CSPI03G09900 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G09900
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description3'-5' ssDNA/RNA exonuclease TatD
LocationChr3: 8001329 .. 8005533 (-)
RNA-Seq ExpressionCSPI03G09900
SyntenyCSPI03G09900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTATTTGTTATTTTGTTATTCAGGTGAAGTGCGCGGCAATAGGTTTTGCGCGCGCGCTCTTCACAGCCGGAAGGCGGTTTCTGAGACGAAGTTCTGCTTCCATCGCTCGACGAGGTATCTCCGGCTGTCGATGTCGTCGTGAAGTTTATTGCAATTCGTTTTTTCGCGGTTTTTGTTTGAGTTTTATTCTCTACAGTTTATTTTATCGGACGCTTGAATTGGATCTCTACTTTGACGTATTCTTACTGTTTCGAAATTCATAGCGTTTCCGATTTGTTTCTATTTCGCTGCGTTCTTCGGACTGTCGCGATTTGAAGACAAGGTACTGTGTTGGCTTTGTGATTACTCTCTCTCGCTCTCTTGTTCTCCTGTTTCTTAAATGTCCAGCAGTAAATGAAACCGTAGGTTATCTCTGATTAGAAACTACTCGCACGTACATTCTCGATATGATTAATTGCCTGATGATGAATGGAACTGAAATAGATATATAATAGGTTTTTGAGTCGAAATCCTTCGCAGTTTGATAGCTAAGGTACTGTGTTCGCAATGCGATTTTTCATGTCTCTCTCGCTCGCTGAAATACGCCAATTTCTTTAATCTTCTGCGGTAGCTATTGTATTAGGTTTTCTTAAATCTCTTGTTCTCATGTTACTTAATCTCCGCAGTAAATGAAGTCGTAGGTTTTCTCTGATTAGAAAATACTCGCACGTACTTTCTCGACATGATAAATTACTTGATGATGAATTGAACTGAAATAGATATATAATAGGTTTTTGAGTTGAAATCCTTCGCAGTTTGATAGCTAAGGTACTGTATTTGCAGTGCGATTTTTCATATGTCTCTCTCGCTCGCTGAAATTCGCCATTTTCTTTAATCTTCTGCGGTAGCTATTGTATTAGGTTTTCTCGAGAAGGAAATGTTCTTACGCACTGTCTCAATGTGGTGAATTACATTATGAAATATTAAATGTATATGGATATAGGCTTTTAAATTTTAAGTTAATCATCCATAAACGGAAATTGACGGTACTGCTCTAGCTGTGCAATTTACTTGCGTTTTCTCAATCGTTGAGCTCAATTTTTTTAAGCTTCTACTGTCATTGAACTGTAATTGAAGCTGTAGGTTTTCTCCGAGCAGGAAATATTCTTATGCATTTTCTTGAAAAGATTAATTACATTTTGAAGAATTTTTAAGTTGAATTTTTTTTGGTGACATAAGAAGAAACTCTTTGCCTTGGATCTGTTTTCCTGACACGAGAAGAAACTCTCCTTTCCTTTTATACGCAGGAATGGCTAAATTGTCTTCTACGTTGAGACTATTTGATGCACATTGTCACCTCCAAGATCCAAGAATATTTGATAAAGCACCTCAATTAATTAGTGCAGCAGTGGATTCTGGTGTTGTTCGTTTTGCTGTTAATGGAATCTCTGAGGTACATTAGTTATGGATCTTGTGTTGTATATATATATTTTTTTATATGTGCCGTCTCCTGCTCATCTTTTTCATCTGTTGAATTTATAGAAAGATTGGCATTTGGTCAAGCAATTGAGTGAATTGCATCCTTGCATAATTCCATCCTTCGGACTTCATCCCTGGTAAGCACATACAAATTTTTTTTCTGCCATCATGAAGTATACTGTATGACATTCACCCAGCTTGCTGTATTTAGGTATGTTCCTGGAAGAACTCCTGGCTGGCTTGAAAAACTTAAGGAGTTTTTTGAAGCAGTACCAAGCGCAGCAGTTGGAGAGGTTGGTGTTTGTAGCCAAATCTAGATCCTTGAAGACCAATTCGTGCTTTGGATGAAAAAAAAAAAAAACAGATGAAATCAATCAGATGAATTTTTTCTGCTAAATAATTATTTTGTTCTTCTAGTGATATTCCTGAATCATCTTAAGAGCATTTTAGGGATGGTTTCTGAAGTTTTTAATACATGTTACTAGTGCCTTCTGGTTATTGACCTTCGATATTATTGTGCATACAAGAATTTTTATGAAAAGAGCTTCCTAGATTATGTGCCCTTAATTTTGTTATCTTTTTTCTTTAACTATGTGATACGCTACAGAATAGCACAAAGGTCTCTAGTGATTAATATGTTGGCTCAACATAAGATATTTTTTATTAGGTTGTAAATTTAGGACATGTTTCTTTTCAGATTGGTCTGGACAAAGGTTCACGAGGAAGGGAGATCAATTTTACGGATCAGGTATATGGCTATTATACATTTTCTTATGAGGTTATGAAAGAAATGTATGGCTATTATTCGTTTTCAGATTGTAATAAGGAAATTTTCTTGAACATTTCAGTGATTTAGACTGTTATTGACATAAAAAAATGCTGCAGATTGAGGTCTTTCAGCAACAGCTAGAGCTTGCTAAGTCTTTAGAAAAACCAGCATCCATCCACTGTGTTCGTGCTTTTGGTGACCTCCTTAATATAATGAAGTATGACTTTTCCTAATAACTATGTGTTTTTTTTTTGTTAATTTCAAATTGCTATTCTTAGTCATATTCCCTAATTAAATGCCTTGTTCTGTCATTGCAATTGCTTCAGATCTATGGGACCTTTTCCTGCTGGTTTCATTCTGCATTCATATCTAGGATCTGCTGAGATGGTTCCGGAGTTCTCCAAGCTAGGTGCTTACTTTTCATTCTCTGGTTTTCTAATGTCCATGGAGGCAAAGAAGGCAAAGAAAATGTTGAAAATGGTCAGTTCACTTTCTCTATTGTTTCTTATCTTTTGGAAAATTTTCGTGTGTGTGTACAAAAGATAAGATACTTGTATACGGAACAAGAACATGCGAGTAAAAAAGAGGGTAGATTGGAATATTCATCATAAAACCACTTGGTCAATAAAATTTCATCGGGTTACTAAAATGTTGTAAAAACTTCTATCTAGGGTGAACCATTTGAAAGAATGAAACACACCAAATCCTTTATAACTGTAACATCATAATTCTTGAATTTTTCCAGGTGCCTTCAGATAGGATTTTGTTGGAGACTGATGCGCCCGATGCACTTCCCAAGTCAAAGTCAAACTCCCTCTATTTAGTTGAAGGCGATCCTTCTCTTACCGAATTTCTTAAGGCCCAAGAACAATCTGCTCCAACAGGCTCACAACAAAGTACTTTGCTTTGTTCCTCTACAGATGCATCAACACTGCCGAGAGAGACTCTCAATCACCCAGCAAATATTCAAAATGTACTTCACTGAATCTTTCATTATAGCTGGTTAATTGATGTTTATTTTCGTATCGTTAAAATTGGAAGAGGCTTAAAGGATCATTTTTAAAAAAAAGCTTAAAGAAAACCAGCATAATAGCTCTCTGCACTGATAGCATTTTATAATCTGGAATTCAGGTTTTAATACTAGTAAAACTTATAGGTAAAAAGTTTCCTACTCTTCAGAAAAAAATGGCCATTTTCCTCTTCATTTAATCAAATTACACGGGGGCCTTGTTTGATATCTTTAATTAAAAAAAGTCCAAAGCTCCACTCTTGACCTGGTTCATTTTCCATTATCTTTTCTTAGCATATTACACGCTTTATTCTTCTTTACAGGTTCTTGATTACGTCGCATCTTTATTGGGGATCAGTAAAGAAGAACTTGCTGAATTGAGTTATAGAAATGCGGAGCATTTATTCTCTTTCAATGGTGTACAAACTTCTTAGGGAAGAACCCAAATATTTAGGTGATGTTTTTAACAACTTTGTATAATACCCCCTTCTCACTAACATTTATCTTACCATCTAGCAATGAACTCTTTTTTTGGGAATAAAAGTCAGGCGATGCCTTTCTGGTGCTTGTCTTTGGTATCGTGTATCAATGAAAAGAAGAACCTTCTAACAACGCAGCCATTGTAGGACTAATGGCATTTGGGAGGTAGATATATGTATTAACAAGAATGGTAGACCACTTACCTAATCTTTATGCACAGTTACTTCAAATGTATGGATGATATAGATGACTAACAGCAGGCAAGTTGATAATTGATACAATGGGCATTGTGGGGATGCATGGAATTTCAGAGGTAGATGTGCTAACCTGGTCAATAAGACTTAGATCACTAACCTAGTGTTTGTGCATTCATATTATTTTTTACTATATAAAGAAAGATGATTATTAGTAGGAACATTGCATTTATGCTGCCACAAACGGTTTAATCTGGAGCTGTAAGGTCAAAAGATGAACTAGGAGATGAGCAG

mRNA sequence

GTTATTTGTTATTTTGTTATTCAGGTGAAGTGCGCGGCAATAGGTTTTGCGCGCGCGCTCTTCACAGCCGGAAGGCGGTTTCTGAGACGAAGTTCTGCTTCCATCGCTCGACGAGGTATCTCCGGCTGTCGATGTCGTCGTGAAGTTTATTGCAATTCGTTTTTTCGCGGTTTTTGTTTGAGTTTTATTCTCTACAGTTTATTTTATCGGACGCTTGAATTGGATCTCTACTTTGACGTATTCTTACTGTTTCGAAATTCATAGCGTTTCCGATTTGTTTCTATTTCGCTGCGTTCTTCGGACTGTCGCGATTTGAAGACAAGGAATGGCTAAATTGTCTTCTACGTTGAGACTATTTGATGCACATTGTCACCTCCAAGATCCAAGAATATTTGATAAAGCACCTCAATTAATTAGTGCAGCAGTGGATTCTGGTGTTGTTCGTTTTGCTGTTAATGGAATCTCTGAGAAAGATTGGCATTTGGTCAAGCAATTGAGTGAATTGCATCCTTGCATAATTCCATCCTTCGGACTTCATCCCTGGTATGTTCCTGGAAGAACTCCTGGCTGGCTTGAAAAACTTAAGGAGTTTTTTGAAGCAGTACCAAGCGCAGCAGTTGGAGAGATTGGTCTGGACAAAGGTTCACGAGGAAGGGAGATCAATTTTACGGATCAGATTGAGGTCTTTCAGCAACAGCTAGAGCTTGCTAAGTCTTTAGAAAAACCAGCATCCATCCACTGTGTTCGTGCTTTTGGTGACCTCCTTAATATAATGAAATCTATGGGACCTTTTCCTGCTGGTTTCATTCTGCATTCATATCTAGGATCTGCTGAGATGGTTCCGGAGTTCTCCAAGCTAGGTGCTTACTTTTCATTCTCTGGTTTTCTAATGTCCATGGAGGCAAAGAAGGCAAAGAAAATGTTGAAAATGGTGCCTTCAGATAGGATTTTGTTGGAGACTGATGCGCCCGATGCACTTCCCAAGTCAAAGTCAAACTCCCTCTATTTAGTTGAAGGCGATCCTTCTCTTACCGAATTTCTTAAGGCCCAAGAACAATCTGCTCCAACAGGCTCACAACAAAGTACTTTGCTTTGTTCCTCTACAGATGCATCAACACTGCCGAGAGAGACTCTCAATCACCCAGCAAATATTCAAAATGTTCTTGATTACGTCGCATCTTTATTGGGGATCAGTAAAGAAGAACTTGCTGAATTGAGTTATAGAAATGCGGAGCATTTATTCTCTTTCAATGGTGTACAAACTTCTTAGGGAAGAACCCAAATATTTAGGTGATGTTTTTAACAACTTTGTATAATACCCCCTTCTCACTAACATTTATCTTACCATCTAGCAATGAACTCTTTTTTTGGGAATAAAAGTCAGGCGATGCCTTTCTGGTGCTTGTCTTTGGTATCGTGTATCAATGAAAAGAAGAACCTTCTAACAACGCAGCCATTGTAGGACTAATGGCATTTGGGAGGTAGATATATGTATTAACAAGAATGGTAGACCACTTACCTAATCTTTATGCACAGTTACTTCAAATGTATGGATGATATAGATGACTAACAGCAGGCAAGTTGATAATTGATACAATGGGCATTGTGGGGATGCATGGAATTTCAGAGGTAGATGTGCTAACCTGGTCAATAAGACTTAGATCACTAACCTAGTGTTTGTGCATTCATATTATTTTTTACTATATAAAGAAAGATGATTATTAGTAGGAACATTGCATTTATGCTGCCACAAACGGTTTAATCTGGAGCTGTAAGGTCAAAAGATGAACTAGGAGATGAGCAG

Coding sequence (CDS)

ATGGCTAAATTGTCTTCTACGTTGAGACTATTTGATGCACATTGTCACCTCCAAGATCCAAGAATATTTGATAAAGCACCTCAATTAATTAGTGCAGCAGTGGATTCTGGTGTTGTTCGTTTTGCTGTTAATGGAATCTCTGAGAAAGATTGGCATTTGGTCAAGCAATTGAGTGAATTGCATCCTTGCATAATTCCATCCTTCGGACTTCATCCCTGGTATGTTCCTGGAAGAACTCCTGGCTGGCTTGAAAAACTTAAGGAGTTTTTTGAAGCAGTACCAAGCGCAGCAGTTGGAGAGATTGGTCTGGACAAAGGTTCACGAGGAAGGGAGATCAATTTTACGGATCAGATTGAGGTCTTTCAGCAACAGCTAGAGCTTGCTAAGTCTTTAGAAAAACCAGCATCCATCCACTGTGTTCGTGCTTTTGGTGACCTCCTTAATATAATGAAATCTATGGGACCTTTTCCTGCTGGTTTCATTCTGCATTCATATCTAGGATCTGCTGAGATGGTTCCGGAGTTCTCCAAGCTAGGTGCTTACTTTTCATTCTCTGGTTTTCTAATGTCCATGGAGGCAAAGAAGGCAAAGAAAATGTTGAAAATGGTGCCTTCAGATAGGATTTTGTTGGAGACTGATGCGCCCGATGCACTTCCCAAGTCAAAGTCAAACTCCCTCTATTTAGTTGAAGGCGATCCTTCTCTTACCGAATTTCTTAAGGCCCAAGAACAATCTGCTCCAACAGGCTCACAACAAAGTACTTTGCTTTGTTCCTCTACAGATGCATCAACACTGCCGAGAGAGACTCTCAATCACCCAGCAAATATTCAAAATGTTCTTGATTACGTCGCATCTTTATTGGGGATCAGTAAAGAAGAACTTGCTGAATTGAGTTATAGAAATGCGGAGCATTTATTCTCTTTCAATGGTGTACAAACTTCTTAG

Protein sequence

MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEVFQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGAYFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLKAQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYRNAEHLFSFNGVQTS*
Homology
BLAST of CSPI03G09900 vs. ExPASy Swiss-Prot
Match: P37545 (Uncharacterized metal-dependent hydrolase YabD OS=Bacillus subtilis (strain 168) OX=224308 GN=yabD PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.0e-23
Identity = 85/302 (28.15%), Postives = 126/302 (41.72%), Query Frame = 0

Query: 10  LFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIIPSFG 69
           LFD H HL   +      ++I  A    V R  V G          ++ E +  I  + G
Sbjct: 2   LFDTHAHLNAEQYDTDLEEVIERAKAEKVERIVVVGFDRPTITRAMEMIEEYDFIYAAIG 61

Query: 70  LHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDK--GSRGREINFTDQIEVFQQQLEL 129
            HP      T   L  +KE        A+GE+GLD       ++I    Q EVF+ Q+ L
Sbjct: 62  WHPVDAIDMTEEDLAWIKELSAHEKVVAIGEMGLDYHWDKSPKDI----QKEVFRNQIAL 121

Query: 130 AKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGAYFSFSGF 189
           AK +  P  IH   A  D++ I+K  G    G I+H + GSAE+  E  K+  Y SF G 
Sbjct: 122 AKEVNLPIIIHNRDATEDVVTILKEEGAEAVGGIMHCFTGSAEVARECMKMNFYLSFGGP 181

Query: 190 LMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLKAQEQSAP 249
           +    AKK K+++K +P+DR+L+ETD P   P                            
Sbjct: 182 VTFKNAKKPKEVVKEIPNDRLLIETDCPFLTPHPF------------------------- 241

Query: 250 TGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYRNAEHLFS 309
                              R   N P+ ++ V + +A L  ++ EE+A ++  NA+ LF 
Sbjct: 242 -------------------RGKRNEPSYVKYVAEQIAELKEMTFEEIASITTENAKRLFR 255

BLAST of CSPI03G09900 vs. ExPASy Swiss-Prot
Match: Q05AV0 (Putative deoxyribonuclease tatdn3-A OS=Xenopus laevis OX=8355 GN=tatdn3-a PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 5.1e-19
Identity = 84/311 (27.01%), Postives = 136/311 (43.73%), Query Frame = 0

Query: 12  DAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVK--QLSELHP-CIIPSF 71
           D HCH+          ++I  + ++G+   A+  ++E      K   LS+ +P  I+P F
Sbjct: 7   DCHCHMTAEEFSQDTEEVIEESKENGIQ--ALISVTEHGGEFEKLIYLSDRYPDYIMPCF 66

Query: 72  GLHPWYVPGR-----TPGWLEKLKEFFEAVPSA--AVGEIGLD------KGSRGREINFT 131
           G+HP    G      T   LE +   F+    +  A+GEIGLD        S  RE    
Sbjct: 67  GIHPIQSNGDGQRSVTIKDLEPVLAQFDKYKDSLVAIGEIGLDFTPWLAPTSEQRE---- 126

Query: 132 DQIEVFQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEF 191
           +Q++VFQ QLE+AK ++ P ++H   A    ++ +K  G      +LH++ G      E 
Sbjct: 127 EQMKVFQMQLEMAKRMDLPVNVHSRSAGRQTVSFLKEQG--AEKVLLHNFAGRPSAAQEG 186

Query: 192 SKLGAYFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSL 251
            + G +FSF   ++  E +  KK++KM+P + I LETD+P   PK               
Sbjct: 187 VQAGYFFSFPPAVIRNEQR--KKLIKMIPLENICLETDSPSLGPK--------------- 246

Query: 252 TEFLKAQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELA 307
                                          +E  N P+NI+   +Y+AS+ G+  E + 
Sbjct: 247 -------------------------------KEDRNVPSNIRLSCEYIASVKGLMPERVR 261

BLAST of CSPI03G09900 vs. ExPASy Swiss-Prot
Match: Q8K9J1 (Uncharacterized metal-dependent hydrolase BUsg_343 OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) OX=198804 GN=BUsg_343 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.1e-18
Identity = 65/233 (27.90%), Postives = 111/233 (47.64%), Query Frame = 0

Query: 10  LFDAHCHL--------QDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSELH 69
           L D+HCH+         D  +  +  ++++ A ++ V +F     S ++++  K+L   +
Sbjct: 3   LIDSHCHIDQMNIDQKNDDLLHQEIEKILNKAYENHVKKFLTVSTSIQNFYKTKKLLSTY 62

Query: 70  PCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEVF 129
             I  S G+HP Y   +    L +LK+        A+GE GLD        N   Q + F
Sbjct: 63  DSIFYSCGIHPLYCQ-KEIECLNELKKLSYDKCVIALGETGLDYYYSFETKNL--QEKFF 122

Query: 130 QQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGAY 189
           ++ + +A +L KP  IH   A  D + I+K       G ILHS+  S +   E   +G Y
Sbjct: 123 REHIRIAITLNKPIIIHSRNAIKDTIKILKEENAEKCGGILHSFTESEKSAFELLDMGFY 182

Query: 190 FSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALP----KSKSNSLYLVE 231
            SFSG +   ++ +    LK +P +++L+ETD+P   P      K+   YL++
Sbjct: 183 ISFSGIITFKKSIELCNTLKKIPLEKLLIETDSPYLAPIPYRGKKNQPAYLLD 232

BLAST of CSPI03G09900 vs. ExPASy Swiss-Prot
Match: P73335 (D-aminoacyl-tRNA deacylase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=dtd3 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.6e-18
Identity = 76/302 (25.17%), Postives = 126/302 (41.72%), Query Frame = 0

Query: 8   LRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIIPS 67
           + L D H H+          QL      +GVV+   + +  +++  ++ L++  P +  +
Sbjct: 1   MHLVDTHVHINFDVFAADLDQLQHRWRQAGVVQLVHSCVKPQEFDQIQSLADRFPELFFA 60

Query: 68  FGLHPWYVPGRTPGWLEKLKEFFEAVPS-AAVGEIGLDKGSRGREINFTDQIEVFQQQLE 127
            GLHP            ++  + +A     A+GE+GLD     +  N   QIEVF+ QL 
Sbjct: 61  VGLHPLDAEDWQDNTAGQILAYAKADDRVVAIGEMGLD---FFKADNRDHQIEVFRAQLA 120

Query: 128 LAKSLEKPASIHCVRAFGDLLNIMKSM--GPFPAGFILHSYLGSAEMVPEFSKLGAYFSF 187
           +A+ L KP  IHC  A   +  ++        P   ++H + G+ E    F  LG Y SF
Sbjct: 121 IARELNKPVIIHCRDAAQTMRQVLTDFQAESGPVAGVMHCWGGTPEETQWFLDLGFYISF 180

Query: 188 SGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLKAQEQ 247
           SG +   +A+  +   +MVP DR+L+ETD P   P  +                      
Sbjct: 181 SGTVTFKKAEGIQASAQMVPPDRLLVETDCPFLAPVPQ---------------------- 240

Query: 248 SAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYRNAEH 307
                                 R   N PA +++V + +A+L  +  E LA+ +  NA +
Sbjct: 241 ----------------------RGKRNEPAFVRHVAEAIAALRHVPLETLAQQTTTNARN 255

BLAST of CSPI03G09900 vs. ExPASy Swiss-Prot
Match: E6WHK1 (3'-5' ssDNA/RNA exonuclease TatD OS=Pantoea sp. (strain At-9b) OX=592316 GN=tatD PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.6e-17
Identity = 58/214 (27.10%), Postives = 102/214 (47.66%), Query Frame = 0

Query: 10  LFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSELHPCII-PSF 69
           +FD   +L   +      Q+++ A D+GV    + G +  +    ++L+E HP     + 
Sbjct: 1   MFDIGVNLTSTQFAKDREQVVARARDAGVTGLLITGTNALESQQAQRLAEWHPGYCWSTA 60

Query: 70  GLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIE-VFQQQLEL 129
           G+HP +    +      L+   E+    A+GE GLD     R  +  DQ E  F  QL+L
Sbjct: 61  GVHPHHASAWSAETANTLRRLAESEQVVAIGECGLD---FNRNFSAHDQQEYAFDAQLQL 120

Query: 130 AKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGAYFSFSGF 189
           A  L+ P  +HC  A      I++   P   G + H + G+ E +     +G     +G+
Sbjct: 121 AAELQLPVFLHCREAHDRFAAILQPWLPKLVGAVAHCFTGTREELEACLAMGLSIGITGW 180

Query: 190 LM-SMEAKKAKKMLKMVPSDRILLETDAPDALPK 221
           +       + +++L ++P++R+LLETDAP  LP+
Sbjct: 181 VCDERRGMELRELLPLIPAERLLLETDAPWLLPR 211

BLAST of CSPI03G09900 vs. ExPASy TrEMBL
Match: A0A0A0L4G2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G127010 PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.4e-176
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL
Sbjct: 45  MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 104

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           HPCI+PSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV
Sbjct: 105 HPCIVPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 164

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA
Sbjct: 165 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 224

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK
Sbjct: 225 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 284

Query: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300
           AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR
Sbjct: 285 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 344

Query: 301 NAEHLFSFNGVQTS 315
           NAEHLFSFNGVQTS
Sbjct: 345 NAEHLFSFNGVQTS 358

BLAST of CSPI03G09900 vs. ExPASy TrEMBL
Match: A0A1S3AWG8 (uncharacterized metal-dependent hydrolase YabD isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483391 PE=4 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.7e-171
Identity = 305/314 (97.13%), Postives = 307/314 (97.77%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           MAKLSSTLRLFDAHCHLQDPRIFDKAPQLIS AVDSGVVRFAVNG SEKDWHLVKQLSEL
Sbjct: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISVAVDSGVVRFAVNGTSEKDWHLVKQLSEL 60

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           HPCI+PSFGLHPWYVPGRTP WLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV
Sbjct: 61  HPCIVPSFGLHPWYVPGRTPVWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLEKPASIHCVRAFGDLL IMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLKIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSMEAKKAKKMLK VPSDRILLETDAPDALPKSKSNSLYLVEGDP+LTEFLK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKTVPSDRILLETDAPDALPKSKSNSLYLVEGDPALTEFLK 240

Query: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300
           AQEQSAPTGSQQSTLLCSS DASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR
Sbjct: 241 AQEQSAPTGSQQSTLLCSSADASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300

Query: 301 NAEHLFSFNGVQTS 315
           NAEHLFSF GVQTS
Sbjct: 301 NAEHLFSFKGVQTS 314

BLAST of CSPI03G09900 vs. ExPASy TrEMBL
Match: A0A5A7U6E3 (Putative metal-dependent hydrolase YabD isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003580 PE=4 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 2.8e-169
Identity = 301/310 (97.10%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           MAKLSSTLRLFDAHCHLQDPRIFDKAPQLIS AVDSGVVRFAVNG SEKDWHLVKQLSEL
Sbjct: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISVAVDSGVVRFAVNGTSEKDWHLVKQLSEL 60

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           HPCI+PSFGLHPWYVPGRTP WLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV
Sbjct: 61  HPCIVPSFGLHPWYVPGRTPVWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLEKPASIHCVRAFGDLL IMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLKIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSMEAKKAKKMLK VPSDRILLETDAPDALPKSKSNSLYLVEGDP+LTEFLK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKTVPSDRILLETDAPDALPKSKSNSLYLVEGDPALTEFLK 240

Query: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300
           AQEQSAPTGSQQSTLLCSS DASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR
Sbjct: 241 AQEQSAPTGSQQSTLLCSSADASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300

Query: 301 NAEHLFSFNG 311
           NAEHLFSF G
Sbjct: 301 NAEHLFSFKG 310

BLAST of CSPI03G09900 vs. ExPASy TrEMBL
Match: A0A6J1CYM1 (uncharacterized protein LOC111015984 OS=Momordica charantia OX=3673 GN=LOC111015984 PE=4 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 2.9e-158
Identity = 285/314 (90.76%), Postives = 297/314 (94.59%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           MAKLS+TLRLFDAHCHLQDPRIFDKAPQLIS AVDSGVVRFAVNGISEKDWHLVKQLSEL
Sbjct: 1   MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 60

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           HPCI+PSFGLHPWYVP RTPGWLEKLKEFFEAVP+AAVGEIGLDKGSRGREI+FTDQ+EV
Sbjct: 61  HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 120

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLEKPASIHCVRAFGDLL IMKSMGPFPAGFILHSYLGSA+MVPEFSKLG 
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 180

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSM+AKKAKKMLKMVPSDRILLETDAPDALPK K +S+Y VEGDPSL E LK
Sbjct: 181 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 240

Query: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300
             EQSA TGS+QST L SSTDASTLP+ETLNHPANIQNVL YVASLL ISKEELAELSYR
Sbjct: 241 TGEQSASTGSEQST-LPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYR 300

Query: 301 NAEHLFSFNGVQTS 315
           NAEHLFSF G+QTS
Sbjct: 301 NAEHLFSFKGLQTS 313

BLAST of CSPI03G09900 vs. ExPASy TrEMBL
Match: A0A6J1HP04 (uncharacterized protein LOC111464387 OS=Cucurbita moschata OX=3662 GN=LOC111464387 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.0e-155
Identity = 282/315 (89.52%), Postives = 295/315 (93.65%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           M+KLS+TLRLFDAHCHLQDPRIFDKAPQLIS AVDSGV+RFAVNGISEKDWHLVKQLSE 
Sbjct: 1   MSKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVLRFAVNGISEKDWHLVKQLSES 60

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           HPCIIPSFGLHPWYVP RT GWLEKLKEFFEAVP+AAVGEIGLDKGS+G+EINF DQ+EV
Sbjct: 61  HPCIIPSFGLHPWYVPERTTGWLEKLKEFFEAVPTAAVGEIGLDKGSKGKEINFIDQVEV 120

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLE+PASIHCVRAFGDLL IMKSMGPFPAGFILHSYLGSA+MVPEFSKLGA
Sbjct: 121 FQQQLELAKSLERPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGA 180

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSK +SLY VEGDPSL E LK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKLDSLYFVEGDPSLPECLK 240

Query: 241 AQEQS-APTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSY 300
            QEQS A TGSQQSTL  SSTDASTLP+ TLNHPANIQNVL YVASLL ISKEELAELSY
Sbjct: 241 TQEQSAAQTGSQQSTLPGSSTDASTLPKGTLNHPANIQNVLVYVASLLDISKEELAELSY 300

Query: 301 RNAEHLFSFNGVQTS 315
           RNAE LFSF G++TS
Sbjct: 301 RNAERLFSFTGLETS 315

BLAST of CSPI03G09900 vs. NCBI nr
Match: XP_011650815.2 (uncharacterized protein LOC101220251 isoform X1 [Cucumis sativus])

HSP 1 Score: 628.6 bits (1620), Expect = 2.8e-176
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL
Sbjct: 45  MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 104

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           HPCI+PSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV
Sbjct: 105 HPCIVPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 164

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA
Sbjct: 165 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 224

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK
Sbjct: 225 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 284

Query: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300
           AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR
Sbjct: 285 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 344

Query: 301 NAEHLFSFNGVQTS 315
           NAEHLFSFNGVQTS
Sbjct: 345 NAEHLFSFNGVQTS 358

BLAST of CSPI03G09900 vs. NCBI nr
Match: XP_004133940.1 (uncharacterized protein LOC101220251 isoform X2 [Cucumis sativus] >XP_031738316.1 uncharacterized protein LOC101220251 isoform X2 [Cucumis sativus] >KAE8650276.1 hypothetical protein Csa_011695 [Cucumis sativus])

HSP 1 Score: 628.6 bits (1620), Expect = 2.8e-176
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL
Sbjct: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           HPCI+PSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV
Sbjct: 61  HPCIVPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240

Query: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300
           AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR
Sbjct: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300

Query: 301 NAEHLFSFNGVQTS 315
           NAEHLFSFNGVQTS
Sbjct: 301 NAEHLFSFNGVQTS 314

BLAST of CSPI03G09900 vs. NCBI nr
Match: XP_008438219.1 (PREDICTED: uncharacterized metal-dependent hydrolase YabD isoform X1 [Cucumis melo] >XP_008438220.1 PREDICTED: uncharacterized metal-dependent hydrolase YabD isoform X1 [Cucumis melo] >XP_008438221.1 PREDICTED: uncharacterized metal-dependent hydrolase YabD isoform X1 [Cucumis melo] >XP_016898899.1 PREDICTED: uncharacterized metal-dependent hydrolase YabD isoform X1 [Cucumis melo])

HSP 1 Score: 611.7 bits (1576), Expect = 3.6e-171
Identity = 305/314 (97.13%), Postives = 307/314 (97.77%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           MAKLSSTLRLFDAHCHLQDPRIFDKAPQLIS AVDSGVVRFAVNG SEKDWHLVKQLSEL
Sbjct: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISVAVDSGVVRFAVNGTSEKDWHLVKQLSEL 60

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           HPCI+PSFGLHPWYVPGRTP WLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV
Sbjct: 61  HPCIVPSFGLHPWYVPGRTPVWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLEKPASIHCVRAFGDLL IMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLKIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSMEAKKAKKMLK VPSDRILLETDAPDALPKSKSNSLYLVEGDP+LTEFLK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKTVPSDRILLETDAPDALPKSKSNSLYLVEGDPALTEFLK 240

Query: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300
           AQEQSAPTGSQQSTLLCSS DASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR
Sbjct: 241 AQEQSAPTGSQQSTLLCSSADASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300

Query: 301 NAEHLFSFNGVQTS 315
           NAEHLFSF GVQTS
Sbjct: 301 NAEHLFSFKGVQTS 314

BLAST of CSPI03G09900 vs. NCBI nr
Match: KAA0049069.1 (putative metal-dependent hydrolase YabD isoform X1 [Cucumis melo var. makuwa] >TYK17493.1 putative metal-dependent hydrolase YabD isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 604.4 bits (1557), Expect = 5.7e-169
Identity = 301/310 (97.10%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           MAKLSSTLRLFDAHCHLQDPRIFDKAPQLIS AVDSGVVRFAVNG SEKDWHLVKQLSEL
Sbjct: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISVAVDSGVVRFAVNGTSEKDWHLVKQLSEL 60

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           HPCI+PSFGLHPWYVPGRTP WLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV
Sbjct: 61  HPCIVPSFGLHPWYVPGRTPVWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLEKPASIHCVRAFGDLL IMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLKIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSMEAKKAKKMLK VPSDRILLETDAPDALPKSKSNSLYLVEGDP+LTEFLK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKTVPSDRILLETDAPDALPKSKSNSLYLVEGDPALTEFLK 240

Query: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300
           AQEQSAPTGSQQSTLLCSS DASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR
Sbjct: 241 AQEQSAPTGSQQSTLLCSSADASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300

Query: 301 NAEHLFSFNG 311
           NAEHLFSF G
Sbjct: 301 NAEHLFSFKG 310

BLAST of CSPI03G09900 vs. NCBI nr
Match: XP_038880364.1 (uncharacterized metal-dependent hydrolase YabD isoform X2 [Benincasa hispida])

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-165
Identity = 295/314 (93.95%), Postives = 306/314 (97.45%), Query Frame = 0

Query: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSEL 60
           MAKLS++LRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRF+VNGISEKDWHLVKQLSE 
Sbjct: 1   MAKLSASLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFSVNGISEKDWHLVKQLSES 60

Query: 61  HPCIIPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120
           +PCI+PSFGLHPW VP RT GWLEKLKEFFEAVPSAAVGEIGLDKGSRG+EINFTDQIEV
Sbjct: 61  YPCIVPSFGLHPWSVPERTRGWLEKLKEFFEAVPSAAVGEIGLDKGSRGKEINFTDQIEV 120

Query: 121 FQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180
           FQQQLELAKSLE+PASIHCVRAFGDLL IMKSMGPFPAGFILHSYLGSA+MVPEFSKLGA
Sbjct: 121 FQQQLELAKSLERPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGA 180

Query: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLK 240
           YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKS+SLYLVEGDPSL+EFLK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSDSLYLVEGDPSLSEFLK 240

Query: 241 AQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300
            QEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLL ISKEELAELSYR
Sbjct: 241 TQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLDISKEELAELSYR 300

Query: 301 NAEHLFSFNGVQTS 315
           NAEHLFSF G+QTS
Sbjct: 301 NAEHLFSFKGLQTS 314

BLAST of CSPI03G09900 vs. TAIR 10
Match: AT5G17570.1 (TatD related DNase )

HSP 1 Score: 417.2 bits (1071), Expect = 1.2e-116
Identity = 203/303 (67.00%), Postives = 243/303 (80.20%), Query Frame = 0

Query: 8   LRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIIPS 67
           ++LFDAHCHLQDPRI  KAPQ+IS+AV SGV  FAVNG+SEKDW LVK++   +P ++P 
Sbjct: 1   MKLFDAHCHLQDPRIITKAPQIISSAVASGVSAFAVNGVSEKDWSLVKEMGAKYPSVVPC 60

Query: 68  FGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEVFQQQLEL 127
           FG+HPWYV  R+P W E LK FFE  P+AAVGEIGLDKGS+GREI+F++Q+ VF+QQLEL
Sbjct: 61  FGIHPWYVAERSPQWFETLKSFFETTPTAAVGEIGLDKGSKGREIDFSEQVTVFRQQLEL 120

Query: 128 AKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGAYFSFSGF 187
           AK L+KPAS+HCVRAFGDLL I+KS+GPFP+G +LHSYLGSAEMVPEF+KLGAYFSFSGF
Sbjct: 121 AKELKKPASVHCVRAFGDLLEILKSVGPFPSGVMLHSYLGSAEMVPEFAKLGAYFSFSGF 180

Query: 188 LMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLKAQEQSAP 247
           LMSM  KKAKKMLK VPSDRILLETD+PDALPK++S  LY V+GDPSL E   + +    
Sbjct: 181 LMSMSEKKAKKMLKAVPSDRILLETDSPDALPKAESGCLYFVDGDPSLPEEGNSAQDLDS 240

Query: 248 TGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYRNAEHLFS 307
               +  +   S+D+  L +ETLNHPANI  VL YVA LL +  EELAELSY+NA  LFS
Sbjct: 241 ASYDKPNV---SSDSMKLTKETLNHPANIHIVLGYVAQLLDMKNEELAELSYQNAVRLFS 300

Query: 308 FNG 311
           + G
Sbjct: 301 YEG 300

BLAST of CSPI03G09900 vs. TAIR 10
Match: AT3G03500.1 (TatD related DNase )

HSP 1 Score: 331.3 bits (848), Expect = 8.7e-91
Identity = 164/303 (54.13%), Postives = 209/303 (68.98%), Query Frame = 0

Query: 8   LRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIIPS 67
           ++LFDAHCHLQD R+ DKA QLISAA+  GV  FAVNG SEKDW+LVK++ E +P ++P 
Sbjct: 1   MKLFDAHCHLQDKRVIDKASQLISAALAVGVTNFAVNGTSEKDWNLVKEMGETYPSVVPC 60

Query: 68  FGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEVFQQQLEL 127
           FGLHPW++  R+P W + LK+FFE  P+AAVGEIGLDKG     I+++DQ+ VF+ QLEL
Sbjct: 61  FGLHPWFIADRSPHWFKTLKKFFETTPTAAVGEIGLDKGPLAGGIDYSDQLVVFRPQLEL 120

Query: 128 AKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGAYFSFSGF 187
           AK L KP ++HC+ AF DLL IM+S+GPFPAG ILHS+ GSAE+VP+ ++LGAYFSFSG+
Sbjct: 121 AKELNKPVAVHCIDAFDDLLEIMRSIGPFPAGVILHSFNGSAEVVPKLAELGAYFSFSGW 180

Query: 188 LMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFLKAQEQSAP 247
              ++ K AKK LK +PSDR+LLETD+PD LPK                           
Sbjct: 181 FTYIDEKIAKKTLKSIPSDRLLLETDSPDGLPK--------------------------- 240

Query: 248 TGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYRNAEHLFS 307
                      S ++S+ P+ TLN PANI  VLDYVA+L  + KEELAELSY N+  LFS
Sbjct: 241 -----------SDESSSDPKPTLNEPANILAVLDYVANLSNMKKEELAELSYVNSVRLFS 265

Query: 308 FNG 311
           + G
Sbjct: 301 YPG 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P375452.0e-2328.15Uncharacterized metal-dependent hydrolase YabD OS=Bacillus subtilis (strain 168)... [more]
Q05AV05.1e-1927.01Putative deoxyribonuclease tatdn3-A OS=Xenopus laevis OX=8355 GN=tatdn3-a PE=2 S... [more]
Q8K9J11.1e-1827.90Uncharacterized metal-dependent hydrolase BUsg_343 OS=Buchnera aphidicola subsp.... [more]
P733355.6e-1825.17D-aminoacyl-tRNA deacylase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11... [more]
E6WHK11.6e-1727.103'-5' ssDNA/RNA exonuclease TatD OS=Pantoea sp. (strain At-9b) OX=592316 GN=tatD... [more]
Match NameE-valueIdentityDescription
A0A0A0L4G21.4e-17699.68Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G127010 PE=4 SV=1[more]
A0A1S3AWG81.7e-17197.13uncharacterized metal-dependent hydrolase YabD isoform X1 OS=Cucumis melo OX=365... [more]
A0A5A7U6E32.8e-16997.10Putative metal-dependent hydrolase YabD isoform X1 OS=Cucumis melo var. makuwa O... [more]
A0A6J1CYM12.9e-15890.76uncharacterized protein LOC111015984 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A6J1HP041.0e-15589.52uncharacterized protein LOC111464387 OS=Cucurbita moschata OX=3662 GN=LOC1114643... [more]
Match NameE-valueIdentityDescription
XP_011650815.22.8e-17699.68uncharacterized protein LOC101220251 isoform X1 [Cucumis sativus][more]
XP_004133940.12.8e-17699.68uncharacterized protein LOC101220251 isoform X2 [Cucumis sativus] >XP_031738316.... [more]
XP_008438219.13.6e-17197.13PREDICTED: uncharacterized metal-dependent hydrolase YabD isoform X1 [Cucumis me... [more]
KAA0049069.15.7e-16997.10putative metal-dependent hydrolase YabD isoform X1 [Cucumis melo var. makuwa] >T... [more]
XP_038880364.11.3e-16593.95uncharacterized metal-dependent hydrolase YabD isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G17570.11.2e-11667.00TatD related DNase [more]
AT3G03500.18.7e-9154.13TatD related DNase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.140coord: 8..248
e-value: 2.1E-55
score: 189.8
NoneNo IPR availablePANTHERPTHR47176:SF1OSJNBA0020J04.13 PROTEINcoord: 11..249
NoneNo IPR availablePANTHERPTHR47176OSJNBA0020J04.13 PROTEINcoord: 11..249
IPR001130TatD familyPFAMPF01026TatD_DNasecoord: 16..244
e-value: 1.3E-51
score: 175.5
IPR001130TatD familyPIRSFPIRSF005902DNase_TatDcoord: 24..248
e-value: 3.7E-43
score: 145.5
IPR001130TatD familyCDDcd01310TatD_DNAsecoord: 11..244
e-value: 9.74361E-46
score: 151.572
IPR032466Metal-dependent hydrolaseSUPERFAMILY51556Metallo-dependent hydrolasescoord: 11..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G09900.1CSPI03G09900.1mRNA
CSPI03G09900.2CSPI03G09900.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds