CSPI03G20360 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G20360
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTPX2 domain-containing protein
LocationChr3: 16166272 .. 16169067 (-)
RNA-Seq ExpressionCSPI03G20360
SyntenyCSPI03G20360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTAACATATTTGGATTATTCTTCTCTCTTTCATTTCCCCCTCCCTCATCCACTCATGGACAAGTCCCAACCAAAATCTTTGATGACCAAGGTTTGTGACACTTTTGATCCTACAGCCACTTCTTGGTTTTCTCTTGTTCTAACCTTTTTTTGTTATTAGACGAAAGATGGAGTCCGAGACCGAGTTTTAGAGAAAGCGGAGAGAGCTCGAGTAAGTTTTAGTGATCATTGGAAGTTGAAGTTTTTTGCCCTTGTTTGTGGAATGAAAAGTATGATTAGAGGTTTGTTTTCCTAATTATTTTTAGGTTCCTCAAAAGGCTCATGTGAAGGAAAACACCAAGAAGTCCCAAGATTTCAAGTTGCATACGCAAGAAAGAGCGGTGAAACGAGCAATGTTTAACTATTCGGTGAGTTCATGGTCGTGGAAGTGTTTAGGTTCATTGAGAATTTAATATAATATCAAAATTATATTGAGAGAGCTATTCATGTGTAGTATATTGTTTTCTCTTTCATTAATAATAATGATCGCTTGTTGGGTTTTGTGTTAATTTTTCAAGCAGATAGCGACAAAGTTATATGTAACGGAGCTACAAAAGAAAGTGGAAGAAAAGTTGCACAAGGTAACAACTTACGAATTAAATCTTTTGAATATATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAAACTTGTTTTGGAATAAAATAGTGTAGAGTAAACAATTACTAAAGAAACAAAATATATTGTGAAGCAGATGATAGAAGAGGAAGAGGTTCGTTTGATGAGAAAGGAAATGATACCAAGAGCTCAACTCATGCCCTACTTTGATAGACCCTACTTTCCACAAAGGTAGTGCACAAAATAGCTTTTATAAATTATATAATATTTTTAAATAACGTTTAGACGTCCATTCTAGACTGCATCTATCTTAATTATAAGAATATCCATCCTTAGTTATTACTTAATAAACCCTTTTAAGTATATACAAAAAAAAACTCTCTTTTATATTTACTCTTTTCCTTTGTGATTGAAATATATACATGGAGATATGAGAAATGTAGCATGAGCTTCATCCAAAGTATTGTATCTACATTTTTTTAAAACAATGACTTTTATAGGGGGCAAGAATGAGATTGAGTTTTGCACCTACTGATCTCTTTCTCAGTTGTATCTCTTTCTGTTGTGTATGATTGTTAGAAAAAAGTGGTAGATCAAAAAGGTACTTTTAATAATTCCATAAGTGTTTACTGATTTATTACATAGTTCAAAAGTCATATTTGAAAGTAATACAAGTGCACACCCCAATGCTTGATTGTTATGAGGAAAAGTAAAACGTTTTTTAAAAGTATCAATGAAACTATATCTTGATGTCCTAAAGAAACTTTTCACTTTTAAAATGCTATTTTCTTGTAAAAAAAGAAAGGATTGTGATTATTTGTAATGCAGATCGAATCGACCTTTGACGATACCGAGAGAGCCGAGTTTCTTGATGAACAAAGAGCAATGGAGTTGTAACACTGACAGTGAACTTTACAGCTTTCAAAGACAAGCTTTGAAACCAATCAAATAAAATCCCAACTATATTTGATCTATTATTGTATAGATTTTGGGTTTTGTGTTTAATTTTTCTGATTCTATTTGATGCATCTCATCTTTGTACAATTTATTTAGTGGTAGAAAGAGAGAGAGAGAGTGTGTGATGTTTTTGAGGCAGAGAAGAAGCATTATTGTTGCTTTCTGACTTTTGTTCTTTCAAATGTGTCAAAATAAAATCCACTGTTTATTACACAACTAATAAACTATTTCCAAATTGAGAAC

mRNA sequence

CTCTAACATATTTGGATTATTCTTCTCTCTTTCATTTCCCCCTCCCTCATCCACTCATGGACAAGTCCCAACCAAAATCTTTGATGACCAAGACGAAAGATGGAGTCCGAGACCGAGTTTTAGAGAAAGCGGAGAGAGCTCGAGTTCCTCAAAAGGCTCATGTGAAGGAAAACACCAAGAAGTCCCAAGATTTCAAGTTGCATACGCAAGAAAGAGCGGTGAAACGAGCAATGTTTAACTATTCGATAGCGACAAAGTTATATGTAACGGAGCTACAAAAGAAAGTGGAAGAAAAGTTGCACAAGATGATAGAAGAGGAAGAGGTTCGTTTGATGAGAAAGGAAATGATACCAAGAGCTCAACTCATGCCCTACTTTGATAGACCCTACTTTCCACAAAGATCGAATCGACCTTTGACGATACCGAGAGAGCCGAGTTTCTTGATGAACAAAGAGCAATGGAGTTGTAACACTGACAGTGAACTTTACAGCTTTCAAAGACAAGCTTTGAAACCAATCAAATAAAATCCCAACTATATTTGATCTATTATTGTATAGATTTTGGGTTTTGTGTTTAATTTTTCTGATTCTATTTGATGCATCTCATCTTTGTACAATTTATTTAGTGGTAGAAAGAGAGAGAGAGAGTGTGTGATGTTTTTGAGGCAGAGAAGAAGCATTATTGTTGCTTTCTGACTTTTGTTCTTTCAAATGTGTCAAAATAAAATCCACTGTTTATTACACAACTAATAAACTATTTCCAAATTGAGAAC

Coding sequence (CDS)

ATGGACAAGTCCCAACCAAAATCTTTGATGACCAAGACGAAAGATGGAGTCCGAGACCGAGTTTTAGAGAAAGCGGAGAGAGCTCGAGTTCCTCAAAAGGCTCATGTGAAGGAAAACACCAAGAAGTCCCAAGATTTCAAGTTGCATACGCAAGAAAGAGCGGTGAAACGAGCAATGTTTAACTATTCGATAGCGACAAAGTTATATGTAACGGAGCTACAAAAGAAAGTGGAAGAAAAGTTGCACAAGATGATAGAAGAGGAAGAGGTTCGTTTGATGAGAAAGGAAATGATACCAAGAGCTCAACTCATGCCCTACTTTGATAGACCCTACTTTCCACAAAGATCGAATCGACCTTTGACGATACCGAGAGAGCCGAGTTTCTTGATGAACAAAGAGCAATGGAGTTGTAACACTGACAGTGAACTTTACAGCTTTCAAAGACAAGCTTTGAAACCAATCAAATAA

Protein sequence

MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMFNYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK*
Homology
BLAST of CSPI03G20360 vs. ExPASy Swiss-Prot
Match: F4I2H7 (Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 4.9e-07
Identity = 39/133 (29.32%), Postives = 74/133 (55.64%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           M+  + +  + K +  +++  +   E+ R+P          + Q+F LH + RAV+RA F
Sbjct: 610 METEEAQKRLFKAQPVIKEDPIPVPEKVRMP--------LTEIQEFNLHVEHRAVERADF 669

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEE-VRLMRKEMIPRAQLMPYFDRPYFPQRSNRP 120
           ++ I  K    +  ++ E +  KM+EEE  ++ MRK M+P A+ +P F++P+ PQ+SN+ 
Sbjct: 670 DHKIKEKENQYKRYRE-ESEAAKMVEEERALKQMRKTMVPHARPVPNFNKPFLPQKSNKG 729

Query: 121 LTIPREPSFLMNK 133
            T  + P+  + K
Sbjct: 730 TTKAKSPNLRVIK 733

BLAST of CSPI03G20360 vs. ExPASy TrEMBL
Match: A0A0A0LCD4 (TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G258180 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.9e-78
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF
Sbjct: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 156
           TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 155

BLAST of CSPI03G20360 vs. ExPASy TrEMBL
Match: A0A6J1K9I8 (protein TPX2 OS=Cucurbita maxima OX=3661 GN=LOC111492897 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.3e-68
Identity = 139/155 (89.68%), Postives = 143/155 (92.26%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           MDKS PKS + K KDG+RDRV +K ER R PQK  VKENTKK QDFKLHTQERAVKRAMF
Sbjct: 1   MDKSPPKSFI-KAKDGIRDRVSDKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYSIATKLYV ELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVMELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 156
           TIPREPSFLMNKEQWSCNTDSE+YSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNTDSEVYSFQRQALKPIK 154

BLAST of CSPI03G20360 vs. ExPASy TrEMBL
Match: A0A6J1GBJ5 (protein TPX2-like OS=Cucurbita moschata OX=3662 GN=LOC111452663 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 8.9e-68
Identity = 139/155 (89.68%), Postives = 142/155 (91.61%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           MDKS PKS + K KDGVRDRV EK ER R PQK  VKENTKK QDFKLHTQERAVKRAMF
Sbjct: 1   MDKSPPKSFI-KAKDGVRDRVSEKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 156
           TIPREPSFL+NKEQWSCN DSE+YSFQRQ LKPIK
Sbjct: 121 TIPREPSFLINKEQWSCNGDSEVYSFQRQTLKPIK 154

BLAST of CSPI03G20360 vs. ExPASy TrEMBL
Match: A0A6J1CNR5 (protein TPX2 OS=Momordica charantia OX=3673 GN=LOC111013295 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 3.2e-65
Identity = 134/155 (86.45%), Postives = 144/155 (92.90%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           MDK QP+SL+ K KDGVRDRV E+AERAR  QK  VKENT K QDFKLHTQERAVKRAMF
Sbjct: 1   MDKFQPRSLI-KVKDGVRDRVSERAERARGSQKVVVKENT-KMQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYS+ATK YVTE+QKK+EEKLHKMIEEEE+RL+RKEM+PRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSVATKSYVTEIQKKIEEKLHKMIEEEEIRLLRKEMVPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 156
           TIPREPSFL+NKEQWSCNTDSELYSFQRQALKPIK
Sbjct: 121 TIPREPSFLVNKEQWSCNTDSELYSFQRQALKPIK 153

BLAST of CSPI03G20360 vs. ExPASy TrEMBL
Match: A0A5D3DX27 (Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00390 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 5.8e-51
Identity = 112/115 (97.39%), Postives = 112/115 (97.39%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           MDKSQPKSLMTKTKDGVRDRVLEK ERARVPQKA VKENTKK QDFKLHTQERAVKRAMF
Sbjct: 1   MDKSQPKSLMTKTKDGVRDRVLEKVERARVPQKAPVKENTKKPQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 116
           NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQR 115

BLAST of CSPI03G20360 vs. NCBI nr
Match: XP_004145652.1 (protein TPX2 [Cucumis sativus])

HSP 1 Score: 301.6 bits (771), Expect = 3.9e-78
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF
Sbjct: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 156
           TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 155

BLAST of CSPI03G20360 vs. NCBI nr
Match: XP_038877019.1 (protein TPX2-like [Benincasa hispida])

HSP 1 Score: 285.4 bits (729), Expect = 2.9e-73
Identity = 148/156 (94.87%), Postives = 149/156 (95.51%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQK-AHVKENTKKSQDFKLHTQERAVKRAM 60
           MDKSQPKS MTKTKDGVRDRVLEKAER R PQK  HVKENTKK QDFKLHTQERAVKRAM
Sbjct: 1   MDKSQPKSFMTKTKDGVRDRVLEKAERVRAPQKVVHVKENTKKPQDFKLHTQERAVKRAM 60

Query: 61  FNYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRP 120
           FNYSIATKLYV ELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRP
Sbjct: 61  FNYSIATKLYVMELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRP 120

Query: 121 LTIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 156
           LTIPREPSFLMNKEQW+CNTDSELYSFQRQALKPIK
Sbjct: 121 LTIPREPSFLMNKEQWNCNTDSELYSFQRQALKPIK 156

BLAST of CSPI03G20360 vs. NCBI nr
Match: XP_022998171.1 (protein TPX2 [Cucurbita maxima])

HSP 1 Score: 268.1 bits (684), Expect = 4.8e-68
Identity = 139/155 (89.68%), Postives = 143/155 (92.26%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           MDKS PKS + K KDG+RDRV +K ER R PQK  VKENTKK QDFKLHTQERAVKRAMF
Sbjct: 1   MDKSPPKSFI-KAKDGIRDRVSDKTERVRTPQKVVVKENTKKPQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYSIATKLYV ELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVMELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 156
           TIPREPSFLMNKEQWSCNTDSE+YSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNTDSEVYSFQRQALKPIK 154

BLAST of CSPI03G20360 vs. NCBI nr
Match: KAG7036751.1 (Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 268.1 bits (684), Expect = 4.8e-68
Identity = 140/155 (90.32%), Postives = 143/155 (92.26%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           MDKS PKS + K KDGVRDRV EK ER R PQK  VK+NTKK QDFKLHTQERAVKRAMF
Sbjct: 1   MDKSPPKSFI-KAKDGVRDRVSEKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 156
           TIPREPSFLMNKEQWSCN DSE+YSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 154

BLAST of CSPI03G20360 vs. NCBI nr
Match: XP_023525926.1 (protein TPX2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 266.9 bits (681), Expect = 1.1e-67
Identity = 139/155 (89.68%), Postives = 143/155 (92.26%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           MDKS PKS + K KDGVRDRV +K ER R PQK  VK+NTKK QDFKLHTQERAVKRAMF
Sbjct: 1   MDKSPPKSFI-KAKDGVRDRVSDKTERVRTPQKVVVKDNTKKPQDFKLHTQERAVKRAMF 60

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120
           NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL
Sbjct: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPL 120

Query: 121 TIPREPSFLMNKEQWSCNTDSELYSFQRQALKPIK 156
           TIPREPSFLMNKEQWSCN DSE+YSFQRQALKPIK
Sbjct: 121 TIPREPSFLMNKEQWSCNGDSEVYSFQRQALKPIK 154

BLAST of CSPI03G20360 vs. TAIR 10
Match: AT5G37478.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 141.4 bits (355), Expect = 6.3e-34
Identity = 78/140 (55.71%), Postives = 106/140 (75.71%), Query Frame = 0

Query: 10  MTKTKDGVRDRVLEKAERARVPQ--KAHVKENTKKSQDFKLHTQERAVKRAMFNYSIATK 69
           MTK  + +++R  +K + A      KA  KEN KK  +FKLH+ ERAVKRAMFNYS+AT 
Sbjct: 34  MTKV-EPLKERAHDKTKAATTKNITKAPAKEN-KKPLEFKLHSGERAVKRAMFNYSVATN 93

Query: 70  LYVTELQKKVEEKLHKMIEEEEVRLMRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPS 129
            Y+ +LQKK EE+L KMIEEEE+R++RKEM+P+AQLMP+FDRP+ PQRS+RPLT+P+EPS
Sbjct: 94  YYIQKLQKKQEERLQKMIEEEEIRMLRKEMVPKAQLMPFFDRPFLPQRSSRPLTMPKEPS 153

Query: 130 F-LMNKEQWSCNTDSELYSF 147
           F  +N   W+C  +++ Y +
Sbjct: 154 FGNVNSTCWTCVFNNQHYLY 171

BLAST of CSPI03G20360 vs. TAIR 10
Match: AT3G01015.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-19
Identity = 52/111 (46.85%), Postives = 75/111 (67.57%), Query Frame = 0

Query: 33  KAHVKENTKKSQDFKLHTQERAVKRAMFNYSIATKLYVTELQKKVEEKLHKMIEEEEVRL 92
           K HVK+ T    D KLH+  RAV+RA F+Y +  K+ + E  K   E+  K+ EEEE+R 
Sbjct: 362 KPHVKDIT-IPVDLKLHSDIRAVERAEFDYQVTEKINLVEQYKTERERQQKLAEEEEIRR 421

Query: 93  MRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSFLM--NKEQWSCNTDS 142
           +RKE++P+AQ MPYFDRP+ P+RSN+  T+PR+P F +  +K+   C+T S
Sbjct: 422 LRKELVPKAQPMPYFDRPFIPKRSNKHPTVPRDPKFNIPQHKKIRCCSTSS 471

BLAST of CSPI03G20360 vs. TAIR 10
Match: AT5G15510.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 87.8 bits (216), Expect = 8.3e-18
Identity = 54/128 (42.19%), Postives = 75/128 (58.59%), Query Frame = 0

Query: 33  KAHVKENTKKSQDFKLHTQERAVKRAMFNYSIATKLYVTELQKKVEEKLHKMIEEEEVRL 92
           K H K+ T +  D  LH+  RAV+RA F+Y +A K+   E  K   E+  K+ EEEE+R 
Sbjct: 372 KPHWKDIT-RPVDLTLHSDVRAVERAEFDYQVAEKMSFIEQYKMERERQQKLAEEEEIRR 431

Query: 93  MRKEMIPRAQLMPYFDRPYFPQRSNRPLTIPREPSF---------LMNKEQWSCNTDSEL 152
           +RKE++P+AQ MPYFDRP+ P+RS++  T PR+P F           +   WS  T S +
Sbjct: 432 LRKELVPKAQPMPYFDRPFIPRRSSKHPTAPRDPKFHIPQHKKIRCCSSSSWS-ETGSCM 491

BLAST of CSPI03G20360 vs. TAIR 10
Match: AT5G15510.2 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 73.6 bits (179), Expect = 1.6e-13
Identity = 40/83 (48.19%), Postives = 56/83 (67.47%), Query Frame = 0

Query: 33  KAHVKENTKKSQDFKLHTQERAVKRAMFNYSIATKLYVTELQKKVEEKLHKMIEEEEVRL 92
           K H K+ T +  D  LH+  RAV+RA F+Y +A K+   E  K   E+  K+ EEEE+R 
Sbjct: 372 KPHWKDIT-RPVDLTLHSDVRAVERAEFDYQVAEKMSFIEQYKMERERQQKLAEEEEIRR 431

Query: 93  MRKEMIPRAQLMPYFDRPYFPQR 116
           +RKE++P+AQ MPYFDRP+ P+R
Sbjct: 432 LRKELVPKAQPMPYFDRPFIPRR 453

BLAST of CSPI03G20360 vs. TAIR 10
Match: AT1G03780.2 (targeting protein for XKLP2 )

HSP 1 Score: 55.8 bits (133), Expect = 3.5e-08
Identity = 39/133 (29.32%), Postives = 74/133 (55.64%), Query Frame = 0

Query: 1   MDKSQPKSLMTKTKDGVRDRVLEKAERARVPQKAHVKENTKKSQDFKLHTQERAVKRAMF 60
           M+  + +  + K +  +++  +   E+ R+P          + Q+F LH + RAV+RA F
Sbjct: 610 METEEAQKRLFKAQPVIKEDPIPVPEKVRMP--------LTEIQEFNLHVEHRAVERADF 669

Query: 61  NYSIATKLYVTELQKKVEEKLHKMIEEEE-VRLMRKEMIPRAQLMPYFDRPYFPQRSNRP 120
           ++ I  K    +  ++ E +  KM+EEE  ++ MRK M+P A+ +P F++P+ PQ+SN+ 
Sbjct: 670 DHKIKEKENQYKRYRE-ESEAAKMVEEERALKQMRKTMVPHARPVPNFNKPFLPQKSNKG 729

Query: 121 LTIPREPSFLMNK 133
            T  + P+  + K
Sbjct: 730 TTKAKSPNLRVIK 733

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I2H74.9e-0729.32Protein TPX2 OS=Arabidopsis thaliana OX=3702 GN=TPX2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LCD41.9e-78100.00TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G258180 PE=3 S... [more]
A0A6J1K9I82.3e-6889.68protein TPX2 OS=Cucurbita maxima OX=3661 GN=LOC111492897 PE=3 SV=1[more]
A0A6J1GBJ58.9e-6889.68protein TPX2-like OS=Cucurbita moschata OX=3662 GN=LOC111452663 PE=3 SV=1[more]
A0A6J1CNR53.2e-6586.45protein TPX2 OS=Momordica charantia OX=3673 GN=LOC111013295 PE=3 SV=1[more]
A0A5D3DX275.8e-5197.39Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00390 P... [more]
Match NameE-valueIdentityDescription
XP_004145652.13.9e-78100.00protein TPX2 [Cucumis sativus][more]
XP_038877019.12.9e-7394.87protein TPX2-like [Benincasa hispida][more]
XP_022998171.14.8e-6889.68protein TPX2 [Cucurbita maxima][more]
KAG7036751.14.8e-6890.32Protein TPX2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023525926.11.1e-6789.68protein TPX2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G37478.16.3e-3455.71TPX2 (targeting protein for Xklp2) protein family [more]
AT3G01015.11.2e-1946.85TPX2 (targeting protein for Xklp2) protein family [more]
AT5G15510.18.3e-1842.19TPX2 (targeting protein for Xklp2) protein family [more]
AT5G15510.21.6e-1348.19TPX2 (targeting protein for Xklp2) protein family [more]
AT1G03780.23.5e-0829.32targeting protein for XKLP2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 70..90
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..45
NoneNo IPR availablePANTHERPTHR14326:SF39TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILYcoord: 3..147
IPR027329TPX2, C-terminalPFAMPF06886TPX2coord: 46..127
e-value: 4.3E-20
score: 71.7
IPR009675TPX2PANTHERPTHR14326TARGETING PROTEIN FOR XKLP2coord: 3..147

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G20360.1CSPI03G20360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032147 activation of protein kinase activity
biological_process GO:0090307 mitotic spindle assembly
biological_process GO:0060236 regulation of mitotic spindle organization
cellular_component GO:0005737 cytoplasm
cellular_component GO:0072686 mitotic spindle
cellular_component GO:0005880 nuclear microtubule
cellular_component GO:0005874 microtubule
cellular_component GO:0005819 spindle
molecular_function GO:0008017 microtubule binding
molecular_function GO:0030295 protein kinase activator activity