CSPI03G15690 (gene) Cucumber (PI 183967) v1

BLAST of CSPI03G15690 vs. ExPASy Swiss-Prot
Match: F4ISQ7 (DNA topoisomerase 3-beta OS=Arabidopsis thaliana OX=3702 GN=At2g32000 PE=2 SV=1)

HSP 1 Score: 1403.7 bits (3632), Expect = 0.0e+00
Identity = 664/862 (77.03%), Postives = 751/862 (87.12%), Query Frame = 0

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GSTEVHEFDG F G  A+Y+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  QAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENICF 125
              +Q+W + DP DLF A +IK ESNPKAHIC+HLS EARGC  +VLWLDCDREGENICF
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 EVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKVGV 185
           EVIE TGF    +++KVYRARFSSVTEKDI KA+DNLVEPNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 AFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYILQT 245
           AF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI + 
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKVAS 305
           GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRMLL 365
           SALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL   V NP WG YV+ LL
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTPPD 485
           + SI GE F+CTG RVT KGFT+IMPW AV+EKKLP FLKGE+IEV RV+LY+GNT PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQFS 605
           PDLCLPDIRSFIE QITLVAKGQADHS VVQ V+QQFR+K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFE 665
           PLADSGRALSKCGKCLRYMK I++ P RL+CG CEEVYYLPQKGT+KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPHPT 725
           L+IYS+PGPEGKSFPLCPYCYNSPPFEGI+TL+GASK   +   +  G GMPC +CPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCADC 785
           C+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TT ++C +C
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF+R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGGRGRSSGKPMDPKMSFRDF 868
           GRGGR  S  K +DPKMSFRDF
Sbjct: 847 GRGGRRGS--KSVDPKMSFRDF 865

BLAST of CSPI03G15690 vs. ExPASy Swiss-Prot
Match: Q0J0S6 (DNA topoisomerase 3-beta OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3B PE=2 SV=1)

HSP 1 Score: 1338.2 bits (3462), Expect = 0.0e+00
Identity = 635/868 (73.16%), Postives = 735/868 (84.68%), Query Frame = 0

Query: 2   VAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVF 61
           +APTVLMVAEKPSIALSIASALS G+MSTRKGST+VHEFDG F G HA++KVTSVIGHV 
Sbjct: 1   MAPTVLMVAEKPSIALSIASALSGGRMSTRKGSTDVHEFDGMFQGSHAFFKVTSVIGHVL 60

Query: 62  SIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGE 121
           S+DF   +Q+W+ TDP+DLF A V+++E NPKAHI +HL++EARGC  LVLWLDCDREGE
Sbjct: 61  SVDFPPAYQNWEGTDPMDLFVAPVLRSECNPKAHIRRHLAQEARGCTYLVLWLDCDREGE 120

Query: 122 NICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDL 181
           NIC+EVI+CTG   +   ++++RA+FSSVTEKDI+ A++NLV P+++EALAVDARQEIDL
Sbjct: 121 NICYEVIDCTGIPKSEVGRRIFRAKFSSVTEKDIMDAMNNLVLPSKDEALAVDARQEIDL 180

Query: 182 KVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPY 241
           KVGVAFTRFQT +FQGKYGNLDSR+ISYGPCQTPTLGFCVQR+ QITTFKPEKFWS+  Y
Sbjct: 181 KVGVAFTRFQTRYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYQQITTFKPEKFWSLKTY 240

Query: 242 ILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLL 301
           +++ G E+ L+W+R KLFD DVT MFQK V  DGI +VT+IS K+E K RP GLNTVNLL
Sbjct: 241 VIKDGNEIQLEWDRKKLFDFDVTVMFQKMVASDGILKVTDISVKEECKARPPGLNTVNLL 300

Query: 302 KVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYV 361
           KVASSALG GPQ AM LAERLYTQGFISYPRTESTAYPSSFDF+  L  L  NP W + V
Sbjct: 301 KVASSALGIGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFRSALAALAHNPLWSNDV 360

Query: 362 RMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYI 421
           R LL  G+ KP+ G D GDHPPITPM LATE+ LG DAWRLYQY+CQHFIG++S DC+Y 
Sbjct: 361 RTLLDTGFVKPKQGHDAGDHPPITPMRLATEEALGTDAWRLYQYICQHFIGTVSPDCRYT 420

Query: 422 RTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNT 481
           RT I+F+  GE F+C G+RVT KGFTSIMPWLAV+E  +P + KG+ + + +VD+Y+G+T
Sbjct: 421 RTSIEFTSGGETFHCVGNRVTSKGFTSIMPWLAVSENNIPAYKKGDAVSIHKVDIYEGST 480

Query: 482 TPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGY 541
           TPPDYLSESELISLMEK+GIGTDASIPVH+NNI ERNYVQV +GRRLVPT LG TLIRGY
Sbjct: 481 TPPDYLSESELISLMEKNGIGTDASIPVHVNNICERNYVQVNSGRRLVPTPLGTTLIRGY 540

Query: 542 QCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFE 601
           QCID DLCLPDIR FIE QITL+AKG+ADH  VVQ VLQQF +KYSYFVKKI++MDALFE
Sbjct: 541 QCIDADLCLPDIRRFIEQQITLIAKGEADHLQVVQHVLQQFMKKYSYFVKKIENMDALFE 600

Query: 602 AQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPL 661
           AQFSPLADSGR LSKCGKC RYMK+IS+QP RLYC  CEEVYYLPQ G+IKLYKE+ CPL
Sbjct: 601 AQFSPLADSGRLLSKCGKCARYMKYISTQPMRLYCVTCEEVYYLPQNGSIKLYKEIICPL 660

Query: 662 DNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMC 721
           D FELL++S+ GP+ KSFPLCP+CYNSPPFEGI+ L+GA K +  +G   KG GMPCF+C
Sbjct: 661 DGFELLLFSMVGPDAKSFPLCPFCYNSPPFEGIDKLFGALKLD-DTGKVGKGAGMPCFLC 720

Query: 722 PHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDK 781
            HPTC+ S+ITQGVCACPEC+GTL+LDPVSAP W+L CN+CNC+V LP  AH+ISTT  K
Sbjct: 721 LHPTCKQSMITQGVCACPECTGTLILDPVSAPKWRLYCNRCNCIVLLPHAAHKISTTDKK 780

Query: 782 CADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGR 841
           C  C+STIIEVDFNKKTTPL+DGATL  GCILCDE LHSL+EMKHGKSF   RG RGRGR
Sbjct: 781 CPTCESTIIEVDFNKKTTPLKDGATLHEGCILCDELLHSLIEMKHGKSFFMRRG-RGRGR 840

Query: 842 GRGRGRG-GRG-RSSGKPMDPKMSFRDF 868
           GRGRGRG  RG R S +  DPKMSFRDF
Sbjct: 841 GRGRGRGSSRGRRGSSRHDDPKMSFRDF 866

BLAST of CSPI03G15690 vs. ExPASy Swiss-Prot
Match: Q9Z321 (DNA topoisomerase 3-beta-1 OS=Mus musculus OX=10090 GN=Top3b PE=1 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 2.9e-266
Identity = 462/869 (53.16%), Postives = 586/869 (67.43%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRK---GSTEVHEFDGRFLGHHAYYKVTSVIGHVF 64
           TVLMVAEKPS+A SIA  LS G MS+ K   G+  VH++ G F G   ++K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVM 62

Query: 65  SIDFQAGFQDWKSTDPLDLF-QASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 124
           ++DF   +  W   DP +LF QA   K E+NPK ++ + L  E RGCD +VLWLDCD+EG
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEG 122

Query: 125 ENICFEVIECT----GFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDAR 184
           ENICFEV++          NG  K V+RARFSS+T+ DI  A+  L EP+ NEAL+VDAR
Sbjct: 123 ENICFEVLDAVLPVMNNAHNG-EKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDAR 182

Query: 185 QEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFW 244
           QE+DL++G AFTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +FKPE +W
Sbjct: 183 QELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYW 242

Query: 245 SVCPYILQTGYE-LNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGL 304
            +   +     E L LDW+R ++FD ++  MF      +  A V   S K++ K RP  L
Sbjct: 243 VLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKAKQRPLAL 302

Query: 305 NTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNP 364
           NTV +L+VASSALG GPQ AMQ+AERLYTQG+ISYPRTE+T YP +FD KG+L     +P
Sbjct: 303 NTVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHP 362

Query: 365 EWGDYVRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLS 424
            W D V+ LL++G ++PR G D GDHPPITPM  ATE  LG DAWRLY+Y+ +HFI ++S
Sbjct: 363 YWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 422

Query: 425 SDCKYIRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVN-EKKLPQFLKGEKIEVARV 484
            DCKY+++ I F I  E F C G  V   GFT IMPW +V  E+ LP   KG+   V  V
Sbjct: 423 HDCKYLQSTISFRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGDTFTVGEV 482

Query: 485 DLYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLG 544
            + +  T+PPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRRL PTNLG
Sbjct: 483 KMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLG 542

Query: 545 ITLIRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKID 604
           I L+ GY  ID +L LP IRS +E Q+ L+A+G+AD+  V+   L  F++K+ YFV  I 
Sbjct: 543 IVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIA 602

Query: 605 HMDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLY 664
            MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+C  C+E Y LPQ GTIKLY
Sbjct: 603 GMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLY 662

Query: 665 KELTCPLDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGM 724
           KEL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF  +                 KGM
Sbjct: 663 KELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRDM----------------KKGM 722

Query: 725 GMPCFMCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAH 784
           G  C  C HPTC+HSL   G+  C EC +G LVLDP S P WK+ CN CN +    + AH
Sbjct: 723 G--CNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAH 782

Query: 785 RISTTRDKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKS-FIR 844
           R+  + D C  C++ +++VDFNK  +PL    T   GCI CD     L+E+KH  S    
Sbjct: 783 RVRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKHAASCHPM 842

Query: 845 NRGSRGRGRGRGRGRGGRGRSSGKPMDPK 862
           +RG  GR +GRGRGRG R      P  PK
Sbjct: 843 HRGGPGRRQGRGRGRGRRPPGKPNPRRPK 851

BLAST of CSPI03G15690 vs. ExPASy Swiss-Prot
Match: O95985 (DNA topoisomerase 3-beta-1 OS=Homo sapiens OX=9606 GN=TOP3B PE=1 SV=1)

HSP 1 Score: 917.1 bits (2369), Expect = 1.4e-265
Identity = 456/868 (52.53%), Postives = 587/868 (67.63%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRK---GSTEVHEFDGRFLGHHAYYKVTSVIGHVF 64
           TVLMVAEKPS+A SIA  LS G +S+ K   G+  VHE+ G F G    +K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVM 62

Query: 65  SIDFQAGFQDWKSTDPLDLF-QASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 124
           ++DF   +  W   DP +LF QA   K E+NPK ++ + L  E RGCD +VLWLDCD+EG
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEG 122

Query: 125 ENICFEVIEC---TGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQ 184
           ENICFEV++         +G  K V+RARFSS+T+ DI  A+  L EP+ NEAL+VDARQ
Sbjct: 123 ENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQ 182

Query: 185 EIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWS 244
           E+DL++G AFTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +FKPE +W 
Sbjct: 183 ELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWV 242

Query: 245 VCPYI-LQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLN 304
           +   +       L LDW+R ++FD ++  MF      +  A+V   S K++ K RP  LN
Sbjct: 243 LQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALN 302

Query: 305 TVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPE 364
           TV +L+VASS+LG GPQ AMQ AERLYTQG+ISYPRTE+T YP +FD KG+L     +P 
Sbjct: 303 TVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPY 362

Query: 365 WGDYVRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSS 424
           W D V+ LL++G ++PR G D GDHPPITPM  ATE  LG DAWRLY+Y+ +HFI ++S 
Sbjct: 363 WADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSH 422

Query: 425 DCKYIRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVN-EKKLPQFLKGEKIEVARVD 484
           DCKY+++ I F I  E+F C+G  V   GFT +MPW +V  E+ LP   +G+   V  V 
Sbjct: 423 DCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVK 482

Query: 485 LYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGI 544
           + +  T PPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRRL PTNLGI
Sbjct: 483 MLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGI 542

Query: 545 TLIRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDH 604
            L+ GY  ID +L LP IRS +E Q+ L+A+G+AD+  V+   L  F++K+ YFV  I  
Sbjct: 543 VLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAG 602

Query: 605 MDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYK 664
           MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+C  C+E Y LPQ GTIKLYK
Sbjct: 603 MDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYK 662

Query: 665 ELTCPLDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMG 724
           EL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF  +                 KGMG
Sbjct: 663 ELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRDM----------------KKGMG 722

Query: 725 MPCFMCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHR 784
             C  C HP+C+HSL   G+  C EC SG LVLDP S P WK+ CNKCN +    + AHR
Sbjct: 723 --CNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHR 782

Query: 785 ISTTRDKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKS-FIRN 844
           +  + D C+ C++ +++VDFNK  +PL    T  +GC+ CD     L+E+KH  S    +
Sbjct: 783 VRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMH 842

Query: 845 RGSRGRGRGRGRGRGGRGRSSGKPMDPK 862
           RG  GR +GRGRGR  R      P  PK
Sbjct: 843 RGGPGRRQGRGRGRARRPPGKPNPRRPK 851

BLAST of CSPI03G15690 vs. ExPASy Swiss-Prot
Match: O96651 (DNA topoisomerase 3-beta OS=Drosophila melanogaster OX=7227 GN=Top3beta PE=2 SV=2)

HSP 1 Score: 825.5 bits (2131), Expect = 5.7e-238
Identity = 414/855 (48.42%), Postives = 557/855 (65.15%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKGS---TEVHEFDGRFLGH-HAYYKVTSVIGHV 64
           +VLMVAEKPS+A S+A  LS+G+ + ++G+      HE+ G F      ++++TSV GHV
Sbjct: 3   SVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCGHV 62

Query: 65  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 124
            S+DF   +  W   DP+ LF  +  K E+NPK ++ + L+ EARGCD LVLWLDCD+EG
Sbjct: 63  MSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEG 122

Query: 125 ENICFEVIECTGFGGNG--TRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQE 184
           ENICFEV++      N   + +  YRA FS++TEKDI KA++ L  PN NEA +VDARQE
Sbjct: 123 ENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQE 182

Query: 185 IDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSV 244
           +DL++G AFTRFQT FFQ +YG+LDS LISYGPCQTPTLGFCV+RH  I TFKPE FW +
Sbjct: 183 LDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHL 242

Query: 245 CPYILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTV 304
              +L    E+ L+W RG++F  D+  M   RV E   A V  ++ K+  K +P  LNTV
Sbjct: 243 --QLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTV 302

Query: 305 NLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWG 364
            L+++ SS LG GP  AMQ+AERLYTQG+ISYPRTE+  YP++FD    LH L  + ++G
Sbjct: 303 ELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFG 362

Query: 365 DYVRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDC 424
           +  R +L D    PR G D GDHPPITPM L       RD WR+Y+++C+HF+G++S D 
Sbjct: 363 EEARSILGD-IQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSRDL 422

Query: 425 KYIRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAV-NEKKLPQFLKGEKIEVARVDLY 484
           KY  T  + S+  E F+CT S +   GFT +M W A   ++  P F++G ++ +  V L 
Sbjct: 423 KYRVTTAKLSVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVRLI 482

Query: 485 QGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITL 544
           +  T PPDYL+ESELI+LME+HGIGTDASIPVHINNI +RNYV ++ GR+L+PT LGI L
Sbjct: 483 ESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVL 542

Query: 545 IRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMD 604
           + GYQ IDP+L LP +R+ +E  +TL+A+G A+   V++  ++ F+ K+ YFVK ID MD
Sbjct: 543 VHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMD 602

Query: 605 ALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKEL 664
           ALFE  FSPLA+SG+A S+CGKC RYMK+I ++P RL+C  C+E Y LP  G +K+Y+E 
Sbjct: 603 ALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALP-IGNVKVYREF 662

Query: 665 TCPLDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMP 724
            CPLD+F+LL +S  G +G+S+P CPYCYN PPF  +  L G                  
Sbjct: 663 KCPLDDFDLLAFS-TGVKGRSYPFCPYCYNHPPFSDMPHLGG------------------ 722

Query: 725 CFMCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRIS 784
           C  C +  C HSL T G+ +C EC +G LVLD   AP WKL CN+C+ ++   KGA +I+
Sbjct: 723 CNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKIT 782

Query: 785 TTRDKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS 844
               KC +C +  + V +    +  +DG+    GCI C      L+E KH     R   S
Sbjct: 783 VEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVE-KHRAVASRPVRS 833

Query: 845 RGRGRGRGRGRGGRG 852
            G  RG   GRGG G
Sbjct: 843 GGGFRGGKAGRGGGG 833

BLAST of CSPI03G15690 vs. ExPASy TrEMBL
Match: A0A0A0L9C7 (DNA topoisomerase OS=Cucumis sativus OX=3659 GN=Csa_3G172970 PE=3 SV=1)

HSP 1 Score: 1801.6 bits (4665), Expect = 0.0e+00
Identity = 867/867 (100.00%), Postives = 867/867 (100.00%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV
Sbjct: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM
Sbjct: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840

Query: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGRGGRGRSSGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 867

BLAST of CSPI03G15690 vs. ExPASy TrEMBL
Match: A0A1S3AYH7 (DNA topoisomerase OS=Cucumis melo OX=3656 GN=LOC103483959 PE=3 SV=1)

HSP 1 Score: 1756.9 bits (4549), Expect = 0.0e+00
Identity = 847/869 (97.47%), Postives = 857/869 (98.62%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVA TVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDFQAGFQDWK+T+PLDLFQASVIKTESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMC ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVV+SVLQQF+QKYSYFVKKID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGASK NPSSGTSSKGMGMPCF+
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT D
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS--RG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SFIRNR S  RG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGRGRGGRGR+SGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGGRGRNSGKPMDPKMSFRDF 869

BLAST of CSPI03G15690 vs. ExPASy TrEMBL
Match: A0A5A7VKD3 (DNA topoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold70G00170 PE=3 SV=1)

HSP 1 Score: 1756.1 bits (4547), Expect = 0.0e+00
Identity = 847/871 (97.24%), Postives = 857/871 (98.39%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVA TVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDFQAGFQDWK+T+PLDLFQASVIKTESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMC ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVV+SVLQQF+QKYSYFVKKID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGASK NPSSGTSSKGMGMPCF+
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT D
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS---- 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SFIRNR S    
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGRGRGRGGRGR+SGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGRGGRGRNSGKPMDPKMSFRDF 871

BLAST of CSPI03G15690 vs. ExPASy TrEMBL
Match: A0A6J1IRJ0 (DNA topoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111479340 PE=3 SV=1)

HSP 1 Score: 1697.2 bits (4394), Expect = 0.0e+00
Identity = 813/867 (93.77%), Postives = 840/867 (96.89%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVA +VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHA+YKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDF A FQDWK+TDPLDLFQASVIKTESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNENRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERG+LFDCDV  MFQKRV+EDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGS V VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVLVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QIT VAKGQADH SVVQ+VLQQF+QKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQ+G IKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI+SLPGPEGKSFPLCPYCYNSPPFEGI+ LYGA+K NPSSG SSKGMGMPCF+
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDALYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLP+GAHRI+TT +
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSF R RGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGR GRGRSSGK MDPKMSFRDF
Sbjct: 841 RGRGRGR-GRGRSSGKLMDPKMSFRDF 866

BLAST of CSPI03G15690 vs. ExPASy TrEMBL
Match: A0A6J1GWS0 (DNA topoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111457562 PE=3 SV=1)

HSP 1 Score: 1696.0 bits (4391), Expect = 0.0e+00
Identity = 812/867 (93.66%), Postives = 841/867 (97.00%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVA +VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHA+YKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDF A FQDWK+TDPLDLFQASVIKTESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERG+LFDCDV  MFQKRV+EDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VR+LLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRVLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           +RTKIQFSIAGEIFNCTGS V VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 LRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QIT VAKGQADH SVVQ+VLQQF+QKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQ+G IKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI+SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGA+K NPSSG SSKGMGMPCF+
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLP+GAHRI+TT +
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSF R RGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGR GRGRSSGK MDPKMSFRDF
Sbjct: 841 RGRGRGR-GRGRSSGKLMDPKMSFRDF 866

BLAST of CSPI03G15690 vs. NCBI nr
Match: XP_004147675.1 (DNA topoisomerase 3-beta isoform X1 [Cucumis sativus] >KGN57242.1 hypothetical protein Csa_011650 [Cucumis sativus])

HSP 1 Score: 1801.6 bits (4665), Expect = 0.0e+00
Identity = 867/867 (100.00%), Postives = 867/867 (100.00%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV
Sbjct: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM
Sbjct: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840

Query: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGRGGRGRSSGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 867

BLAST of CSPI03G15690 vs. NCBI nr
Match: XP_008439059.1 (PREDICTED: DNA topoisomerase 3-beta [Cucumis melo])

HSP 1 Score: 1756.9 bits (4549), Expect = 0.0e+00
Identity = 847/869 (97.47%), Postives = 857/869 (98.62%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVA TVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDFQAGFQDWK+T+PLDLFQASVIKTESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMC ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVV+SVLQQF+QKYSYFVKKID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGASK NPSSGTSSKGMGMPCF+
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT D
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS--RG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SFIRNR S  RG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGRGRGGRGR+SGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGGRGRNSGKPMDPKMSFRDF 869

BLAST of CSPI03G15690 vs. NCBI nr
Match: KAA0067650.1 (DNA topoisomerase 3-beta [Cucumis melo var. makuwa])

HSP 1 Score: 1756.1 bits (4547), Expect = 0.0e+00
Identity = 847/871 (97.24%), Postives = 857/871 (98.39%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVA TVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDFQAGFQDWK+T+PLDLFQASVIKTESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMC ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVV+SVLQQF+QKYSYFVKKID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGASK NPSSGTSSKGMGMPCF+
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT D
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS---- 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SFIRNR S    
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGRGRGRGGRGR+SGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGRGGRGRNSGKPMDPKMSFRDF 871

BLAST of CSPI03G15690 vs. NCBI nr
Match: XP_038891105.1 (DNA topoisomerase 3-beta isoform X1 [Benincasa hispida])

HSP 1 Score: 1718.4 bits (4449), Expect = 0.0e+00
Identity = 824/867 (95.04%), Postives = 843/867 (97.23%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVA TVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHA+YKVTSVIGHV
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDF AGFQDWKSTDPLDLFQASVIKTESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPAGFQDWKSTDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PN+NEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNKNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTF PEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFTPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQ+AMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTL+KN EWGDY
Sbjct: 301 LKVASSALGFGPQIAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLMKNSEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGE+FNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGELFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRL PTNLGITL+RG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLAPTNLGITLVRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVVQ+VLQQF+QKY YFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYCYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGAIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI+SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGA+K NPSSG SSKGMGMPCF+
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           C HPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRIST  +
Sbjct: 721 CLHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTRNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKS  R RGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSLFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRG  GRGR+SG+PMDPKMSFRDF
Sbjct: 841 RGRGRGGRGRGRNSGRPMDPKMSFRDF 867

BLAST of CSPI03G15690 vs. NCBI nr
Match: XP_023528530.1 (DNA topoisomerase 3-beta isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1698.3 bits (4397), Expect = 0.0e+00
Identity = 814/868 (93.78%), Postives = 841/868 (96.89%), Query Frame = 0

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVA +VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHA+YKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDF A FQDWK+TDPLDLFQASVIK ESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERG+LFDCDV  MFQKRV+EDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGS V VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QIT VAKGQADH SVVQ+VLQQF+QKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQ+G IKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI+SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGA+K NPSSG SSKGMGMPCF+
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLP+GAHRI+TT +
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSF R RGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRG-GRGRSSGKPMDPKMSFRDF 868
           RGRGRGRG GRGRSSGK MDPKMSFRDF
Sbjct: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868

BLAST of CSPI03G15690 vs. TAIR 10
Match: AT2G32000.1 (DNA topoisomerase, type IA, core )

HSP 1 Score: 1403.7 bits (3632), Expect = 0.0e+00
Identity = 664/862 (77.03%), Postives = 751/862 (87.12%), Query Frame = 0

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GSTEVHEFDG F G  A+Y+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  QAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENICF 125
              +Q+W + DP DLF A +IK ESNPKAHIC+HLS EARGC  +VLWLDCDREGENICF
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 EVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKVGV 185
           EVIE TGF    +++KVYRARFSSVTEKDI KA+DNLVEPNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 AFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYILQT 245
           AF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI + 
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKVAS 305
           GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRMLL 365
           SALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL   V NP WG YV+ LL
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTPPD 485
           + SI GE F+CTG RVT KGFT+IMPW AV+EKKLP FLKGE+IEV RV+LY+GNT PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQFS 605
           PDLCLPDIRSFIE QITLVAKGQADHS VVQ V+QQFR+K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFE 665
           PLADSGRALSKCGKCLRYMK I++ P RL+CG CEEVYYLPQKGT+KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPHPT 725
           L+IYS+PGPEGKSFPLCPYCYNSPPFEGI+TL+GASK   +   +  G GMPC +CPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCADC 785
           C+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TT ++C +C
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF+R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGGRGRSSGKPMDPKMSFRDF 868
           GRGGR  S  K +DPKMSFRDF
Sbjct: 847 GRGGRRGS--KSVDPKMSFRDF 865

BLAST of CSPI03G15690 vs. TAIR 10
Match: AT2G32000.2 (DNA topoisomerase, type IA, core )

HSP 1 Score: 1311.6 bits (3393), Expect = 0.0e+00
Identity = 617/812 (75.99%), Postives = 702/812 (86.45%), Query Frame = 0

Query: 59  HVFSI---DFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLD 118
           H+ S+   DF   +Q+W + DP DLF A +IK ESNPKAHIC+HLS EARGC  +VLWLD
Sbjct: 30  HLLSVMFSDFPEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLD 89

Query: 119 CDREGENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDA 178
           CDREGENICFEVIE TGF    +++KVYRARFSSVTEKDI KA+DNLVEPNR+EALAVDA
Sbjct: 90  CDREGENICFEVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDA 149

Query: 179 RQEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKF 238
           RQEIDLKVGVAF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKF
Sbjct: 150 RQEIDLKVGVAFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKF 209

Query: 239 WSVCPYILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGL 298
           W++ PYI + GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GL
Sbjct: 210 WALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGL 269

Query: 299 NTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNP 358
           NTVNLLKVASSALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL   V NP
Sbjct: 270 NTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNP 329

Query: 359 EWGDYVRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLS 418
            WG YV+ LLSDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S
Sbjct: 330 VWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVS 389

Query: 419 SDCKYIRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVD 478
            +CKYIRTK++ SI GE F+CTG RVT KGFT+IMPW AV+EKKLP FLKGE+IEV RV+
Sbjct: 390 PNCKYIRTKVELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVE 449

Query: 479 LYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGI 538
           LY+GNT PPDYL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGI
Sbjct: 450 LYEGNTAPPDYLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGI 509

Query: 539 TLIRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDH 598
           TLIRGYQCIDPDLCLPDIRSFIE QITLVAKGQADHS VVQ V+QQFR+K+SYFV++I+H
Sbjct: 510 TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEH 569

Query: 599 MDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYK 658
           MDALFEAQFSPLADSGRALSKCGKCLRYMK I++ P RL+CG CEEVYYLPQKGT+KLYK
Sbjct: 570 MDALFEAQFSPLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYK 629

Query: 659 ELTCPLDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMG 718
           ELTCPLDNFEL+IYS+PGPEGKSFPLCPYCYNSPPFEGI+TL+GASK   +   +  G G
Sbjct: 630 ELTCPLDNFELVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAG 689

Query: 719 MPCFMCPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRI 778
           MPC +CPHPTC+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI
Sbjct: 690 MPCSLCPHPTCQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRI 749

Query: 779 STTRDKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRG 838
           +TT ++C +CDS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF+R RG
Sbjct: 750 TTTSNRCPECDSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RG 809

Query: 839 SRGRGRGRGRGRGGRGRSSGKPMDPKMSFRDF 868
            RGRGRGRGRGRGGR  S  K +DPKMSFRDF
Sbjct: 810 GRGRGRGRGRGRGGRRGS--KSVDPKMSFRDF 838

BLAST of CSPI03G15690 vs. TAIR 10
Match: AT5G63920.1 (topoisomerase 3alpha )

HSP 1 Score: 401.4 bits (1030), Expect = 1.9e-111
Identity = 235/640 (36.72%), Postives = 348/640 (54.37%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKGST---EVHEFDGRFLGHHAYYKVTSVIGHVF 64
           TVL VAEKPS+A S+A  LS G   TR+G +   ++ EFD    G      +TSVIGH+ 
Sbjct: 10  TVLNVAEKPSVAKSVAGILSRGTFRTREGRSRYNKIFEFDYAINGQPCRMLMTSVIGHLM 69

Query: 65  SIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGE 124
            ++F   ++ W S DP DL+QA V+K     K  I + L  EAR  D LVLWLDCDREGE
Sbjct: 70  ELEFADRYRKWHSCDPADLYQAPVMKHVPEDKKDIKKTLEEEARKSDWLVLWLDCDREGE 129

Query: 125 NICFEVIE-CTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 184
           NI FEV++ C     N     + RA FS++ ++DI +A+ NL +PN+  A AVDARQEID
Sbjct: 130 NIAFEVVDVCRAVKHN---LFIRRAHFSALIDRDIHEAVQNLRDPNQLFAEAVDARQEID 189

Query: 185 LKVGVAFTRFQTTFFQGKY-----GNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKF 244
           L++G +FTRFQT   + ++     G   SR+ISYGPCQ PTLGF V+R+ +I   +PE+F
Sbjct: 190 LRIGASFTRFQTMLLRDRFAIDSTGEERSRVISYGPCQFPTLGFIVERYWEIQAHEPEEF 249

Query: 245 WSV-CPYILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQEN-KVRPS 304
           W++ C +  + G     +W RG LFD     +  +  +E+  A V  +   +E  K  P 
Sbjct: 250 WTINCSHQSEEGL-ATFNWMRGHLFDYASAVILYEMCVEEPTATVMNVPHPRERFKYPPY 309

Query: 305 GLNTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVK 364
            LNT+ L K AS       +  M++AE LY  GFISYPRTE+ ++ S  D +  +    +
Sbjct: 310 PLNTIELEKRASRYFRLSSEHTMKVAEELYQAGFISYPRTETDSFSSRTDLRAMVEEQTR 369

Query: 365 NPEWGDYVRMLLSD----GYSKPRSGTDVGDHPPITPMCLAT-EDMLGRDAWRLYQYVCQ 424
           +P WG Y + LL        +    G D   HPPI P   ++ E    RD   +Y+ V +
Sbjct: 370 HPAWGSYAQRLLEPEGGLWRNPANGGHDDKAHPPIHPTKFSSGESNWSRDHLNVYELVVR 429

Query: 425 HFIGSLSSDCKYIRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEK 484
           H++  +S       T ++  IAGE F+ +G  +  K +  +  + +     +P + KG++
Sbjct: 430 HYLACVSQPAVAAETTVEIDIAGERFSASGRAILAKNYLEVYRFESWGGSVIPVYEKGQQ 489

Query: 485 IEVARVDLYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRL 544
                + L    T PP  L E++L+S M+K GIGTDA++  HI  + +R Y    A  R 
Sbjct: 490 FIPTTLTLDAAVTRPPPLLCEADLLSCMDKAGIGTDATMHDHIKKLLDRGYATKDANTRF 549

Query: 545 VPTNLGITLIRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKY-S 604
            PTNLG  L+ GY  +  +L  P++R+ +EH +  V+ G+   + V+++ LQQ +  +  
Sbjct: 550 SPTNLGEALVMGYDDMGYELWKPNLRALMEHDMNEVSVGRKTKAEVLETCLQQMKACFLD 609

Query: 605 YFVKK---IDHMDALFE----AQFSPLADSGRALSKCGKC 621
             VKK   ++ M   FE       S    +G  + +C  C
Sbjct: 610 ARVKKSKLLEAMTIFFERSNNTDESESQTAGEVVRRCNLC 645