CSPI03G10010 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G10010
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr3: 8057247 .. 8058771 (-)
RNA-Seq Expression	CSPI03G10010
Synteny	CSPI03G10010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGAGCGTTTGATCGGAGCTTTGAAGTAAAGAGATTCTTCTATCATCATCAATGGATTCTGATTTTCCTCCTCTTTTTCGATGCCCCATTTCCATGGAGCTCATGGAAGATCCGGTCACTGTCTCTACCGGCGTCACCTACGATAGAAAGAGCATTGAAAAATGGCTTTTTACTTACAATAAAAAAACTTGTCCGGCGACGATGCAGACACTCGCTGCCGGTGCTGAAGACTTCGTCATCACCCCTAATCTGAATCTCAAGCGCCTCATTCTCGCTTGGAAAATCAATGATTCTGCCTTGACGCCGGCGACGGCGCTCGAGGAAGTCTCGTCGCTTCTTGCTACTGTTGAGTCTTCTCCATTCAAGGTTAGTTCTTTGAGGAAATTGAGATCGATGGTTGAAATGAACGATGGGATGAAATCGGAGTTTATTAGATTGAGTGGGATTGGGATTGTTGTTAATGTTGTGATTCAAATTCTTATTGATTGTTCTGATTTCTCGACGTTGGACACTTGTGAAGAGGCTTTGGGCGTTCTGTGTCAATGCGTTGAGGCGCAGCAGTTGACTGGACATCTAGGGGTAAAGCACTGTTTCGGTGCGGGCCGCGAGAGCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGATCCGATTTCAAATGAAGATAAACTGTTCGACGAAATGTATAAGAGAGAAGAATTGATGAAATCGATTGCTGTTGTTGTACAAAGGGGAAGTGCAGAAGGGCGTTTTTACGCTGTGAAAATCCTTAGAAATATTGCTAAAAACACCAATTACAATTGGGGATCTATTATGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCGGACGAATTTCCCACAAAGGCTAGCTCATGTGCATTAGATGTAATGATAGAAATTCTCTCATCCTCGAAAAGGAGTCGAGTGAAGGCGATCGAAGCTGGGGCAGTGTGGATTCTGATCGAGCTTTTACCGGAGTCGGGGCGATCGAAATGCGAGAGAATGCTGCAGATTCTGCAGAGTTTATGTGAATGTGCTGAAGGGAGATTAGCTTTGGTAGAACATGGGATGGGAATTGCAGCTGTGACTAAGAAGATTTTGAATGTTTCATATGTAGCAACAAAAATTGGGGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCAAGGGAAAGGGTTGTAGATGAGATGATGGCATGTGGGGCAGTGAAGAAGCTGCTGATGATACTACACATGGACAGCACCGGTGTCGGTGACGGTGGCCGGAGTTCGACCAAGGAGAAGGTGATCAAGATTTTGAAGATGCATGGGAACAAATGGAGGCGTAGTCCATGTTTTCCTAGTGAGTTGAAGAATTATTTGAAGTTTGTGAATGATGAATGATATTGATCTTTGCTAAAGAAATCAACATACTATAGGAAATAATAATAATAATAATCCGTTTGTAACACATTTTTGCTCAATTTTAATTGAGTTTTGGAAATATATAGTAATTGAATAGGG

mRNA sequence

TTGGAGCGTTTGATCGGAGCTTTGAAGTAAAGAGATTCTTCTATCATCATCAATGGATTCTGATTTTCCTCCTCTTTTTCGATGCCCCATTTCCATGGAGCTCATGGAAGATCCGGTCACTGTCTCTACCGGCGTCACCTACGATAGAAAGAGCATTGAAAAATGGCTTTTTACTTACAATAAAAAAACTTGTCCGGCGACGATGCAGACACTCGCTGCCGGTGCTGAAGACTTCGTCATCACCCCTAATCTGAATCTCAAGCGCCTCATTCTCGCTTGGAAAATCAATGATTCTGCCTTGACGCCGGCGACGGCGCTCGAGGAAGTCTCGTCGCTTCTTGCTACTGTTGAGTCTTCTCCATTCAAGGTTAGTTCTTTGAGGAAATTGAGATCGATGGTTGAAATGAACGATGGGATGAAATCGGAGTTTATTAGATTGAGTGGGATTGGGATTGTTGTTAATGTTGTGATTCAAATTCTTATTGATTGTTCTGATTTCTCGACGTTGGACACTTGTGAAGAGGCTTTGGGCGTTCTGTGTCAATGCGTTGAGGCGCAGCAGTTGACTGGACATCTAGGGGTAAAGCACTGTTTCGGTGCGGGCCGCGAGAGCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGATCCGATTTCAAATGAAGATAAACTGTTCGACGAAATGTATAAGAGAGAAGAATTGATGAAATCGATTGCTGTTGTTGTACAAAGGGGAAGTGCAGAAGGGCGTTTTTACGCTGTGAAAATCCTTAGAAATATTGCTAAAAACACCAATTACAATTGGGGATCTATTATGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCGGACGAATTTCCCACAAAGGCTAGCTCATGTGCATTAGATGTAATGATAGAAATTCTCTCATCCTCGAAAAGGAGTCGAGTGAAGGCGATCGAAGCTGGGGCAGTGTGGATTCTGATCGAGCTTTTACCGGAGTCGGGGCGATCGAAATGCGAGAGAATGCTGCAGATTCTGCAGAGTTTATGTGAATGTGCTGAAGGGAGATTAGCTTTGGTAGAACATGGGATGGGAATTGCAGCTGTGACTAAGAAGATTTTGAATGTTTCATATGTAGCAACAAAAATTGGGGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCAAGGGAAAGGGTTGTAGATGAGATGATGGCATGTGGGGCAGTGAAGAAGCTGCTGATGATACTACACATGGACAGCACCGGTGTCGGTGACGGTGGCCGGAGTTCGACCAAGGAGAAGGTGATCAAGATTTTGAAGATGCATGGGAACAAATGGAGGCGTAGTCCATGTTTTCCTAGTGAGTTGAAGAATTATTTGAAGTTTGTGAATGATGAATGATATTGATCTTTGCTAAAGAAATCAACATACTATAGGAAATAATAATAATAATAATCCGTTTGTAACACATTTTTGCTCAATTTTAATTGAGTTTTGGAAATATATAGTAATTGAATAGGG

Coding sequence (CDS)

ATGGATTCTGATTTTCCTCCTCTTTTTCGATGCCCCATTTCCATGGAGCTCATGGAAGATCCGGTCACTGTCTCTACCGGCGTCACCTACGATAGAAAGAGCATTGAAAAATGGCTTTTTACTTACAATAAAAAAACTTGTCCGGCGACGATGCAGACACTCGCTGCCGGTGCTGAAGACTTCGTCATCACCCCTAATCTGAATCTCAAGCGCCTCATTCTCGCTTGGAAAATCAATGATTCTGCCTTGACGCCGGCGACGGCGCTCGAGGAAGTCTCGTCGCTTCTTGCTACTGTTGAGTCTTCTCCATTCAAGGTTAGTTCTTTGAGGAAATTGAGATCGATGGTTGAAATGAACGATGGGATGAAATCGGAGTTTATTAGATTGAGTGGGATTGGGATTGTTGTTAATGTTGTGATTCAAATTCTTATTGATTGTTCTGATTTCTCGACGTTGGACACTTGTGAAGAGGCTTTGGGCGTTCTGTGTCAATGCGTTGAGGCGCAGCAGTTGACTGGACATCTAGGGGTAAAGCACTGTTTCGGTGCGGGCCGCGAGAGCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGATCCGATTTCAAATGAAGATAAACTGTTCGACGAAATGTATAAGAGAGAAGAATTGATGAAATCGATTGCTGTTGTTGTACAAAGGGGAAGTGCAGAAGGGCGTTTTTACGCTGTGAAAATCCTTAGAAATATTGCTAAAAACACCAATTACAATTGGGGATCTATTATGGAAGAACAGGGGATTGATTTCTTCAAGCCATTGTTGGAGCTTGTATCGGACGAATTTCCCACAAAGGCTAGCTCATGTGCATTAGATGTAATGATAGAAATTCTCTCATCCTCGAAAAGGAGTCGAGTGAAGGCGATCGAAGCTGGGGCAGTGTGGATTCTGATCGAGCTTTTACCGGAGTCGGGGCGATCGAAATGCGAGAGAATGCTGCAGATTCTGCAGAGTTTATGTGAATGTGCTGAAGGGAGATTAGCTTTGGTAGAACATGGGATGGGAATTGCAGCTGTGACTAAGAAGATTTTGAATGTTTCATATGTAGCAACAAAAATTGGGGTGAAGATTTTGCTTTGGATTAGTAGTTTTCACCCAAGGGAAAGGGTTGTAGATGAGATGATGGCATGTGGGGCAGTGAAGAAGCTGCTGATGATACTACACATGGACAGCACCGGTGTCGGTGACGGTGGCCGGAGTTCGACCAAGGAGAAGGTGATCAAGATTTTGAAGATGCATGGGAACAAATGGAGGCGTAGTCCATGTTTTCCTAGTGAGTTGAAGAATTATTTGAAGTTTGTGAATGATGAATGA

Protein sequence

MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMNDGMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHCFGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE*

Homology

[Close]

BLAST of CSPI03G10010 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.6e-50
Identity = 144/455 (31.65%), Postives = 221/455 (48.57%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVI 63
           + PP F CPIS+E+M+DPV VSTG+TYDR SIEKWLF   K +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDIT----DADL 70

Query: 64  TPNLNLKRLILAW-KINDS-------ALTPATALEEVSSLLA-TVESSPFKVSSLRKLRS 123
           TPN  L+RLI +W  +N S          P     E+  L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 124 MVEMNDGMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGV---LCQCVEAQQL 183
           +V  N                              +T   C EA GV   L   V     
Sbjct: 131 IVSEN------------------------------ATNKRCLEAAGVPEFLANIVSNDS- 190

Query: 184 TGHLGVKHCFGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREE--LMKSIAVVVQRG 243
                         E+G+ + + LN  Y   ++E  L + +  +++  ++KS+  ++QRG
Sbjct: 191 --------------ENGSLTDEALNLLYHLETSETVLKNLLNNKKDNNIVKSLTKIMQRG 250

Query: 244 SAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIE 303
             E R YA  +L+NI +  +      ++    + F  +++++ D    KA+  A+ +++ 
Sbjct: 251 MYESRVYATLLLKNILEVADPMQSMTLKP---EVFTEVVQILDDRISQKATKAAMHILVN 310

Query: 304 ILSSSKRSRVKAIEAGAVWILIELLPE----SGRSKCERMLQILQSLCECAEGRLALVEH 363
           I     R+R KA+EAG + ++IELL +    S R   E  + +L  LC+CAEGR   + H
Sbjct: 311 ICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNH 370

Query: 364 GMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDST 423
           G  IA V KKIL VS  A+   V++LL +  F     ++ EM+  G V KL ++L +   
Sbjct: 371 GAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCG 405

Query: 424 GVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSEL 441
           G        TKEK  ++LK+H   W+ SPC P  +
Sbjct: 431 G-------KTKEKAKELLKLHARVWKDSPCLPKNM 405

[Close]

BLAST of CSPI03G10010 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.4e-48
Identity = 138/452 (30.53%), Postives = 229/452 (50.66%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVI 63
           + P  F CPIS+++M+DPV VSTG+TYDR+SIEKWLF+  K +CP T Q +     +  +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT----ETDL 65

Query: 64  TPNLNLKRLILAW-KINDS-------ALTPATALEEVSSLLATVESSPF-KVSSLRKLRS 123
           TPN  L+RLI +W  +N S          P     E+  L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 124 MVEMNDGMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGH 183
           +V  N   K   +  + +   +  ++   +D  +  +       L  +CQ   +  L   
Sbjct: 126 IVSENTTNK-RCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQ---SNMLENR 185

Query: 184 LGVKHCFGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKRE--ELMKSIAVVVQRGSAE 243
                       S +   + L+  Y   ++E  L   +  ++   L+K++  ++QRG  E
Sbjct: 186 FD---------SSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYE 245

Query: 244 GRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILS 303
            R YA  +L+ + +  +     ++E    + F  +++++ D+   KA+  A+ +++ I  
Sbjct: 246 SRAYAALLLKKLLEVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITC 305

Query: 304 SSKRSRVKAIEAGAVWILIELLPE----SGRSKCERMLQILQSLCECAEGRLALVEHGMG 363
              R+R KA+E G + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  
Sbjct: 306 PWGRNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAA 365

Query: 364 IAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVG 423
           IA V+KKIL VS + ++  V++LL +  F     ++ EM+  G V KL ++L + S G  
Sbjct: 366 IAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQV-SCG-- 425

Query: 424 DGGRSSTKEKVIKILKMHGNKWRRSPCFPSEL 441
               + TKEK  ++LK+H   WR SPC P  L
Sbjct: 426 ----NKTKEKAKELLKLHARVWRESPCVPRNL 429

[Close]

BLAST of CSPI03G10010 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.5e-32
Identity = 132/474 (27.85%), Postives = 211/474 (44.51%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVI 63
           + P  F CPIS+E+M+DPVT  +G+TYDR++I KWL      +CP T Q L   ++   +
Sbjct: 9   EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSD---L 68

Query: 64  TPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMNDGMK 123
           TPN  L+RLI  W + +               ++T    P K++ + +++++ +      
Sbjct: 69  TPNHMLRRLIQHWCVENETRGVVR--------ISTPRVPPGKLNVVEEIKNLKKFGQEAL 128

Query: 124 SEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHCF-- 183
                L  + ++                +D     L  +C+C   + L   L V  C   
Sbjct: 129 GREETLQKLEVL---------------AMDGNNRRL--MCECGVHKSLI--LFVVKCTSE 188

Query: 184 ---GAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAV 243
              G  R  G      L       SN+ K    + + + +M+S+  V+ +     + Y +
Sbjct: 189 DEDGRRRIKGLDESLRLLHLIGIPSNDAKTI--LMENDRVMESLTWVLHQEDFLSKAYTI 248

Query: 244 KILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSD-------EFPT-------------- 303
            +LRN+   T Y    I+E    + FK ++  + D         PT              
Sbjct: 249 VLLRNL---TEYTSSHIVERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLG 308

Query: 304 --------------KASSCALDVMIEILSSSKRSRVKAIEAGAVWILIEL-LPESGRSK- 363
                         +A + AL +++E  SS  R+R   ++ GAV  LIEL +  +G  + 
Sbjct: 309 KTEPSKLDHSLVIKQAVTAALMILLE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRI 368

Query: 364 CERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRE 423
            E ML +L  LC CA GR  ++ H  GIA VTK++L VS  A    + IL  +S F P  
Sbjct: 369 TELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPEN 428

Query: 424 RVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPC 436
            VV+EM+  G V+KL  +L MD          + KEK  +ILK H ++W++ PC
Sbjct: 429 MVVEEMVNVGTVEKLCSVLGMDC-------GLNLKEKAKEILKDHFDEWKKFPC 437

[Close]

BLAST of CSPI03G10010 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.0e-25
Identity = 123/441 (27.89%), Postives = 187/441 (42.40%), Query Frame = 0


Query: 6   PPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVITP 65
           P  FRCPIS+ELM+DPVTV TG TYDR SIE W+   N  TCP T   L+    DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS----DFTLIP 74

Query: 66  NLNLKRLILAWKINDSA---------------LTPATALEEVSSLLATVESSPFKVSSLR 125
           N  L+RLI  W + + +                +    L + S++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 126 KLRSMVEMNDGMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQ 185
           +LR     +D  +     L        ++I+IL   +  S L +   AL V+    E  Q
Sbjct: 135 RLRGFARDSDKNRV----LIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQ 194

Query: 186 LTGHLGVKHCFGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGS 245
                           S    R    TR         LFD   +      ++  +V  G+
Sbjct: 195 FVS------------ISSDPGRVEFLTRL--------LFDSSIETRVNAAALIEIVSTGT 254

Query: 246 AEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEI 305
                          K+ +   GSI   + +  F+ +L+L+ +   ++ +       +  
Sbjct: 255 ---------------KSADLK-GSISNSESV--FEGVLDLLRNPISSRRALKIGIKTLFA 314

Query: 306 LSSSKRSRVKAIEAGAVWILIE-LLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGI 365
           L S K +R  AI AGA  ILI+ L  +  R   ER L  ++ LC   EG  A  EH + +
Sbjct: 315 LCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTV 374

Query: 366 AAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGD 425
             + K IL VS  AT+     LL + +   R R  +E    G V +LL+++  + T    
Sbjct: 375 PLLVKTILRVSDRATEYAAGALLALCTAEERWR--EEAAGAGVVVQLLLMVQSECT---- 400

Query: 426 GGRSSTKEKVIKILKMHGNKW 431
                 K+K  K+LK+  + W
Sbjct: 435 ---ERAKKKAQKLLKLLRDSW 400

[Close]

BLAST of CSPI03G10010 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.4e-24
Identity = 116/447 (25.95%), Postives = 205/447 (45.86%), Query Frame = 0


Query: 6   PPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVITP 65
           PP F+CPIS++LM+DPV +STG+TYDR SIE W+ + N KTCP T   L      F   P
Sbjct: 32  PPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGN-KTCPVTNTVLTT----FDQIP 91

Query: 66  NLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSL-RKLRSMVEMNDGMKS 125
           N  ++++I  W +       +  ++ + +    V   P +V  + RKL S     D  K 
Sbjct: 92  NHTIRKMIQGWCVEKG----SPLIQRIPT--PRVPLMPCEVYEISRKLSSATRRGDYEK- 151

Query: 126 EFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALG-VLCQCVEA----QQLTGHLG--- 185
                   G+++   I+ L D S+ +     E ++G VLC C +     ++LT  L    
Sbjct: 152 -------CGVIIE-KIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKLTFMLNEIL 211

Query: 186 --VKHCFGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGR 245
             +   F  G E  +K     + R                       +A +++      R
Sbjct: 212 SLLTWMFPIGLEGISKLASATSFR----------------------CVAGLLKSTDDSVR 271

Query: 246 FYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSS 305
             A  I++ I         S   E G+   + L++L+ D   + ++  +L  + +++   
Sbjct: 272 QNAAFIMKEILSLDETRVHSFAVENGV--AEALVKLIRDSVSSSSTKSSLIAIYQMVLQK 331

Query: 306 KRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTK 365
                + +E G V I +E++ ++  S CE+ L +L ++CE   GR  + ++ + +  + K
Sbjct: 332 PEIASEFLEIGLVSITVEMIVDAENSVCEKALAVLDAICETEHGREEVRKNALVMPLLVK 391

Query: 366 KILNVSYVATKIGVKIL--LWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGR 425
           KI  VS +AT+  + ++  LW +        V++ +  GA +K+L++L +   G G+   
Sbjct: 392 KIAKVSELATRSSMSMILKLWKTG---NTVAVEDAVRLGAFQKVLLVLQV---GYGE--- 424

Query: 426 SSTKEKVIKILKMHGNKWR-RSPCFPS 439
             TKEK  ++LKM   + +  S C  S
Sbjct: 452 -ETKEKATELLKMMNTQMKLMSDCVDS 424

[Close]

BLAST of CSPI03G10010 vs. ExPASy TrEMBL
Match: A0A0A0L6J2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE=4 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.6e-226
Identity = 414/450 (92.00%), Postives = 414/450 (92.00%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND
Sbjct: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
           GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQ                
Sbjct: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQF--------------- 180

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI
Sbjct: 181 --------------------PISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK
Sbjct: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
           YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI
Sbjct: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 415

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of CSPI03G10010 vs. ExPASy TrEMBL
Match: A0A5D3D2G2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003490 PE=4 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.5e-221
Identity = 402/450 (89.33%), Postives = 408/450 (90.67%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGA+D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FVITPNL LKRLILAWKINDSA TPATALEEVSSLLATVESSPFKVSSLRKLRSM+EMND
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSMIEMND 120

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
           GMKSEF+RLSGIGIVVNVVIQILIDCSDFSTL+TCEEALGVLCQ                
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQF--------------- 180

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PISNEDKLFDEMYKREELMKSI VV+QRGSAEGRFYAVKI
Sbjct: 181 --------------------PISNEDKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKI 240

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWGS+MEEQ IDFFKPLLELVSDEFPTKASSCALDVMI ILSSSKRSRVK
Sbjct: 241 LRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEFPTKASSCALDVMIGILSSSKRSRVK 300

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
           YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDS GVGDGGRSSTKEKVI
Sbjct: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVI 415

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of CSPI03G10010 vs. ExPASy TrEMBL
Match: A0A1S3AWJ2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483401 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.3e-220
Identity = 401/450 (89.11%), Postives = 408/450 (90.67%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGA+D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FVITPNL LKRLILAWKINDSA TPATALEEVSSLLATVESSPFKVSSLRKLRS++EMND
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
           GMKSEF+RLSGIGIVVNVVIQILIDCSDFSTL+TCEEALGVLCQ                
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQF--------------- 180

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PISNEDKLFDEMYKREELMKSI VV+QRGSAEGRFYAVKI
Sbjct: 181 --------------------PISNEDKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKI 240

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWGS+MEEQ IDFFKPLLELVSDEFPTKASSCALDVMI ILSSSKRSRVK
Sbjct: 241 LRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEFPTKASSCALDVMIGILSSSKRSRVK 300

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
           YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDS GVGDGGRSSTKEKVI
Sbjct: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVI 415

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of CSPI03G10010 vs. ExPASy TrEMBL
Match: A0A6J1IYW3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 PE=4 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.9e-201
Identity = 363/450 (80.67%), Postives = 392/450 (87.11%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G  D
Sbjct: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FV+TPNL LKR+ILAWKIN+S+ +PATA+++V SLLATVESSPFKVSSLRKLRSM+EM D
Sbjct: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
            +KSEFIRL+GIGIVVNVV+QILIDCSDFSTL+ CEEALGVL Q                
Sbjct: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQF--------------- 192

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PISNED+LFDEM KREELMKS+A+V+QRGSAEGRFYAVKI
Sbjct: 193 --------------------PISNEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKI 252

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWG +MEEQGID FKPLLELVSDEFPTK+SSCALDVMIEIL+SSKRSRVK
Sbjct: 253 LRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVK 312

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPES RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 313 AIEAGAVWILIELLPESSRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 372

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
            VATKIGVKIL WISSFHP+ERVV+EMMACGAVKKLLMILHM  +GVG GGRSSTKEKVI
Sbjct: 373 NVATKIGVKILFWISSFHPKERVVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVI 427

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHG+ WRRSPCFP ELKNYLK VND+
Sbjct: 433 KILKMHGSSWRRSPCFPCELKNYLKLVNDD 427

[Close]

BLAST of CSPI03G10010 vs. ExPASy TrEMBL
Match: A0A6J1E9J6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432079 PE=4 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 3.3e-200
Identity = 363/450 (80.67%), Postives = 390/450 (86.67%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G  D
Sbjct: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FV+TPNL LKR+ILAWKIN+S+ +PATA+++V SLLATVESSPFKVSSLRKLRSM+EM D
Sbjct: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
            +KSEFIRL+GIGIVVNVV+QILIDCSDFSTL+ CEEALGVL Q                
Sbjct: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQF--------------- 192

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PISNEDKLFDEM KREE MKS+A+V+QRGSAEGRFYAVKI
Sbjct: 193 --------------------PISNEDKLFDEMSKREEWMKSVAIVLQRGSAEGRFYAVKI 252

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWG +MEEQGID FKPLLELVSDEFPTK+SSCALDVMIEIL+SSKRSRVK
Sbjct: 253 LRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVK 312

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPES RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 313 AIEAGAVWILIELLPESSRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 372

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
            VATKIGVKILLWISSFHP+ERVV+EMMACGAVKKLLMILHM S GVG GGRSSTKEKVI
Sbjct: 373 NVATKIGVKILLWISSFHPKERVVEEMMACGAVKKLLMILHMASGGVGGGGRSSTKEKVI 427

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHG+ WRR PCFP ELK YLK VND+
Sbjct: 433 KILKMHGSSWRRGPCFPCELKYYLKLVNDD 427

[Close]

BLAST of CSPI03G10010 vs. NCBI nr
Match: XP_004134357.1 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN56649.1 hypothetical protein Csa_010227 [Cucumis sativus])

HSP 1 Score: 795.0 bits (2052), Expect = 3.3e-226
Identity = 414/450 (92.00%), Postives = 414/450 (92.00%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND
Sbjct: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
           GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQ                
Sbjct: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQF--------------- 180

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI
Sbjct: 181 --------------------PISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK
Sbjct: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
           YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI
Sbjct: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 415

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of CSPI03G10010 vs. NCBI nr
Match: KAA0049079.1 (E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa] >TYK17484.1 E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa])

HSP 1 Score: 776.9 bits (2005), Expect = 9.2e-221
Identity = 402/450 (89.33%), Postives = 408/450 (90.67%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGA+D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FVITPNL LKRLILAWKINDSA TPATALEEVSSLLATVESSPFKVSSLRKLRSM+EMND
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSMIEMND 120

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
           GMKSEF+RLSGIGIVVNVVIQILIDCSDFSTL+TCEEALGVLCQ                
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQF--------------- 180

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PISNEDKLFDEMYKREELMKSI VV+QRGSAEGRFYAVKI
Sbjct: 181 --------------------PISNEDKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKI 240

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWGS+MEEQ IDFFKPLLELVSDEFPTKASSCALDVMI ILSSSKRSRVK
Sbjct: 241 LRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEFPTKASSCALDVMIGILSSSKRSRVK 300

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
           YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDS GVGDGGRSSTKEKVI
Sbjct: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVI 415

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of CSPI03G10010 vs. NCBI nr
Match: XP_008438233.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 775.4 bits (2001), Expect = 2.7e-220
Identity = 401/450 (89.11%), Postives = 408/450 (90.67%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGA+D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FVITPNL LKRLILAWKINDSA TPATALEEVSSLLATVESSPFKVSSLRKLRS++EMND
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
           GMKSEF+RLSGIGIVVNVVIQILIDCSDFSTL+TCEEALGVLCQ                
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQF--------------- 180

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PISNEDKLFDEMYKREELMKSI VV+QRGSAEGRFYAVKI
Sbjct: 181 --------------------PISNEDKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKI 240

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWGS+MEEQ IDFFKPLLELVSDEFPTKASSCALDVMI ILSSSKRSRVK
Sbjct: 241 LRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEFPTKASSCALDVMIGILSSSKRSRVK 300

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
           YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDS GVGDGGRSSTKEKVI
Sbjct: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVI 415

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of CSPI03G10010 vs. NCBI nr
Match: XP_038902365.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 748.0 bits (1930), Expect = 4.6e-212
Identity = 386/450 (85.78%), Postives = 403/450 (89.56%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           M+SDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTL+AG ED
Sbjct: 1   MESDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLSAGDED 60

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FVITPNL LKRLILAWKI DS+ +PATALEEV SLLATVESSPFKVSSLRKLRSM+EM+D
Sbjct: 61  FVITPNLTLKRLILAWKITDSSSSPATALEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
           GMKSEFIR +GIGIVVNVVIQILIDCSDFSTL+TCEEALGVLCQ                
Sbjct: 121 GMKSEFIRSNGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQF--------------- 180

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PIS+EDKLFDEM KREELMKS+A+V+QRGSAEGRFYAVKI
Sbjct: 181 --------------------PISDEDKLFDEMSKREELMKSVAIVLQRGSAEGRFYAVKI 240

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWGS+MEEQGIDFFKPLLELVSDEF TKASSCALDVMIEIL+SSKRSRVK
Sbjct: 241 LRNIAKNTNYNWGSVMEEQGIDFFKPLLELVSDEFATKASSCALDVMIEILASSKRSRVK 300

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
            VATK+GVKILLWISSFHPRERVV+EMMACGAVKKLLMILHMDS GVGDGGRSSTKEKVI
Sbjct: 361 NVATKMGVKILLWISSFHPRERVVEEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVI 415

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHGN WRRSPCFPSELKNYLKFVND+
Sbjct: 421 KILKMHGNSWRRSPCFPSELKNYLKFVNDD 415

[Close]

BLAST of CSPI03G10010 vs. NCBI nr
Match: XP_022980298.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 710.7 bits (1833), Expect = 8.1e-201
Identity = 363/450 (80.67%), Postives = 392/450 (87.11%), Query Frame = 0


Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G  D
Sbjct: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72

Query: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120
           FV+TPNL LKR+ILAWKIN+S+ +PATA+++V SLLATVESSPFKVSSLRKLRSM+EM D
Sbjct: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132

Query: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHC 180
            +KSEFIRL+GIGIVVNVV+QILIDCSDFSTL+ CEEALGVL Q                
Sbjct: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQF--------------- 192

Query: 181 FGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAVKI 240
                               PISNED+LFDEM KREELMKS+A+V+QRGSAEGRFYAVKI
Sbjct: 193 --------------------PISNEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKI 252

Query: 241 LRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSSKRSRVK 300
           LRNIAKNTNYNWG +MEEQGID FKPLLELVSDEFPTK+SSCALDVMIEIL+SSKRSRVK
Sbjct: 253 LRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVK 312

Query: 301 AIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 360
           AIEAGAVWILIELLPES RSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS
Sbjct: 313 AIEAGAVWILIELLPESSRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 372

Query: 361 YVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVI 420
            VATKIGVKIL WISSFHP+ERVV+EMMACGAVKKLLMILHM  +GVG GGRSSTKEKVI
Sbjct: 373 NVATKIGVKILFWISSFHPKERVVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVI 427

Query: 421 KILKMHGNKWRRSPCFPSELKNYLKFVNDE 451
           KILKMHG+ WRRSPCFP ELKNYLK VND+
Sbjct: 433 KILKMHGSSWRRSPCFPCELKNYLKLVNDD 427

[Close]

BLAST of CSPI03G10010 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-51
Identity = 144/455 (31.65%), Postives = 221/455 (48.57%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVI 63
           + PP F CPIS+E+M+DPV VSTG+TYDR SIEKWLF   K +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDIT----DADL 70

Query: 64  TPNLNLKRLILAW-KINDS-------ALTPATALEEVSSLLA-TVESSPFKVSSLRKLRS 123
           TPN  L+RLI +W  +N S          P     E+  L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 124 MVEMNDGMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGV---LCQCVEAQQL 183
           +V  N                              +T   C EA GV   L   V     
Sbjct: 131 IVSEN------------------------------ATNKRCLEAAGVPEFLANIVSNDS- 190

Query: 184 TGHLGVKHCFGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREE--LMKSIAVVVQRG 243
                         E+G+ + + LN  Y   ++E  L + +  +++  ++KS+  ++QRG
Sbjct: 191 --------------ENGSLTDEALNLLYHLETSETVLKNLLNNKKDNNIVKSLTKIMQRG 250

Query: 244 SAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIE 303
             E R YA  +L+NI +  +      ++    + F  +++++ D    KA+  A+ +++ 
Sbjct: 251 MYESRVYATLLLKNILEVADPMQSMTLKP---EVFTEVVQILDDRISQKATKAAMHILVN 310

Query: 304 ILSSSKRSRVKAIEAGAVWILIELLPE----SGRSKCERMLQILQSLCECAEGRLALVEH 363
           I     R+R KA+EAG + ++IELL +    S R   E  + +L  LC+CAEGR   + H
Sbjct: 311 ICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNH 370

Query: 364 GMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDST 423
           G  IA V KKIL VS  A+   V++LL +  F     ++ EM+  G V KL ++L +   
Sbjct: 371 GAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCG 405

Query: 424 GVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSEL 441
           G        TKEK  ++LK+H   W+ SPC P  +
Sbjct: 431 G-------KTKEKAKELLKLHARVWKDSPCLPKNM 405

[Close]

BLAST of CSPI03G10010 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 193.7 bits (491), Expect = 3.1e-49
Identity = 138/452 (30.53%), Postives = 229/452 (50.66%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVI 63
           + P  F CPIS+++M+DPV VSTG+TYDR+SIEKWLF+  K +CP T Q +     +  +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT----ETDL 65

Query: 64  TPNLNLKRLILAW-KINDS-------ALTPATALEEVSSLLATVESSPF-KVSSLRKLRS 123
           TPN  L+RLI +W  +N S          P     E+  L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 124 MVEMNDGMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGH 183
           +V  N   K   +  + +   +  ++   +D  +  +       L  +CQ   +  L   
Sbjct: 126 IVSENTTNK-RCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQ---SNMLENR 185

Query: 184 LGVKHCFGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKRE--ELMKSIAVVVQRGSAE 243
                       S +   + L+  Y   ++E  L   +  ++   L+K++  ++QRG  E
Sbjct: 186 FD---------SSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYE 245

Query: 244 GRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILS 303
            R YA  +L+ + +  +     ++E    + F  +++++ D+   KA+  A+ +++ I  
Sbjct: 246 SRAYAALLLKKLLEVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITC 305

Query: 304 SSKRSRVKAIEAGAVWILIELLPE----SGRSKCERMLQILQSLCECAEGRLALVEHGMG 363
              R+R KA+E G + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  
Sbjct: 306 PWGRNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAA 365

Query: 364 IAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVG 423
           IA V+KKIL VS + ++  V++LL +  F     ++ EM+  G V KL ++L + S G  
Sbjct: 366 IAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQV-SCG-- 425

Query: 424 DGGRSSTKEKVIKILKMHGNKWRRSPCFPSEL 441
               + TKEK  ++LK+H   WR SPC P  L
Sbjct: 426 ----NKTKEKAKELLKLHARVWRESPCVPRNL 429

[Close]

BLAST of CSPI03G10010 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-33
Identity = 132/474 (27.85%), Postives = 211/474 (44.51%), Query Frame = 0


Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVI 63
           + P  F CPIS+E+M+DPVT  +G+TYDR++I KWL      +CP T Q L   ++   +
Sbjct: 23  EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSD---L 82

Query: 64  TPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMNDGMK 123
           TPN  L+RLI  W + +               ++T    P K++ + +++++ +      
Sbjct: 83  TPNHMLRRLIQHWCVENETRGVVR--------ISTPRVPPGKLNVVEEIKNLKKFGQEAL 142

Query: 124 SEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQLTGHLGVKHCF-- 183
                L  + ++                +D     L  +C+C   + L   L V  C   
Sbjct: 143 GREETLQKLEVL---------------AMDGNNRRL--MCECGVHKSLI--LFVVKCTSE 202

Query: 184 ---GAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGRFYAV 243
              G  R  G      L       SN+ K    + + + +M+S+  V+ +     + Y +
Sbjct: 203 DEDGRRRIKGLDESLRLLHLIGIPSNDAKTI--LMENDRVMESLTWVLHQEDFLSKAYTI 262

Query: 244 KILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSD-------EFPT-------------- 303
            +LRN+   T Y    I+E    + FK ++  + D         PT              
Sbjct: 263 VLLRNL---TEYTSSHIVERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLG 322

Query: 304 --------------KASSCALDVMIEILSSSKRSRVKAIEAGAVWILIEL-LPESGRSK- 363
                         +A + AL +++E  SS  R+R   ++ GAV  LIEL +  +G  + 
Sbjct: 323 KTEPSKLDHSLVIKQAVTAALMILLE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRI 382

Query: 364 CERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRE 423
            E ML +L  LC CA GR  ++ H  GIA VTK++L VS  A    + IL  +S F P  
Sbjct: 383 TELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPEN 442

Query: 424 RVVDEMMACGAVKKLLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPC 436
            VV+EM+  G V+KL  +L MD          + KEK  +ILK H ++W++ PC
Sbjct: 443 MVVEEMVNVGTVEKLCSVLGMDC-------GLNLKEKAKEILKDHFDEWKKFPC 451

[Close]

BLAST of CSPI03G10010 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 117.5 bits (293), Expect = 2.8e-26
Identity = 123/441 (27.89%), Postives = 187/441 (42.40%), Query Frame = 0


Query: 6   PPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVITP 65
           P  FRCPIS+ELM+DPVTV TG TYDR SIE W+   N  TCP T   L+    DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS----DFTLIP 74

Query: 66  NLNLKRLILAWKINDSA---------------LTPATALEEVSSLLATVESSPFKVSSLR 125
           N  L+RLI  W + + +                +    L + S++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 126 KLRSMVEMNDGMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQCVEAQQ 185
           +LR     +D  +     L        ++I+IL   +  S L +   AL V+    E  Q
Sbjct: 135 RLRGFARDSDKNRV----LIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQ 194

Query: 186 LTGHLGVKHCFGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGS 245
                           S    R    TR         LFD   +      ++  +V  G+
Sbjct: 195 FVS------------ISSDPGRVEFLTRL--------LFDSSIETRVNAAALIEIVSTGT 254

Query: 246 AEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEI 305
                          K+ +   GSI   + +  F+ +L+L+ +   ++ +       +  
Sbjct: 255 ---------------KSADLK-GSISNSESV--FEGVLDLLRNPISSRRALKIGIKTLFA 314

Query: 306 LSSSKRSRVKAIEAGAVWILIE-LLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGI 365
           L S K +R  AI AGA  ILI+ L  +  R   ER L  ++ LC   EG  A  EH + +
Sbjct: 315 LCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTV 374

Query: 366 AAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGD 425
             + K IL VS  AT+     LL + +   R R  +E    G V +LL+++  + T    
Sbjct: 375 PLLVKTILRVSDRATEYAAGALLALCTAEERWR--EEAAGAGVVVQLLLMVQSECT---- 400

Query: 426 GGRSSTKEKVIKILKMHGNKW 431
                 K+K  K+LK+  + W
Sbjct: 435 ---ERAKKKAQKLLKLLRDSW 400

[Close]

BLAST of CSPI03G10010 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 2.4e-25
Identity = 116/447 (25.95%), Postives = 205/447 (45.86%), Query Frame = 0


Query: 6   PPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAEDFVITP 65
           PP F+CPIS++LM+DPV +STG+TYDR SIE W+ + N KTCP T   L      F   P
Sbjct: 32  PPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGN-KTCPVTNTVLTT----FDQIP 91

Query: 66  NLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSL-RKLRSMVEMNDGMKS 125
           N  ++++I  W +       +  ++ + +    V   P +V  + RKL S     D  K 
Sbjct: 92  NHTIRKMIQGWCVEKG----SPLIQRIPT--PRVPLMPCEVYEISRKLSSATRRGDYEK- 151

Query: 126 EFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALG-VLCQCVEA----QQLTGHLG--- 185
                   G+++   I+ L D S+ +     E ++G VLC C +     ++LT  L    
Sbjct: 152 -------CGVIIE-KIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKLTFMLNEIL 211

Query: 186 --VKHCFGAGRESGTKSRQTLNTRYDPISNEDKLFDEMYKREELMKSIAVVVQRGSAEGR 245
             +   F  G E  +K     + R                       +A +++      R
Sbjct: 212 SLLTWMFPIGLEGISKLASATSFR----------------------CVAGLLKSTDDSVR 271

Query: 246 FYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEFPTKASSCALDVMIEILSSS 305
             A  I++ I         S   E G+   + L++L+ D   + ++  +L  + +++   
Sbjct: 272 QNAAFIMKEILSLDETRVHSFAVENGV--AEALVKLIRDSVSSSSTKSSLIAIYQMVLQK 331

Query: 306 KRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTK 365
                + +E G V I +E++ ++  S CE+ L +L ++CE   GR  + ++ + +  + K
Sbjct: 332 PEIASEFLEIGLVSITVEMIVDAENSVCEKALAVLDAICETEHGREEVRKNALVMPLLVK 391

Query: 366 KILNVSYVATKIGVKIL--LWISSFHPRERVVDEMMACGAVKKLLMILHMDSTGVGDGGR 425
           KI  VS +AT+  + ++  LW +        V++ +  GA +K+L++L +   G G+   
Sbjct: 392 KIAKVSELATRSSMSMILKLWKTG---NTVAVEDAVRLGAFQKVLLVLQV---GYGE--- 424

Query: 426 SSTKEKVIKILKMHGNKWR-RSPCFPS 439
             TKEK  ++LKM   + +  S C  S
Sbjct: 452 -ETKEKATELLKMMNTQMKLMSDCVDS 424

The following BLAST results are available for this feature:

BLAST of CSPI03G10010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TG3	1.6e-50	31.65	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SVC6	4.4e-48	30.53	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q9SF15	1.5e-32	27.85	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	4.0e-25	27.89	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q5PNY6	3.4e-24	25.95	U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2...	[more]

BLAST of CSPI03G10010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6J2	1.6e-226	92.00	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE...	[more]
A0A5D3D2G2	4.5e-221	89.33	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3AWJ2	1.3e-220	89.11	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483401 PE=4 ...	[more]
A0A6J1IYW3	3.9e-201	80.67	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 P...	[more]
A0A6J1E9J6	3.3e-200	80.67	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432079...	[more]

BLAST of CSPI03G10010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134357.1	3.3e-226	92.00	E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN56649.1 hypothetical pro...	[more]
KAA0049079.1	9.2e-221	89.33	E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa] >TYK17484.1 E3...	[more]
XP_008438233.1	2.7e-220	89.11	PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo]	[more]
XP_038902365.1	4.6e-212	85.78	E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida]	[more]
XP_022980298.1	8.1e-201	80.67	E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima]	[more]

BLAST of CSPI03G10010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	1.1e-51	31.65	plant U-box 23	[more]
AT3G52450.1	3.1e-49	30.53	plant U-box 22	[more]
AT3G11840.1	1.1e-33	27.85	plant U-box 24	[more]
AT3G19380.1	2.8e-26	27.89	plant U-box 25	[more]
AT5G37490.1	2.4e-25	25.95	ARM repeat superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 8..76 e-value: 4.7E-20 score: 82.6
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 6..77 e-value: 5.1E-15 score: 55.4
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 4..83 score: 17.445486
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..87 e-value: 1.5E-21 score: 78.1
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 202..424 e-value: 4.8E-12 score: 47.1
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 5..167
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 210..448
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 210..448
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 5..167
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 9..52 e-value: 1.65974E-21 score: 84.9778
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 3..79
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 80..406

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G10010.1	CSPI03G10010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity