CSPI03G09860 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G09860
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionEF-hand domain-containing protein
LocationChr3: 7961458 .. 7964902 (+)
RNA-Seq ExpressionCSPI03G09860
SyntenyCSPI03G09860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCCAACACAGAAAGGAAATGATGAACGGCAGAAACAGAATAGTTTCTGAATTTTTATCCCTTTTTTTTCTATTACAAAACCACTGGTTATGATAAATCTCTTTATCTTTGCAGCTACCGGCTCTCATCTCAATCTTCATTTGCGAGGTCCGTGATCGGCGCCGGCATCTAGCATGGGACAGGCAATGGGGATATTCGGAGGTAAAGTTTTGACCTAACTTCATCTTCTTCCTAAACTTCCATTTCAATTCCTATTCGTTCTTAGCTTTCTGTTTGTATCCCGTTGATTAATCGATTAACGGATGGAGTGAATTAGCGAAACGAAAACTTCATAGTCGTATTCGATCTTATTAATGAAATTCGATTTTCTTTTGTTTTGTTTGATGTGTTGAATTATGAGTTTTTTTCTTTTCACGATGATGTGATTACAAAAATGTTCGTTCATGTGGATTGACCTCTTTGCTATCACTCTCCTCTCCTTTTTTTTTCTTTTCATCTCCCTCTCCCTCTCCCTCTCCCTCATTTAAAAATAGACCTTTTCGTTTTTTTTGTATAAACATAGAGTTATTTGATTCTGAGTTTTCAAATTTCGGTCCTTTAGTTTTTAAAAATAAATTTAAAAGCTTAAAAAATAACTATACTTTTATTTTAAATAGTTATATGACATTTTAAATAATTCAAATTGACTTCTAAAAATCATATTTTCACTCAAAACTATTTTTAAAAATTTAAATGTCATATAATTATTTTTAAAAATATAAAAAATAAATACTACTTTATAACCCCATTTACAGAAAAATAAGGAACTAAAAAGTTATACTAGGTGGGTCTAGTGTTATAAACCAAAACACTAAAAAGAATTTATTTTTAAGATCTAGGGACCTAGTACATATTAATCAAAAACTTGTGGACTAAAAAGTATTTTCTGTAGACTAAAGTATTCATTTTAAAGCTTCATGGATCAAATAGAATTCACCTTATTTTACTAATAAAAGAAAAGAGGAAGAGTAATTCAGAATGATATAAGTATTATTCAAAATATATAAAAAAAAAATCATGACTCATAAAAAGTAGTTAAAAAAAACACATTTTAGGTAATGAATTTGGGTCAAATTGGATGAGATGTTTGCAATTGCAGGAAAAGATTGGAGGAAAAATCAATTGAAGAAGATAACGGACAGAATATTTAAAATATTTAGCAAAGGTAGTGATAAGATGTCGTGTCAAGCTCTGAAAGAAGCTACCTTACATGTATACAAGTAAGAATTTCTACTCTCTTTTTTCTTTTTGTCTTGCTTCACTTCTATAGTTCATTCTTTCATTCACAACATACATATTTGGTGGGACCTCAAAACGTGTAACCCTTTTTTATTATTTTTTTGGAAAATTGCATTAGATAACAGAAAGAAAAAAAAAACTCACAGCATCTTTTTTTTTTTTTTTGCATATTGTAAATTTGATAAATATGATCGGTAATCGATGCTATTAGACGATAATTTGCTTTTAAATTTGTTATTTTTGCAATTTAGAAAAAGTAGGTGACATGGGCTTTGTTATCATAATTTTTTTTTTGCTATTTTTGCAAACTTTCTTTAATTTTATCGAAATTGATTAATAATAATAATAATTTTCATGTAAATGTTTGCTTTTAAATATAACAAAATGAAAAAAAAAATTATTTACAAAATATCATAGACTATTTACGATGGAATAAGATAGATTGTTGTCTGTGTTTATTCGTATCGTGATATACACGTATAATAATCTATTTTAGTCGAACGAATAATATTTTGCTATTTTTATATAAAGTTAACTGATAACAAAAAAAAAACAAAAAACAAAAGACTTTGGAATCGCTATTTGTCGGTTAAAAGTATAAAACTAAAACTAAACCAACATTGAAATATCAAAACTAAATTAGTATTTTAACATTTGTGAGGTTTGATAAGGTAATGATTAGGTTAATTCTATCTATGTGCTCTTCATCTTTCCATTGTTAAAAAACAAGACTAAAAGCTTAAGAAAAAAACTAGGGTTTGGTATCTGTTGTTCTTTTTTCTTTTTCCTTGTCTCTTTCTTCTTCCAAATTTTAAAAATTCAAATTATTACATAAAGACCTATTTCTTTTAATTAAACATTAAAAGAAAATTATGTAATATTTGTATTATACATCTCATGAGTAAATAGATAAAATGATGCTTTTATAAATGTCTTGAAAATAAATTGAAAGCCTGACTAAAAAAATTTGATTTCACCATTTGAAATTCCTATAAAAGGTTAGAAAAGTTACCAATTTTTATGTTACTCAAAATTAAAAAATTGAAAATAGGAAACGAGAAAGGTAAATGTTATCAAATTGTTCTTAAAATTTCGAATGATTGTGCTTCAACCTTGATATTTAGAAATATTTGATTAAAATCTCGAGCTTTTTGGTGATTTGGCATTTTTAGGCTGGTTTTAATACCGAGTGTATGAATGTGATGCAGTGATATCAATAAGCATTGGCCCGGACCACATTTTAGTCCTCCCATGACGGAAGACTTTGATCAAATCGTCGAGAAAGTCTTAAAGGTACTTTGTTGATTCAATAATAACAAAAGGCTAAACAATAATGTTGTAAGAAAGTGGAAACCTTTAGTTGCTGGAAATGCATTCCATGGGCTCACCTTTGCTATTTATTGTTTGTTTGTTTAACTGTTTTCAAAGTATTTATCACGATCAAATAAAGATCGTCAAACTAAAATAATTTAAAGTGAAATTAAGAAAGAAAAATTATATCTCATTTAGAACATACCACCAATATTTTGTTGTATCAAATTAGGGGTGTTGCATTTGATAGCTAGTTTGGAGTCGGTTTTCCAACCAAAATGTTATCGAATCGATTATGGTTAGTTTAGTAAACGTTCAAATTGATCCCAACCGTCAAGGAGTGCTAACAAGCCTTCGGTTGATTGGTTAGTTTATTCTTTAAAAGATTTTTAAAAAAAAATATTTGAATTTTCTCCGAACTGACACCAACTACCTTTAAGTATGGTTCAATTCGTTGTGTTATAGGATAGTGATAAGAACAAGGACCAAGTAATTAACAGTGACGAATTTTTGGAGTTCATACTGCACTTGACAACCTATGCGTTTGTAACGGTGACGGGCAAAGTGCCGTTCGTCACTTTGGTGGTTGCACCGACGGTTGCATTGGTGACAAAGAAGAGTACCGAGGGCATTCCGGGTGTTGGAAAACTTGTGCAAAAAATGCCCTCTTCAGCATATGCTTTCCTCGTCACTCTTGTTGTTGTCGCCTTTCAAAACTCTAAACAACGACTTCTACTTAAATGATTAAGTTCACACTGAGTTTCATTCGTTATGTTATTCCATGCATTTATATATATATACATTCCTTTGTTATTTACATGATCTACATATGTGATTGTAAACTTATACTGTAAC

mRNA sequence

TTTCCAACACAGAAAGGAAATGATGAACGGCAGAAACAGAATAGTTTCTGAATTTTTATCCCTTTTTTTTCTATTACAAAACCACTGGTTATGATAAATCTCTTTATCTTTGCAGCTACCGGCTCTCATCTCAATCTTCATTTGCGAGGTCCGTGATCGGCGCCGGCATCTAGCATGGGACAGGCAATGGGGATATTCGGAGGAAAAGATTGGAGGAAAAATCAATTGAAGAAGATAACGGACAGAATATTTAAAATATTTAGCAAAGGTAGTGATAAGATGTCGTGTCAAGCTCTGAAAGAAGCTACCTTACATGTATACAATGATATCAATAAGCATTGGCCCGGACCACATTTTAGTCCTCCCATGACGGAAGACTTTGATCAAATCGTCGAGAAAGTCTTAAAGGATAGTGATAAGAACAAGGACCAAGTAATTAACAGTGACGAATTTTTGGAGTTCATACTGCACTTGACAACCTATGCGTTTGTAACGGTGACGGGCAAAGTGCCGTTCGTCACTTTGGTGGTTGCACCGACGGTTGCATTGGTGACAAAGAAGAGTACCGAGGGCATTCCGGGTGTTGGAAAACTTGTGCAAAAAATGCCCTCTTCAGCATATGCTTTCCTCGTCACTCTTGTTGTTGTCGCCTTTCAAAACTCTAAACAACGACTTCTACTTAAATGATTAAGTTCACACTGAGTTTCATTCGTTATGTTATTCCATGCATTTATATATATATACATTCCTTTGTTATTTACATGATCTACATATGTGATTGTAAACTTATACTGTAAC

Coding sequence (CDS)

ATGGGACAGGCAATGGGGATATTCGGAGGAAAAGATTGGAGGAAAAATCAATTGAAGAAGATAACGGACAGAATATTTAAAATATTTAGCAAAGGTAGTGATAAGATGTCGTGTCAAGCTCTGAAAGAAGCTACCTTACATGTATACAATGATATCAATAAGCATTGGCCCGGACCACATTTTAGTCCTCCCATGACGGAAGACTTTGATCAAATCGTCGAGAAAGTCTTAAAGGATAGTGATAAGAACAAGGACCAAGTAATTAACAGTGACGAATTTTTGGAGTTCATACTGCACTTGACAACCTATGCGTTTGTAACGGTGACGGGCAAAGTGCCGTTCGTCACTTTGGTGGTTGCACCGACGGTTGCATTGGTGACAAAGAAGAGTACCGAGGGCATTCCGGGTGTTGGAAAACTTGTGCAAAAAATGCCCTCTTCAGCATATGCTTTCCTCGTCACTCTTGTTGTTGTCGCCTTTCAAAACTCTAAACAACGACTTCTACTTAAATGA

Protein sequence

MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPHFSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLVVAPTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLLLK*
Homology
BLAST of CSPI03G09860 vs. ExPASy TrEMBL
Match: A0A0A0L4F9 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G126960 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 5.9e-89
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60
           MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH
Sbjct: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60

Query: 61  FSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLVVA 120
           FSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLVVA
Sbjct: 61  FSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLVVA 120

Query: 121 PTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLLLK 171
           PTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLLLK
Sbjct: 121 PTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLLLK 170

BLAST of CSPI03G09860 vs. ExPASy TrEMBL
Match: A0A1S3AWG2 (uncharacterized protein LOC103483386 OS=Cucumis melo OX=3656 GN=LOC103483386 PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 7.7e-65
Identity = 131/172 (76.16%), Postives = 147/172 (85.47%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60
           MGQAMGIFGGK WRKNQLKKITDRIF   S+G D + C+AL+EATL VYNDINK WPGPH
Sbjct: 1   MGQAMGIFGGKGWRKNQLKKITDRIFDRISEGGDTIPCKALQEATLLVYNDINKLWPGPH 60

Query: 61  FSPPMTEDFDQIV----EKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVT 120
           F+PP  E FD++V    EKV+KDSDKNKDQVIN +EFLEFILHLTT A+VTV  +VP +T
Sbjct: 61  FNPPTLEPFDKVVKDYIEKVIKDSDKNKDQVINREEFLEFILHLTTDAYVTVRERVPILT 120

Query: 121 LVVAPTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLL 169
           LVVAPT A+VTKKSTEGIPGVGKLVQK+P+SAYA LVT+V V FQNSKQRLL
Sbjct: 121 LVVAPTAAIVTKKSTEGIPGVGKLVQKLPTSAYALLVTVVAVTFQNSKQRLL 172

BLAST of CSPI03G09860 vs. ExPASy TrEMBL
Match: A0A5A7U613 (Calcium-binding EF-hand OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003630 PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 5.5e-63
Identity = 130/172 (75.58%), Postives = 144/172 (83.72%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60
           MGQAMGIFGGK WRKNQLKKITDRIF   S G D M  +AL+EATL VYNDINK W GPH
Sbjct: 1   MGQAMGIFGGKGWRKNQLKKITDRIFDKISGGGDTMPYKALQEATLLVYNDINKRWHGPH 60

Query: 61  FSPPMTEDFDQIV----EKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVT 120
           F+PP  E FD++V    EKV+KDSDKNKDQVIN +EFLEFILHLTT A+VTV  +VP +T
Sbjct: 61  FNPPTLEPFDKVVKDYIEKVIKDSDKNKDQVINREEFLEFILHLTTDAYVTVRERVPILT 120

Query: 121 LVVAPTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLL 169
           LVVAPT A+VTKKSTEGIPGVGKLVQK+P+SAYA LVT+V V FQNSKQRLL
Sbjct: 121 LVVAPTAAIVTKKSTEGIPGVGKLVQKLPTSAYALLVTVVAVTFQNSKQRLL 172

BLAST of CSPI03G09860 vs. ExPASy TrEMBL
Match: A0A6J1ITL1 (uncharacterized protein LOC111479803 OS=Cucurbita maxima OX=3661 GN=LOC111479803 PE=4 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 8.0e-38
Identity = 92/168 (54.76%), Postives = 121/168 (72.02%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60
           M +A+G FGGK+WR  QL+ I+DRIF +     DK+S + L  ATL VYNDINK  P  H
Sbjct: 1   MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISH 60

Query: 61  FSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLVVA 120
             PP  E F Q+    +K++D N+D+ I  DEFL FIL LT+ AF++++ ++  +TLV+A
Sbjct: 61  LDPPTEERFRQL----MKENDWNEDKKIGRDEFLGFILSLTSDAFISLSHRL-ILTLVIA 120

Query: 121 PTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLL 169
           PTVA+VTK+STEGIP VG LVQK+PSSAYAFLVTL    FQNS++ +L
Sbjct: 121 PTVAVVTKRSTEGIPVVGSLVQKLPSSAYAFLVTLAAFVFQNSQKPML 163

BLAST of CSPI03G09860 vs. ExPASy TrEMBL
Match: A0A5A7U485 (EF-hand domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002950 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 1.0e-37
Identity = 92/168 (54.76%), Postives = 125/168 (74.40%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60
           MGQA+  FGG+DW+  QL+ I+++IF  F K  +K+S + L  ATL V+NDINK+ PGPH
Sbjct: 1   MGQALRRFGGQDWKNKQLEMISNKIFDRFHK--EKLSFEDLYIATLLVFNDINKYLPGPH 60

Query: 61  FSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLVVA 120
             PP  E   ++VEK    SD+N++Q I+ DEFL+FI  LT  +  +V+ ++  +TLVVA
Sbjct: 61  IDPPTKERVAELVEK----SDENENQAIDRDEFLKFIKLLTFESIASVSQRL-IITLVVA 120

Query: 121 PTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLL 169
           PT+A+VTK+STEGIPG+GK+VQK+PSSAYA LVTL  + FQNS Q+LL
Sbjct: 121 PTLAVVTKRSTEGIPGLGKMVQKVPSSAYALLVTLAALLFQNSNQQLL 161

BLAST of CSPI03G09860 vs. NCBI nr
Match: KGN56633.1 (hypothetical protein Csa_009568 [Cucumis sativus])

HSP 1 Score: 336.7 bits (862), Expect = 1.2e-88
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60
           MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH
Sbjct: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60

Query: 61  FSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLVVA 120
           FSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLVVA
Sbjct: 61  FSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLVVA 120

Query: 121 PTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLLLK 171
           PTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLLLK
Sbjct: 121 PTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLLLK 170

BLAST of CSPI03G09860 vs. NCBI nr
Match: XP_008438213.1 (PREDICTED: uncharacterized protein LOC103483386 [Cucumis melo])

HSP 1 Score: 256.5 bits (654), Expect = 1.6e-64
Identity = 131/172 (76.16%), Postives = 147/172 (85.47%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60
           MGQAMGIFGGK WRKNQLKKITDRIF   S+G D + C+AL+EATL VYNDINK WPGPH
Sbjct: 1   MGQAMGIFGGKGWRKNQLKKITDRIFDRISEGGDTIPCKALQEATLLVYNDINKLWPGPH 60

Query: 61  FSPPMTEDFDQIV----EKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVT 120
           F+PP  E FD++V    EKV+KDSDKNKDQVIN +EFLEFILHLTT A+VTV  +VP +T
Sbjct: 61  FNPPTLEPFDKVVKDYIEKVIKDSDKNKDQVINREEFLEFILHLTTDAYVTVRERVPILT 120

Query: 121 LVVAPTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLL 169
           LVVAPT A+VTKKSTEGIPGVGKLVQK+P+SAYA LVT+V V FQNSKQRLL
Sbjct: 121 LVVAPTAAIVTKKSTEGIPGVGKLVQKLPTSAYALLVTVVAVTFQNSKQRLL 172

BLAST of CSPI03G09860 vs. NCBI nr
Match: KAA0049065.1 (Calcium-binding EF-hand [Cucumis melo var. makuwa] >TYK17498.1 Calcium-binding EF-hand [Cucumis melo var. makuwa])

HSP 1 Score: 250.4 bits (638), Expect = 1.1e-62
Identity = 130/172 (75.58%), Postives = 144/172 (83.72%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGSDKMSCQALKEATLHVYNDINKHWPGPH 60
           MGQAMGIFGGK WRKNQLKKITDRIF   S G D M  +AL+EATL VYNDINK W GPH
Sbjct: 1   MGQAMGIFGGKGWRKNQLKKITDRIFDKISGGGDTMPYKALQEATLLVYNDINKRWHGPH 60

Query: 61  FSPPMTEDFDQIV----EKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVT 120
           F+PP  E FD++V    EKV+KDSDKNKDQVIN +EFLEFILHLTT A+VTV  +VP +T
Sbjct: 61  FNPPTLEPFDKVVKDYIEKVIKDSDKNKDQVINREEFLEFILHLTTDAYVTVRERVPILT 120

Query: 121 LVVAPTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLL 169
           LVVAPT A+VTKKSTEGIPGVGKLVQK+P+SAYA LVT+V V FQNSKQRLL
Sbjct: 121 LVVAPTAAIVTKKSTEGIPGVGKLVQKLPTSAYALLVTVVAVTFQNSKQRLL 172

BLAST of CSPI03G09860 vs. NCBI nr
Match: XP_038892374.1 (uncharacterized protein LOC120081499 [Benincasa hispida])

HSP 1 Score: 184.1 bits (466), Expect = 1.0e-42
Identity = 101/170 (59.41%), Postives = 125/170 (73.53%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSK--GSDKMSCQALKEATLHVYNDINKHWPG 60
           MGQA+  FGGK+WR  QL+KITDRIF  F K   SDK+  + L  ATL VYN+INK  PG
Sbjct: 1   MGQALWTFGGKEWRDKQLRKITDRIFDKFQKQTHSDKLPAEDLYIATLLVYNEINKLIPG 60

Query: 61  PHFSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLV 120
           PH  PP TE   + V+++++ SD N DQ I+  EFL FIL LT+  F +V+ ++  +TLV
Sbjct: 61  PHRDPPTTERVKERVKELIQKSDMNGDQYIDRGEFLHFILSLTSETFKSVSQRLSILTLV 120

Query: 121 VAPTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLL 169
           VAPTVA+VTKKSTE IPGVGKLVQK+P SAYAFLVTL  + FQ S+Q+LL
Sbjct: 121 VAPTVAVVTKKSTEAIPGVGKLVQKLPVSAYAFLVTLAALIFQKSQQQLL 170

BLAST of CSPI03G09860 vs. NCBI nr
Match: XP_038876686.1 (uncharacterized protein LOC120069050 [Benincasa hispida])

HSP 1 Score: 181.8 bits (460), Expect = 5.0e-42
Identity = 97/170 (57.06%), Postives = 133/170 (78.24%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIFSKGS--DKMSCQALKEATLHVYNDINKHWPG 60
           MGQ++G FGGK+WR  QL+KI+++IF  F   S  DK+S + L  ATL VYNDINK+ PG
Sbjct: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60

Query: 61  PHFSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLV 120
           PH  PP  E     V+++++DSD+++D+ I+ +EFL+FIL LT+  FV+V+ ++  +TLV
Sbjct: 61  PHLDPPTKER----VKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRL-ILTLV 120

Query: 121 VAPTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVVAFQNSKQRLL 169
           VAPTVA+VTKKSTEG+PG+GK VQK+P+SAYAFLVTL  + FQNS+Q+LL
Sbjct: 121 VAPTVAVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLL 165

BLAST of CSPI03G09860 vs. TAIR 10
Match: AT1G64850.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 132.1 bits (331), Expect = 4.2e-31
Identity = 73/160 (45.62%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 1   MGQAMGIFGGKDWRKNQLKKITDRIFKIF--SKGSDKMSCQALKEATLHVYNDINKHWPG 60
           MGQ      GK+WR  Q++ I DR+F  F    G   ++ + L  A L VYNDINK  PG
Sbjct: 1   MGQVFNKLRGKEWRHKQVQAICDRVFDRFKMQTGRANLTFEELYIAVLLVYNDINKRLPG 60

Query: 61  PHFSPPMTEDFDQIVEKVLKDSDKNKDQVINSDEFLEFILHLTTYAFVTVTGKVPFVTLV 120
           PHF PP  +    +V  ++ D D N D  ++ +EF++FI  +T   F  V  +   + L+
Sbjct: 61  PHFDPPSKD----LVNSIMTDCDINLDGELDREEFVKFIEQITAETF-NVVSQGLIIALI 120

Query: 121 VAPTVALVTKKSTEGIPGVGKLVQKMPSSAYAFLVTLVVV 159
           VAPTVA+ TKK+TEG+PGVGK+VQK+P+S YA LVTL V+
Sbjct: 121 VAPTVAIATKKATEGVPGVGKVVQKLPTSIYASLVTLAVM 155

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0L4F95.9e-89100.00EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G126960 PE=... [more]
A0A1S3AWG27.7e-6576.16uncharacterized protein LOC103483386 OS=Cucumis melo OX=3656 GN=LOC103483386 PE=... [more]
A0A5A7U6135.5e-6375.58Calcium-binding EF-hand OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1ITL18.0e-3854.76uncharacterized protein LOC111479803 OS=Cucurbita maxima OX=3661 GN=LOC111479803... [more]
A0A5A7U4851.0e-3754.76EF-hand domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
Match NameE-valueIdentityDescription
KGN56633.11.2e-88100.00hypothetical protein Csa_009568 [Cucumis sativus][more]
XP_008438213.11.6e-6476.16PREDICTED: uncharacterized protein LOC103483386 [Cucumis melo][more]
KAA0049065.11.1e-6275.58Calcium-binding EF-hand [Cucumis melo var. makuwa] >TYK17498.1 Calcium-binding E... [more]
XP_038892374.11.0e-4259.41uncharacterized protein LOC120081499 [Benincasa hispida][more]
XP_038876686.15.0e-4257.06uncharacterized protein LOC120069050 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G64850.14.2e-3145.63Calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.238.10coord: 15..111
e-value: 2.5E-7
score: 32.5
NoneNo IPR availablePANTHERPTHR11639S100 CALCIUM-BINDING PROTEINcoord: 5..166
NoneNo IPR availablePANTHERPTHR11639:SF133CALCIUM-BINDING EF HAND PROTEINcoord: 5..166
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 81..93
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 68..103
score: 10.552258
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 24..103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G09860.1CSPI03G09860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0048306 calcium-dependent protein binding
molecular_function GO:0005509 calcium ion binding