CSPI03G14900 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G14900
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDNA-directed RNA polymerase subunit
LocationChr3: 11082599 .. 11084374 (+)
RNA-Seq ExpressionCSPI03G14900
SyntenyCSPI03G14900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTACCATGAAGTTTTGCCGGGAATGGTATGCATTATCATAACCACACTCTCCTCTACTTGCGCTTTCGTTCCTATGAAGTTTCCCCCCGAAAATGTTGTAACCATTGATATTGTTATGTTGCAGTAACAACATTCTCTATCCGAAGGAGGATAGAGATGAGAAGATTCTTCTCTATGCCTGTCGCAACTGTGATCACCAGGTACCATTATTCTCTTTCTTTTGCATATTTCAATTTGTTTTGAAATGTGAACTTTTTTACTTTGTAGTAGTATTAACCTAGGATAAGAAAGGTTATTTGGGCCTGATTCAAGATTATCCCCTTAAATGCTCGGGCTTGCCTCTTTAGTTCGTCATCCTGTGTTAAAGCTTTCCTTATGGCGTCATGTTATCTCAGTTTTTTAGCCTGCCCTTCTATGAATTTGGATGGACCCCTTTGTTCTTTGGCTTTTATAGTCCACTTTCACATCTATAAATTTCAGTAAGGGAAGATTTATGAACATTATGATTTAACTAGTTATCATCTGATAGTATGAAATGCTAACGAGCCCATAAAGTCCTTTGTTGTAGATATGCATGTACAACTGTAATCATAATTTGAGAAAAATGGTAGAAAGGGCCTCTTGTTTCAATTAATCCAGGAGACGGAGTGTAACCTTTAAAATTTTTTAGGATGATATATATAACTTGTCTAATTCTGAAGGTTCCAAGTTTGATCTAATTTTTCATTGATTTTAGACTGTTACACTGTTATTTCACTCCCGGAGTTCACGAGATTGTCCAAGTTAACTTTCATGAGTTATCTTTGATTCTCCGGTTTCTTCGCATTTGTTTGTGTTCTTAAATTCTTTGGCATGTTAGCTATGGTGGAAAACTGGAAGGTTTTGAGCCTGCGACCAATTGAATTACATACCTTATGTACTTCTATACAGTATATGTTTTCTTGATGGACCGTTGTTTGAACTTCCTCTCTGCTTTTGTGTAACTAATTGAAAGAAATTTTAATTTTTATCTACTGGTAGATAGACCTGCTTTCTGAGAATTGTATTTTAACCAATATAGTAGCCTTTTTTATTTGTTCCTAGGATTGCCACAAAATCACCCCATTGATGGGGGTGGACATATAAGTATCCATAGTTTCCCCCTGCTGTTCTCTCTTCTTTGACTTACTAATCCTCTTCTCTGTGTATGGAATTGTTTTATTTCTAGATATTGATGATTTCTTCCCTTCTTATTCCCAGTTCGTAATCATTCATTTTTTTATATATAAAAATTGTTTACAGGAAGTCGCTGACAATAACTGCGTCTATAGGAATGAGATTCATCATGCTGTAGGAGAACGCACTCAAGTTTTGCAAGATGTTGCTGCAGATCCAACTCTCCCTCGCACTAAAGCTGTCCGATGTGCTCAATGCAACCATGGAGAAGCTGTTTTCTTCCAGGTCCACTTTTTGCTTCCGAACTTTGATTTACAAGGCTTGAGCACGAGCACTCTTTTCCTTTATTCTCTCGATTGTTAACGTCATAGATTTTCAACACATGCTTTATTCTTACAACTCTTTAGTAAGGTTCTCTTGAATGAGAAATATCAACTGTTAGTTTGCTTGTGTAATTGTGTGAAATTCTGTCAAGCTGCTTGGAGGTTTCATCATGTTTATACATGTATTAGCCTTGATGTCATTGGTTGTGTGTGCAGGCCACTGCCAGAGGAGAGGAAGGTATGACATTGTTCTTCGTTTGCTGCAATCCAAATTGTGGCCACAGATGGAGAGAT

mRNA sequence

ATGAGTACCATGAAGTTTTGCCGGGAATGTAACAACATTCTCTATCCGAAGGAGGATAGAGATGAGAAGATTCTTCTCTATGCCTGTCGCAACTGTGATCACCAGGAAGTCGCTGACAATAACTGCGTCTATAGGAATGAGATTCATCATGCTGTAGGAGAACGCACTCAAGTTTTGCAAGATGTTGCTGCAGATCCAACTCTCCCTCGCACTAAAGCTGTCCGATGTGCTCAATGCAACCATGGAGAAGCTGTTTTCTTCCAGGCCACTGCCAGAGGAGAGGAAGGTATGACATTGTTCTTCGTTTGCTGCAATCCAAATTGTGGCCACAGATGGAGAGAT

Coding sequence (CDS)

ATGAGTACCATGAAGTTTTGCCGGGAATGTAACAACATTCTCTATCCGAAGGAGGATAGAGATGAGAAGATTCTTCTCTATGCCTGTCGCAACTGTGATCACCAGGAAGTCGCTGACAATAACTGCGTCTATAGGAATGAGATTCATCATGCTGTAGGAGAACGCACTCAAGTTTTGCAAGATGTTGCTGCAGATCCAACTCTCCCTCGCACTAAAGCTGTCCGATGTGCTCAATGCAACCATGGAGAAGCTGTTTTCTTCCAGGCCACTGCCAGAGGAGAGGAAGGTATGACATTGTTCTTCGTTTGCTGCAATCCAAATTGTGGCCACAGATGGAGAGAT

Protein sequence

MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQDVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Homology
[Close]
BLAST of CSPI03G14900 vs. ExPASy Swiss-Prot
Match: Q6NLH0 (DNA-directed RNA polymerases II, IV and V subunit 9A OS=Arabidopsis thaliana OX=3702 GN=NRPB9A PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 6.3e-60
Identity = 99/114 (86.84%), Postives = 110/114 (96.49%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++KILLYACRNCDHQEVADN+CVYRNE+HH+V ERTQ+L 
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQKILLYACRNCDHQEVADNSCVYRNEVHHSVSERTQILT 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVA+DPTLPRTKAVRC++C H EAVFFQATARGEEGMTLFFVCCNPNCGHRWR+
Sbjct: 61  DVASDPTLPRTKAVRCSKCQHREAVFFQATARGEEGMTLFFVCCNPNCGHRWRE 114

[Close]
BLAST of CSPI03G14900 vs. ExPASy Swiss-Prot
Match: Q8L5V0 (DNA-directed RNA polymerases II, IV and V subunit 9B OS=Arabidopsis thaliana OX=3702 GN=NRPB9B PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 1.1e-59
Identity = 98/114 (85.96%), Postives = 108/114 (94.74%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++ ILLYACRNCDHQE ADNNCVYRNE+HH+V E+TQ+L 
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQSILLYACRNCDHQEAADNNCVYRNEVHHSVSEQTQILS 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVA+DPTLPRTKAVRCA+C HGEAVFFQATARGEEGMTLFFVCCNPNC HRWR+
Sbjct: 61  DVASDPTLPRTKAVRCAKCQHGEAVFFQATARGEEGMTLFFVCCNPNCSHRWRE 114

[Close]
BLAST of CSPI03G14900 vs. ExPASy Swiss-Prot
Match: Q32P73 (DNA-directed RNA polymerase II subunit RPB9 OS=Bos taurus OX=9913 GN=POLR2I PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 5.2e-38
Identity = 66/112 (58.93%), Postives = 90/112 (80.36%), Query Frame = 0 

Query: 4   MKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQDVA 63
           ++FC+ECNN+LYPKED++ +ILLYACRNCD+Q+ ADN+C+Y N+I H V E TQ++ DV+
Sbjct: 14  IRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVS 73

Query: 64  ADPTLPRTKAVRCAQCNHGEAVFFQA-TARGEEGMTLFFVCCNPNCGHRWRD 115
            DPTLPRT+   C +C H EAVFFQ+ +AR E+ M L++VC  P+CGHRW +
Sbjct: 74  QDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125

[Close]
BLAST of CSPI03G14900 vs. ExPASy Swiss-Prot
Match: P36954 (DNA-directed RNA polymerase II subunit RPB9 OS=Homo sapiens OX=9606 GN=POLR2I PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 5.2e-38
Identity = 66/112 (58.93%), Postives = 90/112 (80.36%), Query Frame = 0 

Query: 4   MKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQDVA 63
           ++FC+ECNN+LYPKED++ +ILLYACRNCD+Q+ ADN+C+Y N+I H V E TQ++ DV+
Sbjct: 14  IRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVS 73

Query: 64  ADPTLPRTKAVRCAQCNHGEAVFFQA-TARGEEGMTLFFVCCNPNCGHRWRD 115
            DPTLPRT+   C +C H EAVFFQ+ +AR E+ M L++VC  P+CGHRW +
Sbjct: 74  QDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125

[Close]
BLAST of CSPI03G14900 vs. ExPASy Swiss-Prot
Match: P60898 (DNA-directed RNA polymerase II subunit RPB9 OS=Mus musculus OX=10090 GN=Polr2i PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 5.2e-38
Identity = 66/112 (58.93%), Postives = 90/112 (80.36%), Query Frame = 0 

Query: 4   MKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQDVA 63
           ++FC+ECNN+LYPKED++ +ILLYACRNCD+Q+ ADN+C+Y N+I H V E TQ++ DV+
Sbjct: 14  IRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVS 73

Query: 64  ADPTLPRTKAVRCAQCNHGEAVFFQA-TARGEEGMTLFFVCCNPNCGHRWRD 115
            DPTLPRT+   C +C H EAVFFQ+ +AR E+ M L++VC  P+CGHRW +
Sbjct: 74  QDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE 125

[Close]
BLAST of CSPI03G14900 vs. ExPASy TrEMBL
Match: A0A6J1I879 (DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111470884 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.5e-64
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. ExPASy TrEMBL
Match: A0A5A7U5C7 (DNA-directed RNA polymerase subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001440 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.5e-64
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. ExPASy TrEMBL
Match: A0A0A0L816 (DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_3G166240 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.5e-64
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. ExPASy TrEMBL
Match: A0A6J1CBU6 (DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC111009263 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.5e-64
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. ExPASy TrEMBL
Match: A0A6J1FM50 (DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC111445212 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.5e-64
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. NCBI nr
Match: XP_008438942.1 (PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Cucumis melo] >XP_022137993.1 DNA-directed RNA polymerases II, IV and V subunit 9A isoform X2 [Momordica charantia] >XP_022939240.1 DNA-directed RNA polymerases II, IV and V subunit 9A [Cucurbita moschata] >XP_022972305.1 DNA-directed RNA polymerases II, IV and V subunit 9A [Cucurbita maxima] >XP_022972306.1 DNA-directed RNA polymerases II, IV and V subunit 9A [Cucurbita maxima] >XP_023539282.1 DNA-directed RNA polymerases II, IV and V subunit 9A [Cucurbita pepo subsp. pepo] >XP_023539283.1 DNA-directed RNA polymerases II, IV and V subunit 9A [Cucurbita pepo subsp. pepo] >XP_031737720.1 DNA-directed RNA polymerases II, IV and V subunit 9A [Cucumis sativus] >XP_038880081.1 DNA-directed RNA polymerases II, IV and V subunit 9A [Benincasa hispida] >KAA0049537.1 DNA-directed RNA polymerase, M/15kDa subunit [Cucumis melo var. makuwa] >KAG7029011.1 DNA-directed RNA polymerases II, IV and V subunit 9A [Cucurbita argyrosperma subsp. argyrosperma] >TYK16215.1 DNA-directed RNA polymerase, M/15kDa subunit [Cucumis melo var. makuwa])

HSP 1 Score: 254.2 bits (648), Expect = 5.3e-64
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. NCBI nr
Match: XP_030503042.1 (DNA-directed RNA polymerases II, IV and V subunit 9A [Cannabis sativa] >XP_030503043.1 DNA-directed RNA polymerases II, IV and V subunit 9A [Cannabis sativa] >KAF4356255.1 hypothetical protein F8388_000702 [Cannabis sativa] >KAF4357777.1 hypothetical protein G4B88_025012 [Cannabis sativa])

HSP 1 Score: 253.1 bits (645), Expect = 1.2e-63
Identity = 113/114 (99.12%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHH+VGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHSVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. NCBI nr
Match: XP_024019992.1 (DNA-directed RNA polymerases II, IV and V subunit 9A [Morus notabilis])

HSP 1 Score: 250.8 bits (639), Expect = 5.8e-63
Identity = 111/114 (97.37%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKEDRD+KILLYACRNCDHQEVADNNCVYRNEIHH+VGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDRDQKILLYACRNCDHQEVADNNCVYRNEIHHSVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTK+VRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKSVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. NCBI nr
Match: XP_024963039.1 (DNA-directed RNA polymerases II, IV and V subunit 9A [Cynara cardunculus var. scolymus] >KVH87674.1 DNA-directed RNA polymerase, M/15kDa subunit [Cynara cardunculus var. scolymus])

HSP 1 Score: 249.6 bits (636), Expect = 1.3e-62
Identity = 111/114 (97.37%), Postives = 113/114 (99.12%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+D+KILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDKDQKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTKAVRCAQC HGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKAVRCAQCGHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. NCBI nr
Match: KAA8527386.1 (hypothetical protein F0562_034899 [Nyssa sinensis])

HSP 1 Score: 249.2 bits (635), Expect = 1.7e-62
Identity = 110/114 (96.49%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++KILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVAADPTLPRTK+VRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD
Sbjct: 61  DVAADPTLPRTKSVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 114

[Close]
BLAST of CSPI03G14900 vs. TAIR 10
Match: AT3G16980.1 (RNA polymerases M/15 Kd subunit )

HSP 1 Score: 231.1 bits (588), Expect = 4.4e-61
Identity = 99/114 (86.84%), Postives = 110/114 (96.49%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++KILLYACRNCDHQEVADN+CVYRNE+HH+V ERTQ+L 
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQKILLYACRNCDHQEVADNSCVYRNEVHHSVSERTQILT 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVA+DPTLPRTKAVRC++C H EAVFFQATARGEEGMTLFFVCCNPNCGHRWR+
Sbjct: 61  DVASDPTLPRTKAVRCSKCQHREAVFFQATARGEEGMTLFFVCCNPNCGHRWRE 114

[Close]
BLAST of CSPI03G14900 vs. TAIR 10
Match: AT4G16265.1 (RNA polymerases M/15 Kd subunit )

HSP 1 Score: 230.3 bits (586), Expect = 7.6e-61
Identity = 98/114 (85.96%), Postives = 108/114 (94.74%), Query Frame = 0 

Query: 1   MSTMKFCRECNNILYPKEDRDEKILLYACRNCDHQEVADNNCVYRNEIHHAVGERTQVLQ 60
           MSTMKFCRECNNILYPKED+++ ILLYACRNCDHQE ADNNCVYRNE+HH+V E+TQ+L 
Sbjct: 1   MSTMKFCRECNNILYPKEDKEQSILLYACRNCDHQEAADNNCVYRNEVHHSVSEQTQILS 60

Query: 61  DVAADPTLPRTKAVRCAQCNHGEAVFFQATARGEEGMTLFFVCCNPNCGHRWRD 115
           DVA+DPTLPRTKAVRCA+C HGEAVFFQATARGEEGMTLFFVCCNPNC HRWR+
Sbjct: 61  DVASDPTLPRTKAVRCAKCQHGEAVFFQATARGEEGMTLFFVCCNPNCSHRWRE 114

The following BLAST results are available for this feature:
BLAST of CSPI03G14900 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q6NLH06.3e-6086.84DNA-directed RNA polymerases II, IV and V subunit 9A OS=Arabidopsis thaliana OX=... [more]
Q8L5V01.1e-5985.96DNA-directed RNA polymerases II, IV and V subunit 9B OS=Arabidopsis thaliana OX=... [more]
Q32P735.2e-3858.93DNA-directed RNA polymerase II subunit RPB9 OS=Bos taurus OX=9913 GN=POLR2I PE=1... [more]
P369545.2e-3858.93DNA-directed RNA polymerase II subunit RPB9 OS=Homo sapiens OX=9606 GN=POLR2I PE... [more]
P608985.2e-3858.93DNA-directed RNA polymerase II subunit RPB9 OS=Mus musculus OX=10090 GN=Polr2i P... [more]
BLAST of CSPI03G14900 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1I8792.5e-64100.00DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111470884 ... [more]
A0A5A7U5C72.5e-64100.00DNA-directed RNA polymerase subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A0A0L8162.5e-64100.00DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_3G166240 P... [more]
A0A6J1CBU62.5e-64100.00DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC1110092... [more]
A0A6J1FM502.5e-64100.00DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC11144521... [more]
BLAST of CSPI03G14900 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008438942.15.3e-64100.00PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 9A [Cucumis melo] >... [more]
XP_030503042.11.2e-6399.12DNA-directed RNA polymerases II, IV and V subunit 9A [Cannabis sativa] >XP_03050... [more]
XP_024019992.15.8e-6397.37DNA-directed RNA polymerases II, IV and V subunit 9A [Morus notabilis][more]
XP_024963039.11.3e-6297.37DNA-directed RNA polymerases II, IV and V subunit 9A [Cynara cardunculus var. sc... [more]
KAA8527386.11.7e-6296.49hypothetical protein F0562_034899 [Nyssa sinensis][more]
BLAST of CSPI03G14900 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G16980.14.4e-6186.84RNA polymerases M/15 Kd subunit [more]
AT4G16265.17.6e-6185.96RNA polymerases M/15 Kd subunit [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001222Zinc finger, TFIIS-typeSMARTSM00440Cys4_2coord: 74..114
e-value: 2.3E-6
score: 37.2
IPR001222Zinc finger, TFIIS-typePFAMPF01096TFIIS_Ccoord: 75..113
e-value: 5.6E-11
score: 42.1
IPR001222Zinc finger, TFIIS-typePROSITEPS51133ZF_TFIIS_2coord: 72..113
score: 12.77014
IPR001529DNA-directed RNA polymerase, M/15kDa subunitSMARTSM00661rpol9cneucoord: 5..58
e-value: 7.7E-16
score: 68.6
IPR001529DNA-directed RNA polymerase, M/15kDa subunitPFAMPF02150RNA_POL_M_15KDcoord: 4..36
e-value: 3.3E-11
score: 42.8
IPR012164DNA-directed RNA polymerase subunit/transcription factor SPIRSFPIRSF005586RNApol_RpoMcoord: 4..114
e-value: 5.9E-20
score: 69.8
IPR012164DNA-directed RNA polymerase subunit/transcription factor SPANTHERPTHR11239DNA-DIRECTED RNA POLYMERASEcoord: 1..114
NoneNo IPR availableGENE3D2.20.25.10coord: 1..46
e-value: 4.5E-26
score: 92.2
NoneNo IPR availableGENE3D2.20.25.10coord: 47..114
e-value: 3.4E-30
score: 105.7
NoneNo IPR availablePANTHERPTHR11239:SF17DNA-DIRECTED RNA POLYMERASE SUBUNITcoord: 1..114
NoneNo IPR availableSUPERFAMILY57783Zinc beta-ribboncoord: 52..114
NoneNo IPR availableSUPERFAMILY57783Zinc beta-ribboncoord: 1..48
IPR019761DNA-directed RNA polymerase M, 15kDa subunit, conserved sitePROSITEPS01030RNA_POL_M_15KDcoord: 6..32
IPR034012Pol II subunit B9, C-terminal zinc ribbonCDDcd10508Zn-ribbon_RPB9coord: 64..113
e-value: 1.96154E-21
score: 78.0638

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G14900.1CSPI03G14900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001193 maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter
biological_process GO:0006379 mRNA cleavage
biological_process GO:0006283 transcription-coupled nucleotide-excision repair
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005730 nucleolus
cellular_component GO:0005665 RNA polymerase II, core complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding