGCRN+LLPSCRKR AG++S+ RR GKVVP TNGKKFRGVRQRPWGKWAAE
Sbjct: 61  KYINEIKIQSGCRNSLLPSCRKRSAGEQSDSRRSGKVVPATNGKKFRGVRQRPWGKWAAE 120

Query: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIP 180
           IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATP PVPMPEKEVE ANI 
Sbjct: 121 IRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPSPVPMPEKEVEVANIA 180

Query: 181 SVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGE 240
           S SGSYYDSSEESHNLSSPTSVLHFRTPSPEE+EKP K D+ Q+PP PF DDQFHECQ +
Sbjct: 181 SGSGSYYDSSEESHNLSSPTSVLHFRTPSPEETEKPQKPDESQQPPPPFADDQFHECQVK 240

Query: 241 TSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNLPEDFGSPL 300
           T+ + EY+TEFPRFD  FK P PE+PIF+D+QPLFFE+SIWN+DFSE FTNLPEDFG+PL
Sbjct: 241 TTISSEYYTEFPRFDDVFKLPIPESPIFVDEQPLFFEESIWNEDFSEFFTNLPEDFGTPL 300

Query: 301 LSSSIGQGGDDYFQDILMGSDPLVVL 327
           LSS I QGGDDYFQDILMGSDPLVVL
Sbjct: 301 LSSPISQGGDDYFQDILMGSDPLVVL 326

BLAST of CSPI03G22130 vs. TAIR 10
Match: AT4G27950.1 (cytokinin response factor 4 )

HSP 1 Score: 194.9 bits (494), Expect = 1.0e-49
Identity = 138/333 (41.44%), Postives = 193/333 (57.96%), Query Frame = 0

Query: 8   PVKFTEHRNFTNKFSAKKHHQ------SESRVVRISVTDPDATDSSSDEDDF-FERQRVK 67
           PVK+TEH+    K++ K   +        +R+VR+S+TD DATDSSSDE++F F R+RVK
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVK 70

Query: 68  KYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPT----NGKKFRGVRQRPWGK 127
           + INEI+++    ++        P  DR        V  P+    N KK+RGVRQRPWGK
Sbjct: 71  RLINEIRVEPS--SSSTGDVSASPTKDRKRINVDSTVQKPSVSGQNQKKYRGVRQRPWGK 130

Query: 128 WAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAT-PPPVPMPEKEVE 187
           WAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNF   P P P+ E+E E
Sbjct: 131 WAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQE 190

Query: 188 TANIPSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEES----EKPPKSDDLQKPPAPFVD 247
             +  SVS S  +S ++S +LSSPTSVL+++T   EE      KP K + L+    P   
Sbjct: 191 PESNMSVSIS--ESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQEPI-- 250

Query: 248 DQFHECQGETSFTEE-YHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIF- 307
             +H  +G T+  ++ +  +    D  F    P+  IF  DQP+       ND F+++  
Sbjct: 251 -SWHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFNDLML 310

Query: 308 --TNLPEDFGSPL--LSSSIGQGGDDYFQDILM 319
             +N  E + S +  + SS     D    D+L+
Sbjct: 311 FDSNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

BLAST of CSPI03G22130 vs. TAIR 10
Match: AT5G53290.1 (cytokinin response factor 3 )

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-47
Identity = 152/359 (42.34%), Postives = 197/359 (54.87%), Query Frame = 0

Query: 6   FSPVKFTEHR----NFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDD---FFERQR 65
           F P+K+TEH+     +T K S K       + V I  TDPDATDSSSDED+    F R+R
Sbjct: 7   FRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRR 66

Query: 66  VKKYINEIKIQSGCRN---NLLPSCRKR----------PAGDRSEFRRQGKVVPPTNGKK 125
           VK+++NEI ++  C N    +    RKR          PA  R   R   KV      KK
Sbjct: 67  VKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQ--RPNNKVSVSGQIKK 126

Query: 126 FRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP 185
           FRGVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF+ PP
Sbjct: 127 FRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPP 186

Query: 186 --------PVPMPEKEVETANIPSVSGSYYDSSEES-HNLSSPTSVLHFRTPSPEESEKP 245
                   P P+ E++      P+ + S  +S+EE   +LSSPTSVL+ R+   ++ ++P
Sbjct: 187 QEEEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQP 246

Query: 246 PKSDDLQKPP-----APFVDDQFHECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDD 305
            KS    KP      AP+    F+   GE+   + +  + P  D  F    PE  IF  D
Sbjct: 247 FKS---AKPEPGVSNAPWWHTGFNTGLGESD--DSFPLDTPFLDNYFNESPPEMSIF--D 306

Query: 306 QP---LFFE-DSIWNDDFSEIFTNLPEDFGSPLLSSSIGQGGDDY--FQDILMGSDPLV 325
           QP   +F E D I+ND           +    L SSSI   G  +  F D L+ SD LV
Sbjct: 307 QPMDQIFCENDDIFNDML--FLGGETMNIEDELTSSSIKDMGSTFSDFDDSLI-SDLLV 353

BLAST of CSPI03G22130 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 187.6 bits (475), Expect = 1.6e-47
Identity = 141/358 (39.39%), Postives = 178/358 (49.72%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDD------FFERQRVKKY 68
           +KFTEH+  T    ++  +  ++R++RISVTDPDATDSSSD+++        +R+RVKK+
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  INEIKIQSG------CRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNG-KKFRGVRQRPWG 128
           +NE+ + SG      C        +KR     S           T G KKFRGVRQRPWG
Sbjct: 72  VNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWG 131

Query: 129 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP--------PV 188
           KWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+  P        P 
Sbjct: 132 KWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPP 191

Query: 189 PMPEKEVETANIPS----VSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQ 248
           P P K+ +  N  S     + S    S  +  L SP SVL                    
Sbjct: 192 PSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLR------------------- 251

Query: 249 KPPAPFVDDQFHECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWND 308
              +PF  D+F               E         F    AP+F DD    F  S+  D
Sbjct: 252 ---SPFAVDEFSGISSSPVAAVVVKEEPSMTTVSETFSDFSAPLFSDDDVFDFRSSVVPD 311

Query: 309 DF------SEIFT-------NLPEDFGSPLLSSSIGQGGDDYFQDI--LMGSDPLVVL 327
                    ++FT       N   DFGS L S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 YLGGDLFGEDLFTADMCTDMNFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of CSPI03G22130 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 187.6 bits (475), Expect = 1.6e-47
Identity = 141/358 (39.39%), Postives = 178/358 (49.72%), Query Frame = 0

Query: 9   VKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDEDD------FFERQRVKKY 68
           +KFTEH+  T    ++  +  ++R++RISVTDPDATDSSSD+++        +R+RVKK+
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  INEIKIQSG------CRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNG-KKFRGVRQRPWG 128
           +NE+ + SG      C        +KR     S           T G KKFRGVRQRPWG
Sbjct: 72  VNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWG 131

Query: 129 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPP--------PV 188
           KWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF+  P        P 
Sbjct: 132 KWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPP 191

Query: 189 PMPEKEVETANIPS----VSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQ 248
           P P K+ +  N  S     + S    S  +  L SP SVL                    
Sbjct: 192 PSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLR------------------- 251

Query: 249 KPPAPFVDDQFHECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWND 308
              +PF  D+F               E         F    AP+F DD    F  S+  D
Sbjct: 252 ---SPFAVDEFSGISSSPVAAVVVKEEPSMTTVSETFSDFSAPLFSDDDVFDFRSSVVPD 311

Query: 309 DF------SEIFT-------NLPEDFGSPLLSSSIGQGGDDYFQDI--LMGSDPLVVL 327
                    ++FT       N   DFGS L S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 YLGGDLFGEDLFTADMCTDMNFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of CSPI03G22130 vs. TAIR 10
Match: AT4G11140.1 (cytokinin response factor 1 )

HSP 1 Score: 154.5 bits (389), Expect = 1.5e-37
Identity = 134/315 (42.54%), Postives = 166/315 (52.70%), Query Frame = 0

Query: 32  RVVRISVTDPDATDSSSDED---DF----FERQRVKKYINEIKIQSGCRNNLLPSCRKRP 91
           R++RISVTDP ATDSSSDE+   DF     +R+RVKKY+ E+ + S   +   P  +KR 
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDKEKPMKKKRK 70

Query: 92  AGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMV 151
               +       VV  T  +KFRGVRQRPWGKWAAEIRDP+RRVR+WLGT+DTAEEAA+V
Sbjct: 71  KRVVTV-----PVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIV 130

Query: 152 YDNAAIQLRGPDALTNFATPPPVPMPEKEVETANIPSVSGSYYDSSEESHNLSSPTSVLH 211
           YDNAAIQLRGP+A  NF  PPPV    +E  T  +  VS       E    L SP SVL 
Sbjct: 131 YDNAAIQLRGPNAELNF-PPPPVTENVEEAST-EVKGVSDFIIGGGE---CLRSPVSVLE 190

Query: 212 FRTPSPEESEKPPKSDDLQKPPAPFVDDQFHECQGETSFTEEYHTEFPRFD-YDFKFPSP 271
               SP   E     ++       FV           S  E    + P F+  DF     
Sbjct: 191 ----SPFSGESTAVKEE-------FVG---------VSTAEIVVKKEPSFNGSDF----- 250

Query: 272 EAPIFLDDQPLFFEDSIWNDDFSEIF-TNLPED--FGSPLLSSSIGQGG-------DDYF 327
            AP+F DD    F  S+      ++F  NL  D  FGS       G G        +D+F
Sbjct: 251 SAPLFSDDDVFGFSTSMSESFGGDLFGDNLFADMSFGS---GFGFGSGSGFSSWHVEDHF 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUE31.4e-4841.44Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FK121.7e-4642.34Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUQ22.3e-4639.39Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN... [more]
O825032.1e-3642.54Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9M3741.5e-3438.66Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0LB304.0e-187100.00AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=... [more]
A0A5D3BJ867.8e-18398.17Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa O... [more]
A0A1S3AY557.8e-18398.17ethylene-responsive transcription factor CRF4-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1E4E18.1e-15685.28ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1J7T62.9e-15383.74ethylene-responsive transcription factor CRF4-like OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
XP_004149454.18.3e-187100.00ethylene-responsive transcription factor CRF4 [Cucumis sativus] >KGN57872.1 hypo... [more]
XP_008439327.11.6e-18298.17PREDICTED: ethylene-responsive transcription factor CRF4-like [Cucumis melo] >KA... [more]
XP_038900136.15.9e-17794.17ethylene-responsive transcription factor CRF4-like [Benincasa hispida][more]
KAG6576679.18.1e-15885.89Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma s... [more]
XP_023552792.15.2e-15785.28ethylene-responsive transcription factor CRF4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G27950.11.0e-4941.44cytokinin response factor 4 [more]
AT5G53290.11.2e-4742.34cytokinin response factor 3 [more]
AT4G23750.11.6e-4739.39cytokinin response factor 2 [more]
AT4G23750.21.6e-4739.39cytokinin response factor 2 [more]
AT4G11140.11.5e-3742.54cytokinin response factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 106..117
score: 70.91
coord: 128..144
score: 61.5
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 105..168
e-value: 5.4E-37
score: 138.9
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 105..154
e-value: 8.4E-14
score: 51.6
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 105..162
score: 23.762146
IPR001471AP2/ERF domainCDDcd00018AP2coord: 104..162
e-value: 7.67256E-34
score: 116.987
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 104..163
e-value: 3.7E-26
score: 93.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 178..207
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 170..228
NoneNo IPR availablePANTHERPTHR31194:SF137ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF4-LIKEcoord: 6..217
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 6..217
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 105..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G22130.1CSPI03G22130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity