CSPI03G17100 (gene) Cucumber (PI 183967) v1

BLAST of CSPI03G17100 vs. ExPASy Swiss-Prot
Match: Q9AV23 (Silicon efflux transporter LSI3 OS=Oryza sativa subsp. japonica OX=39947 GN=LSI3 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.6e-148
Identity = 297/546 (54.40%), Postives = 391/546 (71.61%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MA+    KV++GS+AF VFW+LAVFP++PFLPIGRTAG++LGA+LM+VF V++ + AYA+
Sbjct: 1   MALASLPKVVMGSVAFGVFWMLAVFPSVPFLPIGRTAGALLGAVLMIVFHVISADDAYAS 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLF TMVV  YL+ A MF++LG++L+W+S+G +DL+CRVC+++A++SA FTND
Sbjct: 61  IDLPILGLLFATMVVGGYLKNAGMFRHLGRLLAWRSQGGRDLMCRVCVVTALASALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           T CVVLTEFVL++A + NLP +PFLLALA+SANIGSSATPIGNPQNLVIA  SKI F  F
Sbjct: 121 TCCVVLTEFVLELAAERNLPAKPFLLALATSANIGSSATPIGNPQNLVIAFNSKISFISF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           ++GILPAML G+ +N L++L MYWK L    D    SP+ +A    +        +   +
Sbjct: 181 LLGILPAMLAGMGINMLMLLCMYWKEL----DGGACSPDEVAAGKQME------AIEEGR 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
             +LN    +++ D  +A +P              +S ++++   + S M+E        
Sbjct: 241 RTALN----NNKKDDGDAATP--------------ASPEDDDGGDAESMMSE-------- 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
                N ST+ R                    +Q SE +       R++  KS  Y+VTV
Sbjct: 301 -----NISTKHR------------------WFMQCSEHR-------RKLFLKSFAYVVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVA ++GL+MSWTA+T A+ALVV+DF+DA+PCL+KVSYSLLVFF GMF+TV GFNKTG
Sbjct: 361 GMLVAYMLGLNMSWTAITTAIALVVVDFRDAEPCLDKVSYSLLVFFSGMFVTVSGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LP A WN M P+++I+ V+G  VL+++IL LSNLASNVPTVLL+G  VAA+AA ISP   
Sbjct: 421 LPGAIWNVMAPYSKINHVTGVTVLSVIILLLSNLASNVPTVLLMGDEVAAAAATISPAAV 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
            R+WLLLAW+STVAGNLSLLGSAANLIVCEQARR  + +Y+L+FWNH+ FGLPSTL+VTA
Sbjct: 481 TRSWLLLAWVSTVAGNLSLLGSAANLIVCEQARRATRNAYDLTFWNHVIFGLPSTLVVTA 480

Query: 541 IGLVLI 547
           IG+ LI
Sbjct: 541 IGIPLI 480

BLAST of CSPI03G17100 vs. ExPASy Swiss-Prot
Match: Q10SY9 (Silicon efflux transporter LSI2 OS=Oryza sativa subsp. japonica OX=39947 GN=LSI2 PE=1 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.9e-147
Identity = 294/539 (54.55%), Postives = 374/539 (69.39%), Query Frame = 0

Query: 8   KVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAAIDLPILG 67
           KV LGSIAFAVFW++AVFP++PFLPIGRTAGS+L A+LMV+F V++P+ AYA+IDLPILG
Sbjct: 9   KVALGSIAFAVFWMMAVFPSVPFLPIGRTAGSLLSAVLMVIFHVISPDDAYASIDLPILG 68

Query: 68  LLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTNDTSCVVLT 127
           LLF TMVV  YL  A MFK+LG++L+WKS+G +DL+CRVC+++A++SA FTNDT CVVLT
Sbjct: 69  LLFATMVVGSYLRNAGMFKHLGRLLAWKSQGGRDLMCRVCVVTALASALFTNDTCCVVLT 128

Query: 128 EFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQFVIGILPA 187
           EFVL++A + NLP +PFLLALASSANIGS+ATPIGNPQNLVIA  SKI F +F++GILPA
Sbjct: 129 EFVLELAAERNLPAKPFLLALASSANIGSAATPIGNPQNLVIAFNSKITFPKFLMGILPA 188

Query: 188 MLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQIPSLNSA 247
           MLVG+ VN +++L MYW+ L                                       A
Sbjct: 189 MLVGMAVNMVMLLCMYWRELG------------------------------------GGA 248

Query: 248 EWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDASKEWLPNA 307
           E       M A         VE  R+  S+K   ++  + +TM         S E   N 
Sbjct: 249 ELSVDGKQMEA---------VEEGRSPASAKSTPQLNGNGNTM--------MSLEMSENI 308

Query: 308 STQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTVGMLVALL 367
           +T                  K P  +Q +E +       R++  KS  Y+VTVGM+VA +
Sbjct: 309 TT------------------KHPWFMQCTEAR-------RKLFLKSFAYVVTVGMVVAYM 368

Query: 368 MGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGLPSAFWN 427
           +GL+MSWTA+T ALALVV+DF+DA+PCL+ VSYSLLVFF GMF+TV GFNKTGLP A W+
Sbjct: 369 VGLNMSWTAITTALALVVVDFRDAEPCLDTVSYSLLVFFSGMFITVSGFNKTGLPGAIWD 428

Query: 428 FMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEEKRAWLLL 487
           FM P+++++ V G  VL+++IL LSNLASNVPTVLL+G  VA +AA ISP     +WLLL
Sbjct: 429 FMAPYSKVNSVGGISVLSVIILLLSNLASNVPTVLLMGDEVAKAAALISPAAVTTSWLLL 469

Query: 488 AWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTAIGLVLI 547
           AW+STVAGNLSLLGSAANLIVCEQARR P+ +Y+L+FW H+ FG+PSTLIVTA+G+ LI
Sbjct: 489 AWVSTVAGNLSLLGSAANLIVCEQARRAPRNAYDLTFWQHIVFGVPSTLIVTAVGIPLI 469

BLAST of CSPI03G17100 vs. ExPASy Swiss-Prot
Match: Q54GU0 (Putative transporter arsB OS=Dictyostelium discoideum OX=44689 GN=arsB PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.7e-58
Identity = 165/527 (31.31%), Postives = 279/527 (52.94%), Query Frame = 0

Query: 31  LPIGRTAGSILGAMLMVVFRVLTPEQAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGK 90
           LPIGR   SI+GA LMV F ++ P++  + I+   + LL   M++S Y+E+A+++    K
Sbjct: 66  LPIGRAGSSIIGATLMVYFGIIQPKEIGSVINWDTIILLMSMMMLSNYMEQANIWGMASK 125

Query: 91  VLSWKSKGAKDLICRVCLISAISSAFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALAS 150
           +L WK K     + RVCLIS+I S+  TNDT CV LT  V+   +  NL   PFL+A+A+
Sbjct: 126 ILLWKCKSTSIFMVRVCLISSIMSSILTNDTVCVTLTPIVISACKSTNLTFFPFLMAIAT 185

Query: 151 SANIGSSATPIGNPQNLVIAVQSKIPFGQFVIGILPAMLVGVVVNALIILIMYWKLLSVQ 210
           SANIGSSA P+GNPQN++IA    + F  F    + + ++GV +N +++L+ + K L   
Sbjct: 186 SANIGSSALPVGNPQNMIIATAGGLNFFNFFKVSIVSSILGVCLNTILLLLYFKKDLKNL 245

Query: 211 KDE-----EDPSPEVIADEDVLSHRFSPARLSHSQIPSLNSAEWDSRLDLMNAQSPPCSN 270
                   E  +P+V   E++ ++        H    + N ++ +  ++ +N +     +
Sbjct: 246 NSNFNQLIETVNPKV---EEIDNNH-------HDDDGANNQSKNEKEMENINKEVEEEQH 305

Query: 271 TNVETIRNSVSSKDNEEIRRSHSTMTEPARIS--DASKEWLPNASTQKREEDFSSKSLNS 330
            N +   +  +   N      H+ +   + +   D S   + N   +K++E+F     NS
Sbjct: 306 NNDDDDDDGFNENKNNNNNGGHAILLVASSMDSIDLSDCSIIN-KDKKKKENFIEIYFNS 365

Query: 331 MEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTVGMLVALLMGLDMSWTAVTAALAL 390
            EK  E ++               ++ +K  V ++   +L+   +G+ M +T +     L
Sbjct: 366 KEKSIETIV------------NIIKLIFKFRVAIILTLVLIGFFIGMHMGFTVLFGVSIL 425

Query: 391 VVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGLPSAFWNFMEPHAQID----RVS 450
           ++ + KD    +  V + LL+FF G+F+ V+GF++     A W  +EP   ID     V 
Sbjct: 426 MICERKDITDIINSVDWELLLFFSGLFVLVEGFDRQFEKEA-WTILEPFVPIDSTHLNVL 485

Query: 451 GTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEEKRAWLLLAWISTVAGNLSL 510
              + +++IL L N+  NVP VL L  R+  + A          W+LLA++STVAGNL+L
Sbjct: 486 KIFIFSILILVLCNILGNVPLVLSLSPRLLEALA------PDFTWILLAFVSTVAGNLTL 545

Query: 511 LGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTAIGLVLI 547
           +GS ANLIV E+++      + + F  +LKFG+PST++V  IG+ ++
Sbjct: 546 VGSVANLIVAEKSKS----YHEIGFLEYLKFGVPSTILVILIGVPIV 558

BLAST of CSPI03G17100 vs. ExPASy Swiss-Prot
Match: Q8MIQ9 (P protein OS=Sus scrofa OX=9823 GN=Oca2 PE=2 SV=3)

HSP 1 Score: 88.2 bits (217), Expect = 3.1e-16
Identity = 136/568 (23.94%), Postives = 223/568 (39.26%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILG-----AMLMVVFRVLTPE 60
           MA  +    I   +  A   L  V+  I F  + RT  ++LG     A L V+    T  
Sbjct: 325 MAHQYLRASIEAQVTIAAVILAGVYVLIIFEIVHRTLAAMLGSLAALAALAVIGDRPTLT 384

Query: 61  QAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKG-AKDLICRVCLISAISS 120
           Q    ID   L LLFG M++         F Y        S+G    +I  +CLI+A+ S
Sbjct: 385 QVVEWIDFETLALLFGMMILVAIFSETGFFDYCAVKAYQLSRGRVWAMIIMLCLIAAVLS 444

Query: 121 AFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSK 180
           AF  N T+ ++ T   +++    NL PR  L+A     NIG +AT IG+P N++I    +
Sbjct: 445 AFLDNVTTALLFTPVTIRLCEVLNLDPRQVLIAEVIFTNIGGAATAIGDPPNVIIVSNQE 504

Query: 181 I-PFGQFVIGILPAMLVGVVVNAL----IILIMYWKLLSVQKDEEDPSPEVIADEDVLSH 240
           +   G    G    M  G+    L    ++ ++YW      K+     P  I +   L H
Sbjct: 505 LRKMGLDFAGFTAHMFAGICFVLLFSFPLLRLLYWNRKLYNKE-----PSEIVE---LKH 564

Query: 241 RFSPARLSHSQIPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHST 300
                RL+  +I   +  E   R  L+                  V S +    RR HS 
Sbjct: 565 EIHVWRLTAQRISPASREETAVRGLLL----------------EKVLSLERLLARRLHSF 624

Query: 301 MTEPARISDASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRI 360
             +   IS   K W  N    +++   S ++L +                          
Sbjct: 625 HRQ---ISQEDKNWETNIQELQKKHRISDRTLLT-------------------------- 684

Query: 361 AWKSCVYLVTVGMLVALL------MGLDMSWTAVTAALALVVL-DFKDAQPCLEKVSYSL 420
               CV ++ + + +  L      + LD+ W A+  A+ L++L D  D +  L +V ++ 
Sbjct: 685 ---KCVTVLGLVIFMFFLNSFVPGVHLDLGWIAILGAIWLLILADIHDFEIILHRVEWAT 744

Query: 421 LVFFCGMFMTVDG------FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLAS 480
           L+FF  +F+ ++           G  +A    M P  Q    +  +V+  V    S+L  
Sbjct: 745 LLFFAALFILMEALAHLHLIEYVGEQTALLIKMVPEDQ-RLAAAIIVVVWVSAIASSLID 804

Query: 481 NVP-TVLLLGARVAASAAAISPTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRT 540
           N+P T  ++   +  S               LA  + + GN +L+G++AN +VC  A   
Sbjct: 805 NIPFTATMIPVLLNLSRDPEISLPAPPLMYALALGACLGGNGTLIGASAN-VVC--AGIA 832

Query: 541 PQLSYNLSFWNHLKFGLPSTLIVTAIGL 544
            Q  Y  SF    + G P  ++   +G+
Sbjct: 865 EQHGYGFSFMEFFRLGFPMMVVSCMVGM 832

BLAST of CSPI03G17100 vs. ExPASy Swiss-Prot
Match: Q04671 (P protein OS=Homo sapiens OX=9606 GN=OCA2 PE=2 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 1.6e-15
Identity = 134/556 (24.10%), Postives = 228/556 (41.01%), Query Frame = 0

Query: 14  IAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA-----IDLPILGL 73
           +  A   L  V+  I F  + RT  ++LG++  +    +  ++         ID   L L
Sbjct: 331 VTIATAILAGVYALIIFEIVHRTLAAMLGSLAALAALAVIGDRPSLTHVVEWIDFETLAL 390

Query: 74  LFGTMVVSVYLERADMFKYLGKVLSWKSKG-AKDLICRVCLISAISSAFFTNDTSCVVLT 133
           LFG M++         F Y        S+G    +I  +CLI+A+ SAF  N T+ ++ T
Sbjct: 391 LFGMMILVAIFSETGFFDYCAVKAYRLSRGRVWAMIIMLCLIAAVLSAFLDNVTTMLLFT 450

Query: 134 EFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI-PFGQFVIGILP 193
              +++    NL PR  L+A     NIG +AT IG+P N++I    ++   G    G   
Sbjct: 451 PVTIRLCEVLNLDPRQVLIAEVIFTNIGGAATAIGDPPNVIIVSNQELRKMGLDFAGFTA 510

Query: 194 AMLVGVVVNALI----ILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQIP 253
            M +G+ +  L+    + ++YW      K+     P  I +   L H     RL+  +I 
Sbjct: 511 HMFIGICLVLLVCFPLLRLLYWNRKLYNKE-----PSEIVE---LKHEIHVWRLTAQRIS 570

Query: 254 SLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDASKE 313
             +  E   R  L+                  V + ++   RR H   T   +IS   K 
Sbjct: 571 PASREETAVRRLLL----------------GKVLALEHLLARRLH---TFHRQISQEDKN 630

Query: 314 WLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCV----YLV 373
           W                  N  E QK+  I   S+G          I    C+    +++
Sbjct: 631 W----------------ETNIQELQKKHRI---SDG----------ILLAKCLTVLGFVI 690

Query: 374 TVGMLVALLMG--LDMSWTAVTAALALVVL-DFKDAQPCLEKVSYSLLVFFCGMFMTVDG 433
            +  L + + G  LD+ W A+  A+ L++L D  D +  L +V ++ L+FF  +F+ ++ 
Sbjct: 691 FMFFLNSFVPGIHLDLGWIAILGAIWLLILADIHDFEIILHRVEWATLLFFAALFVLMEA 750

Query: 434 ------FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYL-SNLASNVP-TVLLLGAR 493
                     G  +A    M P  Q  R+   +VL + +  L S+L  N+P T  ++   
Sbjct: 751 LAHLHLIEYVGEQTALLIKMVPEEQ--RLIAAIVLVVWVSALASSLIDNIPFTATMIPVL 810

Query: 494 VAASAAAISPTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNH 544
           +  S               LA+ + + GN +L+G++AN +VC  A    Q  Y  SF   
Sbjct: 811 LNLSHDPEVGLPAPPLMYALAFGACLGGNGTLIGASAN-VVC--AGIAEQHGYGFSFMEF 825

BLAST of CSPI03G17100 vs. ExPASy TrEMBL
Match: A0A1L7B7J1 (LSi2 OS=Cucumis sativus var. sativus OX=869827 GN=LSi2 PE=2 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 1.3e-299
Identity = 546/547 (99.82%), Postives = 546/547 (99.82%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI FGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV
Sbjct: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLIK
Sbjct: 541 IGLVLIK 547

BLAST of CSPI03G17100 vs. ExPASy TrEMBL
Match: A0A0A0L6L7 (CitMHS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G182780 PE=4 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 1.3e-299
Identity = 546/547 (99.82%), Postives = 546/547 (99.82%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI FGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV
Sbjct: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLIK
Sbjct: 541 IGLVLIK 547

BLAST of CSPI03G17100 vs. ExPASy TrEMBL
Match: A0A1S4DT02 (putative transporter arsB OS=Cucumis melo OX=3656 GN=LOC103484097 PE=4 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.1e-293
Identity = 533/547 (97.44%), Postives = 540/547 (98.72%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMV+FRVLTPEQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVIFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILI+YWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNSAEWDSRLD MNAQSPPCSN NVETIRNSVSSKDN EI RSHST+TEPARISDAS
Sbjct: 241 IPSLNSAEWDSRLDSMNAQSPPCSNANVETIRNSVSSKDN-EIHRSHSTLTEPARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNAS QKREE FSSKSLNSM+KQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV
Sbjct: 301 KEWLPNASPQKREEGFSSKSLNSMDKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GML+ALL+GLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLIALLLGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP EE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPAEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLI+
Sbjct: 541 IGLVLIR 546

BLAST of CSPI03G17100 vs. ExPASy TrEMBL
Match: A0A5D3DH83 (Putative transporter arsB OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00790 PE=4 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.1e-293
Identity = 533/547 (97.44%), Postives = 540/547 (98.72%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMV+FRVLTPEQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVIFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILI+YWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNSAEWDSRLD MNAQSPPCSN NVETIRNSVSSKDN EI RSHST+TEPARISDAS
Sbjct: 241 IPSLNSAEWDSRLDSMNAQSPPCSNANVETIRNSVSSKDN-EIHRSHSTLTEPARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNAS QKREE FSSKSLNSM+KQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV
Sbjct: 301 KEWLPNASPQKREEGFSSKSLNSMDKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GML+ALL+GLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLIALLLGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP EE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPAEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLI+
Sbjct: 541 IGLVLIR 546

BLAST of CSPI03G17100 vs. ExPASy TrEMBL
Match: A0A6J1CIT4 (silicon efflux transporter LSI2 OS=Momordica charantia OX=3673 GN=LOC111011742 PE=4 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 5.2e-272
Identity = 497/552 (90.04%), Postives = 524/552 (94.93%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFA+FWLLAVFPAIPFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVIFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKS+GAKDLICRVC+ISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSRGAKDLICRVCVISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNA+IIL++YWKLLSVQKDEED   EV+ADEDV SHRFSPARLSH+ 
Sbjct: 181 VIGILPAMLVGIVVNAVIILVLYWKLLSVQKDEEDAPSEVVADEDVNSHRFSPARLSHTH 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNT---NVETIRNSVSSKDNEEIRRSHSTMTEPARIS 300
           IPS+NS EW+SRLD MNAQS PC+N    NVET+RNS+SSKDN EI RS S MTE AR+S
Sbjct: 241 IPSINSPEWNSRLDSMNAQSSPCANANIENVETVRNSISSKDN-EIHRSLSAMTESARVS 300

Query: 301 DASKEWLPNASTQKREEDFSSKSLNSMEKQKE--PVILQSSEGKEHWSTKWRRIAWKSCV 360
           DASKEWLP +S QKREE F SKS NSM+KQKE   V LQSSEGKEHWSTKWRRIAWK CV
Sbjct: 301 DASKEWLPTSSPQKREESFPSKSFNSMDKQKELVSVSLQSSEGKEHWSTKWRRIAWKCCV 360

Query: 361 YLVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420
           YLVT+GMLVALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG
Sbjct: 361 YLVTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420

Query: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAI 480
           FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAI
Sbjct: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAI 480

Query: 481 SPTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPST 540
           SP +EKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFG+PST
Sbjct: 481 SPDQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGVPST 540

Query: 541 LIVTAIGLVLIK 548
           L+VTAIGLVLI+
Sbjct: 541 LLVTAIGLVLIR 551

BLAST of CSPI03G17100 vs. NCBI nr
Match: XP_004140769.1 (silicon efflux transporter LSI3 [Cucumis sativus] >XP_011651172.1 silicon efflux transporter LSI3 [Cucumis sativus] >XP_011651173.1 silicon efflux transporter LSI3 [Cucumis sativus] >XP_031738919.1 silicon efflux transporter LSI3 [Cucumis sativus] >APT69295.1 LSi2 [Cucumis sativus var. sativus] >KGN57393.1 hypothetical protein Csa_011781 [Cucumis sativus])

HSP 1 Score: 1038.1 bits (2683), Expect = 2.7e-299
Identity = 546/547 (99.82%), Postives = 546/547 (99.82%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI FGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV
Sbjct: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLIK
Sbjct: 541 IGLVLIK 547

BLAST of CSPI03G17100 vs. NCBI nr
Match: XP_016899111.1 (PREDICTED: putative transporter arsB [Cucumis melo] >XP_016899112.1 PREDICTED: putative transporter arsB [Cucumis melo] >XP_016899113.1 PREDICTED: putative transporter arsB [Cucumis melo] >XP_016899114.1 PREDICTED: putative transporter arsB [Cucumis melo] >KAA0033676.1 putative transporter arsB [Cucumis melo var. makuwa] >TYK22974.1 putative transporter arsB [Cucumis melo var. makuwa])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.9e-292
Identity = 533/547 (97.44%), Postives = 540/547 (98.72%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMV+FRVLTPEQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVIFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILI+YWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNSAEWDSRLD MNAQSPPCSN NVETIRNSVSSKDN EI RSHST+TEPARISDAS
Sbjct: 241 IPSLNSAEWDSRLDSMNAQSPPCSNANVETIRNSVSSKDN-EIHRSHSTLTEPARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNAS QKREE FSSKSLNSM+KQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV
Sbjct: 301 KEWLPNASPQKREEGFSSKSLNSMDKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GML+ALL+GLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLIALLLGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP EE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPAEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLI+
Sbjct: 541 IGLVLIR 546

BLAST of CSPI03G17100 vs. NCBI nr
Match: XP_038903236.1 (silicon efflux transporter LSI2 [Benincasa hispida] >XP_038903243.1 silicon efflux transporter LSI2 [Benincasa hispida] >XP_038903252.1 silicon efflux transporter LSI2 [Benincasa hispida])

HSP 1 Score: 996.9 bits (2576), Expect = 6.9e-287
Identity = 521/547 (95.25%), Postives = 536/547 (97.99%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGS+LGAMLMVVFRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILI+YWKLLSVQKDEEDPSPE++ADEDVLSHRFSPARLSH+Q
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEIVADEDVLSHRFSPARLSHTQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPS+NSAEWDSRLDLMNAQS PCSN NVET+RNSVSSKDN EI RSHS MTE ARISDAS
Sbjct: 241 IPSINSAEWDSRLDLMNAQSSPCSNANVETLRNSVSSKDN-EIHRSHSAMTESARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNAS QKREE FSSKS+NSMEKQKEPV LQSSEGKEHWSTKWRRIAWK CVYLVT+
Sbjct: 301 KEWLPNASPQKREEGFSSKSVNSMEKQKEPVSLQSSEGKEHWSTKWRRIAWKCCVYLVTI 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GML+ALL+GL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLIALLLGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS TEE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISATEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFG+PSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLI+
Sbjct: 541 IGLVLIR 546

BLAST of CSPI03G17100 vs. NCBI nr
Match: XP_022141316.1 (silicon efflux transporter LSI2 [Momordica charantia] >XP_022141318.1 silicon efflux transporter LSI2 [Momordica charantia] >XP_022141319.1 silicon efflux transporter LSI2 [Momordica charantia] >XP_022141320.1 silicon efflux transporter LSI2 [Momordica charantia])

HSP 1 Score: 946.4 bits (2445), Expect = 1.1e-271
Identity = 497/552 (90.04%), Postives = 524/552 (94.93%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFA+FWLLAVFPAIPFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVIFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKS+GAKDLICRVC+ISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSRGAKDLICRVCVISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNA+IIL++YWKLLSVQKDEED   EV+ADEDV SHRFSPARLSH+ 
Sbjct: 181 VIGILPAMLVGIVVNAVIILVLYWKLLSVQKDEEDAPSEVVADEDVNSHRFSPARLSHTH 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNT---NVETIRNSVSSKDNEEIRRSHSTMTEPARIS 300
           IPS+NS EW+SRLD MNAQS PC+N    NVET+RNS+SSKDN EI RS S MTE AR+S
Sbjct: 241 IPSINSPEWNSRLDSMNAQSSPCANANIENVETVRNSISSKDN-EIHRSLSAMTESARVS 300

Query: 301 DASKEWLPNASTQKREEDFSSKSLNSMEKQKE--PVILQSSEGKEHWSTKWRRIAWKSCV 360
           DASKEWLP +S QKREE F SKS NSM+KQKE   V LQSSEGKEHWSTKWRRIAWK CV
Sbjct: 301 DASKEWLPTSSPQKREESFPSKSFNSMDKQKELVSVSLQSSEGKEHWSTKWRRIAWKCCV 360

Query: 361 YLVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420
           YLVT+GMLVALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG
Sbjct: 361 YLVTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDG 420

Query: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAI 480
           FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAI
Sbjct: 421 FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAI 480

Query: 481 SPTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPST 540
           SP +EKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR+PQLSYNLSFWNHLKFG+PST
Sbjct: 481 SPDQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRSPQLSYNLSFWNHLKFGVPST 540

Query: 541 LIVTAIGLVLIK 548
           L+VTAIGLVLI+
Sbjct: 541 LLVTAIGLVLIR 551

BLAST of CSPI03G17100 vs. NCBI nr
Match: XP_023541235.1 (silicon efflux transporter LSI2-like [Cucurbita pepo subsp. pepo] >XP_023541236.1 silicon efflux transporter LSI2-like [Cucurbita pepo subsp. pepo] >XP_023541237.1 silicon efflux transporter LSI2-like [Cucurbita pepo subsp. pepo] >XP_023541238.1 silicon efflux transporter LSI2-like [Cucurbita pepo subsp. pepo] >XP_023541239.1 silicon efflux transporter LSI2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 937.9 bits (2423), Expect = 3.8e-269
Identity = 497/550 (90.36%), Postives = 519/550 (94.36%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAF +FWLLAVFPAIPFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFGIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVLFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIA+QHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQS IPFGQF
Sbjct: 121 TSCVVLTEFVLKIAKQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSGIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILI YWKLLS+QKDEED  PE++ADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGIVVNALIILISYWKLLSIQKDEEDVPPEIVADEDVNSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEP-ARISDA 300
           IPSLNS EWDSRLD M AQSPP SN NVET+R SVSSKDN EI  SH  +TE  ARISDA
Sbjct: 241 IPSLNSQEWDSRLDSMYAQSPPNSNANVETLRTSVSSKDN-EIHWSHGAVTEAVARISDA 300

Query: 301 SKEWLPNAS--TQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYL 360
           SKE +PNAS   QKREE FSSKS NSM+KQKEP+ LQ SEGK+HWSTKWRR+AWK CVYL
Sbjct: 301 SKECVPNASPPPQKREEGFSSKSFNSMDKQKEPLPLQVSEGKQHWSTKWRRMAWKCCVYL 360

Query: 361 VTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFN 420
           VT+GMLVALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMF+TVDGFN
Sbjct: 361 VTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFITVDGFN 420

Query: 421 KTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP 480
           KTGLP+AFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP
Sbjct: 421 KTGLPNAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP 480

Query: 481 TEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI 540
            +EKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI
Sbjct: 481 AQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI 540

Query: 541 VTAIGLVLIK 548
           +T IGLVLIK
Sbjct: 541 ITGIGLVLIK 549

BLAST of CSPI03G17100 vs. TAIR 10
Match: AT1G02260.1 (Divalent ion symporter )

HSP 1 Score: 646.4 bits (1666), Expect = 2.1e-185
Identity = 354/551 (64.25%), Postives = 426/551 (77.31%), Query Frame = 0

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAM   +K++LGS+AFA+FW+LAVFP++PFLPIGRTAGS+ GAMLMV+F+V+TPEQAYAA
Sbjct: 1   MAMAPVIKLVLGSVAFAIFWILAVFPSVPFLPIGRTAGSLFGAMLMVIFQVITPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVS+YLERADMFKYLG +LSWKS+G KDL+CRVCL+SA+SSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSIYLERADMFKYLGTLLSWKSRGPKDLLCRVCLVSAVSSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQ NLPP PFLLALA+SANIGSSATPIGNPQNLVIAVQSKIPF +F
Sbjct: 121 TSCVVLTEFVLKIARQKNLPPHPFLLALATSANIGSSATPIGNPQNLVIAVQSKIPFWEF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEED----PSPEVIADEDVLSHRFSPARL 240
           ++G+ PAM+VG+ VNA+++L MYW+LLS  K++E+       EV+A EDV SHRFSPA  
Sbjct: 181 LLGVFPAMIVGITVNAMLLLGMYWRLLSDHKEDEEEVQNADSEVVAQEDVQSHRFSPATF 240

Query: 241 SHSQIPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARI 300
           S      ++S + + R+D              ET+RN   S    E+   +         
Sbjct: 241 S-----PVSSEDSNLRMDA------------AETLRNRAGSAGESELISCN--------- 300

Query: 301 SDASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVY 360
           S+AS+E   +A +Q       S + N+M + K                +WRR+ WKS VY
Sbjct: 301 SNASREQHNDAESQG-----ESNNTNNMFQTK----------------RWRRVLWKSSVY 360

Query: 361 LVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420
            +T+GML++LLMGL+MSWTA+TAALALVVLDFKDA+P LEKVSYSLL+FFCGMF+TVDGF
Sbjct: 361 FITLGMLISLLMGLNMSWTAITAALALVVLDFKDARPSLEKVSYSLLIFFCGMFITVDGF 420

Query: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS 480
           NKTG+P+A W+ MEP+A+ID+  G  VLA+VIL LSN+ASNVPTVLLLGARVAAS  A+ 
Sbjct: 421 NKTGIPTALWDLMEPYAKIDQAKGIAVLAVVILVLSNVASNVPTVLLLGARVAAS--AMG 480

Query: 481 PTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTL 540
             EEK+AWLLLAW+STVAGNL+LLGSAANLIVCEQARR     Y L+F  H KFGLPSTL
Sbjct: 481 REEEKKAWLLLAWVSTVAGNLTLLGSAANLIVCEQARRAVSHGYTLTFTKHFKFGLPSTL 502

Query: 541 IVTAIGLVLIK 548
           IVTAIGL LIK
Sbjct: 541 IVTAIGLFLIK 502