CSPI03G17580 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G17580
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cytochrome P450
Location	Chr3: 13182918 .. 13185062 (-)
RNA-Seq Expression	CSPI03G17580
Synteny	CSPI03G17580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTCCCGTTTTCCAAAAATCAAAACTATGATAATTTACCTAACAATCTTTTTCTGTCTTCTAATCCCCATCTTCCTCTTCCTTCGAAGATCATCCAAAAAGCGGCAGCTTCCTCCAGGTTCGCTAGGGTTTCCATTGATCGGTCAGAGTCTCAGCCTCCTCCGCGCCATGCGAACCAACACCGCTGAACAATGGGCGCAGCAGAGGATCAGAAACTACGGGGCCATCTCCAAGCTAACCCTATTCGGAAAGCCAACTGTGTTCATCGGTGGGCAGAGCGGGAACAAGTTGATTTTCAGCGGGGATTGCGCAGTAGTTTCGAATCAGCAAAATGAGTCTCTTAGAGCAATTTTAGGGGAAAGGAATTTGTTGGAGCTGATTGGGGAAGATCATAAGCGTGTGAGAAATGCGTTGGTTTCGTTCTTGAAGCCGGAATGTTTGAAGGAATATGTGGGCAAAATGGATGAAGAAATCAGATCACATATCCGCATGCATTGGCACTGTAAACAAGAGGTTACTGTGAGTACACCTTTTCCTACTTTTTCTTTTTTCTTACGTTTTTCAAAATTGAAAATTGGTATTTCAATTTTCATTTCTGAGTTCTCAACAGATATACACATTTCTACTATTGTTTGAAAAAATAATAATAAAGAACCCCGTTGCCGGGGATAAAAAACGGTAATACCCACTGCCACACTACAACGACTTAATTTGTTACATTAACTAAATAAAGAATATATAAAACGAGTTTAAAATAAATAAAAAATTGAGAGTTGAATGTGTTATTGTAATTATGATGTTTTGTCTATTGAGATGATGTTTATTATGCACATAGGTATTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGTTCTCTTCTGTTTGGTCTTGAGCAAGGTACGAGAAGAGAAAGAATGATAGAATGCTTTCGAGTAATGATAAGCGGAGTATGGTCGATCCCGATAAACTTGCCGTTCACAAGGTACAACCAAAGCCGGCGTGCGAGTAGAAAAATTCAAGAGATGCTGAAGGAATTGTTGGATGAAAAGAGAGTTGAATTAGAAGAAAAAGGAGGCTCCTCACACCAAGATCTAATTACTTGTTTGTTAAGCATAAGGAATGAAGAAAATGAACTAGTATTGAGTGAAAAGGAAATTGTGCACAATATTATGTTGGTATTGGTTGCTGGATTTGATACTTCATCGGTTCTCATTACGTTCATGATGCGAAATTTAGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGGTATGCTGCTTCCAATTATCTTATACACTCTTTATATTACAATGACTGTATATATAATCACATCTTTCTTCAAGCTTATTTGTTTTTTGTTGTGGTGTGTGTGTGTGTGGTTAATTACAGAACAAGAAGAGATAGCAAGAAGTAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAGTAAGTCAAACTTTCTTCCTTTTTTGACTTGAGGATCTCAAGTGAAACTTTATGTAACATCTAATAAACGTATTCATCCAACCACACTATTGGAATTCAGATATTTTGGACATCACCAGTTACTCATTTGGATGACACCATATTTAGAGAACCATCAAAATTCGACCCAGATAGGTTCAAAAATCCAGCGTCCATTCCACCGTATTGCTTCCTTGGATTTGGCAGCGGTCCGAGAATTTGTCCAGGCAATGAGTTTGCTAGGGTTGAGACTCTTGTTACCATACATTACTTGATCACCCAATTCACATGGAAACTACTTTTGGATGACCATTTTATCAGAGATCCAATGCCAACACCTACCAAAGGAATGCCTATCAAAATTTGGCCCAGATCACAATCTTTCATGGCAGTGTAGAATGCGTAGAAATGAGAATCATGGTTAGTGACATATTTGGGAGTGAGAAGATCTCAAAAATTGTTAAAATCACCTGTCTTGTTTAAAATCATTCGGATAGTATGTAAAAAATAAGTTCGGGCGTAAAATCAAACATTAAATTAT

mRNA sequence

ATTTTCCCGTTTTCCAAAAATCAAAACTATGATAATTTACCTAACAATCTTTTTCTGTCTTCTAATCCCCATCTTCCTCTTCCTTCGAAGATCATCCAAAAAGCGGCAGCTTCCTCCAGGTTCGCTAGGGTTTCCATTGATCGGTCAGAGTCTCAGCCTCCTCCGCGCCATGCGAACCAACACCGCTGAACAATGGGCGCAGCAGAGGATCAGAAACTACGGGGCCATCTCCAAGCTAACCCTATTCGGAAAGCCAACTGTGTTCATCGGTGGGCAGAGCGGGAACAAGTTGATTTTCAGCGGGGATTGCGCAGTAGTTTCGAATCAGCAAAATGAGTCTCTTAGAGCAATTTTAGGGGAAAGGAATTTGTTGGAGCTGATTGGGGAAGATCATAAGCGTGTGAGAAATGCGTTGGTTTCGTTCTTGAAGCCGGAATGTTTGAAGGAATATGTGGGCAAAATGGATGAAGAAATCAGATCACATATCCGCATGCATTGGCACTGTAAACAAGAGGTTACTGTATTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGTTCTCTTCTGTTTGGTCTTGAGCAAGGTACGAGAAGAGAAAGAATGATAGAATGCTTTCGAGTAATGATAAGCGGAGTATGGTCGATCCCGATAAACTTGCCGTTCACAAGGTACAACCAAAGCCGGCGTGCGAGTAGAAAAATTCAAGAGATGCTGAAGGAATTGTTGGATGAAAAGAGAGTTGAATTAGAAGAAAAAGGAGGCTCCTCACACCAAGATCTAATTACTTGTTTGTTAAGCATAAGGAATGAAGAAAATGAACTAGTATTGAGTGAAAAGGAAATTGTGCACAATATTATGTTGGTATTGGTTGCTGGATTTGATACTTCATCGGTTCTCATTACGTTCATGATGCGAAATTTAGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGAACAAGAAGAGATAGCAAGAAGTAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAATATTTTGGACATCACCAGTTACTCATTTGGATGACACCATATTTAGAGAACCATCAAAATTCGACCCAGATAGGTTCAAAAATCCAGCGTCCATTCCACCGTATTGCTTCCTTGGATTTGGCAGCGGTCCGAGAATTTGTCCAGGCAATGAGTTTGCTAGGGTTGAGACTCTTGTTACCATACATTACTTGATCACCCAATTCACATGGAAACTACTTTTGGATGACCATTTTATCAGAGATCCAATGCCAACACCTACCAAAGGAATGCCTATCAAAATTTGGCCCAGATCACAATCTTTCATGGCAGTGTAGAATGCGTAGAAATGAGAATCATGGTTAGTGACATATTTGGGAGTGAGAAGATCTCAAAAATTGTTAAAATCACCTGTCTTGTTTAAAATCATTCGGATAGTATGTAAAAAATAAGTTCGGGCGTAAAATCAAACATTAAATTAT

Coding sequence (CDS)

ATGATAATTTACCTAACAATCTTTTTCTGTCTTCTAATCCCCATCTTCCTCTTCCTTCGAAGATCATCCAAAAAGCGGCAGCTTCCTCCAGGTTCGCTAGGGTTTCCATTGATCGGTCAGAGTCTCAGCCTCCTCCGCGCCATGCGAACCAACACCGCTGAACAATGGGCGCAGCAGAGGATCAGAAACTACGGGGCCATCTCCAAGCTAACCCTATTCGGAAAGCCAACTGTGTTCATCGGTGGGCAGAGCGGGAACAAGTTGATTTTCAGCGGGGATTGCGCAGTAGTTTCGAATCAGCAAAATGAGTCTCTTAGAGCAATTTTAGGGGAAAGGAATTTGTTGGAGCTGATTGGGGAAGATCATAAGCGTGTGAGAAATGCGTTGGTTTCGTTCTTGAAGCCGGAATGTTTGAAGGAATATGTGGGCAAAATGGATGAAGAAATCAGATCACATATCCGCATGCATTGGCACTGTAAACAAGAGGTTACTGTATTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGTTCTCTTCTGTTTGGTCTTGAGCAAGGTACGAGAAGAGAAAGAATGATAGAATGCTTTCGAGTAATGATAAGCGGAGTATGGTCGATCCCGATAAACTTGCCGTTCACAAGGTACAACCAAAGCCGGCGTGCGAGTAGAAAAATTCAAGAGATGCTGAAGGAATTGTTGGATGAAAAGAGAGTTGAATTAGAAGAAAAAGGAGGCTCCTCACACCAAGATCTAATTACTTGTTTGTTAAGCATAAGGAATGAAGAAAATGAACTAGTATTGAGTGAAAAGGAAATTGTGCACAATATTATGTTGGTATTGGTTGCTGGATTTGATACTTCATCGGTTCTCATTACGTTCATGATGCGAAATTTAGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGAACAAGAAGAGATAGCAAGAAGTAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAATATTTTGGACATCACCAGTTACTCATTTGGATGACACCATATTTAGAGAACCATCAAAATTCGACCCAGATAGGTTCAAAAATCCAGCGTCCATTCCACCGTATTGCTTCCTTGGATTTGGCAGCGGTCCGAGAATTTGTCCAGGCAATGAGTTTGCTAGGGTTGAGACTCTTGTTACCATACATTACTTGATCACCCAATTCACATGGAAACTACTTTTGGATGACCATTTTATCAGAGATCCAATGCCAACACCTACCAAAGGAATGCCTATCAAAATTTGGCCCAGATCACAATCTTTCATGGCAGTGTAG

Protein sequence

MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV*

Homology

[Close]

BLAST of CSPI03G17580 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.5e-102
Identity = 194/474 (40.93%), Postives = 282/474 (59.49%), Query Frame = 0


Query: 3   IYLTIFFCLLIPIFLFL-----RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWA 62
           +  T+F    I +  F       R+   R +PPG+ G+PLIG++L  L   R      + 
Sbjct: 21  LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFF 80

Query: 63  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 122
             R + YG +   +L G PTV      GN+ +FS +  +V N    S+   L   +L+  
Sbjct: 81  DSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSV-GNLFRSSLITT 140

Query: 123 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIV 182
           +G+D KR+R  L++FL+PE L+E+VG++D   + H+  HW  K EV  LPL+K  TF++ 
Sbjct: 141 VGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLA 200

Query: 183 CSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEK 242
           C L   +      +R+   F V + GV  IPI+LP TRYN+++ A+  I++ L  +++E+
Sbjct: 201 CDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINER 260

Query: 243 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMR 302
           ++ LE    S  QDL++ LLS  +E+ E  L++ EI  NI+L+L AG DTSS  +T +++
Sbjct: 261 KIALEAGNASPEQDLLSFLLSNVDEQGE-SLTDNEIQDNILLLLYAGHDTSSSTLTVLLK 320

Query: 303 NLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRK 362
            LA NP  Y  VL+EQ  IA SK+ G+LL WEDL +MKY+WRVA E LRL   + G FRK
Sbjct: 321 FLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRK 380

Query: 363 AMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSG 422
           A+ +  + G+ IPKGW++ WT   TH     F  P KFDP RF+     PPY F+ FG G
Sbjct: 381 AIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGP-PPYTFVPFGGG 440

Query: 423 PRICPGNEFARVETLVTIHYLITQFTWKLLLD-DHFIRDPMPTPTKGMPIKIWP 471
           PR+CPGNEFAR+E L+ +H ++  F W L+   +  I DPMP P  G+PIK+ P
Sbjct: 441 PRMCPGNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

[Close]

BLAST of CSPI03G17580 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 5.6e-102
Identity = 194/480 (40.42%), Postives = 283/480 (58.96%), Query Frame = 0


Query: 3   IYLTIFFCLLIPIFLFL-----RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWA 62
           +  T+F    I +  F       R+   R +PPG+ G+PLIG++L  L   R      + 
Sbjct: 21  LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFF 80

Query: 63  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 122
             R + YG +   +L G PTV      GN+ +FS +  +V N    S+   L   +L+  
Sbjct: 81  DSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSV-GNLFRSSLITT 140

Query: 123 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIV 182
           +G+D KR+R  L++FL+PE L+E+VG++D   + H+  HW  K EVT LPL+K  TF++ 
Sbjct: 141 VGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLA 200

Query: 183 CSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEK 242
           C L   +       R+   F V + GV  IPI+LP TRYN+++ A+  I++ L  +++E+
Sbjct: 201 CDLFASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINER 260

Query: 243 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMR 302
           ++ LE    S  QDL++ LLS  +E+ E  L++ EI  NI+L+L AG DTSS  +T +++
Sbjct: 261 KIGLEAGNASPEQDLLSFLLSNVDEQGE-SLTDNEIQDNILLLLYAGHDTSSSTLTVLLK 320

Query: 303 NLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRK 362
            LA NP  Y  VL+EQ +IA SK+ G+LL WEDL +MKY+WRVA E LRL     G FRK
Sbjct: 321 FLAENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRK 380

Query: 363 AMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSG 422
           A+ +  + G+ IPKGW+++WT   TH     F  P  FDP RF+     PPY F+ FG G
Sbjct: 381 AIKEFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEGP-PPYTFVPFGGG 440

Query: 423 PRICPGNEFARVETLVTIHYLITQFTWKLL-LDDHFIRDPMPTPTKGMPIKIWPRSQSFM 477
           PR+CPGNEFAR+E LV +H ++    W L+   +  I DPMP P  G+PIK+ P    ++
Sbjct: 441 PRMCPGNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVPHDSVYI 497

[Close]

BLAST of CSPI03G17580 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 7.8e-96
Identity = 180/474 (37.97%), Postives = 288/474 (60.76%), Query Frame = 0


Query: 2   IIYLTIFFCLLIPIFLFLRRSSKKRQ---LPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQ 61
           ++ L + F  L   FLF +  S       LPPG  G+P+IG+S   L        E++  
Sbjct: 8   LLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKGYPEKFIF 67

Query: 62  QRIRNYGA-ISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 121
            R+  Y + + K +LFG+P     G + NK +FS +  +V     +S+  +         
Sbjct: 68  DRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFPSSTQTS- 127

Query: 122 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIV 181
             E+  ++R  L +FLKPE L+ YVG MD+    H +  W  K+EV V PL K  TF I 
Sbjct: 128 SKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKNYTFWIA 187

Query: 182 CSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEK 241
           C +   +E+ T+  +++E F  + SG+ S+PI+LP T +N + ++S+ +++ L  ++ ++
Sbjct: 188 CKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLMGIIKQR 247

Query: 242 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMR 301
           + +L E   S  QD+++ +L + ++EN   ++E +I   I+ +L+ G DT+S   TF+++
Sbjct: 248 KTDLGEGKASPTQDILSHML-LTSDENGKFMTEGDIADKILGLLIGGHDTASSACTFVVK 307

Query: 302 NLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRK 361
            LA  P +Y  V +EQ EIA+SKK GELLNWED+ KMKY+W VA E LRL  P+ G FR+
Sbjct: 308 FLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQGAFRE 367

Query: 362 AMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSG 421
           A+ D  + G+ IPKGW+++W++  TH +  +F EP KFDP RF+  A  PPY F+ FG G
Sbjct: 368 ALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEG-AGPPPYSFVPFGGG 427

Query: 422 PRICPGNEFARVETLVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 471
           PR+CPG E+AR+E LV +H+++ +F W K++ D+  + +PMP P KG+P++++P
Sbjct: 428 PRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFP 478

[Close]

BLAST of CSPI03G17580 vs. ExPASy Swiss-Prot
Match: A0A0U2U8U5 (Beta-amyrin 28-monooxygenase OS=Barbarea vulgaris OX=50459 GN=CYP716A81 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 8.7e-95
Identity = 178/481 (37.01%), Postives = 293/481 (60.91%), Query Frame = 0


Query: 3   IYLTIFFCLLIPIFLFLRRSSKK-------RQLPPGSLGFPLIGQSLSLLRAMRTNTAEQ 62
           +YLTI F  +  I L L    +K       + LPPG  GFPLIG+SLS L   R    E+
Sbjct: 1   MYLTILFLFVSSILLSLMFLLRKHLSHFSYQNLPPGKTGFPLIGESLSFLSEGRQGRPEK 60

Query: 63  WAQQRIRNY----GAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGE 122
           +   R+R +      + K  LFG PT  + G SGNK +F+ +  +V +   +S++ I   
Sbjct: 61  FVTDRVRRFISSSTGVFKTHLFGYPTAVMTGASGNKFLFTNENKLVVSWWPDSVKKIFPY 120

Query: 123 RNLLELIGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKT 182
                   ++ K++R  L+ F+KPE L++Y+G MDE  + H    W  ++++ V PL K 
Sbjct: 121 TQ--STYTDESKKLRILLMQFMKPEALRKYIGVMDEVAQRHFETLWTNQKQLIVYPLSKK 180

Query: 183 LTFNIVCSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLK 242
           LTF++ C L   ++   R  ++ + F  +++G++S+PI+LP TR+N+S +ASR +++ + 
Sbjct: 181 LTFSVACRLFLSMDDPERVSKLEDRFNSVVTGIYSVPIDLPGTRFNRSIKASRLLRKEVC 240

Query: 243 ELLDEKRVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVL 302
            ++ ++R EL+    S+ QD+++ +L    E       ++++ + ++ +L+ G DT+++ 
Sbjct: 241 AIIGQRREELKAGRASAEQDVLSHMLMSVGE-----TKDEDLANYLIGILIGGHDTAAIA 300

Query: 303 ITFMMRNLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPI 362
            TF++  LA  P VY  VLQEQ+EI + KK  E L WED+ KMKY+W VA E +RLV P+
Sbjct: 301 TTFIINYLAEYPHVYQRVLQEQKEILKEKKEEERLKWEDIEKMKYSWNVACEVMRLVPPL 360

Query: 363 FGGFRKAMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCF 422
            G FR+A++   F G+ IPKGW+++W++  TH++   F EP +F+P+RF+  +   PY +
Sbjct: 361 TGNFREAIDHFTFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEG-SGPKPYSY 420

Query: 423 LGFGSGPRICPGNEFARVETLVTIHYLITQFTWKLLL--DDHFIRDPMPTPTKGMPIKIW 471
           + FG GPR+CPG EFAR+E LV IH L+ +F W+ +   ++  + DP+P P  G+PI+I+
Sbjct: 421 IPFGGGPRMCPGREFARLEILVIIHNLVNRFKWEKVFPNENKIVVDPLPKPGNGLPIRIF 473

[Close]

BLAST of CSPI03G17580 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.1e-94
Identity = 183/471 (38.85%), Postives = 285/471 (60.51%), Query Frame = 0


Query: 2   IIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQRI 61
           ++ L + F  L   F+F ++ S    LPPG +G+P+IG+SL  L        E++   R+
Sbjct: 9   LLLLFVTFISLSLFFIFYKQKS-PLNLPPGKMGYPIIGESLEFLSTGWKGHPEKFIFDRM 68

Query: 62  RNYGA-ISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 121
           R Y + + K ++ G+ TV   G + NK +FS +  +V+    +S+  I    +L   + E
Sbjct: 69  RKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTSLDSNLKE 128

Query: 122 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 181
           +  ++R  L  F KPE L+ YVG MD   + H   HW  K E+TV PL K  TF + C L
Sbjct: 129 ESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYTFLLACRL 188

Query: 182 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 241
              +E      +  + F+++ +G+ S+PI+LP T +N++ +AS  I++ L +++ ++RV+
Sbjct: 189 FMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKIIKQRRVD 248

Query: 242 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 301
           L E   S  QD+++ +L + ++EN   ++E  I   I+ +L+ G DT+SV  TF+++ L 
Sbjct: 249 LAEGTASPTQDILSHML-LTSDENGKSMNELNIADKILGLLIGGHDTASVACTFLVKYLG 308

Query: 302 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 361
             P +Y  V QEQ EIA+SK  GELLNW+DL KMKY+W VA E +RL  P+ GGFR+A+ 
Sbjct: 309 ELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGGFREAIT 368

Query: 362 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 421
           D  F G+ IPKGW+++W++  TH +   F  P KFDP RF+      PY F+ FG GPR+
Sbjct: 369 DFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGP-APYTFVPFGGGPRM 428

Query: 422 CPGNEFARVETLVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 471
           CPG E+AR+E LV +H L+ +F W K++ D+  I DP P P K +PI+++P
Sbjct: 429 CPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYP 476

[Close]

BLAST of CSPI03G17580 vs. ExPASy TrEMBL
Match: A0A0A0LA02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.5e-278
Identity = 478/478 (100.00%), Postives = 478/478 (100.00%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60
           MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR
Sbjct: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240
           LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300

Query: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420
           DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 479
           CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 478

[Close]

BLAST of CSPI03G17580 vs. ExPASy TrEMBL
Match: A0A5A7TZG7 (Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00020 PE=3 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.0e-267
Identity = 461/477 (96.65%), Postives = 466/477 (97.69%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60
           MIIYLTI FCLLIPIFLFLR SSKK+ LPPGSLGFP IGQSLSLLRAMRTNTAEQWAQQR
Sbjct: 1   MIIYLTILFCLLIPIFLFLRTSSKKQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL GE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHI MHWH KQEVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240
           LFGLEQG RRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLV+VAGFDTSSVLITFMMR+LA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLA 300

Query: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420
           DIQFGGYLIPKGWQIFWTSPVTHLDD IFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMA 478
           CPGNEFARVETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKIWPR Q+FMA
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR-QTFMA 476

[Close]

BLAST of CSPI03G17580 vs. ExPASy TrEMBL
Match: A0A1S3C4V0 (cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.0e-267
Identity = 461/477 (96.65%), Postives = 466/477 (97.69%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60
           MIIYLTI FCLLIPIFLFLR SSKK+ LPPGSLGFP IGQSLSLLRAMRTNTAEQWAQQR
Sbjct: 1   MIIYLTILFCLLIPIFLFLRTSSKKQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL GE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHI MHWH KQEVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240
           LFGLEQG RRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLV+VAGFDTSSVLITFMMR+LA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLA 300

Query: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420
           DIQFGGYLIPKGWQIFWTSPVTHLDD IFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMA 478
           CPGNEFARVETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKIWPR Q+FMA
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR-QTFMA 476

[Close]

BLAST of CSPI03G17580 vs. ExPASy TrEMBL
Match: A0A6J1CC35 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 GN=LOC111010306 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.6e-224
Identity = 391/477 (81.97%), Postives = 425/477 (89.10%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFL---RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWA 60
           M IYLTI F  LIPIFLFL   RRS   ++LPPGSLG P IGQSL LLRAMR+NTAEQW 
Sbjct: 1   MNIYLTI-FSFLIPIFLFLTRGRRSRSSQKLPPGSLGVPFIGQSLGLLRAMRSNTAEQWL 60

Query: 61  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 120
           Q+RI  YG ISKLTLFGKPTV +GGQ  NKLIF+GD A +SNQQNESLRAILGE NLLEL
Sbjct: 61  QRRITKYGPISKLTLFGKPTVLVGGQGANKLIFNGDTA-ISNQQNESLRAILGESNLLEL 120

Query: 121 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIV 180
            G+DHKRVR+ALV FLKPECLK+YVGKMDEE+R H+ MHW  KQEVTVLPLMKTLTFNIV
Sbjct: 121 TGDDHKRVRSALVXFLKPECLKQYVGKMDEEVRRHLEMHWQXKQEVTVLPLMKTLTFNIV 180

Query: 181 CSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEK 240
           CSLLFGLE+GTRRERMIECF+VMI GVWSIPINLPFTRYNQSR+AS KIQEMLK LLDEK
Sbjct: 181 CSLLFGLEEGTRRERMIECFQVMIGGVWSIPINLPFTRYNQSRQASIKIQEMLKNLLDEK 240

Query: 241 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMR 300
           RVELE KG S HQDLITCLLSI+N+E+E VL+EKEI+HNI+LV+VAG+DTSSVLITFM+R
Sbjct: 241 RVELEVKGTSPHQDLITCLLSIKNKEHEQVLTEKEIIHNIILVMVAGYDTSSVLITFMVR 300

Query: 301 NLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRK 360
            LANNPTVYAAVLQEQEEIARSK+CGELL WEDLAKMKYTWRVALETLRLVAP+FGGFRK
Sbjct: 301 FLANNPTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRK 360

Query: 361 AMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSG 420
           AM DI+FGGYLIPKGWQIFW SP+THLDDTIF EP KFDP+RF+N AS+P YCF+ FG G
Sbjct: 361 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGG 420

Query: 421 PRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQS 475
           PRICPG EFARVETLVTIHYLITQFTWKLLLDD FIRDPMP PTKG+PIK+ PR  S
Sbjct: 421 PRICPGYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETS 475

[Close]

BLAST of CSPI03G17580 vs. ExPASy TrEMBL
Match: A0A5J5A0L0 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_010117 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.9e-202
Identity = 333/472 (70.55%), Postives = 412/472 (87.29%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60
           M I+ TIF  LL   FL +RR S  ++LPPGSLG P+IGQSLSLL+AM+ NTAE+W ++R
Sbjct: 15  MNIFFTIFLFLLPIFFLLVRRRSSSQRLPPGSLGIPIIGQSLSLLQAMKANTAERWLEER 74

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IR YG+ISKL+LFGKPTVFI GQ+ NKL+F+GDC+V++NQQ +S+R ILG+RNLLEL GE
Sbjct: 75  IRKYGSISKLSLFGKPTVFIYGQAANKLVFTGDCSVITNQQAQSVRMILGDRNLLELSGE 134

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180
           DHKRVR+ALVSFLKPECLK+YVGKM+EEIR H+ MHW  KQ+VTVLPLMKTLTFNI+CSL
Sbjct: 135 DHKRVRDALVSFLKPECLKQYVGKMEEEIREHLEMHWEGKQKVTVLPLMKTLTFNIICSL 194

Query: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240
           LFG+++G RR++++E F+ MI G+WS+P+NLPFTRYN+S RA+ ++Q MLK+L+ EKRVE
Sbjct: 195 LFGVQRGARRDKLVEYFQEMIQGMWSVPVNLPFTRYNRSLRATARVQNMLKDLIREKRVE 254

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300
           LE++G SSHQDLITCLLSIR ++NE ++SEKEIVHN+MLV+VAG DTSSVLI+F+MR L+
Sbjct: 255 LEQQGASSHQDLITCLLSIRGKDNEELISEKEIVHNVMLVMVAGHDTSSVLISFIMRLLS 314

Query: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           N+PTVYAAVLQEQEEIA+SK CGELL WEDLAKMKYTWR A+ETLR+  PIFGGFRK + 
Sbjct: 315 NDPTVYAAVLQEQEEIAKSKPCGELLTWEDLAKMKYTWRAAMETLRMFPPIFGGFRKTLK 374

Query: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420
           DI++ GYLIPKGWQIFW S +TH+D +IF++PSKFDP RF+N AS+PPYCF+ FG GPRI
Sbjct: 375 DIEYKGYLIPKGWQIFWASSMTHMDSSIFQDPSKFDPTRFENQASVPPYCFVAFGGGPRI 434

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFI-RDPMPTPTKGMPIKIWPR 472
           CPG EFAR+ETLVT+HYL+T+FTWKL   D+F  RDPMP PT+G+PI+I P+
Sbjct: 435 CPGYEFARIETLVTVHYLVTRFTWKLCCTDNFFSRDPMPVPTEGLPIEIVPK 486

[Close]

BLAST of CSPI03G17580 vs. NCBI nr
Match: XP_004140824.1 (cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010272 [Cucumis sativus])

HSP 1 Score: 968.0 bits (2501), Expect = 3.0e-278
Identity = 478/478 (100.00%), Postives = 478/478 (100.00%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60
           MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR
Sbjct: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240
           LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300

Query: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420
           DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 479
           CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 478

[Close]

BLAST of CSPI03G17580 vs. NCBI nr
Match: XP_008457111.1 (PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa] >TYK14034.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 931.0 bits (2405), Expect = 4.1e-267
Identity = 461/477 (96.65%), Postives = 466/477 (97.69%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60
           MIIYLTI FCLLIPIFLFLR SSKK+ LPPGSLGFP IGQSLSLLRAMRTNTAEQWAQQR
Sbjct: 1   MIIYLTILFCLLIPIFLFLRTSSKKQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQR 60

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL GE
Sbjct: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTGE 120

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180
           DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHI MHWH KQEVTVLPLMKTLTFNIVCSL
Sbjct: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSL 180

Query: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240
           LFGLEQG RRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE
Sbjct: 181 LFGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300
           LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLV+VAGFDTSSVLITFMMR+LA
Sbjct: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLA 300

Query: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN
Sbjct: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360

Query: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420
           DIQFGGYLIPKGWQIFWTSPVTHLDD IFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI
Sbjct: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMA 478
           CPGNEFARVETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKIWPR Q+FMA
Sbjct: 421 CPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR-QTFMA 476

[Close]

BLAST of CSPI03G17580 vs. NCBI nr
Match: XP_038899130.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida])

HSP 1 Score: 828.9 bits (2140), Expect = 2.2e-236
Identity = 420/484 (86.78%), Postives = 439/484 (90.70%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFL---RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWA 60
           MIIYL   F LLIPIFLFL   R+SSK   LPPGSLGFP IGQSLSLLRAMR NTAE+WA
Sbjct: 1   MIIYLITTFSLLIPIFLFLLTGRKSSK--NLPPGSLGFPFIGQSLSLLRAMRINTAEEWA 60

Query: 61  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 120
           QQR+R YGAISKLTLFGKPTVF+GGQSG+KLIFSGDC  +SNQQNESLRAILGE NLLEL
Sbjct: 61  QQRMRKYGAISKLTLFGKPTVFVGGQSGHKLIFSGDCG-ISNQQNESLRAILGECNLLEL 120

Query: 121 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIV 180
           IGEDHKRVRNALVSFLKPECLK+YVGKMD  IR HI+MHW  KQ+VTVLPLMKTLTFNIV
Sbjct: 121 IGEDHKRVRNALVSFLKPECLKQYVGKMD-XIRKHIKMHWQGKQQVTVLPLMKTLTFNIV 180

Query: 181 CSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEK 240
           CSLLFGLEQG RRERMIECFRVMI GVWSIPINLPFTRYNQSRRASR+IQEM+KELLDEK
Sbjct: 181 CSLLFGLEQGMRRERMIECFRVMIGGVWSIPINLPFTRYNQSRRASRRIQEMIKELLDEK 240

Query: 241 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMR 300
           RVELEEK  SSHQDLITCLLSI NEENEL+L+EKEIVHNIMLV+VAGFDTSSVLITF+MR
Sbjct: 241 RVELEEKRSSSHQDLITCLLSIGNEENELILTEKEIVHNIMLVMVAGFDTSSVLITFIMR 300

Query: 301 NLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRK 360
            LANNPTVY AVLQEQEEIARSKKCGELL WEDLAKMKYTWRVALETLRLVAPIFGGFRK
Sbjct: 301 FLANNPTVYVAVLQEQEEIARSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRK 360

Query: 361 AMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFG-- 420
           AMNDI+FGGYLIPKGWQIFW SPVTHLD TIFREP KFDP+RF NP SIPPYCF+GFG  
Sbjct: 361 AMNDIEFGGYLIPKGWQIFWASPVTHLDGTIFREPLKFDPNRFDNPTSIPPYCFVGFGGG 420

Query: 421 -----SGPRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWP 475
                 GPRICPGNEFARVETLVTIHYLIT+FTWKLLLDDHFIRDPMPTPTKGMPIKI P
Sbjct: 421 PRICPGGPRICPGNEFARVETLVTIHYLITRFTWKLLLDDHFIRDPMPTPTKGMPIKICP 480

[Close]

BLAST of CSPI03G17580 vs. NCBI nr
Match: XP_022139355.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia])

HSP 1 Score: 788.5 bits (2035), Expect = 3.3e-224
Identity = 391/477 (81.97%), Postives = 425/477 (89.10%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFL---RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWA 60
           M IYLTI F  LIPIFLFL   RRS   ++LPPGSLG P IGQSL LLRAMR+NTAEQW 
Sbjct: 1   MNIYLTI-FSFLIPIFLFLTRGRRSRSSQKLPPGSLGVPFIGQSLGLLRAMRSNTAEQWL 60

Query: 61  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 120
           Q+RI  YG ISKLTLFGKPTV +GGQ  NKLIF+GD A +SNQQNESLRAILGE NLLEL
Sbjct: 61  QRRITKYGPISKLTLFGKPTVLVGGQGANKLIFNGDTA-ISNQQNESLRAILGESNLLEL 120

Query: 121 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIV 180
            G+DHKRVR+ALV FLKPECLK+YVGKMDEE+R H+ MHW  KQEVTVLPLMKTLTFNIV
Sbjct: 121 TGDDHKRVRSALVXFLKPECLKQYVGKMDEEVRRHLEMHWQXKQEVTVLPLMKTLTFNIV 180

Query: 181 CSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEK 240
           CSLLFGLE+GTRRERMIECF+VMI GVWSIPINLPFTRYNQSR+AS KIQEMLK LLDEK
Sbjct: 181 CSLLFGLEEGTRRERMIECFQVMIGGVWSIPINLPFTRYNQSRQASIKIQEMLKNLLDEK 240

Query: 241 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMR 300
           RVELE KG S HQDLITCLLSI+N+E+E VL+EKEI+HNI+LV+VAG+DTSSVLITFM+R
Sbjct: 241 RVELEVKGTSPHQDLITCLLSIKNKEHEQVLTEKEIIHNIILVMVAGYDTSSVLITFMVR 300

Query: 301 NLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRK 360
            LANNPTVYAAVLQEQEEIARSK+CGELL WEDLAKMKYTWRVALETLRLVAP+FGGFRK
Sbjct: 301 FLANNPTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRK 360

Query: 361 AMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSG 420
           AM DI+FGGYLIPKGWQIFW SP+THLDDTIF EP KFDP+RF+N AS+P YCF+ FG G
Sbjct: 361 AMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGG 420

Query: 421 PRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQS 475
           PRICPG EFARVETLVTIHYLITQFTWKLLLDD FIRDPMP PTKG+PIK+ PR  S
Sbjct: 421 PRICPGYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETS 475

[Close]

BLAST of CSPI03G17580 vs. NCBI nr
Match: KAA8523694.1 (hypothetical protein F0562_010117 [Nyssa sinensis])

HSP 1 Score: 714.5 bits (1843), Expect = 6.0e-202
Identity = 333/472 (70.55%), Postives = 412/472 (87.29%), Query Frame = 0


Query: 1   MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQR 60
           M I+ TIF  LL   FL +RR S  ++LPPGSLG P+IGQSLSLL+AM+ NTAE+W ++R
Sbjct: 15  MNIFFTIFLFLLPIFFLLVRRRSSSQRLPPGSLGIPIIGQSLSLLQAMKANTAERWLEER 74

Query: 61  IRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGE 120
           IR YG+ISKL+LFGKPTVFI GQ+ NKL+F+GDC+V++NQQ +S+R ILG+RNLLEL GE
Sbjct: 75  IRKYGSISKLSLFGKPTVFIYGQAANKLVFTGDCSVITNQQAQSVRMILGDRNLLELSGE 134

Query: 121 DHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSL 180
           DHKRVR+ALVSFLKPECLK+YVGKM+EEIR H+ MHW  KQ+VTVLPLMKTLTFNI+CSL
Sbjct: 135 DHKRVRDALVSFLKPECLKQYVGKMEEEIREHLEMHWEGKQKVTVLPLMKTLTFNIICSL 194

Query: 181 LFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVE 240
           LFG+++G RR++++E F+ MI G+WS+P+NLPFTRYN+S RA+ ++Q MLK+L+ EKRVE
Sbjct: 195 LFGVQRGARRDKLVEYFQEMIQGMWSVPVNLPFTRYNRSLRATARVQNMLKDLIREKRVE 254

Query: 241 LEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLA 300
           LE++G SSHQDLITCLLSIR ++NE ++SEKEIVHN+MLV+VAG DTSSVLI+F+MR L+
Sbjct: 255 LEQQGASSHQDLITCLLSIRGKDNEELISEKEIVHNVMLVMVAGHDTSSVLISFIMRLLS 314

Query: 301 NNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMN 360
           N+PTVYAAVLQEQEEIA+SK CGELL WEDLAKMKYTWR A+ETLR+  PIFGGFRK + 
Sbjct: 315 NDPTVYAAVLQEQEEIAKSKPCGELLTWEDLAKMKYTWRAAMETLRMFPPIFGGFRKTLK 374

Query: 361 DIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRI 420
           DI++ GYLIPKGWQIFW S +TH+D +IF++PSKFDP RF+N AS+PPYCF+ FG GPRI
Sbjct: 375 DIEYKGYLIPKGWQIFWASSMTHMDSSIFQDPSKFDPTRFENQASVPPYCFVAFGGGPRI 434

Query: 421 CPGNEFARVETLVTIHYLITQFTWKLLLDDHFI-RDPMPTPTKGMPIKIWPR 472
           CPG EFAR+ETLVT+HYL+T+FTWKL   D+F  RDPMP PT+G+PI+I P+
Sbjct: 435 CPGYEFARIETLVTVHYLVTRFTWKLCCTDNFFSRDPMPVPTEGLPIEIVPK 486

[Close]

BLAST of CSPI03G17580 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 337.0 bits (863), Expect = 2.4e-92
Identity = 177/482 (36.72%), Postives = 289/482 (59.96%), Query Frame = 0


Query: 1   MIIYLTIFF-CLLIPIFLFLRRSSKK---RQLPPGSLGFPLIGQSLSLLRAMRTNTAEQW 60
           M I + +F   +L+ + L LR+         LPPG+ G PLIG+S S L A R    E++
Sbjct: 3   MAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKF 62

Query: 61  AQQRIRNYGAIS----KLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGER 120
              R+R + + S    K  LFG PT  + G SGNK +F+ +  +V +   +S+  I    
Sbjct: 63  ITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIF-PS 122

Query: 121 NLLELIGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTL 180
           ++     E+ +++R  L  F+KPE L+ YVG MDE  + H    W  + +V V PL K  
Sbjct: 123 SMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKF 182

Query: 181 TFNIVCSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKE 240
           TF+I C     +E   R  ++ E F  +  G++SIPI+LP TR+N++ +ASR +++ +  
Sbjct: 183 TFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSA 242

Query: 241 LLDEKRVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLI 300
           ++ +++ EL+        D+++ +L    E  +  L++K     I+ +L+ G DT+S++ 
Sbjct: 243 IVRQRKEELKAGKALEEHDILSHMLMNIGETKDEDLADK-----IIGLLIGGHDTASIVC 302

Query: 301 TFMMRNLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIF 360
           TF++  LA  P VY  VLQEQ+EI + KK  E L WED+ KM+Y+W VA E +R+V P+ 
Sbjct: 303 TFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLS 362

Query: 361 GGFRKAMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFL 420
           G FR+A++   F G+ IPKGW+++W++  TH++   F EP +F+P+RF+  +   PY ++
Sbjct: 363 GTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEG-SGPKPYTYV 422

Query: 421 GFGSGPRICPGNEFARVETLVTIHYLITQFTWKLLL--DDHFIRDPMPTPTKGMPIKIWP 473
            FG GPR+CPG E+AR+E L+ +H L+ +F W+ +   ++  + DP+P P KG+PI+I+P
Sbjct: 423 PFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFP 477

[Close]

BLAST of CSPI03G17580 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 308.9 bits (790), Expect = 7.1e-84
Identity = 164/468 (35.04%), Postives = 273/468 (58.33%), Query Frame = 0


Query: 6   TIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTA-EQWAQQRIRNY 65
           T+FF          ++  KK++L PG +G P IG+++   +A ++N   E +   RI  +
Sbjct: 23  TLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKH 82

Query: 66  GAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKR 125
           G I K  + G PT+ + G   N+LI S + ++V +    S   ++G   ++   GE H+ 
Sbjct: 83  GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRV 142

Query: 126 VRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSLLFGL 185
           +R  + + L    L+  + K+ + ++ H    W  K+E+++    K LTF +V   L+G+
Sbjct: 143 LRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYGI 202

Query: 186 EQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVELEEK 245
           +       M+E F  ++ GV+++P+  P +++ ++++A  +I+  L   + EKR E+E++
Sbjct: 203 KV---EIGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRREMEKE 262

Query: 246 GGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLANNPT 305
           G  + +   T    +  E  + V++E+E+V N++L++ A  DT+S  ++   + LA +PT
Sbjct: 263 G--AEKPNTTLFSRLVEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPT 322

Query: 306 VYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQF 365
               +LQE  +I  +K  GE L  ED+ KMKY+W+V  ET+RL  PIFG FRKA+ DI +
Sbjct: 323 CRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDY 382

Query: 366 GGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGN 425
           GGY IPKGW+I WT+  TH +  IF++P  FDP RF  P  I  Y +L FG GPR+C G+
Sbjct: 383 GGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDKP--IQAYTYLPFGGGPRLCAGH 442

Query: 426 EFARVETLVTIHYLITQFTWKLLLDDHFI-RDPMPTPTKGMPIKIWPR 472
           + A++  LV +H+++T F W L+  D  I  DP+P P+ GMPIKI P+
Sbjct: 443 QLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPK 483

[Close]

BLAST of CSPI03G17580 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 249.2 bits (635), Expect = 6.6e-66
Identity = 150/463 (32.40%), Postives = 246/463 (53.13%), Query Frame = 0


Query: 7   IFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQRIRNYGA 66
           +F CLL  I    R SS K  LPPG++G+P +G++  L     +     +   + R YG+
Sbjct: 15  LFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLY----SQDPNVFFAAKQRRYGS 74

Query: 67  ISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKRVR 126
           + K  + G P V I      K +      +       S   +LG++ +    G+ H ++R
Sbjct: 75  VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLR 134

Query: 127 NALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSLLFGLEQ 186
             ++    P+ ++  V  + E I       W   Q +     MKT TFN+    + G ++
Sbjct: 135 KLVLRAFMPDAIRNMVPHI-ESIAQESLNSWDGTQ-LNTYQEMKTYTFNVALISILGKDE 194

Query: 187 GTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVELEEKGG 246
              RE +  C+ ++  G  S+PINLP T ++++ +A +++ ++L  +L ++R     +  
Sbjct: 195 VYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRR-----QNP 254

Query: 247 SSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLANNPTVY 306
           SSH DL+   +     E++  L++++I  NI+ V+ A  DT++ ++T++++ LA+NPTV 
Sbjct: 255 SSHTDLLGSFM-----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVL 314

Query: 307 AAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGG 366
            AV +EQ  I + KK GE L WED  KM  T+RV  ETLR    +   FR+A+ D+++ G
Sbjct: 315 EAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEG 374

Query: 367 YLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEF 426
           YLIPKGW++       H +  IF +P KFDP RF+   +  P  F+ FGSG   CPGNE 
Sbjct: 375 YLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE--VAPKPNTFMPFGSGIHSCPGNEL 434

Query: 427 ARVETLVTIHYLITQFTWKLL-LDDHFIRDPMPTPTKGMPIKI 469
           A++E  V IH+L T++ W ++   D     P   P  G+PI +
Sbjct: 435 AKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459

[Close]

BLAST of CSPI03G17580 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 245.7 bits (626), Expect = 7.3e-65
Identity = 148/467 (31.69%), Postives = 247/467 (52.89%), Query Frame = 0


Query: 4   YLTIFFCLLIPIFLFLRRSSKKRQ-LPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQRIR 63
           +L +   +     L LRR+  +R  LPPGSLG PLIG++  L+ A +T   E +  +R+ 
Sbjct: 6   FLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVA 65

Query: 64  NYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGEDH 123
            YG++    LFG+PT+F      N+ +   +  +       S+  +LG+ +LL + G  H
Sbjct: 66  RYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLH 125

Query: 124 KRVRNALVSFLKPECLKEYVG-KMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSLL 183
           KR+ +  +SF     +K+++   +D  +R ++   W     V ++   K +TF +    L
Sbjct: 126 KRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLD-SW--SSRVLLMEEAKKITFELTVKQL 185

Query: 184 FGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVEL 243
              + G   E + + + ++I G +S+P+ L  T Y ++ +A RK+ E L  ++ ++R E 
Sbjct: 186 MSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE- 245

Query: 244 EEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLAN 303
           EE+G    +D++  LL+  +       S++EIV  ++ +LVAG++T+S ++T  ++ L  
Sbjct: 246 EEEGAERKKDMLAALLAADDG-----FSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTE 305

Query: 304 NPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMND 363
            P   A + +E E+I   K     L W D   M +T  V  ETLR+   I G FR+AM D
Sbjct: 306 TPLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTD 365

Query: 364 IQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPA--SIPPYCFLGFGSGPR 423
           ++  GY IPKGW++F +    HLD   F++   F+P R+++ +  + P   F  FG GPR
Sbjct: 366 VEIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPR 425

Query: 424 ICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPI 467
           +CPG E ARV   V +H L+T F+W     D  +  P     K  PI
Sbjct: 426 LCPGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPI 463

[Close]

BLAST of CSPI03G17580 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 243.8 bits (621), Expect = 2.8e-64
Identity = 146/470 (31.06%), Postives = 247/470 (52.55%), Query Frame = 0


Query: 3   IYLTIFFCLLIPIFLFL-----RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWA 62
           ++LT+F   L   FL       R  S K  LPPG++G+P +G++  L     +     + 
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY----SQDPNVFF 65

Query: 63  QQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLEL 122
           Q + + YG++ K  + G P V I      K +      +       S   +LG++ +   
Sbjct: 66  QSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFH 125

Query: 123 IGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIV 182
            G+ H ++R  ++    PE ++  V  ++   +  +R  W     +     MKT TFN+ 
Sbjct: 126 QGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQDSLR-SWEGTM-INTYQEMKTYTFNVA 185

Query: 183 CSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEK 242
              +FG ++   RE +  C+ ++  G  S+P+NLP T +++S +A +++ ++L  +L E+
Sbjct: 186 LLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSER 245

Query: 243 RVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMR 302
           R     + GSSH DL+   +  + E     L++++I  NI+ V+ A  DT++ +++++++
Sbjct: 246 R-----QNGSSHNDLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILK 305

Query: 303 NLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRK 362
            LA NP V  AV +EQ  I + K+ GE L W D  KM  T RV  ETLR+ + +   FR+
Sbjct: 306 YLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFRE 365

Query: 363 AMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSG 422
           A+ D+++ GYLIPKGW++       H    IF  P KFDP RF+   +  P  F+ FG+G
Sbjct: 366 AVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFE--VAPKPNTFMPFGNG 425

Query: 423 PRICPGNEFARVETLVTIHYLITQFTWKLL-LDDHFIRDPMPTPTKGMPI 467
              CPGNE A++E  + IH+L T+++W ++   D     P   P  G+PI
Sbjct: 426 THSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPI 457

The following BLAST results are available for this feature:

BLAST of CSPI03G17580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q50EK1	2.5e-102	40.93	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q50EK0	5.6e-102	40.42	Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1	[more]
A0A0S2II38	7.8e-96	37.97	Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=...	[more]
A0A0U2U8U5	8.7e-95	37.01	Beta-amyrin 28-monooxygenase OS=Barbarea vulgaris OX=50459 GN=CYP716A81 PE=1 SV=...	[more]
Q2MJ20	1.1e-94	38.85	Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV...	[more]

BLAST of CSPI03G17580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA02	1.5e-278	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1	[more]
A0A5A7TZG7	2.0e-267	96.65	Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C4V0	2.0e-267	96.65	cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1	[more]
A0A6J1CC35	1.6e-224	81.97	LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 G...	[more]
A0A5J5A0L0	2.9e-202	70.55	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_010117 PE=3 SV=1	[more]

BLAST of CSPI03G17580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140824.1	3.0e-278	100.00	cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010...	[more]
XP_008457111.1	4.1e-267	96.65	PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P4...	[more]
XP_038899130.1	2.2e-236	86.78	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida]	[more]
XP_022139355.1	3.3e-224	81.97	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia]	[more]
KAA8523694.1	6.0e-202	70.55	hypothetical protein F0562_010117 [Nyssa sinensis]	[more]

BLAST of CSPI03G17580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36110.1	2.4e-92	36.72	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT2G42850.1	7.1e-84	35.04	cytochrome P450, family 718	[more]
AT5G45340.1	6.6e-66	32.40	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT5G05690.1	7.3e-65	31.69	Cytochrome P450 superfamily protein	[more]
AT4G19230.1	2.8e-64	31.06	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 216..236
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 6..449 e-value: 1.5E-10 score: 38.4
None	No IPR available	PANTHER	PTHR24286:SF237	OS07G0520300 PROTEIN	coord: 4..471
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 4..471
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 378..402 score: 28.21 coord: 61..80 score: 25.21 coord: 292..318 score: 18.81 coord: 421..444 score: 27.13 coord: 172..190 score: 22.73 coord: 272..289 score: 26.65 coord: 411..421 score: 50.64
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 339..350 score: 34.88 coord: 283..300 score: 30.91 coord: 412..421 score: 54.7 coord: 421..432 score: 38.15
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 29..447 e-value: 1.3E-66 score: 225.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 19..469 e-value: 4.3E-116 score: 390.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 21..471
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 414..423

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G17580.1	CSPI03G17580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen