CSPI03G21150 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G21150
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTHO complex subunit 4D
LocationChr3: 17263834 .. 17268339 (+)
RNA-Seq ExpressionCSPI03G21150
SyntenyCSPI03G21150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAATAAGGTTAAATTTCAATAACTTATGAACAAAATGGACTATTTTTGTTTACGATGTAAGAACAAAAGGTTGCAATAACAAAAGGGAAGAACTCATCTTCTTAGTCCCGAAGGAAAAGTCTCTCTCAGGTCGCCGCCGCCGCCTCCCGCCGGAAGAATCTCAGCATCTCTCTTCGACGCCCTAAAATTCCAACACCCACGATCATTTTCCATCCTTGTCCCTTCGGAATCCTCTCTTTCATCCTGTTAAACTTTCTATCTTCGCTCTCTCGGAAGTCCTTCGACTTTTTCAAGGGATAATCTTCAGGTAACTTCTTCTCATCTCTTTCTTTCCTTTTTGCAAACTCTGGTTATCATGTGAATATGTTTTATCCTTATTCTGTTAGGTTATTTTATCAATCCGAGTTTTTAATGATATTTTTGTTTTGTTTTTTGAGAAGATGCGATCGCTATTATCTCTTTTGTTATACTTGTAAGAATATGGTTGTTATTCTGTGATTGTAGATCTTAAAGTGTTTTCGAAGTTGAAATTGAGCGAGGACGTTTGGCTCTTTTGCGTAATGAATTATTGTTGTTTTTTTGCATAAAATGTTCATTAAATATCTATCGTTCTATTTGTTTGTTTGTTTGTCTTTTCTTTTTAAACTTTCTTTCTAGTATCAGTTACATTCTTGTACATATATTTTTTCCTTATAACAACTGGTGGTAGGTGTAGATATCATTTTCAATCCTTGGATGCTTATTTTCTTTTTTCTCATGCGTATATTTCCTGCTTCTGGGTTTCTGATCTGAAAACTGACTATCTTGAGTGCCTTTATTGTAATCTCATTGACTTTGGAATCAAATATATTGTATATGGGCATGGATGCTGGAGTTGGAGCAGCCATGGACGTGCGTTTTCTTTTTTAAAAACTTTTATTTAAAAAATCTGCTTACGACTGTACTTTTTTTGATCGCATGACTTCCAGAGCCTAAGGTTTTGCGAGGTCGAGGATAATCTTAAACAACATAAGAAACTATAATTACATTTGTTTTATTCTTCAACTCGTTTATCATCAAAACTATATTGTTTTCTTTTAATTATAAAATCATCACTATGGTCCACGTAAAAGAATCTTTAAGGTCATGGTGGAGTTTATTTGGGAAAGGAAAAGACCTATAAGCTTAGGATGGGGTTGTTCGACGGAATAGTTTTATTTATTTATGGTCGTTTCGAAAGCTTCAGGTGAACCAAAGTGTAACAAACTTGGGGAAGAAAAAACAGTTGTTTATTTACTTTTATAAAATCAAGTTACATAAAATGTAAATCTTTGGTTAATGCATATGTATTTGATATCGCTAATCAATAATCATACAATGAGACGAAGAAAATAATGGATAACGTACAAAAGACAAACAGTGATTGAATAATTTAATTCTTTTAACTACAAGGAGCCCAAAATATCATAAAAACAATCTTCATATTACACTTTAGAGGTCCAAGAAATAACTAAATATGACTTTTTCTTTGTTTTACTCCATTAGTGTACCCATAGTCCCCTCTACTTTAGCCTATTTTCATAGTTGTGATCTGTAAATTAAGATGAAGTTTTATTTATTGTCTTTGATAATCATTATGCTATGTAATCATTTTTTGAAAAAATAAATTCAGGCTTGTACCTCACAATGTCTTGATGTTTATGCTGGTTTGCTATTACTTTTGCCTACACTGGTGTTATGAAGCACATTAGTTGGACTGTAGTGGGGACATGAGTTTGTGTATTGTTGAAAATGGATGTTGAATAGGATGGGTGGTAGGTCATTGTCAACCAATATTGGATATATATTACAGTTGAATACATCTAATAAAGTATTGGTTTACCAAGTTTCAAGGAATTCGTTATTGTAAAAAAAAAAAATCAAGTTTCCTGCAAGTATCACGATTAATGTTTTTCTTACCATTCTGCAGGACTTCTTTTCTATAATTCCTTGAAATCTGTTTCCTCCTACCACATCGATGACTACTCCTTTGGATATGTCACTAGAGGATGTGATAAAGAAGAATAATCGTGAAAAGCTTAGAGCACGAGGTAGGGCCCGTCGTGGGCGTGGAGCAGGTGGGTCTTTTAATGGTGGAAGAGGAGTAGTGATAGGATCAGTTCGTAGAGGTCCTCTCGGCATAAATGCACGGGCATCTGCTTACTCAATTCGCAAGGCAAGCTTCAAACTGTGATGTTTAATGCATATGCTTGACAACCTGATATTCCTTGAAAATTCATGCTCATGGGGATCTCAAATAACAACACTATAGTAACTCTTAACAGAAGTGGATCAGTAAAATTGTTCTGTGGAGAATCTCATTGTGGGTCACCCTCTGATCCGAATTGTCTTCTGGTGTATTTGTACCATTATCTTTGCAAATATTGAATGCGTGTGGGTTATCTGGGTTATTTTCAAAGGACTTTCAAGGGTGGAGAATTATATTCTTGTCGATTTATAAGCTGATATGTGTTTTCCCCATCTGGATTCTTATCTGCATGCTTTCATCAGCCCCCACACAGAATGAAGAATGTTCAATGGCAGCATGATTTATTCGAAGATAGTCTTAGAGCTTCAGGGATTTCTGGAATTCAAATTGGCACAAAGTTGTACGTTTCCAACTTGGATTATGGGGTGACCAAAGAAGATATAAAGGTACAACTCAAGCCATATATATTTGACTAAAGATGCTTTCTCTTCATTTTTTTTTTTATTATTATTCACTTGGAACCTGACGGTGAACTCAGGAGTTGTTCTCTGAAATTGGAGACGTGAAAAGATTTGCAATTCATTATGACAAAAATGGTCGTCCAAGTGTAAGTTTCGCCCCTGTCTCTATAAAATTATGCAGTAATGTGTTGCTCTTTGAATTTCTTTAGAATCAAGTATCAATATTGTTTTGGTAGCCACAACTGTGTTCATTGAATCAATATTTCCTTTTGGTGGATTTTAATTTATCTGTATTCAGTACTGAATCTTTGCTTTAGAAGTTGGATAGTGCTAGAAAAAACTATTATAAGGAACTTTTGACTAGAGTACATGTAACAACTTTGAGTTAATGATAAATTATATCTATTGCATGTTTTTATATAATCTCTCTAATGGTTTACTATTCTATTTCATGAAGAAAATTACACTCCAAGTCTCCAATGTAATTAATTGATTATTAGTAACATGTGGTGCAAAAGACTCCTATAAAGTATCAATGGATTAGAATAGACGCAATCTTATTTCGAGTGAAAAAATATGTTGTATTTTTTAGTTAACTAACTTATGCCAGCTAGGCTCAACAGGTTTCATTTTTAATAAGTTCGAATGTAACTAATTTAATACGTTCAGTTCATTTTAGTCTAGATATCTCAAAACTTGTTACTCCAATCCAATCTATTAAGGTTTTCAAGGTTGAGTTTTTTTTCTCCTTTTTTAAGGACTCAACCTATTTGCAAACCAATATATAAGCTTGTGATTTCACTTTTGTATGTGGTGGTATAGTCTCATTCTTTGGTGCCAATGGTTCCTTGACCATGTATTCCCTAATTCTCGCTCGTCTTCATTGCCTTTGCTTATCATTGTTATTCATATTTGGCTTCTTTAAGTTAGCATGAGATTTGTACGAAAATATGATAGTTATCGCATTTCTATTTTAGATATGTATATAAAGGTTAGCTCCCTTATCAAGCTTTAGTTGGCTGATAAACTTCCTATTATTTATTGAAGGGCTCAGCTGAGGTGGTATATACTCGGCGAAGTGATGCATTTGCTGCTCTGAAGCGCTATAACAATGTGTTATTGGATGGGAAGCCAATGAAGATTGAAATGCTTGGTGATAATGCTGAAATGCCGGTTTCTGCACGTATAAATGTTACTGGAACAAATGGAAGAAACAGGAGGACAGTTGTTTTAACGTATGTAGTATTCTAGTTACTTTTTCTCTTTCATTTTGCCCCTTTTCGTATTTAATTTTAACATCTTTATAGGTAATTATTGTGTGCTGGAAGTAGAAAGTTTCTGCACATTATTCTATTTCATGGTCATTTTGGGGTGTTTAGGGGCCTCAACTTCAAGTTGGTGGAGTGGGTTATTATAATCCACACCATGTGTGGGGCTCCAATTATAATAGTTGGTATTTTGAACTATAACCTATAATTGTCAATATTACTTTTTCAAATCTTTTTTTACTACAATGTTTACTGTGTTTACCTTTCCAAAGGTGAGTGGTGCTGCGAGTGGGGTTAGTGCGCCTGTTGTGCTGTCTAGGTGCCTCTTCAAAGAGGTGATTGACCAGCGACACCTTGTTGCACCTTCGTTGATGAAGAAGGCTAGTGCCTCTTCCAATATCTGTGTTTTCTTTCTTTTTTTATTTAATTGATCAACAACCCATGAAATCGATTACTCAACCTTTTGAATTTGGCCGCTTCCTTCTACTTCGCTTTGGTTTCTTCCTTTTAAACTCTTTGATATCATATTCTTTCCTGAATGACAACC

mRNA sequence

GGAAAATAAGGTTAAATTTCAATAACTTATGAACAAAATGGACTATTTTTGTTTACGATGTAAGAACAAAAGGTTGCAATAACAAAAGGGAAGAACTCATCTTCTTAGTCCCGAAGGAAAAGTCTCTCTCAGGTCGCCGCCGCCGCCTCCCGCCGGAAGAATCTCAGCATCTCTCTTCGACGCCCTAAAATTCCAACACCCACGATCATTTTCCATCCTTGTCCCTTCGGAATCCTCTCTTTCATCCTGTTAAACTTTCTATCTTCGCTCTCTCGGAAGTCCTTCGACTTTTTCAAGGGATAATCTTCAGGACTTCTTTTCTATAATTCCTTGAAATCTGTTTCCTCCTACCACATCGATGACTACTCCTTTGGATATGTCACTAGAGGATGTGATAAAGAAGAATAATCGTGAAAAGCTTAGAGCACGAGGTAGGGCCCGTCGTGGGCGTGGAGCAGGTGGGTCTTTTAATGGTGGAAGAGGAGTAGTGATAGGATCAGTTCGTAGAGGTCCTCTCGGCATAAATGCACGGGCATCTGCTTACTCAATTCGCAAGCCCCCACACAGAATGAAGAATGTTCAATGGCAGCATGATTTATTCGAAGATAGTCTTAGAGCTTCAGGGATTTCTGGAATTCAAATTGGCACAAAGTTGTACGTTTCCAACTTGGATTATGGGGTGACCAAAGAAGATATAAAGGAGTTGTTCTCTGAAATTGGAGACGTGAAAAGATTTGCAATTCATTATGACAAAAATGGTCGTCCAAGTGGCTCAGCTGAGGTGGTATATACTCGGCGAAGTGATGCATTTGCTGCTCTGAAGCGCTATAACAATGTGTTATTGGATGGGAAGCCAATGAAGATTGAAATGCTTGGTGATAATGCTGAAATGCCGGTTTCTGCACGTATAAATGTTACTGGAACAAATGGAAGAAACAGGAGGACAGTTGTTTTAACGTGAGTGGTGCTGCGAGTGGGGTTAGTGCGCCTGTTGTGCTGTCTAGGTGCCTCTTCAAAGAGGTGATTGACCAGCGACACCTTGTTGCACCTTCGTTGATGAAGAAGGCTAGTGCCTCTTCCAATATCTGTGTTTTCTTTCTTTTTTTATTTAATTGATCAACAACCCATGAAATCGATTACTCAACCTTTTGAATTTGGCCGCTTCCTTCTACTTCGCTTTGGTTTCTTCCTTTTAAACTCTTTGATATCATATTCTTTCCTGAATGACAACC

Coding sequence (CDS)

ATGACTACTCCTTTGGATATGTCACTAGAGGATGTGATAAAGAAGAATAATCGTGAAAAGCTTAGAGCACGAGGTAGGGCCCGTCGTGGGCGTGGAGCAGGTGGGTCTTTTAATGGTGGAAGAGGAGTAGTGATAGGATCAGTTCGTAGAGGTCCTCTCGGCATAAATGCACGGGCATCTGCTTACTCAATTCGCAAGCCCCCACACAGAATGAAGAATGTTCAATGGCAGCATGATTTATTCGAAGATAGTCTTAGAGCTTCAGGGATTTCTGGAATTCAAATTGGCACAAAGTTGTACGTTTCCAACTTGGATTATGGGGTGACCAAAGAAGATATAAAGGAGTTGTTCTCTGAAATTGGAGACGTGAAAAGATTTGCAATTCATTATGACAAAAATGGTCGTCCAAGTGGCTCAGCTGAGGTGGTATATACTCGGCGAAGTGATGCATTTGCTGCTCTGAAGCGCTATAACAATGTGTTATTGGATGGGAAGCCAATGAAGATTGAAATGCTTGGTGATAATGCTGAAATGCCGGTTTCTGCACGTATAAATGTTACTGGAACAAATGGAAGAAACAGGAGGACAGTTGTTTTAACGTGA

Protein sequence

MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARASAYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPVSARINVTGTNGRNRRTVVLT*
Homology
BLAST of CSPI03G21150 vs. ExPASy Swiss-Prot
Match: Q6NQ72 (THO complex subunit 4D OS=Arabidopsis thaliana OX=3702 GN=ALY4 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.3e-60
Identity = 121/204 (59.31%), Postives = 161/204 (78.92%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRAR-RGRGAGGSFNGGRGVVIGSVRRGPLGINARA 60
           M+  L+M+L++++K+    +   RG +R RGRG GG   GGRG   G  RRGPL +NAR 
Sbjct: 1   MSGALNMTLDEIVKRGKTARSGGRGISRGRGRGRGG---GGRGA--GPARRGPLAVNARP 60

Query: 61  SAYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSE 120
           S+++I KP  R++++ WQ  LFED LRA+G SG+++GT+L+V+NLD GVT EDI+ELFSE
Sbjct: 61  SSFTINKPVRRVRSLPWQSGLFEDGLRAAGASGVEVGTRLHVTNLDQGVTNEDIRELFSE 120

Query: 121 IGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN--AE 180
           IG+V+R+AIHYDKNGRPSG+AEVVY RRSDAF ALK+YNNVLLDG+PM++E+LG N  +E
Sbjct: 121 IGEVERYAIHYDKNGRPSGTAEVVYPRRSDAFQALKKYNNVLLDGRPMRLEILGGNNSSE 180

Query: 181 MPVSAR--INVTGTNGRNRRTVVL 200
            P+S R  +NVTG NGR +RTVV+
Sbjct: 181 APLSGRVNVNVTGLNGRLKRTVVI 199

BLAST of CSPI03G21150 vs. ExPASy Swiss-Prot
Match: Q94EH8 (THO complex subunit 4C OS=Arabidopsis thaliana OX=3702 GN=ALY3 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 5.7e-56
Identity = 116/212 (54.72%), Postives = 163/212 (76.89%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRA-----RGRARR-GRGAGGS---FNGGRGVVIGSVRRG 60
           M+  L+M+L++++KK+  E+  A     +G +R+ GRG GG      GGRG   G VRRG
Sbjct: 1   MSDALNMTLDEIVKKSKSERSAAARSGGKGVSRKSGRGRGGPNGVVGGGRGG--GPVRRG 60

Query: 61  PLGINAR-ASAYSIRKPPHRMKNVQW--QHDLFEDSLRASGISGIQIGTKLYVSNLDYGV 120
           PL +N R +S++SI K   R +++ W  Q+DL+E++LRA G+SG+++GT +Y++NLD GV
Sbjct: 61  PLAVNTRPSSSFSINKLARRKRSLPWQNQNDLYEETLRAVGVSGVEVGTTVYITNLDQGV 120

Query: 121 TKEDIKELFSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMK 180
           T EDI+EL++EIG++KR+AIHYDKNGRPSGSAEVVY RRSDA  A+++YNNVLLDG+PMK
Sbjct: 121 TNEDIRELYAEIGELKRYAIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDGRPMK 180

Query: 181 IEMLGDNAE-MPVSARINVTGTNGRNRRTVVL 200
           +E+LG N E  PV+AR+NVTG NGR +R+V +
Sbjct: 181 LEILGGNTESAPVAARVNVTGLNGRMKRSVFI 210

BLAST of CSPI03G21150 vs. ExPASy Swiss-Prot
Match: Q8L719 (THO complex subunit 4B OS=Arabidopsis thaliana OX=3702 GN=ALY2 PE=1 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.7e-39
Identity = 94/191 (49.21%), Postives = 123/191 (64.40%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           M+  LDMSL+D+I K+NR+   +RGR   G G      GG G   G  RR    + AR +
Sbjct: 1   MSGGLDMSLDDII-KSNRKPTGSRGRGGIGGGNNTGGRGGSGSNSGPSRRFANRVGARTA 60

Query: 61  AYSIRKPPHRMKNVQWQHDLF--EDSLRAS----------GISGIQIGTKLYVSNLDYGV 120
            YS      +  +  WQ+D+F  + S+ A+          G S I+ GTKLY+SNLDYGV
Sbjct: 61  PYSRPIQQQQAHDAMWQNDVFATDASVAAAFGHHQTAVVGGGSSIETGTKLYISNLDYGV 120

Query: 121 TKEDIKELFSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMK 180
           + EDIKELFSE+GD+KR+ IHYD++GR  G+AEVV++RR DA AA+KRYNNV LDGK MK
Sbjct: 121 SNEDIKELFSEVGDLKRYGIHYDRSGRSKGTAEVVFSRRGDALAAVKRYNNVQLDGKLMK 180

BLAST of CSPI03G21150 vs. ExPASy Swiss-Prot
Match: Q8L773 (THO complex subunit 4A OS=Arabidopsis thaliana OX=3702 GN=ALY1 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.5e-37
Identity = 86/181 (47.51%), Postives = 116/181 (64.09%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           M+T LDMSL+D+I KN     ++RG A   RG G     G      + R  P   + R++
Sbjct: 1   MSTGLDMSLDDMIAKNR----KSRGGAGPARGTGSGSGPG-----PTRRNNPNRKSTRSA 60

Query: 61  AYSIRKPPHRMKNVQWQHDLF----EDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKEL 120
            Y   K P       W HD+F    ED       +GI+ GTKLY+SNLDYGV  EDIKEL
Sbjct: 61  PYQSAKAPES----TWGHDMFSDRSEDHRSGRSSAGIETGTKLYISNLDYGVMNEDIKEL 120

Query: 121 FSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNA 178
           F+E+G++KR+ +H+D++GR  G+AEVVY+RR DA AA+K+YN+V LDGKPMKIE++G N 
Sbjct: 121 FAEVGELKRYTVHFDRSGRSKGTAEVVYSRRGDALAAVKKYNDVQLDGKPMKIEIVGTNL 168

BLAST of CSPI03G21150 vs. ExPASy Swiss-Prot
Match: B5FXN8 (THO complex subunit 4 OS=Taeniopygia guttata OX=59729 GN=ALYREF PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-30
Identity = 78/181 (43.09%), Postives = 114/181 (62.98%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGG----RGVVIGSVRRGPL--- 60
           M   +DMSL+D+IK N  ++  +RG  R GRG GG+  GG     GV  G    GP+   
Sbjct: 1   MADKMDMSLDDIIKLNRSQRGASRG-GRGGRGRGGTARGGGPGRGGVGGGRAGGGPVRNR 60

Query: 61  GINARASAYSIRKPPHRMKNV--QWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKE 120
            + AR    +   P  R K +  +WQHDLF+    A   +G++ G KL VSNLD+GV+  
Sbjct: 61  PVMARGGGRNRPAPYSRPKQLPEKWQHDLFDSGFGAG--AGVETGGKLLVSNLDFGVSDA 120

Query: 121 DIKELFSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEM 173
           DI+ELF+E G +K+ A+HYD++GR  G+A+V + R++DA  A+K+YN V LDG+PM I++
Sbjct: 121 DIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQL 178

BLAST of CSPI03G21150 vs. ExPASy TrEMBL
Match: A0A0A0L717 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G302100 PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 2.8e-106
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS
Sbjct: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60

Query: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120
           AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI
Sbjct: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120

Query: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180
           GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV
Sbjct: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180

Query: 181 SARINVTGTNGRNRRTVVLT 201
           SARINVTGTNGRNRRTVVLT
Sbjct: 181 SARINVTGTNGRNRRTVVLT 200

BLAST of CSPI03G21150 vs. ExPASy TrEMBL
Match: A0A1S3C452 (THO complex subunit 4D OS=Cucumis melo OX=3656 GN=LOC103496802 PE=4 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 1.4e-105
Identity = 198/200 (99.00%), Postives = 200/200 (100.00%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS
Sbjct: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60

Query: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120
           AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDI+ELFSEI
Sbjct: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180
           GD+KRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180

Query: 181 SARINVTGTNGRNRRTVVLT 201
           SARINVTGTNGRNRRTVVLT
Sbjct: 181 SARINVTGTNGRNRRTVVLT 200

BLAST of CSPI03G21150 vs. ExPASy TrEMBL
Match: A0A5D3BPA9 (THO complex subunit 4D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G002100 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 4.6e-101
Identity = 198/228 (86.84%), Postives = 200/228 (87.72%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS
Sbjct: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60

Query: 61  AYSIRK----------------------------PPHRMKNVQWQHDLFEDSLRASGISG 120
           AYSIRK                            PPHRMKNVQWQHDLFEDSLRASGISG
Sbjct: 61  AYSIRKASFKLSGSVMLFCGESHCGSPSDPNCLLPPHRMKNVQWQHDLFEDSLRASGISG 120

Query: 121 IQIGTKLYVSNLDYGVTKEDIKELFSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFA 180
           IQIGTKLYVSNLDYGVTKEDI+ELFSEIGD+KRFAIHYDKNGRPSGSAEVVYTRRSDAFA
Sbjct: 121 IQIGTKLYVSNLDYGVTKEDIRELFSEIGDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFA 180

Query: 181 ALKRYNNVLLDGKPMKIEMLGDNAEMPVSARINVTGTNGRNRRTVVLT 201
           ALKRYNNVLLDGKPMKIEMLGDNAEMPVSARINVTGTNGRNRRTVVLT
Sbjct: 181 ALKRYNNVLLDGKPMKIEMLGDNAEMPVSARINVTGTNGRNRRTVVLT 228

BLAST of CSPI03G21150 vs. ExPASy TrEMBL
Match: A0A6J1J564 (THO complex subunit 4D-like OS=Cucurbita maxima OX=3661 GN=LOC111481464 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 9.6e-99
Identity = 186/200 (93.00%), Postives = 193/200 (96.50%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           M TPLDMSLEDVIKKNNREKLRARGRARRGRGAGGS+NGGR VVIGSVRRGPLGINAR S
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120
           A+SI KPP RMKNVQWQHDLFEDSLRASGISGI+IGTKLYVSNLDYGVTKEDI+ELFSEI
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180
           GD+KRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNA+ PV
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNADTPV 180

Query: 181 SARINVTGTNGRNRRTVVLT 201
           SARINVTG NGR+RRTVVLT
Sbjct: 181 SARINVTGVNGRSRRTVVLT 200

BLAST of CSPI03G21150 vs. ExPASy TrEMBL
Match: A0A6J1FAB9 (THO complex subunit 4D-like OS=Cucurbita moschata OX=3662 GN=LOC111442248 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 9.6e-99
Identity = 186/200 (93.00%), Postives = 193/200 (96.50%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           M TPLDMSLEDVIKKNNREKLRARGRARRGRGAGGS+NGGR VVIGSVRRGPLGINAR S
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120
           A+SI KPP RMKNVQWQHDLFEDSLRASGISGI+IGTKLYVSNLDYGVTKEDI+ELFSEI
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180
           GD+KRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNA+ PV
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNADTPV 180

Query: 181 SARINVTGTNGRNRRTVVLT 201
           SARINVTG NGR+RRTVVLT
Sbjct: 181 SARINVTGVNGRSRRTVVLT 200

BLAST of CSPI03G21150 vs. NCBI nr
Match: XP_004145851.1 (THO complex subunit 4D [Cucumis sativus] >KAE8650594.1 hypothetical protein Csa_011773 [Cucumis sativus])

HSP 1 Score: 394.4 bits (1012), Expect = 5.7e-106
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS
Sbjct: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60

Query: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120
           AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI
Sbjct: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120

Query: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180
           GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV
Sbjct: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180

Query: 181 SARINVTGTNGRNRRTVVLT 201
           SARINVTGTNGRNRRTVVLT
Sbjct: 181 SARINVTGTNGRNRRTVVLT 200

BLAST of CSPI03G21150 vs. NCBI nr
Match: XP_008457020.1 (PREDICTED: THO complex subunit 4D [Cucumis melo])

HSP 1 Score: 392.1 bits (1006), Expect = 2.9e-105
Identity = 198/200 (99.00%), Postives = 200/200 (100.00%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS
Sbjct: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60

Query: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120
           AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDI+ELFSEI
Sbjct: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180
           GD+KRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180

Query: 181 SARINVTGTNGRNRRTVVLT 201
           SARINVTGTNGRNRRTVVLT
Sbjct: 181 SARINVTGTNGRNRRTVVLT 200

BLAST of CSPI03G21150 vs. NCBI nr
Match: TYK01611.1 (THO complex subunit 4D [Cucumis melo var. makuwa])

HSP 1 Score: 377.1 bits (967), Expect = 9.5e-101
Identity = 198/228 (86.84%), Postives = 200/228 (87.72%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS
Sbjct: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60

Query: 61  AYSIRK----------------------------PPHRMKNVQWQHDLFEDSLRASGISG 120
           AYSIRK                            PPHRMKNVQWQHDLFEDSLRASGISG
Sbjct: 61  AYSIRKASFKLSGSVMLFCGESHCGSPSDPNCLLPPHRMKNVQWQHDLFEDSLRASGISG 120

Query: 121 IQIGTKLYVSNLDYGVTKEDIKELFSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFA 180
           IQIGTKLYVSNLDYGVTKEDI+ELFSEIGD+KRFAIHYDKNGRPSGSAEVVYTRRSDAFA
Sbjct: 121 IQIGTKLYVSNLDYGVTKEDIRELFSEIGDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFA 180

Query: 181 ALKRYNNVLLDGKPMKIEMLGDNAEMPVSARINVTGTNGRNRRTVVLT 201
           ALKRYNNVLLDGKPMKIEMLGDNAEMPVSARINVTGTNGRNRRTVVLT
Sbjct: 181 ALKRYNNVLLDGKPMKIEMLGDNAEMPVSARINVTGTNGRNRRTVVLT 228

BLAST of CSPI03G21150 vs. NCBI nr
Match: XP_038896761.1 (THO complex subunit 4D-like [Benincasa hispida])

HSP 1 Score: 377.1 bits (967), Expect = 9.5e-101
Identity = 189/200 (94.50%), Postives = 197/200 (98.50%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           M TPLDMSLEDVIKK+NREKLRARGRARRGRGAGGSFNGGRGVV+GSVRRGPLGINARAS
Sbjct: 1   MATPLDMSLEDVIKKSNREKLRARGRARRGRGAGGSFNGGRGVVVGSVRRGPLGINARAS 60

Query: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120
           AYSIRKPP RMKNVQWQHDLFEDSLRASGISGI+IGTKLYVSNLDYGV+KEDI+ELFSEI
Sbjct: 61  AYSIRKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVSKEDIRELFSEI 120

Query: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180
           GD+KRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDG+PMKIEMLGDNAEMPV
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGRPMKIEMLGDNAEMPV 180

Query: 181 SARINVTGTNGRNRRTVVLT 201
           SARINVTG NGR+RRTVVLT
Sbjct: 181 SARINVTGVNGRSRRTVVLT 200

BLAST of CSPI03G21150 vs. NCBI nr
Match: XP_022935328.1 (THO complex subunit 4D-like [Cucurbita moschata] >XP_022935329.1 THO complex subunit 4D-like [Cucurbita moschata] >XP_022935330.1 THO complex subunit 4D-like [Cucurbita moschata])

HSP 1 Score: 369.4 bits (947), Expect = 2.0e-98
Identity = 186/200 (93.00%), Postives = 193/200 (96.50%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           M TPLDMSLEDVIKKNNREKLRARGRARRGRGAGGS+NGGR VVIGSVRRGPLGINAR S
Sbjct: 1   MATPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSYNGGRAVVIGSVRRGPLGINARPS 60

Query: 61  AYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSEI 120
           A+SI KPP RMKNVQWQHDLFEDSLRASGISGI+IGTKLYVSNLDYGVTKEDI+ELFSEI
Sbjct: 61  AFSISKPPRRMKNVQWQHDLFEDSLRASGISGIEIGTKLYVSNLDYGVTKEDIRELFSEI 120

Query: 121 GDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNAEMPV 180
           GD+KRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNA+ PV
Sbjct: 121 GDLKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDNADTPV 180

Query: 181 SARINVTGTNGRNRRTVVLT 201
           SARINVTG NGR+RRTVVLT
Sbjct: 181 SARINVTGVNGRSRRTVVLT 200

BLAST of CSPI03G21150 vs. TAIR 10
Match: AT5G37720.1 (ALWAYS EARLY 4 )

HSP 1 Score: 234.2 bits (596), Expect = 9.3e-62
Identity = 121/204 (59.31%), Postives = 161/204 (78.92%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRAR-RGRGAGGSFNGGRGVVIGSVRRGPLGINARA 60
           M+  L+M+L++++K+    +   RG +R RGRG GG   GGRG   G  RRGPL +NAR 
Sbjct: 1   MSGALNMTLDEIVKRGKTARSGGRGISRGRGRGRGG---GGRGA--GPARRGPLAVNARP 60

Query: 61  SAYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSE 120
           S+++I KP  R++++ WQ  LFED LRA+G SG+++GT+L+V+NLD GVT EDI+ELFSE
Sbjct: 61  SSFTINKPVRRVRSLPWQSGLFEDGLRAAGASGVEVGTRLHVTNLDQGVTNEDIRELFSE 120

Query: 121 IGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN--AE 180
           IG+V+R+AIHYDKNGRPSG+AEVVY RRSDAF ALK+YNNVLLDG+PM++E+LG N  +E
Sbjct: 121 IGEVERYAIHYDKNGRPSGTAEVVYPRRSDAFQALKKYNNVLLDGRPMRLEILGGNNSSE 180

Query: 181 MPVSAR--INVTGTNGRNRRTVVL 200
            P+S R  +NVTG NGR +RTVV+
Sbjct: 181 APLSGRVNVNVTGLNGRLKRTVVI 199

BLAST of CSPI03G21150 vs. TAIR 10
Match: AT5G37720.2 (ALWAYS EARLY 4 )

HSP 1 Score: 234.2 bits (596), Expect = 9.3e-62
Identity = 121/204 (59.31%), Postives = 161/204 (78.92%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRAR-RGRGAGGSFNGGRGVVIGSVRRGPLGINARA 60
           M+  L+M+L++++K+    +   RG +R RGRG GG   GGRG   G  RRGPL +NAR 
Sbjct: 1   MSGALNMTLDEIVKRGKTARSGGRGISRGRGRGRGG---GGRGA--GPARRGPLAVNARP 60

Query: 61  SAYSIRKPPHRMKNVQWQHDLFEDSLRASGISGIQIGTKLYVSNLDYGVTKEDIKELFSE 120
           S+++I KP  R++++ WQ  LFED LRA+G SG+++GT+L+V+NLD GVT EDI+ELFSE
Sbjct: 61  SSFTINKPVRRVRSLPWQSGLFEDGLRAAGASGVEVGTRLHVTNLDQGVTNEDIRELFSE 120

Query: 121 IGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMKIEMLGDN--AE 180
           IG+V+R+AIHYDKNGRPSG+AEVVY RRSDAF ALK+YNNVLLDG+PM++E+LG N  +E
Sbjct: 121 IGEVERYAIHYDKNGRPSGTAEVVYPRRSDAFQALKKYNNVLLDGRPMRLEILGGNNSSE 180

Query: 181 MPVSAR--INVTGTNGRNRRTVVL 200
            P+S R  +NVTG NGR +RTVV+
Sbjct: 181 APLSGRVNVNVTGLNGRLKRTVVI 199

BLAST of CSPI03G21150 vs. TAIR 10
Match: AT1G66260.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 218.8 bits (556), Expect = 4.0e-57
Identity = 116/212 (54.72%), Postives = 163/212 (76.89%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRA-----RGRARR-GRGAGGS---FNGGRGVVIGSVRRG 60
           M+  L+M+L++++KK+  E+  A     +G +R+ GRG GG      GGRG   G VRRG
Sbjct: 1   MSDALNMTLDEIVKKSKSERSAAARSGGKGVSRKSGRGRGGPNGVVGGGRGG--GPVRRG 60

Query: 61  PLGINAR-ASAYSIRKPPHRMKNVQW--QHDLFEDSLRASGISGIQIGTKLYVSNLDYGV 120
           PL +N R +S++SI K   R +++ W  Q+DL+E++LRA G+SG+++GT +Y++NLD GV
Sbjct: 61  PLAVNTRPSSSFSINKLARRKRSLPWQNQNDLYEETLRAVGVSGVEVGTTVYITNLDQGV 120

Query: 121 TKEDIKELFSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMK 180
           T EDI+EL++EIG++KR+AIHYDKNGRPSGSAEVVY RRSDA  A+++YNNVLLDG+PMK
Sbjct: 121 TNEDIRELYAEIGELKRYAIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDGRPMK 180

Query: 181 IEMLGDNAE-MPVSARINVTGTNGRNRRTVVL 200
           +E+LG N E  PV+AR+NVTG NGR +R+V +
Sbjct: 181 LEILGGNTESAPVAARVNVTGLNGRMKRSVFI 210

BLAST of CSPI03G21150 vs. TAIR 10
Match: AT1G66260.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 218.8 bits (556), Expect = 4.0e-57
Identity = 116/212 (54.72%), Postives = 163/212 (76.89%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRA-----RGRARR-GRGAGGS---FNGGRGVVIGSVRRG 60
           M+  L+M+L++++KK+  E+  A     +G +R+ GRG GG      GGRG   G VRRG
Sbjct: 1   MSDALNMTLDEIVKKSKSERSAAARSGGKGVSRKSGRGRGGPNGVVGGGRGG--GPVRRG 60

Query: 61  PLGINAR-ASAYSIRKPPHRMKNVQW--QHDLFEDSLRASGISGIQIGTKLYVSNLDYGV 120
           PL +N R +S++SI K   R +++ W  Q+DL+E++LRA G+SG+++GT +Y++NLD GV
Sbjct: 61  PLAVNTRPSSSFSINKLARRKRSLPWQNQNDLYEETLRAVGVSGVEVGTTVYITNLDQGV 120

Query: 121 TKEDIKELFSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMK 180
           T EDI+EL++EIG++KR+AIHYDKNGRPSGSAEVVY RRSDA  A+++YNNVLLDG+PMK
Sbjct: 121 TNEDIRELYAEIGELKRYAIHYDKNGRPSGSAEVVYMRRSDAIQAMRKYNNVLLDGRPMK 180

Query: 181 IEMLGDNAE-MPVSARINVTGTNGRNRRTVVL 200
           +E+LG N E  PV+AR+NVTG NGR +R+V +
Sbjct: 181 LEILGGNTESAPVAARVNVTGLNGRMKRSVFI 210

BLAST of CSPI03G21150 vs. TAIR 10
Match: AT5G02530.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 164.1 bits (414), Expect = 1.2e-40
Identity = 94/191 (49.21%), Postives = 123/191 (64.40%), Query Frame = 0

Query: 1   MTTPLDMSLEDVIKKNNREKLRARGRARRGRGAGGSFNGGRGVVIGSVRRGPLGINARAS 60
           M+  LDMSL+D+I K+NR+   +RGR   G G      GG G   G  RR    + AR +
Sbjct: 1   MSGGLDMSLDDII-KSNRKPTGSRGRGGIGGGNNTGGRGGSGSNSGPSRRFANRVGARTA 60

Query: 61  AYSIRKPPHRMKNVQWQHDLF--EDSLRAS----------GISGIQIGTKLYVSNLDYGV 120
            YS      +  +  WQ+D+F  + S+ A+          G S I+ GTKLY+SNLDYGV
Sbjct: 61  PYSRPIQQQQAHDAMWQNDVFATDASVAAAFGHHQTAVVGGGSSIETGTKLYISNLDYGV 120

Query: 121 TKEDIKELFSEIGDVKRFAIHYDKNGRPSGSAEVVYTRRSDAFAALKRYNNVLLDGKPMK 180
           + EDIKELFSE+GD+KR+ IHYD++GR  G+AEVV++RR DA AA+KRYNNV LDGK MK
Sbjct: 121 SNEDIKELFSEVGDLKRYGIHYDRSGRSKGTAEVVFSRRGDALAAVKRYNNVQLDGKLMK 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NQ721.3e-6059.31THO complex subunit 4D OS=Arabidopsis thaliana OX=3702 GN=ALY4 PE=1 SV=1[more]
Q94EH85.7e-5654.72THO complex subunit 4C OS=Arabidopsis thaliana OX=3702 GN=ALY3 PE=1 SV=1[more]
Q8L7191.7e-3949.21THO complex subunit 4B OS=Arabidopsis thaliana OX=3702 GN=ALY2 PE=1 SV=1[more]
Q8L7734.5e-3747.51THO complex subunit 4A OS=Arabidopsis thaliana OX=3702 GN=ALY1 PE=1 SV=1[more]
B5FXN81.1e-3043.09THO complex subunit 4 OS=Taeniopygia guttata OX=59729 GN=ALYREF PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L7172.8e-106100.00RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G302100 PE=4 SV... [more]
A0A1S3C4521.4e-10599.00THO complex subunit 4D OS=Cucumis melo OX=3656 GN=LOC103496802 PE=4 SV=1[more]
A0A5D3BPA94.6e-10186.84THO complex subunit 4D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4... [more]
A0A6J1J5649.6e-9993.00THO complex subunit 4D-like OS=Cucurbita maxima OX=3661 GN=LOC111481464 PE=4 SV=... [more]
A0A6J1FAB99.6e-9993.00THO complex subunit 4D-like OS=Cucurbita moschata OX=3662 GN=LOC111442248 PE=4 S... [more]
Match NameE-valueIdentityDescription
XP_004145851.15.7e-106100.00THO complex subunit 4D [Cucumis sativus] >KAE8650594.1 hypothetical protein Csa_... [more]
XP_008457020.12.9e-10599.00PREDICTED: THO complex subunit 4D [Cucumis melo][more]
TYK01611.19.5e-10186.84THO complex subunit 4D [Cucumis melo var. makuwa][more]
XP_038896761.19.5e-10194.50THO complex subunit 4D-like [Benincasa hispida][more]
XP_022935328.12.0e-9893.00THO complex subunit 4D-like [Cucurbita moschata] >XP_022935329.1 THO complex sub... [more]
Match NameE-valueIdentityDescription
AT5G37720.19.3e-6259.31ALWAYS EARLY 4 [more]
AT5G37720.29.3e-6259.31ALWAYS EARLY 4 [more]
AT1G66260.14.0e-5754.72RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT1G66260.24.0e-5754.72RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT5G02530.11.2e-4049.21RNA-binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 98..170
e-value: 2.6E-19
score: 80.2
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 99..167
e-value: 7.0E-16
score: 57.8
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 97..174
score: 15.391531
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 94..173
e-value: 1.9E-22
score: 81.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..35
NoneNo IPR availablePANTHERPTHR19965RNA AND EXPORT FACTOR BINDING PROTEINcoord: 1..200
NoneNo IPR availablePANTHERPTHR19965:SF33THO COMPLEX SUBUNIT 4Ccoord: 1..200
NoneNo IPR availableCDDcd12680RRM_THOC4coord: 97..171
e-value: 3.6361E-43
score: 137.386
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 47..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G21150.1CSPI03G21150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding