CSPI03G09730 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G09730
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPectinesterase
LocationChr3: 7888554 .. 7893467 (+)
RNA-Seq ExpressionCSPI03G09730
SyntenyCSPI03G09730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGGAACTCCAAAGGATTCACAAAAATCTCACAACTAAGTAAACCGAGAAAATGGCAAGACACACGATCGTGTTTCTCGTTGTTCTTATTGTTCTTATTTTTCCGTCAGACATTTTATGTTATTCGGAAAAAGAAATAAAAAATTGGTGTAGCCAAACACCATATCCAGCACCATGCGAAGAATTTCTCAAAACCAAAGCTACTACAAAGAAAACTCCAATAACCACAAAATCCCATTTCTTTGAAATCTTAGTCGAAACGGCACTAGAGCGTGCCGTTTCAGCCCATAAAAATGCTCTTTCTCTCGGCCCAAAATGCCGAAATTCCAAAGAAAAAACTGCATGGACCGATTGCGTTGACCTCTACGACCAAATCATAACCCGACTCAACCGAACCTCTGCCCGCTGCTCACCAGCCGACGCTCAAACCTGGCTCAGCGCCGCTCTCACCGCCCTCGAAACCTGCCGGACAGGGTTCGAAGAACTCGGCCTTTCGGCTTTCGGCTACCCATTGACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAACCCGCATCACCGGAGGGGTATGAGCCGACGACCATGACTGATGGGTTTCCCACGTGGGTTAGTCCTGGTAACCGTAAATTGCTGCAATCTGGGTCTCCGAAGGCGGACGTAGTGGTGGCGCAAGATGGGTCTGGGAATTTTAAGACGGTGAAGGATGCTATCTCCGCCGCAAAGGGCGGCGGGAGATTTGTGATATATATAAAGTCGGGGGTTTATAATGAGAATTTGGATATTAAGGCTAAGAATGTTATGATGGTCGGCGACGGAATCGGAAAAACTATCATTACCGGCAGTAGAAGTGTCGGCGGAGGTTCCACCACGTTCAGATCCGCTACCGTTGGTAAGCTCTCTCTCTTTTTCTCTTTAAAACTTTCTTTTTCCTTCAAACTTTTCATTTTTTATTTTCATAAAGTTATTAATTTAAATGGGCCCTTCCAAATATGAGTTCTACATATTACAAATTAGAGAATTTAAGTTAAGATTTAAAAAGCTTTAATTAATATTTGAACTTTAATATGATAACATTAAATAGTGGATAATGGTTGATGTGTGTTGCTAAACCTATTCTTTTTTCGAAAGATGAAAAGTAAAATTCACTAATTCTATTTAACCATATAGATTAGTGGAACAACGAGTCGTTTTTATCAAAATAGGAGTTCGTAGGAGTAGAAAAGTGAAAAAAAGATATTTTTTTAACTCTATAGTTAACTATCAAAATTTTAACATTAATCAATAAGAATTAGCTAAGACTTATGATAGACATAAAATTTAACTTCAAATCAAACATTAATTTTTTAAATAATCTACTTTGGCAACTTCCCCTTAATTAAATAGGATTAGTGTGATAATACGAGGTCTTGAAATTTATTATAAACTTAGTTGTTGTCATTGTATTTATTATAAACTTGCTTGTAGTGCATGGCGCTACCATCATCTCATATGTCACGATGTGTCGGGAAAGTCATTGATTTAGGGAAGATAAAAACACAATAATATTAAATTTTATGTATTATATTATTGGATTTTAAAAAAAAATCAAAATAACATTAGAAAAAGGTTAAATGTACCTAATACATATGATAATGTCATGTAAGTTGACTTTATATATAAACTAATCTATGGATGAAAATTCACAATGTCACATTATGAATTTTTAGTTTCAATTTAATATTAATCGACGACCAATAGGAAATCAACAAACCTACTTTGAGTAACTTAGTAAATTTTGCTGACTGATTTAATTGCTAATAGTAATTAATATAGTTTTGAATTGTTCTTCATTGCCAAACCCTATAATTCTATGTTTTATAACTTTCTCGAGAATTTATTCATTTCTATTGGTTGTAATTAATTTTGGGGTTGAATTATATGCACTTAAGTGACTTTTATTACAATATATTTAGAAATGTGAATGGTATGACCCTCTATTTAATTAATTATAGTTGTAGTAATTAAAAATTGACGTGTGAAATAATTATACGGTAGTTTTAGTTTAAACTTTTAAGAAACCCTACAAAGTTATAGCTACAATTAGAGTAATACTTTTTAAGGTTTATATTTAATTCGGTTTCTAATTGAATTTCAAAAAATGACTATTTTGGTCCTTTTCTTTTCATTTTCTTAGTACAAGTGGAGAACGAAAGATTTGAACCTAATTAAAATCCTTTGGATTTGAGTACATACTTGTTACATTTTAACTAAACTTTTAATGAACTTTTTGTTTTCATTTTTGAGGGATCAAAATTGTTACTTTCTTGAAATGAAAGAATCAAATATATATAAAAATGTATAGAAATGTATGAAAATGTATAGAAATCAAAATGCACATTTTTAAAATACAAAGACCAAGAGAATAGGGTTAAAAGTACAGAGATCATGAAAATTAGTAATATGATGTCATTTTAAAGATCAAATAAACACATTATTATTGGTTAGGAAAGGTATATAAATAATTGAATTTAACTTGAGCATAAAGCTATCAAATTGGAATAATTTGGAGGGATCTATTTGGCATTCAAATCATTTTTATAACAAAGTTGATATAATTTGTTGTTACTCAAAATAATTGGATAAGGTTTTGGAAAAACAAGGTATAATTTATAGTAAAAAGGGAAAGAATAATTAATATAAAAAGTGAACCCACAATGTGTTTCCTAATTATATATATGGGTTAGAATTTATAGAAAAAGAATAGAAATAGAAATAGAAATATTTGATGTGGCATTTTGTTTGAATTATTGAAGTGGAAATGATATCTATAGGGCCATATTTTGATTTAGGGTTTAATGTGGGATGTTAGAGAAAATTATACATATATATATATTATATGATATGACAAAAAGATCAAGTAGCTTGGTTGGTTGGTGTGTAATTTAATGGGACCAAAGTTTCTAATTATTTTACTATATATATATATGCACACACACTCATATATATTAAAAAGAAATAAAAGAAGGGGTAGCATAATAGAATCTATTAAAATTAGTTGAATTTAGCTTTATAAAGCAAGAAAGTGACATGTTTTGAATTCATTCATCACTATTCAACTTTTGTGATTTGTAGTTTTCATCCAAAAGAATATTTATGTTAATTACAATTTTCCATCTTTTTTATTTTAAAAAGGTATAATAACTAATTATATATATATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATATTACTAGCTAAAATCATAAATATAAAATATTTTGTTATGAGTATATTGTATATAATTCAGAAATTTATAAAAAATATTTATATTTTATAGATAAACCAAATGTAATTTTTTTTTGAAATTTTTTTATTTTTTTAAAAAATCCTATTAGTTTCTACTGATAAACATTTTAGCAATGCATTCATATATATGAGTAATATAATCTGAAACTTTATTATATTTTTTATTATATTATATTTAAAAATGTTCTAAAATAATTAGATTTTGAAGGCATATCTTATCTATCTACTTCATAGAAAAATTATTTAAAATCACTCTCAAATCAAACTCACACCCAATGTCAAACTTAATCATAGCTCAATGAATTAGAAAACAATAATTAACTTACAAATAAGCAATTCACATTCTCAAAATACTTTTTTAATAAATGATTTTTTTAAAAAAAAAAATTGTAATATACAAAAAAAAGAACTAAAACCTTGAAGTGTTTTGTTTGCAAAAATAGCTACAAAATAAACTATAAAATATATTGATGAATTAATTTTGATTGTGAATATTTGTAGGTTATTTAATTTAAAGCAAAATTGTCAAAAAAAAACTAATGAGTCTTATTGTGTTAATTAACTAAATTGTGAACGTCGGTGAAATCACAGCCGTTGATGGAGACGGATTCATCGCCAGAGACATAACTTTCAGAAACACCGCCGGAGCAAAGAACCACCAAGCGGTAGCGCTCCGATCAGGTTCAGATCTCTCAGTCTTCTACCGTTGTGGCTTCGAAGGCTATCAAGACACTCTCTACGTTTACGCCGAACGCCAATTCTACAAACAATGCGACATTTACGGCACCGTCGATTTCATCTTCGGCAATGCCGCCGTCGTACTCCAAGATTGCAACATCATCGCCCGTGATCCTCCGAACAAAACCATCACCCTCACCGCCCAAGGCCGCTCCGATCCGAATCAGAACACCGGAATCTCCATCCACAATTGCCGAATCACATCCTCTGGCGGCCTCAGCGGCGTCAAGGCCTATTTAGGCCGCCCATGGAGGCAATATTCCCGGACGGTTGTTATGAAATCCTCGATCGGAGGATTCATAAGTCCGGCGGGGTGGATGCCGTGGAGCGGTAATTTTGCCCTAAATACGCTGTATTACGCCGAGTATATGAATACGGGGCCGGGAGCGTCGACGGCGAATCGGGTGAACTGGAAGGGTTATCATGTGATTACCAGTGCGTCGGAGGCTTCGAAGTTCACCGTGGGGAATTTCATCGCCGGTGGGTCTTGGTTGCCGAGTACGGGTGTGCCTTTCACATCCGGTCTCTAATTTGGCGGTGTAATTTTGTATGTACTATTAAAAATATCAATGATCGTTTAATTTTTTTAATTATACTTTAATAAACAAGTTTATCATTAAGAAATTAGATTTTATTATATCTTTTAAAAAAATTGATTTTACTTTTCTTTTCGTCTCTCGATTCAAAATGGTTGGGTTTGACAAGAGAATTACCATTCAAGAGGGGTATTGTATTACAATTTACAATGTAAGTTTTTCAAATAAATGAAATATGATTTGTTTGAGT

mRNA sequence

AAAAAGGAACTCCAAAGGATTCACAAAAATCTCACAACTAAGTAAACCGAGAAAATGGCAAGACACACGATCGTGTTTCTCGTTGTTCTTATTGTTCTTATTTTTCCGTCAGACATTTTATGTTATTCGGAAAAAGAAATAAAAAATTGGTGTAGCCAAACACCATATCCAGCACCATGCGAAGAATTTCTCAAAACCAAAGCTACTACAAAGAAAACTCCAATAACCACAAAATCCCATTTCTTTGAAATCTTAGTCGAAACGGCACTAGAGCGTGCCGTTTCAGCCCATAAAAATGCTCTTTCTCTCGGCCCAAAATGCCGAAATTCCAAAGAAAAAACTGCATGGACCGATTGCGTTGACCTCTACGACCAAATCATAACCCGACTCAACCGAACCTCTGCCCGCTGCTCACCAGCCGACGCTCAAACCTGGCTCAGCGCCGCTCTCACCGCCCTCGAAACCTGCCGGACAGGGTTCGAAGAACTCGGCCTTTCGGCTTTCGGCTACCCATTGACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAACCCGCATCACCGGAGGGGTATGAGCCGACGACCATGACTGATGGGTTTCCCACGTGGGTTAGTCCTGGTAACCGTAAATTGCTGCAATCTGGGTCTCCGAAGGCGGACGTAGTGGTGGCGCAAGATGGGTCTGGGAATTTTAAGACGGTGAAGGATGCTATCTCCGCCGCAAAGGGCGGCGGGAGATTTGTGATATATATAAAGTCGGGGGTTTATAATGAGAATTTGGATATTAAGGCTAAGAATGTTATGATGGTCGGCGACGGAATCGGAAAAACTATCATTACCGGCAGTAGAAGTGTCGGCGGAGGTTCCACCACGTTCAGATCCGCTACCGTTGCCGTTGATGGAGACGGATTCATCGCCAGAGACATAACTTTCAGAAACACCGCCGGAGCAAAGAACCACCAAGCGGTAGCGCTCCGATCAGGTTCAGATCTCTCAGTCTTCTACCGTTGTGGCTTCGAAGGCTATCAAGACACTCTCTACGTTTACGCCGAACGCCAATTCTACAAACAATGCGACATTTACGGCACCGTCGATTTCATCTTCGGCAATGCCGCCGTCGTACTCCAAGATTGCAACATCATCGCCCGTGATCCTCCGAACAAAACCATCACCCTCACCGCCCAAGGCCGCTCCGATCCGAATCAGAACACCGGAATCTCCATCCACAATTGCCGAATCACATCCTCTGGCGGCCTCAGCGGCGTCAAGGCCTATTTAGGCCGCCCATGGAGGCAATATTCCCGGACGGTTGTTATGAAATCCTCGATCGGAGGATTCATAAGTCCGGCGGGGTGGATGCCGTGGAGCGGTAATTTTGCCCTAAATACGCTGTATTACGCCGAGTATATGAATACGGGGCCGGGAGCGTCGACGGCGAATCGGGTGAACTGGAAGGGTTATCATGTGATTACCAGTGCGTCGGAGGCTTCGAAGTTCACCGTGGGGAATTTCATCGCCGGTGGGTCTTGGTTGCCGAGTACGGGTGTGCCTTTCACATCCGGTCTCTAATTTGGCGGTGTAATTTTGTATGTACTATTAAAAATATCAATGATCGTTTAATTTTTTTAATTATACTTTAATAAACAAGTTTATCATTAAGAAATTAGATTTTATTATATCTTTTAAAAAAATTGATTTTACTTTTCTTTTCGTCTCTCGATTCAAAATGGTTGGGTTTGACAAGAGAATTACCATTCAAGAGGGGTATTGTATTACAATTTACAATGTAAGTTTTTCAAATAAATGAAATATGATTTGTTTGAGT

Coding sequence (CDS)

ATGGCAAGACACACGATCGTGTTTCTCGTTGTTCTTATTGTTCTTATTTTTCCGTCAGACATTTTATGTTATTCGGAAAAAGAAATAAAAAATTGGTGTAGCCAAACACCATATCCAGCACCATGCGAAGAATTTCTCAAAACCAAAGCTACTACAAAGAAAACTCCAATAACCACAAAATCCCATTTCTTTGAAATCTTAGTCGAAACGGCACTAGAGCGTGCCGTTTCAGCCCATAAAAATGCTCTTTCTCTCGGCCCAAAATGCCGAAATTCCAAAGAAAAAACTGCATGGACCGATTGCGTTGACCTCTACGACCAAATCATAACCCGACTCAACCGAACCTCTGCCCGCTGCTCACCAGCCGACGCTCAAACCTGGCTCAGCGCCGCTCTCACCGCCCTCGAAACCTGCCGGACAGGGTTCGAAGAACTCGGCCTTTCGGCTTTCGGCTACCCATTGACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAACCCGCATCACCGGAGGGGTATGAGCCGACGACCATGACTGATGGGTTTCCCACGTGGGTTAGTCCTGGTAACCGTAAATTGCTGCAATCTGGGTCTCCGAAGGCGGACGTAGTGGTGGCGCAAGATGGGTCTGGGAATTTTAAGACGGTGAAGGATGCTATCTCCGCCGCAAAGGGCGGCGGGAGATTTGTGATATATATAAAGTCGGGGGTTTATAATGAGAATTTGGATATTAAGGCTAAGAATGTTATGATGGTCGGCGACGGAATCGGAAAAACTATCATTACCGGCAGTAGAAGTGTCGGCGGAGGTTCCACCACGTTCAGATCCGCTACCGTTGCCGTTGATGGAGACGGATTCATCGCCAGAGACATAACTTTCAGAAACACCGCCGGAGCAAAGAACCACCAAGCGGTAGCGCTCCGATCAGGTTCAGATCTCTCAGTCTTCTACCGTTGTGGCTTCGAAGGCTATCAAGACACTCTCTACGTTTACGCCGAACGCCAATTCTACAAACAATGCGACATTTACGGCACCGTCGATTTCATCTTCGGCAATGCCGCCGTCGTACTCCAAGATTGCAACATCATCGCCCGTGATCCTCCGAACAAAACCATCACCCTCACCGCCCAAGGCCGCTCCGATCCGAATCAGAACACCGGAATCTCCATCCACAATTGCCGAATCACATCCTCTGGCGGCCTCAGCGGCGTCAAGGCCTATTTAGGCCGCCCATGGAGGCAATATTCCCGGACGGTTGTTATGAAATCCTCGATCGGAGGATTCATAAGTCCGGCGGGGTGGATGCCGTGGAGCGGTAATTTTGCCCTAAATACGCTGTATTACGCCGAGTATATGAATACGGGGCCGGGAGCGTCGACGGCGAATCGGGTGAACTGGAAGGGTTATCATGTGATTACCAGTGCGTCGGAGGCTTCGAAGTTCACCGTGGGGAATTTCATCGCCGGTGGGTCTTGGTTGCCGAGTACGGGTGTGCCTTTCACATCCGGTCTCTAA

Protein sequence

MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL*
Homology
BLAST of CSPI03G09730 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 3.5e-187
Identity = 326/513 (63.55%), Postives = 400/513 (77.97%), Query Frame = 0

Query: 6   IVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFFE 65
           I+  V L++         YS +E+K+WC +TP P PCE FL  K  T  T I   + F++
Sbjct: 5   ILITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQK--TDVTSIKQDTDFYK 64

Query: 66  ILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---SARCSPA 125
           I ++ ALERA +A     +LG KCRN +EK AW DC +LY+  + +LN+T   S  C+  
Sbjct: 65  ISLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKV 124

Query: 126 DAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTT 185
           D QTWLS ALT LETCR   E+LG+  +  PL +NNV+KLIS+ LS+NK      Y   +
Sbjct: 125 DKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVP----YNEPS 184

Query: 186 MTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAK--GGGRFVIYIKS 245
             DGFPTWV PG+RKLLQ+ +P+A++VVAQDGSGN KT+++A++AA   GG R+VIYIK+
Sbjct: 185 YKDGFPTWVKPGDRKLLQT-TPRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKA 244

Query: 246 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 305
           G YNEN+++K KN+M VGDGIGKTIITGS+SVGGG+TTF+SATVAV GD FIARDIT RN
Sbjct: 245 GTYNENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRN 304

Query: 306 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 365
           TAG  NHQAVALRSGSDLSVFYRC FEGYQDTLYV+++RQFY++CDIYGTVDFIFGNAAV
Sbjct: 305 TAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAV 364

Query: 366 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGL----SGVKAYLGRP 425
           VLQ+CNI AR PPN+T TLTAQGR+DPNQ+TGI IHNCR+T++  L    S VK +LGRP
Sbjct: 365 VLQNCNIFARKPPNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRP 424

Query: 426 WRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHV 485
           W+QYSRTV +K+ +   I+PAGWM WSG+FALNTLYYAEYMNTGPG+STANRV W+GYHV
Sbjct: 425 WKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHV 484

Query: 486 ITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           +TS S+ S+FTVGNFIAG SWLP+T VPFTSGL
Sbjct: 485 LTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of CSPI03G09730 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 642.1 bits (1655), Expect = 5.3e-183
Identity = 320/516 (62.02%), Postives = 395/516 (76.55%), Query Frame = 0

Query: 1   MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60
           MA    +   V++ ++  S +  Y++K++K WCSQTP P PCE FL   +  +  PI ++
Sbjct: 2   MAFRAYIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNE--PIKSE 61

Query: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---SA 120
           S F +I ++  L+RA+ A  +A +LGPKCR+++EK AW DC+ LYD  ++++N T   + 
Sbjct: 62  SEFLKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNV 121

Query: 121 RCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEG 180
           +CS  DAQTWLS ALT L+TCR GF ELG++    PL +NNVS L+ + L++NK   P  
Sbjct: 122 KCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINK--VPFN 181

Query: 181 YEPTTMTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIY 240
           Y P    DGFP+WV PG+RKLLQS +PK + VVA+DGSGNFKT+K+AI AA G GRFVIY
Sbjct: 182 YTPPE-KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIY 241

Query: 241 IKSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDIT 300
           +K GVY+ENL+I+ KNVM+ GDGIGKTIITGS+SVGGG+TTF SATVA  GDGFIAR IT
Sbjct: 242 VKQGVYSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGIT 301

Query: 301 FRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGN 360
           FRNTAGA N QAVALRSGSDLSVFY+C FE YQDTLYV++ RQFY+ CD+YGTVDFIFGN
Sbjct: 302 FRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGN 361

Query: 361 AAVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGV----KAYL 420
           AA VLQ+CNI AR P +KT T+TAQGRSDPNQNTGI IHN R+T++  L  V    K YL
Sbjct: 362 AAAVLQNCNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYL 421

Query: 421 GRPWRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKG 480
           GRPWRQYSRTV MK+S+   I P GW+ W GNFAL TL+YAE+ NTGPGAST+ RV W G
Sbjct: 422 GRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPG 481

Query: 481 YHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           + V+ SASEASKFTVG F+AGGSW+PS+ VPFTSGL
Sbjct: 482 FRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of CSPI03G09730 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.6e-134
Identity = 263/536 (49.07%), Postives = 357/536 (66.60%), Query Frame = 0

Query: 1   MARHTIVFL-VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITT 60
           M + +I+FL ++L+VL+    +   +++   +WC +TPYP PC+ + K     ++   T 
Sbjct: 5   MQKLSILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP--TQ 64

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---- 120
            S F  +LVE A++RA+SA     + G  C +SK++    DC+DLY   I +LNRT    
Sbjct: 65  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 124

Query: 121 ------SARCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTAN-NVSKLISDGLS 180
                 +  C+  DAQTWLS ALT  ETCR G  +L ++ F  P+ +N  +S LIS+ L+
Sbjct: 125 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLA 184

Query: 181 VNKPASPEGYEPTTMTD--GFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAIS 240
           VN      G +  T  +  GFPTW+S  +++LL+  + +A++VVA+DGSG+F TV+ AI 
Sbjct: 185 VNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAID 244

Query: 241 AA----KGGGRFVIYIKSGVYNENLDIKAKN--VMMVGDGIGKTIITGSRSVGGGSTTFR 300
            A       GRFVIY+K G+Y EN++++  N  +M+VGDG+  TIITG RSV GG TT+ 
Sbjct: 245 VAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYN 304

Query: 301 SATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQ 360
           SAT  ++G  FIA+ ITFRNTAG    QAVALRS SDLS+FY+C  EGYQDTL V+++RQ
Sbjct: 305 SATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQ 364

Query: 361 FYKQCDIYGTVDFIFGNAAVVLQDCNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCR 420
           FY++C IYGTVDFIFGNAA V Q+C I+ R P   +   +TAQGR+DP QNTGISIHN R
Sbjct: 365 FYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSR 424

Query: 421 ITSSGGL----SGVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWMPW--SGNFALNTLYY 480
           I  +  L      VK Y+GRPW ++SRTVV+++ +   +SP GW PW     F L+TL+Y
Sbjct: 425 ILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFY 484

Query: 481 AEYMNTGPGASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           AEY NTGP +ST  RV+WKG+HV+  AS+AS FTVG FIAG +WLP TG+PFTSGL
Sbjct: 485 AEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of CSPI03G09730 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 4.2e-132
Identity = 264/528 (50.00%), Postives = 343/528 (64.96%), Query Frame = 0

Query: 7   VFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEE-FLKTKATTKKTPITTKSHFFE 66
           + L++L+ L   + +   +   I  WC +TPYP PC+  F+K       T I   S F  
Sbjct: 16  LLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQI---SEFRV 75

Query: 67  ILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNR--------TSA 126
           +LVE A++RAVSA     +    C + K++    DC++LY   + +LNR        T  
Sbjct: 76  LLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGR 135

Query: 127 RCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTAN-NVSKLISDGLSVNKPASPE 186
           RC+  DAQTWLS ALT  ETCR G  +L +S F  P+ +N  +S LIS+ L+VN      
Sbjct: 136 RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTA 195

Query: 187 GYEPTTMTD--GFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAA----KG 246
           G   +T  D  GFPTWVS   R+LLQ  S +A++VVA+DGSG+FKTV+ AI  A      
Sbjct: 196 GKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVT 255

Query: 247 GGRFVIYIKSGVYNENLDIKAK--NVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDG 306
            GRFVIY+K G+Y ENL+++    N+M+VGDG+  TIITG RSV GG TT+ SAT  ++G
Sbjct: 256 SGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEG 315

Query: 307 DGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIY 366
             FIA+ I F+NTAG    QAVALRS SDLS+FYRC  EGYQDTL V+++RQFY++C IY
Sbjct: 316 LHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIY 375

Query: 367 GTVDFIFGNAAVVLQDCNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLS 426
           GTVDFIFGNAAVV Q+C I+ R P   +   +TAQGR+D  QNTGISIHN  I  +  L 
Sbjct: 376 GTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLK 435

Query: 427 ----GVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWMPWS--GNFALNTLYYAEYMNTGP 486
                VK Y+GRPW  YSRTVV+K+ I   +SP GW PW+    + L+TL+YAEY N GP
Sbjct: 436 PVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGP 495

Query: 487 GASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
            +ST  RV WKG+HV++ AS+AS F+VG FIAG +WLP +G+PFTS L
Sbjct: 496 ASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of CSPI03G09730 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 2.2e-128
Identity = 250/508 (49.21%), Postives = 331/508 (65.16%), Query Frame = 0

Query: 32  WCSQTPYPAPCEEFLK--TKATTKKTPITTKSHFFEILVETALERAVSAHKNALSLGPKC 91
           WC++TP+   C  + +  ++      P   +S F  +LV+ AL++AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 92  RNSKEKTAWTDCVDLYDQIITRLNRT----------SARCSPADAQTWLSAALTALETCR 151
            N++ K AW+DCV+L+   + +LNRT            +C+  DAQTWLS A T +ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 152 TGFEELGLSAFGYPLTAN-NVSKLISDGLSVNKPASPEGYEPTTMT--DGFPTWVSPGNR 211
           +G E+L +S F  P+ +N N+S LI + L+VN     +    TT    + FP+WVS   R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 212 KLLQSGS---PKADVVVAQDGSGNFKTVKDAISAA---KGGGRFVIYIKSGVYNENLDI- 271
           +LL S S       +VVAQD SG+F++++ AI+ A   +   RFVIY+K GVY EN+D+ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 272 -KAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQ 331
               N+M+VGDG  KTIIT  RSV  G TT+ SAT    G  F+A+D+TF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 332 AVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNII 391
           AVA+RS SDLSVFYR G  G+QDTLY++++RQF+++C I GT+DFIFGNAAVV Q+C I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 392 ARDP-PNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGV----KAYLGRPWRQYSRT 451
            R P   +   +TAQGR DP QNTGI+IH+ RI ++  L  V    K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 452 VVMKSSIGGFISPAGWMPW--SGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSAS 510
            +MK+ I   ISP GW PW    NFALNT++Y EY N GPG+ST  RV WKG+H ITS +
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

BLAST of CSPI03G09730 vs. ExPASy TrEMBL
Match: A0A0A0L7N4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.2e-294
Identity = 507/509 (99.61%), Postives = 507/509 (99.61%), Query Frame = 0

Query: 1   MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60
           MARHTIVF VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK
Sbjct: 1   MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60

Query: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS 120
           SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS
Sbjct: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS 120

Query: 121 PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP 180
           PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP
Sbjct: 121 PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP 180

Query: 181 TTMTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS 240
           TTMTDGFPTWVSPGNRKLLQS SPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS
Sbjct: 181 TTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS 240

Query: 241 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 300
           GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN
Sbjct: 241 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 300

Query: 301 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 360
           TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV
Sbjct: 301 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 360

Query: 361 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY 420
           VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY
Sbjct: 361 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY 420

Query: 421 SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA 480
           SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA
Sbjct: 421 SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA 480

Query: 481 SEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SEASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of CSPI03G09730 vs. ExPASy TrEMBL
Match: A0A5A7U603 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002830 PE=3 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.2e-283
Identity = 487/511 (95.30%), Postives = 503/511 (98.43%), Query Frame = 0

Query: 1   MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKK-TPITT 60
           MARH+IVFLVVL+VLIFP D+LCYSEKEIKNWCSQTPYPAPCEEFLKTKA+TKK TPITT
Sbjct: 1   MARHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITT 60

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARC 120
           KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYD+IITRLNRTS RC
Sbjct: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRC 120

Query: 121 SPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180
           +PADAQTWLSAALTALETCRTGF+ELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE
Sbjct: 121 TPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180

Query: 181 PTT-MTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 240
           PTT M DGFPTWV PG+RKLLQSGSPKA+VVVAQDGSGNFKTVK+A++AAKGGGRFVIY+
Sbjct: 181 PTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 240

Query: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300
           KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300

Query: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360

Query: 361 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWR 420
           AVVLQDCNIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCRITS+GGLSGVKAYLGRPWR
Sbjct: 361 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 420

Query: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVIT 480
           QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYY EYMNTGPGASTANRVNWKGY VIT
Sbjct: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 480

Query: 481 SASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SA+EASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of CSPI03G09730 vs. ExPASy TrEMBL
Match: A0A1S3AVW4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.2e-283
Identity = 487/511 (95.30%), Postives = 503/511 (98.43%), Query Frame = 0

Query: 1   MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKK-TPITT 60
           MARH+IVFLVVL+VLIFP D+LCYSEKEIKNWCSQTPYPAPCEEFLKTKA+TKK TPITT
Sbjct: 1   MARHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITT 60

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARC 120
           KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYD+IITRLNRTS RC
Sbjct: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRC 120

Query: 121 SPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180
           +PADAQTWLSAALTALETCRTGF+ELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE
Sbjct: 121 TPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180

Query: 181 PTT-MTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 240
           PTT M DGFPTWV PG+RKLLQSGSPKA+VVVAQDGSGNFKTVK+A++AAKGGGRFVIY+
Sbjct: 181 PTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 240

Query: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300
           KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300

Query: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360

Query: 361 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWR 420
           AVVLQDCNIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCRITS+GGLSGVKAYLGRPWR
Sbjct: 361 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 420

Query: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVIT 480
           QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYY EYMNTGPGASTANRVNWKGY VIT
Sbjct: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 480

Query: 481 SASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SA+EASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of CSPI03G09730 vs. ExPASy TrEMBL
Match: A0A5D3D1Y4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003750 PE=3 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 7.9e-283
Identity = 485/511 (94.91%), Postives = 502/511 (98.24%), Query Frame = 0

Query: 1   MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKK-TPITT 60
           MARH+IVFL+VL+VLIFP D+LCYSEKEIKNWCSQTPYPAPCEEFLKTKA+TKK TPITT
Sbjct: 1   MARHSIVFLIVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITT 60

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARC 120
           KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYD+IITRLNRTS RC
Sbjct: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRC 120

Query: 121 SPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180
           +PADAQTWLSAALTALETCRTGF+ELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE
Sbjct: 121 TPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180

Query: 181 PTT-MTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 240
           PTT M DGFPTWV PG+RKLLQSGSPKA+VVVAQDGSGNFKTVK+A++AAK GGRFVIY+
Sbjct: 181 PTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKSGGRFVIYV 240

Query: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300
           KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300

Query: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360

Query: 361 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWR 420
           AVVLQDCNIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCRITS+GGLSGVKAYLGRPWR
Sbjct: 361 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 420

Query: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVIT 480
           QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYY EYMNTGPGASTANRVNWKGY VIT
Sbjct: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 480

Query: 481 SASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SA+EASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of CSPI03G09730 vs. ExPASy TrEMBL
Match: A0A6J1E9U7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111432099 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 6.1e-251
Identity = 426/506 (84.19%), Postives = 469/506 (92.69%), Query Frame = 0

Query: 4   HTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHF 63
           H+   L+VL+VL FP+ I  YSEKE+KNWCSQTPYPAPCE+F+K KAT K +PI TKSHF
Sbjct: 5   HSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHF 64

Query: 64  FEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPAD 123
            ++LVETAL+ AVSA K ALSLGPKC N +EK+A TDCVDLY+QII+RLNR++ RCS  D
Sbjct: 65  LKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSVD 124

Query: 124 AQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTM 183
           AQTWLSA+LTAL+TCR   +ELG+SAFGYPLT+NNVSKLISDGLSVNKPASP+G+EP TM
Sbjct: 125 AQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPASPQGFEP-TM 184

Query: 184 TDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVY 243
            +GFPTWV PG+RKLLQSGSP ADVVVA+DGSGNFKTVK+A++AAKGGGRFVIY+K+GVY
Sbjct: 185 AEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVY 244

Query: 244 NENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAG 303
           +ENL+I AKNVM++GDGIGKT+ITGSRSVGGG TTFRSATVAVDGDGFIARDITFRNTAG
Sbjct: 245 SENLEINAKNVMLIGDGIGKTVITGSRSVGGGFTTFRSATVAVDGDGFIARDITFRNTAG 304

Query: 304 AKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQ 363
           A  HQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQ
Sbjct: 305 AAKHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQ 364

Query: 364 DCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQYSRT 423
           DCNIIARDPPN+TIT+TAQGRSDPNQNTGISIHN RITS+GGLSGVKAYLGRPWRQYSRT
Sbjct: 365 DCNIIARDPPNRTITVTAQGRSDPNQNTGISIHNSRITSAGGLSGVKAYLGRPWRQYSRT 424

Query: 424 VVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSASEA 483
           V+MKSSIGGFISPAGW+ WSGNFALNTLYY EYMNTGPGASTANRV WKGY VITSA+EA
Sbjct: 425 VIMKSSIGGFISPAGWLEWSGNFALNTLYYGEYMNTGPGASTANRVKWKGYRVITSATEA 484

Query: 484 SKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 485 SKFTVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of CSPI03G09730 vs. NCBI nr
Match: XP_004133929.1 (pectinesterase 2 [Cucumis sativus] >KGN56617.1 hypothetical protein Csa_010246 [Cucumis sativus])

HSP 1 Score: 1021.5 bits (2640), Expect = 2.4e-294
Identity = 507/509 (99.61%), Postives = 507/509 (99.61%), Query Frame = 0

Query: 1   MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60
           MARHTIVF VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK
Sbjct: 1   MARHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60

Query: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS 120
           SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS
Sbjct: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCS 120

Query: 121 PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP 180
           PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP
Sbjct: 121 PADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEP 180

Query: 181 TTMTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS 240
           TTMTDGFPTWVSPGNRKLLQS SPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS
Sbjct: 181 TTMTDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKS 240

Query: 241 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 300
           GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN
Sbjct: 241 GVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRN 300

Query: 301 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 360
           TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV
Sbjct: 301 TAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAV 360

Query: 361 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY 420
           VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY
Sbjct: 361 VLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQY 420

Query: 421 SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA 480
           SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA
Sbjct: 421 SRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSA 480

Query: 481 SEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SEASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of CSPI03G09730 vs. NCBI nr
Match: XP_008438193.1 (PREDICTED: pectinesterase 2 [Cucumis melo] >KAA0049055.1 pectinesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 984.9 bits (2545), Expect = 2.5e-283
Identity = 487/511 (95.30%), Postives = 503/511 (98.43%), Query Frame = 0

Query: 1   MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKK-TPITT 60
           MARH+IVFLVVL+VLIFP D+LCYSEKEIKNWCSQTPYPAPCEEFLKTKA+TKK TPITT
Sbjct: 1   MARHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITT 60

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARC 120
           KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYD+IITRLNRTS RC
Sbjct: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRC 120

Query: 121 SPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180
           +PADAQTWLSAALTALETCRTGF+ELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE
Sbjct: 121 TPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180

Query: 181 PTT-MTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 240
           PTT M DGFPTWV PG+RKLLQSGSPKA+VVVAQDGSGNFKTVK+A++AAKGGGRFVIY+
Sbjct: 181 PTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 240

Query: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300
           KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300

Query: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360

Query: 361 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWR 420
           AVVLQDCNIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCRITS+GGLSGVKAYLGRPWR
Sbjct: 361 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 420

Query: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVIT 480
           QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYY EYMNTGPGASTANRVNWKGY VIT
Sbjct: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 480

Query: 481 SASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SA+EASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of CSPI03G09730 vs. NCBI nr
Match: TYK17508.1 (pectinesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 982.2 bits (2538), Expect = 1.6e-282
Identity = 485/511 (94.91%), Postives = 502/511 (98.24%), Query Frame = 0

Query: 1   MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKK-TPITT 60
           MARH+IVFL+VL+VLIFP D+LCYSEKEIKNWCSQTPYPAPCEEFLKTKA+TKK TPITT
Sbjct: 1   MARHSIVFLIVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITT 60

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARC 120
           KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYD+IITRLNRTS RC
Sbjct: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRC 120

Query: 121 SPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180
           +PADAQTWLSAALTALETCRTGF+ELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE
Sbjct: 121 TPADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYE 180

Query: 181 PTT-MTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYI 240
           PTT M DGFPTWV PG+RKLLQSGSPKA+VVVAQDGSGNFKTVK+A++AAK GGRFVIY+
Sbjct: 181 PTTIMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKSGGRFVIYV 240

Query: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300
           KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 241 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 300

Query: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 301 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 360

Query: 361 AVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWR 420
           AVVLQDCNIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCRITS+GGLSGVKAYLGRPWR
Sbjct: 361 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 420

Query: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVIT 480
           QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYY EYMNTGPGASTANRVNWKGY VIT
Sbjct: 421 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 480

Query: 481 SASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SA+EASKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 481 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of CSPI03G09730 vs. NCBI nr
Match: XP_038877776.1 (pectinesterase 2 [Benincasa hispida])

HSP 1 Score: 939.1 bits (2426), Expect = 1.6e-269
Identity = 457/503 (90.85%), Postives = 485/503 (96.42%), Query Frame = 0

Query: 7   VFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHFFEI 66
           V +VV+IVLIFP DI CYSE EIKNWCSQTPYPAPCEEFLKTKATTK++PI TKSHFFEI
Sbjct: 7   VLVVVVIVLIFPLDIYCYSEIEIKNWCSQTPYPAPCEEFLKTKATTKRSPIATKSHFFEI 66

Query: 67  LVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPADAQT 126
           LVETALERAVSA +NALSLGPKCRNSKE+TAWTDCVDLYDQIITRLNRT  RC+P DAQT
Sbjct: 67  LVETALERAVSARQNALSLGPKCRNSKERTAWTDCVDLYDQIITRLNRTVDRCTPVDAQT 126

Query: 127 WLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDG 186
           WLSAALTALETCRTG EELG+SAFGYPLTANNVSKLISDGLSVNKPASPEG+EP TM DG
Sbjct: 127 WLSAALTALETCRTGLEELGVSAFGYPLTANNVSKLISDGLSVNKPASPEGFEPPTMEDG 186

Query: 187 FPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVYNEN 246
           FPTWV PG+R+LLQSGSPKA+VVVAQDGSGNFKTVK+A++AAKGGGRFVIY+K+GVY+EN
Sbjct: 187 FPTWVRPGDRRLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSEN 246

Query: 247 LDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKN 306
           ++I AKNVM++GDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKN
Sbjct: 247 VEINAKNVMLIGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKN 306

Query: 307 HQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCN 366
           HQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNAAVV+QDCN
Sbjct: 307 HQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDCN 366

Query: 367 IIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQYSRTVVM 426
           IIARDPPNKTIT+TAQGRSDPNQNTGISIHNCRITS+GGLSGVKAYLGRPWRQYSRTVVM
Sbjct: 367 IIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWRQYSRTVVM 426

Query: 427 KSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSASEASKF 486
           KSSIGGFISPAGWM WSGNFALNTLYY EYMNTGPGASTANRV WKGY VITSA+EASKF
Sbjct: 427 KSSIGGFISPAGWMEWSGNFALNTLYYGEYMNTGPGASTANRVKWKGYRVITSAAEASKF 486

Query: 487 TVGNFIAGGSWLPSTGVPFTSGL 510
           TVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 487 TVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of CSPI03G09730 vs. NCBI nr
Match: XP_022924664.1 (pectinesterase 2-like [Cucurbita moschata] >KAG6582550.1 Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018932.1 Pectinesterase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 876.3 bits (2263), Expect = 1.3e-250
Identity = 426/506 (84.19%), Postives = 469/506 (92.69%), Query Frame = 0

Query: 4   HTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHF 63
           H+   L+VL+VL FP+ I  YSEKE+KNWCSQTPYPAPCE+F+K KAT K +PI TKSHF
Sbjct: 5   HSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHF 64

Query: 64  FEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPAD 123
            ++LVETAL+ AVSA K ALSLGPKC N +EK+A TDCVDLY+QII+RLNR++ RCS  D
Sbjct: 65  LKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSVD 124

Query: 124 AQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTM 183
           AQTWLSA+LTAL+TCR   +ELG+SAFGYPLT+NNVSKLISDGLSVNKPASP+G+EP TM
Sbjct: 125 AQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPASPQGFEP-TM 184

Query: 184 TDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVY 243
            +GFPTWV PG+RKLLQSGSP ADVVVA+DGSGNFKTVK+A++AAKGGGRFVIY+K+GVY
Sbjct: 185 AEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVY 244

Query: 244 NENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAG 303
           +ENL+I AKNVM++GDGIGKT+ITGSRSVGGG TTFRSATVAVDGDGFIARDITFRNTAG
Sbjct: 245 SENLEINAKNVMLIGDGIGKTVITGSRSVGGGFTTFRSATVAVDGDGFIARDITFRNTAG 304

Query: 304 AKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQ 363
           A  HQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQ
Sbjct: 305 AAKHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQ 364

Query: 364 DCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQYSRT 423
           DCNIIARDPPN+TIT+TAQGRSDPNQNTGISIHN RITS+GGLSGVKAYLGRPWRQYSRT
Sbjct: 365 DCNIIARDPPNRTITVTAQGRSDPNQNTGISIHNSRITSAGGLSGVKAYLGRPWRQYSRT 424

Query: 424 VVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSASEA 483
           V+MKSSIGGFISPAGW+ WSGNFALNTLYY EYMNTGPGASTANRV WKGY VITSA+EA
Sbjct: 425 VIMKSSIGGFISPAGWLEWSGNFALNTLYYGEYMNTGPGASTANRVKWKGYRVITSATEA 484

Query: 484 SKFTVGNFIAGGSWLPSTGVPFTSGL 510
           SKFTVGNFIAGGSWLPSTGVPFTSGL
Sbjct: 485 SKFTVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of CSPI03G09730 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 642.1 bits (1655), Expect = 3.7e-184
Identity = 320/516 (62.02%), Postives = 395/516 (76.55%), Query Frame = 0

Query: 1   MARHTIVFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTK 60
           MA    +   V++ ++  S +  Y++K++K WCSQTP P PCE FL   +  +  PI ++
Sbjct: 2   MAFRAYIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNE--PIKSE 61

Query: 61  SHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---SA 120
           S F +I ++  L+RA+ A  +A +LGPKCR+++EK AW DC+ LYD  ++++N T   + 
Sbjct: 62  SEFLKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNV 121

Query: 121 RCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEG 180
           +CS  DAQTWLS ALT L+TCR GF ELG++    PL +NNVS L+ + L++NK   P  
Sbjct: 122 KCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINK--VPFN 181

Query: 181 YEPTTMTDGFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIY 240
           Y P    DGFP+WV PG+RKLLQS +PK + VVA+DGSGNFKT+K+AI AA G GRFVIY
Sbjct: 182 YTPPE-KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIY 241

Query: 241 IKSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDIT 300
           +K GVY+ENL+I+ KNVM+ GDGIGKTIITGS+SVGGG+TTF SATVA  GDGFIAR IT
Sbjct: 242 VKQGVYSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGIT 301

Query: 301 FRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGN 360
           FRNTAGA N QAVALRSGSDLSVFY+C FE YQDTLYV++ RQFY+ CD+YGTVDFIFGN
Sbjct: 302 FRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGN 361

Query: 361 AAVVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGV----KAYL 420
           AA VLQ+CNI AR P +KT T+TAQGRSDPNQNTGI IHN R+T++  L  V    K YL
Sbjct: 362 AAAVLQNCNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYL 421

Query: 421 GRPWRQYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKG 480
           GRPWRQYSRTV MK+S+   I P GW+ W GNFAL TL+YAE+ NTGPGAST+ RV W G
Sbjct: 422 GRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPG 481

Query: 481 YHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           + V+ SASEASKFTVG F+AGGSW+PS+ VPFTSGL
Sbjct: 482 FRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of CSPI03G09730 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 481.1 bits (1237), Expect = 1.1e-135
Identity = 263/536 (49.07%), Postives = 357/536 (66.60%), Query Frame = 0

Query: 1   MARHTIVFL-VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITT 60
           M + +I+FL ++L+VL+    +   +++   +WC +TPYP PC+ + K     ++   T 
Sbjct: 5   MQKLSILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQP--TQ 64

Query: 61  KSHFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRT---- 120
            S F  +LVE A++RA+SA     + G  C +SK++    DC+DLY   I +LNRT    
Sbjct: 65  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 124

Query: 121 ------SARCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTAN-NVSKLISDGLS 180
                 +  C+  DAQTWLS ALT  ETCR G  +L ++ F  P+ +N  +S LIS+ L+
Sbjct: 125 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLA 184

Query: 181 VNKPASPEGYEPTTMTD--GFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAIS 240
           VN      G +  T  +  GFPTW+S  +++LL+  + +A++VVA+DGSG+F TV+ AI 
Sbjct: 185 VNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAID 244

Query: 241 AA----KGGGRFVIYIKSGVYNENLDIKAKN--VMMVGDGIGKTIITGSRSVGGGSTTFR 300
            A       GRFVIY+K G+Y EN++++  N  +M+VGDG+  TIITG RSV GG TT+ 
Sbjct: 245 VAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYN 304

Query: 301 SATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQ 360
           SAT  ++G  FIA+ ITFRNTAG    QAVALRS SDLS+FY+C  EGYQDTL V+++RQ
Sbjct: 305 SATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQ 364

Query: 361 FYKQCDIYGTVDFIFGNAAVVLQDCNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCR 420
           FY++C IYGTVDFIFGNAA V Q+C I+ R P   +   +TAQGR+DP QNTGISIHN R
Sbjct: 365 FYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSR 424

Query: 421 ITSSGGL----SGVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWMPW--SGNFALNTLYY 480
           I  +  L      VK Y+GRPW ++SRTVV+++ +   +SP GW PW     F L+TL+Y
Sbjct: 425 ILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFY 484

Query: 481 AEYMNTGPGASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
           AEY NTGP +ST  RV+WKG+HV+  AS+AS FTVG FIAG +WLP TG+PFTSGL
Sbjct: 485 AEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of CSPI03G09730 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 473.0 bits (1216), Expect = 3.0e-133
Identity = 264/528 (50.00%), Postives = 343/528 (64.96%), Query Frame = 0

Query: 7   VFLVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEE-FLKTKATTKKTPITTKSHFFE 66
           + L++L+ L   + +   +   I  WC +TPYP PC+  F+K       T I   S F  
Sbjct: 16  LLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQI---SEFRV 75

Query: 67  ILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNR--------TSA 126
           +LVE A++RAVSA     +    C + K++    DC++LY   + +LNR        T  
Sbjct: 76  LLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGR 135

Query: 127 RCSPADAQTWLSAALTALETCRTGFEELGLSAFGYPLTAN-NVSKLISDGLSVNKPASPE 186
           RC+  DAQTWLS ALT  ETCR G  +L +S F  P+ +N  +S LIS+ L+VN      
Sbjct: 136 RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTA 195

Query: 187 GYEPTTMTD--GFPTWVSPGNRKLLQSGSPKADVVVAQDGSGNFKTVKDAISAA----KG 246
           G   +T  D  GFPTWVS   R+LLQ  S +A++VVA+DGSG+FKTV+ AI  A      
Sbjct: 196 GKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVT 255

Query: 247 GGRFVIYIKSGVYNENLDIKAK--NVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDG 306
            GRFVIY+K G+Y ENL+++    N+M+VGDG+  TIITG RSV GG TT+ SAT  ++G
Sbjct: 256 SGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEG 315

Query: 307 DGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIY 366
             FIA+ I F+NTAG    QAVALRS SDLS+FYRC  EGYQDTL V+++RQFY++C IY
Sbjct: 316 LHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIY 375

Query: 367 GTVDFIFGNAAVVLQDCNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLS 426
           GTVDFIFGNAAVV Q+C I+ R P   +   +TAQGR+D  QNTGISIHN  I  +  L 
Sbjct: 376 GTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLK 435

Query: 427 ----GVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWMPWS--GNFALNTLYYAEYMNTGP 486
                VK Y+GRPW  YSRTVV+K+ I   +SP GW PW+    + L+TL+YAEY N GP
Sbjct: 436 PVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGP 495

Query: 487 GASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPSTGVPFTSGL 510
            +ST  RV WKG+HV++ AS+AS F+VG FIAG +WLP +G+PFTS L
Sbjct: 496 ASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of CSPI03G09730 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 460.7 bits (1184), Expect = 1.5e-129
Identity = 250/508 (49.21%), Postives = 331/508 (65.16%), Query Frame = 0

Query: 32  WCSQTPYPAPCEEFLK--TKATTKKTPITTKSHFFEILVETALERAVSAHKNALSLGPKC 91
           WC++TP+   C  + +  ++      P   +S F  +LV+ AL++AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 92  RNSKEKTAWTDCVDLYDQIITRLNRT----------SARCSPADAQTWLSAALTALETCR 151
            N++ K AW+DCV+L+   + +LNRT            +C+  DAQTWLS A T +ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 152 TGFEELGLSAFGYPLTAN-NVSKLISDGLSVNKPASPEGYEPTTMT--DGFPTWVSPGNR 211
           +G E+L +S F  P+ +N N+S LI + L+VN     +    TT    + FP+WVS   R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 212 KLLQSGS---PKADVVVAQDGSGNFKTVKDAISAA---KGGGRFVIYIKSGVYNENLDI- 271
           +LL S S       +VVAQD SG+F++++ AI+ A   +   RFVIY+K GVY EN+D+ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 272 -KAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQ 331
               N+M+VGDG  KTIIT  RSV  G TT+ SAT    G  F+A+D+TF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 332 AVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNII 391
           AVA+RS SDLSVFYR G  G+QDTLY++++RQF+++C I GT+DFIFGNAAVV Q+C I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 392 ARDP-PNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGV----KAYLGRPWRQYSRT 451
            R P   +   +TAQGR DP QNTGI+IH+ RI ++  L  V    K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 452 VVMKSSIGGFISPAGWMPW--SGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSAS 510
            +MK+ I   ISP GW PW    NFALNT++Y EY N GPG+ST  RV WKG+H ITS +
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

BLAST of CSPI03G09730 vs. TAIR 10
Match: AT4G00190.1 (pectin methylesterase 38 )

HSP 1 Score: 456.4 bits (1173), Expect = 2.9e-128
Identity = 244/489 (49.90%), Postives = 319/489 (65.24%), Query Frame = 0

Query: 33  CSQTPYPAPCEEFLKTKATTKKTPITTKSHFFEILVETALERAVSAHKNALSLGPKCRNS 92
           C +TP+P  C+  L      K  PI + + F ++ VE  L+ AV A  +   L P+  + 
Sbjct: 8   CDETPHPGECKTLL-----IKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS- 67

Query: 93  KEKTAWTDCVDLYDQIITRLNRT----SARCSPADAQTWLSAALTALETCRTGFEELGLS 152
             K AW +C+DLY+Q I RLN +       CS +D Q WLS ALT L+TC+    ELG+S
Sbjct: 68  --KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGVS 127

Query: 153 AFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDGFPTWVSPGNRKLLQSGSPKADV 212
           +     +  +++  + + L++NK     G E           +S    K L  G  K DV
Sbjct: 128 SH----SLESITIDVINTLAINKRMEQNGKE---------FGISKITMKTLSIGE-KVDV 187

Query: 213 VVAQDGSGNFKTVKDAISAA----KGGGRFVIYIKSGVYNE--NLDIKAKNVMMVGDGIG 272
           VVAQDGSG++KT+++A++ A    KG  R+VI++K GVY E  N+ IK+ N+M+ GDGIG
Sbjct: 188 VVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDGIG 247

Query: 273 KTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFY 332
           KTIITG +S G G +T++SAT   +GDGF+ RDIT RNTAG +NHQAVALRS SD+SVFY
Sbjct: 248 KTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALRSNSDMSVFY 307

Query: 333 RCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVLQDCNIIARDPPNKTITLTAQ 392
           RC  EGYQDTLYV++ RQF+++CDIYGTVDFIFGNAA VLQ+C I AR+PPN   T+TAQ
Sbjct: 308 RCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPPNGVNTITAQ 367

Query: 393 GRSDPNQNTGISIHNCRITSSGG--LSGVKAYLGRPWRQYSRTVVMKSSIGGFISPAGWM 452
            R +PNQ TGI IHN  +  + G  L GVK YLGRPWR Y+RTVV+ + +   I P GW+
Sbjct: 368 SRFNPNQTTGIVIHNSVVKGAPGVQLGGVKTYLGRPWRSYARTVVIGTYLDTLIEPNGWI 427

Query: 453 PWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITSASEASKFTVGNFIAGGSWLPS 510
            W    AL+TLYY EY N+GPG+ T NRV+W G+HVI+   EA +FT+  FI   SWLP 
Sbjct: 428 DWDNVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFTLPKFIDSASWLPP 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O048873.5e-18763.55Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221495.3e-18362.02Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN51.6e-13449.07Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN44.2e-13250.00Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3... [more]
Q9STY32.2e-12849.21Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A0A0L7N41.2e-29499.61Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1[more]
A0A5A7U6031.2e-28395.30Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00283... [more]
A0A1S3AVW41.2e-28395.30Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1[more]
A0A5D3D1Y47.9e-28394.91Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00375... [more]
A0A6J1E9U76.1e-25184.19Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111432099 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004133929.12.4e-29499.61pectinesterase 2 [Cucumis sativus] >KGN56617.1 hypothetical protein Csa_010246 [... [more]
XP_008438193.12.5e-28395.30PREDICTED: pectinesterase 2 [Cucumis melo] >KAA0049055.1 pectinesterase 2 [Cucum... [more]
TYK17508.11.6e-28294.91pectinesterase 2 [Cucumis melo var. makuwa][more]
XP_038877776.11.6e-26990.85pectinesterase 2 [Benincasa hispida][more]
XP_022924664.11.3e-25084.19pectinesterase 2-like [Cucurbita moschata] >KAG6582550.1 Pectinesterase 2, parti... [more]
Match NameE-valueIdentityDescription
AT2G45220.13.7e-18462.02Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51490.11.1e-13549.07Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51500.13.0e-13350.00Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G47400.11.5e-12949.21Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G00190.12.9e-12849.90pectin methylesterase 38 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 24..169
e-value: 2.0E-32
score: 123.7
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 3..149
e-value: 2.7E-26
score: 90.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 28..165
e-value: 1.5E-24
score: 87.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 202..509
e-value: 2.0E-121
score: 407.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 23..171
e-value: 2.2E-25
score: 91.4
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 27..170
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 207..494
e-value: 1.8E-136
score: 453.7
NoneNo IPR availablePANTHERPTHR31707:SF160PECTINESTERASEcoord: 6..508
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 6..508
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 32..174
e-value: 3.21539E-26
score: 102.516
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 230..249
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 347..356
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 204..508

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G09730.1CSPI03G09730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity