CSPI03G16250 (gene) Cucumber (PI 183967) v1

BLAST of CSPI03G16250 vs. ExPASy Swiss-Prot
Match: Q8VYR9 (K(+) efflux antiporter 5 OS=Arabidopsis thaliana OX=3702 GN=KEA5 PE=2 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 3.2e-245
Identity = 449/559 (80.32%), Postives = 514/559 (91.95%), Query Frame = 0

Query: 21  LVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDKVLEKEFSDNDLPEG 80
           L  L+     ++ARSD+E RERFYGN++NSTAP +G+GS A+MFD+VLEKEFS+ND PEG
Sbjct: 9   LTFLLLLGTSLSARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFSENDSPEG 68

Query: 81  SGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQLQDVFSLENEESDDVTT 140
           S G+SFNSSVADQ+AE+ETVAK+THEKGK+ND+Q+ NGTR FQLQDVFSLENE+SDD+ T
Sbjct: 69  SDGASFNSSVADQQAEIETVAKVTHEKGKRNDTQENNGTRPFQLQDVFSLENEDSDDM-T 128

Query: 141 LIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCLGQPVIVGYLLAG 200
           LIDKK+NVFVMSNKKSKYP+LQVDLRLISDLVV+IV AAIGGI+FSCLGQPVIVGYLLAG
Sbjct: 129 LIDKKNNVFVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCLGQPVIVGYLLAG 188

Query: 201 SIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVAIFGGFLQIIIFM 260
           SIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFS+TKLKVVG VA+ GG LQI++ M
Sbjct: 189 SIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVAVLGGLLQIVLLM 248

Query: 261 FLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQVTIGTLILQDCA 320
           FLCG+ A+L GA+LSEG+FVG+FLSMSSTAVVVKFLVERNS+++LHGQVTIG LI QDC 
Sbjct: 249 FLCGVTALLCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQVTIGILIFQDCV 308

Query: 321 VGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFVPRFLKLMMQLSSQT 380
           VGLLFALLPVLGG++GL+ G+ISMGKLLL+LS+YLT AS+L+WSFVPRFLKLM+QLSSQT
Sbjct: 309 VGLLFALLPVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPRFLKLMIQLSSQT 368

Query: 381 NELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHTLDQVEPIRNLFAAL 440
           NELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVM+STT+F QHTL+QVEPIRNLFAAL
Sbjct: 369 NELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLEQVEPIRNLFAAL 428

Query: 441 FLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYGIRTSFQVGVMLAQI 500
           FLSSIGMLI+VHFLW+H+DILLASV+LV+ +KTA+A +V KAF Y +R SF VGV+LAQI
Sbjct: 429 FLSSIGMLINVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMRISFHVGVLLAQI 488

Query: 501 GEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAVLNLGVLMHWFPSEN 560
           GEFAFVLLSRASNLH+I GK+YLLLLGTTALSLVTTPLLFKLIP+ +NLGVL+ WFPSEN
Sbjct: 489 GEFAFVLLSRASNLHVIEGKMYLLLLGTTALSLVTTPLLFKLIPSAMNLGVLLRWFPSEN 548

Query: 561 NIQNE---EKVSMIEAHNR 577
           +  NE   EK S+IE HNR
Sbjct: 549 SSPNESLQEKASLIEVHNR 566

BLAST of CSPI03G16250 vs. ExPASy Swiss-Prot
Match: B5X0N6 (K(+) efflux antiporter 6 OS=Arabidopsis thaliana OX=3702 GN=KEA6 PE=2 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 2.1e-188
Identity = 372/586 (63.48%), Postives = 458/586 (78.16%), Query Frame = 0

Query: 9   RRGGAIGSFLCILVVLIS-----SHIHVTARSDKEIRERFYGNLINSTAPTSG------- 68
           RR       L +L++L+S     S     A SD ++ +    N  +S A  +        
Sbjct: 8   RRFSLSSQQLALLLLLLSFFLCFSVASPRAISDSDLLDETVANSSSSVASLNASSSSSLV 67

Query: 69  ---DGSFAQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDS 128
              +GSFA + D+ LEKEF+++D  E +   SFN+SVA Q+A LETVA++  +  KKN++
Sbjct: 68  KPKEGSFADIIDRALEKEFNESDQNEVADPGSFNNSVAGQQAVLETVARV--KSTKKNET 127

Query: 129 QKANGTRAFQLQDVFSLENE-ESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLV 188
           ++    + FQL DVF+L N+  ++D  TLID+KDNVF++SN KSKYPVLQ+DLRLISDLV
Sbjct: 128 KE---EKRFQLHDVFNLNNDNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLV 187

Query: 189 VVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFAL 248
           VVIVSA  GGI F+C GQPVI GYLLAGSIIGPGGL FISEMVQVETVAQFGVVFLLFAL
Sbjct: 188 VVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFAL 247

Query: 249 GLEFSLTKLKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVV 308
           GLEFS  KLKVV +VA+ GG LQI++FMFLCGI   L G K SEGVFVG+FLSMSSTAVV
Sbjct: 248 GLEFSTAKLKVVRSVAVLGGLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVV 307

Query: 309 VKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLS 368
           +KFL+E+NS+N+LHGQVTIG LILQDCAVGLLFALLPVL G++G++ GM+S+GK++++L 
Sbjct: 308 LKFLMEKNSTNSLHGQVTIGILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLL 367

Query: 369 VYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFV 428
            +L   SILS + +P  LKLM+ LSSQTNELYQLAAVAFCLL AWCSDKLGLSLELGSF 
Sbjct: 368 SFLAVLSILSRTCIPWLLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFA 427

Query: 429 AGVMVSTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVK 488
           AGVM+STTD  +HTL+Q+EPIRNLFAALFL+SIGML++VHFLW+H+DILLASV+LV+ +K
Sbjct: 428 AGVMISTTDLAEHTLEQIEPIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIK 487

Query: 489 TAVATIVAKAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALS 548
           T + T V K FGY  +T+  VG+ LAQIGEFAFVLLSRASNLHLI GK+YLLLLGTTALS
Sbjct: 488 TTIVTTVVKGFGYNNKTALLVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALS 547

Query: 549 LVTTPLLFKLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           LVTTPL+FK+IPAV++LG+L+ WF  ++ I+  E V       RM+
Sbjct: 548 LVTTPLVFKMIPAVVHLGILLKWFSPDSTIEKGEIVRSESGKQRMI 588

BLAST of CSPI03G16250 vs. ExPASy Swiss-Prot
Match: Q9ZUN3 (K(+) efflux antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=KEA4 PE=2 SV=2)

HSP 1 Score: 644.8 bits (1662), Expect = 9.2e-184
Identity = 361/532 (67.86%), Postives = 427/532 (80.26%), Query Frame = 0

Query: 35  SDKEIRERFYGNLI--NSTAPTSGDGSFAQMFDKVLEKEFSDNDLPEGSGGSSFNSSVAD 94
           +D  +     G ++  N+T+    + SFA M D+ LEKEF DND  E     SFN+SVAD
Sbjct: 43  TDSVVTREINGTVVESNATSAKPREDSFADMIDRALEKEFPDNDQNEVPDPGSFNNSVAD 102

Query: 95  QEAELETVAKITHEKGKKNDSQKANGTRAFQLQDVFSLENEES-DDVTTLIDKKDNVFVM 154
           Q+A LETVA++   K KKN+      T+  + +  F+L+NE   +D   LID+KDNVF+M
Sbjct: 103 QQAVLETVARV---KPKKNE------TKTKEEKSFFNLDNENGVEDTPRLIDRKDNVFIM 162

Query: 155 SNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFI 214
           SN KSKYPVLQ+DLRLISDLVVVIVSA  GGI F+C GQPVI GYLLAGSIIGPGGL F+
Sbjct: 163 SNPKSKYPVLQLDLRLISDLVVVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGLSFV 222

Query: 215 SEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVAIFGGFLQIIIFMFLCGIVAMLSG 274
           SEMVQVETVAQFGV+FLLFALGLEFS  KL+VV AVAI GG LQI +FM L GI A L G
Sbjct: 223 SEMVQVETVAQFGVIFLLFALGLEFSAAKLRVVRAVAIPGGLLQIFLFMCLSGITASLCG 282

Query: 275 AKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVL 334
            KL+EG+FVG+FLSMSSTAVV+KFL+ERNS + LHGQ+T+GTLILQDCAVGLLFALLPVL
Sbjct: 283 GKLTEGIFVGAFLSMSSTAVVLKFLMERNSISALHGQITVGTLILQDCAVGLLFALLPVL 342

Query: 335 GGHNGLILGMISMGKLLLVLSVYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAF 394
           GG +G++ G++SM K L +L  +L A  +LS ++VP FLKLM  LSSQTNELYQLAAVAF
Sbjct: 343 GGTSGVLQGVLSMAKSLAILIAFLGALFVLSRTWVPWFLKLMTSLSSQTNELYQLAAVAF 402

Query: 395 CLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHV 454
           CLL AWCSDKLGLSLELGSF AGVM+STTD  QHTL+QVEPIRN FAALFL+SIGMLIH+
Sbjct: 403 CLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIHM 462

Query: 455 HFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYGIRTSFQVGVMLAQIGEFAFVLLSRA 514
           HFLW+H+DILLA+V+LV+ +KT V  IV K FGY  +T+  VG+ LAQIGEFAFVLLSRA
Sbjct: 463 HFLWNHVDILLAAVLLVIVIKTVVVAIVVKVFGYNNKTAVLVGMSLAQIGEFAFVLLSRA 522

Query: 515 SNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAVLNLGVLMHWFPSENNIQ 564
           SNLHLI  K+YLLLLGTTALSLVTTPLLFKLIPAV++LGVL+ WF  +++ +
Sbjct: 523 SNLHLIESKLYLLLLGTTALSLVTTPLLFKLIPAVVHLGVLLRWFSPDSSTE 565

BLAST of CSPI03G16250 vs. ExPASy Swiss-Prot
Match: A8AQP0 (Glutathione-regulated potassium-efflux system protein KefB OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) OX=290338 GN=kefB PE=3 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.5e-37
Identity = 134/380 (35.26%), Postives = 211/380 (55.53%), Query Frame = 0

Query: 169 SDLV---VVIVSAAIGGI-IFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFG 228
           SDL+   V+ + AA+  + + S LG   ++GYLLAG  IGP GL FIS++ ++   ++ G
Sbjct: 4   SDLLTAGVMFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELG 63

Query: 229 VVFLLFALGLEFSLTKLKVVGAVAIFG-GFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSF 288
           VVFL+F +GLE + +KL  +   +IFG G  Q+++   +   + ML+       V  G  
Sbjct: 64  VVFLMFIIGLELNPSKLWQLRR-SIFGVGAAQVMLSAVVLAGLLMLTDFSWQAAVIGGIG 123

Query: 289 LSMSSTAVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMIS 348
           L+MSSTA+ ++ + E+  + +  GQ+    L+ QD AV    AL+P+L G        I 
Sbjct: 124 LAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDWIK 183

Query: 349 MGKLLLVLSVYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLG 408
           +G  +L  +  L     L    V RF+       S   E++  A +   L SA   D LG
Sbjct: 184 VGMKVLAFAGMLIGGRYLLRP-VFRFI-----ADSGVREVFTAATLLLVLGSALFMDALG 243

Query: 409 LSLELGSFVAGVMVSTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLA 468
           LS+ LG+F+AGV+++ +++       ++P + L   LF  S+GM +++  L++HL  +  
Sbjct: 244 LSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVAV 303

Query: 469 SVMLVVFVKTAVATIVAKAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYL 528
           SV+++V VKT V  ++A+ +G       Q   +L+Q GEFAFVL S AS+  L  G    
Sbjct: 304 SVIVLVAVKTLVLYLLARLYGIRSSERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMS 363

Query: 529 LLLGTTALSLVTTPLLFKLI 544
           LLL T  LS++TTPLL KL+
Sbjct: 364 LLLVTVTLSMMTTPLLMKLV 376

BLAST of CSPI03G16250 vs. ExPASy Swiss-Prot
Match: Q8BH01 (Transmembrane and coiled-coil domain-containing protein 3 OS=Mus musculus OX=10090 GN=Tmco3 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.6e-37
Identity = 146/502 (29.08%), Postives = 261/502 (51.99%), Query Frame = 0

Query: 63  MFDKVLEKEFSDND-LPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRA 122
           M D++LE      D L E     +F+ + + +    E V ++  E+     S K N T+ 
Sbjct: 179 MLDEILEDVRKAADRLEEEIEEHAFDDNKSVKGVNFEAVLRVEEEEA----SSKQNMTKR 238

Query: 123 FQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIG 182
                    E E+   ++ LID ++N ++++  +    + + D   I D+V + + +   
Sbjct: 239 ---------EVEDGLGLSMLIDSQNNQYILTKPRDS-TIPRADHHFIKDIVTIGMLSLPC 298

Query: 183 GIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKL 242
           G + + +G P + GY++ G ++GP GL  I  +VQVET+ +FGV F LF +GLEFS  KL
Sbjct: 299 GWLCTAIGLPTMFGYIICGVLLGPSGLNSIKSIVQVETLGEFGVFFTLFLVGLEFSPEKL 358

Query: 243 KVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLV---- 302
           + V  +++ G     ++ +        L   + ++ VF+ + LS+SST +V +FLV    
Sbjct: 359 RKVWRISLQGPCYMTLLMIAFGLWWGHLLRIRPTQSVFISTCLSLSSTPLVSRFLVGSAR 418

Query: 303 -ERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVL-----GGHNGLILGMIS----MGKL 362
            ++ + +  +  V +G L++QD  +GL  A++P L     G  + +++ ++     +G++
Sbjct: 419 GDKEAGDIDYSTVLLGMLVMQDVQLGLFIAVMPTLIQAGAGASSSVVMEVLRILFLIGQI 478

Query: 363 LLVL-SVYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSL 422
           L  L +V+L    + ++   P + KL ++ S    E+  L   AF  L    ++ L +S+
Sbjct: 479 LFSLAAVFLLCLVMKTYLIGPYYRKLHLE-SKGNKEILVLGVSAFTFLMLTVTELLDVSM 538

Query: 423 ELGSFVAGVMVSTTD--FGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLAS 482
           ELG F+AG +VS+      +  +  +EPIR+  A +F +SIG+ +   F+   L +L+  
Sbjct: 539 ELGCFLAGALVSSQGHMVTEEIMTYIEPIRDFLAIIFFASIGLHVFPTFVIYELTVLVFL 598

Query: 483 VMLVVFVKTAVATIVAKAFGYGIRTSFQ-----VGVMLAQIGEFAFVLLSRASNLHLIGG 542
            + VV +K  +A +V       +  S Q     V   LAQ+ EF+FVL SRA    ++  
Sbjct: 599 TLSVVIMKFVLAVLVLSLI---LPRSSQYIKWIVSAGLAQVSEFSFVLGSRARRAGILSR 658

BLAST of CSPI03G16250 vs. ExPASy TrEMBL
Match: A0A0A0LB52 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G177940 PE=4 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 9.5e-309
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60
           MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF
Sbjct: 1   MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR 120
           AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR
Sbjct: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR 120

Query: 121 AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180
           AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI
Sbjct: 121 AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180

Query: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK 240
           GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK
Sbjct: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK 240

Query: 241 LKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300
           LKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN
Sbjct: 241 LKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300

Query: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360
           SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI
Sbjct: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360

Query: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT 420
           LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT
Sbjct: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT 420

Query: 421 DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA 480
           DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA
Sbjct: 421 DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA 480

Query: 481 KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540
           KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF
Sbjct: 481 KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540

Query: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML
Sbjct: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 578

BLAST of CSPI03G16250 vs. ExPASy TrEMBL
Match: A0A1S3AXZ1 (K(+) efflux antiporter 5 OS=Cucumis melo OX=3656 GN=LOC103484011 PE=4 SV=1)

HSP 1 Score: 1055.0 bits (2727), Expect = 1.1e-304
Identity = 571/578 (98.79%), Postives = 574/578 (99.31%), Query Frame = 0

Query: 1   MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60
           MGFRFHMARR GAIGS LCI+VVLISS IHVTARSDKEIRERFYGNLINSTAPTSGDGSF
Sbjct: 1   MGFRFHMARRDGAIGSCLCIVVVLISSQIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR 120
           AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQK NGTR
Sbjct: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKTNGTR 120

Query: 121 AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180
           AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI
Sbjct: 121 AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180

Query: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK 240
           GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK
Sbjct: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK 240

Query: 241 LKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300
           LKVVGAVA+FGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN
Sbjct: 241 LKVVGAVAVFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300

Query: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360
           SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI
Sbjct: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360

Query: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT 420
           LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT
Sbjct: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT 420

Query: 421 DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA 480
           +FGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA
Sbjct: 421 EFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA 480

Query: 481 KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540
           KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF
Sbjct: 481 KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540

Query: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML
Sbjct: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 578

BLAST of CSPI03G16250 vs. ExPASy TrEMBL
Match: A0A5A7SSM4 (K(+) efflux antiporter 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G00090 PE=4 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 1.4e-299
Identity = 564/572 (98.60%), Postives = 567/572 (99.13%), Query Frame = 0

Query: 7   MARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK 66
           MARR  AIGS LCI+VVLISS IHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK
Sbjct: 1   MARRDCAIGSCLCIVVVLISSQIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK 60

Query: 67  VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQLQD 126
           VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQK NGTRAFQLQD
Sbjct: 61  VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKTNGTRAFQLQD 120

Query: 127 VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS 186
           VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS
Sbjct: 121 VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS 180

Query: 187 CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA 246
           CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA
Sbjct: 181 CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA 240

Query: 247 VAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH 306
           VA+FGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH
Sbjct: 241 VAVFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH 300

Query: 307 GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV 366
           GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV
Sbjct: 301 GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV 360

Query: 367 PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHT 426
           PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT+FGQHT
Sbjct: 361 PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTEFGQHT 420

Query: 427 LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG 486
           LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG
Sbjct: 421 LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG 480

Query: 487 IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAV 546
           IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAV
Sbjct: 481 IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAV 540

Query: 547 LNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           LNLGVLMHWFPSENNIQNEEKVSMIEAHNRML
Sbjct: 541 LNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 572

BLAST of CSPI03G16250 vs. ExPASy TrEMBL
Match: A0A5D3E372 (K(+) efflux antiporter 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1044G00070 PE=4 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 1.9e-296
Identity = 564/588 (95.92%), Postives = 567/588 (96.43%), Query Frame = 0

Query: 7   MARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK 66
           MARR  AIGS LCI+VVLISS IHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK
Sbjct: 1   MARRDCAIGSCLCIVVVLISSQIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK 60

Query: 67  VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQLQD 126
           VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQK NGTRAFQLQD
Sbjct: 61  VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKTNGTRAFQLQD 120

Query: 127 VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS 186
           VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS
Sbjct: 121 VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS 180

Query: 187 CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA 246
           CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA
Sbjct: 181 CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA 240

Query: 247 VAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH 306
           VA+FGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH
Sbjct: 241 VAVFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH 300

Query: 307 GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV 366
           GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV
Sbjct: 301 GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV 360

Query: 367 PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHT 426
           PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT+FGQHT
Sbjct: 361 PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTEFGQHT 420

Query: 427 LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG 486
           LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG
Sbjct: 421 LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG 480

Query: 487 IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSL------------- 546
           IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSL             
Sbjct: 481 IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVSYDCLCTVMHFP 540

Query: 547 ---VTTPLLFKLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
              VTTPLLFKLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML
Sbjct: 541 TLMVTTPLLFKLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 588

BLAST of CSPI03G16250 vs. ExPASy TrEMBL
Match: A0A6J1G2F9 (K(+) efflux antiporter 5 OS=Cucurbita moschata OX=3662 GN=LOC111450040 PE=4 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 2.1e-295
Identity = 551/575 (95.83%), Postives = 564/575 (98.09%), Query Frame = 0

Query: 4   RFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQM 63
           RF MARRGGAIGS+LCI++VLIS+ IHV+ARSDKEIRERFYGNLINSTAP SGDGS AQM
Sbjct: 4   RFFMARRGGAIGSWLCIVLVLISAQIHVSARSDKEIRERFYGNLINSTAPISGDGSIAQM 63

Query: 64  FDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQ 123
           FDKVLEKEF DNDLPEGSGGSSFN+SVADQEAELETVAKITHEKGKKNDSQKANGTRAFQ
Sbjct: 64  FDKVLEKEFPDNDLPEGSGGSSFNNSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQ 123

Query: 124 LQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGI 183
            QDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGI
Sbjct: 124 FQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGI 183

Query: 184 IFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKV 243
           IFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKV
Sbjct: 184 IFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKV 243

Query: 244 VGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSN 303
           VGAVA+FGGFLQIIIFMFLCGI+AMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSN
Sbjct: 244 VGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSN 303

Query: 304 TLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSW 363
           TLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLT ASILSW
Sbjct: 304 TLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTVASILSW 363

Query: 364 SFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDFG 423
           SFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVM+STTDFG
Sbjct: 364 SFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMISTTDFG 423

Query: 424 QHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAF 483
           QHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAV T+VAKAF
Sbjct: 424 QHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVVTVVAKAF 483

Query: 484 GYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLI 543
           GY IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPL+FKL+
Sbjct: 484 GYSIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLVFKLV 543

Query: 544 PAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           PAVLNLGVLMHWFPSEN IQ+EEK SMIEAHNRML
Sbjct: 544 PAVLNLGVLMHWFPSENIIQSEEKTSMIEAHNRML 578

BLAST of CSPI03G16250 vs. NCBI nr
Match: XP_004148124.1 (K(+) efflux antiporter 5 [Cucumis sativus] >KGN57301.1 hypothetical protein Csa_009498 [Cucumis sativus])

HSP 1 Score: 1068.5 bits (2762), Expect = 2.0e-308
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60
           MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF
Sbjct: 1   MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR 120
           AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR
Sbjct: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR 120

Query: 121 AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180
           AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI
Sbjct: 121 AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180

Query: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK 240
           GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK
Sbjct: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK 240

Query: 241 LKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300
           LKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN
Sbjct: 241 LKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300

Query: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360
           SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI
Sbjct: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360

Query: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT 420
           LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT
Sbjct: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT 420

Query: 421 DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA 480
           DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA
Sbjct: 421 DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA 480

Query: 481 KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540
           KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF
Sbjct: 481 KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540

Query: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML
Sbjct: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 578

BLAST of CSPI03G16250 vs. NCBI nr
Match: XP_008439121.1 (PREDICTED: K(+) efflux antiporter 5 [Cucumis melo])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.3e-304
Identity = 571/578 (98.79%), Postives = 574/578 (99.31%), Query Frame = 0

Query: 1   MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60
           MGFRFHMARR GAIGS LCI+VVLISS IHVTARSDKEIRERFYGNLINSTAPTSGDGSF
Sbjct: 1   MGFRFHMARRDGAIGSCLCIVVVLISSQIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR 120
           AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQK NGTR
Sbjct: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKTNGTR 120

Query: 121 AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180
           AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI
Sbjct: 121 AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180

Query: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK 240
           GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK
Sbjct: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK 240

Query: 241 LKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300
           LKVVGAVA+FGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN
Sbjct: 241 LKVVGAVAVFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300

Query: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360
           SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI
Sbjct: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360

Query: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT 420
           LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT
Sbjct: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT 420

Query: 421 DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA 480
           +FGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA
Sbjct: 421 EFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA 480

Query: 481 KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540
           KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF
Sbjct: 481 KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540

Query: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML
Sbjct: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 578

BLAST of CSPI03G16250 vs. NCBI nr
Match: XP_038905351.1 (K(+) efflux antiporter 5 [Benincasa hispida])

HSP 1 Score: 1039.6 bits (2687), Expect = 9.8e-300
Identity = 559/578 (96.71%), Postives = 570/578 (98.62%), Query Frame = 0

Query: 1   MGFRFHMARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSF 60
           MGFR+ M RRGGAIGS+LCI+VVLIS+ IHVTARSDKEIRERFYGNLINSTAPTSGDGS 
Sbjct: 1   MGFRYLMVRRGGAIGSWLCIVVVLISAQIHVTARSDKEIRERFYGNLINSTAPTSGDGSI 60

Query: 61  AQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR 120
           AQMFDKVLEKEFS+NDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR
Sbjct: 61  AQMFDKVLEKEFSENDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTR 120

Query: 121 AFQLQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180
           AFQ QDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI
Sbjct: 121 AFQFQDVFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAI 180

Query: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTK 240
           GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFI+EMVQVETVAQFGVVFLLFALGLEFSLTK
Sbjct: 181 GGIIFSCLGQPVIVGYLLAGSIIGPGGLKFINEMVQVETVAQFGVVFLLFALGLEFSLTK 240

Query: 241 LKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300
           LKVVGAVA+FGGFLQIIIFMFLCGI+AMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN
Sbjct: 241 LKVVGAVAVFGGFLQIIIFMFLCGIIAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERN 300

Query: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360
           SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI
Sbjct: 301 SSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASI 360

Query: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT 420
           LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVM+STT
Sbjct: 361 LSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMISTT 420

Query: 421 DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVA 480
           DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVA +VA
Sbjct: 421 DFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVAMVVA 480

Query: 481 KAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540
           KAFGY IRTSFQVG+MLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF
Sbjct: 481 KAFGYSIRTSFQVGIMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLF 540

Query: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           KLIPAVLNLGVLMHWFPSENNIQNEEK SMIEAHNRML
Sbjct: 541 KLIPAVLNLGVLMHWFPSENNIQNEEKASMIEAHNRML 578

BLAST of CSPI03G16250 vs. NCBI nr
Match: KAA0033578.1 (K(+) efflux antiporter 5 [Cucumis melo var. makuwa])

HSP 1 Score: 1038.1 bits (2683), Expect = 2.9e-299
Identity = 564/572 (98.60%), Postives = 567/572 (99.13%), Query Frame = 0

Query: 7   MARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK 66
           MARR  AIGS LCI+VVLISS IHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK
Sbjct: 1   MARRDCAIGSCLCIVVVLISSQIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK 60

Query: 67  VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQLQD 126
           VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQK NGTRAFQLQD
Sbjct: 61  VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKTNGTRAFQLQD 120

Query: 127 VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS 186
           VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS
Sbjct: 121 VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS 180

Query: 187 CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA 246
           CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA
Sbjct: 181 CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA 240

Query: 247 VAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH 306
           VA+FGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH
Sbjct: 241 VAVFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH 300

Query: 307 GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV 366
           GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV
Sbjct: 301 GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV 360

Query: 367 PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHT 426
           PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT+FGQHT
Sbjct: 361 PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTEFGQHT 420

Query: 427 LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG 486
           LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG
Sbjct: 421 LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG 480

Query: 487 IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAV 546
           IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAV
Sbjct: 481 IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAV 540

Query: 547 LNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           LNLGVLMHWFPSENNIQNEEKVSMIEAHNRML
Sbjct: 541 LNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 572

BLAST of CSPI03G16250 vs. NCBI nr
Match: TYK30334.1 (K(+) efflux antiporter 5 [Cucumis melo var. makuwa])

HSP 1 Score: 1027.7 bits (2656), Expect = 3.9e-296
Identity = 564/588 (95.92%), Postives = 567/588 (96.43%), Query Frame = 0

Query: 7   MARRGGAIGSFLCILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK 66
           MARR  AIGS LCI+VVLISS IHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK
Sbjct: 1   MARRDCAIGSCLCIVVVLISSQIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDK 60

Query: 67  VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQLQD 126
           VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQK NGTRAFQLQD
Sbjct: 61  VLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKTNGTRAFQLQD 120

Query: 127 VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS 186
           VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS
Sbjct: 121 VFSLENEESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFS 180

Query: 187 CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA 246
           CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA
Sbjct: 181 CLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGA 240

Query: 247 VAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH 306
           VA+FGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH
Sbjct: 241 VAVFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLH 300

Query: 307 GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV 366
           GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV
Sbjct: 301 GQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFV 360

Query: 367 PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHT 426
           PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTT+FGQHT
Sbjct: 361 PRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTEFGQHT 420

Query: 427 LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG 486
           LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG
Sbjct: 421 LDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYG 480

Query: 487 IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSL------------- 546
           IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSL             
Sbjct: 481 IRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVSYDCLCTVMHFP 540

Query: 547 ---VTTPLLFKLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
              VTTPLLFKLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML
Sbjct: 541 TLMVTTPLLFKLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 588

BLAST of CSPI03G16250 vs. TAIR 10
Match: AT5G51710.2 (K+ efflux antiporter 5 )

HSP 1 Score: 854.4 bits (2206), Expect = 5.4e-248
Identity = 449/556 (80.76%), Postives = 514/556 (92.45%), Query Frame = 0

Query: 21  LVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDKVLEKEFSDNDLPEG 80
           L  L+     ++ARSD+E RERFYGN++NSTAP +G+GS A+MFD+VLEKEFS+ND PEG
Sbjct: 9   LTFLLLLGTSLSARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFSENDSPEG 68

Query: 81  SGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQLQDVFSLENEESDDVTT 140
           S G+SFNSSVADQ+AE+ETVAK+THEKGK+ND+Q+ NGTR FQLQDVFSLENE+SDD+ T
Sbjct: 69  SDGASFNSSVADQQAEIETVAKVTHEKGKRNDTQENNGTRPFQLQDVFSLENEDSDDM-T 128

Query: 141 LIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCLGQPVIVGYLLAG 200
           LIDKK+NVFVMSNKKSKYP+LQVDLRLISDLVV+IV AAIGGI+FSCLGQPVIVGYLLAG
Sbjct: 129 LIDKKNNVFVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCLGQPVIVGYLLAG 188

Query: 201 SIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVAIFGGFLQIIIFM 260
           SIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFS+TKLKVVG VA+ GG LQI++ M
Sbjct: 189 SIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVAVLGGLLQIVLLM 248

Query: 261 FLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQVTIGTLILQDCA 320
           FLCG+ A+L GA+LSEG+FVG+FLSMSSTAVVVKFLVERNS+++LHGQVTIG LI QDC 
Sbjct: 249 FLCGVTALLCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQVTIGILIFQDCV 308

Query: 321 VGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFVPRFLKLMMQLSSQT 380
           VGLLFALLPVLGG++GL+ G+ISMGKLLL+LS+YLT AS+L+WSFVPRFLKLM+QLSSQT
Sbjct: 309 VGLLFALLPVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPRFLKLMIQLSSQT 368

Query: 381 NELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHTLDQVEPIRNLFAAL 440
           NELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVM+STT+F QHTL+QVEPIRNLFAAL
Sbjct: 369 NELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLEQVEPIRNLFAAL 428

Query: 441 FLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYGIRTSFQVGVMLAQI 500
           FLSSIGMLI+VHFLW+H+DILLASV+LV+ +KTA+A +V KAF Y +R SF VGV+LAQI
Sbjct: 429 FLSSIGMLINVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMRISFHVGVLLAQI 488

Query: 501 GEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAVLNLGVLMHWFPSEN 560
           GEFAFVLLSRASNLH+I GK+YLLLLGTTALSLVTTPLLFKLIP+ +NLGVL+ WFPSEN
Sbjct: 489 GEFAFVLLSRASNLHVIEGKMYLLLLGTTALSLVTTPLLFKLIPSAMNLGVLLRWFPSEN 548

Query: 561 NIQNEEKVSMIEAHNR 577
           +  NEEK S+IE HNR
Sbjct: 549 SSPNEEKASLIEVHNR 563

BLAST of CSPI03G16250 vs. TAIR 10
Match: AT5G51710.1 (K+ efflux antiporter 5 )

HSP 1 Score: 849.0 bits (2192), Expect = 2.3e-246
Identity = 449/559 (80.32%), Postives = 514/559 (91.95%), Query Frame = 0

Query: 21  LVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDKVLEKEFSDNDLPEG 80
           L  L+     ++ARSD+E RERFYGN++NSTAP +G+GS A+MFD+VLEKEFS+ND PEG
Sbjct: 9   LTFLLLLGTSLSARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFSENDSPEG 68

Query: 81  SGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQLQDVFSLENEESDDVTT 140
           S G+SFNSSVADQ+AE+ETVAK+THEKGK+ND+Q+ NGTR FQLQDVFSLENE+SDD+ T
Sbjct: 69  SDGASFNSSVADQQAEIETVAKVTHEKGKRNDTQENNGTRPFQLQDVFSLENEDSDDM-T 128

Query: 141 LIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCLGQPVIVGYLLAG 200
           LIDKK+NVFVMSNKKSKYP+LQVDLRLISDLVV+IV AAIGGI+FSCLGQPVIVGYLLAG
Sbjct: 129 LIDKKNNVFVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCLGQPVIVGYLLAG 188

Query: 201 SIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVAIFGGFLQIIIFM 260
           SIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFS+TKLKVVG VA+ GG LQI++ M
Sbjct: 189 SIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVAVLGGLLQIVLLM 248

Query: 261 FLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQVTIGTLILQDCA 320
           FLCG+ A+L GA+LSEG+FVG+FLSMSSTAVVVKFLVERNS+++LHGQVTIG LI QDC 
Sbjct: 249 FLCGVTALLCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQVTIGILIFQDCV 308

Query: 321 VGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFVPRFLKLMMQLSSQT 380
           VGLLFALLPVLGG++GL+ G+ISMGKLLL+LS+YLT AS+L+WSFVPRFLKLM+QLSSQT
Sbjct: 309 VGLLFALLPVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPRFLKLMIQLSSQT 368

Query: 381 NELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHTLDQVEPIRNLFAAL 440
           NELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVM+STT+F QHTL+QVEPIRNLFAAL
Sbjct: 369 NELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLEQVEPIRNLFAAL 428

Query: 441 FLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYGIRTSFQVGVMLAQI 500
           FLSSIGMLI+VHFLW+H+DILLASV+LV+ +KTA+A +V KAF Y +R SF VGV+LAQI
Sbjct: 429 FLSSIGMLINVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMRISFHVGVLLAQI 488

Query: 501 GEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAVLNLGVLMHWFPSEN 560
           GEFAFVLLSRASNLH+I GK+YLLLLGTTALSLVTTPLLFKLIP+ +NLGVL+ WFPSEN
Sbjct: 489 GEFAFVLLSRASNLHVIEGKMYLLLLGTTALSLVTTPLLFKLIPSAMNLGVLLRWFPSEN 548

Query: 561 NIQNE---EKVSMIEAHNR 577
           +  NE   EK S+IE HNR
Sbjct: 549 SSPNESLQEKASLIEVHNR 566

BLAST of CSPI03G16250 vs. TAIR 10
Match: AT5G11800.1 (K+ efflux antiporter 6 )

HSP 1 Score: 660.2 bits (1702), Expect = 1.5e-189
Identity = 372/586 (63.48%), Postives = 458/586 (78.16%), Query Frame = 0

Query: 9   RRGGAIGSFLCILVVLIS-----SHIHVTARSDKEIRERFYGNLINSTAPTSG------- 68
           RR       L +L++L+S     S     A SD ++ +    N  +S A  +        
Sbjct: 8   RRFSLSSQQLALLLLLLSFFLCFSVASPRAISDSDLLDETVANSSSSVASLNASSSSSLV 67

Query: 69  ---DGSFAQMFDKVLEKEFSDNDLPEGSGGSSFNSSVADQEAELETVAKITHEKGKKNDS 128
              +GSFA + D+ LEKEF+++D  E +   SFN+SVA Q+A LETVA++  +  KKN++
Sbjct: 68  KPKEGSFADIIDRALEKEFNESDQNEVADPGSFNNSVAGQQAVLETVARV--KSTKKNET 127

Query: 129 QKANGTRAFQLQDVFSLENE-ESDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLV 188
           ++    + FQL DVF+L N+  ++D  TLID+KDNVF++SN KSKYPVLQ+DLRLISDLV
Sbjct: 128 KE---EKRFQLHDVFNLNNDNRAEDTPTLIDRKDNVFIISNSKSKYPVLQLDLRLISDLV 187

Query: 189 VVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFAL 248
           VVIVSA  GGI F+C GQPVI GYLLAGSIIGPGGL FISEMVQVETVAQFGVVFLLFAL
Sbjct: 188 VVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGLNFISEMVQVETVAQFGVVFLLFAL 247

Query: 249 GLEFSLTKLKVVGAVAIFGGFLQIIIFMFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVV 308
           GLEFS  KLKVV +VA+ GG LQI++FMFLCGI   L G K SEGVFVG+FLSMSSTAVV
Sbjct: 248 GLEFSTAKLKVVRSVAVLGGLLQILLFMFLCGITVSLCGGKRSEGVFVGAFLSMSSTAVV 307

Query: 309 VKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLS 368
           +KFL+E+NS+N+LHGQVTIG LILQDCAVGLLFALLPVL G++G++ GM+S+GK++++L 
Sbjct: 308 LKFLMEKNSTNSLHGQVTIGILILQDCAVGLLFALLPVLEGNSGIVHGMLSIGKVVVLLL 367

Query: 369 VYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFV 428
            +L   SILS + +P  LKLM+ LSSQTNELYQLAAVAFCLL AWCSDKLGLSLELGSF 
Sbjct: 368 SFLAVLSILSRTCIPWLLKLMVSLSSQTNELYQLAAVAFCLLVAWCSDKLGLSLELGSFA 427

Query: 429 AGVMVSTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVK 488
           AGVM+STTD  +HTL+Q+EPIRNLFAALFL+SIGML++VHFLW+H+DILLASV+LV+ +K
Sbjct: 428 AGVMISTTDLAEHTLEQIEPIRNLFAALFLASIGMLVNVHFLWTHVDILLASVILVIIIK 487

Query: 489 TAVATIVAKAFGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALS 548
           T + T V K FGY  +T+  VG+ LAQIGEFAFVLLSRASNLHLI GK+YLLLLGTTALS
Sbjct: 488 TTIVTTVVKGFGYNNKTALLVGISLAQIGEFAFVLLSRASNLHLIEGKLYLLLLGTTALS 547

Query: 549 LVTTPLLFKLIPAVLNLGVLMHWFPSENNIQNEEKVSMIEAHNRML 579
           LVTTPL+FK+IPAV++LG+L+ WF  ++ I+  E V       RM+
Sbjct: 548 LVTTPLVFKMIPAVVHLGILLKWFSPDSTIEKGEIVRSESGKQRMI 588

BLAST of CSPI03G16250 vs. TAIR 10
Match: AT2G19600.1 (K+ efflux antiporter 4 )

HSP 1 Score: 644.8 bits (1662), Expect = 6.6e-185
Identity = 361/532 (67.86%), Postives = 427/532 (80.26%), Query Frame = 0

Query: 35  SDKEIRERFYGNLI--NSTAPTSGDGSFAQMFDKVLEKEFSDNDLPEGSGGSSFNSSVAD 94
           +D  +     G ++  N+T+    + SFA M D+ LEKEF DND  E     SFN+SVAD
Sbjct: 43  TDSVVTREINGTVVESNATSAKPREDSFADMIDRALEKEFPDNDQNEVPDPGSFNNSVAD 102

Query: 95  QEAELETVAKITHEKGKKNDSQKANGTRAFQLQDVFSLENEES-DDVTTLIDKKDNVFVM 154
           Q+A LETVA++   K KKN+      T+  + +  F+L+NE   +D   LID+KDNVF+M
Sbjct: 103 QQAVLETVARV---KPKKNE------TKTKEEKSFFNLDNENGVEDTPRLIDRKDNVFIM 162

Query: 155 SNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCLGQPVIVGYLLAGSIIGPGGLKFI 214
           SN KSKYPVLQ+DLRLISDLVVVIVSA  GGI F+C GQPVI GYLLAGSIIGPGGL F+
Sbjct: 163 SNPKSKYPVLQLDLRLISDLVVVIVSATCGGIAFACAGQPVITGYLLAGSIIGPGGLSFV 222

Query: 215 SEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVAIFGGFLQIIIFMFLCGIVAMLSG 274
           SEMVQVETVAQFGV+FLLFALGLEFS  KL+VV AVAI GG LQI +FM L GI A L G
Sbjct: 223 SEMVQVETVAQFGVIFLLFALGLEFSAAKLRVVRAVAIPGGLLQIFLFMCLSGITASLCG 282

Query: 275 AKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQVTIGTLILQDCAVGLLFALLPVL 334
            KL+EG+FVG+FLSMSSTAVV+KFL+ERNS + LHGQ+T+GTLILQDCAVGLLFALLPVL
Sbjct: 283 GKLTEGIFVGAFLSMSSTAVVLKFLMERNSISALHGQITVGTLILQDCAVGLLFALLPVL 342

Query: 335 GGHNGLILGMISMGKLLLVLSVYLTAASILSWSFVPRFLKLMMQLSSQTNELYQLAAVAF 394
           GG +G++ G++SM K L +L  +L A  +LS ++VP FLKLM  LSSQTNELYQLAAVAF
Sbjct: 343 GGTSGVLQGVLSMAKSLAILIAFLGALFVLSRTWVPWFLKLMTSLSSQTNELYQLAAVAF 402

Query: 395 CLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHTLDQVEPIRNLFAALFLSSIGMLIHV 454
           CLL AWCSDKLGLSLELGSF AGVM+STTD  QHTL+QVEPIRN FAALFL+SIGMLIH+
Sbjct: 403 CLLVAWCSDKLGLSLELGSFAAGVMISTTDLAQHTLEQVEPIRNFFAALFLASIGMLIHM 462

Query: 455 HFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYGIRTSFQVGVMLAQIGEFAFVLLSRA 514
           HFLW+H+DILLA+V+LV+ +KT V  IV K FGY  +T+  VG+ LAQIGEFAFVLLSRA
Sbjct: 463 HFLWNHVDILLAAVLLVIVIKTVVVAIVVKVFGYNNKTAVLVGMSLAQIGEFAFVLLSRA 522

Query: 515 SNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAVLNLGVLMHWFPSENNIQ 564
           SNLHLI  K+YLLLLGTTALSLVTTPLLFKLIPAV++LGVL+ WF  +++ +
Sbjct: 523 SNLHLIESKLYLLLLGTTALSLVTTPLLFKLIPAVVHLGVLLRWFSPDSSTE 565

BLAST of CSPI03G16250 vs. TAIR 10
Match: AT1G01790.1 (K+ efflux antiporter 1 )

HSP 1 Score: 144.8 bits (364), Expect = 2.1e-34
Identity = 111/357 (31.09%), Postives = 193/357 (54.06%), Query Frame = 0

Query: 189 GQPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVA 248
           G PV+ GYL AG +IGP GL  I  +     +A+FGVVFLLF +GLE S+ +L  +    
Sbjct: 612 GSPVL-GYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFLLFNIGLELSVERLSSMKKYV 671

Query: 249 IFGGFLQIIIFMFLCGIVA-MLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHG 308
              G  Q+++   + G++A  ++G      + +G+ L++SSTAVV++ L ER  S + HG
Sbjct: 672 FGLGSAQVLVTAAVVGLLAHYVAGQAGPAAIVIGNGLALSSTAVVLQVLQERGESTSRHG 731

Query: 309 QVTIGTLILQDCAVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFVP 368
           + +   L+ QD AV +L  L+P++  ++    G I    +   L +    A++   + + 
Sbjct: 732 RASFSVLLFQDLAVVVLLILIPLISPNSS--KGGIGFQAIAEALGLAAVKAAVAITAIIA 791

Query: 369 --RFL--KLMMQLSSQTN-ELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDF 428
             R L   +  Q++   N E++    +   L ++  + + GLS+ LG+F+AG++++ T+F
Sbjct: 792 GGRLLLRPIYKQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEF 851

Query: 429 GQHTLDQVEPIRNLFAALFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKA 488
                  + P R L   LF  ++GM I    L S+  +++ ++ L++  KT +  I+ K 
Sbjct: 852 SLQVESDIAPYRGLLLGLFFMTVGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKL 911

Query: 489 FGYGIRTSFQVGVMLAQIGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLL 540
           FG  I ++ +VG++LA  GEFAFV    A N  ++  ++  LL     +S+  TP L
Sbjct: 912 FGISIISAIRVGLLLAPGGEFAFVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWL 965