CSPI03G12600 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G12600
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationChr3: 9597250 .. 9598750 (-)
RNA-Seq ExpressionCSPI03G12600
SyntenyCSPI03G12600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCAACGCAAATTCCAGTGCCCGCCGCCGCTATGGAGACGGGGCTCCGGCCCACTGCCACTGCCACTCCCACTCCCACTCCCACCCCCGCTACTACTCTTCTCCGGAAGTACCAATTAGGCCGCCTTCTCGGTCGTGGAAGTTTCGCCAAAGTCTATCAAGCAGTTTCCTTAGCCGATAACTCCACCGTCGCCATCAAAATCATCGACAAATCCAAAACCATCGGCGCTGTCATGGAGCGTTGCATAGTCCGGGAGGTTGCCGCCATGCGCCGCCTCGATCACCATCCAAACATACTCAAAATCCACGAAGTCATGGCGACAAAGTCCAAAATATACCTGGTGGTTGAATTCGCTGGGGGTGGCGAACTCTTGGCTAAAATCTCCCGCCGTGGGCGATTCACCGAAACCGTCGCGCGCCGGTATTTCCAACAACTCGTCTCGGCCCTTAAGTTCTGCCACGATAACGGCGTCGTTCACCGTGACGTGAAGCCGCAGAATCTTTTGCTGGACGAACAGGGCAATCTGAAAGTCTCTGATTTCGGTCTCTCTGCTTTACCTGAACAACTCAGAGACGGATTACTCCACACGTCTTGTGGAACTCCAGCGTACTCAGCGCCAGAAGTCATGACCTGTCGCAGTGGTAGCGGATACGACGGCGGAAAGGCTGACGCCTGGTCATGTGGAGTAATTTTATTCGTAATGCTCTCTGGGTTTCTCCCTTTCGACGACAGTAATCTAGCGGCGATGTACAGAAAAATCCACAGGCGAGAATTTCAAATCCCTAATTGGGTAACGAAACCTGTCCGATTCCTAATATATCACCTTCTCGATCCGAATCCCAAAACGAGGATGAGCATTGAGGCGTTAATGCAAAACCCCTGGTTCAAGAAATCGCTGCACTTGAAACAGGGCAATGAGAACCTACTGCAATCGATGAGGGATTATCGAATGGGAAAAGAGAGGGGAATTGGGGAAATGAATGCGTTCGATATAATTTCGATGTCTTCGGGGTTGGATTTATCGGGGCTGTTTGAAACGACGGAGAGGAGGGTGGAGAGGAGGTATGCGTCGAGAGCGACGGTGATGGAGGTGGAGAAGAGGGTCAGGGAGATGGGCGTAGAGTTGGAGTATGAAGTGGAGGTGGGGGGAAAGGGAGGGTCGATCGGAATGGGGAAGAGGGGAGTGGTGGTGGTGGTGGAAGTGGTGGAGGTGGCAGTGGCATTAACGATGGTGGAGGTGAGGGTGGTGAAAGGAGGGGAGGAGTTTGAAGAACACCATTGGAGGTGTTTTGAAGGCAAACTTCAAGGATTTGCAGTTCCATGGCAGAGGAAATGTAATTTGTAAAGCTCTTTTTGTTTGTTTGTGTTGGGGAAAGGGAATAGAGTTATAGGGATTTGGAACTTCCAATTTTCGTGTAGTGCTAAACTACAATCGTAAACTTCAATTTAAATTTTGGTTCTGTAAAGTTCTTTTTGTTTTTACATATATGAATGTTTCGA

mRNA sequence

TTCCAACGCAAATTCCAGTGCCCGCCGCCGCTATGGAGACGGGGCTCCGGCCCACTGCCACTGCCACTCCCACTCCCACTCCCACCCCCGCTACTACTCTTCTCCGGAAGTACCAATTAGGCCGCCTTCTCGGTCGTGGAAGTTTCGCCAAAGTCTATCAAGCAGTTTCCTTAGCCGATAACTCCACCGTCGCCATCAAAATCATCGACAAATCCAAAACCATCGGCGCTGTCATGGAGCGTTGCATAGTCCGGGAGGTTGCCGCCATGCGCCGCCTCGATCACCATCCAAACATACTCAAAATCCACGAAGTCATGGCGACAAAGTCCAAAATATACCTGGTGGTTGAATTCGCTGGGGGTGGCGAACTCTTGGCTAAAATCTCCCGCCGTGGGCGATTCACCGAAACCGTCGCGCGCCGGTATTTCCAACAACTCGTCTCGGCCCTTAAGTTCTGCCACGATAACGGCGTCGTTCACCGTGACGTGAAGCCGCAGAATCTTTTGCTGGACGAACAGGGCAATCTGAAAGTCTCTGATTTCGGTCTCTCTGCTTTACCTGAACAACTCAGAGACGGATTACTCCACACGTCTTGTGGAACTCCAGCGTACTCAGCGCCAGAAGTCATGACCTGTCGCAGTGGTAGCGGATACGACGGCGGAAAGGCTGACGCCTGGTCATGTGGAGTAATTTTATTCGTAATGCTCTCTGGGTTTCTCCCTTTCGACGACAGTAATCTAGCGGCGATGTACAGAAAAATCCACAGGCGAGAATTTCAAATCCCTAATTGGGTAACGAAACCTGTCCGATTCCTAATATATCACCTTCTCGATCCGAATCCCAAAACGAGGATGAGCATTGAGGCGTTAATGCAAAACCCCTGGTTCAAGAAATCGCTGCACTTGAAACAGGGCAATGAGAACCTACTGCAATCGATGAGGGATTATCGAATGGGAAAAGAGAGGGGAATTGGGGAAATGAATGCGTTCGATATAATTTCGATGTCTTCGGGGTTGGATTTATCGGGGCTGTTTGAAACGACGGAGAGGAGGGTGGAGAGGAGGTATGCGTCGAGAGCGACGGTGATGGAGGTGGAGAAGAGGGTCAGGGAGATGGGCGTAGAGTTGGAGTATGAAGTGGAGGTGGGGGGAAAGGGAGGGTCGATCGGAATGGGGAAGAGGGGAGTGGTGGTGGTGGTGGAAGTGGTGGAGGTGGCAGTGGCATTAACGATGGTGGAGGTGAGGGTGGTGAAAGGAGGGGAGGAGTTTGAAGAACACCATTGGAGGTGTTTTGAAGGCAAACTTCAAGGATTTGCAGTTCCATGGCAGAGGAAATGTAATTTGTAAAGCTCTTTTTGTTTGTTTGTGTTGGGGAAAGGGAATAGAGTTATAGGGATTTGGAACTTCCAATTTTCGTGTAGTGCTAAACTACAATCGTAAACTTCAATTTAAATTTTGGTTCTGTAAAGTTCTTTTTGTTTTTACATATATGAATGTTTCGA

Coding sequence (CDS)

ATGGAGACGGGGCTCCGGCCCACTGCCACTGCCACTCCCACTCCCACTCCCACCCCCGCTACTACTCTTCTCCGGAAGTACCAATTAGGCCGCCTTCTCGGTCGTGGAAGTTTCGCCAAAGTCTATCAAGCAGTTTCCTTAGCCGATAACTCCACCGTCGCCATCAAAATCATCGACAAATCCAAAACCATCGGCGCTGTCATGGAGCGTTGCATAGTCCGGGAGGTTGCCGCCATGCGCCGCCTCGATCACCATCCAAACATACTCAAAATCCACGAAGTCATGGCGACAAAGTCCAAAATATACCTGGTGGTTGAATTCGCTGGGGGTGGCGAACTCTTGGCTAAAATCTCCCGCCGTGGGCGATTCACCGAAACCGTCGCGCGCCGGTATTTCCAACAACTCGTCTCGGCCCTTAAGTTCTGCCACGATAACGGCGTCGTTCACCGTGACGTGAAGCCGCAGAATCTTTTGCTGGACGAACAGGGCAATCTGAAAGTCTCTGATTTCGGTCTCTCTGCTTTACCTGAACAACTCAGAGACGGATTACTCCACACGTCTTGTGGAACTCCAGCGTACTCAGCGCCAGAAGTCATGACCTGTCGCAGTGGTAGCGGATACGACGGCGGAAAGGCTGACGCCTGGTCATGTGGAGTAATTTTATTCGTAATGCTCTCTGGGTTTCTCCCTTTCGACGACAGTAATCTAGCGGCGATGTACAGAAAAATCCACAGGCGAGAATTTCAAATCCCTAATTGGGTAACGAAACCTGTCCGATTCCTAATATATCACCTTCTCGATCCGAATCCCAAAACGAGGATGAGCATTGAGGCGTTAATGCAAAACCCCTGGTTCAAGAAATCGCTGCACTTGAAACAGGGCAATGAGAACCTACTGCAATCGATGAGGGATTATCGAATGGGAAAAGAGAGGGGAATTGGGGAAATGAATGCGTTCGATATAATTTCGATGTCTTCGGGGTTGGATTTATCGGGGCTGTTTGAAACGACGGAGAGGAGGGTGGAGAGGAGGTATGCGTCGAGAGCGACGGTGATGGAGGTGGAGAAGAGGGTCAGGGAGATGGGCGTAGAGTTGGAGTATGAAGTGGAGGTGGGGGGAAAGGGAGGGTCGATCGGAATGGGGAAGAGGGGAGTGGTGGTGGTGGTGGAAGTGGTGGAGGTGGCAGTGGCATTAACGATGGTGGAGGTGAGGGTGGTGAAAGGAGGGGAGGAGTTTGAAGAACACCATTGGAGGTGTTTTGAAGGCAAACTTCAAGGATTTGCAGTTCCATGGCAGAGGAAATGTAATTTGTAA

Protein sequence

METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRRGRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLRDGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQSMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVREMGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEHHWRCFEGKLQGFAVPWQRKCNL*
Homology
BLAST of CSPI03G12600 vs. ExPASy Swiss-Prot
Match: Q9XIW0 (CBL-interacting serine/threonine-protein kinase 7 OS=Arabidopsis thaliana OX=3702 GN=CIPK7 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.2e-128
Identity = 240/423 (56.74%), Postives = 314/423 (74.23%), Query Frame = 0

Query: 14  TPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIGAVMERCIV 73
           +P  TPA  LL KY+LGR LG GSFAKV+ A S+  +  VA+KII+K KTI + ME  I+
Sbjct: 12  SPATTPAKILLGKYELGRRLGSGSFAKVHLARSIESDELVAVKIIEKKKTIESGMEPRII 71

Query: 74  REVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRRGRFTETVARRYFQ 133
           RE+ AMRRL HHPNILKIHEVMATKSKIYLV+E A GGEL +K+ RRGR  E+ ARRYFQ
Sbjct: 72  REIDAMRRLRHHPNILKIHEVMATKSKIYLVMELASGGELFSKVLRRGRLPESTARRYFQ 131

Query: 134 QLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLRDGLLHTSCGTPAY 193
           QL SAL+F H +GV HRDVKPQNLLLDEQGNLKVSDFGLSALPE L++GLLHT+CGTPAY
Sbjct: 132 QLASALRFSHQDGVAHRDVKPQNLLLDEQGNLKVSDFGLSALPEHLQNGLLHTACGTPAY 191

Query: 194 SAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPNW 253
           +APEV++ R   GYDG KADAWSCGVILFV+L G +PFDDSN+AAMYRKIHRR+++ P+W
Sbjct: 192 TAPEVISRR---GYDGAKADAWSCGVILFVLLVGDVPFDDSNIAAMYRKIHRRDYRFPSW 251

Query: 254 VTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQSMRDYRMGKERGI 313
           ++K  + +IY +LDPNP TRMSIE +M+  WFKKSL   + + N+     D  +  +  +
Sbjct: 252 ISKQAKSIIYQMLDPNPVTRMSIETVMKTNWFKKSLETSEFHRNVF----DSEVEMKSSV 311

Query: 314 GEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVREMGVELEYEV---- 373
             + AFD+IS+SSGLDLSGLFE  +++ ERR+ ++ + +EVE++ + +G +L Y V    
Sbjct: 312 NSITAFDLISLSSGLDLSGLFEAKKKK-ERRFTAKVSGVEVEEKAKMIGEKLGYVVKKKM 371

Query: 374 -EVGGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEHHWRCFEGKLQGFA 432
            +  G+   +G+G+   V+VVE VE+ V + +VEV+VV+G E  ++  W     +L+   
Sbjct: 372 MKKEGEVKVVGLGRGRTVIVVEAVELTVDVVVVEVKVVEGEE--DDSRWSDLITELEDIV 424

BLAST of CSPI03G12600 vs. ExPASy Swiss-Prot
Match: Q9SUL7 (CBL-interacting serine/threonine-protein kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CIPK4 PE=1 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 7.3e-125
Identity = 237/422 (56.16%), Postives = 306/422 (72.51%), Query Frame = 0

Query: 14  TPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIGAVMERCIV 73
           +P     T LL KY+LGR LG GSFAKV+ A S++    VAIKIIDK KTI + ME  I+
Sbjct: 8   SPEKITGTVLLGKYELGRRLGSGSFAKVHVARSISTGELVAIKIIDKQKTIDSGMEPRII 67

Query: 74  REVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRRGRFTETVARRYFQ 133
           RE+ AMRRL +HPN+LKIHEVMATKSKIYLVVE+A GGEL  K+ R GR  E+ ARRYFQ
Sbjct: 68  REIEAMRRLHNHPNVLKIHEVMATKSKIYLVVEYAAGGELFTKLIRFGRLNESAARRYFQ 127

Query: 134 QLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPE-QLRDGLLHTSCGTPA 193
           QL SAL FCH +G+ HRDVKPQNLLLD+QGNLKVSDFGLSALPE +  +GLLHT+CGTPA
Sbjct: 128 QLASALSFCHRDGIAHRDVKPQNLLLDKQGNLKVSDFGLSALPEHRSNNGLLHTACGTPA 187

Query: 194 YSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPN 253
           Y+APEV+  R   GYDG KADAWSCGV LFV+L+G++PFDD+N+ AMYRKIH+R+++ P+
Sbjct: 188 YTAPEVIAQR---GYDGAKADAWSCGVFLFVLLAGYVPFDDANIVAMYRKIHKRDYRFPS 247

Query: 254 WVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQSMRDYRMGKERG 313
           W++KP R +IY LLDPNP+TRMSIEA+M   WF+KSL + +   ++ +  R +   + + 
Sbjct: 248 WISKPARSIIYKLLDPNPETRMSIEAVMGTVWFQKSLEISEFQSSVFELDR-FLEKEAKS 307

Query: 314 IGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVREMGVELEYEVEVG 373
              + AFD+IS+SSGLDLSGLFE  +R+ E+R+ +R +   V ++   +G +L + VE  
Sbjct: 308 SNAITAFDLISLSSGLDLSGLFERRKRK-EKRFTARVSAERVVEKAGMIGEKLGFRVEKK 367

Query: 374 GKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEH---HWRCFEGKLQGFAV 432
            +   +G+GK    VVVEVVE A  L + +V+VV  GEE EE    HW     +L+   +
Sbjct: 368 EETKVVGLGKGRTAVVVEVVEFAEGLVVADVKVVVEGEEEEEEVESHWSELIVELEEIVL 424

BLAST of CSPI03G12600 vs. ExPASy Swiss-Prot
Match: Q75GK4 (CBL-interacting protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK7 PE=2 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.8e-100
Identity = 214/411 (52.07%), Postives = 289/411 (70.32%), Query Frame = 0

Query: 11  ATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNS-TVAIKIIDKS--KTIGAV 70
           AT +        LL KY+LGRLLGRG+FAKVY A SLA  +  VA+K++DK      GA 
Sbjct: 3   ATKSKAAKKGAPLLGKYELGRLLGRGTFAKVYHARSLAPGADPVAVKVLDKPDLAAAGAG 62

Query: 71  MERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISR--RGRFTE 130
           M   ++REVAAMRRL  HPN+L++HEV+AT+SK+YLV+E A GG+LL++++     R  E
Sbjct: 63  MATRVLREVAAMRRL-RHPNVLRLHEVLATRSKVYLVMELAPGGDLLSRLASLPSRRLPE 122

Query: 131 TVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR-DGLL 190
             A+R F QLVSAL +CH  GV HRDVKPQN+LLD  GNLKVSDFGL+ALP+ LR DG L
Sbjct: 123 HAAQRVFLQLVSALIYCHARGVSHRDVKPQNVLLDAHGNLKVSDFGLAALPDSLRDDGRL 182

Query: 191 HTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIH 250
           HT+CGTPA++APEV+  ++   YDG KADAWSCGVILFV+L+G LPFDDSN+A M RK H
Sbjct: 183 HTACGTPAFAAPEVLRRKA---YDGAKADAWSCGVILFVLLAGHLPFDDSNIADMCRKAH 242

Query: 251 RREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQSMRD 310
           RRE+ +P WV++P R L+  LLDPNP TR+++  L  +PWFK+SL L     +LL    +
Sbjct: 243 RREYALPRWVSQPARRLVSRLLDPNPATRLAVAELATHPWFKRSLSLDSQLGSLLGGQPE 302

Query: 311 YRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVREMGVE 370
             +  +     +NAFDIISMS GLDLSGLF  ++RR E+R+ + A+     +R+ + G +
Sbjct: 303 RELAFQ-APPPLNAFDIISMSPGLDLSGLFGESKRRREKRFVTTASPERTVERLGQAGAK 362

Query: 371 LEYEVEVGGKGGS---IGMGKRGVVVV-VEVVEVAVALTMVEVRVVKGGEE 412
           L Y + VG KG     +G G  G+V + +E+ EV+ ++ +VE+R ++GG++
Sbjct: 363 LGYFM-VGKKGVERLPLG-GLSGLVAMSMEMSEVSPSMMLVELR-LEGGDD 405

BLAST of CSPI03G12600 vs. ExPASy Swiss-Prot
Match: Q2QMI0 (CBL-interacting protein kinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK4 PE=2 SV=2)

HSP 1 Score: 366.7 bits (940), Expect = 3.7e-100
Identity = 212/424 (50.00%), Postives = 294/424 (69.34%), Query Frame = 0

Query: 23  LLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIG-AVMERCIVREVAAMRR 82
           LL KY+LGR+LGRG+FAKVY A ++A    VA+K+IDK++ +G A M   ++REVAAMRR
Sbjct: 21  LLGKYELGRMLGRGTFAKVYLARAVAGGEAVAVKVIDKAEVMGTAGMAPRVLREVAAMRR 80

Query: 83  LDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISR--RGRFTETVARRYFQQLVSAL 142
           L  HP++L++HEV+AT+++IYLV+E A GG+LL++++   R R  E+ ARR F QLV AL
Sbjct: 81  L-RHPHVLRLHEVLATRARIYLVMELATGGDLLSRLAALPRRRLPESAARRVFVQLVDAL 140

Query: 143 KFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR-DGLLHTSCGTPAYSAPEV 202
            +CH  GV HRDVKPQN+LLD  GNLKVSDFGL+ALP+ LR DG LHT+CGTPAY+APEV
Sbjct: 141 SYCHARGVAHRDVKPQNVLLDGDGNLKVSDFGLAALPDTLRDDGRLHTACGTPAYAAPEV 200

Query: 203 MTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPNWVTKPV 262
           +  R+   YDG KADAWSCGVILFV+L+G LPFDDSN+A M RK HRRE+++P WV++P 
Sbjct: 201 LRRRA---YDGAKADAWSCGVILFVLLAGHLPFDDSNIADMCRKAHRREYELPRWVSQPA 260

Query: 263 RFLIYHLLDPNPKTRMSIEAL-MQNPWFKKSLHLKQGNENLLQSMRDYRMGKERG-IGEM 322
           R L+  LLDPNP TR+++E+L   +PWFK+SL +    + LL    +  +  +      +
Sbjct: 261 RRLVSRLLDPNPDTRVAVESLAAHHPWFKRSLSVDSQLDGLLNGEPERAVAFQAAPPPPL 320

Query: 323 NAFDIISMSSGLDLSGLFETTERRV-ERRYASRATVMEVEKRVREMGVELEYEVEVGGKG 382
           NAFDIISMS GLDLSGLF   ++ + E+R+ + A+  +  +++   G +L Y V VG KG
Sbjct: 321 NAFDIISMSPGLDLSGLFGEHDKSLREKRFTTTASPEKTLEQLGLAGGKLGYVVVVGKKG 380

Query: 383 GSI-----GMGKRGVVVV-VEVVEVAVALTMVEVRVVKGGEEFEEHHWRCFEGKLQGFAV 434
                   G    G+  + VE+ EVA  L +VE+R+     + +       +G+++GF  
Sbjct: 381 VECLPLAGGRLSSGIAAMSVEMSEVAPPLLLVELRLEVAAGDVDGG-----DGEVKGFG- 433

BLAST of CSPI03G12600 vs. ExPASy Swiss-Prot
Match: P92937 (CBL-interacting serine/threonine-protein kinase 15 OS=Arabidopsis thaliana OX=3702 GN=CIPK15 PE=1 SV=2)

HSP 1 Score: 351.3 bits (900), Expect = 1.6e-95
Identity = 197/402 (49.00%), Postives = 266/402 (66.17%), Query Frame = 0

Query: 21  TTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIGAVMERCIVREVAAMR 80
           + L+ +Y++G+ LG+G+FAKVY A  L    +VAIK+IDK + +   M   I RE++AMR
Sbjct: 6   SVLMLRYEVGKFLGQGTFAKVYHARHLKTGDSVAIKVIDKERILKVGMTEQIKREISAMR 65

Query: 81  RLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRRGRFTETVARRYFQQLVSALK 140
            L  HPNI+++HEVMATKSKIY V+E   GGEL  K+S  G+  E VAR+YFQQLV A+ 
Sbjct: 66  LL-RHPNIVELHEVMATKSKIYFVMEHVKGGELFNKVS-TGKLREDVARKYFQQLVRAVD 125

Query: 141 FCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR-DGLLHTSCGTPAYSAPEVM 200
           FCH  GV HRD+KP+NLLLDE GNLK+SDFGLSAL +  R DGLLHT+CGTPAY APEV+
Sbjct: 126 FCHSRGVCHRDLKPENLLLDEHGNLKISDFGLSALSDSRRQDGLLHTTCGTPAYVAPEVI 185

Query: 201 TCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPNWVTKPVR 260
              S +GYDG KAD WSCGVILFV+L+G+LPF DSNL  +Y+KI + E + PNW+    +
Sbjct: 186 ---SRNGYDGFKADVWSCGVILFVLLAGYLPFRDSNLMELYKKIGKAEVKFPNWLAPGAK 245

Query: 261 FLIYHLLDPNPKTRMSIEALMQNPWFKKSL--HLKQGNEN------LLQSMRDYRMGKER 320
            L+  +LDPNP TR+S E +M++ WF+K L   +K+  E         +        K+R
Sbjct: 246 RLLKRILDPNPNTRVSTEKIMKSSWFRKGLQEEVKESVEEETEVDAEAEGNASAEKEKKR 305

Query: 321 GIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVREMGVELEYEVEV 380
            I  +NAF+IIS+S+G DLSGLFE  E + E R+ S     E+ +++ E+G +L+ +V  
Sbjct: 306 CI-NLNAFEIISLSTGFDLSGLFEKGEEKEEMRFTSNREASEITEKLVEIGKDLKMKVRK 365

Query: 381 GGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFE 414
                 + M     VV  EV E+A +  MV ++   GG+  E
Sbjct: 366 KEHEWRVKMSAEATVVEAEVFEIAPSYHMVVLK-KSGGDTAE 400

BLAST of CSPI03G12600 vs. ExPASy TrEMBL
Match: A0A0A0L5B5 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_3G144130 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.5e-242
Identity = 430/437 (98.40%), Postives = 431/437 (98.63%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           METGLRPTA    TPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK
Sbjct: 29  METGLRPTA----TPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 88

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR
Sbjct: 89  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 148

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR
Sbjct: 149 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 208

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY
Sbjct: 209 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 268

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           RKIHRREFQIPNWV+KPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQ NENLLQ
Sbjct: 269 RKIHRREFQIPNWVSKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQANENLLQ 328

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERR ERRYASRATVMEVEKRVRE
Sbjct: 329 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRAERRYASRATVMEVEKRVRE 388

Query: 361 MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEHHWRCF 420
           MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEHHWRCF
Sbjct: 389 MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEHHWRCF 448

Query: 421 EGKLQGFAVPWQRKCNL 438
           EGKLQGFAVPWQRKCNL
Sbjct: 449 EGKLQGFAVPWQRKCNL 461

BLAST of CSPI03G12600 vs. ExPASy TrEMBL
Match: A0A5D3D0F1 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00960 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 7.6e-226
Identity = 406/440 (92.27%), Postives = 416/440 (94.55%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           METGLRPT +A P         LL KYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK
Sbjct: 1   METGLRPTPSAAP---------LLGKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR
Sbjct: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTETVARRYFQQLVSALKFCH+NGVVHRDVKPQNLLLDEQGNLK+SDFGLSALPEQLR
Sbjct: 121 GRFTETVARRYFQQLVSALKFCHENGVVHRDVKPQNLLLDEQGNLKISDFGLSALPEQLR 180

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           DGLLHTSCGTPAYSAPEVMTCRSG GYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY
Sbjct: 181 DGLLHTSCGTPAYSAPEVMTCRSGGGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           RKI RREFQIP+WV+KPVRFLIYHLLDPNPKTRMSI+ALMQNPWFKKSL LKQGN NLLQ
Sbjct: 241 RKIRRREFQIPDWVSKPVRFLIYHLLDPNPKTRMSIDALMQNPWFKKSLQLKQGNGNLLQ 300

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SMRDYRMGKERGIGEMNAFDIISMSSGL+LSGLFETTERR ERRY SRATVMEVE+RVRE
Sbjct: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLNLSGLFETTERRAERRYTSRATVMEVEERVRE 360

Query: 361 MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAV---ALTMVEVRVVKGGEEFEEHHW 420
           MG EL YEVEVGGKGG+IGMGKRGVVVVVEVVEVAV   ALTMVEVRVVKGGEEFEE HW
Sbjct: 361 MGGELGYEVEVGGKGGAIGMGKRGVVVVVEVVEVAVAPAALTMVEVRVVKGGEEFEELHW 420

Query: 421 RCFEGKLQGFAVPWQRKCNL 438
           RCFEGKL+GFAVPWQRKCNL
Sbjct: 421 RCFEGKLEGFAVPWQRKCNL 431

BLAST of CSPI03G12600 vs. ExPASy TrEMBL
Match: A0A1S3AXF7 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103483656 PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 8.5e-225
Identity = 404/440 (91.82%), Postives = 414/440 (94.09%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           METGLRPT +A P         LL KYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK
Sbjct: 1   METGLRPTPSAAP---------LLGKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR
Sbjct: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTETVARRYFQQLVSALKFCH+NGVVHRDVKPQNLLLDEQGNLK+SDFGLSALPEQLR
Sbjct: 121 GRFTETVARRYFQQLVSALKFCHENGVVHRDVKPQNLLLDEQGNLKISDFGLSALPEQLR 180

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           DGLLHTSCGTPAYSAPEVMTCRSG GYDGGKADAWSCGVILFVMLSGFLPFDDSNL AMY
Sbjct: 181 DGLLHTSCGTPAYSAPEVMTCRSGGGYDGGKADAWSCGVILFVMLSGFLPFDDSNLGAMY 240

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           RKI RREFQIP+WV+KPVRFLIYHLLDPNPKTRMSI+ALMQNPWFKKSL LKQGN NLLQ
Sbjct: 241 RKIRRREFQIPDWVSKPVRFLIYHLLDPNPKTRMSIDALMQNPWFKKSLQLKQGNGNLLQ 300

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SMRDYRMGKERGIGEMNAFDIISMSSGL+LSGLFETTERR ERRY SRATVMEVE+RVRE
Sbjct: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLNLSGLFETTERRAERRYTSRATVMEVEERVRE 360

Query: 361 MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAV---ALTMVEVRVVKGGEEFEEHHW 420
           MG EL YEVE GGKGG+IGMGKRGVVVVVEVVEVAV   ALTMVEVRVVKGGEEFEE HW
Sbjct: 361 MGGELGYEVEXGGKGGAIGMGKRGVVVVVEVVEVAVAPAALTMVEVRVVKGGEEFEELHW 420

Query: 421 RCFEGKLQGFAVPWQRKCNL 438
           RCFEGKL+GFAVPWQRKCNL
Sbjct: 421 RCFEGKLEGFAVPWQRKCNL 431

BLAST of CSPI03G12600 vs. ExPASy TrEMBL
Match: A0A6J1E941 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111431801 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 6.3e-204
Identity = 368/442 (83.26%), Postives = 397/442 (89.82%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           M+ GLRPT  A P    +  TTLL KY LGRLLGRGSFAKVYQAVSLADNS VA+KIIDK
Sbjct: 1   MKPGLRPTPAAVPR---SAGTTLLGKYGLGRLLGRGSFAKVYQAVSLADNSIVAVKIIDK 60

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKT+GA +ERCIVRE+AAMRRLDHHPNILKIHEVMATKSKIYLVVEFA GGELLAKISRR
Sbjct: 61  SKTVGATLERCIVREIAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAAGGELLAKISRR 120

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTETVARRYFQQLVSALKFCH+NGVVHRDVKPQNLLLDEQGNLK+SDFGLSALPE+L+
Sbjct: 121 GRFTETVARRYFQQLVSALKFCHENGVVHRDVKPQNLLLDEQGNLKISDFGLSALPEKLK 180

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           D LLHT+CGTPAYSAPEVM  R   GYDGGKADAWSCGVILFVMLSGFLPF+D NLAAMY
Sbjct: 181 DELLHTACGTPAYSAPEVMIRRGNRGYDGGKADAWSCGVILFVMLSGFLPFEDRNLAAMY 240

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           +KIHRREFQIP W++KPVRFLIYHLLDPNP+TRMSIEALMQNPWFKKSL  KQ NE+L+Q
Sbjct: 241 KKIHRREFQIPKWISKPVRFLIYHLLDPNPETRMSIEALMQNPWFKKSLARKQSNESLMQ 300

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SM++YR+GKERGIGEMNAFDIISMSSGLDLSGLFETTE+R ERR+ SRATV EVE+RVRE
Sbjct: 301 SMKNYRLGKERGIGEMNAFDIISMSSGLDLSGLFETTEKRAERRFTSRATVREVEERVRE 360

Query: 361 MGVELEYEVEVG--GKGGSIGMGKRGVVVVVEVVEVAVA---LTMVEVRVVKGGEEFEEH 420
           +G  L YEVE     KGG+IGMGK  VVVVVEVVE+AVA   LTMVEVRV+KGGEEFEE 
Sbjct: 361 IGGVLGYEVEAEAVAKGGAIGMGKGRVVVVVEVVEMAVAPVTLTMVEVRVIKGGEEFEEL 420

Query: 421 HWRCFEGKLQGFAVPWQRKCNL 438
           HWRCFE KL+GFAVPWQ KCNL
Sbjct: 421 HWRCFEAKLEGFAVPWQMKCNL 439

BLAST of CSPI03G12600 vs. ExPASy TrEMBL
Match: A0A6J1IS98 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111480085 PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 7.0e-203
Identity = 366/442 (82.81%), Postives = 396/442 (89.59%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           M+ GLRPT  A P    +   TLL KY LGRLLGRGSFAKVYQAVSLADNS VA+KIIDK
Sbjct: 1   MKPGLRPTPAAVPR---SAGATLLGKYGLGRLLGRGSFAKVYQAVSLADNSIVAVKIIDK 60

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKT+GA +ERCIVRE+AAMRRLD HPNILKIHEVMATKSKIYLVVEFA GGELLAKISRR
Sbjct: 61  SKTVGATLERCIVREIAAMRRLDRHPNILKIHEVMATKSKIYLVVEFAAGGELLAKISRR 120

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTETVARRYFQQLVSALKFCH+NGVVHRDVKPQNLLLDEQGNLK+SDFGLSALPE+L+
Sbjct: 121 GRFTETVARRYFQQLVSALKFCHENGVVHRDVKPQNLLLDEQGNLKISDFGLSALPEKLK 180

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           D LLHT+CGTPAYSAPEVM  R   GYDGGKADAWSCGVILFVMLSGFLPF+DSNLAAMY
Sbjct: 181 DELLHTACGTPAYSAPEVMIRRGNRGYDGGKADAWSCGVILFVMLSGFLPFEDSNLAAMY 240

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           +KIHRREF+IP W++KPVRFLIYHLLDPNP+TRMSIEALMQNPWFKKSL  KQ NE+L+Q
Sbjct: 241 KKIHRREFEIPKWISKPVRFLIYHLLDPNPETRMSIEALMQNPWFKKSLARKQSNESLMQ 300

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SM++YR+GKERGIGEMNAFDIISMSSGLDLSGLFETTE+R ERR+ SRATV EVE+RVRE
Sbjct: 301 SMKNYRLGKERGIGEMNAFDIISMSSGLDLSGLFETTEKRAERRFTSRATVREVEERVRE 360

Query: 361 MGVELEYEVEVG--GKGGSIGMGKRGVVVVVEVVEVAVA---LTMVEVRVVKGGEEFEEH 420
           +G  L YEVE     KGG+IGMGK  VVVVVEVVE+AVA   LTMVEVRV+KGGEEFEE 
Sbjct: 361 IGGVLGYEVEAEAVAKGGAIGMGKGRVVVVVEVVEMAVALVTLTMVEVRVIKGGEEFEEF 420

Query: 421 HWRCFEGKLQGFAVPWQRKCNL 438
           HWRCFE KL+GFAVPWQ KCNL
Sbjct: 421 HWRCFEAKLEGFAVPWQMKCNL 439

BLAST of CSPI03G12600 vs. NCBI nr
Match: XP_004134430.2 (CBL-interacting serine/threonine-protein kinase 7 [Cucumis sativus] >KGN56948.2 hypothetical protein Csa_010070 [Cucumis sativus])

HSP 1 Score: 848.2 bits (2190), Expect = 3.2e-242
Identity = 430/437 (98.40%), Postives = 431/437 (98.63%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           METGLRPTA    TPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK
Sbjct: 1   METGLRPTA----TPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR
Sbjct: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR
Sbjct: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY
Sbjct: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           RKIHRREFQIPNWV+KPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQ NENLLQ
Sbjct: 241 RKIHRREFQIPNWVSKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQANENLLQ 300

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERR ERRYASRATVMEVEKRVRE
Sbjct: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRAERRYASRATVMEVEKRVRE 360

Query: 361 MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEHHWRCF 420
           MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEHHWRCF
Sbjct: 361 MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEHHWRCF 420

Query: 421 EGKLQGFAVPWQRKCNL 438
           EGKLQGFAVPWQRKCNL
Sbjct: 421 EGKLQGFAVPWQRKCNL 433

BLAST of CSPI03G12600 vs. NCBI nr
Match: KAA0049313.1 (CBL-interacting serine/threonine-protein kinase 7-like [Cucumis melo var. makuwa] >TYK17245.1 CBL-interacting serine/threonine-protein kinase 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 792.7 bits (2046), Expect = 1.6e-225
Identity = 406/440 (92.27%), Postives = 416/440 (94.55%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           METGLRPT +A P         LL KYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK
Sbjct: 1   METGLRPTPSAAP---------LLGKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR
Sbjct: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTETVARRYFQQLVSALKFCH+NGVVHRDVKPQNLLLDEQGNLK+SDFGLSALPEQLR
Sbjct: 121 GRFTETVARRYFQQLVSALKFCHENGVVHRDVKPQNLLLDEQGNLKISDFGLSALPEQLR 180

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           DGLLHTSCGTPAYSAPEVMTCRSG GYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY
Sbjct: 181 DGLLHTSCGTPAYSAPEVMTCRSGGGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           RKI RREFQIP+WV+KPVRFLIYHLLDPNPKTRMSI+ALMQNPWFKKSL LKQGN NLLQ
Sbjct: 241 RKIRRREFQIPDWVSKPVRFLIYHLLDPNPKTRMSIDALMQNPWFKKSLQLKQGNGNLLQ 300

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SMRDYRMGKERGIGEMNAFDIISMSSGL+LSGLFETTERR ERRY SRATVMEVE+RVRE
Sbjct: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLNLSGLFETTERRAERRYTSRATVMEVEERVRE 360

Query: 361 MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAV---ALTMVEVRVVKGGEEFEEHHW 420
           MG EL YEVEVGGKGG+IGMGKRGVVVVVEVVEVAV   ALTMVEVRVVKGGEEFEE HW
Sbjct: 361 MGGELGYEVEVGGKGGAIGMGKRGVVVVVEVVEVAVAPAALTMVEVRVVKGGEEFEELHW 420

Query: 421 RCFEGKLQGFAVPWQRKCNL 438
           RCFEGKL+GFAVPWQRKCNL
Sbjct: 421 RCFEGKLEGFAVPWQRKCNL 431

BLAST of CSPI03G12600 vs. NCBI nr
Match: XP_008438596.2 (PREDICTED: LOW QUALITY PROTEIN: CBL-interacting serine/threonine-protein kinase 7-like [Cucumis melo])

HSP 1 Score: 789.3 bits (2037), Expect = 1.7e-224
Identity = 404/440 (91.82%), Postives = 414/440 (94.09%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           METGLRPT +A P         LL KYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK
Sbjct: 1   METGLRPTPSAAP---------LLGKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR
Sbjct: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTETVARRYFQQLVSALKFCH+NGVVHRDVKPQNLLLDEQGNLK+SDFGLSALPEQLR
Sbjct: 121 GRFTETVARRYFQQLVSALKFCHENGVVHRDVKPQNLLLDEQGNLKISDFGLSALPEQLR 180

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           DGLLHTSCGTPAYSAPEVMTCRSG GYDGGKADAWSCGVILFVMLSGFLPFDDSNL AMY
Sbjct: 181 DGLLHTSCGTPAYSAPEVMTCRSGGGYDGGKADAWSCGVILFVMLSGFLPFDDSNLGAMY 240

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           RKI RREFQIP+WV+KPVRFLIYHLLDPNPKTRMSI+ALMQNPWFKKSL LKQGN NLLQ
Sbjct: 241 RKIRRREFQIPDWVSKPVRFLIYHLLDPNPKTRMSIDALMQNPWFKKSLQLKQGNGNLLQ 300

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SMRDYRMGKERGIGEMNAFDIISMSSGL+LSGLFETTERR ERRY SRATVMEVE+RVRE
Sbjct: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLNLSGLFETTERRAERRYTSRATVMEVEERVRE 360

Query: 361 MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAV---ALTMVEVRVVKGGEEFEEHHW 420
           MG EL YEVE GGKGG+IGMGKRGVVVVVEVVEVAV   ALTMVEVRVVKGGEEFEE HW
Sbjct: 361 MGGELGYEVEXGGKGGAIGMGKRGVVVVVEVVEVAVAPAALTMVEVRVVKGGEEFEELHW 420

Query: 421 RCFEGKLQGFAVPWQRKCNL 438
           RCFEGKL+GFAVPWQRKCNL
Sbjct: 421 RCFEGKLEGFAVPWQRKCNL 431

BLAST of CSPI03G12600 vs. NCBI nr
Match: XP_038900771.1 (CBL-interacting serine/threonine-protein kinase 7-like [Benincasa hispida])

HSP 1 Score: 753.1 bits (1943), Expect = 1.4e-213
Identity = 380/440 (86.36%), Postives = 403/440 (91.59%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           METGLRPT TA P   P+   TLL KY LGRLLGRGSFAKVYQ VSL DNSTVA+KIIDK
Sbjct: 1   METGLRPTPTAAP---PSTGATLLGKYHLGRLLGRGSFAKVYQVVSLVDNSTVAVKIIDK 60

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKT+GA +ERCIVREVAAMRRL+HHPNILKIHEVMATKSKIYLVVEFA GGEL AKISRR
Sbjct: 61  SKTVGAALERCIVREVAAMRRLNHHPNILKIHEVMATKSKIYLVVEFAAGGELFAKISRR 120

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTE VARRYFQQLVSALKFCH+NGVVHRDVKPQNLLLDEQGNLK+SDFGLSALPEQLR
Sbjct: 121 GRFTEPVARRYFQQLVSALKFCHENGVVHRDVKPQNLLLDEQGNLKISDFGLSALPEQLR 180

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           DGLLHT+CGTPAYSAPEVM  R G GYDGGKADAWSCGVILFVML GFLPFDDSNLAAMY
Sbjct: 181 DGLLHTACGTPAYSAPEVMIRRGGGGYDGGKADAWSCGVILFVMLCGFLPFDDSNLAAMY 240

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           +KIHRREFQIP W++KPVRFLIYHLLDPNPKTRMSIEALMQ+PWFKKSL LKQ NE+LLQ
Sbjct: 241 KKIHRREFQIPIWISKPVRFLIYHLLDPNPKTRMSIEALMQSPWFKKSLQLKQSNESLLQ 300

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SMRDYR+GKERGIGEMNAFDIISMSSGLDLSGLFET E+R ERR+ SR TVMEVE+R++E
Sbjct: 301 SMRDYRLGKERGIGEMNAFDIISMSSGLDLSGLFETAEKRAERRFTSRETVMEVEERMKE 360

Query: 361 MGVELEYEVEVGGKGGSIGMGKRGVVVVVEVVEVAVA---LTMVEVRVVKGGEEFEEHHW 420
           +G EL YEVE G KGG+IGMGK  VVVVVEVVEVAVA   LTMVEVRVV+GGEEFEEHHW
Sbjct: 361 IGGELGYEVEAGAKGGAIGMGKGRVVVVVEVVEVAVAPATLTMVEVRVVRGGEEFEEHHW 420

Query: 421 RCFEGKLQGFAVPWQRKCNL 438
           RCFEGKL+GFAVPWQRKCNL
Sbjct: 421 RCFEGKLEGFAVPWQRKCNL 437

BLAST of CSPI03G12600 vs. NCBI nr
Match: XP_023527173.1 (CBL-interacting serine/threonine-protein kinase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 719.9 bits (1857), Expect = 1.3e-203
Identity = 368/442 (83.26%), Postives = 398/442 (90.05%), Query Frame = 0

Query: 1   METGLRPTATATPTPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDK 60
           M+ GLRPT  A P    +  TTLL KY LGRLLGRGSFAKVYQAVSLADNS VA+KIIDK
Sbjct: 1   MKPGLRPTPAAVPR---SAGTTLLGKYGLGRLLGRGSFAKVYQAVSLADNSIVAVKIIDK 60

Query: 61  SKTIGAVMERCIVREVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRR 120
           SKT+GA +ERCIVRE+AAMRRLDHHPNILKIHEVMATKSKIYLVVEFA GGELLAKISRR
Sbjct: 61  SKTVGATLERCIVREIAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAAGGELLAKISRR 120

Query: 121 GRFTETVARRYFQQLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR 180
           GRFTETVARRYFQQLVSALKFCH+NGVVHRDVKPQNLLLDEQGNLK+SDFGLSALPE+L+
Sbjct: 121 GRFTETVARRYFQQLVSALKFCHENGVVHRDVKPQNLLLDEQGNLKISDFGLSALPEKLK 180

Query: 181 DGLLHTSCGTPAYSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMY 240
           D LLHT+CGTPAYSAPEVM  R   GYDGGKADAWSCGVILFVMLSGFLPF++SNLAAMY
Sbjct: 181 DELLHTACGTPAYSAPEVMIRRGNRGYDGGKADAWSCGVILFVMLSGFLPFEESNLAAMY 240

Query: 241 RKIHRREFQIPNWVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQ 300
           +KIHRREFQIP W++KPVRFLIYHLLDPNP+TRMSIEALMQNPWFKKSL  KQ NE+L+Q
Sbjct: 241 KKIHRREFQIPKWISKPVRFLIYHLLDPNPETRMSIEALMQNPWFKKSLPRKQSNESLMQ 300

Query: 301 SMRDYRMGKERGIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVRE 360
           SM++YR+GKERGIGEMNAFDIISMSSGLDLSGLFETTE+R ERR+ SRATV EVE+RVRE
Sbjct: 301 SMKNYRLGKERGIGEMNAFDIISMSSGLDLSGLFETTEKRAERRFTSRATVREVEERVRE 360

Query: 361 MGVELEYEVE--VGGKGGSIGMGKRGVVVVVEVVEVAVA---LTMVEVRVVKGGEEFEEH 420
           +G  L YEVE     KGG+IGMGK  VVVVVEVVE+AVA   LTMVEVRV+KGGEEFEE 
Sbjct: 361 IGGVLGYEVEAVAVAKGGAIGMGKGRVVVVVEVVEMAVAPVTLTMVEVRVIKGGEEFEEL 420

Query: 421 HWRCFEGKLQGFAVPWQRKCNL 438
           HWRCFE KL+GFAVPWQ KCNL
Sbjct: 421 HWRCFEAKLEGFAVPWQMKCNL 439

BLAST of CSPI03G12600 vs. TAIR 10
Match: AT3G23000.1 (CBL-interacting protein kinase 7 )

HSP 1 Score: 459.9 bits (1182), Expect = 2.3e-129
Identity = 240/423 (56.74%), Postives = 314/423 (74.23%), Query Frame = 0

Query: 14  TPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIGAVMERCIV 73
           +P  TPA  LL KY+LGR LG GSFAKV+ A S+  +  VA+KII+K KTI + ME  I+
Sbjct: 12  SPATTPAKILLGKYELGRRLGSGSFAKVHLARSIESDELVAVKIIEKKKTIESGMEPRII 71

Query: 74  REVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRRGRFTETVARRYFQ 133
           RE+ AMRRL HHPNILKIHEVMATKSKIYLV+E A GGEL +K+ RRGR  E+ ARRYFQ
Sbjct: 72  REIDAMRRLRHHPNILKIHEVMATKSKIYLVMELASGGELFSKVLRRGRLPESTARRYFQ 131

Query: 134 QLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLRDGLLHTSCGTPAY 193
           QL SAL+F H +GV HRDVKPQNLLLDEQGNLKVSDFGLSALPE L++GLLHT+CGTPAY
Sbjct: 132 QLASALRFSHQDGVAHRDVKPQNLLLDEQGNLKVSDFGLSALPEHLQNGLLHTACGTPAY 191

Query: 194 SAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPNW 253
           +APEV++ R   GYDG KADAWSCGVILFV+L G +PFDDSN+AAMYRKIHRR+++ P+W
Sbjct: 192 TAPEVISRR---GYDGAKADAWSCGVILFVLLVGDVPFDDSNIAAMYRKIHRRDYRFPSW 251

Query: 254 VTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQSMRDYRMGKERGI 313
           ++K  + +IY +LDPNP TRMSIE +M+  WFKKSL   + + N+     D  +  +  +
Sbjct: 252 ISKQAKSIIYQMLDPNPVTRMSIETVMKTNWFKKSLETSEFHRNVF----DSEVEMKSSV 311

Query: 314 GEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVREMGVELEYEV---- 373
             + AFD+IS+SSGLDLSGLFE  +++ ERR+ ++ + +EVE++ + +G +L Y V    
Sbjct: 312 NSITAFDLISLSSGLDLSGLFEAKKKK-ERRFTAKVSGVEVEEKAKMIGEKLGYVVKKKM 371

Query: 374 -EVGGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEHHWRCFEGKLQGFA 432
            +  G+   +G+G+   V+VVE VE+ V + +VEV+VV+G E  ++  W     +L+   
Sbjct: 372 MKKEGEVKVVGLGRGRTVIVVEAVELTVDVVVVEVKVVEGEE--DDSRWSDLITELEDIV 424

BLAST of CSPI03G12600 vs. TAIR 10
Match: AT4G14580.1 (CBL-interacting protein kinase 4 )

HSP 1 Score: 448.7 bits (1153), Expect = 5.2e-126
Identity = 237/422 (56.16%), Postives = 306/422 (72.51%), Query Frame = 0

Query: 14  TPTPTPATTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIGAVMERCIV 73
           +P     T LL KY+LGR LG GSFAKV+ A S++    VAIKIIDK KTI + ME  I+
Sbjct: 8   SPEKITGTVLLGKYELGRRLGSGSFAKVHVARSISTGELVAIKIIDKQKTIDSGMEPRII 67

Query: 74  REVAAMRRLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRRGRFTETVARRYFQ 133
           RE+ AMRRL +HPN+LKIHEVMATKSKIYLVVE+A GGEL  K+ R GR  E+ ARRYFQ
Sbjct: 68  REIEAMRRLHNHPNVLKIHEVMATKSKIYLVVEYAAGGELFTKLIRFGRLNESAARRYFQ 127

Query: 134 QLVSALKFCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPE-QLRDGLLHTSCGTPA 193
           QL SAL FCH +G+ HRDVKPQNLLLD+QGNLKVSDFGLSALPE +  +GLLHT+CGTPA
Sbjct: 128 QLASALSFCHRDGIAHRDVKPQNLLLDKQGNLKVSDFGLSALPEHRSNNGLLHTACGTPA 187

Query: 194 YSAPEVMTCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPN 253
           Y+APEV+  R   GYDG KADAWSCGV LFV+L+G++PFDD+N+ AMYRKIH+R+++ P+
Sbjct: 188 YTAPEVIAQR---GYDGAKADAWSCGVFLFVLLAGYVPFDDANIVAMYRKIHKRDYRFPS 247

Query: 254 WVTKPVRFLIYHLLDPNPKTRMSIEALMQNPWFKKSLHLKQGNENLLQSMRDYRMGKERG 313
           W++KP R +IY LLDPNP+TRMSIEA+M   WF+KSL + +   ++ +  R +   + + 
Sbjct: 248 WISKPARSIIYKLLDPNPETRMSIEAVMGTVWFQKSLEISEFQSSVFELDR-FLEKEAKS 307

Query: 314 IGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVREMGVELEYEVEVG 373
              + AFD+IS+SSGLDLSGLFE  +R+ E+R+ +R +   V ++   +G +L + VE  
Sbjct: 308 SNAITAFDLISLSSGLDLSGLFERRKRK-EKRFTARVSAERVVEKAGMIGEKLGFRVEKK 367

Query: 374 GKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFEEH---HWRCFEGKLQGFAV 432
            +   +G+GK    VVVEVVE A  L + +V+VV  GEE EE    HW     +L+   +
Sbjct: 368 EETKVVGLGKGRTAVVVEVVEFAEGLVVADVKVVVEGEEEEEEVESHWSELIVELEEIVL 424

BLAST of CSPI03G12600 vs. TAIR 10
Match: AT5G01810.1 (CBL-interacting protein kinase 15 )

HSP 1 Score: 351.3 bits (900), Expect = 1.1e-96
Identity = 197/402 (49.00%), Postives = 266/402 (66.17%), Query Frame = 0

Query: 21  TTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIGAVMERCIVREVAAMR 80
           + L+ +Y++G+ LG+G+FAKVY A  L    +VAIK+IDK + +   M   I RE++AMR
Sbjct: 6   SVLMLRYEVGKFLGQGTFAKVYHARHLKTGDSVAIKVIDKERILKVGMTEQIKREISAMR 65

Query: 81  RLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRRGRFTETVARRYFQQLVSALK 140
            L  HPNI+++HEVMATKSKIY V+E   GGEL  K+S  G+  E VAR+YFQQLV A+ 
Sbjct: 66  LL-RHPNIVELHEVMATKSKIYFVMEHVKGGELFNKVS-TGKLREDVARKYFQQLVRAVD 125

Query: 141 FCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR-DGLLHTSCGTPAYSAPEVM 200
           FCH  GV HRD+KP+NLLLDE GNLK+SDFGLSAL +  R DGLLHT+CGTPAY APEV+
Sbjct: 126 FCHSRGVCHRDLKPENLLLDEHGNLKISDFGLSALSDSRRQDGLLHTTCGTPAYVAPEVI 185

Query: 201 TCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPNWVTKPVR 260
              S +GYDG KAD WSCGVILFV+L+G+LPF DSNL  +Y+KI + E + PNW+    +
Sbjct: 186 ---SRNGYDGFKADVWSCGVILFVLLAGYLPFRDSNLMELYKKIGKAEVKFPNWLAPGAK 245

Query: 261 FLIYHLLDPNPKTRMSIEALMQNPWFKKSL--HLKQGNEN------LLQSMRDYRMGKER 320
            L+  +LDPNP TR+S E +M++ WF+K L   +K+  E         +        K+R
Sbjct: 246 RLLKRILDPNPNTRVSTEKIMKSSWFRKGLQEEVKESVEEETEVDAEAEGNASAEKEKKR 305

Query: 321 GIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVREMGVELEYEVEV 380
            I  +NAF+IIS+S+G DLSGLFE  E + E R+ S     E+ +++ E+G +L+ +V  
Sbjct: 306 CI-NLNAFEIISLSTGFDLSGLFEKGEEKEEMRFTSNREASEITEKLVEIGKDLKMKVRK 365

Query: 381 GGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFE 414
                 + M     VV  EV E+A +  MV ++   GG+  E
Sbjct: 366 KEHEWRVKMSAEATVVEAEVFEIAPSYHMVVLK-KSGGDTAE 400

BLAST of CSPI03G12600 vs. TAIR 10
Match: AT5G01810.2 (CBL-interacting protein kinase 15 )

HSP 1 Score: 351.3 bits (900), Expect = 1.1e-96
Identity = 197/402 (49.00%), Postives = 266/402 (66.17%), Query Frame = 0

Query: 21  TTLLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIGAVMERCIVREVAAMR 80
           + L+ +Y++G+ LG+G+FAKVY A  L    +VAIK+IDK + +   M   I RE++AMR
Sbjct: 6   SVLMLRYEVGKFLGQGTFAKVYHARHLKTGDSVAIKVIDKERILKVGMTEQIKREISAMR 65

Query: 81  RLDHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRRGRFTETVARRYFQQLVSALK 140
            L  HPNI+++HEVMATKSKIY V+E   GGEL  K+S  G+  E VAR+YFQQLV A+ 
Sbjct: 66  LL-RHPNIVELHEVMATKSKIYFVMEHVKGGELFNKVS-TGKLREDVARKYFQQLVRAVD 125

Query: 141 FCHDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR-DGLLHTSCGTPAYSAPEVM 200
           FCH  GV HRD+KP+NLLLDE GNLK+SDFGLSAL +  R DGLLHT+CGTPAY APEV+
Sbjct: 126 FCHSRGVCHRDLKPENLLLDEHGNLKISDFGLSALSDSRRQDGLLHTTCGTPAYVAPEVI 185

Query: 201 TCRSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPNWVTKPVR 260
              S +GYDG KAD WSCGVILFV+L+G+LPF DSNL  +Y+KI + E + PNW+    +
Sbjct: 186 ---SRNGYDGFKADVWSCGVILFVLLAGYLPFRDSNLMELYKKIGKAEVKFPNWLAPGAK 245

Query: 261 FLIYHLLDPNPKTRMSIEALMQNPWFKKSL--HLKQGNEN------LLQSMRDYRMGKER 320
            L+  +LDPNP TR+S E +M++ WF+K L   +K+  E         +        K+R
Sbjct: 246 RLLKRILDPNPNTRVSTEKIMKSSWFRKGLQEEVKESVEEETEVDAEAEGNASAEKEKKR 305

Query: 321 GIGEMNAFDIISMSSGLDLSGLFETTERRVERRYASRATVMEVEKRVREMGVELEYEVEV 380
            I  +NAF+IIS+S+G DLSGLFE  E + E R+ S     E+ +++ E+G +L+ +V  
Sbjct: 306 CI-NLNAFEIISLSTGFDLSGLFEKGEEKEEMRFTSNREASEITEKLVEIGKDLKMKVRK 365

Query: 381 GGKGGSIGMGKRGVVVVVEVVEVAVALTMVEVRVVKGGEEFE 414
                 + M     VV  EV E+A +  MV ++   GG+  E
Sbjct: 366 KEHEWRVKMSAEATVVEAEVFEIAPSYHMVVLK-KSGGDTAE 400

BLAST of CSPI03G12600 vs. TAIR 10
Match: AT5G01820.1 (serine/threonine protein kinase 1 )

HSP 1 Score: 335.5 bits (859), Expect = 6.4e-92
Identity = 192/393 (48.85%), Postives = 260/393 (66.16%), Query Frame = 0

Query: 23  LLRKYQLGRLLGRGSFAKVYQAVSLADNSTVAIKIIDKSKTIGAVMERCIVREVAAMRRL 82
           L  KY++G+L+G G+FAKVY   S A   +VAIK++ K +     +   I RE+A M RL
Sbjct: 18  LFGKYEVGKLVGCGAFAKVYHGRSTATGQSVAIKVVSKQRLQKGGLNGNIQREIAIMHRL 77

Query: 83  DHHPNILKIHEVMATKSKIYLVVEFAGGGELLAKISRRGRFTETVARRYFQQLVSALKFC 142
             HP+I+++ EV+ATKSKI+ V+EFA GGEL AK+S +GRF E ++RRYFQQL+SA+ +C
Sbjct: 78  -RHPSIVRLFEVLATKSKIFFVMEFAKGGELFAKVS-KGRFCEDLSRRYFQQLISAVGYC 137

Query: 143 HDNGVVHRDVKPQNLLLDEQGNLKVSDFGLSALPEQLR-DGLLHTSCGTPAYSAPEVMTC 202
           H  G+ HRD+KP+NLLLDE+ +LK+SDFGLSAL +Q+R DGLLHT CGTPAY APEV+  
Sbjct: 138 HSRGIFHRDLKPENLLLDEKLDLKISDFGLSALTDQIRPDGLLHTLCGTPAYVAPEVL-- 197

Query: 203 RSGSGYDGGKADAWSCGVILFVMLSGFLPFDDSNLAAMYRKIHRREFQIPNWVTKPVRFL 262
            +  GYDG K D WSCG+ILFV+ +G+LPF+D NL  MYRKI++ EF+IP W +  +R L
Sbjct: 198 -AKKGYDGAKIDIWSCGIILFVLNAGYLPFNDHNLMVMYRKIYKGEFRIPKWTSPDLRRL 257

Query: 263 IYHLLDPNPKTRMSIEALMQNPWFKK-------SLHLKQGNENLLQSMRDYRMGKERGIG 322
           +  LLD NP+TR++IE ++ +PWFK+         HL+  +  L     D  MG  R   
Sbjct: 258 LTRLLDTNPQTRITIEEIIHDPWFKQGYDDRMSKFHLEDSDMKLPADETDSEMGARR--- 317

Query: 323 EMNAFDIISMSSGLDLSGLFETTER--RVERRYASRATVMEVEKRVREMGVELEYEVEVG 382
            MNAFDIIS S G +LSGLF    +  RVE R+ S  T   V +R+ E+       V   
Sbjct: 318 -MNAFDIISGSPGFNLSGLFGDARKYDRVE-RFVSAWTAERVVERLEEIVSAENLTVAKK 377

Query: 383 GKGGSIGMGKRG-VVVVVEVVEVAVALTMVEVR 405
              G    G++G   +VVE+ ++   L M+EVR
Sbjct: 378 ETWGMKIEGQKGNFAMVVEINQLTDELVMIEVR 400

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XIW03.2e-12856.74CBL-interacting serine/threonine-protein kinase 7 OS=Arabidopsis thaliana OX=370... [more]
Q9SUL77.3e-12556.16CBL-interacting serine/threonine-protein kinase 4 OS=Arabidopsis thaliana OX=370... [more]
Q75GK42.8e-10052.07CBL-interacting protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
Q2QMI03.7e-10050.00CBL-interacting protein kinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
P929371.6e-9549.00CBL-interacting serine/threonine-protein kinase 15 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A0A0L5B51.5e-24298.40Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_3... [more]
A0A5D3D0F17.6e-22692.27Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3AXF78.5e-22591.82Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1E9416.3e-20483.26Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IS987.0e-20382.81Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_004134430.23.2e-24298.40CBL-interacting serine/threonine-protein kinase 7 [Cucumis sativus] >KGN56948.2 ... [more]
KAA0049313.11.6e-22592.27CBL-interacting serine/threonine-protein kinase 7-like [Cucumis melo var. makuwa... [more]
XP_008438596.21.7e-22491.82PREDICTED: LOW QUALITY PROTEIN: CBL-interacting serine/threonine-protein kinase ... [more]
XP_038900771.11.4e-21386.36CBL-interacting serine/threonine-protein kinase 7-like [Benincasa hispida][more]
XP_023527173.11.3e-20383.26CBL-interacting serine/threonine-protein kinase 7-like [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
AT3G23000.12.3e-12956.74CBL-interacting protein kinase 7 [more]
AT4G14580.15.2e-12656.16CBL-interacting protein kinase 4 [more]
AT5G01810.11.1e-9649.00CBL-interacting protein kinase 15 [more]
AT5G01810.21.1e-9649.00CBL-interacting protein kinase 15 [more]
AT5G01820.16.4e-9248.85serine/threonine protein kinase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 27..285
e-value: 3.4E-99
score: 345.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 27..285
e-value: 6.5E-68
score: 228.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 27..285
score: 50.378536
IPR004041NAF domainPFAMPF03822NAFcoord: 316..370
e-value: 4.6E-15
score: 55.5
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 312..428
e-value: 4.5E-23
score: 83.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 23..110
e-value: 8.6E-24
score: 85.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 111..308
e-value: 1.1E-56
score: 193.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR24343SERINE/THREONINE KINASEcoord: 18..433
NoneNo IPR availablePANTHERPTHR24343:SF413NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 18..433
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 147..159
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 33..56
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 311..335
score: 12.878716
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 24..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G12600.1CSPI03G12600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity