CSPI03G22010 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G22010
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	dihydrofolate synthetase isoform X1
Location	Chr3: 18456965 .. 18462923 (-)
RNA-Seq Expression	CSPI03G22010
Synteny	CSPI03G22010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTCTTCAAATATCATCACCACTGTCGCCCGCAGATTCTCGGTAGCCTTCTCCTAAATCATTTCATTGGCGAATGCCCTTCTATCAGTTCCCGATTTGGGTCCAAACAATGCTTCGCCACTCACTCAGAAGACCAACACATGACCCAATTCATGGAATATTTAGATTCCCTGAAAAACTACGAGAAATTGGGTGTACCCAGAGGTTCAGGCACTGATTCTGATGATGGGTTCGATCTGGGAAGGATGAGAAGGCTGATGGAACGACTGGGTAATCCGCAATCCAGGTTTAAGGTTGCATTGCCTCTTATTTTTCCTAGTCTTCACTAAATCTTGCATTTTTGGACTGACCCATTTCATCTGTAGCTCAACTGAGTTAAATTAGTCTTAAAAAGCCGGTAGATTTGTAATCTTTTATTCATACTAATACTCATCTCATGGGAAAAGATACTACCCATGTTTGAAAATTTCACATGAAAAGTTAAGCTTATAGTGGCTAAAGGTAGGACAATTGTAGGCAATTCACATTGCTGGAACGAAGGGAAAAGGGTCGACTGCGGCATTCCTTTCGAACATTTTACGAGTAGAGGGATACTCTGTTGGTTGTTATACTAGGTACAGCCTTTTTGCAACAATTATCAAATTTTCTGTATACTCTTACTCGGGATTGATTTGAGAAGGTTTAACTTTGTGATTGTGAATTTCTTCATGTGTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGAAGATCTGGAGACATGGTCTCTGGGAAGGCACTAAATTCTCTTTTCAAAAGGAATAAAGAGGTCTTTGATCAGTCAGTAGAGCTTGAAAATGGACATTTAAGTCACTTTGAGGTATTAATTCATGCTGTCTGTTGTGATTGGAAATTCTCTAGGAACTCAAATGGCTTATTTGCTGCTTCTGATTCTTCTACATGATTTTATTGAGCAAAATGATGTACCGTGTCTAGGTTTTCACTTTGTAATTTGTGTGTCACGTGATCTTTAATAATATATCCTGCATCTTTTTCTTTTTTCTTTTGGTTTCATATAGGAAGATATAAATTGTTCGTGTCAATGATAAACCTTTGAACTCTTAAAGGTCATTGGACCAATTCAGTCATTGATGTTTTCTTAATCTTTTAGACGGGCTGATGAAATTATCCCAGGTCCTTACTGCTATGGCGTTCTCGCTCTTTGCTCAAGAAGATGTTGATGTTGCAGTCATTGAGGTTCACTTTCTTTTAACTAATATTTTTCTTTGTCAAGTATTTTCTTTTATTTCCTCCAGCAAAAGCTTGCAAAAATAGCAAAAACAGAGGCTCTTGCAAAAGTTGAGGTAAGCCATTGACAGTTTGGTCTTATTTTAGGGGCTTTTGCAAAAATAGCAAAAAAAATTATGATAATAGGGCTCATATCACTACATTTTCTAAATTGTAAAAGTAACAAATTAAAAAGTTGATAACCCTATGATTACCGATATCACTAATTTTGCCATATTCGCAATATTCAAAAGATAGGCCCATAAGCTGTTTTTTTAAAAATGTTTTTGTCATTTGATGCAATTACCCCTTATTTTAACTAAAACAGGCTGGACTTGGTGGTGCACGGGATGCAACAAACATAATTTGCAGCTCTGAACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACGTGGCTGCCTTGGGTGGCTCTTTGGAAAGCATTGCAACGGCAAAGGCTGGAATAATTAAACGTGGTTGTCCAGTTAGGTTGTTTTCATGATGTAATTTTGATGCTGTTTGACTTTGTTAAGCTTTTGATATTCATTATCCACGCATTAGTTAGTTTTTCTTTTCTAATGAGTTGAAATTGTTGGTGATATATAATTAAATTTGCCGTCAACCACAAACTTAAGCTTTTGGATAAATTGGTGGTTTAATATGGTATCAGAGCAGGTGGTCCAGGGAGGTCCTGTGTTCAAGCCCACAAGTGAGAGGGAGTGTTGGTGATATATAATTAAATTTGCCTTCAACCACAAGCTTTCGAGCTTTTGGATGAATCGGTGGTTTAATTGAAATAACCTACAAGAAGTGAAGAGTTGACTAAAGTCAGCTCGAACTTGCATAAGATCACAGAAATGTTTTCTGCTTGGCTGTAATTATTGTTTGAAATAATTGTTGTGGTTGAAAGACTAAACTGAAGTTTTTGTCACAATGAACTTCAAGCACAACACAAGAAAACTAAATTGCAATCTGTCTTTCAAATTATTTTGCCATATAGAAAGGCATCAAAATTGGTCTATTGCAGTAAATGAGGCAGAAGCAACCTGAACTAAAAGGTCTTAGAGGCTTCCAAAGTCCATTGCCTTTATTCTATAGACATGATCTTGTATAGTTAGACTCCCATTCTTGTAGAAGGAGTCCGCTTTTTTTGTACGCCTATGGATTCTTTCATTTTTTTCTTAATGAAAGTTGTTCTTATTAGAAAAAAAAAGGCCCCTAATGTGATTGAATTTTCTCATATAAAGTTGTTGATGTTAGTTTATGTGTTTGTCTTTGTCTACGTGTGAGAGAGAAGTTTATTCTCCAACCCCTATTGTGATATTATTCTCCTGATTCATTCTTCCTTTTCCCTTCTGAAATTTATTCCTAAAAGTTTTCTGAATTTGATGTCAGACAATTCTGGGTGGTCCATTCCTTCCAAGAATTGAGTACATTCTTCGCGATAAAGCATTGTCCATGTCTTCGCCCGTAATATCAGCCTCAGATCCTGGAAACAGAAGTACCATAAAAGGTGTAAACCTGCTCAATGGAGGACTTAGCCAATGTTGCGACATAGTAATTCAAATAGACAATGAAGTATGGCTAGCTCATGTATTCTTAAACAAATATAATTTTACTCCTTGAACTTCAACCAAGAGTTCCTTAATCTCTTTGTCAATGCAGTTCATCGAGTTATTAGATGTCAACCTCCGCATGCTTGGACCCCATCAACTTCAGAATGCAGCAACTGCAACTTGTGTTATTCTTACTCTCCGTAATTTAGGTATAATATCATTTTGTATATTTGATTTGATATTCGTACTTTCATTTAGAGAATAAATATGTGATGTGTAATTTATTTCCGTGACACGTTTACTTAAGATGCATTTTTGAAGTGCCCTTTGCATGTGAGACCTGTGCAGAAGACAGAATACTTTAGAGACAAATGTACTAAAGTCTTAAAAAGAGATACTTAAAATTTCTTGCTTTTAGATGATATATGATGCAAGATAGATTGTACTTTATTTCACCGAATACTTTCTTTTAATATTTCCCTAATGAGAAATAATTCAGGCTGGAGAATTTCGGATGCATCTATCAGGAGTGGACTAGAGCAAACATTCCTTATTGGTAGGAGTCATTTCTTAGCAGCCAGGGAAGCTGAGGTTCTTGGACTACCTGGAGCAACGATATTGCTGGATGGAGGTGCATTCATATTTCTATCTACAATGCATTCATATTTCCGTTTACTAATGTCAAGTTTGGTCCGTTGGGACCTGATCATTGTGCTCATGCTGTGTCTTCTCTACCGTATTGTACTTAAAGATTCTTGGTAATTTGATGTTCAGTACATGGGCTCTCTTTTCACTGTCGTTGGTAAATGTTTATAATTGGTCTGCTGATGCCTGACACTTGAAGGTTTGCAGAATATTGTAGATCTGCTTAGTGTTTTCAACCTACATCAACTGATATATATTTGAGTTATGTTGTTCTGGATATTGGTGCCATGCCTTGCATTCTTTTAGACAGTGTTTTCTAATTGGTTCTTGAATGTTTTCACAGCACATACCAAAGACTCTGCTAAAGCATTATTGGATACCATTCAAATGGCTTTCCCTGAGGCTCAATTGGCTCTTGTGGTTGCAATGGCCAGTGATAAGAATCACGTTGGTTTTGCTAGAGAATTCCTTCAAGGTAAGCCTTCTCCTCTGCAAAGAGTTCGTCTCATATTTTCTTCAGCATAGAAATTTCTTCTTGATCGTAAATTCTTCGATATTCTTGAAACTTTTGGATTATATAGGCCACATCTGGTTCTTATAACCATGGTTCTTTTGAATCATATGAGAATGTCTGCAGAGATAGATTAGCTAAAGTGTGCACATTGAAATAGATGACTGGAAGAAAGTGTGTTAAGAGAAGTTGGTAAGAGAAAATATGTCTAAAGAGTGAATAAGCGAGAAAACTGTTAGCGACAGTTATGTCAAGTGAACTTATGATAGGTTCTAGAAAATTTCATTTTAGTTCTTGAATATTAGTTGAAGTTTCAAATTTTAGTTAAAAGAAGCTTAACAAACGAAAACAGAGAAAATTTTATCTTCCTTTCTTTATCATATAGGAGACTGAAAATAAGGTGTGGACTCCATCAAATTGCATGCTTCATCTTCTACCTTTGCTCTTCATTTTTTTAAACAAAAAATAAAACCTTTAATTGAAGTAATGAAAAGTAACAAATAAACTTTCACAAGGGAGAGAAAAACAAAAAGTAAAGAGAAAAAATATAAATAAAGTGAGCTTACTGAGCACCAAAATTAATACGAGTATTCTGAGAAGAAAAATCAATCAATAATTTAGGAAAATGATATCATTGATAAGCTTTTAATGTGTAAGTATTGAAGCCCTCTAGGCAACCCTGCTGTTTATTCTCTAAAGTCTTTTGATTATGGTCAATCAAATCTTAGAAATGATTGCAATTTAAACAAAGCAATAACTTTCCAAGAGATTTTATACATCTTTCCATATGTTATACAATGTCATGTAATTTTGTTCATATCTGTCTACTTTGATGATGTGAGATTGAGATGTAACAATAAAATGCTGACAGTCGGCAGTTAACTGACTTTCAGGTGGAAAATTAGAGTCCGTTGTCTTAACTGAAGCCCTTATTGGTGGAGGCAAATCAAGGACAACTTCAGCTGCTTTTCTAAGAGACTGCTGGATCCAAGCATCCAATGAATTGGGGATCCCTATTTCTTTAGAAACTAAAGATGCAGAAGTTTTCTTCACAAGCAAACTCGGAAACAGACCGGTATTGACTACTGAGACCTCGTTATTACATGCCATAAAAATTGCTGCCGAGATTCTCAAGCAGAGAACGAAAGGACGGCAAGGTCTTGTCGTAGTATCTGGCTCTCTGCATGCTGTTTCAATGGTGTTAGCTTCTCTTCATTCTTGAAGTCTTTATTTATGATAGGCACTTCTTTCGTATGTACTTTGAGCATAGTTCTTGAATATTGCCAGATTATTTTCATAGCATATTTCTATGAATTATTTGAAGTGTGACATTAATGGCAGGGAAAAGTAATGAAACAACCCTCTCAAAAGGGTGAGAAACTAGAGTAGAATACTACGGAACTCAGCATGGATGATCCAAATCTTTACAACAGCGATAAAATTTTCGATAATCTTCTTTGAACAAACTAGAATTCACTACAAGAATGCAGCAGGGAATTTTGCGACATAGGGAAGTGTTGGAAAGAAAAAAAAAAGTCAAGCGAATGAAAAAAAGTCATTGGAGATGGTTGTTGAGAAAACTTCATCAGGAGACTTTTTGCCAACGACGCTAACAAGTGTCAAGAAGAGGGTTCGGCAACCTTCAAAGGGATCAAATGCAACCTACCAGTCCAAAAATTTGGGGCATTTTGTGTTAGATTTTCACATGAAAGTATTGTGTGTGAATATTTGTGAGATACTTATTTTATTAGAATAAGAAAAAACAAATGGTTATCATGGATTCAAAAAGACAAGATTTGAGAAAGAATGGGTTTAAAAGTATAAATGCTGTTTGTGTTGATTACTCGATGGTTTCAGAAGTTGAAAGCTATTCTGAACACTATTATTGACATTCCAATTGCTGTGTACAGAGCCATTAGTGAATCATAAAGAGCAGACTGAAAAAGAGAGGTCTAATGGTGGGT

mRNA sequence

ATGAATGTCTTCAAATATCATCACCACTGTCGCCCGCAGATTCTCGGTAGCCTTCTCCTAAATCATTTCATTGGCGAATGCCCTTCTATCAGTTCCCGATTTGGGTCCAAACAATGCTTCGCCACTCACTCAGAAGACCAACACATGACCCAATTCATGGAATATTTAGATTCCCTGAAAAACTACGAGAAATTGGGTGTACCCAGAGGTTCAGGCACTGATTCTGATGATGGGTTCGATCTGGGAAGGATGAGAAGGCTGATGGAACGACTGGGTAATCCGCAATCCAGGTTTAAGGCAATTCACATTGCTGGAACGAAGGGAAAAGGGTCGACTGCGGCATTCCTTTCGAACATTTTACGAGTAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGAAGATCTGGAGACATGGTCTCTGGGAAGGCACTAAATTCTCTTTTCAAAAGGAATAAAGAGGTCTTTGATCAGTCAGTAGAGCTTGAAAATGGACATTTAAGTCACTTTGAGGTCCTTACTGCTATGGCGTTCTCGCTCTTTGCTCAAGAAGATGTTGATGTTGCAGTCATTGAGCAAAAGCTTGCAAAAATAGCAAAAACAGAGGCTCTTGCAAAAGTTGAGGCTGGACTTGGTGGTGCACGGGATGCAACAAACATAATTTGCAGCTCTGAACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACGTGGCTGCCTTGGGTGGCTCTTTGGAAAGCATTGCAACGGCAAAGGCTGGAATAATTAAACGTGGTTGTCCAACAATTCTGGGTGGTCCATTCCTTCCAAGAATTGAGTACATTCTTCGCGATAAAGCATTGTCCATGTCTTCGCCCGTAATATCAGCCTCAGATCCTGGAAACAGAAGTACCATAAAAGGTGTAAACCTGCTCAATGGAGGACTTAGCCAATGTTGCGACATAGTAATTCAAATAGACAATGAATTCATCGAGTTATTAGATGTCAACCTCCGCATGCTTGGACCCCATCAACTTCAGAATGCAGCAACTGCAACTTGTGTTATTCTTACTCTCCGTAATTTAGGCTGGAGAATTTCGGATGCATCTATCAGGAGTGGACTAGAGCAAACATTCCTTATTGGTAGGAGTCATTTCTTAGCAGCCAGGGAAGCTGAGGTTCTTGGACTACCTGGAGCAACGATATTGCTGGATGGAGCACATACCAAAGACTCTGCTAAAGCATTATTGGATACCATTCAAATGGCTTTCCCTGAGGCTCAATTGGCTCTTGTGGTTGCAATGGCCAGTGATAAGAATCACGTTGGTTTTGCTAGAGAATTCCTTCAAGGTGGAAAATTAGAGTCCGTTGTCTTAACTGAAGCCCTTATTGGTGGAGGCAAATCAAGGACAACTTCAGCTGCTTTTCTAAGAGACTGCTGGATCCAAGCATCCAATGAATTGGGGATCCCTATTTCTTTAGAAACTAAAGATGCAGAAGTTTTCTTCACAAGCAAACTCGGAAACAGACCGGTATTGACTACTGAGACCTCGTTATTACATGCCATAAAAATTGCTGCCGAGATTCTCAAGCAGAGAACGAAAGGACGGCAAGGTCTTGTCGTAGTATCTGGCTCTCTGCATGCTGTTTCAATGGGAAAAGTAATGAAACAACCCTCTCAAAAGGGTGAGAAACTAGAGTAGAATACTACGGAACTCAGCATGGATGATCCAAATCTTTACAACAGCGATAAAATTTTCGATAATCTTCTTTGAACAAACTAGAATTCACTACAAGAATGCAGCAGGGAATTTTGCGACATAGGGAAGTGTTGGAAAGAAAAAAAAAAGTCAAGCGAATGAAAAAAAGTCATTGGAGATGGTTGTTGAGAAAACTTCATCAGGAGACTTTTTGCCAACGACGCTAACAAGTGTCAAGAAGAGGGTTCGGCAACCTTCAAAGGGATCAAATGCAACCTACCAGTCCAAAAATTTGGGGCATTTTGTGTTAGATTTTCACATGAAAGTATTGTGTGTGAATATTTGTGAGATACTTATTTTATTAGAATAAGAAAAAACAAATGGTTATCATGGATTCAAAAAGACAAGATTTGAGAAAGAATGGGTTTAAAAGTATAAATGCTGTTTGTGTTGATTACTCGATGGTTTCAGAAGTTGAAAGCTATTCTGAACACTATTATTGACATTCCAATTGCTGTGTACAGAGCCATTAGTGAATCATAAAGAGCAGACTGAAAAAGAGAGGTCTAATGGTGGGT

Coding sequence (CDS)

ATGAATGTCTTCAAATATCATCACCACTGTCGCCCGCAGATTCTCGGTAGCCTTCTCCTAAATCATTTCATTGGCGAATGCCCTTCTATCAGTTCCCGATTTGGGTCCAAACAATGCTTCGCCACTCACTCAGAAGACCAACACATGACCCAATTCATGGAATATTTAGATTCCCTGAAAAACTACGAGAAATTGGGTGTACCCAGAGGTTCAGGCACTGATTCTGATGATGGGTTCGATCTGGGAAGGATGAGAAGGCTGATGGAACGACTGGGTAATCCGCAATCCAGGTTTAAGGCAATTCACATTGCTGGAACGAAGGGAAAAGGGTCGACTGCGGCATTCCTTTCGAACATTTTACGAGTAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGAAGATCTGGAGACATGGTCTCTGGGAAGGCACTAAATTCTCTTTTCAAAAGGAATAAAGAGGTCTTTGATCAGTCAGTAGAGCTTGAAAATGGACATTTAAGTCACTTTGAGGTCCTTACTGCTATGGCGTTCTCGCTCTTTGCTCAAGAAGATGTTGATGTTGCAGTCATTGAGCAAAAGCTTGCAAAAATAGCAAAAACAGAGGCTCTTGCAAAAGTTGAGGCTGGACTTGGTGGTGCACGGGATGCAACAAACATAATTTGCAGCTCTGAACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACGTGGCTGCCTTGGGTGGCTCTTTGGAAAGCATTGCAACGGCAAAGGCTGGAATAATTAAACGTGGTTGTCCAACAATTCTGGGTGGTCCATTCCTTCCAAGAATTGAGTACATTCTTCGCGATAAAGCATTGTCCATGTCTTCGCCCGTAATATCAGCCTCAGATCCTGGAAACAGAAGTACCATAAAAGGTGTAAACCTGCTCAATGGAGGACTTAGCCAATGTTGCGACATAGTAATTCAAATAGACAATGAATTCATCGAGTTATTAGATGTCAACCTCCGCATGCTTGGACCCCATCAACTTCAGAATGCAGCAACTGCAACTTGTGTTATTCTTACTCTCCGTAATTTAGGCTGGAGAATTTCGGATGCATCTATCAGGAGTGGACTAGAGCAAACATTCCTTATTGGTAGGAGTCATTTCTTAGCAGCCAGGGAAGCTGAGGTTCTTGGACTACCTGGAGCAACGATATTGCTGGATGGAGCACATACCAAAGACTCTGCTAAAGCATTATTGGATACCATTCAAATGGCTTTCCCTGAGGCTCAATTGGCTCTTGTGGTTGCAATGGCCAGTGATAAGAATCACGTTGGTTTTGCTAGAGAATTCCTTCAAGGTGGAAAATTAGAGTCCGTTGTCTTAACTGAAGCCCTTATTGGTGGAGGCAAATCAAGGACAACTTCAGCTGCTTTTCTAAGAGACTGCTGGATCCAAGCATCCAATGAATTGGGGATCCCTATTTCTTTAGAAACTAAAGATGCAGAAGTTTTCTTCACAAGCAAACTCGGAAACAGACCGGTATTGACTACTGAGACCTCGTTATTACATGCCATAAAAATTGCTGCCGAGATTCTCAAGCAGAGAACGAAAGGACGGCAAGGTCTTGTCGTAGTATCTGGCTCTCTGCATGCTGTTTCAATGGGAAAAGTAATGAAACAACCCTCTCAAAAGGGTGAGAAACTAGAGTAG

Protein sequence

MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTKGRQGLVVVSGSLHAVSMGKVMKQPSQKGEKLE*

Homology

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BLAST of CSPI03G22010 vs. ExPASy Swiss-Prot
Match: F4JYE9 (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.1e-151
Identity = 302/519 (58.19%), Postives = 376/519 (72.45%), Query Frame = 0


Query: 44  SEDQHMTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHI 103
           +ED  +  F+ +L+SLKNYEK GVP+G+GTDSDDGFDLGRM+RLM RL NP  ++K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILR  GYSVGCY+SPHI +I+ERIS   +G+ VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLG 223
           K + +QS++ ENG LSHFE+LT +AFSLF +E+VD+AVI                EAGLG
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVI----------------EAGLG 219

Query: 224 GARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPR 283
           GARDATN+I SS LAA+VIT+IGEEH+AALGGSLESIA AK+GIIK G P +LGGPFLP 
Sbjct: 220 GARDATNVIESSNLAASVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPH 279

Query: 284 IEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNG-GLSQCCDIVIQ---IDNEFIELL 343
           IE ILR KA S+SS VI AS+ G+ S+IKG+   NG GL Q CDIVIQ    D   +EL 
Sbjct: 280 IEGILRSKAASVSSSVILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELS 339

Query: 344 DVNLRMLGPHQLQNAATATCVILTLRNLG-WRISDASIRSGLEQTFLIGRSHFLAAREAE 403
           DVNLRMLG HQLQNA TATCV L LR+ G  R++D +IR GLE T L+GRS FL  +EAE
Sbjct: 340 DVNLRMLGHHQLQNAVTATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAE 399

Query: 404 VLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGK 463
            L LPGAT+LLDGAHTK+SA+AL + I+  FPE +L  VVAMASDK+HV FA+E L G K
Sbjct: 400 TLLLPGATVLLDGAHTKESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLK 459

Query: 464 LESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVL 523
            E+V+LTEA IGGGK R+T ++ L++ WI+A++ELG   S+E  +               
Sbjct: 460 PEAVILTEADIGGGKIRSTESSVLKESWIKAADELG-SRSMEASE--------------- 519

Query: 524 TTETSLLHAIKIAAEILK-QRTKGRQGLVVVSGSLHAVS 557
               ++L ++K+A +IL    T    G+V+V+GSLH VS
Sbjct: 520 --NKTVLGSLKLAYKILSDDTTSSDSGMVIVTGSLHIVS 522

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BLAST of CSPI03G22010 vs. ExPASy Swiss-Prot
Match: Q05865 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 166.4 bits (420), Expect = 9.5e-40
Identity = 122/367 (33.24%), Postives = 187/367 (50.95%), Query Frame = 0


Query: 81  LGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRE 140
           LGRM++LM RLG+P+ + +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 141 RISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQ-EDVDV 200
           RIS+  +G  +S +   +L  + K   +   + E G  + FE++TA AF  FA+   VD 
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143

Query: 201 AVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLES 260
            +                 E GLGG  D+TN++   E    VITSIG +H+  LG ++E 
Sbjct: 144 VIF----------------ETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEE 203

Query: 261 IATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNG 320
           IA  KAGIIK G P I+     P    ++R +A   ++P  S  D      I     L  
Sbjct: 204 IAGEKAGIIKEGIP-IVTAVTQPEALQVIRHEAERHAAPFQSLHD---ACVIFNEEALPA 263

Query: 321 GLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVI--LTLRNLGWRISDASIR 380
           G         + + +  E  D+   ++G HQ QNAA +      L   N+   ISD ++R
Sbjct: 264 GE----QFSFKTEEKCYE--DIRTSLIGTHQRQNAALSILAAEWLNKENIA-HISDEALR 323

Query: 381 SGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALV 440
           SGL +    GR   +              + LDGAH ++  + L +T++  F  +++++V
Sbjct: 324 SGLVKAAWPGRLELVQEH---------PPVYLDGAHNEEGVEKLAETMKQRFANSRISVV 349

Query: 441 VAMASDK 445
            +   DK
Sbjct: 384 FSALKDK 349

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BLAST of CSPI03G22010 vs. ExPASy Swiss-Prot
Match: D4GW05 (Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=folCP PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 8.4e-28
Identity = 109/365 (29.86%), Postives = 170/365 (46.58%), Query Frame = 0


Query: 84  MRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERIS 143
           +RRL+  LG+P      + +AG+ GKGSTA  +  +LR  G  VG YTSPH + +RER+ 
Sbjct: 25  IRRLLSHLGDPHEGVSFVQVAGSNGKGSTARMVDAMLRESGAHVGLYTSPHFDDVRERVR 84

Query: 144 LGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIE 203
           +   G  +   AL++     K    +    +   L+ FE +TA+A   F +  VDVAV+ 
Sbjct: 85  V--DGRKIPKSALSAFVAEAKPYLVERA-ADGEPLTFFETVTALALWYFDRAGVDVAVL- 144

Query: 204 QKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATA 263
                          E G+GG  DAT+ +   +  A+ +T++  EH A LG ++  IA  
Sbjct: 145 ---------------EVGMGGELDATSAV---DPVASAVTNVSLEHTAVLGDTVAEIAKT 204

Query: 264 KAGIIKRGCPTILG--GPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNGGL 323
           KA +     P + G  G  L     ++R++A      V++  D  +  +   V +  GG 
Sbjct: 205 KAAVAPADAPLVTGTTGEALS----VIREEA----GDVLTVGD-ADADSDADVRVSYGGR 264

Query: 324 SQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASIRSGLE 383
               +  + ++ +  E LDV + +LG +Q +NA  A  +   +R     I   +I  GL 
Sbjct: 265 VNHQEAAVTVETD-AETLDVRIPLLGAYQARNAGIAVSLARQVRP---DIDAEAIHRGLR 324

Query: 384 QTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMA 443
                GR         EV+G    T++LDGAH  D A A + T+   F    L LV    
Sbjct: 325 NAHWPGR--------FEVMGTE-PTVVLDGAHNPD-ACAQVATVLDEFDYDDLHLVYGAM 344

Query: 444 SDKNH 447
            DK+H
Sbjct: 385 HDKDH 344

[Close]

BLAST of CSPI03G22010 vs. ExPASy Swiss-Prot
Match: P15925 (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.2e-26
Identity = 100/372 (26.88%), Postives = 174/372 (46.77%), Query Frame = 0


Query: 80  DLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIR 139
           D  R+  L+  LGNPQ + + IH+ GT GKGS A  ++++L   G +VG YTSP I    
Sbjct: 21  DHRRILTLLHALGNPQQQGRYIHVTGTNGKGSAANAIAHVLEASGLTVGLYTSPFIMRFN 80

Query: 140 ERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDV 199
           ERI +     +     +N++      +     +  + +++ FE +TA+ +  F Q  VDV
Sbjct: 81  ERIMIDHE-PIPDAALVNAVAFVRAALERLQQQQADFNVTEFEFITALGYWYFRQRQVDV 140

Query: 200 AVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLES 259
           AVI                E G+GG  D+TN+I       +V+T +  +H   LG ++ +
Sbjct: 141 AVI----------------EVGIGGDTDSTNVITP---VVSVLTEVALDHQKLLGHTITA 200

Query: 260 IATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNG 319
           IA  KAGIIKRG P + G   +P    ++  K  +  S  +      +    K    L+G
Sbjct: 201 IAKHKAGIIKRGIPVVTGN-LVPDAAAVVAAKVATTGSQWLRFDRDFSVPKAK----LHG 260

Query: 320 GLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATA-TCVILTLRNLGWRISDASIRS 379
              +        +++   + D+ + ++G +Q +N A A     +  +   W ++  +IR 
Sbjct: 261 WGQR-----FTYEDQDGRISDLEVPLVGDYQQRNMAIAIQTAKVYAKQTEWPLTPQNIRQ 320

Query: 380 GLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVV 439
           GL        SH+  AR  ++   P   I++DGAH  D    L+  ++  F +  + ++ 
Sbjct: 321 GL------AASHW-PARLEKISDTP--LIVIDGAHNPDGINGLITALKQLFSQ-PITVIA 352

Query: 440 AMASDKNHVGFA 451
            + +DK++   A
Sbjct: 381 GILADKDYAAMA 352

[Close]

BLAST of CSPI03G22010 vs. ExPASy Swiss-Prot
Match: Q12676 (Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FOL3 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 6.0e-26
Identity = 107/390 (27.44%), Postives = 182/390 (46.67%), Query Frame = 0


Query: 81  LGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRE 140
           L R+ +L+E LGNPQ+  + +HIAGT GKGS   +LS++L+ + Y +G +T+PH+  + +
Sbjct: 7   LSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPHLVHVTD 66

Query: 141 RISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGH---LSHFEVLTAMAFSLFAQEDV 200
            I+       ++ K +    +R + +  Q   L   H    + FE+LT  AF  F     
Sbjct: 67  SIT-------INNKPIP--LERYQNIRLQLEALNKSHSLKCTEFELLTCTAFKYFYDVQC 126

Query: 201 DVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSL 260
              VI                E GLGG  DATN+I  +  A   IT I  +H + LG +L
Sbjct: 127 QWCVI----------------EVGLGGRLDATNVIPGANKACCGITKISLDHESFLGNTL 186

Query: 261 ESIATAKAGIIKRGCP-TILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 320
             I+  KAGII  G P T++ G     +  +++++  ++ S  +S +D    S + G N+
Sbjct: 187 SEISKEKAGIITEGVPFTVIDGTNEASVINVVKERCKALGSE-LSVTD----SQLNG-NM 246

Query: 321 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLR-NLGWRISDAS 380
           ++     C D+               L + G +Q+ N   A  ++  L+ N    I+   
Sbjct: 247 IDTNSWGCFDL-------------AKLPLNGEYQIFNLRVAMGMLDYLQMNELIDITKNE 306

Query: 381 IRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLA 440
           + + L +    GR + +  R  +V       IL+DGAH   +A  L+  ++  +    L 
Sbjct: 307 VSTRLAKVDWPGRLYRMDYRFDKVSNRT-VPILMDGAHNGSAAVELVKYLRKEYGNQPLT 349

Query: 441 LVVAMASDKNHVGFAREFLQGGKLESVVLT 466
            V+A+   KN     +  L+   ++ V+LT
Sbjct: 367 FVMAVTHGKNLEPLLQPLLR--PIDQVILT 349

[Close]

BLAST of CSPI03G22010 vs. ExPASy TrEMBL
Match: A0A0A0L7A4 (Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=3 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 3.8e-302
Identity = 541/557 (97.13%), Postives = 541/557 (97.13%), Query Frame = 0


Query: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
           RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAA 240
           FEVLTAMAFSLFAQEDVDVAVI                EAGLGGARDATNIICSSELAAA
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVI----------------EAGLGGARDATNIICSSELAAA 240

Query: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300
           VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI
Sbjct: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300

Query: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360
           SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV
Sbjct: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360

Query: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420
           ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA
Sbjct: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420

Query: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480
           LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA
Sbjct: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480

Query: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540
           FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK
Sbjct: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540

Query: 541 GRQGLVVVSGSLHAVSM 558
           GRQGLVVVSGSLHAVSM
Sbjct: 541 GRQGLVVVSGSLHAVSM 541

[Close]

BLAST of CSPI03G22010 vs. ExPASy TrEMBL
Match: A0A1S3CNR7 (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 8.9e-283
Identity = 507/557 (91.02%), Postives = 524/557 (94.08%), Query Frame = 0


Query: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSR GSKQCFATHSEDQHMT+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
           R EGYSVGCYTSPHIET+RERISLGRSG+MVSGKALNSLFKRNKEVFDQSVELE+G +SH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAA 240
           FEVLTAMAFSLFAQE+VDVAVI                EAGLGGARDATNIICSS LAAA
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVI----------------EAGLGGARDATNIICSSGLAAA 240

Query: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300
           VITSIGEEH+AALGGSLESIA AKAGIIKRGCPTILGG FLPRIEYILRDKALSMSSPVI
Sbjct: 241 VITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVI 300

Query: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360
           + SDPGNRSTIKGVN+LNGGL QCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCV
Sbjct: 301 AVSDPGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCV 360

Query: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420
           ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAA+EAEVLGLPGATILLDGAHTKDSAKA
Sbjct: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKA 420

Query: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480
           L+DTIQMAFPEA+LALV+AMASDKNHV FAREFLQGGKLE VVLTEA IGGGKSRTTSAA
Sbjct: 421 LVDTIQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAA 480

Query: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540
           FLRDCWIQAS ELGIPISLETKDAEV FTSKL NRPVLTTETSLLHAIKIAAEILKQRTK
Sbjct: 481 FLRDCWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTK 540

Query: 541 GRQGLVVVSGSLHAVSM 558
           G+Q LVVVSGSLHAVSM
Sbjct: 541 GQQSLVVVSGSLHAVSM 541

[Close]

BLAST of CSPI03G22010 vs. ExPASy TrEMBL
Match: A0A6J1CJG1 (dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011557 PE=3 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 1.1e-261
Identity = 474/557 (85.10%), Postives = 503/557 (90.31%), Query Frame = 0


Query: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
           MNVFKYH   RPQILG LLLN  +GE PSIS R GSKQCFATHSED  +T+FMEYLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
           R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALNSLFKRNKE  DQSVE ENG +SH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAA 240
           FEVLTAMAFSLFAQE+VDVAVI                EAGLGGARDATNIICSS LAAA
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVI----------------EAGLGGARDATNIICSSRLAAA 240

Query: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300
           VITSIGEEH+AALGGSLESIA AKAGIIK GCPTILGGPFLP IE IL DKALSMSS VI
Sbjct: 241 VITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVI 300

Query: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360
           SAS PG+RSTIKGV+LL+G L QCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATC 
Sbjct: 301 SASYPGHRSTIKGVSLLDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCA 360

Query: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420
           ILTLRNLGWRISD+SI++GLEQTFL+GRSHFL+A+EA VLGLPGATILLDGAHTKDSAKA
Sbjct: 361 ILTLRNLGWRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGAHTKDSAKA 420

Query: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480
           L+DTIQM FPEA+LALVVAMASDK+HVGFAREFLQGGKLES++LTEA IGGGKSRTTSAA
Sbjct: 421 LVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAA 480

Query: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540
            LRDCWIQA+NELGIPISLETKDA V  TSKL +RP+LTTETSLLHA+KIAAEILKQRT+
Sbjct: 481 ILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTE 540

Query: 541 GRQGLVVVSGSLHAVSM 558
           GR GL VV+GSLHAVSM
Sbjct: 541 GRAGLTVVTGSLHAVSM 541

[Close]

BLAST of CSPI03G22010 vs. ExPASy TrEMBL
Match: A0A6J1E819 (dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 3.3e-261
Identity = 468/557 (84.02%), Postives = 503/557 (90.31%), Query Frame = 0


Query: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
           MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF  HSEDQ MT+FMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP G+GTDS+DGFDLGRM+RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
           R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE+ DQSV+LENG +SH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAA 240
           FEVLTAMAFSLFAQE+VDVAVI                EAGLGGARDATNIICSS LAAA
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVI----------------EAGLGGARDATNIICSSRLAAA 240

Query: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300
           VITSIGEEH+AALGGSLESIA AKAGIIK GCPTILGGPF+P IE ILRDKALSMSSPV+
Sbjct: 241 VITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVV 300

Query: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360
           SASDPGNRS+IKGV+LLNG L QCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCV
Sbjct: 301 SASDPGNRSSIKGVSLLNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCV 360

Query: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420
           ILTLRNLGWRIS+ASIRSGLE+TFL+GRSHFL+A+EA +LGLPG  ILLDGAHTKDSAKA
Sbjct: 361 ILTLRNLGWRISEASIRSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDGAHTKDSAKA 420

Query: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480
           L+DTIQM+FP+AQLALVVAMASDK+H GFA EFLQGGKLES+VL+EA IGG KSRTTSAA
Sbjct: 421 LVDTIQMSFPDAQLALVVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGSKSRTTSAA 480

Query: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540
            LRDCWIQASNE+GIPISLETKDA V  TSKL NRPVLTTETSLL AIKIAAEILKQR  
Sbjct: 481 LLRDCWIQASNEMGIPISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRID 540

Query: 541 GRQGLVVVSGSLHAVSM 558
           GR+GLVVV+GSLHAVSM
Sbjct: 541 GRRGLVVVTGSLHAVSM 541

[Close]

BLAST of CSPI03G22010 vs. ExPASy TrEMBL
Match: A0A5D3BTU4 (Dihydrofolate synthetase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00160 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 4.3e-253
Identity = 461/509 (90.57%), Postives = 478/509 (93.91%), Query Frame = 0


Query: 49  MTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKG 108
           MT+FMEYLDSLKNYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKG
Sbjct: 1   MTEFMEYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKG 60

Query: 109 KGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFD 168
           KGS AAFLSNILR EGYSVGCYTSPHIET+RERISLGRSG+MVSGKALNSLFKRNKEVFD
Sbjct: 61  KGSIAAFLSNILRAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFD 120

Query: 169 QSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDA 228
           QSVELE+G +SHFEVLTAMAFSLFAQE+VDVAVI                EAGLGGARDA
Sbjct: 121 QSVELEHGRISHFEVLTAMAFSLFAQENVDVAVI----------------EAGLGGARDA 180

Query: 229 TNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYIL 288
           TNIICSS LAAAVITSIGEEH+AALGGSLESIA AKAGIIKRGCPTILGG FLPRIEYIL
Sbjct: 181 TNIICSSGLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYIL 240

Query: 289 RDKALSMSSPVISASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGP 348
           RDKALSMSSPVI+ASDPGNRSTIKGVN+LNGGL QCCDIVIQIDNEF+ELLDVNLRMLG 
Sbjct: 241 RDKALSMSSPVIAASDPGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGR 300

Query: 349 HQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATIL 408
           HQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAA+EAEVLGLPG TIL
Sbjct: 301 HQLQNAATATCVILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGVTIL 360

Query: 409 LDGAHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEAL 468
           LDGAHTKDSAKAL+DTIQMAFPEA+LALV+AMASDKNHV FAREFLQGGKLESVVLTEA 
Sbjct: 361 LDGAHTKDSAKALVDTIQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLESVVLTEAH 420

Query: 469 IGGGKSRTTSAAFLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAI 528
           IGGGKSRTTSAAFLRDCWIQAS ELGIPISLETKDAEV FTSKL NRPVLTTETSLLHAI
Sbjct: 421 IGGGKSRTTSAAFLRDCWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAI 480

Query: 529 KIAAEILKQRTKGRQGLVVVSGSLHAVSM 558
           KIAAEILKQRTKG+Q LVVVSGSLHAVSM
Sbjct: 481 KIAAEILKQRTKGQQSLVVVSGSLHAVSM 493

[Close]

BLAST of CSPI03G22010 vs. NCBI nr
Match: XP_011651415.1 (dihydrofolate synthetase isoform X1 [Cucumis sativus] >KGN57917.2 hypothetical protein Csa_010822 [Cucumis sativus])

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 556/572 (97.20%), Postives = 556/572 (97.20%), Query Frame = 0


Query: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
           RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAA 240
           FEVLTAMAFSLFAQEDVDVAVI                EAGLGGARDATNIICSSELAAA
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVI----------------EAGLGGARDATNIICSSELAAA 240

Query: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300
           VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI
Sbjct: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300

Query: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360
           SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV
Sbjct: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360

Query: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420
           ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA
Sbjct: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420

Query: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480
           LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA
Sbjct: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480

Query: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540
           FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK
Sbjct: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540

Query: 541 GRQGLVVVSGSLHAVSMGKVMKQPSQKGEKLE 573
           GRQGLVVVSGSLHAVSMGKVMKQPSQKGEKLE
Sbjct: 541 GRQGLVVVSGSLHAVSMGKVMKQPSQKGEKLE 556

[Close]

BLAST of CSPI03G22010 vs. NCBI nr
Match: XP_004149457.1 (dihydrofolate synthetase isoform X2 [Cucumis sativus])

HSP 1 Score: 1046.6 bits (2705), Expect = 7.9e-302
Identity = 541/557 (97.13%), Postives = 541/557 (97.13%), Query Frame = 0


Query: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
           RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAA 240
           FEVLTAMAFSLFAQEDVDVAVI                EAGLGGARDATNIICSSELAAA
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVI----------------EAGLGGARDATNIICSSELAAA 240

Query: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300
           VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI
Sbjct: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300

Query: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360
           SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV
Sbjct: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360

Query: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420
           ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA
Sbjct: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420

Query: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480
           LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA
Sbjct: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480

Query: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540
           FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK
Sbjct: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540

Query: 541 GRQGLVVVSGSLHAVSM 558
           GRQGLVVVSGSLHAVSM
Sbjct: 541 GRQGLVVVSGSLHAVSM 541

[Close]

BLAST of CSPI03G22010 vs. NCBI nr
Match: XP_008465388.1 (PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo])

HSP 1 Score: 982.2 bits (2538), Expect = 1.8e-282
Identity = 507/557 (91.02%), Postives = 524/557 (94.08%), Query Frame = 0


Query: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
           MNVFKYHHHCRPQILGSLLLNHFIGECPSISSR GSKQCFATHSEDQHMT+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
           R EGYSVGCYTSPHIET+RERISLGRSG+MVSGKALNSLFKRNKEVFDQSVELE+G +SH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAA 240
           FEVLTAMAFSLFAQE+VDVAVI                EAGLGGARDATNIICSS LAAA
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVI----------------EAGLGGARDATNIICSSGLAAA 240

Query: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300
           VITSIGEEH+AALGGSLESIA AKAGIIKRGCPTILGG FLPRIEYILRDKALSMSSPVI
Sbjct: 241 VITSIGEEHMAALGGSLESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVI 300

Query: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360
           + SDPGNRSTIKGVN+LNGGL QCCDIVIQIDNEF+ELLDVNLRMLG HQLQNAATATCV
Sbjct: 301 AVSDPGNRSTIKGVNMLNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCV 360

Query: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420
           ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAA+EAEVLGLPGATILLDGAHTKDSAKA
Sbjct: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDGAHTKDSAKA 420

Query: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480
           L+DTIQMAFPEA+LALV+AMASDKNHV FAREFLQGGKLE VVLTEA IGGGKSRTTSAA
Sbjct: 421 LVDTIQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTSAA 480

Query: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540
           FLRDCWIQAS ELGIPISLETKDAEV FTSKL NRPVLTTETSLLHAIKIAAEILKQRTK
Sbjct: 481 FLRDCWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQRTK 540

Query: 541 GRQGLVVVSGSLHAVSM 558
           G+Q LVVVSGSLHAVSM
Sbjct: 541 GQQSLVVVSGSLHAVSM 541

[Close]

BLAST of CSPI03G22010 vs. NCBI nr
Match: XP_038905573.1 (dihydrofolate synthetase [Benincasa hispida])

HSP 1 Score: 957.2 bits (2473), Expect = 6.3e-275
Identity = 489/557 (87.79%), Postives = 516/557 (92.64%), Query Frame = 0


Query: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
           M +FKYHHHCRPQILG+LLLNHF GECPSIS R GSKQCF+TH EDQHMT+FMEYLDSLK
Sbjct: 1   MKIFKYHHHCRPQILGTLLLNHFRGECPSISVRIGSKQCFSTHPEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP+G+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPKGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
           R EGYS GCYTSPHIETIRERISLGRSG+MVSGKALNSLFKRNKE FDQSVELENG +SH
Sbjct: 121 RAEGYSFGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180

Query: 181 FEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAA 240
           FEVLTAMAF LFAQE+VD+AVI                EAGLGGARDATNIICSS LAAA
Sbjct: 181 FEVLTAMAFLLFAQENVDLAVI----------------EAGLGGARDATNIICSSGLAAA 240

Query: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300
           VITSIGEEH+AALGGSLESIATAKAGIIKRGCP ILGGPFLP IE ILRDKALSMSSPVI
Sbjct: 241 VITSIGEEHMAALGGSLESIATAKAGIIKRGCPAILGGPFLPSIERILRDKALSMSSPVI 300

Query: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360
           SAS PGNRSTIKGV+LLNGG+ QCCD+VIQID+EFIELLDVNLRMLG HQLQNAATATCV
Sbjct: 301 SASGPGNRSTIKGVSLLNGGICQCCDVVIQIDDEFIELLDVNLRMLGHHQLQNAATATCV 360

Query: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420
           IL LRNLGWRISDAS+RSGLEQTFL+GRSHFL A+EA VLGLPGATILLDGAHTKDSAKA
Sbjct: 361 ILALRNLGWRISDASVRSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDGAHTKDSAKA 420

Query: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480
           L+DTIQMAFPEA+LALVVAMASDK+H+GFAREFLQGGKLES+VLTEA IGGGKSRTTSA 
Sbjct: 421 LVDTIQMAFPEARLALVVAMASDKDHIGFAREFLQGGKLESIVLTEAHIGGGKSRTTSAV 480

Query: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540
           FLRDCWIQASNELGIPISLETKDA V FTSKL NRPVLTTETSLLHAIK+AAEILKQRT+
Sbjct: 481 FLRDCWIQASNELGIPISLETKDAPVSFTSKLENRPVLTTETSLLHAIKVAAEILKQRTE 540

Query: 541 GRQGLVVVSGSLHAVSM 558
           GRQGLVVV+GSLHAVSM
Sbjct: 541 GRQGLVVVTGSLHAVSM 541

[Close]

BLAST of CSPI03G22010 vs. NCBI nr
Match: XP_023553540.1 (dihydrofolate synthetase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 916.0 bits (2366), Expect = 1.6e-262
Identity = 471/557 (84.56%), Postives = 506/557 (90.84%), Query Frame = 0


Query: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60
           MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF  HSEDQ MT+FMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP G+GTDS+DGFDLGRM+RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120

Query: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180
           R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE+ DQSV+LENG +SH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLGGARDATNIICSSELAAA 240
           FEVLTAMAFSLFAQE+VDVAVI                EAGLGGARDATNIICSS LAAA
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVI----------------EAGLGGARDATNIICSSRLAAA 240

Query: 241 VITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVI 300
           VITSIGEEH+AALGGSLESIA AKAGIIK GCPTILGGPF+P IE ILRDKALSMSSPV+
Sbjct: 241 VITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVV 300

Query: 301 SASDPGNRSTIKGVNLLNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCV 360
           SASDPGNRSTIKGV+LLNG L QCCD++IQ DNEFIEL DVNLRMLG HQLQNAATATCV
Sbjct: 301 SASDPGNRSTIKGVSLLNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCV 360

Query: 361 ILTLRNLGWRISDASIRSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDGAHTKDSAKA 420
           ILTLRNLGWRISDASIRSGLE+TFL+GRSHFL+A+EA +LGLPG TILLDGAHTKDSAKA
Sbjct: 361 ILTLRNLGWRISDASIRSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDGAHTKDSAKA 420

Query: 421 LLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTSAA 480
           L+DTIQM+FP+AQLALVVAMASDK+H GFA EFLQGGKLES+VL+EA IGGGKSRTTSAA
Sbjct: 421 LVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKSRTTSAA 480

Query: 481 FLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQRTK 540
            LRDCWIQASNE+GIPISLETKDA V  TSKL NRPVLTTETSLL AIKIAAEILKQR +
Sbjct: 481 LLRDCWIQASNEMGIPISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQRIE 540

Query: 541 GRQGLVVVSGSLHAVSM 558
           GR+GLVVV+GSLHAVSM
Sbjct: 541 GRRGLVVVTGSLHAVSM 541

[Close]

BLAST of CSPI03G22010 vs. TAIR 10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein )

HSP 1 Score: 537.3 bits (1383), Expect = 1.5e-152
Identity = 302/519 (58.19%), Postives = 376/519 (72.45%), Query Frame = 0


Query: 44  SEDQHMTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHI 103
           +ED  +  F+ +L+SLKNYEK GVP+G+GTDSDDGFDLGRM+RLM RL NP  ++K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILR  GYSVGCY+SPHI +I+ERIS   +G+ VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEAGLG 223
           K + +QS++ ENG LSHFE+LT +AFSLF +E+VD+AVI                EAGLG
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVI----------------EAGLG 219

Query: 224 GARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTILGGPFLPR 283
           GARDATN+I SS LAA+VIT+IGEEH+AALGGSLESIA AK+GIIK G P +LGGPFLP 
Sbjct: 220 GARDATNVIESSNLAASVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPH 279

Query: 284 IEYILRDKALSMSSPVISASDPGNRSTIKGVNLLNG-GLSQCCDIVIQ---IDNEFIELL 343
           IE ILR KA S+SS VI AS+ G+ S+IKG+   NG GL Q CDIVIQ    D   +EL 
Sbjct: 280 IEGILRSKAASVSSSVILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELS 339

Query: 344 DVNLRMLGPHQLQNAATATCVILTLRNLG-WRISDASIRSGLEQTFLIGRSHFLAAREAE 403
           DVNLRMLG HQLQNA TATCV L LR+ G  R++D +IR GLE T L+GRS FL  +EAE
Sbjct: 340 DVNLRMLGHHQLQNAVTATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAE 399

Query: 404 VLGLPGATILLDGAHTKDSAKALLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGK 463
            L LPGAT+LLDGAHTK+SA+AL + I+  FPE +L  VVAMASDK+HV FA+E L G K
Sbjct: 400 TLLLPGATVLLDGAHTKESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSGLK 459

Query: 464 LESVVLTEALIGGGKSRTTSAAFLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVL 523
            E+V+LTEA IGGGK R+T ++ L++ WI+A++ELG   S+E  +               
Sbjct: 460 PEAVILTEADIGGGKIRSTESSVLKESWIKAADELG-SRSMEASE--------------- 519

Query: 524 TTETSLLHAIKIAAEILK-QRTKGRQGLVVVSGSLHAVS 557
               ++L ++K+A +IL    T    G+V+V+GSLH VS
Sbjct: 520 --NKTVLGSLKLAYKILSDDTTSSDSGMVIVTGSLHIVS 522

[Close]

BLAST of CSPI03G22010 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 103.2 bits (256), Expect = 7.0e-22
Identity = 89/286 (31.12%), Postives = 133/286 (46.50%), Query Frame = 0


Query: 41  ATHSEDQHMTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKA 100
           AT  + +   ++   LD+L +         S   S     L    +++E L +  S+ K 
Sbjct: 2   ATEDDGELSARYQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLE-LEDAVSQMKI 61

Query: 101 IHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLF 160
           IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S +   + F
Sbjct: 62  IHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEKFVNYF 121

Query: 161 KRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEA 220
                   +    E    ++F  L  +AF +F  E VDV ++                E 
Sbjct: 122 WCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVIL----------------EV 181

Query: 221 GLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTI-LGGP 280
           GLGG  DATN+I   +     I+S+G +H+  LG +L  IA  KAGI K G P   +  P
Sbjct: 182 GLGGRFDATNVI--QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQP 241

Query: 281 FLPRIEYILRDKA--LSMSSPVISASDPGNRSTIKGVN-LLNGGLS 323
                  +L +KA  L ++  V+   D   R  ++G +  LN GL+
Sbjct: 242 --DEAMRVLNEKASKLEVNLQVVEPLDSSQRLGLQGEHQYLNAGLA 264

[Close]

BLAST of CSPI03G22010 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 101.7 bits (252), Expect = 2.0e-21
Identity = 89/286 (31.12%), Postives = 132/286 (46.15%), Query Frame = 0


Query: 41  ATHSEDQHMTQFMEYLDSLKNYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKA 100
           AT  + +   ++   LD+L +         S   S     L    +++E L +  S+ K 
Sbjct: 2   ATEDDGELSARYQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLE-LEDAVSQMKI 61

Query: 101 IHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLF 160
           IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S +   + F
Sbjct: 62  IHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEKFVNYF 121

Query: 161 KRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTEALAKVEA 220
                   +    E    ++F  L  +AF +F  E VDV ++                E 
Sbjct: 122 WCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVIL----------------EV 181

Query: 221 GLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCPTI-LGGP 280
           GLGG  DATNI    +     I+S+G +H+  LG +L  IA  KAGI K G P   +  P
Sbjct: 182 GLGGRFDATNI---QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQP 241

Query: 281 FLPRIEYILRDKA--LSMSSPVISASDPGNRSTIKGVN-LLNGGLS 323
                  +L +KA  L ++  V+   D   R  ++G +  LN GL+
Sbjct: 242 --DEAMRVLNEKASKLEVNLQVVEPLDSSQRLGLQGEHQYLNAGLA 263

[Close]

BLAST of CSPI03G22010 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 97.4 bits (241), Expect = 3.9e-20
Identity = 65/180 (36.11%), Postives = 93/180 (51.67%), Query Frame = 0


Query: 97  RFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKAL 156
           +   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L    D+   K L
Sbjct: 111 KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLD-GVDISEEKFL 170

Query: 157 NSL---FKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTE 216
                 + R KE  ++ + +     ++F  L  +AF +FA E+VD A++           
Sbjct: 171 GYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAIL----------- 230

Query: 217 ALAKVEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCP 274
                E GLGG  DATN +   +     I+S+G +H+  LG +L  IA  KAGI K G P
Sbjct: 231 -----EVGLGGKFDATNAV--QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 267

[Close]

BLAST of CSPI03G22010 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 97.4 bits (241), Expect = 3.9e-20
Identity = 65/180 (36.11%), Postives = 93/180 (51.67%), Query Frame = 0


Query: 97  RFKAIHIAGTKGKGSTAAFLSNILRVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKAL 156
           +   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L    D+   K L
Sbjct: 53  KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLD-GVDISEEKFL 112

Query: 157 NSL---FKRNKEVFDQSVELENGHLSHFEVLTAMAFSLFAQEDVDVAVIEQKLAKIAKTE 216
                 + R KE  ++ + +     ++F  L  +AF +FA E+VD A++           
Sbjct: 113 GYFWWCYNRLKERTNEEIPMP----TYFRFLALLAFKIFAAEEVDAAIL----------- 172

Query: 217 ALAKVEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGSLESIATAKAGIIKRGCP 274
                E GLGG  DATN +   +     I+S+G +H+  LG +L  IA  KAGI K G P
Sbjct: 173 -----EVGLGGKFDATNAV--QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 209

The following BLAST results are available for this feature:

BLAST of CSPI03G22010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JYE9	2.1e-151	58.19	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
Q05865	9.5e-40	33.24	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
D4GW05	8.4e-28	29.86	Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Ha...	[more]
P15925	1.2e-26	26.88	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]
Q12676	6.0e-26	27.44	Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]

BLAST of CSPI03G22010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7A4	3.8e-302	97.13	Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G35454...	[more]
A0A1S3CNR7	8.9e-283	91.02	dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3...	[more]
A0A6J1CJG1	1.1e-261	85.10	dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110115...	[more]
A0A6J1E819	3.3e-261	84.02	dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A5D3BTU4	4.3e-253	90.57	Dihydrofolate synthetase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]

BLAST of CSPI03G22010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651415.1	0.0e+00	97.20	dihydrofolate synthetase isoform X1 [Cucumis sativus] >KGN57917.2 hypothetical p...	[more]
XP_004149457.1	7.9e-302	97.13	dihydrofolate synthetase isoform X2 [Cucumis sativus]	[more]
XP_008465388.1	1.8e-282	91.02	PREDICTED: dihydrofolate synthetase isoform X1 [Cucumis melo]	[more]
XP_038905573.1	6.3e-275	87.79	dihydrofolate synthetase [Benincasa hispida]	[more]
XP_023553540.1	1.6e-262	84.56	dihydrofolate synthetase [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI03G22010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41480.1	1.5e-152	58.19	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.3	7.0e-22	31.12	DHFS-FPGS homolog D	[more]
AT3G55630.2	2.0e-21	31.12	DHFS-FPGS homolog D	[more]
AT5G05980.1	3.9e-20	36.11	DHFS-FPGS homolog B	[more]
AT5G05980.2	3.9e-20	36.11	DHFS-FPGS homolog B	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 70..385 e-value: 4.6E-80 score: 270.8
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 78..384
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 208..360 e-value: 1.3E-4 score: 22.0
IPR036615	Mur ligase, C-terminal domain superfamily	GENE3D	3.90.190.20		coord: 397..564 e-value: 4.8E-15 score: 58.1
IPR036615	Mur ligase, C-terminal domain superfamily	SUPERFAMILY	53244	MurD-like peptide ligases, peptide-binding domain	coord: 398..556
None	No IPR available	PIRSR	PIRSR038895-1	PIRSR038895-1	coord: 38..555 e-value: 1.0E-48 score: 163.9
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 46..562
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 46..562
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G22010.1	CSPI03G22010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006761	dihydrofolate biosynthetic process
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005759	mitochondrial matrix
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008841	dihydrofolate synthase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity
molecular_function	GO:0016874	ligase activity