CSPI03G20870 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G20870
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Valyl-tRNA synthetase
Location	Chr3: 16924843 .. 16927355 (-)
RNA-Seq Expression	CSPI03G20870
Synteny	CSPI03G20870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCAACCTGGGAGACTTATGAAGATTTGTAGCAATGTTTTCCAGAATTTCACCTTGAATTTGGAGGAGTGTAATGATAGACCCCCTATCATACACCAATATAGTAGGAGAAGAAAAAAGAATTGACACGTGCGAATGAGGGAGAGGGTTTACGTGAGAGTAAAGTAGTTAGGAAGTATTAATAGTGGAAAGGAGAGGGCGGGAAAGTTAGTTAAGCTTTTGGATAGAGAAGCGTTTCTGTTCTACCTTGAGAGATAGGTTAGCAGAAGGGAGTTCTTACATCCTTGAGATCGGATTAGAGATTGTAACCTAGATTTATCCTTGAATCTAATATCAATGATACTATTACTTACTATTCAATAAAGTTCCTGGTTTCCATCAGCTCACCACCAGACCGTGGGTGGGTAATGGAAAATAAATCTCGTAAAATTCAATCTTCTAAATCAACCCATGCAGAATTTCTGAATCTTCAAATTGTGGTAGCCCTATTTCATATCTCATTGCTGACTCTATTTTATCATACCTAGTGGCACAACTGATGTAAATCTCTTGCGGAAGTCATAAAACAATTATAACTTGTTTTTACTGCCTGCTGCAGGAAGAGAAGGAAGTTTTAGCTCTTCTTACTCGACTGGATCTACATAATGTCCATTTTGCAAATTCTCCTCCAGGCATGTCTATTACTTGAGTTATTTTTCTCATTTTTGTACCAGTATCTATTCGCATGCATATTTGTGGGTGATGGTTTCCGTGCCTTGTTAAATCATTTACCTAAGTTGGGTTAGACTGTTGAAGTATTATTTTTGCTTTATGTTTTTTTCCGCAGGAAATGTAGATCAGTCGGTGCACCTCGTTGCTGGTGAAGGGCTAGAGGCTTATCTACCTCTTGCTGATATGGTTGATATTTCTGCCGAAGTCCAACGCTTATCGAAGCGCCTTACAAAAATGAAAATAGAGTATGATGGATTTATAGCTCGGCTCAGTTCTCCAAGTGTACGTTACTTGCTCAGAAACATATTTCAGATTAGTATCTATTTACATGTTTTTTGCCTGAAATAGAATACATATGAAATAGGTAACTCTACATAATATTGAAGTTTTCAGTTAGTAAAAGATAAACTAGTACAGAATTGTTTCTTCCTTGTATCTGCCTCGTCATTTCTTAATGATTCCGGGCTGCTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGTTTGGGAACCCATTCCCTATACAACAATTGTTAGAACCTTAAATCTGTCATCTTGCTCAGAACAACCAAGTACGAGTCTAGGTCCAGATCCTTTGTAAGTTGTATTCGTATTTATGTAATTCATGTTATTTACAATTTCAGCTTTTAAGGTTCGGAGGAGTTCACTGTATATAAACTCTTTAGAACTTTGACTCATTTTTCAGTCACGTCCTCATACTTATCCAACAACATCAAGTCAAATCTTACAAATTGAAAAGTGATCATTGTGAACAACGTGGCAAGGGAGGTCCAAATCAATTTGACTTTGTATAGTTGAATTTAGTATTGCATTTATATATCAGTGCTTTTCTTACAGCCACTTTTTAGTGACTCACACCCGGATGAACACCCTGTTTTTCTGTTTATTTGAAATGAGGGACTGATATAGCAAATACATTTTATTTTACATTTGATATTCATTAGTCAATGCGTTGGATATGTAAGCTTCTGATTTCAACCTAAAATGATGTTACATTTTAATCTATTCACCAGAACAAGACTTCAGCTTTTTTCCTTTAAGAAATACATAAACTGAAAGGGATTTTAATCACACCAAAACATATTATAGCTCTTGCGGTCTGGTTTGATCTTGCAGTTTGTAGAGAAAGCTCCCGAGGACATCGTCCGTGGTGTAAGAGAAAAGGCTGAAGAAGCGAAAGAGAAGATAGCTCTCACCGAGAAGCGCCTTTCTCTCCTTGGATCAACTGTTCCAGTTCCTGATTGTTGACCATCACCAAAAATTGGTTCCAGTTGTATGTAAACTGTTCAATTATCAAAGAAGTAACAAGCAATTGATTGAGGCCAATTTATGCGAGGCTTTCTCGAGTTGTAAGAGCTCCATTTTTCTGGTGAATTAACATGCCCCTCCATCCCATTGAGTTTGTTCAGTAAAGGCTGAGCAACCCTCAGGCTAACCCCAGAAATAGAGGCTGAGTTGAATCATACCAAATAGTTAAAAACTTGAAATCAACTCTTTCTTTATGTTAGTTTATAGCCTAGGAAATAGTTTTTTAAAAACGATTCTTCACTTCGTTGATTTTATTATTTTTCCATTTGAATATTAACTTGTAATATGTATTTTTAGCCTTCCGCTGATTCTTTTTGCCATTTGTAGTCGTATGTGGTGGAGATGAAGCTGAGTGTCATCATCAATGTAGGCTTTCCATTATAAAGAAACAAGTATCCATGAACTACTTGATGTAAATGAGTTAATTCTAAGATTTGACATCCAACTTATTACTTATCCAGGTAAATTTATAAC

mRNA sequence

ATGAAGCAACCTGGGAGACTTATGAAGATTTGTAGCAATGTTTTCCAGAATTTCACCTTGAATTTGGAGGAGTGTAATGATAGACCCCCTATCATACACCAATATAAGAAGCGTTTCTGTTCTACCTTGAGAGATAGGTTAGCAGAAGGGAGTTCTTACATCCTTGAGATCGGATTAGAGATTTTCCTGGTTTCCATCAGCTCACCACCAGACCGTGGGTGGGTAATGGAAAATAAATCTCGTAAAATTCAATCTTCTAAATCAACCCATGCAGAATTTCTGAATCTTCAAATTGTGGAAGAGAAGGAAGTTTTAGCTCTTCTTACTCGACTGGATCTACATAATGTCCATTTTGCAAATTCTCCTCCAGGAAATGTAGATCAGTCGGTGCACCTCGTTGCTGGTGAAGGGCTAGAGGCTTATCTACCTCTTGCTGATATGGTTGATATTTCTGCCGAAGTCCAACGCTTATCGAAGCGCCTTACAAAAATGAAAATAGAGTATGATGGATTTATAGCTCGGCTCAGTTCTCCAAGTTTTGTAGAGAAAGCTCCCGAGGACATCGTCCGTGGTGTAAGAGAAAAGGCTGAAGAAGCGAAAGAGAAGATAGCTCTCACCGAGAAGCGCCTTTCTCTCCTTGGATCAACTGTTCCAGTTCCTGATTGTTGACCATCACCAAAAATTGGTTCCAGTTGTATGTAAACTGTTCAATTATCAAAGAAGTAACAAGCAATTGATTGAGGCCAATTTATGCGAGGCTTTCTCGAGTTGTAAGAGCTCCATTTTTCTGGTGAATTAACATGCCCCTCCATCCCATTGAGTTTGTTCAGTAAAGGCTGAGCAACCCTCAGGCTAACCCCAGAAATAGAGGCTGAGTTGAATCATACCAAATAGTTAAAAACTTGAAATCAACTCTTTCTTTATGTTAGTTTATAGCCTAGGAAATAGTTTTTTAAAAACGATTCTTCACTTCGTTGATTTTATTATTTTTCCATTTGAATATTAACTTGTAATATGTATTTTTAGCCTTCCGCTGATTCTTTTTGCCATTTGTAGTCGTATGTGGTGGAGATGAAGCTGAGTGTCATCATCAATGTAGGCTTTCCATTATAAAGAAACAAGTATCCATGAACTACTTGATGTAAATGAGTTAATTCTAAGATTTGACATCCAACTTATTACTTATCCAGGTAAATTTATAAC

Coding sequence (CDS)

ATGAAGCAACCTGGGAGACTTATGAAGATTTGTAGCAATGTTTTCCAGAATTTCACCTTGAATTTGGAGGAGTGTAATGATAGACCCCCTATCATACACCAATATAAGAAGCGTTTCTGTTCTACCTTGAGAGATAGGTTAGCAGAAGGGAGTTCTTACATCCTTGAGATCGGATTAGAGATTTTCCTGGTTTCCATCAGCTCACCACCAGACCGTGGGTGGGTAATGGAAAATAAATCTCGTAAAATTCAATCTTCTAAATCAACCCATGCAGAATTTCTGAATCTTCAAATTGTGGAAGAGAAGGAAGTTTTAGCTCTTCTTACTCGACTGGATCTACATAATGTCCATTTTGCAAATTCTCCTCCAGGAAATGTAGATCAGTCGGTGCACCTCGTTGCTGGTGAAGGGCTAGAGGCTTATCTACCTCTTGCTGATATGGTTGATATTTCTGCCGAAGTCCAACGCTTATCGAAGCGCCTTACAAAAATGAAAATAGAGTATGATGGATTTATAGCTCGGCTCAGTTCTCCAAGTTTTGTAGAGAAAGCTCCCGAGGACATCGTCCGTGGTGTAAGAGAAAAGGCTGAAGAAGCGAAAGAGAAGATAGCTCTCACCGAGAAGCGCCTTTCTCTCCTTGGATCAACTGTTCCAGTTCCTGATTGTTGA

Protein sequence

MKQPGRLMKICSNVFQNFTLNLEECNDRPPIIHQYKKRFCSTLRDRLAEGSSYILEIGLEIFLVSISSPPDRGWVMENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLLGSTVPVPDC*

Homology

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BLAST of CSPI03G20870 vs. ExPASy Swiss-Prot
Match: F4KE63 (Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2247 PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 5.7e-41
Identity = 89/136 (65.44%), Postives = 110/136 (80.88%), Query Frame = 0


Query: 81  RKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEA 140
           ++I +S    AE +   I +EKEVLALL+RLDL+NVHF+N+PPG+ + SVHLVA EGLEA
Sbjct: 834 KRISASVVGSAEVIE-YISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEA 893

Query: 141 YLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAK 200
           YLPLA MVDIS+EVQR+SKRL+KM+ EYD  I RLSSP FVEKAPE++VRGV+E+ EE +
Sbjct: 894 YLPLAAMVDISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELE 953

Query: 201 EKIALTEKRLSLLGST 217
           EKI LT+ RL  L ST
Sbjct: 954 EKIKLTKARLDFLKST 968

[Close]

BLAST of CSPI03G20870 vs. ExPASy Swiss-Prot
Match: Q5NHZ4 (Valine--tRNA ligase OS=Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) OX=177416 GN=valS PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 6.2e-11
Identity = 41/102 (40.20%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 113 LHNVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFI 172
           ++N+ F ++PP ++ Q V     EGLE  +PLA +VDI AE  RL K L K+K E D   
Sbjct: 823 INNIEFNDNPPTSLSQIV-----EGLELNIPLAGLVDIEAEKARLDKELDKLKDEVDRVQ 882

Query: 173 ARLSSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLLG 215
            +LS+  FV  APE +V   +EK  + +E  A T ++   LG
Sbjct: 883 KKLSNERFVSNAPEAVVAAEQEKLAKYQELYAKTLEKKEALG 919

[Close]

BLAST of CSPI03G20870 vs. ExPASy Swiss-Prot
Match: Q97GG8 (Valine--tRNA ligase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=valS PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.8e-10
Identity = 35/99 (35.35%), Postives = 58/99 (58.59%), Query Frame = 0


Query: 115 NVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIAR 174
           +V F  S     + +V  V  +G E ++PL D++D++ E++RLSK   K+K E      +
Sbjct: 783 SVSFLKSKDEAPENAVSSVT-KGAELFMPLLDLIDVTKEIERLSKEKDKLKAEIQRVDKK 842

Query: 175 LSSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 214
           LS+  FV+KAPE +V   R K E+ K+ +   E+R++ L
Sbjct: 843 LSNKGFVDKAPESVVEAERVKGEKYKKMLEAVEERIAAL 880

[Close]

BLAST of CSPI03G20870 vs. ExPASy Swiss-Prot
Match: Q9X2D7 (Valine--tRNA ligase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=valS PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 9.8e-09
Identity = 37/112 (33.04%), Postives = 60/112 (53.57%), Query Frame = 0


Query: 99  VEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRLS 158
           V E+E L L T  ++  V F N  P    ++      E +EAY+ L  ++D   E +RL 
Sbjct: 753 VTEEEELLLKTLGNIEEVSFVNEKP---PKTATAYVEEEIEAYVDLGGLIDFEKEKERLK 812

Query: 159 KRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRL 211
           + + K++ E D    +L++  FVEKAPE++V   +EK    +E++A  E  L
Sbjct: 813 QIMEKIQKEIDRLEKKLANKDFVEKAPEEVVEETKEKLNTNRERLARLESIL 861

[Close]

BLAST of CSPI03G20870 vs. ExPASy Swiss-Prot
Match: Q891R5 (Valine--tRNA ligase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=valS PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.7e-08
Identity = 35/98 (35.71%), Postives = 57/98 (58.16%), Query Frame = 0


Query: 116 VHFANSPPGNVDQSVHLVAGEGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARL 175
           V F NS     + +V  V  +G E Y+PL D+VD+  E++RL+K   K++ E +    +L
Sbjct: 783 VEFLNSKEEAPENAVSAVT-KGAEIYIPLFDLVDLEKEMERLNKEREKLEKEIERVDKKL 842

Query: 176 SSPSFVEKAPEDIVRGVREKAEEAKEKIALTEKRLSLL 214
           S+ +FV+KAPE +V   + K E+ KE +    +R+  L
Sbjct: 843 SNENFVKKAPEAVVNEEKAKGEKYKEMLEAVLERIKSL 879

[Close]

BLAST of CSPI03G20870 vs. ExPASy TrEMBL
Match: A0A0A0L9W5 (Val_tRNA-synt_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G285030 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 5.9e-73
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 76  MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAG 135
           MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAG
Sbjct: 1   MENKSRKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAG 60

Query: 136 EGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK 195
           EGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK
Sbjct: 61  EGLEAYLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREK 120

Query: 196 AEEAKEKIALTEKRLSLLGSTVPVPDC 223
           AEEAKEKIALTEKRLSLLGSTVPVPDC
Sbjct: 121 AEEAKEKIALTEKRLSLLGSTVPVPDC 147

[Close]

BLAST of CSPI03G20870 vs. ExPASy TrEMBL
Match: A0A6J1IZ88 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 7.0e-58
Identity = 121/138 (87.68%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 85  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 144
           S+    +E +N  I EEKEVLALLTRLDLHNVHFAN+PPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 832 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPL 891

Query: 145 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 204
           ADMVDISAE+QRLSKRLTKMK EYDGFIARL+SPSFVEKAPEDIVRGVREKA+EAKEKIA
Sbjct: 892 ADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIA 951

Query: 205 LTEKRLSLLGSTVPVPDC 223
           LTEKRLSLL STVPVPDC
Sbjct: 952 LTEKRLSLLRSTVPVPDC 969

[Close]

BLAST of CSPI03G20870 vs. ExPASy TrEMBL
Match: A0A6J1J7Q1 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 7.0e-58
Identity = 121/138 (87.68%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 85  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 144
           S+    +E +N  I EEKEVLALLTRLDLHNVHFAN+PPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 686 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPL 745

Query: 145 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 204
           ADMVDISAE+QRLSKRLTKMK EYDGFIARL+SPSFVEKAPEDIVRGVREKA+EAKEKIA
Sbjct: 746 ADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIA 805

Query: 205 LTEKRLSLLGSTVPVPDC 223
           LTEKRLSLL STVPVPDC
Sbjct: 806 LTEKRLSLLRSTVPVPDC 823

[Close]

BLAST of CSPI03G20870 vs. ExPASy TrEMBL
Match: A0A6J1F580 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 7.0e-58
Identity = 121/138 (87.68%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 85  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 144
           S+    +E +N  I EEKEVLALLTRLDLHNVHFAN+PPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 832 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPL 891

Query: 145 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 204
           ADMVDISAE+QRLSKRLTKMK EYDGFIARL+SPSFVEKAPEDIVRGVREKA+EAKEKIA
Sbjct: 892 ADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIA 951

Query: 205 LTEKRLSLLGSTVPVPDC 223
           LTEKRLSLL STVPVPDC
Sbjct: 952 LTEKRLSLLRSTVPVPDC 969

[Close]

BLAST of CSPI03G20870 vs. ExPASy TrEMBL
Match: A0A6J1FA83 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 7.0e-58
Identity = 121/138 (87.68%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 85  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 144
           S+    +E +N  I EEKEVLALLTRLDLHNVHFAN+PPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 686 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPL 745

Query: 145 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 204
           ADMVDISAE+QRLSKRLTKMK EYDGFIARL+SPSFVEKAPEDIVRGVREKA+EAKEKIA
Sbjct: 746 ADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIA 805

Query: 205 LTEKRLSLLGSTVPVPDC 223
           LTEKRLSLL STVPVPDC
Sbjct: 806 LTEKRLSLLRSTVPVPDC 823

[Close]

BLAST of CSPI03G20870 vs. NCBI nr
Match: XP_011651387.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis sativus] >KAE8650589.1 hypothetical protein Csa_011094 [Cucumis sativus])

HSP 1 Score: 243.4 bits (620), Expect = 1.8e-60
Identity = 127/138 (92.03%), Postives = 130/138 (94.20%), Query Frame = 0


Query: 85  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 144
           S+    +E +N  I EEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 832 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 891

Query: 145 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 204
           ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA
Sbjct: 892 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 951

Query: 205 LTEKRLSLLGSTVPVPDC 223
           LTEKRLSLLGSTVPVPDC
Sbjct: 952 LTEKRLSLLGSTVPVPDC 969

[Close]

BLAST of CSPI03G20870 vs. NCBI nr
Match: XP_011651390.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 243.4 bits (620), Expect = 1.8e-60
Identity = 127/138 (92.03%), Postives = 130/138 (94.20%), Query Frame = 0


Query: 85  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 144
           S+    +E +N  I EEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 686 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 745

Query: 145 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 204
           ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA
Sbjct: 746 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 805

Query: 205 LTEKRLSLLGSTVPVPDC 223
           LTEKRLSLLGSTVPVPDC
Sbjct: 806 LTEKRLSLLGSTVPVPDC 823

[Close]

BLAST of CSPI03G20870 vs. NCBI nr
Match: XP_038883468.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 233.8 bits (595), Expect = 1.4e-57
Identity = 122/138 (88.41%), Postives = 127/138 (92.03%), Query Frame = 0


Query: 85  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 144
           S+    +E +N  I EEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 832 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 891

Query: 145 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 204
           ADMVDISAEVQRLSKRLTKMK EYDGF+ARL+S SFVEKAPEDIVRGVREKAEEAKEKIA
Sbjct: 892 ADMVDISAEVQRLSKRLTKMKTEYDGFVARLNSSSFVEKAPEDIVRGVREKAEEAKEKIA 951

Query: 205 LTEKRLSLLGSTVPVPDC 223
           LTEKRLSLL STVPVPDC
Sbjct: 952 LTEKRLSLLRSTVPVPDC 969

[Close]

BLAST of CSPI03G20870 vs. NCBI nr
Match: XP_022983417.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 1.4e-57
Identity = 121/138 (87.68%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 85  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 144
           S+    +E +N  I EEKEVLALLTRLDLHNVHFAN+PPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 832 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPL 891

Query: 145 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 204
           ADMVDISAE+QRLSKRLTKMK EYDGFIARL+SPSFVEKAPEDIVRGVREKA+EAKEKIA
Sbjct: 892 ADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIA 951

Query: 205 LTEKRLSLLGSTVPVPDC 223
           LTEKRLSLL STVPVPDC
Sbjct: 952 LTEKRLSLLRSTVPVPDC 969

[Close]

BLAST of CSPI03G20870 vs. NCBI nr
Match: XP_022935323.1 (valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 233.8 bits (595), Expect = 1.4e-57
Identity = 121/138 (87.68%), Postives = 128/138 (92.75%), Query Frame = 0


Query: 85  SSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEAYLPL 144
           S+    +E +N  I EEKEVLALLTRLDLHNVHFAN+PPGNVDQSVHLVAGEGLEAYLPL
Sbjct: 686 SASIVASEEVNQYISEEKEVLALLTRLDLHNVHFANAPPGNVDQSVHLVAGEGLEAYLPL 745

Query: 145 ADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAKEKIA 204
           ADMVDISAE+QRLSKRLTKMK EYDGFIARL+SPSFVEKAPEDIVRGVREKA+EAKEKIA
Sbjct: 746 ADMVDISAELQRLSKRLTKMKTEYDGFIARLNSPSFVEKAPEDIVRGVREKADEAKEKIA 805

Query: 205 LTEKRLSLLGSTVPVPDC 223
           LTEKRLSLL STVPVPDC
Sbjct: 806 LTEKRLSLLRSTVPVPDC 823

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BLAST of CSPI03G20870 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 169.1 bits (427), Expect = 4.1e-42
Identity = 89/136 (65.44%), Postives = 110/136 (80.88%), Query Frame = 0


Query: 81  RKIQSSKSTHAEFLNLQIVEEKEVLALLTRLDLHNVHFANSPPGNVDQSVHLVAGEGLEA 140
           ++I +S    AE +   I +EKEVLALL+RLDL+NVHF+N+PPG+ + SVHLVA EGLEA
Sbjct: 834 KRISASVVGSAEVIE-YISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEA 893

Query: 141 YLPLADMVDISAEVQRLSKRLTKMKIEYDGFIARLSSPSFVEKAPEDIVRGVREKAEEAK 200
           YLPLA MVDIS+EVQR+SKRL+KM+ EYD  I RLSSP FVEKAPE++VRGV+E+ EE +
Sbjct: 894 YLPLAAMVDISSEVQRISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELE 953

Query: 201 EKIALTEKRLSLLGST 217
           EKI LT+ RL  L ST
Sbjct: 954 EKIKLTKARLDFLKST 968

The following BLAST results are available for this feature:

BLAST of CSPI03G20870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KE63	5.7e-41	65.44	Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=37...	[more]
Q5NHZ4	6.2e-11	40.20	Valine--tRNA ligase OS=Francisella tularensis subsp. tularensis (strain SCHU S4 ...	[more]
Q97GG8	6.8e-10	35.35	Valine--tRNA ligase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / J...	[more]
Q9X2D7	9.8e-09	33.04	Valine--tRNA ligase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 1...	[more]
Q891R5	1.7e-08	35.71	Valine--tRNA ligase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717...	[more]

BLAST of CSPI03G20870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9W5	5.9e-73	100.00	Val_tRNA-synt_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G28...	[more]
A0A6J1IZ88	7.0e-58	87.68	Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=3 SV=1	[more]
A0A6J1J7Q1	7.0e-58	87.68	Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111482028 PE=4 SV=1	[more]
A0A6J1F580	7.0e-58	87.68	Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=3 SV=1	[more]
A0A6J1FA83	7.0e-58	87.68	Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111442245 PE=4 SV=1	[more]

BLAST of CSPI03G20870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651387.1	1.8e-60	92.03	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis sativus] ...	[more]
XP_011651390.1	1.8e-60	92.03	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucumis sativus]	[more]
XP_038883468.1	1.4e-57	88.41	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Benincasa hispida...	[more]
XP_022983417.1	1.4e-57	87.68	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita maxima]	[more]
XP_022935323.1	1.4e-57	87.68	valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucurbita moschat...	[more]

BLAST of CSPI03G20870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16715.1	4.1e-42	65.44	ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 189..209
None	No IPR available	COILS	Coil	Coil	coord: 147..167
None	No IPR available	PANTHER	PTHR11946:SF93	VALYL-TRNA SYNTHETASE, ISOFORM C	coord: 81..215
IPR037118	Valyl-tRNA synthetase, tRNA-binding arm superfamily	GENE3D	1.10.287.380		coord: 142..214 e-value: 2.4E-19 score: 71.1
IPR019499	Valyl-tRNA synthetase, tRNA-binding arm	PFAM	PF10458	Val_tRNA-synt_C	coord: 149..213 e-value: 3.1E-15 score: 56.2
IPR002303	Valine-tRNA ligase	PANTHER	PTHR11946	VALYL-TRNA SYNTHETASES	coord: 81..215
IPR010978	Class I and II aminoacyl-tRNA synthetase, tRNA-binding arm	SUPERFAMILY	46589	tRNA-binding arm	coord: 149..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G20870.1	CSPI03G20870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0106074	aminoacyl-tRNA metabolism involved in translational fidelity
biological_process	GO:0006438	valyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0002161	aminoacyl-tRNA editing activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004823	leucine-tRNA ligase activity
molecular_function	GO:0004832	valine-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding