CSPI03G08490 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G08490
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionWRKY transcription factor 22
LocationChr3: 7096594 .. 7098572 (-)
RNA-Seq ExpressionCSPI03G08490
SyntenyCSPI03G08490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTTTCTATATATAATTCTTTTCCTACTCATTACTCATTACACCCTTATCACCAAAAGTCAGCGCCTTCTTTTGTCTCTATTTTTCTCTCTTAGAACAAAACAACCAAAAAAGAAGAAAGAAAAAAAAGAAACTGATCAAAGAGAAGAAGTTGAGAGAGACAAAGGTACAGATTCTCAGAGCAGAGTTCTGTCTTTTGATAATGGAAGTCGATTGGGATCTTCATGCCGTCGTCAGAGGTTACTCCGCCGCGCCCTCTGCTGCTACCATTGTTCCCGCTTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCAATAACCCTGTCGTCCCTTTTTCTTTTGGTAGAGACCTAACCAACAATCAAATGAAAAACCATTTCTTCTCTCTTCAAGATCCATTTCAACCCTCTAATTGTAATTCCACTCAAGAATTGCATGAGCTTTTCAAACCCTTTTTCCCTAAATCTCAACCATCTCCTTCTCCTCCGCCGCCTCCTCCTGCACCGCCTGCTCCGTCGCTTCTTTCTTCTCCTCCGGCTCCTAAAATTTTGACTCATCAGAAACAGAGCACCCATCTCCCTAAACAACTCCATTCAACCTCTGCTTCTGCTCCGAGATCCAAACGCCGGTGAGCAAGATTCTTCCATTTCTTCTTCATTTTGGATGTGTGTTTGTTTGTTTGTTTGTATTTCTTTTAGCTAATCGATCTTTTGTTTTGGTTCTCTTTTTAGGAAGAATCAGCTGAAGAAGGTTTGTCAAGTTCCGGCGGAGTCTCTGTCTTCGGATATTTGGGCTTGGCGAAAATATGGACAAAAACCCATTAAAGGATCTCCATATCCAAGGTTTGTGCTCAATTTCAATTCTGAGATTAGGGTTTTTTAGGGTTTTGATTTTGGGGTTAGAACTAATTTTTGGTGCTTTTTTAAAAAATAACAGGGGATATTATAGATGTAGCAGTTCGAAGGGTTGCATGGCCCGAAAACAAGTGGAGAGGAACAGATCCGACCCGGGAATGTTCATAGTCACTTACACGGCGGAGCACAACCACCCGGCGCCTACTCACCGCAACTCTCTCGCCGGCTCCACCCGTCAGAAACCCATCACACCGTCCACCACCGCGTCCGGATCTGAAAAACTCGACCCGAAACAACCGGTCTGCTCGTCGGAAGAACAAAGTACGATCACGGAGAGCAAAGAAGAGAAAGAGGAATTATTAATGGCCGAAGATGAAGAAGACGACGATCTGGGAATCTCCGATTTGATCGTGAACGACGATTTCTATGTGGGTTTTGAAGAACTTGACAGCCCAATAACCGACGACTGCTTTTCCGATCCATTCCCGGCCAACTTCGACCTTCCATGGTTGTTTAACGGAAATCCAGACGGCGAAATTGTAAAACTCCCTTGTTAAATTAATTTCCAACTTTTTTTTTTTATAATCATTCTCCAAACAGAGATCTGTAAAATCAAAGATGTTAGAGCAAAGATTTTAACAGTGTTTTTAGAAAGAGAAAGAGCGAGCAAGATAGTAGTAAATTTCGTCTTGTTTTCTCTGTAATTTTAGAAGAACAGTGATATTGAAGTTGAAGATGCAGTGGGTTTTTGTGAGAATTTTTTTAGGGAAAAAGGAATGAACAACATAAGGGGGGGTTGACTAATTAAAAGAAAAGAAGTGGAATCAAGCAATGAATGAATGACGTGTAGATGGCGTTGACAGAGATGGAAAATTAGAAGAGAGATTAAAAGCAGAGGTTGAAGAAGAGAAAATGGGTATGGAGGAGGAAGATTAAGAATATTGGCTTCTTTTTTCCAAAATGCTTGTCCGAGGACAACAATGGTGGTGGTGGTGATGGTGGAGAATCAGATTTTTTCTTTTGTTTATAGATGAAGAAAAAAAGAAAAAAAAAGAAAAAAAAGAGGATTTGGAATTTTTCATTGTAGTTGATGCCAGTTGTGGTCATAGTGGTAAAGATAAATGGTA

mRNA sequence

GGTTTCTATATATAATTCTTTTCCTACTCATTACTCATTACACCCTTATCACCAAAAGTCAGCGCCTTCTTTTGTCTCTATTTTTCTCTCTTAGAACAAAACAACCAAAAAAGAAGAAAGAAAAAAAAGAAACTGATCAAAGAGAAGAAGTTGAGAGAGACAAAGGTACAGATTCTCAGAGCAGAGTTCTGTCTTTTGATAATGGAAGTCGATTGGGATCTTCATGCCGTCGTCAGAGGTTACTCCGCCGCGCCCTCTGCTGCTACCATTGTTCCCGCTTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCAATAACCCTGTCGTCCCTTTTTCTTTTGGTAGAGACCTAACCAACAATCAAATGAAAAACCATTTCTTCTCTCTTCAAGATCCATTTCAACCCTCTAATTGTAATTCCACTCAAGAATTGCATGAGCTTTTCAAACCCTTTTTCCCTAAATCTCAACCATCTCCTTCTCCTCCGCCGCCTCCTCCTGCACCGCCTGCTCCGTCGCTTCTTTCTTCTCCTCCGGCTCCTAAAATTTTGACTCATCAGAAACAGAGCACCCATCTCCCTAAACAACTCCATTCAACCTCTGCTTCTGCTCCGAGATCCAAACGCCGGAAGAATCAGCTGAAGAAGGTTTGTCAAGTTCCGGCGGAGTCTCTGTCTTCGGATATTTGGGCTTGGCGAAAATATGGACAAAAACCCATTAAAGGATCTCCATATCCAAGGGGATATTATAGATGTAGCAGTTCGAAGGGTTGCATGGCCCGAAAACAAGTGGAGAGGAACAGATCCGACCCGGGAATGTTCATAGTCACTTACACGGCGGAGCACAACCACCCGGCGCCTACTCACCGCAACTCTCTCGCCGGCTCCACCCGTCAGAAACCCATCACACCGTCCACCACCGCGTCCGGATCTGAAAAACTCGACCCGAAACAACCGGTCTGCTCGTCGGAAGAACAAAGTACGATCACGGAGAGCAAAGAAGAGAAAGAGGAATTATTAATGGCCGAAGATGAAGAAGACGACGATCTGGGAATCTCCGATTTGATCGTGAACGACGATTTCTATGTGGGTTTTGAAGAACTTGACAGCCCAATAACCGACGACTGCTTTTCCGATCCATTCCCGGCCAACTTCGACCTTCCATGGTTGTTTAACGGAAATCCAGACGGCGAAATTGTAAAACTCCCTTGTTAAATTAATTTCCAACTTTTTTTTTTTATAATCATTCTCCAAACAGAGATCTGTAAAATCAAAGATGTTAGAGCAAAGATTTTAACAGTGTTTTTAGAAAGAGAAAGAGCGAGCAAGATAGTAGTAAATTTCGTCTTGTTTTCTCTGTAATTTTAGAAGAACAGTGATATTGAAGTTGAAGATGCAGTGGGTTTTTGTGAGAATTTTTTTAGGGAAAAAGGAATGAACAACATAAGGGGGGGTTGACTAATTAAAAGAAAAGAAGTGGAATCAAGCAATGAATGAATGACGTGTAGATGGCGTTGACAGAGATGGAAAATTAGAAGAGAGATTAAAAGCAGAGGTTGAAGAAGAGAAAATGGGTATGGAGGAGGAAGATTAAGAATATTGGCTTCTTTTTTCCAAAATGCTTGTCCGAGGACAACAATGGTGGTGGTGGTGATGGTGGAGAATCAGATTTTTTCTTTTGTTTATAGATGAAGAAAAAAAGAAAAAAAAAGAAAAAAAAGAGGATTTGGAATTTTTCATTGTAGTTGATGCCAGTTGTGGTCATAGTGGTAAAGATAAATGGTA

Coding sequence (CDS)

ATGGAAGTCGATTGGGATCTTCATGCCGTCGTCAGAGGTTACTCCGCCGCGCCCTCTGCTGCTACCATTGTTCCCGCTTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCAATAACCCTGTCGTCCCTTTTTCTTTTGGTAGAGACCTAACCAACAATCAAATGAAAAACCATTTCTTCTCTCTTCAAGATCCATTTCAACCCTCTAATTGTAATTCCACTCAAGAATTGCATGAGCTTTTCAAACCCTTTTTCCCTAAATCTCAACCATCTCCTTCTCCTCCGCCGCCTCCTCCTGCACCGCCTGCTCCGTCGCTTCTTTCTTCTCCTCCGGCTCCTAAAATTTTGACTCATCAGAAACAGAGCACCCATCTCCCTAAACAACTCCATTCAACCTCTGCTTCTGCTCCGAGATCCAAACGCCGGAAGAATCAGCTGAAGAAGGTTTGTCAAGTTCCGGCGGAGTCTCTGTCTTCGGATATTTGGGCTTGGCGAAAATATGGACAAAAACCCATTAAAGGATCTCCATATCCAAGGGGATATTATAGATGTAGCAGTTCGAAGGGTTGCATGGCCCGAAAACAAGTGGAGAGGAACAGATCCGACCCGGGAATGTTCATAGTCACTTACACGGCGGAGCACAACCACCCGGCGCCTACTCACCGCAACTCTCTCGCCGGCTCCACCCGTCAGAAACCCATCACACCGTCCACCACCGCGTCCGGATCTGAAAAACTCGACCCGAAACAACCGGTCTGCTCGTCGGAAGAACAAAGTACGATCACGGAGAGCAAAGAAGAGAAAGAGGAATTATTAATGGCCGAAGATGAAGAAGACGACGATCTGGGAATCTCCGATTTGATCGTGAACGACGATTTCTATGTGGGTTTTGAAGAACTTGACAGCCCAATAACCGACGACTGCTTTTCCGATCCATTCCCGGCCAACTTCGACCTTCCATGGTTGTTTAACGGAAATCCAGACGGCGAAATTGTAAAACTCCCTTGTTAA

Protein sequence

MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC*
Homology
BLAST of CSPI03G08490 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 8.6e-57
Identity = 157/332 (47.29%), Postives = 194/332 (58.43%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           M  DWDLHAVVRG SA  S+AT    ++  S   SS  NP+  F+ GR            
Sbjct: 1   MADDWDLHAVVRGCSAVSSSAT----TTVYSPGVSSHTNPI--FTVGRQ----------- 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
                   SN  S  E+ +L+ PF  +S  S           + S ++ P  P+   +QK
Sbjct: 61  --------SNAVSFGEIRDLYTPFTQESVVS-----------SFSCINYPEEPRKPQNQK 120

Query: 121 QSTHLPKQLHS-----TSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGS 180
           +   L     S     + ++  RSKRRK Q KKVC V AE+L+SD+WAWRKYGQKPIKGS
Sbjct: 121 RPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAAEALNSDVWAWRKYGQKPIKGS 180

Query: 181 PYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPIT 240
           PYPRGYYRCS+SKGC+ARKQVERNRSDP MFIVTYTAEHNHPAPTHRNSLAGSTRQKP  
Sbjct: 181 PYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTRQKPSD 240

Query: 241 PSTTASGSEKLD--PKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDF 300
             T+ S +  +      PV S++E     E      +L    D E+D L +SD +V+DDF
Sbjct: 241 QQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDL----DGEEDLLSLSDTVVSDDF 289

Query: 301 YVGFEELDSPITDDCFS-DPFPANFDLPWLFN 325
           + G EE       D FS +  PA+FDL W+ N
Sbjct: 301 FDGLEEF---AAGDSFSGNSAPASFDLSWVVN 289

BLAST of CSPI03G08490 vs. ExPASy Swiss-Prot
Match: Q9FLX8 (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.6e-39
Identity = 131/338 (38.76%), Postives = 167/338 (49.41%), Query Frame = 0

Query: 4   DWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQ 63
           DWDL AVVR  S++ S             +     +P  P  F                 
Sbjct: 5   DWDLFAVVRSCSSSVSTTNSCAGHEDDIGNCKQQQDPPPPPLFQAS-------------- 64

Query: 64  DPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQKQ-- 123
                S+CN   EL +  KPF P +  + +   PPP  P P   +S P+P IL  Q+Q  
Sbjct: 65  -----SSCN---ELQDSCKPFLPVTTTTTTTWSPPPLLPPPK--ASSPSPNILLKQEQVL 124

Query: 124 -----------------ST---------HLPKQLHSTSASAPRSKRRKNQLKK-VCQVPA 183
                            ST            + L   S    RS++RKNQ K+ +C V  
Sbjct: 125 LESQDQKPPLSVRVFPPSTSSSVFVFRGQRDQLLQQQSQPPLRSRKRKNQQKRTICHVTQ 184

Query: 184 ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEH 243
           E+LSSD+WAWRKYGQKPIKGSPYPR YYRCSSSKGC+ARKQVER+  DP +FIVTYT EH
Sbjct: 185 ENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEH 244

Query: 244 NHPAPTHRNSLAGSTRQK--PITP---------STTASGSEKLDPKQPVCSSEE------ 290
            HP PTHRNSLAGSTR K  P+ P         S T      L P  P+  +++      
Sbjct: 245 THPRPTHRNSLAGSTRNKSQPVNPVPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNG 304

BLAST of CSPI03G08490 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 7.8e-34
Identity = 79/150 (52.67%), Postives = 102/150 (68.00%), Query Frame = 0

Query: 134 ASAPRS---KRRKNQLKKVCQVPA----------ESLSSDIWAWRKYGQKPIKGSPYPRG 193
           +S+PR+   KRRK+Q KKV  +PA          E + SD+WAWRKYGQKPIKGSPYPRG
Sbjct: 177 SSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPYPRG 236

Query: 194 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 253
           YYRCSSSKGC ARKQVER+R+DP M ++TYT+EHNHP PT RN+LAGSTR      S+++
Sbjct: 237 YYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRS-----SSSS 296

Query: 254 SGSEKLDPKQPVCSSEEQSTITESKEEKEE 271
           S +          ++   S + ++   K+E
Sbjct: 297 SLNPSSKSSTAAATTSPSSRVFQNNSSKDE 321

BLAST of CSPI03G08490 vs. ExPASy Swiss-Prot
Match: Q9SUS1 (Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.6e-29
Identity = 108/275 (39.27%), Postives = 142/275 (51.64%), Query Frame = 0

Query: 6   DLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQDP 65
           DL A+VRGYS +  A     +  SS + S S   P+   SF                   
Sbjct: 5   DLEAIVRGYSGSGDAF----SGESSGTFSPSFCLPMETSSF------------------- 64

Query: 66  FQPS-NCNSTQELHELFKPFFPKSQP---SPSPPPPPPAPPAPSLLSSPPAPKILTHQKQ 125
           ++P    +   EL EL+KPF+P S     + S   P  + P            +L++  +
Sbjct: 65  YEPEMETSGLDELGELYKPFYPFSTQTILTSSVSLPEDSKPFRDDKKQRSHGCLLSNGSR 124

Query: 126 STHLPKQLHSTSASAPRSKRRKNQLKKVC-QVPAESLSSDIWAWRKYGQKPIKGSPYPRG 185
           + H+        + +   K +KNQ K+V  QV  E+L SD WAWRKYGQKPIKGSPYPR 
Sbjct: 125 ADHI------RISESKSKKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRS 184

Query: 186 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 245
           YYRCSSSKGC+ARKQVERN  +P  F +TYT EHNH  PT RNSLAGSTR K   P  T 
Sbjct: 185 YYRCSSSKGCLARKQVERNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTL 244

Query: 246 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAE 276
           +   + +      S+    +  ES    +E+ +AE
Sbjct: 245 TKKSEKEVVSSPTSNPMIPSADESSVAVQEMSVAE 250

BLAST of CSPI03G08490 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 6.4e-28
Identity = 109/300 (36.33%), Postives = 149/300 (49.67%), Query Frame = 0

Query: 44  FSFGRDLTNNQMKNHFFSLQDPFQ----PSNCNSTQELHELFKPFFPKSQPSPSPPPPPP 103
           FS   D  NN   N+ F +   F+     S C+    +          S P  SP     
Sbjct: 82  FSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQCSIFPRIRISHSNIIHDSSPCNSPAMSAH 141

Query: 104 APPAPSLLSSPPAPKILTHQKQS------THLPKQLHSTSASAPRSKRRKNQLKKVCQVP 163
              A +  S      + T+  ++      T    Q+  +S      KRRK+Q KKV  +P
Sbjct: 142 VVAAAAAASPRGIINVDTNSPRNCLLVDGTTFSSQIQISSPRNLGLKRRKSQAKKVVCIP 201

Query: 164 A----------ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDP 223
           A          E + SD+WAWRKYGQKPIKGSP+PRGYYRCSSSKGC ARKQVER+R+DP
Sbjct: 202 APAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPFPRGYYRCSSSKGCSARKQVERSRTDP 261

Query: 224 GMFIVTYTAEHNHPAPTHRNSLAGSTRQ--------KPITPSTTASGSEKLDPKQPV--- 283
            M ++TYT+EHNHP P  RN+LAGSTR          P  PST    S  +  +  +   
Sbjct: 262 NMLVITYTSEHNHPWPIQRNALAGSTRSSTSSSSNPNPSKPSTANVNSSSIGSQNTIYLP 321

Query: 284 CSSEEQSTITES--KEEK-EELLMAEDEEDDDLGISDLIVN--------DDFYVGFEELD 302
            S+    T++ S  K+E+ +++ +   ++DDD  I+             DDF+   EEL+
Sbjct: 322 SSTTPPPTLSSSAIKDERGDDMELENVDDDDDNQIAPYRPELHDHQHQPDDFFADLEELE 381

BLAST of CSPI03G08490 vs. ExPASy TrEMBL
Match: E7CEW7 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY18 PE=2 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 4.6e-186
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120

Query: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180
           QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG
Sbjct: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180

Query: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240
           YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA
Sbjct: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240

Query: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300
           SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL
Sbjct: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300

Query: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 337
           DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336

BLAST of CSPI03G08490 vs. ExPASy TrEMBL
Match: A0A0A0L7C1 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121580 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.5e-184
Identity = 335/336 (99.70%), Postives = 335/336 (99.70%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVPA SSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPA-SSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120

Query: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180
           QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG
Sbjct: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180

Query: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240
           YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA
Sbjct: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240

Query: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300
           SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL
Sbjct: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300

Query: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 337
           DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 335

BLAST of CSPI03G08490 vs. ExPASy TrEMBL
Match: A0A5D3D3F9 (WRKY transcription factor 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004970 PE=4 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 4.6e-170
Identity = 314/339 (92.63%), Postives = 321/339 (94.69%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVP+SSSS S++S      VPFSFGRDLTNNQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSSFSNNS------VPFSFGRDLTNNQTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQP---SPSPPPPPPAPPAPSLLSSPPAPKILT 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQP   SP+PPPPPP P AP LLSS PAPKILT
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSS-PAPKILT 120

Query: 121 HQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180
           H KQSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY
Sbjct: 121 HHKQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180

Query: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPS 240
           PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TP+
Sbjct: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPT 240

Query: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGF 300
           TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGF
Sbjct: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGF 300

Query: 301 EELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 337
           EELDSPITDDCFSD FPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 EELDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 332

BLAST of CSPI03G08490 vs. ExPASy TrEMBL
Match: A0A1S3AVV5 (WRKY transcription factor 22 OS=Cucumis melo OX=3656 GN=LOC103483243 PE=4 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.3e-169
Identity = 313/339 (92.33%), Postives = 320/339 (94.40%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVP+SSS S++S       VPFSFGRDLTNNQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSFSNNS-------VPFSFGRDLTNNQTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQP---SPSPPPPPPAPPAPSLLSSPPAPKILT 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQP   SP+PPPPPP P AP LLSS PAPKILT
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSS-PAPKILT 120

Query: 121 HQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180
           H KQSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY
Sbjct: 121 HHKQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180

Query: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPS 240
           PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TP+
Sbjct: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPT 240

Query: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGF 300
           TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGF
Sbjct: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGF 300

Query: 301 EELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 337
           EELDSPITDDCFSD FPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 EELDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 331

BLAST of CSPI03G08490 vs. ExPASy TrEMBL
Match: A0A6J1EEC7 (WRKY transcription factor 22 OS=Cucurbita moschata OX=3662 GN=LOC111431800 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 8.4e-140
Identity = 276/337 (81.90%), Postives = 294/337 (87.24%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSA  SAATI P+S   S SS+S+N+   PF+FGRDLTN    NHFF
Sbjct: 3   MEVDWDLHAVVRGYSAVSSAATI-PSSDFYSVSSTSNNS--TPFAFGRDLTN--QTNHFF 62

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPF+  NCNST+ELHELFKPF PK  P PSPPPPPP+ P   LL S PA KILTHQK
Sbjct: 63  SLQDPFEGPNCNSTEELHELFKPFSPK--PLPSPPPPPPSAP---LLLSSPAAKILTHQK 122

Query: 121 --QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180
             Q+TH+PKQLHS   SA RSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP
Sbjct: 123 QRQNTHVPKQLHSAPVSASRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 182

Query: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPST 240
           RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP T + 
Sbjct: 183 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPNTQTP 242

Query: 241 TASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFE 300
            +SGSEK D KQPVCSSE+QSTITESKEEKEELL AEDEEDDDLG+SDLIVNDDFY+GFE
Sbjct: 243 ASSGSEKPDSKQPVCSSEDQSTITESKEEKEELL-AEDEEDDDLGVSDLIVNDDFYMGFE 302

Query: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLP 336
           ELDSPITD+CFSD FPANF+LPW FNG+PD EIVKLP
Sbjct: 303 ELDSPITDECFSDQFPANFELPWSFNGDPDSEIVKLP 328

BLAST of CSPI03G08490 vs. NCBI nr
Match: NP_001267594.1 (WRKY transcription factor 22-like [Cucumis sativus] >ADU52510.1 WRKY protein [Cucumis sativus] >KAE8650243.1 hypothetical protein Csa_011009 [Cucumis sativus])

HSP 1 Score: 660.2 bits (1702), Expect = 9.4e-186
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120

Query: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180
           QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG
Sbjct: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180

Query: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240
           YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA
Sbjct: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240

Query: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300
           SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL
Sbjct: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300

Query: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 337
           DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336

BLAST of CSPI03G08490 vs. NCBI nr
Match: TYK17626.1 (WRKY transcription factor 22 [Cucumis melo var. makuwa])

HSP 1 Score: 607.1 bits (1564), Expect = 9.5e-170
Identity = 314/339 (92.63%), Postives = 321/339 (94.69%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVP+SSSS S++S      VPFSFGRDLTNNQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSSFSNNS------VPFSFGRDLTNNQTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQP---SPSPPPPPPAPPAPSLLSSPPAPKILT 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQP   SP+PPPPPP P AP LLSS PAPKILT
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSS-PAPKILT 120

Query: 121 HQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180
           H KQSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY
Sbjct: 121 HHKQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180

Query: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPS 240
           PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TP+
Sbjct: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPT 240

Query: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGF 300
           TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGF
Sbjct: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGF 300

Query: 301 EELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 337
           EELDSPITDDCFSD FPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 EELDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 332

BLAST of CSPI03G08490 vs. NCBI nr
Match: XP_008437986.1 (PREDICTED: WRKY transcription factor 22 [Cucumis melo])

HSP 1 Score: 605.5 bits (1560), Expect = 2.8e-169
Identity = 313/339 (92.33%), Postives = 320/339 (94.40%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVP+SSS S++S       VPFSFGRDLTNNQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSFSNNS-------VPFSFGRDLTNNQTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQP---SPSPPPPPPAPPAPSLLSSPPAPKILT 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQP   SP+PPPPPP P AP LLSS PAPKILT
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSS-PAPKILT 120

Query: 121 HQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180
           H KQSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY
Sbjct: 121 HHKQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180

Query: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPS 240
           PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TP+
Sbjct: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPT 240

Query: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGF 300
           TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGF
Sbjct: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGF 300

Query: 301 EELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 337
           EELDSPITDDCFSD FPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 EELDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 331

BLAST of CSPI03G08490 vs. NCBI nr
Match: XP_038880705.1 (WRKY transcription factor 22 [Benincasa hispida])

HSP 1 Score: 573.9 bits (1478), Expect = 8.9e-160
Identity = 301/338 (89.05%), Postives = 309/338 (91.42%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSA  S ATI+P+SSSSSS++S      +PFSF RDLT NQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAVSSTATIIPSSSSSSSNNS------IPFSFSRDLT-NQTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILT--H 120
           SLQDPFQP NCNSTQELHELFKPFFPKSQPSP PPPPPPAPP    L S PA KILT   
Sbjct: 61  SLQDPFQPPNCNSTQELHELFKPFFPKSQPSPPPPPPPPAPP----LLSSPAAKILTPPK 120

Query: 121 QKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180
           Q+QSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP
Sbjct: 121 QQQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180

Query: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPST 240
           RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITP+ 
Sbjct: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTP 240

Query: 241 TASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFE 300
           TASGS K DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGFE
Sbjct: 241 TASGSGKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFE 300

Query: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 337
           ELDSPITDDCFSDPFPANFDLPWLFNGNPD EIVKLPC
Sbjct: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDSEIVKLPC 327

BLAST of CSPI03G08490 vs. NCBI nr
Match: KAG6582635.1 (Proline iminopeptidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 510.0 bits (1312), Expect = 1.6e-140
Identity = 277/338 (81.95%), Postives = 295/338 (87.28%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSA  SAATI P+S   S SS+S+N+   PF+FGRDLTN    NHFF
Sbjct: 3   MEVDWDLHAVVRGYSAVSSAATI-PSSDFYSVSSTSNNS--TPFAFGRDLTN--QTNHFF 62

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPF+  NCNST+ELHELFKPF PK  P PSPPPPPP+ P   LL S PA KILTHQK
Sbjct: 63  SLQDPFEGPNCNSTEELHELFKPFSPK--PLPSPPPPPPSAP---LLLSSPAAKILTHQK 122

Query: 121 --QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180
             Q+TH+PKQLHS   SA RSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP
Sbjct: 123 QRQNTHVPKQLHSVPVSASRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 182

Query: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPST 240
           RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP T + 
Sbjct: 183 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPNTQTP 242

Query: 241 TASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFE 300
            +SGSEK D KQPVCSSE+QSTITESKEEKEELL AEDEEDDDLG+SDLIVNDDFY+GFE
Sbjct: 243 ASSGSEKPDSKQPVCSSEDQSTITESKEEKEELL-AEDEEDDDLGVSDLIVNDDFYMGFE 302

Query: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 337
           ELDSPITD+CFSD FPANF+LPW FNG+PD EIVKLPC
Sbjct: 303 ELDSPITDECFSDQFPANFELPWSFNGDPDSEIVKLPC 329

BLAST of CSPI03G08490 vs. TAIR 10
Match: AT4G01250.1 (WRKY family transcription factor )

HSP 1 Score: 222.2 bits (565), Expect = 6.1e-58
Identity = 157/332 (47.29%), Postives = 194/332 (58.43%), Query Frame = 0

Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           M  DWDLHAVVRG SA  S+AT    ++  S   SS  NP+  F+ GR            
Sbjct: 1   MADDWDLHAVVRGCSAVSSSAT----TTVYSPGVSSHTNPI--FTVGRQ----------- 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
                   SN  S  E+ +L+ PF  +S  S           + S ++ P  P+   +QK
Sbjct: 61  --------SNAVSFGEIRDLYTPFTQESVVS-----------SFSCINYPEEPRKPQNQK 120

Query: 121 QSTHLPKQLHS-----TSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGS 180
           +   L     S     + ++  RSKRRK Q KKVC V AE+L+SD+WAWRKYGQKPIKGS
Sbjct: 121 RPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAAEALNSDVWAWRKYGQKPIKGS 180

Query: 181 PYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPIT 240
           PYPRGYYRCS+SKGC+ARKQVERNRSDP MFIVTYTAEHNHPAPTHRNSLAGSTRQKP  
Sbjct: 181 PYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTRQKPSD 240

Query: 241 PSTTASGSEKLD--PKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDF 300
             T+ S +  +      PV S++E     E      +L    D E+D L +SD +V+DDF
Sbjct: 241 QQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDL----DGEEDLLSLSDTVVSDDF 289

Query: 301 YVGFEELDSPITDDCFS-DPFPANFDLPWLFN 325
           + G EE       D FS +  PA+FDL W+ N
Sbjct: 301 FDGLEEF---AAGDSFSGNSAPASFDLSWVVN 289

BLAST of CSPI03G08490 vs. TAIR 10
Match: AT5G52830.1 (WRKY DNA-binding protein 27 )

HSP 1 Score: 163.7 bits (413), Expect = 2.6e-40
Identity = 131/338 (38.76%), Postives = 167/338 (49.41%), Query Frame = 0

Query: 4   DWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQ 63
           DWDL AVVR  S++ S             +     +P  P  F                 
Sbjct: 5   DWDLFAVVRSCSSSVSTTNSCAGHEDDIGNCKQQQDPPPPPLFQAS-------------- 64

Query: 64  DPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQKQ-- 123
                S+CN   EL +  KPF P +  + +   PPP  P P   +S P+P IL  Q+Q  
Sbjct: 65  -----SSCN---ELQDSCKPFLPVTTTTTTTWSPPPLLPPPK--ASSPSPNILLKQEQVL 124

Query: 124 -----------------ST---------HLPKQLHSTSASAPRSKRRKNQLKK-VCQVPA 183
                            ST            + L   S    RS++RKNQ K+ +C V  
Sbjct: 125 LESQDQKPPLSVRVFPPSTSSSVFVFRGQRDQLLQQQSQPPLRSRKRKNQQKRTICHVTQ 184

Query: 184 ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEH 243
           E+LSSD+WAWRKYGQKPIKGSPYPR YYRCSSSKGC+ARKQVER+  DP +FIVTYT EH
Sbjct: 185 ENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEH 244

Query: 244 NHPAPTHRNSLAGSTRQK--PITP---------STTASGSEKLDPKQPVCSSEE------ 290
            HP PTHRNSLAGSTR K  P+ P         S T      L P  P+  +++      
Sbjct: 245 THPRPTHRNSLAGSTRNKSQPVNPVPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNG 304

BLAST of CSPI03G08490 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 146.0 bits (367), Expect = 5.6e-35
Identity = 79/150 (52.67%), Postives = 102/150 (68.00%), Query Frame = 0

Query: 134 ASAPRS---KRRKNQLKKVCQVPA----------ESLSSDIWAWRKYGQKPIKGSPYPRG 193
           +S+PR+   KRRK+Q KKV  +PA          E + SD+WAWRKYGQKPIKGSPYPRG
Sbjct: 177 SSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPYPRG 236

Query: 194 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 253
           YYRCSSSKGC ARKQVER+R+DP M ++TYT+EHNHP PT RN+LAGSTR      S+++
Sbjct: 237 YYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRS-----SSSS 296

Query: 254 SGSEKLDPKQPVCSSEEQSTITESKEEKEE 271
           S +          ++   S + ++   K+E
Sbjct: 297 SLNPSSKSSTAAATTSPSSRVFQNNSSKDE 321

BLAST of CSPI03G08490 vs. TAIR 10
Match: AT4G23550.1 (WRKY family transcription factor )

HSP 1 Score: 129.4 bits (324), Expect = 5.4e-30
Identity = 108/275 (39.27%), Postives = 142/275 (51.64%), Query Frame = 0

Query: 6   DLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQDP 65
           DL A+VRGYS +  A     +  SS + S S   P+   SF                   
Sbjct: 5   DLEAIVRGYSGSGDAF----SGESSGTFSPSFCLPMETSSF------------------- 64

Query: 66  FQPS-NCNSTQELHELFKPFFPKSQP---SPSPPPPPPAPPAPSLLSSPPAPKILTHQKQ 125
           ++P    +   EL EL+KPF+P S     + S   P  + P            +L++  +
Sbjct: 65  YEPEMETSGLDELGELYKPFYPFSTQTILTSSVSLPEDSKPFRDDKKQRSHGCLLSNGSR 124

Query: 126 STHLPKQLHSTSASAPRSKRRKNQLKKVC-QVPAESLSSDIWAWRKYGQKPIKGSPYPRG 185
           + H+        + +   K +KNQ K+V  QV  E+L SD WAWRKYGQKPIKGSPYPR 
Sbjct: 125 ADHI------RISESKSKKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRS 184

Query: 186 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 245
           YYRCSSSKGC+ARKQVERN  +P  F +TYT EHNH  PT RNSLAGSTR K   P  T 
Sbjct: 185 YYRCSSSKGCLARKQVERNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTL 244

Query: 246 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAE 276
           +   + +      S+    +  ES    +E+ +AE
Sbjct: 245 TKKSEKEVVSSPTSNPMIPSADESSVAVQEMSVAE 250

BLAST of CSPI03G08490 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 126.3 bits (316), Expect = 4.6e-29
Identity = 109/300 (36.33%), Postives = 149/300 (49.67%), Query Frame = 0

Query: 44  FSFGRDLTNNQMKNHFFSLQDPFQ----PSNCNSTQELHELFKPFFPKSQPSPSPPPPPP 103
           FS   D  NN   N+ F +   F+     S C+    +          S P  SP     
Sbjct: 82  FSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQCSIFPRIRISHSNIIHDSSPCNSPAMSAH 141

Query: 104 APPAPSLLSSPPAPKILTHQKQS------THLPKQLHSTSASAPRSKRRKNQLKKVCQVP 163
              A +  S      + T+  ++      T    Q+  +S      KRRK+Q KKV  +P
Sbjct: 142 VVAAAAAASPRGIINVDTNSPRNCLLVDGTTFSSQIQISSPRNLGLKRRKSQAKKVVCIP 201

Query: 164 A----------ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDP 223
           A          E + SD+WAWRKYGQKPIKGSP+PRGYYRCSSSKGC ARKQVER+R+DP
Sbjct: 202 APAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPFPRGYYRCSSSKGCSARKQVERSRTDP 261

Query: 224 GMFIVTYTAEHNHPAPTHRNSLAGSTRQ--------KPITPSTTASGSEKLDPKQPV--- 283
            M ++TYT+EHNHP P  RN+LAGSTR          P  PST    S  +  +  +   
Sbjct: 262 NMLVITYTSEHNHPWPIQRNALAGSTRSSTSSSSNPNPSKPSTANVNSSSIGSQNTIYLP 321

Query: 284 CSSEEQSTITES--KEEK-EELLMAEDEEDDDLGISDLIVN--------DDFYVGFEELD 302
            S+    T++ S  K+E+ +++ +   ++DDD  I+             DDF+   EEL+
Sbjct: 322 SSTTPPPTLSSSAIKDERGDDMELENVDDDDDNQIAPYRPELHDHQHQPDDFFADLEELE 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O046098.6e-5747.29WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1[more]
Q9FLX83.6e-3938.76Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 ... [more]
O647477.8e-3452.67Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
Q9SUS17.6e-2939.27Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 ... [more]
Q9SA806.4e-2836.33Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ... [more]
Match NameE-valueIdentityDescription
E7CEW74.6e-186100.00WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY18 PE=2 SV=1[more]
A0A0A0L7C12.5e-18499.70WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121580 PE=4 S... [more]
A0A5D3D3F94.6e-17092.63WRKY transcription factor 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3AVV51.3e-16992.33WRKY transcription factor 22 OS=Cucumis melo OX=3656 GN=LOC103483243 PE=4 SV=1[more]
A0A6J1EEC78.4e-14081.90WRKY transcription factor 22 OS=Cucurbita moschata OX=3662 GN=LOC111431800 PE=4 ... [more]
Match NameE-valueIdentityDescription
NP_001267594.19.4e-186100.00WRKY transcription factor 22-like [Cucumis sativus] >ADU52510.1 WRKY protein [Cu... [more]
TYK17626.19.5e-17092.63WRKY transcription factor 22 [Cucumis melo var. makuwa][more]
XP_008437986.12.8e-16992.33PREDICTED: WRKY transcription factor 22 [Cucumis melo][more]
XP_038880705.18.9e-16089.05WRKY transcription factor 22 [Benincasa hispida][more]
KAG6582635.11.6e-14081.95Proline iminopeptidase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT4G01250.16.1e-5847.29WRKY family transcription factor [more]
AT5G52830.12.6e-4038.76WRKY DNA-binding protein 27 [more]
AT2G34830.15.6e-3552.67WRKY DNA-binding protein 35 [more]
AT4G23550.15.4e-3039.27WRKY family transcription factor [more]
AT1G30650.14.6e-2936.33WRKY DNA-binding protein 14 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 158..218
e-value: 7.6E-37
score: 138.4
IPR003657WRKY domainPFAMPF03106WRKYcoord: 160..217
e-value: 4.4E-25
score: 87.7
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 153..219
score: 34.77314
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 142..212
e-value: 1.9E-31
score: 109.5
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 156..219
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..245
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 213..268
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 87..109
NoneNo IPR availablePANTHERPTHR32096:SF92TRANSCRIPTION FACTOR WRKY FAMILY-RELATEDcoord: 1..319
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 1..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G08490.1CSPI03G08490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding