CSPI03G15810 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G15810
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptiongalactolipase DONGLE, chloroplastic-like
LocationChr3: 11834897 .. 11836633 (+)
RNA-Seq ExpressionCSPI03G15810
SyntenyCSPI03G15810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTACAACTTTGAAGCTTAATTCAATTCCCAATCATGTCACATTTTTTGAAACAACTCGTCCTATGTCGCATTCTTTTGGACAAGTATCCATACCCAGAAAATCCGATACTAATGTTTCTTCTTCTTCTTCTTCTTCTTCATCGTCGTCGGCTTCTCGACGGTTATCGGTTTCGTCTTCTTCAGTATTAGTACCGTTGATGGATGTATTTTCTTCTTCTTCTTTTTCTTCTTGTTCTCCGGCTGCTGCGGCTGCCATGGTTCAAAAAGTGGCCAGTTTGGCACGCTTGTGGAGGCAGATTCATGGCTGCAATGACTGGGAAGATCTCGTGGAGCCCACGCTTCACCCGCTTCTACGGCGAGAAATTATCCGCTACGGCGAGTTTGTAACGGCTTGTTATAAGGCTTTTGATCTTGATCCCAACTCTAAACGCTATTTGACCTGCAAATTTGGGAAGAAGAGTTTGTTGAAGGAAGTGGGTTTGGAGAGTTCAGGTTATGAAGTAACTAAATATATCTATGCTACTCCACCGGATATTAATATTCCTCCCATCCAAAACTCCCCGCCATCTTGCGGCCGATGGATCGGGTACGTTGCGGTTTCTTCCGACGAAACCAGCAAGAGACTTGGGCGACGAGATATAATCATTACTTTCAGAGGTACCGTCACCAACCCCGAATGGATTGCTAATCTAATGAGCTCCCTCACTCCTGCTCGCCTCGATCCTCACAATCATCGACCCGATGTCAAGGTTTGTTAATCTAACTCATGAACCCAAAAGCTTAAATATATCATCTCTAATTTTCTCTTTTAGCACAATTTAAATATCAACCCAAGAGCTAAAATAAGTTGAAGAAATTGATAAGTTCCAATAAATTAAATTACTTGAAATAGTTCTACACAGAACTTACTAAATAATTAACAATGCATAACCGCGAAGGAAAGAAAAGAAAAGTACCCTTTATTTTGATATCATGTTAAAAACAATTCAGCTTGGTGGCTTACGGTAAATTTAATATTTTTTCAGGTTGAATCAGGGTTTTTAACACTATACACGAGCGAAGAATCGAGCATGAAGTTCGGGCTCGAGAGTTGTCGGGAGCAGCTATTGTCAGAAGTGTCAAGGCTACTAAATAAGTACAAAGAGGAAGAAGTAAGCATAACCATGGCTGGGCATAGCATGGGGAGTGCATTAGCTCTTCTTCTAGCATATGACATAGCTGAATTAGGGTTGAATAGAAGAACAAACAACGAAGTGGTACCAGTTTCAGTATTCTCATTTGGAGGTCCAAGAGTTGGGAATTCAGGGTTCAAGAAAAGATGTGAAGAATTGGGAGTAAAAGTTTTAAGAATTGTGAATGTGAATGACCCCATCACAAAGATGCCAGGTGTTTTATTCAATGAGAATTTTAGGGTTTTTGGAGGTCTCTTAAATGGAGGAGCTAATAATAGCTATGAACATGTTGGAGTTGAATTGGTTCTTGATTTCTTCAACATGCAAAACCCTTCTTGTGTTCATGATTTGGAGACTTATATAAGCCTTTTAAGATGTCCAAAAAAACAAGAAGATGAAGATGAAGATGAAGATGAAGATAGTGGGAGAGGAAAGATTATTATTAACAGTGGGGAGTTTATCAACAAAGCTATGGAGTTTTTGTGTAGTAATGCACAAAGCTTGAACATGTTTCCATGGAGGAATCCAATGAATTACTTGAGTCAATCACAAAATTAG

mRNA sequence

ATGGCTGCTACAACTTTGAAGCTTAATTCAATTCCCAATCATGTCACATTTTTTGAAACAACTCGTCCTATGTCGCATTCTTTTGGACAAGTATCCATACCCAGAAAATCCGATACTAATGTTTCTTCTTCTTCTTCTTCTTCTTCATCGTCGTCGGCTTCTCGACGGTTATCGGTTTCGTCTTCTTCAGTATTAGTACCGTTGATGGATGTATTTTCTTCTTCTTCTTTTTCTTCTTGTTCTCCGGCTGCTGCGGCTGCCATGGTTCAAAAAGTGGCCAGTTTGGCACGCTTGTGGAGGCAGATTCATGGCTGCAATGACTGGGAAGATCTCGTGGAGCCCACGCTTCACCCGCTTCTACGGCGAGAAATTATCCGCTACGGCGAGTTTGTAACGGCTTGTTATAAGGCTTTTGATCTTGATCCCAACTCTAAACGCTATTTGACCTGCAAATTTGGGAAGAAGAGTTTGTTGAAGGAAGTGGGTTTGGAGAGTTCAGGTTATGAAGTAACTAAATATATCTATGCTACTCCACCGGATATTAATATTCCTCCCATCCAAAACTCCCCGCCATCTTGCGGCCGATGGATCGGGTACGTTGCGGTTTCTTCCGACGAAACCAGCAAGAGACTTGGGCGACGAGATATAATCATTACTTTCAGAGGTACCGTCACCAACCCCGAATGGATTGCTAATCTAATGAGCTCCCTCACTCCTGCTCGCCTCGATCCTCACAATCATCGACCCGATGTCAAGGTTGAATCAGGGTTTTTAACACTATACACGAGCGAAGAATCGAGCATGAAGTTCGGGCTCGAGAGTTGTCGGGAGCAGCTATTGTCAGAAGTGTCAAGGCTACTAAATAAGTACAAAGAGGAAGAAGTAAGCATAACCATGGCTGGGCATAGCATGGGGAGTGCATTAGCTCTTCTTCTAGCATATGACATAGCTGAATTAGGGTTGAATAGAAGAACAAACAACGAAGTGGTACCAGTTTCAGTATTCTCATTTGGAGGTCCAAGAGTTGGGAATTCAGGGTTCAAGAAAAGATGTGAAGAATTGGGAGTAAAAGTTTTAAGAATTGTGAATGTGAATGACCCCATCACAAAGATGCCAGGTGTTTTATTCAATGAGAATTTTAGGGTTTTTGGAGGTCTCTTAAATGGAGGAGCTAATAATAGCTATGAACATGTTGGAGTTGAATTGGTTCTTGATTTCTTCAACATGCAAAACCCTTCTTGTGTTCATGATTTGGAGACTTATATAAGCCTTTTAAGATGTCCAAAAAAACAAGAAGATGAAGATGAAGATGAAGATGAAGATAGTGGGAGAGGAAAGATTATTATTAACAGTGGGGAGTTTATCAACAAAGCTATGGAGTTTTTGTGTAGTAATGCACAAAGCTTGAACATGTTTCCATGGAGGAATCCAATGAATTACTTGAGTCAATCACAAAATTAG

Coding sequence (CDS)

ATGGCTGCTACAACTTTGAAGCTTAATTCAATTCCCAATCATGTCACATTTTTTGAAACAACTCGTCCTATGTCGCATTCTTTTGGACAAGTATCCATACCCAGAAAATCCGATACTAATGTTTCTTCTTCTTCTTCTTCTTCTTCATCGTCGTCGGCTTCTCGACGGTTATCGGTTTCGTCTTCTTCAGTATTAGTACCGTTGATGGATGTATTTTCTTCTTCTTCTTTTTCTTCTTGTTCTCCGGCTGCTGCGGCTGCCATGGTTCAAAAAGTGGCCAGTTTGGCACGCTTGTGGAGGCAGATTCATGGCTGCAATGACTGGGAAGATCTCGTGGAGCCCACGCTTCACCCGCTTCTACGGCGAGAAATTATCCGCTACGGCGAGTTTGTAACGGCTTGTTATAAGGCTTTTGATCTTGATCCCAACTCTAAACGCTATTTGACCTGCAAATTTGGGAAGAAGAGTTTGTTGAAGGAAGTGGGTTTGGAGAGTTCAGGTTATGAAGTAACTAAATATATCTATGCTACTCCACCGGATATTAATATTCCTCCCATCCAAAACTCCCCGCCATCTTGCGGCCGATGGATCGGGTACGTTGCGGTTTCTTCCGACGAAACCAGCAAGAGACTTGGGCGACGAGATATAATCATTACTTTCAGAGGTACCGTCACCAACCCCGAATGGATTGCTAATCTAATGAGCTCCCTCACTCCTGCTCGCCTCGATCCTCACAATCATCGACCCGATGTCAAGGTTGAATCAGGGTTTTTAACACTATACACGAGCGAAGAATCGAGCATGAAGTTCGGGCTCGAGAGTTGTCGGGAGCAGCTATTGTCAGAAGTGTCAAGGCTACTAAATAAGTACAAAGAGGAAGAAGTAAGCATAACCATGGCTGGGCATAGCATGGGGAGTGCATTAGCTCTTCTTCTAGCATATGACATAGCTGAATTAGGGTTGAATAGAAGAACAAACAACGAAGTGGTACCAGTTTCAGTATTCTCATTTGGAGGTCCAAGAGTTGGGAATTCAGGGTTCAAGAAAAGATGTGAAGAATTGGGAGTAAAAGTTTTAAGAATTGTGAATGTGAATGACCCCATCACAAAGATGCCAGGTGTTTTATTCAATGAGAATTTTAGGGTTTTTGGAGGTCTCTTAAATGGAGGAGCTAATAATAGCTATGAACATGTTGGAGTTGAATTGGTTCTTGATTTCTTCAACATGCAAAACCCTTCTTGTGTTCATGATTTGGAGACTTATATAAGCCTTTTAAGATGTCCAAAAAAACAAGAAGATGAAGATGAAGATGAAGATGAAGATAGTGGGAGAGGAAAGATTATTATTAACAGTGGGGAGTTTATCAACAAAGCTATGGAGTTTTTGTGTAGTAATGCACAAAGCTTGAACATGTTTCCATGGAGGAATCCAATGAATTACTTGAGTCAATCACAAAATTAG

Protein sequence

MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVSSSSVLVPLMDVFSSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNYLSQSQN*
Homology
BLAST of CSPI03G15810 vs. ExPASy Swiss-Prot
Match: Q9SIN9 (Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5 PE=1 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 3.9e-143
Identity = 270/438 (61.64%), Postives = 326/438 (74.43%), Query Frame = 0

Query: 58  SVSSSSVLVPLMDVFSSSSFSS------CSPAAAAAMVQKVASLARLWRQIHGCNDWEDL 117
           S SSSS+L P+  + +S   SS      C+P    +       L+R+WR+I GCN+W+DL
Sbjct: 47  SSSSSSLLAPV--ILNSPVASSSPPPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDL 106

Query: 118 VEPTLHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLES-SGYEV 177
           +EP L+PLL++EI RYG  V+ CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+V
Sbjct: 107 IEP-LNPLLQQEITRYGNLVSTCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQV 166

Query: 178 TKYIYATPP-DINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEW 237
           TKYIYATP  +INI PIQN      RW+GYVA SSD++ KRLGRRDI++TFRGTVTNPEW
Sbjct: 167 TKYIYATPDININISPIQNEMNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEW 226

Query: 238 IANLMSSLTPARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNK 297
           +AN MSSLTPAR  PHN R DVKVESGFL+LYTS+ES  KFGLESCR+QLLSE+SRL+NK
Sbjct: 227 LANFMSSLTPARFHPHNPRLDVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNK 286

Query: 298 YKEEEVSITMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKK 357
           YK EE+SIT+AGHSMGS+LA LLAYDIAELGLNRR     +PV+VFSF GPRVGN  FKK
Sbjct: 287 YKGEEMSITLAGHSMGSSLAQLLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKK 346

Query: 358 RCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGLLN-GGANNSYEHVGVELVLDFFN 417
           RCEELGVKVLRI NVNDP+TK+PGVLFNENFRV GG      + + Y HVGVEL LDFF+
Sbjct: 347 RCEELGVKVLRITNVNDPVTKLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFD 406

Query: 418 MQNPSCVHDLETYISLL---RCPKKQEDEDEDEDEDSGRGKIIINSGE---FINKAMEFL 477
           +QN SCVHDL+TYI LL   R   +  D DEDED D+   + +  +GE   F+ +     
Sbjct: 407 VQNISCVHDLQTYIDLLNQRRTNSRSVDSDEDEDSDNVALEFLKTNGEKMMFLKRQRMMY 466

Query: 478 CSNAQSLNMFPWRNPMNY 481
            SNA  L +F + N M+Y
Sbjct: 467 WSNAVDL-LFSFSNHMSY 480

BLAST of CSPI03G15810 vs. ExPASy Swiss-Prot
Match: Q9MA46 (Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.8e-140
Identity = 269/429 (62.70%), Postives = 327/429 (76.22%), Query Frame = 0

Query: 48  SSSSSASRRLSVSSSSVLVPL----MDVFSSSSFSSCSPAAAAAMVQKVASLARLWRQIH 107
           S S   S+RL VSSS++  P+    + + SSSS  +  P+ A A+      L+R+WR+I 
Sbjct: 32  SISQHTSKRLVVSSSTMSPPISSSPLSLPSSSSSQAIPPSRAPAV---TLPLSRVWREIQ 91

Query: 108 GCNDWEDLVEPTLHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGL 167
           G N+WE+L+EP L P+L++EI RYG  ++A YK FDL+PNSKRYL+CK+GKK+LLKE G+
Sbjct: 92  GSNNWENLIEP-LSPILQQEITRYGNLLSASYKGFDLNPNSKRYLSCKYGKKNLLKESGI 151

Query: 168 -ESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRG 227
            +  GY+VTKYIYAT PDIN+ PI+N  P+  RWIGYVAVSSDE+ KRLGRRDI++TFRG
Sbjct: 152 HDPDGYQVTKYIYAT-PDINLNPIKNE-PNRARWIGYVAVSSDESVKRLGRRDILVTFRG 211

Query: 228 TVTNPEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSE 287
           TVTN EW+ANL SSLTPARLDPHN RPDVKVESGFL LYTS ES  KFGLESCREQLLSE
Sbjct: 212 TVTNHEWLANLKSSLTPARLDPHNPRPDVKVESGFLGLYTSGESESKFGLESCREQLLSE 271

Query: 288 VSRLLNKYKEEEVSITMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRV 347
           +SRL+NK+K EE+SIT+AGHSMGS+LA LLAYDIAELG+N+R + + VPV+VFSF GPRV
Sbjct: 272 ISRLMNKHKGEEISITLAGHSMGSSLAQLLAYDIAELGMNQRRDEKPVPVTVFSFAGPRV 331

Query: 348 GNSGFKKRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGLLN-GGANNSYEHVGVE 407
           GN GFKKRCEELGVKVLRI NVNDPITK+PG LFNENFR  GG+     + + Y HVGVE
Sbjct: 332 GNLGFKKRCEELGVKVLRITNVNDPITKLPGFLFNENFRSLGGVYELPWSCSCYTHVGVE 391

Query: 408 LVLDFFNMQNPSCVHDLETYISLL---RCPKKQEDEDEDEDEDSGRGKIIINSGEFINKA 467
           L LDFF++QN SCVHDLETYI+L+   RC K   +ED                GEF+N+ 
Sbjct: 392 LTLDFFDVQNISCVHDLETYITLVNRPRCSKLAVNEDN-------------FGGEFLNRT 441

BLAST of CSPI03G15810 vs. ExPASy Swiss-Prot
Match: A2WY00 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=EG1 PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 3.7e-117
Identity = 220/400 (55.00%), Postives = 282/400 (70.50%), Query Frame = 0

Query: 81  SPAAAAAMVQKVA-SLARLWRQIHGCNDWEDLVEPTLHPLLRREIIRYGEFVTACYKAFD 140
           +P A     +K A ++A +WR++ GC DWE ++EP  HP+LR E+ RYGE V ACYKAFD
Sbjct: 41  APVARRGAARKTAETVAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFD 100

Query: 141 LDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGY 200
           LDP S+RYL CK+G++ +L+EVG+  +GYEVT+YIYA   D+++P ++ S    GRWIGY
Sbjct: 101 LDPASRRYLNCKYGRERMLEEVGMGGAGYEVTRYIYAA-ADVSVPTMEPSTSGRGRWIGY 160

Query: 201 VAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPARLDPHNHRPDVKVESGFLT 260
           VAVS+DE S+RLGRRD++++FRGTVT  EW+ANLMSSL  ARLDP + RPDVKVESGFL+
Sbjct: 161 VAVSTDEMSRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLS 220

Query: 261 LYTSEESSMKF-GLESCREQLLSEVSRLLNKYK--EEEVSITMAGHSMGSALALLLAYDI 320
           LYTS + + +F G  SCREQLL EVSRL+  Y    E+VS+T+AGHSMGSALALL AYD+
Sbjct: 221 LYTSADKTCRFGGAGSCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAYDL 280

Query: 321 AELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVNDPITKMPGVLF 380
           AELGLNR       PV+VFSFGGPRVGN+ FK RC+ELGVK LR+ NV+DPITK+PGV  
Sbjct: 281 AELGLNR-----AAPVTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFL 340

Query: 381 NENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRCPKKQEDEDE 440
           NE      G+L    ++ Y HVGVEL LDFF + + + VHDL TYISLLR   K++    
Sbjct: 341 NE---ATAGVLRPWRHSCYTHVGVELPLDFFKVGDLASVHDLATYISLLRGADKKQPAAA 400

Query: 441 DEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRN 477
             D           +G  + K M+F+    +     PW +
Sbjct: 401 AAD-----------AGGVLAKVMDFV-GRRRGGGALPWHD 419

BLAST of CSPI03G15810 vs. ExPASy Swiss-Prot
Match: Q8S1D9 (Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=EG1 PE=1 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 3.7e-117
Identity = 220/400 (55.00%), Postives = 282/400 (70.50%), Query Frame = 0

Query: 81  SPAAAAAMVQKVA-SLARLWRQIHGCNDWEDLVEPTLHPLLRREIIRYGEFVTACYKAFD 140
           +P A     +K A ++A +WR++ GC DWE ++EP  HP+LR E+ RYGE V ACYKAFD
Sbjct: 41  APVARRGAARKTAETVAGMWREVQGCGDWEGMLEPAPHPVLRGEVARYGELVGACYKAFD 100

Query: 141 LDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGY 200
           LDP S+RYL CK+G++ +L+EVG+  +GYEVT+YIYA   D+++P ++ S    GRWIGY
Sbjct: 101 LDPASRRYLNCKYGRERMLEEVGMGGAGYEVTRYIYAA-ADVSVPTMEPSTSGRGRWIGY 160

Query: 201 VAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPARLDPHNHRPDVKVESGFLT 260
           VAVS+DE S+RLGRRD++++FRGTVT  EW+ANLMSSL  ARLDP + RPDVKVESGFL+
Sbjct: 161 VAVSTDEMSRRLGRRDVLVSFRGTVTPAEWMANLMSSLEAARLDPCDPRPDVKVESGFLS 220

Query: 261 LYTSEESSMKF-GLESCREQLLSEVSRLLNKYK--EEEVSITMAGHSMGSALALLLAYDI 320
           LYTS + + +F G  SCREQLL EVSRL+  Y    E+VS+T+AGHSMGSALALL AYD+
Sbjct: 221 LYTSADKTCRFGGAGSCREQLLREVSRLVAAYSGGGEDVSVTLAGHSMGSALALLSAYDL 280

Query: 321 AELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVNDPITKMPGVLF 380
           AELGLNR       PV+VFSFGGPRVGN+ FK RC+ELGVK LR+ NV+DPITK+PGV  
Sbjct: 281 AELGLNR-----AAPVTVFSFGGPRVGNAAFKARCDELGVKALRVTNVHDPITKLPGVFL 340

Query: 381 NENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLETYISLLRCPKKQEDEDE 440
           NE      G+L    ++ Y HVGVEL LDFF + + + VHDL TYISLLR   K++    
Sbjct: 341 NE---ATAGVLRPWRHSCYTHVGVELPLDFFKVGDLASVHDLATYISLLRGADKKQPAAA 400

Query: 441 DEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRN 477
             D           +G  + K M+F+    +     PW +
Sbjct: 401 AAD-----------AGGVLAKVMDFV-GRRRGGGALPWHD 419

BLAST of CSPI03G15810 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 293.5 bits (750), Expect = 4.4e-78
Identity = 172/388 (44.33%), Postives = 233/388 (60.05%), Query Frame = 0

Query: 99  WRQIHGCNDWEDLVEPTLHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 158
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP S+   +C+F ++ L 
Sbjct: 89  WRKIQGEDDWAGLMDP-MDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLF 148

Query: 159 KEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVAVSSDE--TSKR 218
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D   T  R
Sbjct: 149 DSLGIIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCR 208

Query: 219 LGRRDIIITFRGTVTNPEWIANLMSSLTPARLDPHN-HRPDVKVESGFLTLYTSEESSMK 278
           LGRRDI I +RGTVT  EWIA+L   L P   +      P VK ESGFL LYT +++S  
Sbjct: 209 LGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCN 268

Query: 279 FGLESCREQLLSEVSRLLNKYKE---EEVSITMAGHSMGSALALLLAYDIAELGLNRRTN 338
           F   S REQ+L+EV RL+ +Y +   EE+SIT+ GHS+G ALA+L AYD+AE+G+NR   
Sbjct: 269 FSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRK 328

Query: 339 NEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGL 398
            +V+PV+ F++GGPRVGN  FK+R E+LGVKVLR+VN +D + K PG+  NE        
Sbjct: 329 GKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMK 388

Query: 399 LNGGANNSYEHVGVELVLDFFNMQNP--------SCVHDLETYISLLRCPKKQEDEDEDE 458
           L GG    Y HVG  L LD  + ++P        S  H+LE  + LL             
Sbjct: 389 LAGGLPWCYSHVGEMLPLD--HQKSPFLKPTVDLSTAHNLEALLHLL------------- 448

Query: 459 DEDSGRG-KIIINSGE---FINKAMEFL 463
           D   G+G + +++SG     +NKA +FL
Sbjct: 449 DGYHGKGQRFVLSSGRDPALVNKASDFL 459

BLAST of CSPI03G15810 vs. ExPASy TrEMBL
Match: A0A0A0L8A7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE=4 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 5.3e-276
Identity = 485/486 (99.79%), Postives = 485/486 (99.79%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60
           MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  SSSVLVPLMDVFSSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120
           SSSVLVPLMDVFSSSSFSSCS AAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240
           INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420
           IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY 480
           YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY
Sbjct: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY 480

Query: 481 LSQSQN 487
           LSQSQN
Sbjct: 481 LSQSQN 486

BLAST of CSPI03G15810 vs. ExPASy TrEMBL
Match: A0A5A7VKE2 (Galactolipase DONGLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00300 PE=4 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 6.7e-263
Identity = 469/492 (95.33%), Postives = 477/492 (96.95%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSS-SSSASRRLSV 60
           MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSD NVSSSSSSSS SSSASRRLSV
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  SSSSVLVPLMDVF--SSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120
           SSSSVLVPLMDVF  SSSSFSSCS AAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSL 240
           PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVT+PEWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYTSEES+MKFGLESCREQLLSEVSR+LNKYKEEEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFR FGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRCPKKQEDEDEDED---EDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPW 480
           LETYISLLRCPK+QED  +DED   + +GR KI INSGEFINKA EFLCSNAQSLNMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

Query: 481 RNPMNYLSQSQN 487
           RNPMNYLSQSQN
Sbjct: 481 RNPMNYLSQSQN 492

BLAST of CSPI03G15810 vs. ExPASy TrEMBL
Match: A0A1S3AXV1 (galactolipase DONGLE, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483973 PE=4 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 6.7e-263
Identity = 469/492 (95.33%), Postives = 477/492 (96.95%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSS-SSSASRRLSV 60
           MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSD NVSSSSSSSS SSSASRRLSV
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  SSSSVLVPLMDVF--SSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120
           SSSSVLVPLMDVF  SSSSFSSCS AAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSL 240
           PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVT+PEWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYTSEES+MKFGLESCREQLLSEVSR+LNKYKEEEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFR FGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRCPKKQEDEDEDED---EDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPW 480
           LETYISLLRCPK+QED  +DED   + +GR KI INSGEFINKA EFLCSNAQSLNMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

Query: 481 RNPMNYLSQSQN 487
           RNPMNYLSQSQN
Sbjct: 481 RNPMNYLSQSQN 492

BLAST of CSPI03G15810 vs. ExPASy TrEMBL
Match: A0A6J1GW19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458003 PE=4 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 1.8e-215
Identity = 403/489 (82.41%), Postives = 424/489 (86.71%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60
           MA TTLKLN   NHVTF ETTRP+SHSFGQVS+PRKSD +VSS+S  S SSS       S
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSD-HVSSTSRRSVSSS-------S 60

Query: 61  SSSVLVPLMDVF-SSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPL 120
           SSSVLVPL+D+F SSSS SS S +AA AMV KVA LARLWRQIHGCNDWE LVEPTLHPL
Sbjct: 61  SSSVLVPLIDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPL 120

Query: 121 LRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPP 180
           LRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPP
Sbjct: 121 LRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPP 180

Query: 181 DINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTP 240
           DINIPPIQNSPPSCGRWIGYVAVSSDETS+RLGRRD++ITFRGTVTN EWIANLMSSLTP
Sbjct: 181 DINIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTP 240

Query: 241 ARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITM 300
           ARLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSE+SRLLNKYK EEVSITM
Sbjct: 241 ARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITM 300

Query: 301 AGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVL 360
           AGHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FK+RCEELGVKVL
Sbjct: 301 AGHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVL 360

Query: 361 RIVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLE 420
           R+VNVNDPITKMPG        VFGG         YEHVGVELVLDFFNMQNPSCVHDLE
Sbjct: 361 RVVNVNDPITKMPG--------VFGG---------YEHVGVELVLDFFNMQNPSCVHDLE 420

Query: 421 TYISLLRCPKK--QEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNP 480
           TYISLLR PK+  QE +D+D    +GR K  IN G+FINKAMEFL     S NMFPWRNP
Sbjct: 421 TYISLLRYPKEEHQEHDDDDHHHHNGRRK-AINGGDFINKAMEFL-----SFNMFPWRNP 458

Query: 481 MNYLSQSQN 487
           MNYLSQSQN
Sbjct: 481 MNYLSQSQN 458

BLAST of CSPI03G15810 vs. ExPASy TrEMBL
Match: A0A6J1IP19 (phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479244 PE=4 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 1.2e-214
Identity = 397/486 (81.69%), Postives = 422/486 (86.83%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60
           MA TTLKLN   NHVTF ETTRPMSHSFGQVS+PRKSD +VSS+S  S SSS       S
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLPRKSD-HVSSTSRRSVSSS-------S 60

Query: 61  SSSVLVPLMDVFSSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120
           SSSVLVPL+D+FSSSS S+   +AA AMV K+A LARLWRQIHGCNDWE LVEPTLHPLL
Sbjct: 61  SSSVLVPLIDMFSSSSSSA---SAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLL 120

Query: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240
           INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRD++ITFRGTVTN EWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSE+SRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420
           +VNVNDPITK+PG        VFGG         YEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 VVNVNDPITKIPG--------VFGG---------YEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY 480
           YISLLR PK++  + +D+   +GR K +IN G+FINKAMEFL     S NM PWRNPMNY
Sbjct: 421 YISLLRYPKEEHQKHDDDHHHNGRRK-VINGGDFINKAMEFL-----SFNMLPWRNPMNY 452

Query: 481 LSQSQN 487
           LSQSQN
Sbjct: 481 LSQSQN 452

BLAST of CSPI03G15810 vs. NCBI nr
Match: XP_004148168.2 (galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical protein Csa_009777 [Cucumis sativus])

HSP 1 Score: 959.5 bits (2479), Expect = 1.1e-275
Identity = 485/486 (99.79%), Postives = 485/486 (99.79%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60
           MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60

Query: 61  SSSVLVPLMDVFSSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120
           SSSVLVPLMDVFSSSSFSSCS AAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL
Sbjct: 61  SSSVLVPLMDVFSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120

Query: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240
           INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420
           IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY 480
           YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY
Sbjct: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY 480

Query: 481 LSQSQN 487
           LSQSQN
Sbjct: 481 LSQSQN 486

BLAST of CSPI03G15810 vs. NCBI nr
Match: XP_008439078.1 (PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis melo] >KAA0067660.1 galactolipase DONGLE [Cucumis melo var. makuwa] >TYK23664.1 galactolipase DONGLE [Cucumis melo var. makuwa])

HSP 1 Score: 916.0 bits (2366), Expect = 1.4e-262
Identity = 469/492 (95.33%), Postives = 477/492 (96.95%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSS-SSSASRRLSV 60
           MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSD NVSSSSSSSS SSSASRRLSV
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDINVSSSSSSSSYSSSASRRLSV 60

Query: 61  SSSSVLVPLMDVF--SSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120
           SSSSVLVPLMDVF  SSSSFSSCS AAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH
Sbjct: 61  SSSSVLVPLMDVFSSSSSSFSSCSTAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLH 120

Query: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180
           PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT
Sbjct: 121 PLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYAT 180

Query: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSL 240
           PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVT+PEWIANLMSSL
Sbjct: 181 PPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTHPEWIANLMSSL 240

Query: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSI 300
           TPARLDPHNHRPDVKVESGFLTLYTSEES+MKFGLESCREQLLSEVSR+LNKYKEEEVSI
Sbjct: 241 TPARLDPHNHRPDVKVESGFLTLYTSEESNMKFGLESCREQLLSEVSRILNKYKEEEVSI 300

Query: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVK 360
           TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRC+ELGVK
Sbjct: 301 TMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCDELGVK 360

Query: 361 VLRIVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420
           VLRIVNVNDPITKMPGVLFNENFR FGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD
Sbjct: 361 VLRIVNVNDPITKMPGVLFNENFRAFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHD 420

Query: 421 LETYISLLRCPKKQEDEDEDED---EDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPW 480
           LETYISLLRCPK+QED  +DED   + +GR KI INSGEFINKA EFLCSNAQSLNMFPW
Sbjct: 421 LETYISLLRCPKQQEDRGQDEDNHGDHNGRRKITINSGEFINKAKEFLCSNAQSLNMFPW 480

Query: 481 RNPMNYLSQSQN 487
           RNPMNYLSQSQN
Sbjct: 481 RNPMNYLSQSQN 492

BLAST of CSPI03G15810 vs. NCBI nr
Match: XP_038886126.1 (galactolipase DONGLE, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 864.4 bits (2232), Expect = 4.8e-247
Identity = 442/489 (90.39%), Postives = 455/489 (93.05%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60
           MAATTLKLNSIPNHVTFFETTRPMSHSFGQV IP K +  +S SSS       SRRLSVS
Sbjct: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVLIPIKPNNVLSLSSS-------SRRLSVS 60

Query: 61  SSSVLVPLMDVFSSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120
           SS VLVPLMDVFS    SSCS AAAAAMV KVASLA LWRQIHGCNDWEDL+EPTLHPLL
Sbjct: 61  SSLVLVPLMDVFS----SSCSTAAAAAMVHKVASLAHLWRQIHGCNDWEDLMEPTLHPLL 120

Query: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKK+LL+EVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKNLLREVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240
           INI PIQNSPPSCGRWIGYVAVSSDETSKRLGRRDI+ITFRGTVTNPEWIANLMSSLT A
Sbjct: 181 INISPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIVITFRGTVTNPEWIANLMSSLTLA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYTSEE+SMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTSEETSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420
           IVNVNDPITKMPGVLFNENFRVFGGLLNGG NNSYEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGGNNSYEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRCPKKQEDEDEDEDED---SGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNP 480
           YISLLRC  KQE++ +D D+D   +GR KIIINSGEFINKAMEFLCSNAQ LNMFPWR P
Sbjct: 421 YISLLRCDPKQEEDHQDNDDDDHNNGRRKIIINSGEFINKAMEFLCSNAQILNMFPWRIP 478

Query: 481 MNYLSQSQN 487
           +NYLSQSQN
Sbjct: 481 VNYLSQSQN 478

BLAST of CSPI03G15810 vs. NCBI nr
Match: XP_022956251.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 758.4 bits (1957), Expect = 3.7e-215
Identity = 403/489 (82.41%), Postives = 424/489 (86.71%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60
           MA TTLKLN   NHVTF ETTRP+SHSFGQVS+PRKSD +VSS+S  S SSS       S
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPVSHSFGQVSLPRKSD-HVSSTSRRSVSSS-------S 60

Query: 61  SSSVLVPLMDVF-SSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPL 120
           SSSVLVPL+D+F SSSS SS S +AA AMV KVA LARLWRQIHGCNDWE LVEPTLHPL
Sbjct: 61  SSSVLVPLIDMFSSSSSSSSSSTSAAVAMVHKVAGLARLWRQIHGCNDWEHLVEPTLHPL 120

Query: 121 LRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPP 180
           LRRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPP
Sbjct: 121 LRREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPP 180

Query: 181 DINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTP 240
           DINIPPIQNSPPSCGRWIGYVAVSSDETS+RLGRRD++ITFRGTVTN EWIANLMSSLTP
Sbjct: 181 DINIPPIQNSPPSCGRWIGYVAVSSDETSERLGRRDVVITFRGTVTNSEWIANLMSSLTP 240

Query: 241 ARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITM 300
           ARLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSE+SRLLNKYK EEVSITM
Sbjct: 241 ARLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITM 300

Query: 301 AGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVL 360
           AGHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FK+RCEELGVKVL
Sbjct: 301 AGHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVL 360

Query: 361 RIVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLE 420
           R+VNVNDPITKMPG        VFGG         YEHVGVELVLDFFNMQNPSCVHDLE
Sbjct: 361 RVVNVNDPITKMPG--------VFGG---------YEHVGVELVLDFFNMQNPSCVHDLE 420

Query: 421 TYISLLRCPKK--QEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNP 480
           TYISLLR PK+  QE +D+D    +GR K  IN G+FINKAMEFL     S NMFPWRNP
Sbjct: 421 TYISLLRYPKEEHQEHDDDDHHHHNGRRK-AINGGDFINKAMEFL-----SFNMFPWRNP 458

Query: 481 MNYLSQSQN 487
           MNYLSQSQN
Sbjct: 481 MNYLSQSQN 458

BLAST of CSPI03G15810 vs. NCBI nr
Match: XP_022979567.1 (phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 755.7 bits (1950), Expect = 2.4e-214
Identity = 397/486 (81.69%), Postives = 422/486 (86.83%), Query Frame = 0

Query: 1   MAATTLKLNSIPNHVTFFETTRPMSHSFGQVSIPRKSDTNVSSSSSSSSSSSASRRLSVS 60
           MA TTLKLN   NHVTF ETTRPMSHSFGQVS+PRKSD +VSS+S  S SSS       S
Sbjct: 1   MATTTLKLNLNINHVTFSETTRPMSHSFGQVSLPRKSD-HVSSTSRRSVSSS-------S 60

Query: 61  SSSVLVPLMDVFSSSSFSSCSPAAAAAMVQKVASLARLWRQIHGCNDWEDLVEPTLHPLL 120
           SSSVLVPL+D+FSSSS S+   +AA AMV K+A LARLWRQIHGCNDWE LVEPTLHPLL
Sbjct: 61  SSSVLVPLIDMFSSSSSSA---SAAVAMVHKMAGLARLWRQIHGCNDWEHLVEPTLHPLL 120

Query: 121 RREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180
           RRE+IRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD
Sbjct: 121 RREVIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLESSGYEVTKYIYATPPD 180

Query: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEWIANLMSSLTPA 240
           INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRD++ITFRGTVTN EWIANLMSSLTPA
Sbjct: 181 INIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDVVITFRGTVTNSEWIANLMSSLTPA 240

Query: 241 RLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNKYKEEEVSITMA 300
           RLDPHNHRPDVKVESGFLTLYT EE+S KFGLESCREQLLSE+SRLLNKYK EEVSITMA
Sbjct: 241 RLDPHNHRPDVKVESGFLTLYTCEETSTKFGLESCREQLLSEISRLLNKYKSEEVSITMA 300

Query: 301 GHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLR 360
           GHSMGSALALLLAYDIAELGLN+RTN EVVPVSVFSFGGPRVGNS FK+RCEELGVKVLR
Sbjct: 301 GHSMGSALALLLAYDIAELGLNKRTNKEVVPVSVFSFGGPRVGNSRFKQRCEELGVKVLR 360

Query: 361 IVNVNDPITKMPGVLFNENFRVFGGLLNGGANNSYEHVGVELVLDFFNMQNPSCVHDLET 420
           +VNVNDPITK+PG        VFGG         YEHVGVELVLDFFNMQNPSCVHDLET
Sbjct: 361 VVNVNDPITKIPG--------VFGG---------YEHVGVELVLDFFNMQNPSCVHDLET 420

Query: 421 YISLLRCPKKQEDEDEDEDEDSGRGKIIINSGEFINKAMEFLCSNAQSLNMFPWRNPMNY 480
           YISLLR PK++  + +D+   +GR K +IN G+FINKAMEFL     S NM PWRNPMNY
Sbjct: 421 YISLLRYPKEEHQKHDDDHHHNGRRK-VINGGDFINKAMEFL-----SFNMLPWRNPMNY 452

Query: 481 LSQSQN 487
           LSQSQN
Sbjct: 481 LSQSQN 452

BLAST of CSPI03G15810 vs. TAIR 10
Match: AT2G31690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 509.6 bits (1311), Expect = 2.8e-144
Identity = 270/438 (61.64%), Postives = 326/438 (74.43%), Query Frame = 0

Query: 58  SVSSSSVLVPLMDVFSSSSFSS------CSPAAAAAMVQKVASLARLWRQIHGCNDWEDL 117
           S SSSS+L P+  + +S   SS      C+P    +       L+R+WR+I GCN+W+DL
Sbjct: 47  SSSSSSLLAPV--ILNSPVASSSPPPIYCAPKFPCSSGAATVPLSRVWREIQGCNNWKDL 106

Query: 118 VEPTLHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGLES-SGYEV 177
           +EP L+PLL++EI RYG  V+ CYKAFDLDPNSKRYL CK+GK++LLKE  ++    Y+V
Sbjct: 107 IEP-LNPLLQQEITRYGNLVSTCYKAFDLDPNSKRYLNCKYGKQTLLKETEIDQPEDYQV 166

Query: 178 TKYIYATPP-DINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRGTVTNPEW 237
           TKYIYATP  +INI PIQN      RW+GYVA SSD++ KRLGRRDI++TFRGTVTNPEW
Sbjct: 167 TKYIYATPDININISPIQNEMNRRARWVGYVAASSDDSVKRLGRRDIVVTFRGTVTNPEW 226

Query: 238 IANLMSSLTPARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSEVSRLLNK 297
           +AN MSSLTPAR  PHN R DVKVESGFL+LYTS+ES  KFGLESCR+QLLSE+SRL+NK
Sbjct: 227 LANFMSSLTPARFHPHNPRLDVKVESGFLSLYTSDESESKFGLESCRQQLLSEISRLMNK 286

Query: 298 YKEEEVSITMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRVGNSGFKK 357
           YK EE+SIT+AGHSMGS+LA LLAYDIAELGLNRR     +PV+VFSF GPRVGN  FKK
Sbjct: 287 YKGEEMSITLAGHSMGSSLAQLLAYDIAELGLNRRIGKGDIPVTVFSFAGPRVGNLEFKK 346

Query: 358 RCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGLLN-GGANNSYEHVGVELVLDFFN 417
           RCEELGVKVLRI NVNDP+TK+PGVLFNENFRV GG      + + Y HVGVEL LDFF+
Sbjct: 347 RCEELGVKVLRITNVNDPVTKLPGVLFNENFRVLGGFYELPWSCSCYVHVGVELTLDFFD 406

Query: 418 MQNPSCVHDLETYISLL---RCPKKQEDEDEDEDEDSGRGKIIINSGE---FINKAMEFL 477
           +QN SCVHDL+TYI LL   R   +  D DEDED D+   + +  +GE   F+ +     
Sbjct: 407 VQNISCVHDLQTYIDLLNQRRTNSRSVDSDEDEDSDNVALEFLKTNGEKMMFLKRQRMMY 466

Query: 478 CSNAQSLNMFPWRNPMNY 481
            SNA  L +F + N M+Y
Sbjct: 467 WSNAVDL-LFSFSNHMSY 480

BLAST of CSPI03G15810 vs. TAIR 10
Match: AT1G05800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.3e-141
Identity = 269/429 (62.70%), Postives = 327/429 (76.22%), Query Frame = 0

Query: 48  SSSSSASRRLSVSSSSVLVPL----MDVFSSSSFSSCSPAAAAAMVQKVASLARLWRQIH 107
           S S   S+RL VSSS++  P+    + + SSSS  +  P+ A A+      L+R+WR+I 
Sbjct: 32  SISQHTSKRLVVSSSTMSPPISSSPLSLPSSSSSQAIPPSRAPAV---TLPLSRVWREIQ 91

Query: 108 GCNDWEDLVEPTLHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEVGL 167
           G N+WE+L+EP L P+L++EI RYG  ++A YK FDL+PNSKRYL+CK+GKK+LLKE G+
Sbjct: 92  GSNNWENLIEP-LSPILQQEITRYGNLLSASYKGFDLNPNSKRYLSCKYGKKNLLKESGI 151

Query: 168 -ESSGYEVTKYIYATPPDINIPPIQNSPPSCGRWIGYVAVSSDETSKRLGRRDIIITFRG 227
            +  GY+VTKYIYAT PDIN+ PI+N  P+  RWIGYVAVSSDE+ KRLGRRDI++TFRG
Sbjct: 152 HDPDGYQVTKYIYAT-PDINLNPIKNE-PNRARWIGYVAVSSDESVKRLGRRDILVTFRG 211

Query: 228 TVTNPEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLESCREQLLSE 287
           TVTN EW+ANL SSLTPARLDPHN RPDVKVESGFL LYTS ES  KFGLESCREQLLSE
Sbjct: 212 TVTNHEWLANLKSSLTPARLDPHNPRPDVKVESGFLGLYTSGESESKFGLESCREQLLSE 271

Query: 288 VSRLLNKYKEEEVSITMAGHSMGSALALLLAYDIAELGLNRRTNNEVVPVSVFSFGGPRV 347
           +SRL+NK+K EE+SIT+AGHSMGS+LA LLAYDIAELG+N+R + + VPV+VFSF GPRV
Sbjct: 272 ISRLMNKHKGEEISITLAGHSMGSSLAQLLAYDIAELGMNQRRDEKPVPVTVFSFAGPRV 331

Query: 348 GNSGFKKRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGLLN-GGANNSYEHVGVE 407
           GN GFKKRCEELGVKVLRI NVNDPITK+PG LFNENFR  GG+     + + Y HVGVE
Sbjct: 332 GNLGFKKRCEELGVKVLRITNVNDPITKLPGFLFNENFRSLGGVYELPWSCSCYTHVGVE 391

Query: 408 LVLDFFNMQNPSCVHDLETYISLL---RCPKKQEDEDEDEDEDSGRGKIIINSGEFINKA 467
           L LDFF++QN SCVHDLETYI+L+   RC K   +ED                GEF+N+ 
Sbjct: 392 LTLDFFDVQNISCVHDLETYITLVNRPRCSKLAVNEDN-------------FGGEFLNRT 441

BLAST of CSPI03G15810 vs. TAIR 10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 293.5 bits (750), Expect = 3.1e-79
Identity = 172/388 (44.33%), Postives = 233/388 (60.05%), Query Frame = 0

Query: 99  WRQIHGCNDWEDLVEPTLHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 158
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP S+   +C+F ++ L 
Sbjct: 89  WRKIQGEDDWAGLMDP-MDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLF 148

Query: 159 KEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVAVSSDE--TSKR 218
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D   T  R
Sbjct: 149 DSLGIIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCR 208

Query: 219 LGRRDIIITFRGTVTNPEWIANLMSSLTPARLDPHN-HRPDVKVESGFLTLYTSEESSMK 278
           LGRRDI I +RGTVT  EWIA+L   L P   +      P VK ESGFL LYT +++S  
Sbjct: 209 LGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCN 268

Query: 279 FGLESCREQLLSEVSRLLNKYKE---EEVSITMAGHSMGSALALLLAYDIAELGLNRRTN 338
           F   S REQ+L+EV RL+ +Y +   EE+SIT+ GHS+G ALA+L AYD+AE+G+NR   
Sbjct: 269 FSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRK 328

Query: 339 NEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGL 398
            +V+PV+ F++GGPRVGN  FK+R E+LGVKVLR+VN +D + K PG+  NE        
Sbjct: 329 GKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMK 388

Query: 399 LNGGANNSYEHVGVELVLDFFNMQNP--------SCVHDLETYISLLRCPKKQEDEDEDE 458
           L GG    Y HVG  L LD  + ++P        S  H+LE  + LL             
Sbjct: 389 LAGGLPWCYSHVGEMLPLD--HQKSPFLKPTVDLSTAHNLEALLHLL------------- 448

Query: 459 DEDSGRG-KIIINSGE---FINKAMEFL 463
           D   G+G + +++SG     +NKA +FL
Sbjct: 449 DGYHGKGQRFVLSSGRDPALVNKASDFL 459

BLAST of CSPI03G15810 vs. TAIR 10
Match: AT1G06800.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 289.7 bits (740), Expect = 4.5e-78
Identity = 162/347 (46.69%), Postives = 216/347 (62.25%), Query Frame = 0

Query: 99  WRQIHGCNDWEDLVEPTLHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLL 158
           WR+I G +DW  L++P + P+LR E+IRYGE   ACY AFD DP S+   +C+F ++ L 
Sbjct: 89  WRKIQGEDDWAGLMDP-MDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLF 148

Query: 159 KEVGLESSGYEVTKYIYATPPDINIPPIQNSP------PSCGRWIGYVAVSSDE--TSKR 218
             +G+  SGYEV +Y+YAT  +IN+P   +             W+GYVAVS D   T  R
Sbjct: 149 DSLGIIDSGYEVARYLYAT-SNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCR 208

Query: 219 LGRRDIIITFRGTVTNPEWIANLMSSLTPARLDPHN-HRPDVKVESGFLTLYTSEESSMK 278
           LGRRDI I +RGTVT  EWIA+L   L P   +      P VK ESGFL LYT +++S  
Sbjct: 209 LGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCN 268

Query: 279 FGLESCREQLLSEVSRLLNKYKE---EEVSITMAGHSMGSALALLLAYDIAELGLNRRTN 338
           F   S REQ+L+EV RL+ +Y +   EE+SIT+ GHS+G ALA+L AYD+AE+G+NR   
Sbjct: 269 FSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRK 328

Query: 339 NEVVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGL 398
            +V+PV+ F++GGPRVGN  FK+R E+LGVKVLR+VN +D + K PG+  NE        
Sbjct: 329 GKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMK 388

Query: 399 LNGGANNSYEHVGVELVLDFFNMQNP--------SCVHDLETYISLL 426
           L GG    Y HVG  L LD  + ++P        S  H+LE  + LL
Sbjct: 389 LAGGLPWCYSHVGEMLPLD--HQKSPFLKPTVDLSTAHNLEALLHLL 431

BLAST of CSPI03G15810 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 280.4 bits (716), Expect = 2.7e-75
Identity = 184/444 (41.44%), Postives = 255/444 (57.43%), Query Frame = 0

Query: 42  SSSSSSSSSSSASRRLSVSSSSVLVPLMDVFSSSSFSSCSPAAAAAMVQKVASLARLWRQ 101
           SSS +S SSS+  ++ S   + V           S +     A     +K  SL  +WR+
Sbjct: 45  SSSCTSISSSTTQQKQSNKQTHV-----------SDNKREEKAEEEEEEKEVSLREIWRE 104

Query: 102 IHGCNDWEDLVEPTLHPLLRREIIRYGEFVTACYKAFDLDPNSKRYLTCKFGKKSLLKEV 161
           + GCN+WE  ++P ++  LRREIIRYGEF  ACY +FD DP+SK   +CK+        +
Sbjct: 105 VQGCNNWEGQLDP-MNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFLNL 164

Query: 162 GLE-SSGYEVTKYIYATPPDINIPP-IQNSPPSC-----GRWIGYVAVSSDETS-KRLGR 221
            L    GY +T+Y+YAT  +IN+P   Q S  S        W+G+VAV++DE    RLGR
Sbjct: 165 DLHLHKGYTITRYLYAT-SNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEVSRLGR 224

Query: 222 RDIIITFRGTVTNPEWIANLMSSLTPARLDPHNHRPDVKVESGFLTLYTSEESSMKFGLE 281
           RDI+I +RGTVT  EWI +L   L  A        P +K+E GF  LYT +E S KF   
Sbjct: 225 RDIVIAWRGTVTYLEWIYDLKDILCSANF---GDDPSIKIELGFHDLYTKKEDSCKFSSF 284

Query: 282 SCREQLLSEVSRLLNKYKEEE----VSITMAGHSMGSALALLLAYDIAELGLNR-RTNNE 341
           S REQ+L+EV RL+  Y  EE     SIT+ GHS+G++LAL+ AYDIAEL LN    NN 
Sbjct: 285 SAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPENNY 344

Query: 342 VVPVSVFSFGGPRVGNSGFKKRCEELGVKVLRIVNVNDPITKMPGVLFNENFRVFGGLLN 401
            +P++VFSF GPRVGN  FK+RC+ELGVKVLR+VNV+D +  +PG+  NE F+ F   + 
Sbjct: 345 KIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQ-FQKYVE 404

Query: 402 GGANN--SYEHVGVELVLDFFN------MQNPSCVHDLETYISLLRCPKKQEDEDEDEDE 461
              +   SY HVGVEL LD          ++  C H+LE  + L+       D    +DE
Sbjct: 405 EKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLV-------DGYHGKDE 464

Query: 462 DSGRGKIIINSGE--FINKAMEFL 463
           ++ +   ++   +   +NK+ +FL
Sbjct: 465 EAEKRFCLVTKRDIALVNKSCDFL 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIN93.9e-14361.64Phospholipase A1-Ialpha2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL5... [more]
Q9MA461.8e-14062.70Galactolipase DONGLE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGL PE=1 ... [more]
A2WY003.7e-11755.00Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica ... [more]
Q8S1D93.7e-11755.00Phospholipase A1 EG1, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonic... [more]
Q941F14.4e-7844.33Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4... [more]
Match NameE-valueIdentityDescription
A0A0A0L8A75.3e-27699.79Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G174570 PE... [more]
A0A5A7VKE26.7e-26395.33Galactolipase DONGLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577... [more]
A0A1S3AXV16.7e-26395.33galactolipase DONGLE, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483973... [more]
A0A6J1GW191.8e-21582.41phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IP191.2e-21481.69phospholipase A1-Ialpha2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_004148168.21.1e-27599.79galactolipase DONGLE, chloroplastic [Cucumis sativus] >KGN57254.1 hypothetical p... [more]
XP_008439078.11.4e-26295.33PREDICTED: galactolipase DONGLE, chloroplastic-like [Cucumis melo] >KAA0067660.1... [more]
XP_038886126.14.8e-24790.39galactolipase DONGLE, chloroplastic-like [Benincasa hispida][more]
XP_022956251.13.7e-21582.41phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita moschata][more]
XP_022979567.12.4e-21481.69phospholipase A1-Ialpha2, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G31690.12.8e-14461.64alpha/beta-Hydrolases superfamily protein [more]
AT1G05800.11.3e-14162.70alpha/beta-Hydrolases superfamily protein [more]
AT1G06800.13.1e-7944.33alpha/beta-Hydrolases superfamily protein [more]
AT1G06800.24.5e-7846.69alpha/beta-Hydrolases superfamily protein [more]
AT1G51440.12.7e-7541.44alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 276..296
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..53
NoneNo IPR availablePANTHERPTHR31403:SF4PHOSPHOLIPASE A1-IALPHA2, CHLOROPLASTICcoord: 21..476
NoneNo IPR availablePANTHERPTHR31403PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTICcoord: 21..476
NoneNo IPR availableCDDcd00519Lipase_3coord: 161..426
e-value: 1.23471E-49
score: 167.653
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 98..471
e-value: 1.1E-99
score: 336.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 177..424
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 217..374
e-value: 7.4E-38
score: 129.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G15810.1CSPI03G15810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009695 jasmonic acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0008970 phospholipase A1 activity