CSPI03G20930 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G20930
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Valyl-tRNA synthetase
Location	Chr3: 16976534 .. 16983212 (-)
RNA-Seq Expression	CSPI03G20930
Synteny	CSPI03G20930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGCATTGACAAAACTCTAAATTAATGACCGAAGAAATGAAAGGGCTAGGGGATACGTGAGAGATCAGAGGTTGGTGAAATGGTGGAAAAGAGTATGGAAGAAGATATTAGCTCCATGGAGGGTTTCACCTAACGATTTAAGACGGAGATGAGCAATTTGGAGAAGCTCATTCGGAGACGGAAAAAAGAGAGTACACAGAAGAAAAAAGGAAGATGAGCGATTTGGAGAAGGAAAACATGAAGAAAGTAGAAGGAAGGAAAGAGAGGGAGTGAGAAACATTGCTAGAGGAGACACGAAAAAAGTAGAGGGAACGGAGAAAGGAGGAGTGAGGAAGAGTGACAGAGGGGAAAGAGAAGTGAGGAACTGTAACAAAGAAGAAAGTGGGAAACAATGACATAAGAGAAAGAAGGAGTGAGGAAGAAGATGAGAGCCAGAGATAGATTTGAAAAACCCTAATCTTAGTATTATGATAAAATACTTGACTCCACTTTGTTGAATATGAGGGGACAGACAACCCCTTAGGAATAAGAGCATGTGTTTTAAGTCTTATTCTTGTAATATCTAATTGATGGTCTATGGCTGGATGTGCTAGAAGGCCCAACAAACCATAGATGTTAATCAGGTCCGAAATTATCTGTACAAACCCCCCTCCAACCTAGTGGGTAAATTTTGGAATTCTTACAAAAAGAAAAAAAAAGAAAAAGAAAAAGAAGGAACTCTTTGGTCAGATAAAATAGTCGTCGTCGCTCACATAGCTCAATCCCATTCACTGGCACTGAAGAGCCTCAGCCATTCCATTAAACCCCAAACCCCTCTACAATTCAATTCCTTCACTTCCATTTCACTCCCACCATTTCTGAGCACAACAAACCTTTCGTCAAAACATGCTTCTTCAAATGGCCTTCTCTGGCCCTTACTTCCTCCATCCTTCCTCTGCTTCCAGACTCAATCCCCTTCTCTTTACCAAGCACCGGCGTTCTTTTGGCCTCTCCCGCTTCCGATTCCGTCGTTTACACCAGAGACAATTAGCTGGTACTCTCACTTCTCCTCGTGTTTGGTTGCTTTTGTGGGTAGTGCCAATGGGTTTCTCTCCCTTTTTTTTTTTTGCCCGATTTCTCATGATTTTTTTTCCTATGGATTTTAGTGGCTGAATTTTAACTCATTATTTGCAAGAGATATTTTCTGAAGTCTCGTTATAACTAAATTTACTATAAACCATTAGCTTAAGCTTTCCGGGTGATGTAACATGGTATAATAGCATTTGTTTTAATTCTATTAATATTGATTTCAATCTGTTGTATCTTCTACAAATTTCATGGCCGCAAGTAAAGGAATTGTTGATTAAATTTACCATATTTAAGCCTTTGGGTCAATCAATGAAATCTATGGGTTTAAGGTTCATCAATGTTATAAAAAGCTATTGTGGCTTATGTCTTGAGCCAAAACTTCAACTTTTAGCCTCAAAGCTTACACTTTCCTTGAATTACAAAAGGCATACGTCCTTGTAAAAACACTCCAAGGCTTAAAATCTAAAATAATATTGATTTGACTCATTCTTAAAAATATAAAGGTTTAAGCAGTGACGATTTGAACGATTACTTCTTTTACTTTTGTGACTCTAAGAACATTGAAAAAGAAATTTATAGTATAAATGAATTCTATTAATCATAGTAGTAATGATGTTAAATACGTCCTTTCAGAATTTTTTGTTAAGAGAAAATTGTGGGTTTTTTTCCCCACTTTTATATGAATATGGCGTTTGGCATTGATTGATCATACTAATTTAGAATTTTTAAAATGTTTTTTATTCAATTTGAGGCTTACGCCTCATGTTGCCTTAAGGCTTACTCCTCGTCTTGTCAAGAGGAAAATTCTCAAGGCCACCTTTAGCCTTTTATTGCATAGAGGTTCATTGAGAATTTCATTCTGTTGTTTGCAATTGGTGTTTTGGTGTTCATGAACTTTGTCCGAGTACCATACGTTGGAAACGAAACAAGTTTTGAATAATTTGTTTTGCTTGAGAACCGTGATTAAGCATTTGACAGAGATAGAAAGGACAACTTTCTTTCGGGCTGGATTCGTGGTGGTCATGGATATAATAATTTATAATTAGTAGGAGTGACCTCTTAATCTTGATTCGAACAAGGGCTAGAATTAGACTCGATAGACAAAGTATAACATTCATTTGCATGTGTGTATATATATTCACCAGTAGTTCATGGAATTTTCAGTTGCTGCATCAGCGAATGGTGTTTTTACTTCTCCAGAGATAGCAAAGACGTTTGACTTTGCTTCGGAAGAGCGAATATACAAATGGTAATCAATATCATGTCAAATATCTCACAGTATTTAACATTAAGATCACTTCTCTTTTTGTTGACATTGGCGTGTTGCTTTAGCCTATTTGATGGCAAATTTTTGTCTTGTGCTACTTCATGGATTTGTATTTTATCTAATTGTGTTGAAAAGAAGTTAGTGCACTATTTCAATAACAGATTGCTCGTTAAAGATTCCAAGAATGTACTGGTATTTCTTTTTCTGCAATTGAGCTGGCTTCTTCTTAACATTTTATTTAATTTTTTAAACTCAGATCAATGATAAATGTAAGGAGAATTTTAGGAAATAGTCAGAAATGCAGACTTAGTTATTCCTTAGTAGAGTTTCTACATCTTTGTTCCATTAGATATGGTTCTACTATTTCACTTTATTTTATTTATTATTTTTTTTAATGTTCTAAGTAAACAACATATTCTCGTTTGTTCCATTGATTTAAATGACCAAAATTGAATGTACCTCTTTTATTTTATTTTATTTTTTGGCAAGGTAAATGAGTGCCTAATCTTCATTACTGAGTTTCACTTGATCGTGAGAAGAAAAAACAAATTTGCTTACTTACTTATGGTTATTAAATGCTCTCTTTGTCTCGTTAGGTGGGAATCTCAAGGTTATTTTAGGCCACATCATGATCAAGATACTGTTCCGTTTGTGATTTCAATGCCACCTCCCAATGTGACAGGATCATTGCACATGGGACATGCGATGTTTGTGACTCTTGAGGTTGTGAAACTAAGAGCTAGCTGGTAGCCCGTATTTGATCATGAATTTTCTTTCTGCAACCAACTCTACATGATAATACAATTTATTGTATTAAAGTATTGATATAAGATTTAAATGACCTTAATCCATTAGTTTAAACTTTTGCGTCTATGGGGGTTATGGTATCCAAATAGGAGGCTAATGTAGCTTAAATAGCCTCAAGGAGCAATCCTTCAAGAATCAAACTTGTGAGTCTTTTCTTAGAATGAGCTTCATGTAGGGGGGAAATTCATATTTATAAAGTTTATTATAACTGGGGGGTAAAAGTGAATTATCCTTGAATAACTCTATTCTTTCTACATCAAGATAAAGATTATATGGCAAACAACCTAGTTTTTCTTATTGTAGCCCATTATTTTTATCATCACTTTTTACCTAGTAGTTTATGCCCAATATCCGATGCTACCAGAAAATTACAGTGAAATAATATTCCCAGTGACAAAGACTTAGACTGTTTAGTTGTCTTATGGTCCTCAGTCAATTATTTTGAGCAGGCCTTCATTTTTTTTCCTTGTTATCTTTGCTCTACTTGTCTCTCAAAAATTGCTATTGGTTTCCCTTGCCAGGATATTATGGTTAGATACAACCGTATGAAGGGAAGACCAACACTCTGGCTTCCTGGGACTGATCATGCAGGTATTGCCACACAGGTTTGTCTCTAAAGCGAGCCAGTAGACTGCTGTCTTTATTTCATTCGCTAGTATCTCATAAACACATAAAACAAGACAAAATATTCAAATAATTTCACTCTTCAATGCTGGAGGAATTTCTCCTTATTTTTGTATGGATGGCTGTCTAAGTCAGTGATGGGATATATTTCATTTTCCTATTTTCTACTTTTTTTATTTAAGTTTAATCTAGAGGCATATTCTCTTGCATTTCCTTGCAGTTGGTTGTTGAAAGAATGCTTGCGTCTGAAGGAATAAAAAGGGTTGAATTGGGTAGAGATGAATTTACAAAGCGAGTTTGGGAGTGGAAAGAGAAGTATACAATCTCACACAATTTGCTTTGTTCGTTATTTTTTTTGGTATTGAAGTTTGCTTGCTTCTATGAAAAATAATAATGGTAATTTGTAACCTTGCATATCAAGATAATTCATAGCTTTGTAATTTTTTAAAATTTCATCTTAGCATATTATGGTCCTAAATCTTGTATCTCCATTTTCTGCTTCTTTGTATTCTAATGATAACTTTTCTGGTGAAGTTGCTGAAGTTCCGTTTTTAGGGTAAGAAAAAATGGGTGTGGCTTCATGTTCTTGAATAAGAACAACTATCCTGTGTTATTCCTAATCCTACCATTCATTGTTGATCCAAAGCAAGCATTTCACAATATTTATGATATGAATCTTCATTGTTTGCATATCTATTCTAGCAGCTATCTCATTTAAAATGAATGATTTTTTTCCCATTCAAACTTTAAAGGTATGGTGGAACTATCACTAATCAAATTAAGAGACTTGGAGCTTCCTGTGATTGGACTAAAGAGCACTTTACACTTGATGATCAGCTAAGTCGTAAGAGAGTTTTCCATTGCATTTATTTAGTAGGCACTAGACATAAAAGAGGGTTAATGATTGCATGACTGTAAGTTATGTCAGCTGAAATGTTATCTAACAAATTGCAATTGAGTGGTGTCTCATTCTTGCACTGATATTTGCCATACTTAGGTGCCTTTTATGCTATTTTGAGTTCTTTAGATTGAAAGTTTATATTTTTTTTCTCCTTTGACTCCTTATGGGTGTTTGTATGCATAGACGTTGAACTTTTAACTCATCATGGAAAATAATTTTATTTAAAGAAAAAGATTATTAAATTAAAAATTTGGTCCCGAGTGTAATTTCAATTTGGTCCTTGAAATTTCAATTTAGCTCCTAAAATTGAGCTTAATTTCAATTTGATTCTGGTGGTTTTAAAGGTTCTATTTGGTTTTGTGATTTGGTTAAACCTCACACATTGTCCCTAATTAGACTCTTTTCCACCCGAAAATTCACTTATTTCATTCTAAGAAAAGCTTTTCTTTTGCCAAAATTTTATTTTGAGTATAATGTCTATAAAAATCAAGTCAATTGAAATGCATTTTCTATGTCCATCTCTTTTGTCTAATTCTTTTTTTTTTTTAATTGGATGATCTCTTAGAAAAATAAATAAATTTTGTTACAAGTCCATTTAACTTACTTTCTTACCATGGTCAGTTGGCTTGAAAGCAATTTCCACACTTGCTGATACACACTTTTGTATATTTAGGAGCCGTTATTGAGGCATTTGTTCGACTTCATGAAAGAGGCTTGATCTATCAAGGTAGAATACTGATTTCTGAAGGGCCTCTTTGTACTATGTTTCATATAATGCAGAAGATGATGATTCATCCAGTCCCAAATTTGGTCTTTTTTAATGTATTAATTGTTCTTTGTGATATTGGGCTTTATTTTTGTCTTGGTCTTGAACTTTTGCTTGTTCTTCCATTTTGATTCCGAAGCTTTTGAAACCTTGATTTTGGTAGCTGAACTTTTATCTATATTCCATTTTGATCCCTGGGTTAAGTCATTAAATTTTCATTGAATTCCAATGCCATATATAAAATTTTACCATTATGGTAAAGCTTAGCTGTGGACCCTTACACCTGCCTCACTTTCTGTTAGATCAGACTGTTAAACTTGAGAGTCTGAGACAAGCTCTTCTAATTGTGTTATTAGGTAATTTAACCTATTCTTAGTACAATGGTTCCAGTATCTGTTAGCCATTACCTCAGTTTTAAGCTTAAAATTTACTCAGTGTTCCCTAGTTAACGCCACTTATTTCCATTGATTACAGCCTGTTTAATGGGCAGTGAATCATATCATAAAGGTTTTAGAATTGTTACTTTATAGAAGAATTTGGAATAATGAAGGGGGAACCTAAACCATATCTGAGATTTGATTGGTTGTGTTTTTATCTTCTTGGTATCTTAAATCTAAATTCTTTTTGTAATTTATCTGTCTACACTATCTCGAACAAGTGGATATCTTTATTTAATTGTTTGGCTGACTCTATTATGTGTTCCATATATAAAAAAAACAAAAAACAAAAATACCAAGAAGAAAAAAAAAAAAACTGGAATAAAGAACTCGTGTGTTCTTCAATTTTTTGCCATTGTTGGAGGTGCCATTCTCAAACTATTATGCCATAAAACCAGTATGGGAGCAATTAAAGGATAAATTCATCTTCTGATAGAAATATTTCATTTACATGATATTTTAGTTTTTTGTAAAAGAGATATTCAGATTAATGAGTTCCTTTATTAAAATTGAAAATCATGTACGAATTTCTCTCAAGTTAAAGTCGTCTAAATAACTAGCTGATTACTGGATTTATTTATTTATTTCCTCAGGGTCTTACATGGTAAACTGGTCTCCGAATTTGCAAACTGCTGTTTCTGACCTGGTAGGTCTCTAGTTTATTGGCAGAATTAGTTCTTTTATTACTTTTTGTTCTCAATTTTTACTTTCTAACGTGAATGGTAACTTTGCCTAGGAAGTAGAGTACTCTGAAGAATCTGGTACTCTCTATCATATTAAGTATCGAGTGGCTGGAGGTTCAAGGTAG

mRNA sequence

ATTGCATTGACAAAACTCTAAATTAATGACCGAAGAAATGAAAGGGCTAGGGGATACGTGAGAGATCAGAGGTTGGTGAAATGGTGGAAAAGAGTATGGAAGAAGATATTAGCTCCATGGAGGGTTTCACCTAACGATTTAAGACGGAGATGAGCAATTTGGAGAAGCTCATTCGGAGACGGAAAAAAGAGAGTACACAGAAGAAAAAAGGAAGATGAGCGATTTGGAGAAGGAAAACATGAAGAAAGTAGAAGGAAGGAAAGAGAGGGAGTGAGAAACATTGCTAGAGGAGACACGAAAAAAGTAGAGGGAACGGAGAAAGGAGGAGTGAGGAAGAGTGACAGAGGGGAAAGAGAAGTGAGGAACTGTAACAAAGAAGAAAGTGGGAAACAATGACATAAGAGAAAGAAGGAGTGAGGAAGAAGATGAGAGCCAGAGATAGATTTGAAAAACCCTAATCTTAGTATTATGATAAAATACTTGACTCCACTTTGTTGAATATGAGGGGACAGACAACCCCTTAGGAATAAGAGCATGTGTTTTAAGTCTTATTCTTGTAATATCTAATTGATGGTCTATGGCTGGATGTGCTAGAAGGCCCAACAAACCATAGATGTTAATCAGGTCCGAAATTATCTGTACAAACCCCCCTCCAACCTAGTGGGTAAATTTTGGAATTCTTACAAAAAGAAAAAAAAAGAAAAAGAAAAAGAAGGAACTCTTTGGTCAGATAAAATAGTCGTCGTCGCTCACATAGCTCAATCCCATTCACTGGCACTGAAGAGCCTCAGCCATTCCATTAAACCCCAAACCCCTCTACAATTCAATTCCTTCACTTCCATTTCACTCCCACCATTTCTGAGCACAACAAACCTTTCGTCAAAACATGCTTCTTCAAATGGCCTTCTCTGGCCCTTACTTCCTCCATCCTTCCTCTGCTTCCAGACTCAATCCCCTTCTCTTTACCAAGCACCGGCGTTCTTTTGGCCTCTCCCGCTTCCGATTCCGTCGTTTACACCAGAGACAATTAGCTGTTGCTGCATCAGCGAATGGTGTTTTTACTTCTCCAGAGATAGCAAAGACGTTTGACTTTGCTTCGGAAGAGCGAATATACAAATGGTGGGAATCTCAAGGTTATTTTAGGCCACATCATGATCAAGATACTGTTCCGTTTGTGATTTCAATGCCACCTCCCAATGTGACAGGATCATTGCACATGGGACATGCGATGTTTGTGACTCTTGAGGATATTATGGTTAGATACAACCGTATGAAGGGAAGACCAACACTCTGGCTTCCTGGGACTGATCATGCAGGTATTGCCACACAGTTGGTTGTTGAAAGAATGCTTGCGTCTGAAGGAATAAAAAGGGTTGAATTGGGTAGAGATGAATTTACAAAGCGAGTTTGGGAGTGGAAAGAGAAGTATGGTGGAACTATCACTAATCAAATTAAGAGACTTGGAGCTTCCTGTGATTGGACTAAAGAGCACTTTACACTTGATGATCAGCTAAGTCGAGCCGTTATTGAGGCATTTGTTCGACTTCATGAAAGAGGCTTGATCTATCAAGGGTCTTACATGGTAAACTGGTCTCCGAATTTGCAAACTGCTGTTTCTGACCTGGAAGTAGAGTACTCTGAAGAATCTGGTACTCTCTATCATATTAAGTATCGAGTGGCTGGAGGTTCAAGGTAG

Coding sequence (CDS)

ATGCTTCTTCAAATGGCCTTCTCTGGCCCTTACTTCCTCCATCCTTCCTCTGCTTCCAGACTCAATCCCCTTCTCTTTACCAAGCACCGGCGTTCTTTTGGCCTCTCCCGCTTCCGATTCCGTCGTTTACACCAGAGACAATTAGCTGTTGCTGCATCAGCGAATGGTGTTTTTACTTCTCCAGAGATAGCAAAGACGTTTGACTTTGCTTCGGAAGAGCGAATATACAAATGGTGGGAATCTCAAGGTTATTTTAGGCCACATCATGATCAAGATACTGTTCCGTTTGTGATTTCAATGCCACCTCCCAATGTGACAGGATCATTGCACATGGGACATGCGATGTTTGTGACTCTTGAGGATATTATGGTTAGATACAACCGTATGAAGGGAAGACCAACACTCTGGCTTCCTGGGACTGATCATGCAGGTATTGCCACACAGTTGGTTGTTGAAAGAATGCTTGCGTCTGAAGGAATAAAAAGGGTTGAATTGGGTAGAGATGAATTTACAAAGCGAGTTTGGGAGTGGAAAGAGAAGTATGGTGGAACTATCACTAATCAAATTAAGAGACTTGGAGCTTCCTGTGATTGGACTAAAGAGCACTTTACACTTGATGATCAGCTAAGTCGAGCCGTTATTGAGGCATTTGTTCGACTTCATGAAAGAGGCTTGATCTATCAAGGGTCTTACATGGTAAACTGGTCTCCGAATTTGCAAACTGCTGTTTCTGACCTGGAAGTAGAGTACTCTGAAGAATCTGGTACTCTCTATCATATTAAGTATCGAGTGGCTGGAGGTTCAAGGTAG

Protein sequence

MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTSPEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEKYGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQTAVSDLEVEYSEESGTLYHIKYRVAGGSR*

Homology

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BLAST of CSPI03G20930 vs. ExPASy Swiss-Prot
Match: F4KE63 (Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2247 PE=3 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 4.1e-118
Identity = 209/273 (76.56%), Postives = 232/273 (84.98%), Query Frame = 0


Query: 1   MLLQMAFSGP----YFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQ---LAVAAS 60
           M+L+ AFS P      L PSS  +LN L FT+ RR   +S  R   +  ++    + AAS
Sbjct: 1   MILKTAFSLPTPTTTLLSPSSPHQLNTLFFTRRRRRL-ISPSRLNSIFSQRRFSFSAAAS 60

Query: 61  ANGVFTSPEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGH 120
            N VFTSPE +KTFDF+SEE+IYKWWESQGYF+P+ DQ   PFVI MPPPNVTGSLHMGH
Sbjct: 61  GNNVFTSPETSKTFDFSSEEKIYKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHMGH 120

Query: 121 AMFVTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKR 180
           AMFVTLEDIMVRYNRM GRPTLWLPGTDHAGIATQLVVE+MLASEGIKRV+LGRDEFTKR
Sbjct: 121 AMFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVDLGRDEFTKR 180

Query: 181 VWEWKEKYGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMV 240
           VWEWKEKYGGTITNQIKRLGASCDW++E FTLD+QLSRAV+EAFV+LH++GLIYQGSYMV
Sbjct: 181 VWEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMV 240

Query: 241 NWSPNLQTAVSDLEVEYSEESGTLYHIKYRVAG 267
           NWSPNLQTAVSDLEVEYSEE G LYHIKYRVAG
Sbjct: 241 NWSPNLQTAVSDLEVEYSEEPGFLYHIKYRVAG 272

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BLAST of CSPI03G20930 vs. ExPASy Swiss-Prot
Match: C1D6J9 (Valine--tRNA ligase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=valS PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 9.3e-70
Identity = 123/206 (59.71%), Postives = 154/206 (74.76%), Query Frame = 0


Query: 62  EIAKTFDFAS-EERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 121
           E+AK+F+  + E R Y  WE+ GYF+P  D D   F I +PPPNVTG+LHMGHA   T+ 
Sbjct: 2   ELAKSFEPGNIENRWYDRWEAAGYFKPSMDTDRPSFCIQLPPPNVTGTLHMGHAFNQTIM 61

Query: 122 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 181
           D + RY RMKG  TLW+PG DHAGIATQ+VVER LA +G+ R +LGRD F  +VWEWKEK
Sbjct: 62  DGLTRYYRMKGDNTLWVPGADHAGIATQIVVERQLAEQGVSRHDLGRDTFIGKVWEWKEK 121

Query: 182 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 241
            GGTIT+Q++R+G S DW KE+FT+DD++S AV E FVRL+E+GLIY+G  +VNW P L 
Sbjct: 122 SGGTITSQMRRVGCSVDWDKEYFTMDDKMSTAVTEVFVRLYEQGLIYRGKRLVNWDPKLG 181

Query: 242 TAVSDLEVEYSEESGTLYHIKYRVAG 267
           TAVSDLEV   EE G ++HI+Y V G
Sbjct: 182 TAVSDLEVISEEEDGKMWHIRYPVVG 207

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BLAST of CSPI03G20930 vs. ExPASy Swiss-Prot
Match: Q6F8F0 (Valine--tRNA ligase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=valS PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.9e-67
Identity = 121/205 (59.02%), Postives = 152/205 (74.15%), Query Frame = 0


Query: 59  TSPEIAKTFDFAS-EERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFV 118
           ++  IA T+D    E + Y+ WE +GYF+P    D+  F I +PPPNVTGSLHMGH    
Sbjct: 12  SAQNIATTYDPTDIERKWYQIWEEKGYFKPSGQGDS--FCIMIPPPNVTGSLHMGHGFNN 71

Query: 119 TLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEW 178
            + D + RYNRM G+ TLW PGTDHAGIATQ+VVER LA++ I R +LGR++F  +VWEW
Sbjct: 72  AIMDALTRYNRMMGKNTLWQPGTDHAGIATQMVVERQLAAQNISRHDLGREQFIDKVWEW 131

Query: 179 KEKYGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSP 238
           KE+ GGTIT QI+RLG+S DW++E FT+DD LS AV E FV+LHE GLIY+G  +VNW P
Sbjct: 132 KEQSGGTITKQIRRLGSSVDWSRERFTMDDGLSNAVKEVFVKLHEDGLIYRGKRLVNWDP 191

Query: 239 NLQTAVSDLEVEYSEESGTLYHIKY 263
            LQTA+SDLEVE  EE G+L+H KY
Sbjct: 192 KLQTALSDLEVESVEEKGSLWHFKY 214

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BLAST of CSPI03G20930 vs. ExPASy Swiss-Prot
Match: Q82X51 (Valine--tRNA ligase OS=Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298) OX=228410 GN=valS PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.5e-67
Identity = 123/211 (58.29%), Postives = 154/211 (72.99%), Query Frame = 0


Query: 62  EIAKTFDFAS-EERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 121
           E+AK+FD    E R Y  WE+ GYF P   +   P+ I +PPPNVTG+LHMGHA   TL 
Sbjct: 2   ELAKSFDPKEIETRWYSTWETAGYFSPTDHKGADPYCIMLPPPNVTGTLHMGHAFQHTLM 61

Query: 122 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 181
           D ++RY+RM G  TLW PGTDHAGIATQ+VVER L  EG  R ++GR+ F +RVW+WKE+
Sbjct: 62  DALIRYHRMLGDNTLWQPGTDHAGIATQIVVERQLDQEGKDRRQMGREAFLERVWQWKEE 121

Query: 182 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 241
            G TIT Q++R+GASCDW++E FT+D+ LSRAV E FVRL+  GLIY+G  +VNW P LQ
Sbjct: 122 SGSTITRQMRRMGASCDWSRERFTMDETLSRAVTEVFVRLYREGLIYRGKRLVNWDPVLQ 181

Query: 242 TAVSDLEVEYSEESGTLYHIKY---RVAGGS 269
           TAVSDLEV   EE G+L+HI Y   ++ GGS
Sbjct: 182 TAVSDLEVVSVEEQGSLWHILYPFEQLEGGS 212

[Close]

BLAST of CSPI03G20930 vs. ExPASy Swiss-Prot
Match: Q0VSA8 (Valine--tRNA ligase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2) OX=393595 GN=valS PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.3e-66
Identity = 119/197 (60.41%), Postives = 146/197 (74.11%), Query Frame = 0


Query: 72  EERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLEDIMVRYNRMKG 131
           E+  Y+ WE  G+F+P    D  PF I +PPPNVTGSLHMGHA   T+ D +VRY RM+G
Sbjct: 13  EQSWYENWEQAGHFKPSGQGD--PFCIMIPPPNVTGSLHMGHAFQDTIMDTLVRYRRMQG 72

Query: 132 RPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEKYGGTITNQIKR 191
           R TLW  GTDHAGIATQ+VVER LA EG  R ELGR++F  +VWEWK + GGTIT Q++R
Sbjct: 73  RNTLWQVGTDHAGIATQMVVERKLAGEGTNRHELGREKFLDKVWEWKRESGGTITRQLRR 132

Query: 192 LGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQTAVSDLEVEYS 251
           +GAS DWT+E FT+DD LS AV E FVRLH+ GLIY+G  +VNW P L TA+SDLEVE  
Sbjct: 133 MGASVDWTRERFTMDDGLSNAVREVFVRLHKEGLIYRGKRLVNWDPALHTAISDLEVENV 192

Query: 252 EESGTLYHIKYRVAGGS 269
           EE G ++H +Y ++ GS
Sbjct: 193 EEQGHMWHFRYPLSDGS 207

[Close]

BLAST of CSPI03G20930 vs. ExPASy TrEMBL
Match: A0A0A0LCI4 (tRNA-synt_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G285540 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.5e-155
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS
Sbjct: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSR 270
           TAVSDLEVEYSEESGTLYHIKYRVAGGSR
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSR 269

[Close]

BLAST of CSPI03G20930 vs. ExPASy TrEMBL
Match: A0A1S3C571 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.0e-151
Identity = 264/268 (98.51%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLL MAFSGP FLHPSSASRLNPLLFTKHRRSF LSRFRFRRLHQRQLAVAASANGVFTS
Sbjct: 1   MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIYKWWESQGYFRPH+DQDTVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 269
           TAVSDLEVEYSEESGTLYHIKYRVAGGS
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 268

[Close]

BLAST of CSPI03G20930 vs. ExPASy TrEMBL
Match: A0A1S3C576 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.0e-151
Identity = 264/268 (98.51%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLL MAFSGP FLHPSSASRLNPLLFTKHRRSF LSRFRFRRLHQRQLAVAASANGVFTS
Sbjct: 1   MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIYKWWESQGYFRPH+DQDTVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 269
           TAVSDLEVEYSEESGTLYHIKYRVAGGS
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 268

[Close]

BLAST of CSPI03G20930 vs. ExPASy TrEMBL
Match: A0A1S3C4H7 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.0e-151
Identity = 264/268 (98.51%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLL MAFSGP FLHPSSASRLNPLLFTKHRRSF LSRFRFRRLHQRQLAVAASANGVFTS
Sbjct: 1   MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIYKWWESQGYFRPH+DQDTVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 269
           TAVSDLEVEYSEESGTLYHIKYRVAGGS
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 268

[Close]

BLAST of CSPI03G20930 vs. ExPASy TrEMBL
Match: A0A6J1IZ88 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.5e-147
Identity = 257/269 (95.54%), Postives = 262/269 (97.40%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLLQMAFSGP  LHPSSA RLNPL+FTK RRSFGLSRF FRRL QRQLAVAASANGVFTS
Sbjct: 1   MLLQMAFSGPSLLHPSSAYRLNPLIFTKCRRSFGLSRFHFRRLQQRQLAVAASANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIY+WWESQGYFRPH DQD+VPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYRWWESQGYFRPHFDQDSVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAV+EAFVRLHERGLIYQGSYMVNWSP+LQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVVEAFVRLHERGLIYQGSYMVNWSPHLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSR 270
           TAVSDLEVEYSEESGTLYHIKYRVAGGSR
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSR 269

[Close]

BLAST of CSPI03G20930 vs. NCBI nr
Match: XP_011651387.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis sativus] >KAE8650589.1 hypothetical protein Csa_011094 [Cucumis sativus])

HSP 1 Score: 556.6 bits (1433), Expect = 1.2e-154
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS
Sbjct: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 269
           TAVSDLEVEYSEESGTLYHIKYRVAGGS
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 268

[Close]

BLAST of CSPI03G20930 vs. NCBI nr
Match: XP_008457007.1 (PREDICTED: valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucumis melo])

HSP 1 Score: 545.8 bits (1405), Expect = 2.1e-151
Identity = 264/268 (98.51%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLL MAFSGP FLHPSSASRLNPLLFTKHRRSF LSRFRFRRLHQRQLAVAASANGVFTS
Sbjct: 1   MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIYKWWESQGYFRPH+DQDTVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 269
           TAVSDLEVEYSEESGTLYHIKYRVAGGS
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 268

[Close]

BLAST of CSPI03G20930 vs. NCBI nr
Match: XP_008457012.1 (PREDICTED: valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X6 [Cucumis melo])

HSP 1 Score: 545.8 bits (1405), Expect = 2.1e-151
Identity = 264/268 (98.51%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLL MAFSGP FLHPSSASRLNPLLFTKHRRSF LSRFRFRRLHQRQLAVAASANGVFTS
Sbjct: 1   MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIYKWWESQGYFRPH+DQDTVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 269
           TAVSDLEVEYSEESGTLYHIKYRVAGGS
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 268

[Close]

BLAST of CSPI03G20930 vs. NCBI nr
Match: XP_008457006.1 (PREDICTED: valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis melo])

HSP 1 Score: 545.8 bits (1405), Expect = 2.1e-151
Identity = 264/268 (98.51%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLL MAFSGP FLHPSSASRLNPLLFTKHRRSF LSRFRFRRLHQRQLAVAASANGVFTS
Sbjct: 1   MLLLMAFSGPSFLHPSSASRLNPLLFTKHRRSFALSRFRFRRLHQRQLAVAASANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIYKWWESQGYFRPH+DQDTVPFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHYDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 269
           TAVSDLEVEYSEESGTLYHIKYRVAGGS
Sbjct: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGS 268

[Close]

BLAST of CSPI03G20930 vs. NCBI nr
Match: XP_038883468.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 535.4 bits (1378), Expect = 2.8e-148
Identity = 258/269 (95.91%), Postives = 261/269 (97.03%), Query Frame = 0


Query: 1   MLLQMAFSGPYFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQLAVAASANGVFTS 60
           MLLQMAFSGP  LHPSSA RLNPLLFT+HR SFGLSRF FRRL QRQLAVA SANGVFTS
Sbjct: 1   MLLQMAFSGPSLLHPSSACRLNPLLFTRHRPSFGLSRFHFRRLQQRQLAVATSANGVFTS 60

Query: 61  PEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVTLE 120
           PEIAKTFDFASEERIYKWWESQGYFRPH DQDT+PFVISMPPPNVTGSLHMGHAMFVTLE
Sbjct: 61  PEIAKTFDFASEERIYKWWESQGYFRPHFDQDTIPFVISMPPPNVTGSLHMGHAMFVTLE 120

Query: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180
           DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK
Sbjct: 121 DIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKRVWEWKEK 180

Query: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240
           YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ
Sbjct: 181 YGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSPNLQ 240

Query: 241 TAVSDLEVEYSEESGTLYHIKYRVAGGSR 270
           TAVSDLEVEYSEE+GTLYHIKYRVAGGSR
Sbjct: 241 TAVSDLEVEYSEEAGTLYHIKYRVAGGSR 269

[Close]

BLAST of CSPI03G20930 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 425.6 bits (1093), Expect = 2.9e-119
Identity = 209/273 (76.56%), Postives = 232/273 (84.98%), Query Frame = 0


Query: 1   MLLQMAFSGP----YFLHPSSASRLNPLLFTKHRRSFGLSRFRFRRLHQRQ---LAVAAS 60
           M+L+ AFS P      L PSS  +LN L FT+ RR   +S  R   +  ++    + AAS
Sbjct: 1   MILKTAFSLPTPTTTLLSPSSPHQLNTLFFTRRRRRL-ISPSRLNSIFSQRRFSFSAAAS 60

Query: 61  ANGVFTSPEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGH 120
            N VFTSPE +KTFDF+SEE+IYKWWESQGYF+P+ DQ   PFVI MPPPNVTGSLHMGH
Sbjct: 61  GNNVFTSPETSKTFDFSSEEKIYKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHMGH 120

Query: 121 AMFVTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKR 180
           AMFVTLEDIMVRYNRM GRPTLWLPGTDHAGIATQLVVE+MLASEGIKRV+LGRDEFTKR
Sbjct: 121 AMFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVDLGRDEFTKR 180

Query: 181 VWEWKEKYGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMV 240
           VWEWKEKYGGTITNQIKRLGASCDW++E FTLD+QLSRAV+EAFV+LH++GLIYQGSYMV
Sbjct: 181 VWEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMV 240

Query: 241 NWSPNLQTAVSDLEVEYSEESGTLYHIKYRVAG 267
           NWSPNLQTAVSDLEVEYSEE G LYHIKYRVAG
Sbjct: 241 NWSPNLQTAVSDLEVEYSEEPGFLYHIKYRVAG 272

[Close]

BLAST of CSPI03G20930 vs. TAIR 10
Match: AT1G14610.1 (valyl-tRNA synthetase / valine--tRNA ligase (VALRS) )

HSP 1 Score: 221.1 bits (562), Expect = 1.1e-57
Identity = 101/193 (52.33%), Postives = 140/193 (72.54%), Query Frame = 0


Query: 60  SPEIAKTFDFASEERI-YKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGHAMFVT 119
           S ++AK +  A+ E+  Y WWE    F+        PFVI +PPPNVTG+LH+GHA+   
Sbjct: 133 SSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSSKPPFVIVLPPPNVTGALHIGHALTSA 192

Query: 120 LEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVE-RMLASEGIKRVELGRDEFTKRVWEW 179
           +ED ++R+ RM G   LW+PG DHAGIATQ+VVE +++   G+ R ++GR+EF K VW+W
Sbjct: 193 IEDTIIRWKRMSGYNALWVPGVDHAGIATQVVVEKKIMRDRGMTRHDVGREEFVKEVWKW 252

Query: 180 KEKYGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMVNWSP 239
           K +YGGTI  Q++RLGAS DW++E FT+D+Q S+AV EAFVRL++ GLIY+   +VNW  
Sbjct: 253 KNQYGGTILTQLRRLGASLDWSRECFTMDEQRSKAVTEAFVRLYKEGLIYRDIRLVNWDC 312

Query: 240 NLQTAVSDLEVEY 251
            L+TA+SD+EVEY
Sbjct: 313 ILRTAISDVEVEY 325

[Close]

BLAST of CSPI03G20930 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.3e-23
Identity = 67/218 (30.73%), Postives = 110/218 (50.46%), Query Frame = 0


Query: 54  ANGVFTSPEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGH 113
           AN +   PE+ K            W E+Q + R   + +   F++   PP   G LHMGH
Sbjct: 117 ANSLTREPELQKL-----------WEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGH 176

Query: 114 AMFVTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKR 173
           A+   L+DI+ RY  ++     ++PG D  G+  +L V + L  E   R EL   +   +
Sbjct: 177 ALNKILKDIINRYKLLQNYKVQYVPGWDCHGLPIELKVLQSLDQE--VRKELTPLKLRAK 236

Query: 174 VWEWKEKYGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMV 233
             ++ +    T     KR G   DW   + TLD +   A IE F ++  +G IY+G   V
Sbjct: 237 AAKFAKATVKTQMESFKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPV 296

Query: 234 NWSPNLQTAVSDLEVEYSEE--SGTLYHIKYRVAGGSR 270
           +WSP+ +TA+++ E+EY E   S ++Y I +++ GG++
Sbjct: 297 HWSPSSRTALAEAELEYPEGHISKSIYAI-FKLVGGAK 320

[Close]

BLAST of CSPI03G20930 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.3e-23
Identity = 67/218 (30.73%), Postives = 110/218 (50.46%), Query Frame = 0


Query: 54  ANGVFTSPEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGH 113
           AN +   PE+ K            W E+Q + R   + +   F++   PP   G LHMGH
Sbjct: 117 ANSLTREPELQKL-----------WEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGH 176

Query: 114 AMFVTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKR 173
           A+   L+DI+ RY  ++     ++PG D  G+  +L V + L  E   R EL   +   +
Sbjct: 177 ALNKILKDIINRYKLLQNYKVQYVPGWDCHGLPIELKVLQSLDQE--VRKELTPLKLRAK 236

Query: 174 VWEWKEKYGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMV 233
             ++ +    T     KR G   DW   + TLD +   A IE F ++  +G IY+G   V
Sbjct: 237 AAKFAKATVKTQMESFKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPV 296

Query: 234 NWSPNLQTAVSDLEVEYSEE--SGTLYHIKYRVAGGSR 270
           +WSP+ +TA+++ E+EY E   S ++Y I +++ GG++
Sbjct: 297 HWSPSSRTALAEAELEYPEGHISKSIYAI-FKLVGGAK 320

[Close]

BLAST of CSPI03G20930 vs. TAIR 10
Match: AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.3e-23
Identity = 67/218 (30.73%), Postives = 110/218 (50.46%), Query Frame = 0


Query: 54  ANGVFTSPEIAKTFDFASEERIYKWWESQGYFRPHHDQDTVPFVISMPPPNVTGSLHMGH 113
           AN +   PE+ K            W E+Q + R   + +   F++   PP   G LHMGH
Sbjct: 303 ANSLTREPELQKL-----------WEENQVFKRVSDNNNGGSFILHDGPPYANGDLHMGH 362

Query: 114 AMFVTLEDIMVRYNRMKGRPTLWLPGTDHAGIATQLVVERMLASEGIKRVELGRDEFTKR 173
           A+   L+DI+ RY  ++     ++PG D  G+  +L V + L  E   R EL   +   +
Sbjct: 363 ALNKILKDIINRYKLLQNYKVQYVPGWDCHGLPIELKVLQSLDQE--VRKELTPLKLRAK 422

Query: 174 VWEWKEKYGGTITNQIKRLGASCDWTKEHFTLDDQLSRAVIEAFVRLHERGLIYQGSYMV 233
             ++ +    T     KR G   DW   + TLD +   A IE F ++  +G IY+G   V
Sbjct: 423 AAKFAKATVKTQMESFKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPV 482

Query: 234 NWSPNLQTAVSDLEVEYSEE--SGTLYHIKYRVAGGSR 270
           +WSP+ +TA+++ E+EY E   S ++Y I +++ GG++
Sbjct: 483 HWSPSSRTALAEAELEYPEGHISKSIYAI-FKLVGGAK 506

The following BLAST results are available for this feature:

BLAST of CSPI03G20930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KE63	4.1e-118	76.56	Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=37...	[more]
C1D6J9	9.3e-70	59.71	Valine--tRNA ligase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=valS...	[more]
Q6F8F0	1.9e-67	59.02	Valine--tRNA ligase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) O...	[more]
Q82X51	2.5e-67	58.29	Valine--tRNA ligase OS=Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / K...	[more]
Q0VSA8	1.3e-66	60.41	Valine--tRNA ligase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 /...	[more]

BLAST of CSPI03G20930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCI4	1.5e-155	100.00	tRNA-synt_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G285540...	[more]
A0A1S3C571	1.0e-151	98.51	Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1	[more]
A0A1S3C576	1.0e-151	98.51	Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1	[more]
A0A1S3C4H7	1.0e-151	98.51	Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103496791 PE=3 SV=1	[more]
A0A6J1IZ88	1.5e-147	95.54	Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=3 SV=1	[more]

BLAST of CSPI03G20930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651387.1	1.2e-154	100.00	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis sativus] ...	[more]
XP_008457007.1	2.1e-151	98.51	PREDICTED: valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucumi...	[more]
XP_008457012.1	2.1e-151	98.51	PREDICTED: valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X6 [Cucumi...	[more]
XP_008457006.1	2.1e-151	98.51	PREDICTED: valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumi...	[more]
XP_038883468.1	2.8e-148	95.91	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Benincasa hispida...	[more]

BLAST of CSPI03G20930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16715.1	2.9e-119	76.56	ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi...	[more]
AT1G14610.1	1.1e-57	52.33	valyl-tRNA synthetase / valine--tRNA ligase (VALRS)	[more]
AT5G49030.1	1.3e-23	30.73	tRNA synthetase class I (I, L, M and V) family protein	[more]
AT5G49030.2	1.3e-23	30.73	tRNA synthetase class I (I, L, M and V) family protein	[more]
AT5G49030.3	1.3e-23	30.73	tRNA synthetase class I (I, L, M and V) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 66..262 e-value: 7.4E-63 score: 214.9
IPR002300	Aminoacyl-tRNA synthetase, class Ia	PFAM	PF00133	tRNA-synt_1	coord: 75..267 e-value: 4.2E-72 score: 243.3
IPR002303	Valine-tRNA ligase	PANTHER	PTHR11946	VALYL-TRNA SYNTHETASES	coord: 62..266
None	No IPR available	PANTHER	PTHR11946:SF93	VALYL-TRNA SYNTHETASE, ISOFORM C	coord: 62..266
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 68..257
IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site	PROSITE	PS00178	AA_TRNA_LIGASE_I	coord: 103..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G20930.1	CSPI03G20930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0106074	aminoacyl-tRNA metabolism involved in translational fidelity
biological_process	GO:0006438	valyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005840	ribosome
molecular_function	GO:0002161	aminoacyl-tRNA editing activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004823	leucine-tRNA ligase activity
molecular_function	GO:0003735	structural constituent of ribosome
molecular_function	GO:0004832	valine-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding