CSPI03G09680 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G09680
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptiontRNA N6-adenosine threonylcarbamoyltransferase
LocationChr3: 7846763 .. 7851103 (-)
RNA-Seq ExpressionCSPI03G09680
SyntenyCSPI03G09680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTTTTAAGAACTTTTGTTGCTTTTAGAATTTGGTTAAGAATTCAACTTTTTGGTCTAAATTTATAATGCCACATCAAGGCAGAGCTGCTTGCTCGCTATGGAGGCGTTGCTCCTAAAATGGCAGAAGAAGCGCACGCACAAGTGATTGACAAGGTTATGATTGCAAGGTATACTAAATACTCCAATTTCAGCATCCTTTTCATCCACTCTTACATTGGCTTCGGTTATTTTTCGTCTGTCAACTTCAGGTGGTGCAGGAAACAATGGAAAAGGCTAACTTAACAGAAAGGGATTTATCTGCAGTTGCTGTGACCATTGGCCCTGGTTTGAGCCTCTGCCTCCGTGGTAATTTTGCAGGATCCCTCCTTATGGTTTAAGTCCTAAGTTGTACTCTGTCTACTATGTGGACATCTTTCCGCTAATTGAAAGCCGTTGTTTTATCTCGTTATCAGTCACTGTTCAACAGACACCTAAGCTGTAGATTTCTGTAAAAAGAAAGGTTTAGATTTATGTTTTAATGCTTATGTGTACAGGAAAATTGTTCTTAGGCTTTTAGTTATAGAAAGACGCACTGTTCTCTACACTACGTATAAGTTCACAATCAATTTGGCTTCTTTTTCTATCTTTAATTATTCAAGTGAAAATTTATTTTTAATTGGAGATCCTTCGGACCCATTCACCATGCAATTGAAATGCAGTTGGAGTGCAGAAGGCACGAAAAATTGCTGGTAGCTCTAGTCTACCAATTGTTGGGGTGCATCACATGGAAGCTCATGCTCTGGTAGCTAGGTATGTATAACAAATTAAAAATGCAAGTTACATCTGGGTCAGCTTATATTTGTTAATCTGTGAAAGATTTTTCAAAGGAAACTATTTGATGGTGTTTACTAGAGGATGAATATTTGATTTCGTTTGTACTTTCTGGTTGGACACTCTACTCTTCTTATTAAAACATTATTCTTCATTCTGTAGATTAGTTGAACGGGAACTGCAATTTCCTTTCATGGCACTGCTCATTTCTGGTAAAAATTTGTATTCTTATTTTCCTTCCCCTGCATTCTTCCTTCATCTTCATTACATAGATACAAGTTGTGTTTACGTTTCCTGTGTCTAGATGTGTCAAAAATAGAAAGGATACAGAGTGATTTTTTAAACTACTTGATTGTTTCCAACTTTTAGTTTCAAAATTTTAGAGACTTTTATAATTTTATACATCTCCAAATCCAATTATGAAATGCTTGCAAGAGGTTATCTTTTCATAGTTGTTAAGATTAGTTCTCTTTTTTGATAGCATTAAATTTAAATGGTCGAGCTCTGTCATTACCATCTCAGCACATTATTTGAACTATTTTCACAATTCTTTAGCAACAGAGCCTACGGTTCTGATTGTTGATTTTTCTATCTGGGTTGAACTTCAACTTTTCATGAATAGAACTTGTGCTGTAATTTTCAATGATTCATGGATGGGACGCTTGAAAAAGACGACAAACTCATACTTTATTTTTAATCAGCCACGATGGAGAGAAAAAGGAAAACTGGATCTTTTTCATGGCTTTAGGGTTAAACTTTGTCCGTGTCATCATATCAGTTTATTCTATTAGCTATTCATTGAGAAGTTAATTATTGGGTCCTCTTGAACGTGTTATTAGGATGACTCAAAATGGTTTCGGTTGCTTGCATTCGAGCTTTTGAGTTTTGACAAAGATTTTAAAGAATGAAAAATTAAATGTCATACATTCTAGGGGGACACAATTTACTTGTTCTCGCACAAGATCTTGGCCAATACATACAACTTGGAACAACAATAGACGATGCCATTGGGGAGGCGTATGACAAGACTGCCAAATGGCTTGGTCTTGATCTGAGGAAGAGTGGTGGGCCGGCTATAGAGGAGCTTGCCAGAGAGGGGGATCCAAAGTCAATAAAGTTTTCTGTAAGTCTTCAAACTCATTATGTTGTTCGTCATTGCATATGCCTGTTTTTATCGTGTAGGTGGTGCATAAACATGAGAAACATTTTATAACCATACATTTCTATGTTAGAGTGTACATTAAGTTCTTCAAATATTTCTTTCTTTAGACTCCAATGAAGCAACATAAAGATTGCAACTTCTCCTATGCGGGGCTGAAGACCCAAGTCAGGCTGGCTATTGAATCCCGAAACATGTGTGTATTTATCTGGAATGTGCATACTTACAATTTTTATAAAGGAAACTGATTTTCTAAATATTTTTTTGTTATCTTATTATCTTATTCATCCTTTCTAATAACAAAATCACCAGCAAGTATAATATACAGAAAAATGAAAATCCATATATGTAAAAAAGGGTCAGGCATAGTTAATATTTATTGTATTGCAAGATTAAAGGTCTATTAGATTTGAAGTTTTGATATCTCTATCGGAATTTAAATAACCCATGGTTTTAATTTCACATGTAATATCTGAGCAATTGGGTTTTCTGTCTTGCGTAGTTTGTTTCCATATCTACAGTTTCGTCTGATTGCTAGGAACTGGGTTGATGGTTATGTCTGAGAAGCTTATTGAAGAGACTTTTGATGGGTTGAAAGTTGCTGTTTTAATTTATTTTCTGAGTCATTTGATGAATGTACATGCCGTGTGCAGTGACACAAAAATTCCTCTGTCGTCTGCAAGCATCCAAGACAGAAGTGTAAGGGCTGATATAGCTGCCTCCTTTCAGGTATCCATCAGTCTGTAACTGTATGGACTAAATTTATATTGTTACATCAACTGATCCATTTGAAATTCATTGCAGCGTGTTGCAGTGCTACATCTAGAGGACAAATGTGAACGAGCAATTGAATGGGCGTTGAAGATTGAACCATCCATAAGACATTTGGTAAAATGAGACGAAAAACTTCTTTTGTTCCTTTATATCTCATGATTCTTTCATCTTACATTTACATTTTCTTGCCAGTGAGCTGCACACATAGTTGAAGTGAGGAATTCTCTTATGTTCTGCCTATGCAGGTTGTCTCAGGAGGTGTTGCATCAAATAAGTATGTTAGGGCTCAACTTATGGCGGTTGTCAGGAAGAAGGGCCTGCAGCTTGTCTGCCCTCCTCCCAGTCTCTGCACTGATAATGGTATAATTTCTTTAGCTCAACTTGATGTTAAATTGTGGATATGTTTGTGTCTACTAAATTAATAAACAGTTCAGAACCTGAACCTGAGACATCAAACTGTAGGTTGACCAATTATCATTTGAAAATAATTGATGCTCGTGATTTCTTTTTGAGCTCCAAGTGTGCTAGTTGATGTGTTTGTGAGAGAATTATAGAGCTTTCCTAAATAAAGTAGCGATTTCTTATGCAGAAGAATCTTAATATACAACGGCTTTGCTGATCATTCCTGGTGCAAGCTTATTTAAATAAATTCATACACTATATGGTATTCATATTTGTTTCACGCCATAGGAACCCATTTACTTTTAATTGTGTTGGCAAAGGTGTAATGGTGGCTTGGACGGGCCTCGAGCATTTTCGCATAGGGAGATTTGATCCTCCTCCACCAGCTGATGAACCTGAAGATACAATGGTATGGTTCTAATCCCTCATCTGTAATCCCTCTGCCCCTTGAATTAAATAGAAACAAGTTAGTTGATGAAGCTATCAAATGTTTGGGTTCTGCCTCTGCTTTTGAGCGTATAAAAAGAATAATACAAGCCCATGGGGTGTCAGGCGAGCTGGTCTCGAGTTAGAACGTTTGCGTCAGTTTGACGTCAGCCCCTTCTGAAGTTATGGATACAATTATGTTGTCCAAAATGATTTGAAGAGCCTGTAGAATATTCTAGTTTATGATGACAGTATCCTCCTTTTATTTTCTGGTTTTTCGATCCTTCTCAAGTTTACATTTTGTTCCCAGTATGACGTGCGACCGAGATGGCCATTAGGAGAAGAATACACCGAGGGAAGGAGTGCAGCTCGCTCACTAAGAACAGCCCGAATCCATCCATCACTTACGTCTATCATCCAAGCATCTCTACAGCAACAACAGTAGCTGTAGCTTCAGTTCCCAAATTCCAACAAAAGGAATTTGCATTCTTTTGCATCTCTTCTTCCATGTGCGACTTCTTAGACAACAAATGGAATCATATCAGGAGCTCGATGAAGCACCCGGGCCATCAATGCGGAACTTCCAATGCTCAATGCCTTCTGTTAACTTATTGGGAGATTTTGGTTGGTTTCATGAGATATGCCATCGATATGTTGCATTATGTAGTGATTCACCTGTATCATACTTACAACTAAGAAGACTCTTATATTTGTTACAGTTTGTATCATATTCTCATTTATAAAAGAAAAACAGAGACCTTTTTTCAC

mRNA sequence

AATTTTTAAGAACTTTTGTTGCTTTTAGAATTTGGTTAAGAATTCAACTTTTTGGTCTAAATTTATAATGCCACATCAAGGCAGAGCTGCTTGCTCGCTATGGAGGCGTTGCTCCTAAAATGGCAGAAGAAGCGCACGCACAAGTGATTGACAAGGTGGTGCAGGAAACAATGGAAAAGGCTAACTTAACAGAAAGGGATTTATCTGCAGTTGCTGTGACCATTGGCCCTGGTTTGAGCCTCTGCCTCCGTGTTGGAGTGCAGAAGGCACGAAAAATTGCTGGTAGCTCTAGTCTACCAATTGTTGGGGTGCATCACATGGAAGCTCATGCTCTGGTAGCTAGATTAGTTGAACGGGAACTGCAATTTCCTTTCATGGCACTGCTCATTTCTGGGGGACACAATTTACTTGTTCTCGCACAAGATCTTGGCCAATACATACAACTTGGAACAACAATAGACGATGCCATTGGGGAGGCGTATGACAAGACTGCCAAATGGCTTGGTCTTGATCTGAGGAAGAGTGGTGGGCCGGCTATAGAGGAGCTTGCCAGAGAGGGGGATCCAAAGTCAATAAAGTTTTCTACTCCAATGAAGCAACATAAAGATTGCAACTTCTCCTATGCGGGGCTGAAGACCCAAGTCAGGCTGGCTATTGAATCCCGAAACATTGACACAAAAATTCCTCTGTCGTCTGCAAGCATCCAAGACAGAAGTGTAAGGGCTGATATAGCTGCCTCCTTTCAGCGTGTTGCAGTGCTACATCTAGAGGACAAATGTGAACGAGCAATTGAATGGGCGTTGAAGATTGAACCATCCATAAGACATTTGGTTGTCTCAGGAGGTGTTGCATCAAATAAGTATGTTAGGGCTCAACTTATGGCGGTTGTCAGGAAGAAGGGCCTGCAGCTTGTCTGCCCTCCTCCCAGTCTCTGCACTGATAATGGTGTAATGGTGGCTTGGACGGGCCTCGAGCATTTTCGCATAGGGAGATTTGATCCTCCTCCACCAGCTGATGAACCTGAAGATACAATGTATGACGTGCGACCGAGATGGCCATTAGGAGAAGAATACACCGAGGGAAGGAGTGCAGCTCGCTCACTAAGAACAGCCCGAATCCATCCATCACTTACGTCTATCATCCAAGCATCTCTACAGCAACAACAGTAGCTGTAGCTTCAGTTCCCAAATTCCAACAAAAGGAATTTGCATTCTTTTGCATCTCTTCTTCCATGTGCGACTTCTTAGACAACAAATGGAATCATATCAGGAGCTCGATGAAGCACCCGGGCCATCAATGCGGAACTTCCAATGCTCAATGCCTTCTGTTAACTTATTGGGAGATTTTGGTTGGTTTCATGAGATATGCCATCGATATGTTGCATTATGTAGTGATTCACCTGTATCATACTTACAACTAAGAAGACTCTTATATTTGTTACAGTTTGTATCATATTCTCATTTATAAAAGAAAAACAGAGACCTTTTTTCAC

Coding sequence (CDS)

ATGGCAGAAGAAGCGCACGCACAAGTGATTGACAAGGTGGTGCAGGAAACAATGGAAAAGGCTAACTTAACAGAAAGGGATTTATCTGCAGTTGCTGTGACCATTGGCCCTGGTTTGAGCCTCTGCCTCCGTGTTGGAGTGCAGAAGGCACGAAAAATTGCTGGTAGCTCTAGTCTACCAATTGTTGGGGTGCATCACATGGAAGCTCATGCTCTGGTAGCTAGATTAGTTGAACGGGAACTGCAATTTCCTTTCATGGCACTGCTCATTTCTGGGGGACACAATTTACTTGTTCTCGCACAAGATCTTGGCCAATACATACAACTTGGAACAACAATAGACGATGCCATTGGGGAGGCGTATGACAAGACTGCCAAATGGCTTGGTCTTGATCTGAGGAAGAGTGGTGGGCCGGCTATAGAGGAGCTTGCCAGAGAGGGGGATCCAAAGTCAATAAAGTTTTCTACTCCAATGAAGCAACATAAAGATTGCAACTTCTCCTATGCGGGGCTGAAGACCCAAGTCAGGCTGGCTATTGAATCCCGAAACATTGACACAAAAATTCCTCTGTCGTCTGCAAGCATCCAAGACAGAAGTGTAAGGGCTGATATAGCTGCCTCCTTTCAGCGTGTTGCAGTGCTACATCTAGAGGACAAATGTGAACGAGCAATTGAATGGGCGTTGAAGATTGAACCATCCATAAGACATTTGGTTGTCTCAGGAGGTGTTGCATCAAATAAGTATGTTAGGGCTCAACTTATGGCGGTTGTCAGGAAGAAGGGCCTGCAGCTTGTCTGCCCTCCTCCCAGTCTCTGCACTGATAATGGTGTAATGGTGGCTTGGACGGGCCTCGAGCATTTTCGCATAGGGAGATTTGATCCTCCTCCACCAGCTGATGAACCTGAAGATACAATGTATGACGTGCGACCGAGATGGCCATTAGGAGAAGAATACACCGAGGGAAGGAGTGCAGCTCGCTCACTAAGAACAGCCCGAATCCATCCATCACTTACGTCTATCATCCAAGCATCTCTACAGCAACAACAGTAG

Protein sequence

MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEPEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ*
Homology
[Close]
BLAST of CSPI03G09680 vs. ExPASy Swiss-Prot
Match: O22145 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GCP1 PE=2 SV=2)

HSP 1 Score: 574.7 bits (1480), Expect = 7.1e-163
Identity = 280/348 (80.46%), Postives = 320/348 (91.95%), Query Frame = 0 

Query: 2   AEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPI 61
           AEEAH++VIDKVVQ+ ++KANLTE+DLSAVAVTIGPGLSLCLRVGV+KAR++AG+ SLPI
Sbjct: 130 AEEAHSRVIDKVVQDALDKANLTEKDLSAVAVTIGPGLSLCLRVGVRKARRVAGNFSLPI 189

Query: 62  VGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAY 121
           VGVHHMEAHALVARLVE+EL FPFMALLISGGHNLLVLA  LGQY QLGTT+DDAIGEA+
Sbjct: 190 VGVHHMEAHALVARLVEQELSFPFMALLISGGHNLLVLAHKLGQYTQLGTTVDDAIGEAF 249

Query: 122 DKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIES 181
           DKTAKWLGLD+ +SGGPA+EELA EGD KS+KF+ PMK HKDCNFSYAGLKTQVRLAIE+
Sbjct: 250 DKTAKWLGLDMHRSGGPAVEELALEGDAKSVKFNVPMKYHKDCNFSYAGLKTQVRLAIEA 309

Query: 182 RNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSG 241
           + ID K P+SSA+ +DR  RADIAASFQRVAVLHLE+KCERAI+WAL++EPSI+H+V+SG
Sbjct: 310 KEIDAKCPVSSATNEDRRNRADIAASFQRVAVLHLEEKCERAIDWALELEPSIKHMVISG 369

Query: 242 GVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEP 301
           GVASNKYVR +L  +V  K L+LVCPPPSLCTDNGVMVAWTGLEHFR+GR+DPPPPA EP
Sbjct: 370 GVASNKYVRLRLNNIVENKNLKLVCPPPSLCTDNGVMVAWTGLEHFRVGRYDPPPPATEP 429

Query: 302 EDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQA-SLQQQ 349
           ED +YD+RPRWPLGEEY +GRS ARS+RTARIHPSLTSII+A SLQQQ
Sbjct: 430 EDYVYDLRPRWPLGEEYAKGRSEARSMRTARIHPSLTSIIRADSLQQQ 477

[Close]
BLAST of CSPI03G09680 vs. ExPASy Swiss-Prot
Match: B3PQA4 (tRNA N6-adenosine threonylcarbamoyltransferase OS=Rhizobium etli (strain CIAT 652) OX=491916 GN=tsaD PE=3 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 3.3e-59
Identity = 134/317 (42.27%), Postives = 188/317 (59.31%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           +A  AH + +D++++E + +AN++  D+ A+A T GPGL   L VG+   + IA ++  P
Sbjct: 54  IAARAHVEALDELIEEALMRANVSLADVDAIAATSGPGLIGGLLVGLMTGKAIAKAAGKP 113

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           +  V+H+E HAL ARL +  L FP++ LL+SGGH  L+L + +G Y + GTTIDDA+GEA
Sbjct: 114 LYAVNHLEGHALTARLTD-GLSFPYLMLLVSGGHTQLILVRGVGDYERWGTTIDDALGEA 173

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           +DKTAK LGL     GGPA+E +AR+G+     F  P+      +FS++GLKT VRLA +
Sbjct: 174 FDKTAKLLGLPY--PGGPAVERMARDGNAGRFDFPRPLVGEARLDFSFSGLKTAVRLAAQ 233

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRH---L 240
                       A + D+ V ADI ASFQR     L+D+  R ++   +  PSI     L
Sbjct: 234 ----------EIAPLSDQDV-ADICASFQRAISRTLKDRIGRGLQRFKREFPSIGETPAL 293

Query: 241 VVSGGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPP 300
           VV+GGVA+N  +RA L  +  K G + + PP SLCTDN VM+AW GLE   IG       
Sbjct: 294 VVAGGVAANLELRATLQGLCDKNGFRFIAPPLSLCTDNAVMIAWAGLERMAIG------- 348

Query: 301 ADEPEDTMYDVRPRWPL 315
              P+      R RWPL
Sbjct: 354 -VAPDALDVQPRSRWPL 348

[Close]
BLAST of CSPI03G09680 vs. ExPASy Swiss-Prot
Match: Q2VYV2 (tRNA N6-adenosine threonylcarbamoyltransferase OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=tsaD PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 7.3e-59
Identity = 133/324 (41.05%), Postives = 182/324 (56.17%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           +A  +H   +DK+V E M +A +   DL AVA T GPGL   + VGV   + IA ++  P
Sbjct: 46  IAARSHLAHMDKLVAEAMRRAGVGFADLDAVAATGGPGLIGGVIVGVMTGKAIALAAGKP 105

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
            + V+H+E HAL  RL   ++ FP++ LL SGGH  L+  + +G+Y +LGTTIDDA GEA
Sbjct: 106 FLAVNHLEGHALTPRLT-HDIAFPYLLLLASGGHCQLLAVEGVGRYTRLGTTIDDAAGEA 165

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           +DK AK  GL     GGPA+E  A+ GDP       PMK    C+FS++GLK   RL IE
Sbjct: 166 FDKVAKMAGLGY--PGGPAVEAAAQGGDPARFTLPRPMKGKPGCDFSFSGLKNAARLLIE 225

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           S       PLS+A       +AD+A +FQ      + D+  R +       P  +HLVV+
Sbjct: 226 S----LPQPLSAAD------QADVARAFQDAVADAMADRVRRGVREMKSRWPQTQHLVVA 285

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVA+N  +R  L+ +  + GL+ + PP  LCTDN  M+AW GLE  R+G+ D       
Sbjct: 286 GGVAANTALRQVLVRIGNETGLEFLAPPLKLCTDNAAMIAWAGLERLRLGQSD------- 345

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSA 325
             D  +  RPRWPL     +G  A
Sbjct: 346 --DLTFAPRPRWPLDPTARKGAKA 347

[Close]
BLAST of CSPI03G09680 vs. ExPASy Swiss-Prot
Match: Q0P4K0 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Xenopus tropicalis OX=8364 GN=osgepl1 PE=2 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 125/298 (41.95%), Postives = 177/298 (59.40%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           +A+  H   I KVV + +  + ++  +LSA+A T+ PGL LCL VG+  +  +      P
Sbjct: 73  VAQHLHRDNITKVVNKAIHASGISPYELSAIATTVKPGLGLCLGVGLSYSLDLVNKYHKP 132

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
            + +HHMEAHAL  RL+   ++FPF+ LLISGGH +L +   + +++ LG ++D+A G+ 
Sbjct: 133 FIPIHHMEAHALTVRLL-HPVEFPFLVLLISGGHCILAIVSGVSEFVMLGHSLDEAPGDT 192

Query: 121 YDKTAKWLGLDLR-----KSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQV 180
            DK A+ L L         SGG AIE LA  G+ K  K   PM  H+DCNFS+AGL+ QV
Sbjct: 193 LDKVARRLSLINHPQCSGMSGGEAIEHLALHGNRKICKLKIPMSHHRDCNFSFAGLRNQV 252

Query: 181 RLAIESRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALK--IEPS 240
              IE +  +  I    +  Q     ADIAA+ Q    LHL  + +RAI +  +  + P+
Sbjct: 253 NKVIEQKEAEKGI----SKGQLLPCVADIAAAVQHTVALHLAQRTQRAIYYCKREGLIPT 312

Query: 241 IRH-LVVSGGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIG 291
            R  LVVSGGVASN+Y+R  L  V  +  + L+CPPP LCTDNGVM+AW G+E  + G
Sbjct: 313 ERACLVVSGGVASNRYIRKALQTVTHESDMTLLCPPPRLCTDNGVMIAWNGIEKLQSG 365

[Close]
BLAST of CSPI03G09680 vs. ExPASy Swiss-Prot
Match: Q1MAQ8 (tRNA N6-adenosine threonylcarbamoyltransferase OS=Rhizobium leguminosarum bv. viciae (strain 3841) OX=216596 GN=tsaD PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.6e-58
Identity = 129/317 (40.69%), Postives = 186/317 (58.68%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           +A  AH + +D++++E +++AN++  D+ A+A T GPGL   L VG+   + IA ++  P
Sbjct: 54  IAARAHVEALDELIEEALKRANVSLNDVDAIAATSGPGLIGGLLVGLMTGKAIARAAGKP 113

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           +  ++H+E HAL ARL +  L FP++ LL+SGGH  L+L + +GQY + GTTIDDA+GEA
Sbjct: 114 LYAINHLEGHALTARLTD-GLSFPYLMLLVSGGHTQLILVRGVGQYERWGTTIDDALGEA 173

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           +DKTAK LGL     GGPA+E +AR+G+P    F  P+      +FS++GLKT VR A +
Sbjct: 174 FDKTAKLLGLPY--PGGPAVERMARDGNPDRFDFPRPLVGEARLDFSFSGLKTAVRQAAQ 233

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRH---L 240
                       A + D+ V ADI ASFQ+     L+D+  R ++      P+      L
Sbjct: 234 ----------DIAPLSDQDV-ADICASFQKAVSRTLKDRIGRGLQRFKTEFPATGEKPAL 293

Query: 241 VVSGGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPP 300
           VV+GGVA+N  +R  L A+  K G + + PP  LCTDN VM+AW GLE    G       
Sbjct: 294 VVAGGVAANLELRGTLQALCNKNGFRFIAPPLHLCTDNAVMIAWAGLERMATGA------ 348

Query: 301 ADEPEDTMYDVRPRWPL 315
              P+      R RWPL
Sbjct: 354 --APDTLDVQPRSRWPL 348

[Close]
BLAST of CSPI03G09680 vs. ExPASy TrEMBL
Match: A0A0A0L938 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumis sativus OX=3659 GN=GCP1 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 4.7e-194
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP
Sbjct: 138 MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 197

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 198 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 257

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE
Sbjct: 258 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 317

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS
Sbjct: 318 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 377

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE
Sbjct: 378 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 437

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 350
           PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 438 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 486

[Close]
BLAST of CSPI03G09680 vs. ExPASy TrEMBL
Match: A0A1S3AWD3 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103483365 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 6.8e-193
Identity = 347/349 (99.43%), Postives = 347/349 (99.43%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP
Sbjct: 117 MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 176

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 177 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 236

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE
Sbjct: 237 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 296

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS
Sbjct: 297 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 356

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKYVRAQL AV RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE
Sbjct: 357 GGVASNKYVRAQLTAVARKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 416

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 350
           PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 417 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 465

[Close]
BLAST of CSPI03G09680 vs. ExPASy TrEMBL
Match: A0A6J1EFN5 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111432100 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.3e-185
Identity = 332/348 (95.40%), Postives = 339/348 (97.41%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           MAEEAHAQ IDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGS SLP
Sbjct: 119 MAEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLP 178

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 179 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 238

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRLAIE
Sbjct: 239 YDKTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 298

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNID KIPLSSAS QDRSVRADIAASFQRVAV HLE++CERAIEWALKIEPSI+HLVVS
Sbjct: 299 SRNIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVS 358

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKYVR+QL  VVRKKGLQLVCPPPSLCTDNGVMVAWTGLEH+RIGRFDPPPPADE
Sbjct: 359 GGVASNKYVRSQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADE 418

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQ 349
           PEDT+YDVRPRWPLGEEY EGRS ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 419 PEDTLYDVRPRWPLGEEYVEGRSVARSLRTARIHPSLTSIIQASLQQQ 466

[Close]
BLAST of CSPI03G09680 vs. ExPASy TrEMBL
Match: A0A6J1IT72 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111479704 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 8.9e-185
Identity = 331/349 (94.84%), Postives = 340/349 (97.42%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           +AEEAHAQ IDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARK+AGS SLP
Sbjct: 119 IAEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKMAGSFSLP 178

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 179 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 238

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRLAIE
Sbjct: 239 YDKTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 298

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNID KIPLSSAS QDRSVRADIAASFQRVAV HLE++CERAIEWALKIEPSI+HLVVS
Sbjct: 299 SRNIDGKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVS 358

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKYVR+QL  VVRKKGLQLVCPPPSLCTDNGVMVAWTGLEH+RIGRFDPPPPADE
Sbjct: 359 GGVASNKYVRSQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADE 418

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 350
           PEDTMYDVRPRWPLGEEY EGRS ARSLRTARIHPSLTSIIQASLQQ+Q
Sbjct: 419 PEDTMYDVRPRWPLGEEYVEGRSVARSLRTARIHPSLTSIIQASLQQRQ 467

[Close]
BLAST of CSPI03G09680 vs. ExPASy TrEMBL
Match: A0A6J1D0B8 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016118 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 9.9e-184
Identity = 326/349 (93.41%), Postives = 338/349 (96.85%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKAR+IAGS SLP
Sbjct: 121 MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARRIAGSYSLP 180

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 181 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 240

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLR+SGGPAIEELAREGDP+S KFSTPMKQHKDCNFSYAGLKTQVRLAIE
Sbjct: 241 YDKTAKWLGLDLRRSGGPAIEELAREGDPQSFKFSTPMKQHKDCNFSYAGLKTQVRLAIE 300

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNID K+P+SS S QDRS+RADIAASFQRVAVLHLE++CERAIEWALKIEPSIRHLVVS
Sbjct: 301 SRNIDAKVPISSESSQDRSLRADIAASFQRVAVLHLEERCERAIEWALKIEPSIRHLVVS 360

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKY+R +L  VVRKKGLQLVCPPPSLCTDNGVMVAWTGLEH+ +GRFDPPPP DE
Sbjct: 361 GGVASNKYIRTRLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYCVGRFDPPPPVDE 420

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 350
           PEDTMYDVRPRWPLGEEYTEGRS ARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 421 PEDTMYDVRPRWPLGEEYTEGRSVARSLRTARIHPSLTSIIQASLQQQQ 469

[Close]
BLAST of CSPI03G09680 vs. NCBI nr
Match: XP_011650793.2 (probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucumis sativus] >KGN56611.1 hypothetical protein Csa_011396 [Cucumis sativus])

HSP 1 Score: 686.8 bits (1771), Expect = 9.8e-194
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP
Sbjct: 138 MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 197

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 198 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 257

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE
Sbjct: 258 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 317

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS
Sbjct: 318 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 377

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE
Sbjct: 378 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 437

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 350
           PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 438 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 486

[Close]
BLAST of CSPI03G09680 vs. NCBI nr
Match: XP_008438179.1 (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucumis melo])

HSP 1 Score: 682.9 bits (1761), Expect = 1.4e-192
Identity = 347/349 (99.43%), Postives = 347/349 (99.43%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP
Sbjct: 117 MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 176

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 177 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 236

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE
Sbjct: 237 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 296

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS
Sbjct: 297 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 356

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKYVRAQL AV RKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE
Sbjct: 357 GGVASNKYVRAQLTAVARKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 416

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 350
           PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 417 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 465

[Close]
BLAST of CSPI03G09680 vs. NCBI nr
Match: XP_038877779.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Benincasa hispida])

HSP 1 Score: 671.8 bits (1732), Expect = 3.2e-189
Identity = 338/349 (96.85%), Postives = 345/349 (98.85%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP
Sbjct: 119 MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 178

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 179 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 238

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRLAIE
Sbjct: 239 YDKTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 298

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNID KIPLSSASIQDRSVRADIAASFQR+AVLHLE++CERAI+WALKIEPSIRHLVVS
Sbjct: 299 SRNIDAKIPLSSASIQDRSVRADIAASFQRIAVLHLEERCERAIDWALKIEPSIRHLVVS 358

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKYVRAQL AVVRKKGLQLVCPPPSLCTDNGVMVAWTG+EHFR+GRFDPPPPADE
Sbjct: 359 GGVASNKYVRAQLTAVVRKKGLQLVCPPPSLCTDNGVMVAWTGIEHFRVGRFDPPPPADE 418

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQQ 350
           PED MYDVRPRWPLGEEYTEGRS ARSLRTARIHPSLTSIIQASLQQQQ
Sbjct: 419 PEDIMYDVRPRWPLGEEYTEGRSVARSLRTARIHPSLTSIIQASLQQQQ 467

[Close]
BLAST of CSPI03G09680 vs. NCBI nr
Match: XP_023527336.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658.7 bits (1698), Expect = 2.8e-185
Identity = 333/348 (95.69%), Postives = 338/348 (97.13%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           MAEEAHAQ IDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGS SLP
Sbjct: 119 MAEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLP 178

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 179 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 238

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRL IE
Sbjct: 239 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIE 298

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNID KIPLSSAS QDRSVRADIAASFQRVAV HLE++CERAIEWALKIEPSI+HLVVS
Sbjct: 299 SRNIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVS 358

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKYVRAQL  VVRKKGLQLVCPPPSLCTDNGVMVAWTGLEH+RIGRFDPPPPADE
Sbjct: 359 GGVASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADE 418

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQ 349
           PEDT+YDVRPRWPLGEEY EGRS ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 419 PEDTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466

[Close]
BLAST of CSPI03G09680 vs. NCBI nr
Match: XP_022924665.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 657.9 bits (1696), Expect = 4.9e-185
Identity = 332/348 (95.40%), Postives = 339/348 (97.41%), Query Frame = 0 

Query: 1   MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLP 60
           MAEEAHAQ IDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGS SLP
Sbjct: 119 MAEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLP 178

Query: 61  IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 120
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 179 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 238

Query: 121 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 180
           YDKTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRLAIE
Sbjct: 239 YDKTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIE 298

Query: 181 SRNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVS 240
           SRNID KIPLSSAS QDRSVRADIAASFQRVAV HLE++CERAIEWALKIEPSI+HLVVS
Sbjct: 299 SRNIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVS 358

Query: 241 GGVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADE 300
           GGVASNKYVR+QL  VVRKKGLQLVCPPPSLCTDNGVMVAWTGLEH+RIGRFDPPPPADE
Sbjct: 359 GGVASNKYVRSQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADE 418

Query: 301 PEDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQ 349
           PEDT+YDVRPRWPLGEEY EGRS ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 419 PEDTLYDVRPRWPLGEEYVEGRSVARSLRTARIHPSLTSIIQASLQQQ 466

[Close]
BLAST of CSPI03G09680 vs. TAIR 10
Match: AT2G45270.1 (glycoprotease 1 )

HSP 1 Score: 574.7 bits (1480), Expect = 5.0e-164
Identity = 280/348 (80.46%), Postives = 320/348 (91.95%), Query Frame = 0 

Query: 2   AEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPI 61
           AEEAH++VIDKVVQ+ ++KANLTE+DLSAVAVTIGPGLSLCLRVGV+KAR++AG+ SLPI
Sbjct: 130 AEEAHSRVIDKVVQDALDKANLTEKDLSAVAVTIGPGLSLCLRVGVRKARRVAGNFSLPI 189

Query: 62  VGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAY 121
           VGVHHMEAHALVARLVE+EL FPFMALLISGGHNLLVLA  LGQY QLGTT+DDAIGEA+
Sbjct: 190 VGVHHMEAHALVARLVEQELSFPFMALLISGGHNLLVLAHKLGQYTQLGTTVDDAIGEAF 249

Query: 122 DKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIES 181
           DKTAKWLGLD+ +SGGPA+EELA EGD KS+KF+ PMK HKDCNFSYAGLKTQVRLAIE+
Sbjct: 250 DKTAKWLGLDMHRSGGPAVEELALEGDAKSVKFNVPMKYHKDCNFSYAGLKTQVRLAIEA 309

Query: 182 RNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSG 241
           + ID K P+SSA+ +DR  RADIAASFQRVAVLHLE+KCERAI+WAL++EPSI+H+V+SG
Sbjct: 310 KEIDAKCPVSSATNEDRRNRADIAASFQRVAVLHLEEKCERAIDWALELEPSIKHMVISG 369

Query: 242 GVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEP 301
           GVASNKYVR +L  +V  K L+LVCPPPSLCTDNGVMVAWTGLEHFR+GR+DPPPPA EP
Sbjct: 370 GVASNKYVRLRLNNIVENKNLKLVCPPPSLCTDNGVMVAWTGLEHFRVGRYDPPPPATEP 429

Query: 302 EDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQA-SLQQQ 349
           ED +YD+RPRWPLGEEY +GRS ARS+RTARIHPSLTSII+A SLQQQ
Sbjct: 430 EDYVYDLRPRWPLGEEYAKGRSEARSMRTARIHPSLTSIIRADSLQQQ 477

[Close]
BLAST of CSPI03G09680 vs. TAIR 10
Match: AT4G22720.1 (Actin-like ATPase superfamily protein )

HSP 1 Score: 92.4 bits (228), Expect = 7.6e-19
Identity = 76/282 (26.95%), Postives = 128/282 (45.39%), Query Frame = 0 

Query: 6   HAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVH 65
           H   +  +V+  +E + +T  ++  +  T GPG+   L+V     R ++     PIV V+
Sbjct: 53  HLDHVLPLVKSALETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVN 112

Query: 66  HMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTA 125
           H  AH  + R+V      P + L +SGG N  V+A   G+Y   G TID A+G   D+ A
Sbjct: 113 HCVAHIEMGRVV-TGADDP-VVLYVSGG-NTQVIAYSEGRYRIFGETIDIAVGNCLDRFA 172

Query: 126 KWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNID 185
           + L L    S G  IE+LA++G+     F       K  + S++G+ + +    E +   
Sbjct: 173 RVLKLSNDPSPGYNIEQLAKKGE----NFIDLPYAVKGMDVSFSGILSYIETTAEEK--- 232

Query: 186 TKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVAS 245
                      +    AD+  S Q      L +  ERA+    K     + +++ GGV  
Sbjct: 233 --------LKNNECTPADLCYSLQETVFAMLVEITERAMAHCDK-----KDVLIVGGVGC 292

Query: 246 NKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHF 288
           N+ ++  +  +  ++  +L       C DNG M+A+TGL  F
Sbjct: 293 NERLQEMMRTMCSERDGKLFATDDRYCIDNGAMIAYTGLLAF 311

[Close]
BLAST of CSPI03G09680 vs. TAIR 10
Match: AT4G22720.2 (Actin-like ATPase superfamily protein )

HSP 1 Score: 92.4 bits (228), Expect = 7.6e-19
Identity = 76/282 (26.95%), Postives = 128/282 (45.39%), Query Frame = 0 

Query: 6   HAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIVGVH 65
           H   +  +V+  +E + +T  ++  +  T GPG+   L+V     R ++     PIV V+
Sbjct: 53  HLDHVLPLVKSALETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVN 112

Query: 66  HMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTA 125
           H  AH  + R+V      P + L +SGG N  V+A   G+Y   G TID A+G   D+ A
Sbjct: 113 HCVAHIEMGRVV-TGADDP-VVLYVSGG-NTQVIAYSEGRYRIFGETIDIAVGNCLDRFA 172

Query: 126 KWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIESRNID 185
           + L L    S G  IE+LA++G+     F       K  + S++G+ + +    E +   
Sbjct: 173 RVLKLSNDPSPGYNIEQLAKKGE----NFIDLPYAVKGMDVSFSGILSYIETTAEEK--- 232

Query: 186 TKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSGGVAS 245
                      +    AD+  S Q      L +  ERA+    K     + +++ GGV  
Sbjct: 233 --------LKNNECTPADLCYSLQETVFAMLVEITERAMAHCDK-----KDVLIVGGVGC 292

Query: 246 NKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHF 288
           N+ ++  +  +  ++  +L       C DNG M+A+TGL  F
Sbjct: 293 NERLQEMMRTMCSERDGKLFATDDRYCIDNGAMIAYTGLLAF 311

The following BLAST results are available for this feature:
BLAST of CSPI03G09680 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O221457.1e-16380.46Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Arabid... [more]
B3PQA43.3e-5942.27tRNA N6-adenosine threonylcarbamoyltransferase OS=Rhizobium etli (strain CIAT 65... [more]
Q2VYV27.3e-5941.05tRNA N6-adenosine threonylcarbamoyltransferase OS=Magnetospirillum magneticum (s... [more]
Q0P4K01.2e-5841.95Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Xenopu... [more]
Q1MAQ81.6e-5840.69tRNA N6-adenosine threonylcarbamoyltransferase OS=Rhizobium leguminosarum bv. vi... [more]
BLAST of CSPI03G09680 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L9384.7e-194100.00Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumi... [more]
A0A1S3AWD36.8e-19399.43Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumi... [more]
A0A6J1EFN52.3e-18595.40Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucurb... [more]
A0A6J1IT728.9e-18594.84Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucurb... [more]
A0A6J1D0B89.9e-18493.41Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Momord... [more]
BLAST of CSPI03G09680 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011650793.29.8e-194100.00probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucumis ... [more]
XP_008438179.11.4e-19299.43PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondria... [more]
XP_038877779.13.2e-18996.85probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Benincas... [more]
XP_023527336.12.8e-18595.69probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbit... [more]
XP_022924665.14.9e-18595.40probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbit... [more]
BLAST of CSPI03G09680 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G45270.15.0e-16480.46glycoprotease 1 [more]
AT4G22720.17.6e-1926.95Actin-like ATPase superfamily protein [more]
AT4G22720.27.6e-1926.95Actin-like ATPase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017861Kae1/TsaD familyPRINTSPR00789OSIALOPTASEcoord: 109..130
score: 59.49
coord: 31..51
score: 50.31
coord: 52..71
score: 37.61
coord: 237..246
score: 60.0
coord: 86..98
score: 52.17
IPR017861Kae1/TsaD familyTIGRFAMTIGR00329TIGR00329coord: 2..280
e-value: 3.6E-75
score: 251.0
NoneNo IPR availableGENE3D3.30.420.40coord: 2..288
e-value: 4.2E-99
score: 333.5
NoneNo IPR availableGENE3D3.30.420.40coord: 84..267
e-value: 4.2E-99
score: 333.5
IPR000905Gcp-like domainPFAMPF00814TsaDcoord: 2..281
e-value: 2.8E-79
score: 266.6
IPR000905Gcp-like domainPANTHERPTHR11735TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASEcoord: 1..326
IPR022450tRNA N6-adenosine threonylcarbamoyltransferase, TsaDPANTHERPTHR11735:SF6TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL-RELATEDcoord: 1..326
IPR022450tRNA N6-adenosine threonylcarbamoyltransferase, TsaDHAMAPMF_01445TsaDcoord: 2..314
score: 49.784424
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 2..77
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 58..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G09680.1CSPI03G09680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0002949 tRNA threonylcarbamoyladenosine modification
cellular_component GO:0005743 mitochondrial inner membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061711 N(6)-L-threonylcarbamoyladenine synthase activity