CSPI03G10430 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G10430
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr3: 8346170 .. 8348071 (+)
RNA-Seq ExpressionCSPI03G10430
SyntenyCSPI03G10430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACAAAAAATAGCATGAATAAATGGAAAAAGAAAATTATGGTTCCAAGGGATTGTCTTACCTAAATTCACCAACTTTAATTTCTTAGAATTCACTTAGCTTTTATCAAACCCACACCTACACTCATCGTCCTTTCCTTTGTCAATGGAAAACTTGATATCAAAACCTAAAGTCTCACACAAGGAATTTAGACAGGCCTAATATTGACACCATTTCTTTGTTGAAGACATGTTTGTGAAGTAGAAGGAAGTTGAAAAGGGAAAAGTAAAAGGAAAGGACAAGAATTAAGAAAAAAGAAAAAGTAGAGAGAGAGAGGATAAATGAGATATAACAATTTCTTACCTCCTCCCACTTTCCTTCCCTTTGCATCAAAGCCCCTAATTAAGCTTTCTTTTTCTTCTGCCCTGCATCTTCAATCAAACTCTCTTTCTACCCCATAAACACATGAAAAAAGAGAAAATAGAGGTACCTGCAAGATCTACAAGTGGTAGAGGAAAATGGGTTCAGTAGGAAACTCAAATCAATGGCAGCCATATGCTTACAAAGATTGTTCTTTAGAGATTTGCAGCATATATTGTCCTCAATGGTGTTACATCATCTTTCCACCACCTCCACCTTTTGGCTATGGCAGCAATGGAGATTCTGCCACTGATTTCTCTCCTCTTATCATTGCTATCATTGGTATTTTGGCTAGTGCCTTCATTTTGGTAAGCTATTACACCATCATCTCCAAGTACTGCAGGAACAGAGCTTCCACCAGCAACGACGCCATGGAAATGGAGGATGAAGAAAATATTTCCCAAATCCGTCATGAAAACCAGCTCCAAGCTCCTCCTCTTCCTCCTCCTGGCTTGGATGAAGCTCTCATCAAGTCCATCACAGTCTGCAAGTACAAAAGAGGAGATGGGTTAGTTGAAGGCACTGATTGCTCAGTTTGCTTGAGTGAGTTTCAAGAAAATGAGAGCTTAAGATTGCTCCCAAAGTGTAGCCATGCTTTTCATCTTCCTTGCATTGACACATGGCTTAAATCTCACTCGACTTGCCCTCTTTGCCGCTCCAATATTTCACCAACAAATCTTTTCTCAACCCCAACTCAAGAAATTCAAACAACACAACATTTTGTTTCATCTGCTTTTCAGTATCAGCATCAGCACAGAACTAATGACACAATAGTGGTTGTAGTGGTTCAAGATTTGGATGATTTAACAGTAGTGAGGCAAGAAACTGTTGATTCTAGGCTTGAAAATGATGATGCTTCTTCCAAGAATCAAAGGGAAGGATGTGCAGCAGAGAGTCAAATTTCAGGGGCAAGAGAAAGAATGAATCAAGTTAGACAGGAATATGATGTTGTTGTGGATGGTGTTGTTGAGCCATTTAGAAGGTCAGTTTCCTTGAATTCTTTATCATGGCAAGGGCAGGTTTCTGTTGCTGATATATTGAGAGTCAGTCAAGATAGTGAGGAAGAGGCAGAGGAAGATGAATTACAGCAAATGGGGATTGGATCTTCAAAAGTATTTGTTCAAGAGCAATCCCATTCCAATCACAGAACTGGGGTTTCCAACTTGGGAATGAATAGATCTATTTCAACTGGGAAACTGGGTTTTACAAATTATGGGAAAGGAAAGAGTTGCATCATTTCCAGTTGAAAGTAGATTTACAAACTGTACAAAATTTGAAAAATTTTCACTCTTACTTCTGTAATATATTGGCGAAATCAAAGAAGAAAACCCAGATTCATACAAGATTAGCAGACTAGATGGACTGAATTCGAATGTACATTTTACGATTTACAGGCCTCTTCAGAGATGTTACTCATGCATCATATTCTGTCATCTATTAAAGAGTTCTCTTTTTTTTTTTGGTTGGATTTTGCTGATGGTAACTGACCAGAATTGTCTGAACT

mRNA sequence

TACAAAAAATAGCATGAATAAATGGAAAAAGAAAATTATGGTTCCAAGGGATTGTCTTACCTAAATTCACCAACTTTAATTTCTTAGAATTCACTTAGCTTTTATCAAACCCACACCTACACTCATCGTCCTTTCCTTTGTCAATGGAAAACTTGATATCAAAACCTAAAGTCTCACACAAGGAATTTAGACAGGCCTAATATTGACACCATTTCTTTGTTGAAGACATGTTTGTGAAGTAGAAGGAAGTTGAAAAGGGAAAAGTAAAAGGAAAGGACAAGAATTAAGAAAAAAGAAAAAGTAGAGAGAGAGAGGATAAATGAGATATAACAATTTCTTACCTCCTCCCACTTTCCTTCCCTTTGCATCAAAGCCCCTAATTAAGCTTTCTTTTTCTTCTGCCCTGCATCTTCAATCAAACTCTCTTTCTACCCCATAAACACATGAAAAAAGAGAAAATAGAGGTACCTGCAAGATCTACAAGTGGTAGAGGAAAATGGGTTCAGTAGGAAACTCAAATCAATGGCAGCCATATGCTTACAAAGATTGTTCTTTAGAGATTTGCAGCATATATTGTCCTCAATGGTGTTACATCATCTTTCCACCACCTCCACCTTTTGGCTATGGCAGCAATGGAGATTCTGCCACTGATTTCTCTCCTCTTATCATTGCTATCATTGGTATTTTGGCTAGTGCCTTCATTTTGGTAAGCTATTACACCATCATCTCCAAGTACTGCAGGAACAGAGCTTCCACCAGCAACGACGCCATGGAAATGGAGGATGAAGAAAATATTTCCCAAATCCGTCATGAAAACCAGCTCCAAGCTCCTCCTCTTCCTCCTCCTGGCTTGGATGAAGCTCTCATCAAGTCCATCACAGTCTGCAAGTACAAAAGAGGAGATGGGTTAGTTGAAGGCACTGATTGCTCAGTTTGCTTGAGTGAGTTTCAAGAAAATGAGAGCTTAAGATTGCTCCCAAAGTGTAGCCATGCTTTTCATCTTCCTTGCATTGACACATGGCTTAAATCTCACTCGACTTGCCCTCTTTGCCGCTCCAATATTTCACCAACAAATCTTTTCTCAACCCCAACTCAAGAAATTCAAACAACACAACATTTTGTTTCATCTGCTTTTCAGTATCAGCATCAGCACAGAACTAATGACACAATAGTGGTTGTAGTGGTTCAAGATTTGGATGATTTAACAGTAGTGAGGCAAGAAACTGTTGATTCTAGGCTTGAAAATGATGATGCTTCTTCCAAGAATCAAAGGGAAGGATGTGCAGCAGAGAGTCAAATTTCAGGGGCAAGAGAAAGAATGAATCAAGTTAGACAGGAATATGATGTTGTTGTGGATGGTGTTGTTGAGCCATTTAGAAGGTCAGTTTCCTTGAATTCTTTATCATGGCAAGGGCAGGTTTCTGTTGCTGATATATTGAGAGTCAGTCAAGATAGTGAGGAAGAGGCAGAGGAAGATGAATTACAGCAAATGGGGATTGGATCTTCAAAAGTATTTGTTCAAGAGCAATCCCATTCCAATCACAGAACTGGGGTTTCCAACTTGGGAATGAATAGATCTATTTCAACTGGGAAACTGGGTTTTACAAATTATGGGAAAGGAAAGAGTTGCATCATTTCCAGTTGAAAGTAGATTTACAAACTGTACAAAATTTGAAAAATTTTCACTCTTACTTCTGTAATATATTGGCGAAATCAAAGAAGAAAACCCAGATTCATACAAGATTAGCAGACTAGATGGACTGAATTCGAATGTACATTTTACGATTTACAGGCCTCTTCAGAGATGTTACTCATGCATCATATTCTGTCATCTATTAAAGAGTTCTCTTTTTTTTTTTGGTTGGATTTTGCTGATGGTAACTGACCAGAATTGTCTGAACT

Coding sequence (CDS)

ATGGGTTCAGTAGGAAACTCAAATCAATGGCAGCCATATGCTTACAAAGATTGTTCTTTAGAGATTTGCAGCATATATTGTCCTCAATGGTGTTACATCATCTTTCCACCACCTCCACCTTTTGGCTATGGCAGCAATGGAGATTCTGCCACTGATTTCTCTCCTCTTATCATTGCTATCATTGGTATTTTGGCTAGTGCCTTCATTTTGGTAAGCTATTACACCATCATCTCCAAGTACTGCAGGAACAGAGCTTCCACCAGCAACGACGCCATGGAAATGGAGGATGAAGAAAATATTTCCCAAATCCGTCATGAAAACCAGCTCCAAGCTCCTCCTCTTCCTCCTCCTGGCTTGGATGAAGCTCTCATCAAGTCCATCACAGTCTGCAAGTACAAAAGAGGAGATGGGTTAGTTGAAGGCACTGATTGCTCAGTTTGCTTGAGTGAGTTTCAAGAAAATGAGAGCTTAAGATTGCTCCCAAAGTGTAGCCATGCTTTTCATCTTCCTTGCATTGACACATGGCTTAAATCTCACTCGACTTGCCCTCTTTGCCGCTCCAATATTTCACCAACAAATCTTTTCTCAACCCCAACTCAAGAAATTCAAACAACACAACATTTTGTTTCATCTGCTTTTCAGTATCAGCATCAGCACAGAACTAATGACACAATAGTGGTTGTAGTGGTTCAAGATTTGGATGATTTAACAGTAGTGAGGCAAGAAACTGTTGATTCTAGGCTTGAAAATGATGATGCTTCTTCCAAGAATCAAAGGGAAGGATGTGCAGCAGAGAGTCAAATTTCAGGGGCAAGAGAAAGAATGAATCAAGTTAGACAGGAATATGATGTTGTTGTGGATGGTGTTGTTGAGCCATTTAGAAGGTCAGTTTCCTTGAATTCTTTATCATGGCAAGGGCAGGTTTCTGTTGCTGATATATTGAGAGTCAGTCAAGATAGTGAGGAAGAGGCAGAGGAAGATGAATTACAGCAAATGGGGATTGGATCTTCAAAAGTATTTGTTCAAGAGCAATCCCATTCCAATCACAGAACTGGGGTTTCCAACTTGGGAATGAATAGATCTATTTCAACTGGGAAACTGGGTTTTACAAATTATGGGAAAGGAAAGAGTTGCATCATTTCCAGTTGA

Protein sequence

MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAIIGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVRQETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLNSLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNRSISTGKLGFTNYGKGKSCIISS*
Homology
BLAST of CSPI03G10430 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 6.7e-66
Identity = 173/394 (43.91%), Postives = 217/394 (55.08%), Query Frame = 0

Query: 1   MGSVGNSNQWQPY--AYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGD-SATDFSPLI 60
           MGS GN N W     +Y+DCS  +CS+YCPQWCY+IFPPPP F      D S++DFSPL+
Sbjct: 1   MGSTGNPNPWGTTYDSYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLL 60

Query: 61  IAIIGILASAFILVSYYTIISKYC----RNRASTSNDAMEMEDEENISQIRHENQLQAPP 120
           IA+IGILASAFILVSYYT+ISKYC     N +STS  A+     +   Q  + N      
Sbjct: 61  IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGAT 120

Query: 121 LP--------PPGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSH 180
            P          GLDE+LIKSITV KY++ DG VE +DCSVCLSEFQENESLRLLPKC+H
Sbjct: 121 NPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNH 180

Query: 181 AFHLPCIDTWLKSHSTCPLCRSNI---SPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTN 240
           AFH+PCIDTWLKSHS CPLCR+ I   S   +     Q+I T  + +S          T 
Sbjct: 181 AFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSIS----------TG 240

Query: 241 DTIVVVVVQDLDDLTVVRQETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEY 300
           D  VVVV  DL++ +  R ETV+          ++ R+G                     
Sbjct: 241 DDSVVVVNLDLEN-SRSRNETVNEGSTPKPPEMQDSRDG--------------------- 300

Query: 301 DVVVDGVVEPFRRSVSLNSLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQ 360
                      RRS SLN  S  G VS+ADILR  +D EE A        G+G+S+ +V+
Sbjct: 301 ---------EERRSASLN--SGGGVVSIADILREIEDDEESA--------GVGTSR-WVE 342

Query: 361 E----------QSHSNHRTGVSNLGMNRSISTGK 367
           E           S +N   G+SN  +  S++  K
Sbjct: 361 EGEGEKTPPPSGSAANQTNGISNFLVRSSMAAMK 342

BLAST of CSPI03G10430 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.2e-65
Identity = 166/392 (42.35%), Postives = 227/392 (57.91%), Query Frame = 0

Query: 6   NSNQWQPY-AYKDCSLEICSIYCPQWCYIIFPPPPP--FGYGSNGDSATDFSPLIIAIIG 65
           N N W PY +Y DCS  IC+IYCPQWCY+IFPPPPP  F    +  S++ FSPL+IA+IG
Sbjct: 4   NPNPWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIG 63

Query: 66  ILASAFILVSYYTIISKYC-RNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLDE 125
           IL SA ILVSYYT+ISKYC R+  ++S++ + +    N +     +  Q       GL+E
Sbjct: 64  ILTSALILVSYYTLISKYCHRHHQTSSSETLNL----NHNGEGFFSSTQRISTNGDGLNE 123

Query: 126 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHST 185
           ++IKSITV KYK GDG V+G+DCSVCLSEF+ENESLRLLPKC+HAFHLPCIDTWLKSHS 
Sbjct: 124 SMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSN 183

Query: 186 CPLCRSNISPTNLFSTPTQEI-QTTQHFVSSAFQYQHQHRTNDTIVVVV------VQDLD 245
           CPLCR+ ++  N    PT  + Q     V++     HQ  +   I + +        D D
Sbjct: 184 CPLCRAFVTGVN---NPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFD 243

Query: 246 DLTVVRQETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFR 305
            + V+    + SR  N DA S+ Q              E   + + E  +       P R
Sbjct: 244 SVVVIEDLEIGSR--NSDARSELQ------------LPEERRETKDEDSL-------PIR 303

Query: 306 RSVSLNSLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQ---------S 365
           RSVSLNS      VS+AD+LR       E E++E +  G+G+S+   + +         +
Sbjct: 304 RSVSLNS---GVVVSIADVLR-------EIEDEEGESGGVGTSQRREEGEDGDGKTIPPT 357

Query: 366 HSNHRTGVSNLGMNRSISTGKLGFTNYGKGKS 378
            +N R+G  +    RS+STG+  F+ Y +G++
Sbjct: 364 EANQRSGGVSGFFVRSLSTGRFIFSRYDRGRN 357

BLAST of CSPI03G10430 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 223.4 bits (568), Expect = 4.4e-57
Identity = 151/346 (43.64%), Postives = 189/346 (54.62%), Query Frame = 0

Query: 10  WQPY-AYKDCSLEICSIYCPQWCYIIFPPPPPF---GYGSNGDSATDFSPLIIAIIGILA 69
           W PY   +DCS  +CS+YCPQWCY IFPPPPPF   G  ++  S   FSPL+IAIIG+LA
Sbjct: 8   WVPYEPTRDCSQGLCSMYCPQWCYFIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGVLA 67

Query: 70  SAFILVSYYTIISKYC------RNRASTSNDAMEMEDEENISQIRH-----ENQLQAPPL 129
           SAF+LVSYYT ISKYC      R R   S+           S  RH      +       
Sbjct: 68  SAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRSHESWNVS 127

Query: 130 PPPGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDT 189
           PP GLDE LI  ITVCKY+RGDG V  TDCSVCL EF + ESLRLLP+CSHAFH  CIDT
Sbjct: 128 PPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDT 187

Query: 190 WLKSHSTCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLD 249
           WLKSHS CPLCR+NI+    F T        +          + H      VVVV+  L+
Sbjct: 188 WLKSHSNCPLCRANIT----FVTVGLASPEPEGCAPGETGGDNTHE-----VVVVMDGLE 247

Query: 250 DLTVVRQETVD-SRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPF 309
           +L   +QE V  +   +DD  +K+  EG     + +GA E    +R+E          P 
Sbjct: 248 NLCEEQQEAVSRASTADDDHDAKDVAEGM---EEANGAAE----IREE--------GSPP 307

Query: 310 RRSVSLNSLSWQGQVSVADILRVSQDSE-EEAEEDELQQMGIGSSK 339
           +R  S   L    ++ +AD+L+ S + E   A E  L   G G+S+
Sbjct: 308 KRGASSFDLHRDNRMCIADVLQESMEDELTAARESGLLAGGAGTSR 329

BLAST of CSPI03G10430 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 2.2e-40
Identity = 93/204 (45.59%), Postives = 117/204 (57.35%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAY-KDCSLEICSIYCPQWCYIIFPPPPPFGY----GSNGDSATDFSP 60
           M S  N + W  Y   +DC+  +CS +CPQWC  I   PPP  Y         S  + SP
Sbjct: 1   MESDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSP 60

Query: 61  LIIAIIGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLP 120
           L+IAI GI A+AF+L +YYT++SKYC N   T+N+A       +I  I   N  +     
Sbjct: 61  LVIAIFGIFATAFLLAAYYTLVSKYCAN--DTTNEAASESGRSDI--ILDVNSPERGDQD 120

Query: 121 PP--------GLDEALIKSITVCKYKRGDG--LVEGTDCSVCLSEFQENESLRLLPKCSH 180
            P        GLD+ LIK I   K K+      + GTDCS+CL EF E+ESLRLLPKC+H
Sbjct: 121 DPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNH 180

Query: 181 AFHLPCIDTWLKSHSTCPLCRSNI 190
            FH+ CID WLKSHS CPLCR+ I
Sbjct: 181 TFHVVCIDRWLKSHSNCPLCRAKI 200

BLAST of CSPI03G10430 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 1.1e-31
Identity = 78/204 (38.24%), Postives = 111/204 (54.41%), Query Frame = 0

Query: 17  DCSLEICSIYCPQWC------YIIFPPPPPFG----------------YGSNGDSATDFS 76
           DCS  +C   CP  C      Y I P  PP+                 Y  + DS++   
Sbjct: 22  DCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLD 81

Query: 77  PL-IIAIIGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPP 136
            + II I G + +  IL++ + +++K+  +  +  N      D E+   +  E       
Sbjct: 82  AISIITITGAVLA--ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ 141

Query: 137 LPPP-------GLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHA 191
           +  P       GL +++I SIT+C YKRGDGL+E TDC VCL+EF+E+ESLRLLPKC+HA
Sbjct: 142 VDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHA 201

BLAST of CSPI03G10430 vs. ExPASy TrEMBL
Match: A0A0A0L405 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G129520 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 9.7e-217
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR
Sbjct: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300
           QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN
Sbjct: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300

Query: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360
           SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR
Sbjct: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCIISS 383
           SISTGKLGFTNYGKGKSCIISS
Sbjct: 361 SISTGKLGFTNYGKGKSCIISS 382

BLAST of CSPI03G10430 vs. ExPASy TrEMBL
Match: A0A5D3D2D1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003080 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 6.5e-205
Identity = 366/382 (95.81%), Postives = 372/382 (97.38%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEEN+SQIRHENQLQAPPLPPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENLSQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISPTNLFST TQEIQ TQHFVSSAFQYQHQHRTNDT++VVVVQDLDDLTVVR
Sbjct: 181 TCPLCRSNISPTNLFSTATQEIQ-TQHFVSSAFQYQHQHRTNDTVLVVVVQDLDDLTVVR 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300
           QETV SRLENDDASSKNQREG A+ESQ SG RERMNQVRQEYD VVDGVV+PFRRSVSLN
Sbjct: 241 QETVVSRLENDDASSKNQREGYASESQNSGERERMNQVRQEYD-VVDGVVQPFRRSVSLN 300

Query: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360
           SLSWQG VSVADILRVSQDSEEE EEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR
Sbjct: 301 SLSWQGPVSVADILRVSQDSEEEVEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCIISS 383
           SISTGKLGFTNYGKGKSCII S
Sbjct: 361 SISTGKLGFTNYGKGKSCIIPS 380

BLAST of CSPI03G10430 vs. ExPASy TrEMBL
Match: A0A1S3AW58 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483448 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 6.5e-205
Identity = 366/382 (95.81%), Postives = 372/382 (97.38%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEEN+SQIRHENQLQAPPLPPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENLSQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISPTNLFST TQEIQ TQHFVSSAFQYQHQHRTNDT++VVVVQDLDDLTVVR
Sbjct: 181 TCPLCRSNISPTNLFSTATQEIQ-TQHFVSSAFQYQHQHRTNDTVLVVVVQDLDDLTVVR 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300
           QETV SRLENDDASSKNQREG A+ESQ SG RERMNQVRQEYD VVDGVV+PFRRSVSLN
Sbjct: 241 QETVVSRLENDDASSKNQREGYASESQNSGERERMNQVRQEYD-VVDGVVQPFRRSVSLN 300

Query: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360
           SLSWQG VSVADILRVSQDSEEE EEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR
Sbjct: 301 SLSWQGPVSVADILRVSQDSEEEVEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCIISS 383
           SISTGKLGFTNYGKGKSCII S
Sbjct: 361 SISTGKLGFTNYGKGKSCIIPS 380

BLAST of CSPI03G10430 vs. ExPASy TrEMBL
Match: A0A6J1D151 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016068 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.2e-168
Identity = 313/382 (81.94%), Postives = 337/382 (88.22%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYG+NGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRA+T++ AMEM+DEEN++QIRHENQLQA   PP GLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRAATTSAAMEMDDEENLAQIRHENQLQA---PPAGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKY RGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYIRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISPTNLF  PTQEIQ T+H  ++A QY  QHR+NDT+VVVVVQDL DLT V 
Sbjct: 181 TCPLCRSNISPTNLFPPPTQEIQRTEHINAAALQY--QHRSNDTVVVVVVQDLQDLT-VS 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300
           Q+T    +END ASSKNQR+  A+ SQ S  R+RM QVR+  D VV  VV+PFRRSVSL+
Sbjct: 241 QQTAVLGIENDGASSKNQRQSFASVSQDSEPRDRMIQVRE--DDVVGDVVQPFRRSVSLD 300

Query: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360
           S SWQGQVSVADILR SQDSEEE EEDE+ QMGIGSSKVFVQE   SNH+ GVSNLGMNR
Sbjct: 301 SFSWQGQVSVADILRESQDSEEEVEEDEM-QMGIGSSKVFVQEHFQSNHKNGVSNLGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCIISS 383
           S+STG+LGFTNY KGKSCII S
Sbjct: 361 SVSTGRLGFTNYEKGKSCIIPS 373

BLAST of CSPI03G10430 vs. ExPASy TrEMBL
Match: A0A6J1G2W9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111450329 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.8e-162
Identity = 306/383 (79.90%), Postives = 328/383 (85.64%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAY+DCSLEICSIYCPQWCYIIFPPPPPFGYG+N DS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYRDCSLEICSIYCPQWCYIIFPPPPPFGYGANSDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRA+TSN  MEM+DEEN+SQIRHENQLQAPP PP GLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRATTSNSNMEMDDEENLSQIRHENQLQAPP-PPAGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALI SITVCKYKRGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALINSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISP NLF  PTQEIQ T+H   +++ +Q+QHR+NDT+VVVVVQDL DLTV  
Sbjct: 181 TCPLCRSNISPINLFPPPTQEIQRTEHL--NSYNFQYQHRSNDTVVVVVVQDLPDLTVSS 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERM-NQVRQEYDVVVDGVVEPFRRSVSL 300
           QET    LEND  SSKNQR+   +++Q S  R+ M +Q RQ      D  V+PFRRSVSL
Sbjct: 241 QETAVLGLENDGVSSKNQRQSFVSDNQDSEPRDIMIHQTRQ------DDAVQPFRRSVSL 300

Query: 301 NSLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMN 360
           NSLSWQGQVSVADILR SQDSEEE EEDEL QMGIGSSK FVQE  HS     VSNL M+
Sbjct: 301 NSLSWQGQVSVADILRASQDSEEEVEEDEL-QMGIGSSKAFVQEDCHSR----VSNLEMH 360

Query: 361 RSISTGKLGFTNYG--KGKSCII 381
           RSISTGKLGFTNYG  KGKSCII
Sbjct: 361 RSISTGKLGFTNYGNWKGKSCII 369

BLAST of CSPI03G10430 vs. NCBI nr
Match: XP_004134368.1 (RING-H2 finger protein ATL51 [Cucumis sativus] >KGN56695.1 hypothetical protein Csa_010103 [Cucumis sativus])

HSP 1 Score: 762.3 bits (1967), Expect = 2.0e-216
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR
Sbjct: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300
           QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN
Sbjct: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300

Query: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360
           SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR
Sbjct: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCIISS 383
           SISTGKLGFTNYGKGKSCIISS
Sbjct: 361 SISTGKLGFTNYGKGKSCIISS 382

BLAST of CSPI03G10430 vs. NCBI nr
Match: XP_008438300.1 (PREDICTED: RING-H2 finger protein ATL52 [Cucumis melo] >KAA0049120.1 RING-H2 finger protein ATL52 [Cucumis melo var. makuwa] >TYK17444.1 RING-H2 finger protein ATL52 [Cucumis melo var. makuwa])

HSP 1 Score: 723.0 bits (1865), Expect = 1.3e-204
Identity = 366/382 (95.81%), Postives = 372/382 (97.38%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEEN+SQIRHENQLQAPPLPPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENLSQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISPTNLFST TQEIQ TQHFVSSAFQYQHQHRTNDT++VVVVQDLDDLTVVR
Sbjct: 181 TCPLCRSNISPTNLFSTATQEIQ-TQHFVSSAFQYQHQHRTNDTVLVVVVQDLDDLTVVR 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300
           QETV SRLENDDASSKNQREG A+ESQ SG RERMNQVRQEYD VVDGVV+PFRRSVSLN
Sbjct: 241 QETVVSRLENDDASSKNQREGYASESQNSGERERMNQVRQEYD-VVDGVVQPFRRSVSLN 300

Query: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360
           SLSWQG VSVADILRVSQDSEEE EEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR
Sbjct: 301 SLSWQGPVSVADILRVSQDSEEEVEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCIISS 383
           SISTGKLGFTNYGKGKSCII S
Sbjct: 361 SISTGKLGFTNYGKGKSCIIPS 380

BLAST of CSPI03G10430 vs. NCBI nr
Match: XP_038901021.1 (RING-H2 finger protein ATL52-like [Benincasa hispida])

HSP 1 Score: 675.2 bits (1741), Expect = 3.2e-190
Identity = 342/379 (90.24%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSN AMEMEDEEN+SQIRHEN LQAPP PPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPP-PPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISPTNLF  PTQEIQTTQHFVSS F YQHQHRTNDT+VVVVVQDL   TVVR
Sbjct: 181 TCPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVR 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300
           QE  DSRL+ND ASSKNQRE  A++SQ S AR+RMNQVRQEYD V+DGVV PFRRSVSLN
Sbjct: 241 QEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYD-VLDGVVHPFRRSVSLN 300

Query: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360
           SLSW+GQVSVADILRVSQDSEEE EEDELQQMGIGSS+VFVQE S+ NH+ GVSN GMNR
Sbjct: 301 SLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCI 380
           SISTGKLGFTNYGKGKSCI
Sbjct: 361 SISTGKLGFTNYGKGKSCI 377

BLAST of CSPI03G10430 vs. NCBI nr
Match: XP_022147036.1 (RING-H2 finger protein ATL52-like [Momordica charantia])

HSP 1 Score: 601.7 bits (1550), Expect = 4.5e-168
Identity = 313/382 (81.94%), Postives = 337/382 (88.22%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYG+NGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRA+T++ AMEM+DEEN++QIRHENQLQA   PP GLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRAATTSAAMEMDDEENLAQIRHENQLQA---PPAGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKY RGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYIRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISPTNLF  PTQEIQ T+H  ++A QY  QHR+NDT+VVVVVQDL DLT V 
Sbjct: 181 TCPLCRSNISPTNLFPPPTQEIQRTEHINAAALQY--QHRSNDTVVVVVVQDLQDLT-VS 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300
           Q+T    +END ASSKNQR+  A+ SQ S  R+RM QVR+  D VV  VV+PFRRSVSL+
Sbjct: 241 QQTAVLGIENDGASSKNQRQSFASVSQDSEPRDRMIQVRE--DDVVGDVVQPFRRSVSLD 300

Query: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360
           S SWQGQVSVADILR SQDSEEE EEDE+ QMGIGSSKVFVQE   SNH+ GVSNLGMNR
Sbjct: 301 SFSWQGQVSVADILRESQDSEEEVEEDEM-QMGIGSSKVFVQEHFQSNHKNGVSNLGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCIISS 383
           S+STG+LGFTNY KGKSCII S
Sbjct: 361 SVSTGRLGFTNYEKGKSCIIPS 373

BLAST of CSPI03G10430 vs. NCBI nr
Match: XP_023540121.1 (RING-H2 finger protein ATL51-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 586.3 bits (1510), Expect = 2.0e-163
Identity = 308/385 (80.00%), Postives = 331/385 (85.97%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60
           MGSVGNSNQWQPYAY+DCSLEICSIYCPQWCYIIFPPPPPFGYG+N DS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYRDCSLEICSIYCPQWCYIIFPPPPPFGYGTNSDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120
           IGILASAFILVSYYTIISKYCRNRA+TSN  MEM+DEEN+SQIRHENQLQAPP PP GLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRATTSNSNMEMDDEENLSQIRHENQLQAPP-PPAGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240
           TCPLCRSNISP NLF  PTQEIQ T+H   +++ +Q+QHR+NDT+VVVVVQDL DLTV  
Sbjct: 181 TCPLCRSNISPINLFPPPTQEIQRTEHL--NSYNFQYQHRSNDTVVVVVVQDLPDLTVSS 240

Query: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERM-NQVRQEYDVVVDGVVEPFRRSVSL 300
           QET    LEND  SSKNQR+   +++Q S  R+ M +Q+RQ      D  V+PFRRSVSL
Sbjct: 241 QETAVLGLENDGVSSKNQRQSFVSDNQDSEPRDIMIHQIRQ------DDAVQPFRRSVSL 300

Query: 301 NSLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMN 360
           NSLSWQGQVSVADILR SQDSEEE EEDEL QMGIGSSK FVQE  HS     VSNL M+
Sbjct: 301 NSLSWQGQVSVADILRASQDSEEEVEEDEL-QMGIGSSKAFVQEDCHSR----VSNLEMH 360

Query: 361 RSISTGKLGFTNYG--KGKSCIISS 383
           RSISTGKLGFTNYG  KGKSCII S
Sbjct: 361 RSISTGKLGFTNYGNWKGKSCIIPS 371

BLAST of CSPI03G10430 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.8e-67
Identity = 173/394 (43.91%), Postives = 217/394 (55.08%), Query Frame = 0

Query: 1   MGSVGNSNQWQPY--AYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGD-SATDFSPLI 60
           MGS GN N W     +Y+DCS  +CS+YCPQWCY+IFPPPP F      D S++DFSPL+
Sbjct: 1   MGSTGNPNPWGTTYDSYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLL 60

Query: 61  IAIIGILASAFILVSYYTIISKYC----RNRASTSNDAMEMEDEENISQIRHENQLQAPP 120
           IA+IGILASAFILVSYYT+ISKYC     N +STS  A+     +   Q  + N      
Sbjct: 61  IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGAT 120

Query: 121 LP--------PPGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSH 180
            P          GLDE+LIKSITV KY++ DG VE +DCSVCLSEFQENESLRLLPKC+H
Sbjct: 121 NPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNH 180

Query: 181 AFHLPCIDTWLKSHSTCPLCRSNI---SPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTN 240
           AFH+PCIDTWLKSHS CPLCR+ I   S   +     Q+I T  + +S          T 
Sbjct: 181 AFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSIS----------TG 240

Query: 241 DTIVVVVVQDLDDLTVVRQETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEY 300
           D  VVVV  DL++ +  R ETV+          ++ R+G                     
Sbjct: 241 DDSVVVVNLDLEN-SRSRNETVNEGSTPKPPEMQDSRDG--------------------- 300

Query: 301 DVVVDGVVEPFRRSVSLNSLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQ 360
                      RRS SLN  S  G VS+ADILR  +D EE A        G+G+S+ +V+
Sbjct: 301 ---------EERRSASLN--SGGGVVSIADILREIEDDEESA--------GVGTSR-WVE 342

Query: 361 E----------QSHSNHRTGVSNLGMNRSISTGK 367
           E           S +N   G+SN  +  S++  K
Sbjct: 361 EGEGEKTPPPSGSAANQTNGISNFLVRSSMAAMK 342

BLAST of CSPI03G10430 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 251.9 bits (642), Expect = 8.2e-67
Identity = 166/392 (42.35%), Postives = 227/392 (57.91%), Query Frame = 0

Query: 6   NSNQWQPY-AYKDCSLEICSIYCPQWCYIIFPPPPP--FGYGSNGDSATDFSPLIIAIIG 65
           N N W PY +Y DCS  IC+IYCPQWCY+IFPPPPP  F    +  S++ FSPL+IA+IG
Sbjct: 4   NPNPWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIG 63

Query: 66  ILASAFILVSYYTIISKYC-RNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLDE 125
           IL SA ILVSYYT+ISKYC R+  ++S++ + +    N +     +  Q       GL+E
Sbjct: 64  ILTSALILVSYYTLISKYCHRHHQTSSSETLNL----NHNGEGFFSSTQRISTNGDGLNE 123

Query: 126 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHST 185
           ++IKSITV KYK GDG V+G+DCSVCLSEF+ENESLRLLPKC+HAFHLPCIDTWLKSHS 
Sbjct: 124 SMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSN 183

Query: 186 CPLCRSNISPTNLFSTPTQEI-QTTQHFVSSAFQYQHQHRTNDTIVVVV------VQDLD 245
           CPLCR+ ++  N    PT  + Q     V++     HQ  +   I + +        D D
Sbjct: 184 CPLCRAFVTGVN---NPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFD 243

Query: 246 DLTVVRQETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFR 305
            + V+    + SR  N DA S+ Q              E   + + E  +       P R
Sbjct: 244 SVVVIEDLEIGSR--NSDARSELQ------------LPEERRETKDEDSL-------PIR 303

Query: 306 RSVSLNSLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQ---------S 365
           RSVSLNS      VS+AD+LR       E E++E +  G+G+S+   + +         +
Sbjct: 304 RSVSLNS---GVVVSIADVLR-------EIEDEEGESGGVGTSQRREEGEDGDGKTIPPT 357

Query: 366 HSNHRTGVSNLGMNRSISTGKLGFTNYGKGKS 378
            +N R+G  +    RS+STG+  F+ Y +G++
Sbjct: 364 EANQRSGGVSGFFVRSLSTGRFIFSRYDRGRN 357

BLAST of CSPI03G10430 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 1.6e-41
Identity = 93/204 (45.59%), Postives = 117/204 (57.35%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAY-KDCSLEICSIYCPQWCYIIFPPPPPFGY----GSNGDSATDFSP 60
           M S  N + W  Y   +DC+  +CS +CPQWC  I   PPP  Y         S  + SP
Sbjct: 1   MESDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSP 60

Query: 61  LIIAIIGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLP 120
           L+IAI GI A+AF+L +YYT++SKYC N   T+N+A       +I  I   N  +     
Sbjct: 61  LVIAIFGIFATAFLLAAYYTLVSKYCAN--DTTNEAASESGRSDI--ILDVNSPERGDQD 120

Query: 121 PP--------GLDEALIKSITVCKYKRGDG--LVEGTDCSVCLSEFQENESLRLLPKCSH 180
            P        GLD+ LIK I   K K+      + GTDCS+CL EF E+ESLRLLPKC+H
Sbjct: 121 DPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNH 180

Query: 181 AFHLPCIDTWLKSHSTCPLCRSNI 190
            FH+ CID WLKSHS CPLCR+ I
Sbjct: 181 TFHVVCIDRWLKSHSNCPLCRAKI 200

BLAST of CSPI03G10430 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 139.0 bits (349), Expect = 7.7e-33
Identity = 78/204 (38.24%), Postives = 111/204 (54.41%), Query Frame = 0

Query: 17  DCSLEICSIYCPQWC------YIIFPPPPPFG----------------YGSNGDSATDFS 76
           DCS  +C   CP  C      Y I P  PP+                 Y  + DS++   
Sbjct: 22  DCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLD 81

Query: 77  PL-IIAIIGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPP 136
            + II I G + +  IL++ + +++K+  +  +  N      D E+   +  E       
Sbjct: 82  AISIITITGAVLA--ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ 141

Query: 137 LPPP-------GLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHA 191
           +  P       GL +++I SIT+C YKRGDGL+E TDC VCL+EF+E+ESLRLLPKC+HA
Sbjct: 142 VDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHA 201

BLAST of CSPI03G10430 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 131.0 bits (328), Expect = 2.1e-30
Identity = 75/182 (41.21%), Postives = 106/182 (58.24%), Query Frame = 0

Query: 29  QWCYIIFPPPPPFGYGSNGDSATDFSPLIIAIIG-ILASAFILVSYYTIISKYCRNRAS- 88
           ++C+  +PPPPP        S     P  I ++G I+   F++   Y  I++  R  A+ 
Sbjct: 19  RFCFRQYPPPPPPPPPPRELSL--LLPTSICVVGSIILFLFLVFFLYLHITQQRRISAAS 78

Query: 89  -TSNDAMEMEDEENISQIRHENQLQAPPLPPPGLDEALIKSITVCKYKRGDGLVEGTDCS 148
            T  D  + EDE+   +    +      +P  GL  + I SITV  +K+G+G+++GT+CS
Sbjct: 79  VTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECS 138

Query: 149 VCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHSTCPLCRSNISPTNLFSTPTQEIQTT 208
           VCL+EF+E+ESLRLLPKCSHAFHL CIDTWL SH  CPLCR   +P  L + P  +   T
Sbjct: 139 VCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCR---APVLLITEPPHQETET 195

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRQ86.7e-6643.91RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Q9LF641.2e-6542.35RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q7XLY84.4e-5743.64E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
P0C0412.2e-4045.59Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P... [more]
Q8LFY81.1e-3138.24RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0L4059.7e-217100.00RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G129520 PE... [more]
A0A5D3D2D16.5e-20595.81RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AW586.5e-20595.81RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483448 PE=3 ... [more]
A0A6J1D1512.2e-16881.94RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101606... [more]
A0A6J1G2W91.8e-16279.90RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111450329... [more]
Match NameE-valueIdentityDescription
XP_004134368.12.0e-216100.00RING-H2 finger protein ATL51 [Cucumis sativus] >KGN56695.1 hypothetical protein ... [more]
XP_008438300.11.3e-20495.81PREDICTED: RING-H2 finger protein ATL52 [Cucumis melo] >KAA0049120.1 RING-H2 fin... [more]
XP_038901021.13.2e-19090.24RING-H2 finger protein ATL52-like [Benincasa hispida][more]
XP_022147036.14.5e-16881.94RING-H2 finger protein ATL52-like [Momordica charantia][more]
XP_023540121.12.0e-16380.00RING-H2 finger protein ATL51-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G03550.14.8e-6743.91RING/U-box superfamily protein [more]
AT5G17600.18.2e-6742.35RING/U-box superfamily protein [more]
AT4G17905.11.6e-4145.59RING/U-box superfamily protein [more]
AT1G72220.17.7e-3338.24RING/U-box superfamily protein [more]
AT5G10380.12.1e-3041.21RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 144..185
e-value: 2.1E-7
score: 40.6
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 143..186
e-value: 1.4E-13
score: 51.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 144..186
score: 12.961374
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 119..193
e-value: 8.8E-22
score: 78.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..274
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 245..274
NoneNo IPR availablePANTHERPTHR45676:SF108E3 UBIQUITIN-PROTEIN LIGASE OS04G0590900-LIKEcoord: 4..242
coord: 274..374
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 143..186
e-value: 1.75681E-18
score: 76.1865
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 138..192
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 4..242
coord: 274..374

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G10430.1CSPI03G10430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane