CSPI03G09010 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G09010
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptiondigalactosyldiacylglycerol synthase 2, chloroplastic
LocationChr3: 7418287 .. 7420234 (-)
RNA-Seq ExpressionCSPI03G09010
SyntenyCSPI03G09010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAGGAAGCAGCATATTGCAATTTTTACCACAGCATGTCTTCCATGGATGACTGGAACTGCAGTAAACCCTCTATTTCGTGCTGCTTATCTTTCTATGGATGGGGATAGAAAGGTCACACTGGTGGTTCCTTGGTTATCTTTAAGGGATCAAGAACTTGTATATCCTAGCAACACAACCTTCATCTTACCCTCACAACAAGAGAAATACATTCGGCAATGGCTAGAAGAAAGGACAGGATCAAAATTCAATTTCACCCTCCTATTTTATCCTGGGAAGGTAAACTGTAGCATGTCTTAATTACAATCCTTGTCTTAGACATGATGCTTCTTTACATGTGTTTGTTTGTGTAGCATGATTGAATTACAATCCTTGTCTTAGATATTGTACTCTTACTTCAGTTATGGCAACTGAAATTTCACTTAATTCTGTAGTTTGCTGTGGATAAAAGGAGCATTCTCCCTGTTGGTGATATTTCAGAAATTATTCCCGACCACGAATCAGATATTGCTGTCCTTGAGGAGCCTGAGCATTTAACATGGTATCATCATGGAAAGAGGTGGAAAAACAAATTTACATTGGTCGTAGGAATTATCCATACCAACTACTTGGAATATGTTAAGCGAGAGAAGAATGGGGCTGTTGAAGCTTTTCTTCTTGAATATATAAATAGATGGGTTGTTCATATCTATTGCCACAAGGTACTTGTGCTATGTGTTACTACAATTTGTTCATTGCAATCAATGTTTCAGTCTTCTTACTTCACTTCAGTGGAGTGTAATAGAATTTTGTCTTTTAATGATTTTTTTTCTATGGAGTGTAAATAGGTTGTTTTAATTATAAAGTGGATGGCTTATTCATCAATTCTTGTATAAGCTTTCAGGTGATTAGGTTATCTGCTGCAACCCAGGATTATCCAAAGTCCATTATCTGCAATGTTCATGGAGTCAATCCGAAATTCCTTGAGATAGGCAAGAAGAAGATGGAACAGCAACAAAATGGGGGGCAAGTTTTTGGCAAAGGTGCCTATTATATTGGTAAAATGATATGGAGCAAGGGGTACAGGGAATTGGTTAAACTTCTTAGCGATTACCAAAAGGACTTTGCTGAGCTTGAGGTTGATTTATTTGGAAATGGAGAGGATTCTGACGAAGTTCAGAAAGCTACTCAAGACTTGGAAGTATCTGTTAAAGTTCATCCTGGTCGTGATCACACTGATCCTATCTTCCACGAGTACGAACCTCTTTTAGCTTATGAATTTACTACTGTAGTAAAGCAAATAAAACAAAATGAATAAAAGATCAAGGGAGGCATTCACTACTGCAGCATCTTATGAATGAATTTTGCCGTAATGCATGACTGTTTAGAATCATTTTCCTCCGTTTGTTTAAATACATGTCCACTGGCTGATTTTAAGCTTTTTTTGTGTTCTTTTAGTTATAAAGTCTTCCTGAATCCAAGCACCACAGACGTTGTTTGCACTACCACAGCTGAGGCATTAGCAATGGGCAAATTCGTTGTATGCGCTAATCATCCCTCCAATGAGTTCTTCAAGCAATTTCCAAACTGCCTTCTCTATGACAACAACGATGGCTTTGTTAAGGCAGTTTTCAAAGCACTGCTTGAAGAGCCGGAACAGCTTACAGATGCGCAAAGACACGAGCTCTCGTGGGAAGCAGCAACAGAACGTTTCCTAAAGGCTGCTGAACTTGACCAGTCTCTTGCTAGAAAACCCCAGAAAACTCGTTCAAAGAAGTTTCTATCTCTATCATTGCAACTTGGTAGAAAATTTGATGATGCGACAGCATATATACATCACATGTCTCTTGGATTTGAGGGTTCTCGAAGAGTTTTCGGTGCCGTCCCTGGGAGCCTGCATCCAGATGAACAACAATGCAAAGAGCTCGGATTAGCTAGTCCTTCTAGCAAAAGAGGTTCGAAAAAG

mRNA sequence

ATGGATAGGAAGCAGCATATTGCAATTTTTACCACAGCATGTCTTCCATGGATGACTGGAACTGCAGTAAACCCTCTATTTCGTGCTGCTTATCTTTCTATGGATGGGGATAGAAAGGTCACACTGGTGGTTCCTTGGTTATCTTTAAGGGATCAAGAACTTGTATATCCTAGCAACACAACCTTCATCTTACCCTCACAACAAGAGAAATACATTCGGCAATGGCTAGAAGAAAGGACAGGATCAAAATTCAATTTCACCCTCCTATTTTATCCTGGGAAGTTTGCTGTGGATAAAAGGAGCATTCTCCCTGTTGGTGATATTTCAGAAATTATTCCCGACCACGAATCAGATATTGCTGTCCTTGAGGAGCCTGAGCATTTAACATGGTATCATCATGGAAAGAGGTGGAAAAACAAATTTACATTGGTCGTAGGAATTATCCATACCAACTACTTGGAATATGTTAAGCGAGAGAAGAATGGGGCTGTTGAAGCTTTTCTTCTTGAATATATAAATAGATGGGTTGTTCATATCTATTGCCACAAGGTGATTAGGTTATCTGCTGCAACCCAGGATTATCCAAAGTCCATTATCTGCAATGTTCATGGAGTCAATCCGAAATTCCTTGAGATAGGCAAGAAGAAGATGGAACAGCAACAAAATGGGGGGCAAGTTTTTGGCAAAGGTGCCTATTATATTGGTAAAATGATATGGAGCAAGGGGTACAGGGAATTGGTTAAACTTCTTAGCGATTACCAAAAGGACTTTGCTGAGCTTGAGGTTGATTTATTTGGAAATGGAGAGGATTCTGACGAAGTTCAGAAAGCTACTCAAGACTTGGAAGTATCTGTTAAAGTTCATCCTGGTCGTGATCACACTGATCCTATCTTCCACGATTATAAAGTCTTCCTGAATCCAAGCACCACAGACGTTGTTTGCACTACCACAGCTGAGGCATTAGCAATGGGCAAATTCGTTGTATGCGCTAATCATCCCTCCAATGAGTTCTTCAAGCAATTTCCAAACTGCCTTCTCTATGACAACAACGATGGCTTTGTTAAGGCAGTTTTCAAAGCACTGCTTGAAGAGCCGGAACAGCTTACAGATGCGCAAAGACACGAGCTCTCGTGGGAAGCAGCAACAGAACGTTTCCTAAAGGCTGCTGAACTTGACCAGTCTCTTGCTAGAAAACCCCAGAAAACTCGTTCAAAGAAGTTTCTATCTCTATCATTGCAACTTGGTAGAAAATTTGATGATGCGACAGCATATATACATCACATGTCTCTTGGATTTGAGGGTTCTCGAAGAGTTTTCGGTGCCGTCCCTGGGAGCCTGCATCCAGATGAACAACAATGCAAAGAGCTCGGATTAGCTAGTCCTTCTAGCAAAAGAGGTTCGAAAAAG

Coding sequence (CDS)

ATGGATAGGAAGCAGCATATTGCAATTTTTACCACAGCATGTCTTCCATGGATGACTGGAACTGCAGTAAACCCTCTATTTCGTGCTGCTTATCTTTCTATGGATGGGGATAGAAAGGTCACACTGGTGGTTCCTTGGTTATCTTTAAGGGATCAAGAACTTGTATATCCTAGCAACACAACCTTCATCTTACCCTCACAACAAGAGAAATACATTCGGCAATGGCTAGAAGAAAGGACAGGATCAAAATTCAATTTCACCCTCCTATTTTATCCTGGGAAGTTTGCTGTGGATAAAAGGAGCATTCTCCCTGTTGGTGATATTTCAGAAATTATTCCCGACCACGAATCAGATATTGCTGTCCTTGAGGAGCCTGAGCATTTAACATGGTATCATCATGGAAAGAGGTGGAAAAACAAATTTACATTGGTCGTAGGAATTATCCATACCAACTACTTGGAATATGTTAAGCGAGAGAAGAATGGGGCTGTTGAAGCTTTTCTTCTTGAATATATAAATAGATGGGTTGTTCATATCTATTGCCACAAGGTGATTAGGTTATCTGCTGCAACCCAGGATTATCCAAAGTCCATTATCTGCAATGTTCATGGAGTCAATCCGAAATTCCTTGAGATAGGCAAGAAGAAGATGGAACAGCAACAAAATGGGGGGCAAGTTTTTGGCAAAGGTGCCTATTATATTGGTAAAATGATATGGAGCAAGGGGTACAGGGAATTGGTTAAACTTCTTAGCGATTACCAAAAGGACTTTGCTGAGCTTGAGGTTGATTTATTTGGAAATGGAGAGGATTCTGACGAAGTTCAGAAAGCTACTCAAGACTTGGAAGTATCTGTTAAAGTTCATCCTGGTCGTGATCACACTGATCCTATCTTCCACGATTATAAAGTCTTCCTGAATCCAAGCACCACAGACGTTGTTTGCACTACCACAGCTGAGGCATTAGCAATGGGCAAATTCGTTGTATGCGCTAATCATCCCTCCAATGAGTTCTTCAAGCAATTTCCAAACTGCCTTCTCTATGACAACAACGATGGCTTTGTTAAGGCAGTTTTCAAAGCACTGCTTGAAGAGCCGGAACAGCTTACAGATGCGCAAAGACACGAGCTCTCGTGGGAAGCAGCAACAGAACGTTTCCTAAAGGCTGCTGAACTTGACCAGTCTCTTGCTAGAAAACCCCAGAAAACTCGTTCAAAGAAGTTTCTATCTCTATCATTGCAACTTGGTAGAAAATTTGATGATGCGACAGCATATATACATCACATGTCTCTTGGATTTGAGGGTTCTCGAAGAGTTTTCGGTGCCGTCCCTGGGAGCCTGCATCCAGATGAACAACAATGCAAAGAGCTCGGATTAGCTAGTCCTTCTAGCAAAAGAGGTTCGAAAAAG

Protein sequence

MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNTTFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIAVLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIYCHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWSKGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHDYKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKALLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDDATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK
Homology
BLAST of CSPI03G09010 vs. ExPASy Swiss-Prot
Match: Q6DW75 (Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Glycine max OX=3847 GN=DGD2 PE=2 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 3.4e-205
Identity = 341/464 (73.49%), Postives = 393/464 (84.70%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MD+K+HIAIFTTA LPW+TGTAVNPLFRAAYL+  G+R VTLV+PWLSL+DQ LVYP+N 
Sbjct: 1   MDKKEHIAIFTTASLPWLTGTAVNPLFRAAYLAKSGERDVTLVIPWLSLKDQRLVYPNNI 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TF  PS+ EKYI QWLEER G    F++ FYPGKF+ DKRSIL VGDISEIIPD  +DIA
Sbjct: 61  TFASPSEHEKYICQWLEERVGFTSGFSIQFYPGKFSRDKRSILAVGDISEIIPDKVADIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           VLEEPEHLTWYHHGKRWK KF LV+GIIHTNYLEYVKREKNG ++AFLL+Y+N WVV IY
Sbjct: 121 VLEEPEHLTWYHHGKRWKTKFRLVIGIIHTNYLEYVKREKNGVMQAFLLKYLNNWVVSIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDY  SIICNVHGVNPKFLEIGKKK EQQQ G   F KGAY+IGKMIWS
Sbjct: 181 CHKVIRLSAATQDYTGSIICNVHGVNPKFLEIGKKKREQQQKGEHAFTKGAYFIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGY+EL++LL D++K+ + LEVDLFG+GEDSDEVQKA + LE++V+VHP RDH D +FHD
Sbjct: 241 KGYKELLQLLKDHEKELSALEVDLFGSGEDSDEVQKAAEKLELAVRVHPARDHADALFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YK+FLNPSTTDVVCTTTAEALAMGK VVCANHPSN+FFKQFPNC  YD++DGFVK   KA
Sbjct: 301 YKLFLNPSTTDVVCTTTAEALAMGKIVVCANHPSNDFFKQFPNCWTYDDDDGFVKLTLKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L E+P Q TDAQRH+LSWEAAT+RFLKAA+LD+ L RK  +T S  FL+ SL L  K D+
Sbjct: 361 LAEQPAQPTDAQRHDLSWEAATKRFLKAADLDKPLERKLSRTTS-NFLAASLNLQEKVDE 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSK 465
           A+AY+HH++ GFE SRR+FGA+P SL PDE+  KELGL   S+K
Sbjct: 421 ASAYVHHVASGFEVSRRIFGAIPDSLQPDEELRKELGLTDASTK 463

BLAST of CSPI03G09010 vs. ExPASy Swiss-Prot
Match: Q6DW73 (Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Lotus japonicus OX=34305 GN=DGD2 PE=2 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 2.2e-204
Identity = 339/464 (73.06%), Postives = 393/464 (84.70%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           M +KQHIAIFTTA LPW+TGTAVNPLFRAAYLS DG+R VTLV+PWLSL+DQ LVYP+N 
Sbjct: 1   MGKKQHIAIFTTASLPWLTGTAVNPLFRAAYLSKDGERDVTLVIPWLSLKDQALVYPNNI 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TF  PS+ EKYIRQWLEER G    F++ FYPGKF+ DKRSIL VGDISE+IPD E+DIA
Sbjct: 61  TFASPSEHEKYIRQWLEERVGFTSGFSIKFYPGKFSRDKRSILAVGDISEVIPDKEADIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           VLEEPEHLTW+HHGKRWK KF LV+GIIHTNYLEYVKREKNG ++AFLL+Y+N WVV IY
Sbjct: 121 VLEEPEHLTWFHHGKRWKTKFRLVIGIIHTNYLEYVKREKNGQMQAFLLKYLNNWVVGIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDY  SI+CNVHGVNPKFLEIGKKK EQQQNG Q F KGAY+IGKM+WS
Sbjct: 181 CHKVIRLSAATQDYSGSIVCNVHGVNPKFLEIGKKKREQQQNGDQAFTKGAYFIGKMVWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGY+EL+ L  ++QK+ + LEVDLFG+GEDSDEVQKA + LE++V+VHP RDH D +FHD
Sbjct: 241 KGYKELLHLFKNHQKELSALEVDLFGSGEDSDEVQKAAKKLEMAVRVHPARDHADALFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YK+FLNPSTTDVVCTTTAEALAMGK VVCANH SNEFFKQFPNC  +D + GFV+ + KA
Sbjct: 301 YKLFLNPSTTDVVCTTTAEALAMGKIVVCANHCSNEFFKQFPNCWTFDESKGFVQLILKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L EEP QLTDAQRH+LSWEAATERFLKAAELD+   +K  ++ S  ++S SL L +  DD
Sbjct: 361 LAEEPAQLTDAQRHDLSWEAATERFLKAAELDKPFEKKLSRSTS-IYMSTSLNLQQTVDD 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSK 465
           A+AY+HH++ GFE SRR+FGA+PGSL PDE+  KELGL+    K
Sbjct: 421 ASAYVHHVASGFEISRRMFGAIPGSLKPDEELSKELGLSDSGRK 463

BLAST of CSPI03G09010 vs. ExPASy Swiss-Prot
Match: Q8W1S1 (Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGD2 PE=1 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 4.4e-192
Identity = 309/467 (66.17%), Postives = 389/467 (83.30%), Query Frame = 0

Query: 3   RKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNTTF 62
           ++QHIAIFTTA +PW+TGTAVNPLFRAAYL+ DG+R+VTLV+PWL+L+ Q+LVYP++ TF
Sbjct: 5   QEQHIAIFTTASIPWLTGTAVNPLFRAAYLANDGERRVTLVIPWLTLKHQKLVYPNSITF 64

Query: 63  ILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIAVL 122
             PS+QE Y+RQWLEER   +  F + FYPGKFA+DKRSILPVGDIS+ IPD E+DIAVL
Sbjct: 65  SSPSEQEAYVRQWLEERVSFRLAFEIRFYPGKFAIDKRSILPVGDISDAIPDEEADIAVL 124

Query: 123 EEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIYCH 182
           EEPEHLTW+HHG++WK KF  V+GI+HTNYLEYVKREK G V+AF L+Y+N WVV IYCH
Sbjct: 125 EEPEHLTWFHHGQKWKTKFNYVIGIVHTNYLEYVKREKQGRVKAFFLKYLNSWVVGIYCH 184

Query: 183 KVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWSKG 242
           KVIRLSAATQ+YPKSI+CNVHGVNPKFLEIG +K+EQQ+   Q F KGAYYIGKM+WSKG
Sbjct: 185 KVIRLSAATQEYPKSIVCNVHGVNPKFLEIGLRKLEQQKLQEQPFTKGAYYIGKMVWSKG 244

Query: 243 YRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHDYK 302
           Y+EL+KLL  +QK+ AELEVDL+G+GEDS+E+++A + L+++V V+PGRDH D +FH+YK
Sbjct: 245 YKELLKLLEKHQKELAELEVDLYGDGEDSEEIKEAARKLDLTVNVYPGRDHADSLFHNYK 304

Query: 303 VFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKALL 362
           VFLNPSTTDVVCTTTAEALAMGK VVCANH SN+FFKQFPNC  YD+  GFV+A  KAL 
Sbjct: 305 VFLNPSTTDVVCTTTAEALAMGKIVVCANHISNKFFKQFPNCRTYDDGQGFVRATLKALG 364

Query: 363 EEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDDAT 422
           E+P QLT+ QRHELSWEAAT+RF+K ++L++        ++   F S S+ +G+  +D +
Sbjct: 365 EQPSQLTEQQRHELSWEAATQRFIKVSDLNRLSRADSNLSKRSVFASSSISVGKNLEDMS 424

Query: 423 AYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLA----SPSSKR 466
           AYIH ++ GFE SR  FGA+PGSL PDE+ C++LGL+    SP++++
Sbjct: 425 AYIHFLASGFEASRTAFGAIPGSLQPDEELCRDLGLSLNTPSPNTRK 471

BLAST of CSPI03G09010 vs. ExPASy Swiss-Prot
Match: Q6DW76 (Digalactosyldiacylglycerol synthase 1, chloroplastic OS=Glycine max OX=3847 GN=DGD1 PE=2 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 3.4e-160
Identity = 269/468 (57.48%), Postives = 346/468 (73.93%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           +D ++H+AI TTA LPWMTGTAVNPLFRAAYLS    +KVTL+VPWL   DQELVYPSN 
Sbjct: 307 LDNERHVAIVTTASLPWMTGTAVNPLFRAAYLSQSAKQKVTLLVPWLCKSDQELVYPSNL 366

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TF  P +QE YIR WLEER G K +F + FYPGKF+  +RSI+P GD S+ IP  ++DIA
Sbjct: 367 TFTSPEEQEAYIRSWLEERIGFKADFKISFYPGKFSEARRSIIPAGDTSQFIPSRDADIA 426

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           +LEEPEHL WYHHGKRW +KF  VVGI+HTNYLEY+KREKNGA++AFL+++IN WV   Y
Sbjct: 427 ILEEPEHLNWYHHGKRWTDKFNHVVGIVHTNYLEYIKREKNGALQAFLVKHINNWVTRAY 486

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKV+RLSAATQD PKS+ICNVHGVNPKFL+IG+K   +++ G + F KGAY++GKM+W+
Sbjct: 487 CHKVLRLSAATQDLPKSVICNVHGVNPKFLKIGEKIAAERELGQKAFTKGAYFLGKMVWA 546

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGY+EL+ LL+ ++ D    ++D+FGNGED++EVQ A + L++++    GRDH D   H 
Sbjct: 547 KGYKELIDLLAKHKADLDGFKLDVFGNGEDANEVQSAARRLDLNLNFQKGRDHADDSLHR 606

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVF+NPS +DV+CT TAEALAMGKFVVCA+HPSNEFF+ FPNCL Y  ++ FV  V +A
Sbjct: 607 YKVFINPSISDVLCTATAEALAMGKFVVCADHPSNEFFRSFPNCLTYRTSEDFVTKVKEA 666

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSK----KFLSLSL---Q 420
           L  EP  LT  QR++LSWEAAT+RF++ +ELD  L ++    +S+    K ++ S     
Sbjct: 667 LENEPYPLTPEQRYQLSWEAATQRFMEYSELDGILNKENNGEKSRVDKGKLIAKSASMPN 726

Query: 421 LGRKFDDATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASP 462
           L    D   A+ H+   G E  R   GA+PG+   D+Q CK+L L  P
Sbjct: 727 LTELVDGGLAFAHYCLTGNEFLRLCTGAIPGTRDYDKQHCKDLHLLPP 774

BLAST of CSPI03G09010 vs. ExPASy Swiss-Prot
Match: Q6DW74 (Digalactosyldiacylglycerol synthase 1, chloroplastic OS=Lotus japonicus OX=34305 GN=DGD1 PE=2 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 5.0e-156
Identity = 264/468 (56.41%), Postives = 341/468 (72.86%), Query Frame = 0

Query: 2   DRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNTT 61
           D ++H+AI TTA LPWMTGTAVNPLFRAAYLS    +KVTL+VPWL   DQELVYPSN T
Sbjct: 311 DNERHVAIVTTASLPWMTGTAVNPLFRAAYLSQSEKQKVTLLVPWLCKSDQELVYPSNLT 370

Query: 62  FILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIAV 121
           F  P +QE YIR WLEER G K +F + FYPGKF+  +RSI+P GD ++ IP  ++DIA+
Sbjct: 371 FTSPEEQEGYIRNWLEERIGFKADFKISFYPGKFSQARRSIIPAGDTAQFIPSKDADIAI 430

Query: 122 LEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIYC 181
           LEEPEHL WYHHG RW +KF  VVGI+HTNYLEY+KREKNGA++AFL+++IN WV   YC
Sbjct: 431 LEEPEHLNWYHHGTRWTDKFNHVVGIVHTNYLEYIKREKNGALQAFLVKHINNWVARAYC 490

Query: 182 HKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWSK 241
            KV+RLSAATQD PKS++CNVHGVNPKFL+IG+    +++ G + F KGAY++GKM+W+K
Sbjct: 491 DKVLRLSAATQDLPKSVVCNVHGVNPKFLKIGESIAAERELGQKGFTKGAYFLGKMVWAK 550

Query: 242 GYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHDY 301
           GY+EL+ LL+ ++ D   +++D+FGNGED++EVQ A +  ++++    GRDH D   H Y
Sbjct: 551 GYKELIDLLAKHKADLDGVKLDVFGNGEDANEVQSAARRFDLNLNFQKGRDHADDSLHRY 610

Query: 302 KVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKAL 361
           KVF+NPS +DV+CT TAEALAMGKFVVCA+HPSNEFF+ FPNCL Y   + F   V +AL
Sbjct: 611 KVFINPSISDVLCTATAEALAMGKFVVCADHPSNEFFRSFPNCLTYKTPEDFAVKVKEAL 670

Query: 362 LEEPEQLTDAQRHELSWEAATERFLKAAELDQSLAR-----KPQKTRSKKFLSLSL---Q 421
             EP  LT  QR++LSWEAAT+RF++ +ELD+ L +     KP K  ++K ++ S     
Sbjct: 671 ANEPYPLTPEQRYQLSWEAATQRFMEYSELDKVLNKEKDGAKPSK-NNRKIMAKSASMPN 730

Query: 422 LGRKFDDATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASP 462
           L    D   A+ H+   G E  R   GA PG+   D+Q CK+L L  P
Sbjct: 731 LTELVDGGLAFAHYCLTGNEFLRLCTGATPGTRDYDKQHCKDLNLLPP 777

BLAST of CSPI03G09010 vs. ExPASy TrEMBL
Match: A0A0A0L6B2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122630 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 4.6e-277
Identity = 469/469 (100.00%), Postives = 469/469 (100.00%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT
Sbjct: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY
Sbjct: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD
Sbjct: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD
Sbjct: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK
Sbjct: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 469

BLAST of CSPI03G09010 vs. ExPASy TrEMBL
Match: A0A1S3AV60 (digalactosyldiacylglycerol synthase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483294 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 1.8e-265
Identity = 445/469 (94.88%), Postives = 462/469 (98.51%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MDRKQHIAIFTTA LPWMTGTAVNPLFRAAYLSMDGDRK+TLVVPWLSLRDQELVYPSNT
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSMDGDRKITLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TFILPSQQEKYIRQWLEERTGSKFNFTL FYPGKFAVDKRSILPVGDISEII + ESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLQFYPGKFAVDKRSILPVGDISEIIHEDESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHGKRWKNKFTLV+GIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGKRWKNKFTLVIGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKK+EQQ+NGGQVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKIEQQKNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYREL+KLL+DYQKD AELEVDLFGNGED+DEVQKA+QDLE+SVKVHPGRDHTDPIFHD
Sbjct: 241 KGYRELLKLLTDYQKDLAELEVDLFGNGEDADEVQKASQDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNC+LYDN+DGFVKA+FKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCILYDNSDGFVKAIFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L EEPEQLTDAQRHELSWEAATERFLKAAELDQ LARKPQKTRSKKFLSLSLQLGRKFDD
Sbjct: 361 LSEEPEQLTDAQRHELSWEAATERFLKAAELDQPLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           A+AYIHHM+LGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSS+RGSKK
Sbjct: 421 ASAYIHHMTLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSRRGSKK 469

BLAST of CSPI03G09010 vs. ExPASy TrEMBL
Match: A0A5D3D3K6 (Digalactosyldiacylglycerol synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004490 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 1.8e-265
Identity = 445/469 (94.88%), Postives = 462/469 (98.51%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MDRKQHIAIFTTA LPWMTGTAVNPLFRAAYLSMDGDRK+TLVVPWLSLRDQELVYPSNT
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSMDGDRKITLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TFILPSQQEKYIRQWLEERTGSKFNFTL FYPGKFAVDKRSILPVGDISEII + ESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLQFYPGKFAVDKRSILPVGDISEIIHEDESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHGKRWKNKFTLV+GIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGKRWKNKFTLVIGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKK+EQQ+NGGQVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKIEQQKNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYREL+KLL+DYQKD AELEVDLFGNGED+DEVQKA+QDLE+SVKVHPGRDHTDPIFHD
Sbjct: 241 KGYRELLKLLTDYQKDLAELEVDLFGNGEDADEVQKASQDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNC+LYDN+DGFVKA+FKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCILYDNSDGFVKAIFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L EEPEQLTDAQRHELSWEAATERFLKAAELDQ LARKPQKTRSKKFLSLSLQLGRKFDD
Sbjct: 361 LSEEPEQLTDAQRHELSWEAATERFLKAAELDQPLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           A+AYIHHM+LGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSS+RGSKK
Sbjct: 421 ASAYIHHMTLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSRRGSKK 469

BLAST of CSPI03G09010 vs. ExPASy TrEMBL
Match: A0A6J1D1F6 (digalactosyldiacylglycerol synthase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016394 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.0e-249
Identity = 419/469 (89.34%), Postives = 448/469 (95.52%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MDRKQHIAIFTTA LPWMTGTAVNPLFRAAYLS+ G R+VTLVVPWLSLRDQELVYPS+T
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TFILPSQQEKYIR+WLEERTGSKFNF+L FYPGKF+VDKRSILPVGDISEIIPD ESDIA
Sbjct: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHG+RWKNKF LV+GIIHTNYLEYVKREKNGA+EAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIG KKMEQ QNG QVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYREL+KLLSDYQKD A LE+DLFGNGED+D+VQKAT+DLE+SV+VH GRDHTDPIFHD
Sbjct: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC+LYDN+DGFV+AVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L EEPEQLTDAQRHEL+WEAATERFLKA+ELDQ L RKPQK RSKKFLSLSLQL RKF+D
Sbjct: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           A+AYIHH +LGFEG RR+FGAVPGSL+PDEQQCKELGLA P SKRGSKK
Sbjct: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLA-PPSKRGSKK 468

BLAST of CSPI03G09010 vs. ExPASy TrEMBL
Match: A0A6J1IFT4 (digalactosyldiacylglycerol synthase 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111474080 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 7.9e-245
Identity = 410/469 (87.42%), Postives = 438/469 (93.39%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MD KQHIAIFTTA LPWMTGTAVNPLFRAAYLS+  DRKVTLVVPWLS RDQELVYPSNT
Sbjct: 1   MDTKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGEDRKVTLVVPWLSKRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TF+LPSQQEKYIR+WLEER GSKFNF L FYPGKFAVDKRSILPVGDISEIIPD ESDIA
Sbjct: 61  TFVLPSQQEKYIREWLEERIGSKFNFNLQFYPGKFAVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHG+RWK KF  VVGIIHTNYLEYVKREKNGAVEAFLLE+INRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGRRWKKKFKWVVGIIHTNYLEYVKREKNGAVEAFLLEHINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKS+ICNVHGVNPKFLEIGKKKMEQ+Q+  QVFGKG YYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSVICNVHGVNPKFLEIGKKKMEQRQDEEQVFGKGVYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYREL+KLLSD+QKD A +EVDLFGNGEDSDEVQKA +DLE+SVKVHPGRDHTDPIFHD
Sbjct: 241 KGYRELLKLLSDHQKDLAGIEVDLFGNGEDSDEVQKAARDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVF+NPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC L D +D FV+ +FKA
Sbjct: 301 YKVFVNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCFLCDKSDAFVRGIFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L EEPE LT+ Q HELSWEAATERFL AAELDQ LA+KPQ+TRSKKFLS+SLQLGRKFDD
Sbjct: 361 LTEEPEPLTNVQMHELSWEAATERFLTAAELDQPLAKKPQRTRSKKFLSMSLQLGRKFDD 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           A+AYIHH++LGFEGSRR FGAVPGSLHPDEQQCKELG+ASPS KRGSKK
Sbjct: 421 ASAYIHHVTLGFEGSRRAFGAVPGSLHPDEQQCKELGIASPSGKRGSKK 469

BLAST of CSPI03G09010 vs. NCBI nr
Match: XP_004133875.1 (digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis sativus] >KGN56539.1 hypothetical protein Csa_010433 [Cucumis sativus])

HSP 1 Score: 963.0 bits (2488), Expect = 9.5e-277
Identity = 469/469 (100.00%), Postives = 469/469 (100.00%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT
Sbjct: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY
Sbjct: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD
Sbjct: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD
Sbjct: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK
Sbjct: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 469

BLAST of CSPI03G09010 vs. NCBI nr
Match: XP_008438079.1 (PREDICTED: digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis melo] >XP_008438080.1 PREDICTED: digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis melo] >KAA0048985.1 digalactosyldiacylglycerol synthase 2 [Cucumis melo var. makuwa] >TYK17579.1 digalactosyldiacylglycerol synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 924.5 bits (2388), Expect = 3.7e-265
Identity = 445/469 (94.88%), Postives = 462/469 (98.51%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MDRKQHIAIFTTA LPWMTGTAVNPLFRAAYLSMDGDRK+TLVVPWLSLRDQELVYPSNT
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSMDGDRKITLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TFILPSQQEKYIRQWLEERTGSKFNFTL FYPGKFAVDKRSILPVGDISEII + ESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLQFYPGKFAVDKRSILPVGDISEIIHEDESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHGKRWKNKFTLV+GIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGKRWKNKFTLVIGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKK+EQQ+NGGQVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKIEQQKNGGQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYREL+KLL+DYQKD AELEVDLFGNGED+DEVQKA+QDLE+SVKVHPGRDHTDPIFHD
Sbjct: 241 KGYRELLKLLTDYQKDLAELEVDLFGNGEDADEVQKASQDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNC+LYDN+DGFVKA+FKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCILYDNSDGFVKAIFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L EEPEQLTDAQRHELSWEAATERFLKAAELDQ LARKPQKTRSKKFLSLSLQLGRKFDD
Sbjct: 361 LSEEPEQLTDAQRHELSWEAATERFLKAAELDQPLARKPQKTRSKKFLSLSLQLGRKFDD 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           A+AYIHHM+LGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSS+RGSKK
Sbjct: 421 ASAYIHHMTLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSRRGSKK 469

BLAST of CSPI03G09010 vs. NCBI nr
Match: XP_038878183.1 (digalactosyldiacylglycerol synthase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 888.6 bits (2295), Expect = 2.3e-254
Identity = 429/469 (91.47%), Postives = 449/469 (95.74%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MDR+QHIAIFTTA LPWMTGTAVNPLFRAAYLS+ GDRK+TLVVPWLSLRDQELVYPSNT
Sbjct: 1   MDREQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGDRKITLVVPWLSLRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TFILPSQQEKYIRQWLEERTGSKFNFTL FYPGKFAVDKRSILPVGDISEIIPD ESDIA
Sbjct: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLQFYPGKFAVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHGKRWKNKF LV+GIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGKRWKNKFKLVIGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSI CNVHGVNPKFLEIGKKKMEQQQ GGQVFG+GAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSITCNVHGVNPKFLEIGKKKMEQQQIGGQVFGRGAYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYREL+KLLS+YQ +   +EVDLFGNGED+DEVQKATQDLE+SV+VHPGRDHTDPIFHD
Sbjct: 241 KGYRELLKLLSNYQTELDGIEVDLFGNGEDADEVQKATQDLELSVRVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC+LYDN DGFVKAVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCILYDNGDGFVKAVFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L EEPEQLTDAQRH LSWEAATERFL AAELDQ LARKPQ+TRSKKFLSLS QLGRKFDD
Sbjct: 361 LNEEPEQLTDAQRHVLSWEAATERFLTAAELDQPLARKPQRTRSKKFLSLSSQLGRKFDD 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           A+AYIHHM+LGFEG RRVFGAVPGSL PDEQQCKELGLA PSSKRGS+K
Sbjct: 421 ASAYIHHMTLGFEGPRRVFGAVPGSLDPDEQQCKELGLARPSSKRGSRK 469

BLAST of CSPI03G09010 vs. NCBI nr
Match: XP_022147482.1 (digalactosyldiacylglycerol synthase 2, chloroplastic [Momordica charantia] >XP_022147483.1 digalactosyldiacylglycerol synthase 2, chloroplastic [Momordica charantia])

HSP 1 Score: 870.2 bits (2247), Expect = 8.3e-249
Identity = 419/469 (89.34%), Postives = 448/469 (95.52%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MDRKQHIAIFTTA LPWMTGTAVNPLFRAAYLS+ G R+VTLVVPWLSLRDQELVYPS+T
Sbjct: 1   MDRKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGYRRVTLVVPWLSLRDQELVYPSST 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TFILPSQQEKYIR+WLEERTGSKFNF+L FYPGKF+VDKRSILPVGDISEIIPD ESDIA
Sbjct: 61  TFILPSQQEKYIRKWLEERTGSKFNFSLQFYPGKFSVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHG+RWKNKF LV+GIIHTNYLEYVKREKNGA+EAFLLEYINRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGRRWKNKFKLVIGIIHTNYLEYVKREKNGALEAFLLEYINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIG KKMEQ QNG QVFGKGAYYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGTKKMEQLQNGEQVFGKGAYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYREL+KLLSDYQKD A LE+DLFGNGED+D+VQKAT+DLE+SV+VH GRDHTDPIFHD
Sbjct: 241 KGYRELLKLLSDYQKDLAGLEIDLFGNGEDADQVQKATEDLELSVRVHAGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVFLNPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC+LYDN+DGFV+AVFKA
Sbjct: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCMLYDNSDGFVRAVFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L EEPEQLTDAQRHEL+WEAATERFLKA+ELDQ L RKPQK RSKKFLSLSLQL RKF+D
Sbjct: 361 LTEEPEQLTDAQRHELTWEAATERFLKASELDQPLMRKPQKNRSKKFLSLSLQLSRKFED 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           A+AYIHH +LGFEG RR+FGAVPGSL+PDEQQCKELGLA P SKRGSKK
Sbjct: 421 ASAYIHHATLGFEGPRRMFGAVPGSLYPDEQQCKELGLA-PPSKRGSKK 468

BLAST of CSPI03G09010 vs. NCBI nr
Match: XP_023540326.1 (digalactosyldiacylglycerol synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540327.1 digalactosyldiacylglycerol synthase 2, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo] >XP_023540328.1 digalactosyldiacylglycerol synthase 2, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 858.2 bits (2216), Expect = 3.3e-245
Identity = 410/469 (87.42%), Postives = 439/469 (93.60%), Query Frame = 0

Query: 1   MDRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNT 60
           MD KQHIAIFTTA LPWMTGTAVNPLFRAAYLS+ GDRKVTLVVPWLS+RDQELVYPSNT
Sbjct: 1   MDTKQHIAIFTTASLPWMTGTAVNPLFRAAYLSIGGDRKVTLVVPWLSIRDQELVYPSNT 60

Query: 61  TFILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIA 120
           TF+LPSQQEKYIR+WLEER GSKFNF L FYPGKFAVDKRSILPVGDISEIIPD ESDIA
Sbjct: 61  TFVLPSQQEKYIREWLEERIGSKFNFNLQFYPGKFAVDKRSILPVGDISEIIPDDESDIA 120

Query: 121 VLEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIY 180
           +LEEPEHLTWYHHG+RWK KF  VVGIIHTNYLEYVKREKNGAVEAFLLE+INRWVVHIY
Sbjct: 121 ILEEPEHLTWYHHGRRWKKKFKWVVGIIHTNYLEYVKREKNGAVEAFLLEHINRWVVHIY 180

Query: 181 CHKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWS 240
           CHKVIRLSAATQDYPKS+ICNVHGVNPKFLEIGKKKMEQ+Q+  QVFGKG YYIGKMIWS
Sbjct: 181 CHKVIRLSAATQDYPKSVICNVHGVNPKFLEIGKKKMEQRQDEDQVFGKGVYYIGKMIWS 240

Query: 241 KGYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHD 300
           KGYREL+KLL D+QKD A +EVDLFGNGEDSDEVQKA QDLE+SVKVHPGRDHTDPIFHD
Sbjct: 241 KGYRELLKLLGDHQKDLAGIEVDLFGNGEDSDEVQKAAQDLELSVKVHPGRDHTDPIFHD 300

Query: 301 YKVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKA 360
           YKVF+NPSTTDVVCTTTAEALAMGKFVVCA+HPSNEFF+QFPNC L D +D FV+ +FKA
Sbjct: 301 YKVFVNPSTTDVVCTTTAEALAMGKFVVCADHPSNEFFRQFPNCFLCDKSDAFVRGIFKA 360

Query: 361 LLEEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDD 420
           L EEPE LT+AQ HELSWEAATERFL AAELDQ LA+KPQ+TRSKKFLS+SLQLGRKFDD
Sbjct: 361 LTEEPEPLTNAQMHELSWEAATERFLTAAELDQPLAKKPQRTRSKKFLSMSLQLGRKFDD 420

Query: 421 ATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASPSSKRGSKK 470
           A+AYIHH++LGFEGSRR FGAVPGSLHPDEQQCKELG+ASPS+KRG  K
Sbjct: 421 ASAYIHHVTLGFEGSRRAFGAVPGSLHPDEQQCKELGIASPSNKRGWNK 469

BLAST of CSPI03G09010 vs. TAIR 10
Match: AT4G00550.1 (digalactosyl diacylglycerol deficient 2 )

HSP 1 Score: 672.2 bits (1733), Expect = 3.1e-193
Identity = 309/467 (66.17%), Postives = 389/467 (83.30%), Query Frame = 0

Query: 3   RKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNTTF 62
           ++QHIAIFTTA +PW+TGTAVNPLFRAAYL+ DG+R+VTLV+PWL+L+ Q+LVYP++ TF
Sbjct: 5   QEQHIAIFTTASIPWLTGTAVNPLFRAAYLANDGERRVTLVIPWLTLKHQKLVYPNSITF 64

Query: 63  ILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIAVL 122
             PS+QE Y+RQWLEER   +  F + FYPGKFA+DKRSILPVGDIS+ IPD E+DIAVL
Sbjct: 65  SSPSEQEAYVRQWLEERVSFRLAFEIRFYPGKFAIDKRSILPVGDISDAIPDEEADIAVL 124

Query: 123 EEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIYCH 182
           EEPEHLTW+HHG++WK KF  V+GI+HTNYLEYVKREK G V+AF L+Y+N WVV IYCH
Sbjct: 125 EEPEHLTWFHHGQKWKTKFNYVIGIVHTNYLEYVKREKQGRVKAFFLKYLNSWVVGIYCH 184

Query: 183 KVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWSKG 242
           KVIRLSAATQ+YPKSI+CNVHGVNPKFLEIG +K+EQQ+   Q F KGAYYIGKM+WSKG
Sbjct: 185 KVIRLSAATQEYPKSIVCNVHGVNPKFLEIGLRKLEQQKLQEQPFTKGAYYIGKMVWSKG 244

Query: 243 YRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHDYK 302
           Y+EL+KLL  +QK+ AELEVDL+G+GEDS+E+++A + L+++V V+PGRDH D +FH+YK
Sbjct: 245 YKELLKLLEKHQKELAELEVDLYGDGEDSEEIKEAARKLDLTVNVYPGRDHADSLFHNYK 304

Query: 303 VFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKALL 362
           VFLNPSTTDVVCTTTAEALAMGK VVCANH SN+FFKQFPNC  YD+  GFV+A  KAL 
Sbjct: 305 VFLNPSTTDVVCTTTAEALAMGKIVVCANHISNKFFKQFPNCRTYDDGQGFVRATLKALG 364

Query: 363 EEPEQLTDAQRHELSWEAATERFLKAAELDQSLARKPQKTRSKKFLSLSLQLGRKFDDAT 422
           E+P QLT+ QRHELSWEAAT+RF+K ++L++        ++   F S S+ +G+  +D +
Sbjct: 365 EQPSQLTEQQRHELSWEAATQRFIKVSDLNRLSRADSNLSKRSVFASSSISVGKNLEDMS 424

Query: 423 AYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLA----SPSSKR 466
           AYIH ++ GFE SR  FGA+PGSL PDE+ C++LGL+    SP++++
Sbjct: 425 AYIHFLASGFEASRTAFGAIPGSLQPDEELCRDLGLSLNTPSPNTRK 471

BLAST of CSPI03G09010 vs. TAIR 10
Match: AT3G11670.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 544.3 bits (1401), Expect = 9.8e-155
Identity = 256/465 (55.05%), Postives = 333/465 (71.61%), Query Frame = 0

Query: 2   DRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNTT 61
           + K+H+AI TTA LPWMTGTAVNPLFRAAYL+    + VTLVVPWL   DQELVYP+N T
Sbjct: 341 ENKRHVAIVTTASLPWMTGTAVNPLFRAAYLAKAAKQSVTLVVPWLCESDQELVYPNNLT 400

Query: 62  FILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIAV 121
           F  P +QE YIR+WLEER G K +F + FYPGKF+ ++RSI P GD S+ I   ++DIA+
Sbjct: 401 FSSPEEQESYIRKWLEERIGFKADFKISFYPGKFSKERRSIFPAGDTSQFISSKDADIAI 460

Query: 122 LEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIYC 181
           LEEPEHL WY+HGKRW +KF  VVGI+HTNYLEY+KREKNGA++AF + ++N WV   YC
Sbjct: 461 LEEPEHLNWYYHGKRWTDKFNHVVGIVHTNYLEYIKREKNGALQAFFVNHVNNWVTRAYC 520

Query: 182 HKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWSK 241
            KV+RLSAATQD PKS++CNVHGVNPKFL IG+K  E++  G Q F KGAY++GKM+W+K
Sbjct: 521 DKVLRLSAATQDLPKSVVCNVHGVNPKFLMIGEKIAEERSRGEQAFSKGAYFLGKMVWAK 580

Query: 242 GYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFHDY 301
           GYREL+ L++ ++ +     +D++GNGED+ EVQ+A +  ++++    GRDH D   H Y
Sbjct: 581 GYRELIDLMAKHKSELGSFNLDVYGNGEDAVEVQRAAKKHDLNLNFLKGRDHADDALHKY 640

Query: 302 KVFLNPSTTDVVCTTTAEALAMGKFVVCANHPSNEFFKQFPNCLLYDNNDGFVKAVFKAL 361
           KVF+NPS +DV+CT TAEALAMGKFVVCA+HPSNEFF+ FPNCL Y  ++ FV  V +A+
Sbjct: 641 KVFINPSISDVLCTATAEALAMGKFVVCADHPSNEFFRSFPNCLTYKTSEDFVSKVQEAM 700

Query: 362 LEEPEQLTDAQRHELSWEAATERFLKAAELDQSL-----ARKPQKTRSKKFLSLSLQLGR 421
            +EP  LT  Q + LSWEAAT+RF++ ++LD+ L      RK +K+RS            
Sbjct: 701 TKEPLPLTPEQMYNLSWEAATQRFMEYSDLDKILNNGEGGRKMRKSRSVP------SFNE 760

Query: 422 KFDDATAYIHHMSLGFEGSRRVFGAVPGSLHPDEQQCKELGLASP 462
             D   A+ H++  G +  R   GA P +   D Q CK+L L  P
Sbjct: 761 VVDGGLAFSHYVLTGNDFLRLCTGATPRTKDYDNQHCKDLNLVPP 799

BLAST of CSPI03G09010 vs. TAIR 10
Match: AT3G11670.2 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 401.7 bits (1031), Expect = 7.8e-112
Identity = 177/298 (59.40%), Postives = 229/298 (76.85%), Query Frame = 0

Query: 2   DRKQHIAIFTTACLPWMTGTAVNPLFRAAYLSMDGDRKVTLVVPWLSLRDQELVYPSNTT 61
           + K+H+AI TTA LPWMTGTAVNPLFRAAYL+    + VTLVVPWL   DQELVYP+N T
Sbjct: 341 ENKRHVAIVTTASLPWMTGTAVNPLFRAAYLAKAAKQSVTLVVPWLCESDQELVYPNNLT 400

Query: 62  FILPSQQEKYIRQWLEERTGSKFNFTLLFYPGKFAVDKRSILPVGDISEIIPDHESDIAV 121
           F  P +QE YIR+WLEER G K +F + FYPGKF+ ++RSI P GD S+ I   ++DIA+
Sbjct: 401 FSSPEEQESYIRKWLEERIGFKADFKISFYPGKFSKERRSIFPAGDTSQFISSKDADIAI 460

Query: 122 LEEPEHLTWYHHGKRWKNKFTLVVGIIHTNYLEYVKREKNGAVEAFLLEYINRWVVHIYC 181
           LEEPEHL WY+HGKRW +KF  VVGI+HTNYLEY+KREKNGA++AF + ++N WV   YC
Sbjct: 461 LEEPEHLNWYYHGKRWTDKFNHVVGIVHTNYLEYIKREKNGALQAFFVNHVNNWVTRAYC 520

Query: 182 HKVIRLSAATQDYPKSIICNVHGVNPKFLEIGKKKMEQQQNGGQVFGKGAYYIGKMIWSK 241
            KV+RLSAATQD PKS++CNVHGVNPKFL IG+K  E++  G Q F KGAY++GKM+W+K
Sbjct: 521 DKVLRLSAATQDLPKSVVCNVHGVNPKFLMIGEKIAEERSRGEQAFSKGAYFLGKMVWAK 580

Query: 242 GYRELVKLLSDYQKDFAELEVDLFGNGEDSDEVQKATQDLEVSVKVHPGRDHTDPIFH 300
           GYREL+ L++ ++ +     +D++GNGED+ EVQ+A +  ++++    GRDH D   H
Sbjct: 581 GYRELIDLMAKHKSELGSFNLDVYGNGEDAVEVQRAAKKHDLNLNFLKGRDHADDALH 638

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6DW753.4e-20573.49Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Glycine max OX=3847 GN=D... [more]
Q6DW732.2e-20473.06Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Lotus japonicus OX=34305... [more]
Q8W1S14.4e-19266.17Digalactosyldiacylglycerol synthase 2, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q6DW763.4e-16057.48Digalactosyldiacylglycerol synthase 1, chloroplastic OS=Glycine max OX=3847 GN=D... [more]
Q6DW745.0e-15656.41Digalactosyldiacylglycerol synthase 1, chloroplastic OS=Lotus japonicus OX=34305... [more]
Match NameE-valueIdentityDescription
A0A0A0L6B24.6e-277100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122630 PE=3 SV=1[more]
A0A1S3AV601.8e-26594.88digalactosyldiacylglycerol synthase 2, chloroplastic OS=Cucumis melo OX=3656 GN=... [more]
A0A5D3D3K61.8e-26594.88Digalactosyldiacylglycerol synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1D1F64.0e-24989.34digalactosyldiacylglycerol synthase 2, chloroplastic OS=Momordica charantia OX=3... [more]
A0A6J1IFT47.9e-24587.42digalactosyldiacylglycerol synthase 2, chloroplastic OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
XP_004133875.19.5e-277100.00digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis sativus] >KGN56539... [more]
XP_008438079.13.7e-26594.88PREDICTED: digalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis melo] >... [more]
XP_038878183.12.3e-25491.47digalactosyldiacylglycerol synthase 2, chloroplastic [Benincasa hispida][more]
XP_022147482.18.3e-24989.34digalactosyldiacylglycerol synthase 2, chloroplastic [Momordica charantia] >XP_0... [more]
XP_023540326.13.3e-24587.42digalactosyldiacylglycerol synthase 2, chloroplastic isoform X1 [Cucurbita pepo ... [more]
Match NameE-valueIdentityDescription
AT4G00550.13.1e-19366.17digalactosyl diacylglycerol deficient 2 [more]
AT3G11670.19.8e-15555.05UDP-Glycosyltransferase superfamily protein [more]
AT3G11670.27.8e-11259.40UDP-Glycosyltransferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 243..263
NoneNo IPR availablePFAMPF13692Glyco_trans_1_4coord: 232..360
e-value: 2.4E-9
score: 37.7
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 230..402
e-value: 3.9E-17
score: 64.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 443..469
NoneNo IPR availablePANTHERPTHR46132:SF1DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTICcoord: 2..460
NoneNo IPR availableCDDcd01635Glycosyltransferase_GTB-typecoord: 166..347
e-value: 1.43321E-17
score: 79.7556
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 7..387
IPR044525Digalactosyldiacylglycerol synthase 1/2PANTHERPTHR46132DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTICcoord: 2..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G09010.1CSPI03G09010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019375 galactolipid biosynthetic process
cellular_component GO:0009707 chloroplast outer membrane
molecular_function GO:0046481 digalactosyldiacylglycerol synthase activity