CSPI03G12690 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G12690
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSulfate adenylyltransferase
LocationChr3: 9661008 .. 9664176 (-)
RNA-Seq ExpressionCSPI03G12690
SyntenyCSPI03G12690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGAAAGAGAGCAATGACGACGGGGGAAATGGGTGTCTAGGTTCATCCTCCCAACTAAAGGAGAAAAATTTATTCATTCCCCTCTCATACACAACGAATCTTCATTCCACTTTCAATTCTATTTTATCTCTAACTAAGTTGAATCATTATAAATTTATATCCCATCCCACTAACCCAAAAAAAAAAAAAAAGGGTTGCGAAATCCCCCTCTCCGATTCTCGCTTTTGGCTCTCTTTTTCTCTCCCACCTTTTTACCTCTCCCATTTCGTCCTCTTCCCTAATTACTCTCAGCTTTGAAGCCTTGGTCTCCATCTCAGATGGCGTCCATGGCGACTCGTTTCACCAATACCCCATCCCCATTTCATTCTATTCAGAGAACTTCTTACACCCATTTGGGCGCTCCGATTAAGGTCTCCATTGCTACCTCCAAGTCGAAGAAGACCCATCTCCGCCTCCGGGTTTCTGCTGGTTTGATCGAACCGGACGGCGGCAAGCTGGTGGAGCTTCTTGTTGAGGAGCCTCTGAGGGGTTTGAAGAAGAAGGAAGCCTTATCCTTGCCAAGGATCGAGCTTTCTAGCATTGATATTCAATGGGTTCATGTTCTCAGCGAAGGATGGGCCAGTCCTCTTACCGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTTAATTCCCTCAGGCTTCCAGATGGGTCTGTGGCCAATATGTCAGTGCCCATTGTTCTGGCCATTGACGATGCTCAAAAGCACAGCATCGGGGACTCCACCAGTGTGGCTCTCTTTGATTCTAAGAACAACCCCATCGCTGTTCTCAAGAAGTAAGATTTCTCTCACTTTGGTTTTCCTCTTTTCATTTCTGAAAGCTTTGTGGTTCTCATGAATCTGTCTATACTGGTAAAACCATTGCGTGGCTTTGTTTCTGATGAATCTATCTCTATGCTATCTATTGCTATGTATTATTCGAGGATTTCTCCCTCTTATTTTCTGTCTGGAAATGAGAGTCTTGTTTTCTTTCCTTTGAACGTCAGTTCTATAACTAGTTGTATCAGAAAAACATCCTAGTTTAATTAAAATTGCTGCTCTGCATAGATGAATCTCACATGTTTGGGATTGTATTTCACATCTTTTGTCTCCTAGGATCATAGCACTCATTGATGGCATCATCAAAATCTTGTGTAACATGTACTTTCTTGAGACCAGATTGTTATACTTATACCCGTTGAACTGATTCACGCTATGCTAAATGTCTAATAGTAGAGTAGCAAAGACTTCCCTGTTGAAATCATTTCAAAGCGTCTAATGAAGTCTTCCAATTTCTTGTATGGATGTATCAGTATTGAGATCTACAAGCACCCTAAAGAAGAAAGGATAGCAAGAACCTGGGGCACCACCGCCCCGGGGCTCCCTTATGTTGATCAAGCTATAACCAATGCTGGTAATTGGCTGATCGGAGGTGACTTAGAGGTTATAGAGCCAATCAAGTACCATGATGGCCTTGACCGTTTCCGACAGTCACCTGCAGAACTACGGGAGGAATTTACCCGGCGAAATGCAGATGCAGTGTTTGCATTCCAACTTCGAAACCCAGTACATAACGGCCATGCATTACTAATGACTGACACCCGCCGTCGATTGCTTGAGATGGGATACAAGAACCCCATTTTGTTGCTTCATCCTCTGGGTGGCTACACCAAGGCAGATGATGTTCCACTTAGTTGGCGAATGAAGCAACATGAGAAGGTACCTAATTTTAAACCATCTTTCACTTTGTTTGTTGATTGGATATGGATAGCTCTAAAATTTAAGAACTGCGTCTATCCTACCAGGTGCTCGAGGATGGTGTTCTTGATCCAGAGACCACAGTTGTTTCCATATTTCCATCTCCAATGCACTATGCAGGGCCAACTGAAGTGCAGTGGCATGCAAAAGCAAGAATTAATGCAGGAGCCAACTTTTACATTGTTGGACGCGACCCAGCTGGAATGGGTCATCCTACTGAGAAAAGGGATTTGTATGATGCTGACCACGGGAAGAAAGTGTTGAGCATGGCTCCTGGACTAGAGCGGCTAAACATCCTTCCATTCAGAGTAAGTTTATTTAGAATGTCATGGTATCAAACATGGAATTTTAGGTAGAGAGAGTTACTCATCTATTTTATTACACGTTCAGGTTGCTGCTTACGATAAAACTCAGGGCAAGATGGCCTTCTTCGACCCCTCTAGAGCTCAGGATTTCCTTTTCATATCCGGCACCAAGGTAATTTCCTGCTAATACAATAAAATCTAGTCATTGCCACTTTTGATGCTTTAATCTCAATCATCAATTATCCAATTAAACGTCAAAGAAACAAATTATATGATACCATTGAATAGGAAAATTTTCCAGTACTACTGAGAGCCTCCTCTGTTCACTAGAAAATGTTCGTAGTCCAGTGGACTCCACAAGTGTGCCATATACGGAGCCATTTGACTAGAGAATTAAGAATGAGAACTAAGTTAATAGATCAACAGCATATTTATTGTGTAATGACAAAACGTATGTTTGGATGCTGCAGATGAGAACATTAGCGAAGAACAAAGAGAATCCGCCAGAAGGTTTTATGTGCCCAGGTGGATGGAAGGTGTTGGTTGAATATTATGATAGTTTGGTACCTGCAAGCAGTGGCAGACTTCCTGAACCTGAAGCTGTTCTGGCGTAGGCTTAGGGTATTGTATCCGAGAAAAGTATTTAAACTTTTGAAAATACAGTACCAGTGATGAATAAGATAGTGAGGCGATGGTACAGAAGCATTGCATGATTTGCCTCTCTTTGTAAATTAGATGCATTCATCGCATCTTTGGGTTTGGTACAATACAGCATATTGTTTACTAGTGTGCTTTTGTTCAATTTTATGAAGGAGATGAGTTTGCTGAACGTCATCATTATATTATTCAGATAGAATCCTTTTAGTGGGTTTTGTTTCAAGTAGAGATAGGGACGACTGAGCATTGTTGTTCATAGGGGGAACATAAAACATTGAAAGTTAATGGATAATCACATAGTTCTAGGCATGAGATGTAATTTATATCGCCATGGAAATCGGCGGACGGAAATTTAAGGGTTTGATTTTAATTAGATTATACAATTTGGTTTTACAATTTTAATCAATAAAGCTCATCACAGAG

mRNA sequence

AGGAAAGAGAGCAATGACGACGGGGGAAATGGGTGTCTAGGTTCATCCTCCCAACTAAAGGAGAAAAATTTATTCATTCCCCTCTCATACACAACGAATCTTCATTCCACTTTCAATTCTATTTTATCTCTAACTAAGTTGAATCATTATAAATTTATATCCCATCCCACTAACCCAAAAAAAAAAAAAAAGGGTTGCGAAATCCCCCTCTCCGATTCTCGCTTTTGGCTCTCTTTTTCTCTCCCACCTTTTTACCTCTCCCATTTCGTCCTCTTCCCTAATTACTCTCAGCTTTGAAGCCTTGGTCTCCATCTCAGATGGCGTCCATGGCGACTCGTTTCACCAATACCCCATCCCCATTTCATTCTATTCAGAGAACTTCTTACACCCATTTGGGCGCTCCGATTAAGGTCTCCATTGCTACCTCCAAGTCGAAGAAGACCCATCTCCGCCTCCGGGTTTCTGCTGGTTTGATCGAACCGGACGGCGGCAAGCTGGTGGAGCTTCTTGTTGAGGAGCCTCTGAGGGGTTTGAAGAAGAAGGAAGCCTTATCCTTGCCAAGGATCGAGCTTTCTAGCATTGATATTCAATGGGTTCATGTTCTCAGCGAAGGATGGGCCAGTCCTCTTACCGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTTAATTCCCTCAGGCTTCCAGATGGGTCTGTGGCCAATATGTCAGTGCCCATTGTTCTGGCCATTGACGATGCTCAAAAGCACAGCATCGGGGACTCCACCAGTGTGGCTCTCTTTGATTCTAAGAACAACCCCATCGCTGTTCTCAAGAATATTGAGATCTACAAGCACCCTAAAGAAGAAAGGATAGCAAGAACCTGGGGCACCACCGCCCCGGGGCTCCCTTATGTTGATCAAGCTATAACCAATGCTGGTAATTGGCTGATCGGAGGTGACTTAGAGGTTATAGAGCCAATCAAGTACCATGATGGCCTTGACCGTTTCCGACAGTCACCTGCAGAACTACGGGAGGAATTTACCCGGCGAAATGCAGATGCAGTGTTTGCATTCCAACTTCGAAACCCAGTACATAACGGCCATGCATTACTAATGACTGACACCCGCCGTCGATTGCTTGAGATGGGATACAAGAACCCCATTTTGTTGCTTCATCCTCTGGGTGGCTACACCAAGGCAGATGATGTTCCACTTAGTTGGCGAATGAAGCAACATGAGAAGGTGCTCGAGGATGGTGTTCTTGATCCAGAGACCACAGTTGTTTCCATATTTCCATCTCCAATGCACTATGCAGGGCCAACTGAAGTGCAGTGGCATGCAAAAGCAAGAATTAATGCAGGAGCCAACTTTTACATTGTTGGACGCGACCCAGCTGGAATGGGTCATCCTACTGAGAAAAGGGATTTGTATGATGCTGACCACGGGAAGAAAGTGTTGAGCATGGCTCCTGGACTAGAGCGGCTAAACATCCTTCCATTCAGAGTTGCTGCTTACGATAAAACTCAGGGCAAGATGGCCTTCTTCGACCCCTCTAGAGCTCAGGATTTCCTTTTCATATCCGGCACCAAGATGAGAACATTAGCGAAGAACAAAGAGAATCCGCCAGAAGGTTTTATGTGCCCAGGTGGATGGAAGGTGTTGGTTGAATATTATGATAGTTTGGTACCTGCAAGCAGTGGCAGACTTCCTGAACCTGAAGCTGTTCTGGCGTAGGCTTAGGGTATTGTATCCGAGAAAAGTATTTAAACTTTTGAAAATACAGTACCAGTGATGAATAAGATAGTGAGGCGATGGTACAGAAGCATTGCATGATTTGCCTCTCTTTGTAAATTAGATGCATTCATCGCATCTTTGGGTTTGGTACAATACAGCATATTGTTTACTAGTGTGCTTTTGTTCAATTTTATGAAGGAGATGAGTTTGCTGAACGTCATCATTATATTATTCAGATAGAATCCTTTTAGTGGGTTTTGTTTCAAGTAGAGATAGGGACGACTGAGCATTGTTGTTCATAGGGGGAACATAAAACATTGAAAGTTAATGGATAATCACATAGTTCTAGGCATGAGATGTAATTTATATCGCCATGGAAATCGGCGGACGGAAATTTAAGGGTTTGATTTTAATTAGATTATACAATTTGGTTTTACAATTTTAATCAATAAAGCTCATCACAGAG

Coding sequence (CDS)

ATGGCGTCCATGGCGACTCGTTTCACCAATACCCCATCCCCATTTCATTCTATTCAGAGAACTTCTTACACCCATTTGGGCGCTCCGATTAAGGTCTCCATTGCTACCTCCAAGTCGAAGAAGACCCATCTCCGCCTCCGGGTTTCTGCTGGTTTGATCGAACCGGACGGCGGCAAGCTGGTGGAGCTTCTTGTTGAGGAGCCTCTGAGGGGTTTGAAGAAGAAGGAAGCCTTATCCTTGCCAAGGATCGAGCTTTCTAGCATTGATATTCAATGGGTTCATGTTCTCAGCGAAGGATGGGCCAGTCCTCTTACCGGATTCATGAGAGAATCCGAGTTCCTCCAAACGCTTCATTTTAATTCCCTCAGGCTTCCAGATGGGTCTGTGGCCAATATGTCAGTGCCCATTGTTCTGGCCATTGACGATGCTCAAAAGCACAGCATCGGGGACTCCACCAGTGTGGCTCTCTTTGATTCTAAGAACAACCCCATCGCTGTTCTCAAGAATATTGAGATCTACAAGCACCCTAAAGAAGAAAGGATAGCAAGAACCTGGGGCACCACCGCCCCGGGGCTCCCTTATGTTGATCAAGCTATAACCAATGCTGGTAATTGGCTGATCGGAGGTGACTTAGAGGTTATAGAGCCAATCAAGTACCATGATGGCCTTGACCGTTTCCGACAGTCACCTGCAGAACTACGGGAGGAATTTACCCGGCGAAATGCAGATGCAGTGTTTGCATTCCAACTTCGAAACCCAGTACATAACGGCCATGCATTACTAATGACTGACACCCGCCGTCGATTGCTTGAGATGGGATACAAGAACCCCATTTTGTTGCTTCATCCTCTGGGTGGCTACACCAAGGCAGATGATGTTCCACTTAGTTGGCGAATGAAGCAACATGAGAAGGTGCTCGAGGATGGTGTTCTTGATCCAGAGACCACAGTTGTTTCCATATTTCCATCTCCAATGCACTATGCAGGGCCAACTGAAGTGCAGTGGCATGCAAAAGCAAGAATTAATGCAGGAGCCAACTTTTACATTGTTGGACGCGACCCAGCTGGAATGGGTCATCCTACTGAGAAAAGGGATTTGTATGATGCTGACCACGGGAAGAAAGTGTTGAGCATGGCTCCTGGACTAGAGCGGCTAAACATCCTTCCATTCAGAGTTGCTGCTTACGATAAAACTCAGGGCAAGATGGCCTTCTTCGACCCCTCTAGAGCTCAGGATTTCCTTTTCATATCCGGCACCAAGATGAGAACATTAGCGAAGAACAAAGAGAATCCGCCAGAAGGTTTTATGTGCCCAGGTGGATGGAAGGTGTTGGTTGAATATTATGATAGTTTGGTACCTGCAAGCAGTGGCAGACTTCCTGAACCTGAAGCTGTTCTGGCGTAG

Protein sequence

MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKLVELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFNSLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA*
Homology
BLAST of CSPI03G12690 vs. ExPASy Swiss-Prot
Match: Q9LIK9 (ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 6.2e-223
Identity = 376/461 (81.56%), Postives = 406/461 (88.07%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMA   + TP     + ++S  +   P       SKS +  +   + AGLI PDGGKL
Sbjct: 1   MASMAAVLSKTPFLSQPLTKSS-PNSDLPFAAVSFPSKSLRRRVG-SIRAGLIAPDGGKL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VEL+VEEP R  KK EA  LPR+EL++ID+QW+HVLSEGWASPL GFMRESEFLQTLHFN
Sbjct: 61  VELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRL DGSV NMSVPIVLAIDD QK  IG+ST VALF+S  NP+A+L +IEIYKHPKEER
Sbjct: 121 SLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVD+AITNAGNWLIGGDLEV+EP+KY+DGLDRFR SPAELR+E  +R
Sbjct: 181 IARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKELEKR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGG+TKADDVPL WRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
            EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 VEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPE 462
           MRTLAKN ENPP+GFMCPGGWKVLV+YY+SL PA +GRLPE
Sbjct: 421 MRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of CSPI03G12690 vs. ExPASy Swiss-Prot
Match: O23324 (ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 7.4e-216
Identity = 371/463 (80.13%), Postives = 404/463 (87.26%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIA-TSKSKKTHLR-LRVSAGLIEPDGG 60
           MASM+T F   P+ F S Q  + +H    +  SI+  S SK   LR + V AGLIEPDGG
Sbjct: 1   MASMSTVFPK-PTSFIS-QPLTKSHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGG 60

Query: 61  KLVELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH 120
           KLV+L+V EP R  KK EA  LPR+ L++ID+QW+HVLSEGWASPL GFMRESEFLQTLH
Sbjct: 61  KLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTLH 120

Query: 121 FNSLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKE 180
           FN L L DGSV NMSVPIVLAIDD QK  IG+S  V+L DS +NPIA+L +IEIYKHPKE
Sbjct: 121 FNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILNDIEIYKHPKE 180

Query: 181 ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT 240
           ERIARTWGTTAPGLPYV++AITNAG+WLIGGDLEV+EP+KY+DGLDRFR SP ELR+E  
Sbjct: 181 ERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKELE 240

Query: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWR 300
           +R ADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGG+TKADDVPLSWR
Sbjct: 241 KRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSWR 300

Query: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           MKQHEKVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 361 HPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420
           HP EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG
Sbjct: 361 HPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420

Query: 421 TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPE 462
           TKMR LAKN+ENPP+GFMCPGGWKVLV+YYDSL    + +LPE
Sbjct: 421 TKMRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of CSPI03G12690 vs. ExPASy Swiss-Prot
Match: Q9S7D8 (ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.5e-208
Identity = 359/468 (76.71%), Postives = 396/468 (84.62%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLG--APIKVSIATSKSKKTHLRLRVSAGLIEPDGG 60
           MAS A     + SPF S       H    AP  +S+ +   + +   L V +GLIEPDGG
Sbjct: 1   MASSAAAIV-SGSPFRSSPLIHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPDGG 60

Query: 61  KLVELLVEEPLRGLKKKEALSLP-RIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTL 120
           KL+ L+VEE  R + K EA ++P RI+L+ +D++WVHVLSEGWASPL GFMR+SEFLQTL
Sbjct: 61  KLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQTL 120

Query: 121 HFNSLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPK 180
           HFNS RL DGSV NMSVPIVLAIDD QK  IGDS  V L DS  NPIA+L +IEIYKHPK
Sbjct: 121 HFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDIEIYKHPK 180

Query: 181 EERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEF 240
           EERIARTWGTTA GLPY ++AIT AGNWLIGGDL+V+EPIKY+DGLDRFR SP++LREEF
Sbjct: 181 EERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLREEF 240

Query: 241 TRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSW 300
            RR ADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNP+LLL+PLGG+TKADDVPLSW
Sbjct: 241 IRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVPLSW 300

Query: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           RM+QHEKVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFIS 420
           GHPTEKRDLYDADHGKKVLSMAPGLERLNILPF+VAAYDKTQGKMAFFDPSR+QDFLFIS
Sbjct: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFLFIS 420

Query: 421 GTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSL-VPASSGRLPEPEA 465
           GTKMR LAK KENPP+GFMCP GWKVLV+YYDSL     +GR+ E  A
Sbjct: 421 GTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAETGNGRVSEAVA 467

BLAST of CSPI03G12690 vs. ExPASy Swiss-Prot
Match: Q43870 (ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 2.2e-188
Identity = 306/407 (75.18%), Postives = 356/407 (87.47%), Query Frame = 0

Query: 45  RLRVSAGLIEPDGGKLVELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPL 104
           ++ V + LI+PDGG+LVEL+V E   G+KK E+ ++P+++L+ ID++WVHV+SEGWASPL
Sbjct: 57  KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPL 116

Query: 105 TGFMRESEFLQTLHFNSLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPI 164
            GFMRE E+LQ+LHFNSLRL +G+  NMS+PIVLAIDD  K  IG S +VAL   + + I
Sbjct: 117 KGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDII 176

Query: 165 AVLKNIEIYKHPKEERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLD 224
             L+++EIYKH KEERIARTWGTT+PGLPYV++ IT +GNWLIGGDLEV EPIKY+DGLD
Sbjct: 177 GSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLD 236

Query: 225 RFRQSPAELREEFTRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPL 284
            +R SP +LREEF  R ADAVFAFQLRNPVHNGHALLM DTR+RLLEMGYKNP+LLLHPL
Sbjct: 237 HYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPL 296

Query: 285 GGYTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAG 344
           GG+TKADDVPL  RM+QH KVLEDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAG
Sbjct: 297 GGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAG 356

Query: 345 ANFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAF 404
           ANFYIVGRDPAGMGHPTEKRDLYD DHGK+VLSMAPGLE+LNILPFRVAAYD  + KMAF
Sbjct: 357 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAF 416

Query: 405 FDPSRAQDFLFISGTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSL 452
           FDPSRA++FLFISGTKMRT A+  ENPP+GFMCP GW VLV+YY+SL
Sbjct: 417 FDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESL 463

BLAST of CSPI03G12690 vs. ExPASy Swiss-Prot
Match: Q27128 (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase OS=Urechis caupo OX=6431 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.7e-140
Identity = 239/387 (61.76%), Postives = 295/387 (76.23%), Query Frame = 0

Query: 62  ELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFNS 121
           EL V E      K E + LP +E++ +D QWV VLSEGWA+PLTGFMRE E+LQ+ HF  
Sbjct: 221 ELFVPESGLEHAKAEIVDLPTMEITKLDTQWVQVLSEGWATPLTGFMREREYLQSQHFGC 280

Query: 122 LRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEERI 181
           L   DG V N S+PIVL +  A K  +  S++ AL   +   IA+L+  E Y+H KEER 
Sbjct: 281 LL--DGGVTNQSIPIVLPVHTADKDRLEGSSAFAL-SYEGKRIAILRTPEFYEHRKEERC 340

Query: 182 ARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRRN 241
           +R +GT+  G PYV + I  +G+WL+GGDLEV+E I ++DGLD +R +P ELR +F   N
Sbjct: 341 SRQFGTSNAGQPYV-KMIMESGDWLVGGDLEVLERITWNDGLDEYRLTPNELRAKFRALN 400

Query: 242 ADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMKQ 301
           ADAVFAFQLRNPVHNGHALLMTDTRRRL E GYK P+LLLHPLGG+TK DDVPL+WRMKQ
Sbjct: 401 ADAVFAFQLRNPVHNGHALLMTDTRRRLTERGYKKPVLLLHPLGGWTKDDDVPLAWRMKQ 460

Query: 302 HEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPT 361
           H+ +L++ VLDP+ TV++IFPSPM YAGPTEVQWHAKAR++ GANFYIVGRDPAGM HP 
Sbjct: 461 HQAILDEKVLDPDYTVMAIFPSPMMYAGPTEVQWHAKARMSTGANFYIVGRDPAGMPHPE 520

Query: 362 EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTKM 421
            K+DLY+A HG KVL+MAPGL +L I+PFRVAAY+KT+  M F+DP R  +F+FISGTKM
Sbjct: 521 TKQDLYNATHGAKVLTMAPGLTQLEIVPFRVAAYNKTKSAMDFYDPERHDEFMFISGTKM 580

Query: 422 RTLAKNKENPPEGFMCPGGWKVLVEYY 449
           R +A+  E PP GFM P  WK++VEYY
Sbjct: 581 RGMARAGETPPNGFMAPSAWKIMVEYY 603

BLAST of CSPI03G12690 vs. ExPASy TrEMBL
Match: A0A0A0L5D0 (Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G144230 PE=4 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 5.6e-275
Identity = 465/467 (99.57%), Postives = 467/467 (100.00%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMATRFTNTPSPFHSIQRTSYTHLGAP+KVSI+TSKSKKTHLRLRVSAGLIEPDGGKL
Sbjct: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPVKVSISTSKSKKTHLRLRVSAGLIEPDGGKL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 468
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 467

BLAST of CSPI03G12690 vs. ExPASy TrEMBL
Match: A0A1S3AWG3 (Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103483665 PE=4 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 1.8e-273
Identity = 463/467 (99.14%), Postives = 466/467 (99.79%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMATRFT TPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGG+L
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLK+IEIYKHPKEER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQ+ITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQSITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 468
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 467

BLAST of CSPI03G12690 vs. ExPASy TrEMBL
Match: A0A5A7U0F4 (Sulfate adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00860 PE=4 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 5.2e-273
Identity = 462/467 (98.93%), Postives = 465/467 (99.57%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMATRFT TPSPFHSIQRTSYTH GAPIKVSIATSKSKKTHLRLRVSAGLIEPDGG+L
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHFGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLK+IEIYKHPKEER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQ+ITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQSITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 468
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 467

BLAST of CSPI03G12690 vs. ExPASy TrEMBL
Match: A0A6J1FPI4 (Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447315 PE=4 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.3e-259
Identity = 440/469 (93.82%), Postives = 453/469 (96.59%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSI--ATSKSKKTHLRLRVSAGLIEPDGG 60
           MAS ATRF   PSPFHSIQ+TSYTH  APIK+S+   + K+KK +LRLRVSAGLIEPDGG
Sbjct: 1   MASTATRFIKAPSPFHSIQKTSYTH--APIKISLPAGSFKAKKANLRLRVSAGLIEPDGG 60

Query: 61  KLVELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH 120
           +LVELLVEEPLRGLKKKEALSLPRI LSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH
Sbjct: 61  RLVELLVEEPLRGLKKKEALSLPRISLSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH 120

Query: 121 FNSLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKE 180
           FNSLRLPDGSVANMSVPIVLAIDDAQKH IGDSTSVALFD+ NNPIA++K+IEIYKHPKE
Sbjct: 121 FNSLRLPDGSVANMSVPIVLAIDDAQKHHIGDSTSVALFDANNNPIAIMKDIEIYKHPKE 180

Query: 181 ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT 240
           ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT
Sbjct: 181 ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT 240

Query: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWR 300
           RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNP+LLLHPLGGYTKADDVPL WR
Sbjct: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLHPLGGYTKADDVPLGWR 300

Query: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 361 HPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420
           HPT+KRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG
Sbjct: 361 HPTKKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420

Query: 421 TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 468
           TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRL EPEAVLA
Sbjct: 421 TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLAEPEAVLA 467

BLAST of CSPI03G12690 vs. ExPASy TrEMBL
Match: A0A6J1C916 (Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111009330 PE=4 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 432/462 (93.51%), Postives = 445/462 (96.32%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMATRFT + SPFHSI RTS  H  AP+K+SI+ S   +T LRLRVSAGLIEPDGG+L
Sbjct: 1   MASMATRFTKSSSPFHSITRTSNPHFAAPVKISISRSSKARTPLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKK+EALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKREALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKH I DSTSVALFD+ NNPIA+LK+IEIYKHP+EER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIADSTSVALFDANNNPIAILKDIEIYKHPEEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLL+MGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLDMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEP 463
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPAS+ RLPEP
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASNDRLPEP 462

BLAST of CSPI03G12690 vs. NCBI nr
Match: XP_004134113.1 (ATP sulfurylase 1, chloroplastic [Cucumis sativus] >KGN56933.1 hypothetical protein Csa_009913 [Cucumis sativus])

HSP 1 Score: 956.1 bits (2470), Expect = 1.2e-274
Identity = 465/467 (99.57%), Postives = 467/467 (100.00%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMATRFTNTPSPFHSIQRTSYTHLGAP+KVSI+TSKSKKTHLRLRVSAGLIEPDGGKL
Sbjct: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPVKVSISTSKSKKTHLRLRVSAGLIEPDGGKL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 468
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 467

BLAST of CSPI03G12690 vs. NCBI nr
Match: XP_008438607.1 (PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 951.0 bits (2457), Expect = 3.7e-273
Identity = 463/467 (99.14%), Postives = 466/467 (99.79%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMATRFT TPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGG+L
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLK+IEIYKHPKEER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQ+ITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQSITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 468
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 467

BLAST of CSPI03G12690 vs. NCBI nr
Match: KAA0049323.1 (ATP sulfurylase 1 [Cucumis melo var. makuwa] >TYK17235.1 ATP sulfurylase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 949.5 bits (2453), Expect = 1.1e-272
Identity = 462/467 (98.93%), Postives = 465/467 (99.57%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMATRFT TPSPFHSIQRTSYTH GAPIKVSIATSKSKKTHLRLRVSAGLIEPDGG+L
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHFGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLK+IEIYKHPKEER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQ+ITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQSITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 468
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 467

BLAST of CSPI03G12690 vs. NCBI nr
Match: XP_038895326.1 (ATP sulfurylase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 938.3 bits (2424), Expect = 2.5e-269
Identity = 455/467 (97.43%), Postives = 461/467 (98.72%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMATRFT TPSPFHSIQRTSYTHLGAPIKVSIA+SKSKK+H RLRVSAGLIEPDGG+L
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHLGAPIKVSIASSKSKKSHFRLRVSAGLIEPDGGRL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VELLVEEPLR LKKKEALSLPRIELSSIDIQW HVLSEGWASPLTGFMRESEFLQTLHFN
Sbjct: 61  VELLVEEPLRALKKKEALSLPRIELSSIDIQWAHVLSEGWASPLTGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRLPDGSVANMSVPIVLAIDDAQKH IGDSTSVALFD+ NNPIA+LK+IEIYKHPKEER
Sbjct: 121 SLRLPDGSVANMSVPIVLAIDDAQKHRIGDSTSVALFDANNNPIAILKDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR
Sbjct: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
           TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK
Sbjct: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 468
           MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA
Sbjct: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 467

BLAST of CSPI03G12690 vs. NCBI nr
Match: XP_023539233.1 (ATP sulfurylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 913.7 bits (2360), Expect = 6.6e-262
Identity = 443/469 (94.46%), Postives = 455/469 (97.01%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSI--ATSKSKKTHLRLRVSAGLIEPDGG 60
           MAS ATRF   PSPFHSIQRTSYTHLGAPIK+S+   + K+KK +LRLRVSAGLIEPDGG
Sbjct: 1   MASTATRFIKAPSPFHSIQRTSYTHLGAPIKISLPAGSFKAKKANLRLRVSAGLIEPDGG 60

Query: 61  KLVELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH 120
           +LVELLVEEPLRGLKKKEALSLPRI LSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH
Sbjct: 61  RLVELLVEEPLRGLKKKEALSLPRISLSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH 120

Query: 121 FNSLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKE 180
           FNSLRLPDGSVANMSVPIVLAIDDAQKH IGDSTSVALFD+ NNPIA++K+IEIYKHPKE
Sbjct: 121 FNSLRLPDGSVANMSVPIVLAIDDAQKHHIGDSTSVALFDANNNPIAIMKDIEIYKHPKE 180

Query: 181 ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT 240
           ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT
Sbjct: 181 ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT 240

Query: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWR 300
           RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNP+LLLHPLGGYTKADDVPL WR
Sbjct: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLHPLGGYTKADDVPLGWR 300

Query: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 361 HPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420
           HPT+KRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG
Sbjct: 361 HPTKKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420

Query: 421 TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPEPEAVLA 468
           TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRL EPEAVLA
Sbjct: 421 TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLAEPEAVLA 469

BLAST of CSPI03G12690 vs. TAIR 10
Match: AT3G22890.1 (ATP sulfurylase 1 )

HSP 1 Score: 774.6 bits (1999), Expect = 4.4e-224
Identity = 376/461 (81.56%), Postives = 406/461 (88.07%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGKL 60
           MASMA   + TP     + ++S  +   P       SKS +  +   + AGLI PDGGKL
Sbjct: 1   MASMAAVLSKTPFLSQPLTKSS-PNSDLPFAAVSFPSKSLRRRVG-SIRAGLIAPDGGKL 60

Query: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLHFN 120
           VEL+VEEP R  KK EA  LPR+EL++ID+QW+HVLSEGWASPL GFMRESEFLQTLHFN
Sbjct: 61  VELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFN 120

Query: 121 SLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKEER 180
           SLRL DGSV NMSVPIVLAIDD QK  IG+ST VALF+S  NP+A+L +IEIYKHPKEER
Sbjct: 121 SLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEER 180

Query: 181 IARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFTRR 240
           IARTWGTTAPGLPYVD+AITNAGNWLIGGDLEV+EP+KY+DGLDRFR SPAELR+E  +R
Sbjct: 181 IARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKELEKR 240

Query: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWRMK 300
           NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGG+TKADDVPL WRMK
Sbjct: 241 NADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDWRMK 300

Query: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360
           QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP
Sbjct: 301 QHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP 360

Query: 361 TEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISGTK 420
            EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSR QDFLFISGTK
Sbjct: 361 VEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTK 420

Query: 421 MRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPE 462
           MRTLAKN ENPP+GFMCPGGWKVLV+YY+SL PA +GRLPE
Sbjct: 421 MRTLAKNNENPPDGFMCPGGWKVLVDYYESLTPAGNGRLPE 459

BLAST of CSPI03G12690 vs. TAIR 10
Match: AT4G14680.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 751.1 bits (1938), Expect = 5.2e-217
Identity = 371/463 (80.13%), Postives = 404/463 (87.26%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPIKVSIA-TSKSKKTHLR-LRVSAGLIEPDGG 60
           MASM+T F   P+ F S Q  + +H    +  SI+  S SK   LR + V AGLIEPDGG
Sbjct: 1   MASMSTVFPK-PTSFIS-QPLTKSHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGG 60

Query: 61  KLVELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTLH 120
           KLV+L+V EP R  KK EA  LPR+ L++ID+QW+HVLSEGWASPL GFMRESEFLQTLH
Sbjct: 61  KLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGWASPLRGFMRESEFLQTLH 120

Query: 121 FNSLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPKE 180
           FN L L DGSV NMSVPIVLAIDD QK  IG+S  V+L DS +NPIA+L +IEIYKHPKE
Sbjct: 121 FNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILNDIEIYKHPKE 180

Query: 181 ERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEFT 240
           ERIARTWGTTAPGLPYV++AITNAG+WLIGGDLEV+EP+KY+DGLDRFR SP ELR+E  
Sbjct: 181 ERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLSPFELRKELE 240

Query: 241 RRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSWR 300
           +R ADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGG+TKADDVPLSWR
Sbjct: 241 KRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLSWR 300

Query: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360
           MKQHEKVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGMG
Sbjct: 301 MKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGMG 360

Query: 361 HPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420
           HP EKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG
Sbjct: 361 HPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFISG 420

Query: 421 TKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSLVPASSGRLPE 462
           TKMR LAKN+ENPP+GFMCPGGWKVLV+YYDSL    + +LPE
Sbjct: 421 TKMRALAKNRENPPDGFMCPGGWKVLVDYYDSLTLTGNTKLPE 461

BLAST of CSPI03G12690 vs. TAIR 10
Match: AT5G43780.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 726.9 bits (1875), Expect = 1.1e-209
Identity = 359/468 (76.71%), Postives = 396/468 (84.62%), Query Frame = 0

Query: 1   MASMATRFTNTPSPFHSIQRTSYTHLG--APIKVSIATSKSKKTHLRLRVSAGLIEPDGG 60
           MAS A     + SPF S       H    AP  +S+ +   + +   L V +GLIEPDGG
Sbjct: 1   MASSAAAIV-SGSPFRSSPLIHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPDGG 60

Query: 61  KLVELLVEEPLRGLKKKEALSLP-RIELSSIDIQWVHVLSEGWASPLTGFMRESEFLQTL 120
           KL+ L+VEE  R + K EA ++P RI+L+ +D++WVHVLSEGWASPL GFMR+SEFLQTL
Sbjct: 61  KLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLKGFMRQSEFLQTL 120

Query: 121 HFNSLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPIAVLKNIEIYKHPK 180
           HFNS RL DGSV NMSVPIVLAIDD QK  IGDS  V L DS  NPIA+L +IEIYKHPK
Sbjct: 121 HFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDIEIYKHPK 180

Query: 181 EERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLDRFRQSPAELREEF 240
           EERIARTWGTTA GLPY ++AIT AGNWLIGGDL+V+EPIKY+DGLDRFR SP++LREEF
Sbjct: 181 EERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSPSQLREEF 240

Query: 241 TRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKADDVPLSW 300
            RR ADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNP+LLL+PLGG+TKADDVPLSW
Sbjct: 241 IRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLGGFTKADDVPLSW 300

Query: 301 RMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360
           RM+QHEKVLEDGVLDPETTVVSIFPSPM YAGPTEVQWHAKARINAGANFYIVGRDPAGM
Sbjct: 301 RMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYIVGRDPAGM 360

Query: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRAQDFLFIS 420
           GHPTEKRDLYDADHGKKVLSMAPGLERLNILPF+VAAYDKTQGKMAFFDPSR+QDFLFIS
Sbjct: 361 GHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFFDPSRSQDFLFIS 420

Query: 421 GTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSL-VPASSGRLPEPEA 465
           GTKMR LAK KENPP+GFMCP GWKVLV+YYDSL     +GR+ E  A
Sbjct: 421 GTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAETGNGRVSEAVA 467

BLAST of CSPI03G12690 vs. TAIR 10
Match: AT1G19920.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 659.8 bits (1701), Expect = 1.6e-189
Identity = 306/407 (75.18%), Postives = 356/407 (87.47%), Query Frame = 0

Query: 45  RLRVSAGLIEPDGGKLVELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEGWASPL 104
           ++ V + LI+PDGG+LVEL+V E   G+KK E+ ++P+++L+ ID++WVHV+SEGWASPL
Sbjct: 57  KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPL 116

Query: 105 TGFMRESEFLQTLHFNSLRLPDGSVANMSVPIVLAIDDAQKHSIGDSTSVALFDSKNNPI 164
            GFMRE E+LQ+LHFNSLRL +G+  NMS+PIVLAIDD  K  IG S +VAL   + + I
Sbjct: 117 KGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDII 176

Query: 165 AVLKNIEIYKHPKEERIARTWGTTAPGLPYVDQAITNAGNWLIGGDLEVIEPIKYHDGLD 224
             L+++EIYKH KEERIARTWGTT+PGLPYV++ IT +GNWLIGGDLEV EPIKY+DGLD
Sbjct: 177 GSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLD 236

Query: 225 RFRQSPAELREEFTRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPL 284
            +R SP +LREEF  R ADAVFAFQLRNPVHNGHALLM DTR+RLLEMGYKNP+LLLHPL
Sbjct: 237 HYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPL 296

Query: 285 GGYTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAG 344
           GG+TKADDVPL  RM+QH KVLEDGVLDP+TT+VSIFPSPMHYAGPTEVQWHAKARINAG
Sbjct: 297 GGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAG 356

Query: 345 ANFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAF 404
           ANFYIVGRDPAGMGHPTEKRDLYD DHGK+VLSMAPGLE+LNILPFRVAAYD  + KMAF
Sbjct: 357 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAF 416

Query: 405 FDPSRAQDFLFISGTKMRTLAKNKENPPEGFMCPGGWKVLVEYYDSL 452
           FDPSRA++FLFISGTKMRT A+  ENPP+GFMCP GW VLV+YY+SL
Sbjct: 417 FDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESL 463

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIK96.2e-22381.56ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV... [more]
O233247.4e-21680.13ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV... [more]
Q9S7D81.5e-20876.71ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV... [more]
Q438702.2e-18875.18ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1[more]
Q271281.7e-14061.76Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase OS=Urechis caupo OX=... [more]
Match NameE-valueIdentityDescription
A0A0A0L5D05.6e-27599.57Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G144230 PE=4 SV=1[more]
A0A1S3AWG31.8e-27399.14Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103483665 PE=4 SV=1[more]
A0A5A7U0F45.2e-27398.93Sulfate adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A6J1FPI43.3e-25993.82Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447315 PE=4 S... [more]
A0A6J1C9161.2e-25693.51Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111009330 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_004134113.11.2e-27499.57ATP sulfurylase 1, chloroplastic [Cucumis sativus] >KGN56933.1 hypothetical prot... [more]
XP_008438607.13.7e-27399.14PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis melo][more]
KAA0049323.11.1e-27298.93ATP sulfurylase 1 [Cucumis melo var. makuwa] >TYK17235.1 ATP sulfurylase 1 [Cucu... [more]
XP_038895326.12.5e-26997.43ATP sulfurylase 1, chloroplastic-like [Benincasa hispida][more]
XP_023539233.16.6e-26294.46ATP sulfurylase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G22890.14.4e-22481.56ATP sulfurylase 1 [more]
AT4G14680.15.2e-21780.13Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT5G43780.11.1e-20976.71Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT1G19920.11.6e-18975.18Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025980ATP-sulfurylase PUA-like domainPFAMPF14306PUA_2coord: 53..215
e-value: 1.1E-45
score: 155.2
IPR002650Sulphate adenylyltransferaseTIGRFAMTIGR00339TIGR00339coord: 55..446
e-value: 4.4E-139
score: 461.5
IPR002650Sulphate adenylyltransferaseCDDcd00517ATPScoord: 80..447
e-value: 1.82148E-167
score: 473.658
NoneNo IPR availableGENE3D3.10.400.10Sulfate adenylyltransferasecoord: 51..219
e-value: 9.5E-59
score: 199.7
NoneNo IPR availablePANTHERPTHR11055:SF41ATP SULFURYLASE 1, CHLOROPLASTICcoord: 1..462
NoneNo IPR availablePANTHERPTHR11055BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASEcoord: 1..462
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 225..448
IPR024951Sulphate adenylyltransferase catalytic domainPFAMPF01747ATP-sulfurylasecoord: 225..447
e-value: 7.7E-67
score: 224.9
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 220..454
e-value: 6.1E-96
score: 322.2
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 53..221

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G12690.1CSPI03G12690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0000103 sulfate assimilation
molecular_function GO:0004020 adenylylsulfate kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004781 sulfate adenylyltransferase (ATP) activity