CSPI03G07210 (gene) Cucumber (PI 183967) v1

BLAST of CSPI03G07210 vs. ExPASy Swiss-Prot
Match: Q0P5W1 (Vacuolar protein sorting-associated protein 8 homolog OS=Mus musculus OX=10090 GN=Vps8 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.3e-91
Identity = 346/1443 (23.98%), Postives = 614/1443 (42.55%), Query Frame = 0

Query: 448  ISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGS 507
            IS  I S + + + G P  +AV ++ IAVG S G  ++          D   A  + LGS
Sbjct: 141  ISAQIVSAADKVDAGLPTAIAV-SSLIAVGTSHGLALIF---------DQNQALRLCLGS 200

Query: 508  QGDKSTAPATS-LCFSQQGDLLLAGYSDGHITVWDVLRASAAKVISGEH--ASPVVHSLF 567
                    A S L  +     LL G++ G IT+WD+      + I+  H   + ++H  F
Sbjct: 201  TSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKF 260

Query: 568  LGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLL 627
                        A+  DS G V   TF  V  +   + +++CL  G K G V     L  
Sbjct: 261  TDDPT------LAICNDSGGSVFELTFKRV--MGVRTCESRCLFSGSK-GEVCCIEPL-- 320

Query: 628  NEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTAL 687
                     P L +  ++  S+ +M                            A+    L
Sbjct: 321  ------HSKPELKDHPITQFSLLAM----------------------------ASLTKIL 380

Query: 688  VVRLSPTVEVYAQLSKPDG-IREGSMPYTAWK-CSQSFETSPSEAVERVSLLAIAWDKMV 747
            V+ L P+++V+  ++ P G +   S+P  AW   + +   +P  A  R  ++     K  
Sbjct: 381  VIGLKPSLKVW--MTFPYGRMDPSSVPLLAWHFVAVNNSVNPMLAFCRGDMVHFLLVKRD 440

Query: 748  QVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTGQLFLFEKDGTMIHQTSIFVD 807
            +   +  T+ K      L    I   W++ + +V+L    +L + ++      Q  +   
Sbjct: 441  ESGAIHVTKQK---HLHLYYDLINFTWINSRTVVLLDSVEKLHVIDRQ----TQEELETM 500

Query: 808  GFVKEDFIAYHTHFANIL--GN--------PEKAYHNCVAVRGASIYVLGPMHLVISRLL 867
               +   +   +HF ++   GN         EKA +  ++  G  I+ LG   + +  L 
Sbjct: 501  EISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMMLR 560

Query: 868  PWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPFLIELLLSYVD 927
             W+ER+  L K      AL++A + ++G A  V+ L   +   + +V   ++E+L  Y D
Sbjct: 561  SWRERMDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDVSKRKAVVADRMVEILFHYAD 620

Query: 928  EVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCVHISRTDILFD 987
                             +K  D           +++ +     V V++C+ + R D+LF 
Sbjct: 621  RAL--------------KKCPD-----QGKIQVMEQHFQDTVPVIVDYCLLLQRKDLLFG 680

Query: 988  EIFSKF-VGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQCVLHM 1047
            +++ K       +  FLE LEPYIL D L  + P++M+ L+ H+  K  L+ VE  ++HM
Sbjct: 681  QMYDKLSENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLLENVEALIVHM 740

Query: 1048 DISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLR------TSKSKHAS 1107
            DI+SLD  QVV +C ++ LY A+VY++N+G+++F +P+E+L  V+        + +    
Sbjct: 741  DITSLDIQQVVLMCWENRLYDAMVYVYNRGMNEFISPMEKLFKVIAPPLNAGKTLTDEQV 800

Query: 1108 SLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISSKSSE 1167
             +G K LVY+  C +G A+P   G +    V  +++++ +FL+         S+ + S E
Sbjct: 801  VMGNKLLVYISCCLAGRAYP--LGDIPEDLVPLVKNQVFEFLIR------LHSVEASSEE 860

Query: 1168 VGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSISGRKNF 1227
                 +  LL  DT   L+VL   F +                    + +K ++  +   
Sbjct: 861  EVYPYVRTLLHFDTREFLNVLALTFED-------------------FKNDKQAVEYQ--- 920

Query: 1228 LIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACGKAT-- 1287
              Q +VD L+ V+   +  +D +P+              ++  LF F+A  +A    T  
Sbjct: 921  --QRIVDILLKVM---VENSDFTPS--------------QVGCLFTFLARQLAKPDNTLF 980

Query: 1288 VSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWNPSS 1347
            V++ +  Q+LE L S  D                    +R++ +L LL+      +  S 
Sbjct: 981  VNRTLFDQVLEFLCSPDD---------------DSRHSERQQVLLELLQAGGIVQFEESR 1040

Query: 1348 VLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNS--EQT 1407
            ++RM EKA+F+Q+C  ++   HQY   +D Y+ D       F +I+  L   G+S  E+ 
Sbjct: 1041 LIRMAEKAEFYQICEFMYEREHQYDKIIDCYLHDPLREEEVFNYIHNILSIPGHSAEEKQ 1100

Query: 1408 EFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHLS 1467
                  ++ + EL +L       LV  HF+  +  ++ QL+N    LF +L++L+     
Sbjct: 1101 SVWQKAMNHMEELVSLKPCKAAELVATHFSEQIEVVIGQLQNQ-LLLFKFLRSLL----- 1160

Query: 1468 GSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDTIELYVELLCQHE 1527
                      D   GV+ +        Q+L   P +++           E ++ELLCQ  
Sbjct: 1161 ----------DPREGVHVN--------QELLQIPPHIT-----------EQFIELLCQFS 1220

Query: 1528 RESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHDL 1587
             + V++ L+  + YR+E  +++ Q+Y++ +  A+LLE+ GD   A  L L  L  +  ++
Sbjct: 1221 PDQVIQTLQVLECYRLEETIQITQKYQLHEVTAYLLEKKGDAHGAFLLLLERLQSRLQEM 1280

Query: 1588 EAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQTL 1647
                           +   +N    + L+ V    V     I LCQRN+  LN ++ + L
Sbjct: 1281 ---------------TRQDENTKEDILLKGVEDTMV---ETIALCQRNSQNLNQQQREAL 1340

Query: 1648 WFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHLL 1707
            WF LL++   P                 Q L+ S+ +                    H  
Sbjct: 1341 WFPLLEAMMTP-----------------QKLSSSAAA-------------------PHPH 1342

Query: 1708 RKLFSQFIREIVEGMMGYVHLPTIMSRLLYD--NGSQEFGDFKLTILGMLGTFGFERRIL 1767
             +       +++  M  ++ LP+I+ R+L D   G  + G+ +  ILGML TF +E+ +L
Sbjct: 1401 CEALKSLTMQVLNSMAAFIALPSILQRILQDPIYGKGKLGEIQGLILGMLDTFNYEQTLL 1342

Query: 1768 DSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCIC-NRLLVKSSSSYRVRVFNCGHATH 1827
            ++  +L+  D  +++  L+   + G  P+   C IC  +   +   +  + VF+CGH  H
Sbjct: 1461 ETTASLLNQDLHWSLCNLRASVSRGLNPKQDYCSICLQQYKRRQEMADEIIVFSCGHLYH 1342

Query: 1828 ---LQCEDLENEASGGD-YTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVS 1858
               LQ ++   E  G   + C  C  SN++ G  S+ P+E    NK    T S    S S
Sbjct: 1521 SFCLQSKECTLEVEGQTRWACHKCSSSNKA-GKLSENPSE----NKKGRITSSQVKMSPS 1342

BLAST of CSPI03G07210 vs. ExPASy Swiss-Prot
Match: Q8N3P4 (Vacuolar protein sorting-associated protein 8 homolog OS=Homo sapiens OX=9606 GN=VPS8 PE=1 SV=3)

HSP 1 Score: 337.0 bits (863), Expect = 1.4e-90
Identity = 335/1414 (23.69%), Postives = 600/1414 (42.43%), Query Frame = 0

Query: 448  ISRTISSHSFRREHGFPQVLAVHANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGS 507
            IS  I S + + + G P  +AV ++ IAVG S G  ++          D   A  + LGS
Sbjct: 141  ISAQIVSAADKVDAGLPTAIAV-SSLIAVGTSHGLALIFGK-------DQNQALRLCLGS 200

Query: 508  QGDKSTAPATS-LCFSQQGDLLLAGYSDGHITVWDVLRASAAKVISGEH--ASPVVHSLF 567
                    A S L  +     LL G++ G IT+WD+      + I+  H   + ++H  F
Sbjct: 201  TSVGGQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITDAHPPGTAILHIKF 260

Query: 568  LGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLL 627
                        A+  DS G V   TF  V  +   + +++CL  G K G V     L  
Sbjct: 261  TDDPT------LAICNDSGGSVFELTFKRV--MGVRTCESRCLFSGSK-GEVCCIEPL-- 320

Query: 628  NEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTAL 687
                     P L +  ++  S+ +M                            A+    L
Sbjct: 321  ------HSKPELKDHPITQFSLLAM----------------------------ASLTKIL 380

Query: 688  VVRLSPTVEVYAQLSKPDG-IREGSMPYTAWK--CSQSFETSPSEAVERVSLLAIAWDKM 747
            V+ L P+++V+  ++ P G +   S+P  AW     Q++  +P  A  R  ++     K 
Sbjct: 381  VIGLKPSLKVW--MTFPYGRMDPSSVPLLAWHFVAVQNY-VNPMLAFCRGDVVHFLLVKR 440

Query: 748  VQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTVTGQLFLFEKDGTMIHQTSIFV 807
             +   +  T+ K      L    I   W++ + +V+L    +L + ++      Q  +  
Sbjct: 441  DESGAIHVTKQK---HLHLYYDLINFTWINSRTVVLLDSVEKLHVIDRQ----TQEELET 500

Query: 808  DGFVKEDFIAYHTHFANIL--GN--------PEKAYHNCVAVRGASIYVLGPMHLVISRL 867
                +   +   +HF ++   GN         EKA +  ++  G  I+ LG   + +  L
Sbjct: 501  VEISEVQLVYNSSHFKSLATGGNVSQALALVGEKACYQSISSYGGQIFYLGTKSVYVMML 560

Query: 868  LPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVMPFLIELLLSYV 927
              W+ERV  L K      AL++A + ++G A  V+ L       + +V   ++E+L  Y 
Sbjct: 561  RSWRERVDHLLKQDCLTEALALAWSFHEGKAKAVVGLSGDASKRKAIVADRMVEILFHYA 620

Query: 928  DEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEFCVHISRTDILF 987
            D                 +K  D           +++ +  +  V V++C+ + R D+LF
Sbjct: 621  DRAL--------------KKCPD-----QGKIQVMEQHFQDMVPVIVDYCLLLQRKDLLF 680

Query: 988  DEIFSKF-VGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQCVLH 1047
             +++ K       +  FLE LEPYIL D L  + P++M+ L+ H+  K  ++ VE  ++H
Sbjct: 681  SQMYDKLSENSVAKGVFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVH 740

Query: 1048 MDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLR------TSKSKHA 1107
            MDI+SLD  QVV +C ++ LY A++Y++N+G+++F +P+E+L  V+        + +   
Sbjct: 741  MDITSLDIQQVVLMCWENRLYDAMIYVYNRGMNEFISPMEKLFRVIAPPLNAGKTLTDEQ 800

Query: 1108 SSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSISSKSS 1167
              +G K LVY+  C +G A+P   G +    V  +++++ +FL+    A      +S   
Sbjct: 801  VVMGNKLLVYISCCLAGRAYP--LGDIPEDLVPLVKNQVFEFLIRLHSAE-----ASPEE 860

Query: 1168 EVGCLNLYP----LLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSIS 1227
            E+     YP    LL  DT   L+VL   F +                    + +K ++ 
Sbjct: 861  EI-----YPYIRTLLHFDTREFLNVLALTFED-------------------FKNDKQAVE 920

Query: 1228 GRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACG 1287
             +     Q +VD L+ V+   +  +D +P+              ++  LF F+A  +A  
Sbjct: 921  YQ-----QRIVDILLKVM---VENSDFTPS--------------QVGCLFTFLARQLAKP 980

Query: 1288 KAT--VSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETH 1347
              T  V++ +  Q+LE L S  D                    +R++ +L LL+      
Sbjct: 981  DNTLFVNRTLFDQVLEFLCSPDD---------------DSRHSERQQVLLELLQAGGIVQ 1040

Query: 1348 WNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNS 1407
            +  S ++RM EKA+F+Q+C  ++   HQY   +D Y++D       F +I+  L   G+S
Sbjct: 1041 FEESRLIRMAEKAEFYQICEFMYEREHQYDKIIDCYLRDPLREEEVFNYIHNILSIPGHS 1100

Query: 1408 --EQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLI 1467
              E+       +  I EL +L       LV  HF+  +  ++ +L+N    LF +L++L+
Sbjct: 1101 AEEKQSVWQKAMDHIEELVSLKPCKAAELVATHFSGHIETVIKKLQNQV-LLFKFLRSLL 1160

Query: 1468 EVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDTIELYVEL 1527
                           D   G+                   +++   + ++    E ++EL
Sbjct: 1161 ---------------DPREGI-------------------HVNQELLQISPCITEQFIEL 1220

Query: 1528 LCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDK 1587
            LCQ     V++ L+  + YR+E  +++ Q+Y++ +  A+LLE+ GD+  A  + L  L  
Sbjct: 1221 LCQFNPTQVIETLQVLECYRLEETIQITQKYQLHEVTAYLLEKKGDIHGAFLIMLERLQS 1280

Query: 1588 KFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSE 1647
            K  ++               ++  +N      L++V    V     I LCQRN+  LN +
Sbjct: 1281 KLQEV---------------THQGENTKEDPSLKDVEDTMV---ETIALCQRNSHNLNQQ 1314

Query: 1648 ESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNK 1707
            + + LWF LL++   P                          QK           L S+ 
Sbjct: 1341 QREALWFPLLEAMMAP--------------------------QK-----------LSSSA 1314

Query: 1708 VAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYD--NGSQEFGDFKLTILGMLGTFGF 1767
            + HL  +       +++  M  ++ LP+I+ R+L D   G  + G+ +  ILGML TF +
Sbjct: 1401 IPHLHSEALKSLTMQVLNSMAAFIALPSILQRILQDPVYGKGKLGEIQGLILGMLDTFNY 1314

Query: 1768 ERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCIC-NRLLVKSSSSYRVRVFNC 1824
            E+ +L++  +L+  D  +++  L+     G  P+   C IC  +   +   +  + VF+C
Sbjct: 1461 EQTLLETTTSLLNQDLHWSLCNLRASVTRGLNPKQDYCSICLQQYKRRQEMADEIIVFSC 1314

BLAST of CSPI03G07210 vs. ExPASy Swiss-Prot
Match: Q9VRX2 (Vacuolar protein sorting-associated protein 8 homolog OS=Drosophila melanogaster OX=7227 GN=Vps8 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 4.7e-30
Identity = 147/615 (23.90%), Postives = 257/615 (41.79%), Query Frame = 0

Query: 826  AYHNCVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGV 885
            A +N V  RG  +YVLG   L I  +  W ER+  L K   W  A  +A+   DG+   V
Sbjct: 435  ACYNSVVSRGTQLYVLGARSLHIIGVRTWSERISFLVKHHRWQEACQLAL---DGYIASV 494

Query: 886  IDLPRSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSE 945
             D PR     +E ++                                  M  + + A+S 
Sbjct: 495  -DRPRKRAQAKERII----------------------------------MLFKEYIANSA 554

Query: 946  IKEQYNRVGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPP 1005
               +Y    G  V   + +   D+L+ +++ K       + FL+ +  +I K+ + S+ P
Sbjct: 555  RAPEY--CLGAIVNCLITVGELDLLWTQLWEKLHN-SSTELFLQHISEHIEKETIHSVNP 614

Query: 1006 EIMQALVEHYSHKGWLQ----RVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKG 1065
             I QALV++     WL+    ++EQ +L +D   LD NQV++  + H L+ A +YL  + 
Sbjct: 615  VISQALVDY-----WLEHSPAKLEQLILKLDWMCLDLNQVLKAVKKHRLFRAQIYLNTQA 674

Query: 1066 LDDFRTPLEELLAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRD 1125
            L+D+   L ELL ++   +    + LG   LVY+  C +G  +P G+  +    V  ++ 
Sbjct: 675  LNDYTAALTELLPLVTPDE----TDLGNCLLVYVSSCLAGREYPSGE--IPVELVHQVKH 734

Query: 1126 ELLQFLLENSDAVDTRSISSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISS 1185
            ++L+ L          S  +   E+    L  LL+ DT  TL+V+  AF E E    +  
Sbjct: 735  DVLRCL------TSQHSKENAGDELPYPYLRALLKFDTRETLNVISLAFQEREFSNEL-- 794

Query: 1186 LDGPVDTSMQLQEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWP 1245
                             IS RK      +++ L+ ++         SP  +N T    W 
Sbjct: 795  ----------------GISHRK-----RIINLLLEIM---------SP--ENAT----W- 854

Query: 1246 SKKELIHLFDFIATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTA-NSVLS 1305
               E+  L +FIA  ++       + ++ ++L HL       E+      R  A + +LS
Sbjct: 855  --AEIGCLLNFIAQQISMQCLPRDRQLLERVLSHLAQEEIANESSRQHSERENAWHELLS 914

Query: 1306 RKREKQVLSLLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDE 1365
                 ++ S               LR+ EKA+ + V   +     +Y + LDSY+++   
Sbjct: 915  SNCLAEISS-----------DEEQLRLAEKAKCYCVVEYLLEKLERYDTILDSYIRNEAR 935

Query: 1366 PIHTFTFINRTLLELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQ 1425
                F ++ R    + + +++ FR +    + EL  +N   T  L+  H+   ++ +L  
Sbjct: 975  HETMFAYMER---HVASPKRSIFRQLK-RNLRELLTINAKETTRLLSLHYPEKINELLDN 935

Query: 1426 LRNHPRSLFLYLKTL 1436
            LR     L+L+LK L
Sbjct: 1035 LRREENLLYLFLKCL 935

BLAST of CSPI03G07210 vs. ExPASy Swiss-Prot
Match: P39702 (Vacuolar protein sorting-associated protein 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VPS8 PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-09
Identity = 31/157 (19.75%), Postives = 83/157 (52.87%), Query Frame = 0

Query: 977  KFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGWLQRVEQCVLHMDISSL 1036
            +F  ++    + E++   + +  + S+ P + ++++++Y+ +  L+ +E  ++ ++ ++L
Sbjct: 529  EFFELKDNAVYFEVVANIVAQGSVTSISPVLFRSIIDYYAKEENLKVIEDLIIMLNPTTL 588

Query: 1037 DFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSKSK----HASSLGYKTL 1096
            D +  V+LC+ + L+  L+Y++NK  DD++TP+ +L+  +     K    +   +  +T 
Sbjct: 589  DVDLAVKLCQKYNLFDLLIYIWNKIFDDYQTPVVDLIYRISNQSEKCVIFNGPQVPPETT 648

Query: 1097 V--YLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFL 1128
            +  Y+ Y  +G  +P         +   ++ EL  F+
Sbjct: 649  IFDYVTYILTGRQYPQNLSISPSDKCSKIQRELSAFI 685

BLAST of CSPI03G07210 vs. ExPASy TrEMBL
Match: A0A0A0L2X7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G116870 PE=3 SV=1)

HSP 1 Score: 3823.1 bits (9913), Expect = 0.0e+00
Identity = 1955/1963 (99.59%), Postives = 1956/1963 (99.64%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSS-GDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60
            MTEELTDTETLPPMELDLNAFIHAHLSS GDDDDDDDLSFPHRSIDEILNDSSSSTSPSP
Sbjct: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60

Query: 61   SSSPHFPPPRDRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTVSHSKVG 120
            SSSPHFPPPR RRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGT SHSKVG
Sbjct: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVG 120

Query: 121  ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV 180
            ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV
Sbjct: 121  ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV 180

Query: 181  LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN 240
            LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN
Sbjct: 181  LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN 240

Query: 241  NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVKNV 300
            NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAE   KNV
Sbjct: 241  NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAE---KNV 300

Query: 301  CSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLA 360
            CSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLA
Sbjct: 301  CSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLA 360

Query: 361  IEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEG 420
            IEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEG
Sbjct: 361  IEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEG 420

Query: 421  VAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMS 480
            VAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMS
Sbjct: 421  VAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMS 480

Query: 481  KGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITV 540
            KGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITV
Sbjct: 481  KGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITV 540

Query: 541  WDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNR 600
            WDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNR
Sbjct: 541  WDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNR 600

Query: 601  FSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSG 660
            FSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSG
Sbjct: 601  FSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSG 660

Query: 661  WKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQS 720
            WKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQS
Sbjct: 661  WKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQS 720

Query: 721  FETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILT 780
            FETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILT
Sbjct: 721  FETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILT 780

Query: 781  VTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIY 840
            VTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIY
Sbjct: 781  VTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIY 840

Query: 841  VLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELV 900
            VLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELV
Sbjct: 841  VLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELV 900

Query: 901  MPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVE 960
            MPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVE
Sbjct: 901  MPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVE 960

Query: 961  FCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKG 1020
            FCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKG
Sbjct: 961  FCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKG 1020

Query: 1021 WLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTS 1080
            WLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTS
Sbjct: 1021 WLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTS 1080

Query: 1081 KSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSI 1140
            KSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSI
Sbjct: 1081 KSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSI 1140

Query: 1141 SSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSI 1200
            S+KSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSI
Sbjct: 1141 SNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSI 1200

Query: 1201 SGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVAC 1260
            SGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVAC
Sbjct: 1201 SGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVAC 1260

Query: 1261 GKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHW 1320
            GKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHW
Sbjct: 1261 GKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHW 1320

Query: 1321 NPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSE 1380
            NPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSE
Sbjct: 1321 NPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSE 1380

Query: 1381 QTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVH 1440
            QTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVH
Sbjct: 1381 QTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVH 1440

Query: 1441 LSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDTIELYVELLCQ 1500
            LSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDD IELYVELLCQ
Sbjct: 1441 LSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQ 1500

Query: 1501 HERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFH 1560
            HERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFH
Sbjct: 1501 HERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFH 1560

Query: 1561 DLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQ 1620
            DLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQ
Sbjct: 1561 DLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQ 1620

Query: 1621 TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAH 1680
            TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAH
Sbjct: 1621 TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAH 1680

Query: 1681 LLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRIL 1740
            LLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRIL
Sbjct: 1681 LLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRIL 1740

Query: 1741 DSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHL 1800
            DSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHL
Sbjct: 1741 DSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHL 1800

Query: 1801 QCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQET 1860
            QCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQET
Sbjct: 1801 QCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQET 1860

Query: 1861 DLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESS 1920
            DLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESS
Sbjct: 1861 DLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESS 1920

Query: 1921 GGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            GGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS
Sbjct: 1921 GGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1960

BLAST of CSPI03G07210 vs. ExPASy TrEMBL
Match: A0A1S3AUY3 (vacuolar protein sorting-associated protein 8 homolog OS=Cucumis melo OX=3656 GN=LOC103483112 PE=3 SV=1)

HSP 1 Score: 3603.1 bits (9342), Expect = 0.0e+00
Identity = 1854/1970 (94.11%), Postives = 1886/1970 (95.74%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDLSFPHRSIDEILNDSSSSTSPSPS 60
            MTEELTDT TLPPMELDLNAFIHAHLSSGDDDDDDDLSFPHRSIDEILNDSSSSTS SPS
Sbjct: 1    MTEELTDTRTLPPMELDLNAFIHAHLSSGDDDDDDDLSFPHRSIDEILNDSSSSTSSSPS 60

Query: 61   SSPHFPPPRDRRNIVAGDDGVSASPSTSPY--------KDSEAARNNPWNEKSAQLKPGT 120
            SSPH PP R RRNIVAG+ GVSASPSTSP+        KDSEA RNNPWNEKSAQ KPG 
Sbjct: 61   SSPHSPPSRGRRNIVAGNGGVSASPSTSPFKSLLEETIKDSEAPRNNPWNEKSAQSKPGK 120

Query: 121  VSHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRR 180
            VSHSK+GELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRR
Sbjct: 121  VSHSKIGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRR 180

Query: 181  AGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVS 240
            AGYGNM LDDDELASSSAVDSEF SDSLYH NIH KESGENSISVVDRITDYQ+AS +VS
Sbjct: 181  AGYGNMALDDDELASSSAVDSEFLSDSLYHTNIHLKESGENSISVVDRITDYQVASRDVS 240

Query: 241  GELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPA 300
             ELW  NNIRD VPHNDEFRMTEDMEFEAE SSVDDVNF ESL+TVPP ETNDRSLLGPA
Sbjct: 241  -ELWDRNNIRDSVPHNDEFRMTEDMEFEAEPSSVDDVNFNESLTTVPPAETNDRSLLGPA 300

Query: 301  EKNVKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSK 360
            E   KNVCSTDAHPTELDVDESNEGAIPR TEPDDE SAVGYGS ELETQDFEKYHQPSK
Sbjct: 301  E---KNVCSTDAHPTELDVDESNEGAIPRSTEPDDEGSAVGYGSPELETQDFEKYHQPSK 360

Query: 361  DTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFT 420
            DTEVDLAIEDPSIVNDIIESGETTEQ DNLQIGK PE + VSSTNPL+LAEEIEKKQAFT
Sbjct: 361  DTEVDLAIEDPSIVNDIIESGETTEQLDNLQIGKHPETMPVSSTNPLELAEEIEKKQAFT 420

Query: 421  ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN 480
            ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN
Sbjct: 421  ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN 480

Query: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGY 540
            YIAVGMSKG+IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAP TSLCFSQQ DLLLAGY
Sbjct: 481  YIAVGMSKGSIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPVTSLCFSQQADLLLAGY 540

Query: 541  SDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
            SDGHITVWDVLRASAAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 541  SDGHITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600

Query: 601  VVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG 660
            VVPLLNRFS KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG
Sbjct: 601  VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG 660

Query: 661  VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT 720
            VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSP+VEVYAQLSKPDGIREGSMPYT
Sbjct: 661  VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPSVEVYAQLSKPDGIREGSMPYT 720

Query: 721  AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDD 780
            AWKCSQSFETS SEAVERVSLLAIAWDKMVQVAKLVKTELKVCG WSLESAAIGVVWLDD
Sbjct: 721  AWKCSQSFETSSSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGNWSLESAAIGVVWLDD 780

Query: 781  QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVA 840
            QVLVILTVTGQLFLFEKDGTMIHQTS+F DGFVKEDFIAYHTHFAN+LG+PEKAYHNCVA
Sbjct: 781  QVLVILTVTGQLFLFEKDGTMIHQTSVFADGFVKEDFIAYHTHFANVLGHPEKAYHNCVA 840

Query: 841  VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 900
            VRGASIYVLGP HLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL
Sbjct: 841  VRGASIYVLGPTHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 900

Query: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNR 960
            ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+T E  SAHSEIKEQYNR
Sbjct: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSERDSAHSEIKEQYNR 960

Query: 961  VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
            VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 961  VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020

Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
            EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080

Query: 1081 LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
            LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD
Sbjct: 1081 LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140

Query: 1141 AVDTRSISSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQL 1200
            AVDTRSIS+KSSEVGCLNLY LLELDTEATLDVLRCAFVE E LK  SSLDGPVD  M+L
Sbjct: 1141 AVDTRSISNKSSEVGCLNLYHLLELDTEATLDVLRCAFVEVEFLKTNSSLDGPVDAIMEL 1200

Query: 1201 QEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDF 1260
            Q+EKNSISGRKNFLIQNVVDALVHVL KAICETDESP GDNITLVDDWPSKKELIHLFDF
Sbjct: 1201 QDEKNSISGRKNFLIQNVVDALVHVLGKAICETDESPDGDNITLVDDWPSKKELIHLFDF 1260

Query: 1261 IATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLE 1320
            IATYVACGKATVSKDVVGQILEHLISN+ IPET SDFLPRVTANSV SRKREKQVLSLLE
Sbjct: 1261 IATYVACGKATVSKDVVGQILEHLISNTHIPET-SDFLPRVTANSVHSRKREKQVLSLLE 1320

Query: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTL 1380
            V+PETHWNPSSVLRMCEKAQFFQVCGLIHSI  QYSSALDSYMKDV EPIH F FINR L
Sbjct: 1321 VVPETHWNPSSVLRMCEKAQFFQVCGLIHSIGCQYSSALDSYMKDVGEPIHAFAFINRAL 1380

Query: 1381 LELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYL 1440
            L+L NSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFN+DVSNIL QLRNHPRSLFLYL
Sbjct: 1381 LKLSNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNDDVSNILLQLRNHPRSLFLYL 1440

Query: 1441 KTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDTIEL 1500
            KTLIEVHLSGS DFSCLKKDDNLGVNYSTKG+DDYL+KLSDFPKYLSNNPVDVTDD IEL
Sbjct: 1441 KTLIEVHLSGSLDFSCLKKDDNLGVNYSTKGLDDYLKKLSDFPKYLSNNPVDVTDDIIEL 1500

Query: 1501 YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
            YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1501 YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560

Query: 1561 SLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPR 1620
            SLDKKFHDLEAAVGA VSN ASSGS+DSQ+F+SVLKLQEVN V+VLLHACIGLCQRNTPR
Sbjct: 1561 SLDKKFHDLEAAVGAIVSNGASSGSSDSQHFDSVLKLQEVNTVEVLLHACIGLCQRNTPR 1620

Query: 1621 LNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRIL 1680
            LN EES+TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNE S SQKDKEANIVTWRIL
Sbjct: 1621 LNCEESETLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNEPSSSQKDKEANIVTWRIL 1680

Query: 1681 KSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1740
            KSNK AH+LRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF
Sbjct: 1681 KSNKAAHILRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1740

Query: 1741 GFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN 1800
            GFERRILD+AKALIEDDSFYTM+LLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN
Sbjct: 1741 GFERRILDTAKALIEDDSFYTMNLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN 1800

Query: 1801 CGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGAS 1860
            CGHATHLQCEDLENEASGGD TCPICVHSNQSQGSKSKAPTEYSLVNKFSSRT SSSGAS
Sbjct: 1801 CGHATHLQCEDLENEASGGDSTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTSSSSGAS 1860

Query: 1861 VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH 1920
            VSYPQETD+LELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH
Sbjct: 1861 VSYPQETDILELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH 1920

Query: 1921 LLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            LLVGESS GREKVEKLNKSRQLT VKVKRPSSLRFPLK SLFGKEKMTNS
Sbjct: 1921 LLVGESSSGREKVEKLNKSRQLTEVKVKRPSSLRFPLKASLFGKEKMTNS 1965

BLAST of CSPI03G07210 vs. ExPASy TrEMBL
Match: A0A6J1EC21 (vacuolar protein sorting-associated protein 8 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431840 PE=3 SV=1)

HSP 1 Score: 3288.4 bits (8525), Expect = 0.0e+00
Identity = 1702/1973 (86.26%), Postives = 1799/1973 (91.18%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGDDDDD---DDLSFPHRSIDEILNDSSSSTSP 60
            MT+ELTDTETLPPMELDLNAFIHAHLSSGDDDDD   DDLSFPHRSIDEILN+SSSSTS 
Sbjct: 1    MTKELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDEDDLSFPHRSIDEILNESSSSTSS 60

Query: 61   SPSSSPHFPPPRDRRNIVAGDDGVSASPSTSPYKD--------SEAARNNPWNEKSAQLK 120
            SPSS P+ PPPRD R+I A D   SAS S  P+K         S+  R+N  NEKS QLK
Sbjct: 61   SPSSPPNSPPPRDHRSIAARDGRASASRSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLK 120

Query: 121  PGTVSHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIK 180
            PG+VSH+KVGELTDDPFRRGSR LPSLFG VRSNAKPGAALAAAAAASRS PAPHAAAIK
Sbjct: 121  PGSVSHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180

Query: 181  SRRAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASM 240
            SRR+G+G++VLDDDELASSSAVDSEF  D LY    HSKES E SIS+V+R  DYQ AS+
Sbjct: 181  SRRSGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGASV 240

Query: 241  NVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLL 300
            NV GE WA +NIRD V +NDEFR+T+D E EAE S VDDVNF ES +T+PPVE N RSL 
Sbjct: 241  NVGGEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFDESSTTLPPVEANGRSLS 300

Query: 301  GPAEKNVKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQ 360
              A+    NVCS DA PT LDVDESNEGA P   +PD + SAVGYG LELETQDFEK  Q
Sbjct: 301  DSAD---NNVCSMDAEPTVLDVDESNEGAFPSSPKPDYDRSAVGYGRLELETQDFEKRFQ 360

Query: 361  PSKDTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQ 420
            PSKD+EV LAIED S+VNDI ES ET EQ DN   G+R E +S+SS+NPL+LAEEIEKKQ
Sbjct: 361  PSKDSEV-LAIEDLSVVNDISESRETAEQLDNFHTGERAETMSLSSSNPLELAEEIEKKQ 420

Query: 421  AFTALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAV 480
            AFTALHWEEGVAAQPMRLEGIKG TT LGYFDIQADNSISRTISSHSF+REHGFPQ LAV
Sbjct: 421  AFTALHWEEGVAAQPMRLEGIKGGTTALGYFDIQADNSISRTISSHSFKREHGFPQALAV 480

Query: 481  HANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLL 540
            HANYIAVGMSKGNIVVVASKYSAQNGDNMDAKM+LLGSQGDKSTAP TSLCF+QQGDLLL
Sbjct: 481  HANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGSQGDKSTAPVTSLCFNQQGDLLL 540

Query: 541  AGYSDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH 600
            AGYSDG +TVWDVLRA+AAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH
Sbjct: 541  AGYSDGQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH 600

Query: 601  TFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSM 660
            TFSVVPLLNRFS KTQCLLDGQKTGTVLSASALLLNEF GSSLPP+LSNVAVSTSSIGSM
Sbjct: 601  TFSVVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSM 660

Query: 661  MGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSM 720
            MGGVVGGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPTVEVYA+LSKPDGI+EGSM
Sbjct: 661  MGGVVGGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSM 720

Query: 721  PYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVW 780
            PYTAWKCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGV W
Sbjct: 721  PYTAWKCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAW 780

Query: 781  LDDQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHN 840
            LDDQVLVILTVTGQLFLFEKDGTMIHQTS+FVDGF KEDFIA+HTHF N+LGNPEKAYHN
Sbjct: 781  LDDQVLVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHN 840

Query: 841  CVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLP 900
            CVAVRGAS+YVLGP HLVISRLLPWKERVQVLRKAGDWM+ALSMAITIYDGHAHGVIDLP
Sbjct: 841  CVAVRGASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLP 900

Query: 901  RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQ 960
            RSLESLQELVMPFLIELLLSYVDEVFSYIS+AFCNQIEKNEKLDD+T  SHSAHSEIKEQ
Sbjct: 901  RSLESLQELVMPFLIELLLSYVDEVFSYISLAFCNQIEKNEKLDDVTSGSHSAHSEIKEQ 960

Query: 961  YNRVGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQ 1020
            YNRVGGVAVEFCVHI+RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQ
Sbjct: 961  YNRVGGVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQ 1020

Query: 1021 ALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL 1080
            ALVEHYS KGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL
Sbjct: 1021 ALVEHYSQKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL 1080

Query: 1081 EELLAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE 1140
            EELLAVL+TSKSK AS++GYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE
Sbjct: 1081 EELLAVLQTSKSKRASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE 1140

Query: 1141 NSDAVDTRSISSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTS 1200
            NSD VDTRSIS+KSSEVG LNLY LLELDT ATLDVLRCAFVEGEI+KA SSLDG VD S
Sbjct: 1141 NSDTVDTRSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEGEIVKADSSLDGSVDAS 1200

Query: 1201 MQLQEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHL 1260
            MQ+Q+E       KNFL+QNVVDAL+H+LDKAI +T  SP GDNITLV+DWPSKK+L HL
Sbjct: 1201 MQVQKE-------KNFLVQNVVDALIHILDKAISQTYGSPGGDNITLVEDWPSKKDLFHL 1260

Query: 1261 FDFIATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLS 1320
            FDF+A YVACGKATVSKDVVGQILEHLISNSDIPE   DF+  VTANSV SRKREKQVLS
Sbjct: 1261 FDFVANYVACGKATVSKDVVGQILEHLISNSDIPEMEIDFVHSVTANSVHSRKREKQVLS 1320

Query: 1321 LLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFIN 1380
            LLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSI+HQYSSALD YMKDV+EPIH F FIN
Sbjct: 1321 LLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVEEPIHAFAFIN 1380

Query: 1381 RTLLELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLF 1440
            RTL+EL  SEQTEFR VVISRIPEL NLNR  TFFLVIDHF NDVS+ILSQL NHPRSLF
Sbjct: 1381 RTLMELSYSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFGNDVSDILSQLHNHPRSLF 1440

Query: 1441 LYLKTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDT 1500
            LYLKTLIEVH SG+ +FSCLKKDDN GVNYSTKG+DDYLQKLSDFPK+LSNNPVDVTDD 
Sbjct: 1441 LYLKTLIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDI 1500

Query: 1501 IELYVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL 1560
            IELYVELLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL
Sbjct: 1501 IELYVELLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL 1560

Query: 1561 TLSSLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRN 1620
            TLSSLDKKFHDLEAAVG  V+N ASSGSNDSQ F+SVLKLQEVN + VLLHACIGLCQRN
Sbjct: 1561 TLSSLDKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRN 1620

Query: 1621 TPRLNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTW 1680
            TPRLNSEES+TLWFKLLDSFCEPLI+S+N+RTASF +NQVQFLNESS SQKDKEA+IVTW
Sbjct: 1621 TPRLNSEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTW 1680

Query: 1681 RILKSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGML 1740
            RILKSN+ AH+LR LFS+FIREIVEGM+GYVHLPTIMSRLL DNGSQEFGDFKLTILGML
Sbjct: 1681 RILKSNQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGML 1740

Query: 1741 GTFGFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVR 1800
            GTFGFERRILD+AKALIEDD+FYTMSLLKKGA+HGYAPR  VCCICNRLLVKSSSSYRVR
Sbjct: 1741 GTFGFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVR 1800

Query: 1801 VFNCGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSS 1860
            VFNCGHATHLQCE L+NEASGGD++CP+CVHSN SQ S+ KA TEYSLVNKFSSRTQSSS
Sbjct: 1801 VFNCGHATHLQCEVLDNEASGGDFSCPVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSS 1860

Query: 1861 GASVSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTK 1920
            GA VSYPQETDLLELPYTLQQIPRFEIL NLQKNQRVIDIEN+PQLRLAPPAVYHDKVTK
Sbjct: 1861 GAFVSYPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTK 1920

Query: 1921 GYHLLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            GYHLLVG+SSGG EKVEKLNKSRQL  VKVKRPSSLRFPLK +LFGKEK T S
Sbjct: 1921 GYHLLVGDSSGGIEKVEKLNKSRQLKEVKVKRPSSLRFPLKANLFGKEKTTKS 1961

BLAST of CSPI03G07210 vs. ExPASy TrEMBL
Match: A0A6J1IVQ0 (vacuolar protein sorting-associated protein 8 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479042 PE=3 SV=1)

HSP 1 Score: 3281.1 bits (8506), Expect = 0.0e+00
Identity = 1694/1970 (85.99%), Postives = 1796/1970 (91.17%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDLSFPHRSIDEILNDSSSSTSPSPS 60
            MT++LTDTETLPPMELDLNAFIHAHLSSGDDDD+DDLSFPHRSIDEILN+SSSSTS SPS
Sbjct: 1    MTKQLTDTETLPPMELDLNAFIHAHLSSGDDDDEDDLSFPHRSIDEILNESSSSTSSSPS 60

Query: 61   SSPHFPPPRDRRNIVAGDDGVSASPSTSPYKD--------SEAARNNPWNEKSAQLKPGT 120
            S P+ PPPR RR+I A D   SAS S  P+K         S+  R+N  NEKS QLKPG+
Sbjct: 61   SPPNLPPPRARRSIAARDGRASASHSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLKPGS 120

Query: 121  VSHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRR 180
            VSH+KVGELTDDPFRRGSR LPSLFG VRSNAKPGAALAAAAAASRS PAPHAAAIKSRR
Sbjct: 121  VSHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIKSRR 180

Query: 181  AGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVS 240
            +G+G++VLDDDELASSSAVDSEF  D LY    HSKES E SIS+V+R  DYQ AS    
Sbjct: 181  SGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGAS---G 240

Query: 241  GELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPA 300
            GE WA +NIRD V +NDEFR+T+D E EAE S VDDVNF ES +T+PPVE N RSL   A
Sbjct: 241  GEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFNESSTTLPPVEANGRSLSDSA 300

Query: 301  EKNVKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSK 360
            +    NVCS DA PT LDVDESNEGA     +PD ++SAVGYG LELETQDFEK  QPSK
Sbjct: 301  D---NNVCSMDAEPTVLDVDESNEGAFSCSPKPDYDKSAVGYGRLELETQDFEKRFQPSK 360

Query: 361  DTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFT 420
            D+EV LAIED S+VNDI ES ET EQ DN   G+R E +S+SSTNPL+LAEEIEKKQAFT
Sbjct: 361  DSEV-LAIEDLSVVNDISESRETAEQLDNFDTGERAETMSLSSTNPLELAEEIEKKQAFT 420

Query: 421  ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN 480
            ALHWEEGVAAQPMRLEGIKG TT LGYFDIQADN ISRTISSHSF+REHGFPQVLAVH N
Sbjct: 421  ALHWEEGVAAQPMRLEGIKGGTTALGYFDIQADNCISRTISSHSFKREHGFPQVLAVHTN 480

Query: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGY 540
            YIAVGMSKGNIVVVASKYSAQNGDNMD KM+LLGSQGDKSTAP TSLCF+QQGDLLLAGY
Sbjct: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDGKMLLLGSQGDKSTAPVTSLCFNQQGDLLLAGY 540

Query: 541  SDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
            SDG +TVWDVLRA+AAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 541  SDGQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600

Query: 601  VVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG 660
            VVPLLNRFS KTQCLLDGQKTGTVLSASALLLNEF GSSLPP+LSNVAVSTSSIGSMMGG
Sbjct: 601  VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSMMGG 660

Query: 661  VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT 720
            VVGGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPTVEVYA+LSKPDGI+EGSMPYT
Sbjct: 661  VVGGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSMPYT 720

Query: 721  AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDD 780
            AWKCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGV WLDD
Sbjct: 721  AWKCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAWLDD 780

Query: 781  QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVA 840
            QVLVILTVTGQLFLFEKDGTMIHQTS+FVDGF KEDFIA+HTHF N+LGNPEKAYHNCVA
Sbjct: 781  QVLVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHNCVA 840

Query: 841  VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 900
            VRGAS+YVLGP HLVISRLLPWKERVQVLRKAGDWM+ALSMAITIYDGHAHGVIDLPRSL
Sbjct: 841  VRGASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLPRSL 900

Query: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNR 960
            ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+T  SHS HSEIKEQYNR
Sbjct: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSGSHSEHSEIKEQYNR 960

Query: 961  VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
            VGGVAVEFCVHI+RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 961  VGGVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020

Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
            EHYS +GWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1021 EHYSQRGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080

Query: 1081 LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
            LAVL+TSKSK AS++GYK LVYLKYCFSGLAFPPGQGTL+HSRVQSLRDELLQFLLENSD
Sbjct: 1081 LAVLQTSKSKRASAIGYKILVYLKYCFSGLAFPPGQGTLSHSRVQSLRDELLQFLLENSD 1140

Query: 1141 AVDTRSISSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQL 1200
             VDTRSIS+KSSEVG LNLY LL+LDT ATLDVLRCAFVEGEI+KA SSLDG VD S+Q 
Sbjct: 1141 TVDTRSISNKSSEVGYLNLYHLLKLDTGATLDVLRCAFVEGEIVKADSSLDGSVDASIQA 1200

Query: 1201 QEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDF 1260
            Q+EKNS SGRKNFL+QNVVDALVH+LDKAI +T  SP GDNITLV+DWPSKK L HLFDF
Sbjct: 1201 QKEKNSTSGRKNFLVQNVVDALVHILDKAISQTYRSPGGDNITLVEDWPSKKNLFHLFDF 1260

Query: 1261 IATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLE 1320
            +A YV CGKAT SKDVVGQILEHLISNSDIPE   DF+  VTANSV SRKRE+QVLSLLE
Sbjct: 1261 VANYVVCGKATASKDVVGQILEHLISNSDIPEMEIDFVHSVTANSVHSRKRERQVLSLLE 1320

Query: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTL 1380
            VIPETHWNPSSVLRMCEKAQFFQVCGLIHSI+HQYSSALD YMKDVDEPIH F FINRTL
Sbjct: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVDEPIHAFAFINRTL 1380

Query: 1381 LELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYL 1440
            +EL NSEQTEFR VVISRIPEL NLNR  TFFLVIDHF+NDVS+ILSQL NHPRSLFLYL
Sbjct: 1381 MELNNSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFSNDVSDILSQLHNHPRSLFLYL 1440

Query: 1441 KTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDTIEL 1500
            KTLIEVH SG+ +FSCLKKDDN GVNYSTKG+DDYLQKLSDFPK+LSNNPVDVTDD IEL
Sbjct: 1441 KTLIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDIIEL 1500

Query: 1501 YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
            YVELLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1501 YVELLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560

Query: 1561 SLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPR 1620
            SLDKKFHDLEAAVG  V+N ASSGSNDSQ F+SVLKLQEVN + VLLHACIGLCQRNTPR
Sbjct: 1561 SLDKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRNTPR 1620

Query: 1621 LNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRIL 1680
            LNSEES+TLWFKLLDSFCEPLI+S+N+RTASF +NQVQFLNESS SQKDKEA+IVTWRIL
Sbjct: 1621 LNSEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTWRIL 1680

Query: 1681 KSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1740
            KSN+ AH+LR LFS+FIREIVEGM+GYVHLPTIMSRLL DNGSQEFGDFKLTILGMLGTF
Sbjct: 1681 KSNQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGMLGTF 1740

Query: 1741 GFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN 1800
            GFERRILD+AKALIEDD+FYTMSLLKKGA+HGYAPR  VCCICNRLLVKSSSSYRVRVFN
Sbjct: 1741 GFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVRVFN 1800

Query: 1801 CGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGAS 1860
            CGHATHLQCE L+NEASGGD++C +CVHSN SQ S+ KA TEYSLVNKFSSRTQSSSGAS
Sbjct: 1801 CGHATHLQCEVLDNEASGGDFSCQVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSSGAS 1860

Query: 1861 VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH 1920
            VSYPQETDLLELPYTLQQIPRFEIL NLQKNQRVIDIEN+PQLRLAPPAVYHDKVTKG+H
Sbjct: 1861 VSYPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTKGFH 1920

Query: 1921 LLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            LLVG+SSGG EKVEKLNKSRQL  VKVK+PSSLRFPLK +LFGKEK + S
Sbjct: 1921 LLVGDSSGGVEKVEKLNKSRQLKEVKVKKPSSLRFPLKANLFGKEKTSKS 1962

BLAST of CSPI03G07210 vs. ExPASy TrEMBL
Match: A0A6J1CRK3 (vacuolar protein sorting-associated protein 8 homolog OS=Momordica charantia OX=3673 GN=LOC111013938 PE=3 SV=1)

HSP 1 Score: 2975.3 bits (7712), Expect = 0.0e+00
Identity = 1545/1962 (78.75%), Postives = 1663/1962 (84.76%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDLSFPHRSIDEILNDSSSSTSPSPS 60
            MT+ELTDT  LP MELDLNAFIHAHLSSGDDDDDDD SFPHR+IDEIL +S S     PS
Sbjct: 1    MTKELTDTGVLPLMELDLNAFIHAHLSSGDDDDDDDPSFPHRTIDEILKESGS----PPS 60

Query: 61   SSPHFPPPRDRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTVSHSKVGE 120
            S PH PP         GD  V+     S                                
Sbjct: 61   SPPHSPP-------YGGDRAVATRDGNS-------------------------------- 120

Query: 121  LTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMVL 180
                                                                      V+
Sbjct: 121  ---------------------------------------------------------TVM 180

Query: 181  DDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATNN 240
            DD +                                      DYQ+AS+NV   +WA + 
Sbjct: 181  DDTD-------------------------------------ADYQVASINVGDGVWARDR 240

Query: 241  IRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVKNVC 300
            IRD VP NDEFR+T+ ++  AE  SVDDVNF ES + +PPVE ND S+   AE   KNVC
Sbjct: 241  IRDDVPDNDEFRITKGLKCVAEQRSVDDVNFDESTTILPPVEANDGSVSDYAE---KNVC 300

Query: 301  STDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLAI 360
            ST+  P+ LDVDESNEGAIP  +EPD++ SAV YGSLE ETQDF+K  Q SKDTEVDL I
Sbjct: 301  STELRPSILDVDESNEGAIPSSSEPDNDGSAVDYGSLEWETQDFKKCLQQSKDTEVDLVI 360

Query: 361  EDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEGV 420
            +DPS+VNDI E GETTE  D+  I ++ EM+ + STNPL+ AEEIEKKQAFTALHWEEGV
Sbjct: 361  KDPSVVNDISELGETTEHMDDSTISEQAEMMLLPSTNPLEAAEEIEKKQAFTALHWEEGV 420

Query: 421  AAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMSK 480
            AAQPM+LEGIKG +TTLGYFDIQADNSISRTISSHSF+REHGFPQVLAVHANYIAVGMSK
Sbjct: 421  AAQPMKLEGIKGGSTTLGYFDIQADNSISRTISSHSFKREHGFPQVLAVHANYIAVGMSK 480

Query: 481  GNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITVW 540
            GNIVVVASKYSAQNGDNMDAKM+LLG QGDKSTAP TSLCF+ QGDLLLAGY+DGHITVW
Sbjct: 481  GNIVVVASKYSAQNGDNMDAKMLLLGLQGDKSTAPVTSLCFNLQGDLLLAGYNDGHITVW 540

Query: 541  DVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRF 600
            DVLRASAAK+ISGEH SPVVHSLFLGQ+AQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRF
Sbjct: 541  DVLRASAAKIISGEHTSPVVHSLFLGQDAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNRF 600

Query: 601  SSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSGW 660
            S KTQCLLDGQKTGTVLSASALLLNE  GSSLPPTL+NVAVSTSSIGSMMGGVVGGDSGW
Sbjct: 601  SIKTQCLLDGQKTGTVLSASALLLNESCGSSLPPTLANVAVSTSSIGSMMGGVVGGDSGW 660

Query: 661  KLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQSF 720
            KLFNEGSSLVEEGVVIFATHQTALVVRLSPT+EVYAQLSKPDG+REGSMPYTAWKCSQSF
Sbjct: 661  KLFNEGSSLVEEGVVIFATHQTALVVRLSPTLEVYAQLSKPDGVREGSMPYTAWKCSQSF 720

Query: 721  ETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILTV 780
            ETSPSEAVERVSLLAIAWDKMVQVAKLVK ELKVCGKWSLESAAIGV WLDDQVLV+LT+
Sbjct: 721  ETSPSEAVERVSLLAIAWDKMVQVAKLVKAELKVCGKWSLESAAIGVAWLDDQVLVVLTI 780

Query: 781  TGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIYV 840
            TGQLFLFEKDGTMIHQTS+FVDGF KEDFIAYHTHF N+LGNPEKAYHNCVAVRGAS+YV
Sbjct: 781  TGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAYHTHFVNVLGNPEKAYHNCVAVRGASVYV 840

Query: 841  LGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELVM 900
            LGPMHL+ISRLLPWKERVQVLRKAGDWM ALSMAITIYDG AHGVIDLPRSLESLQELV+
Sbjct: 841  LGPMHLIISRLLPWKERVQVLRKAGDWMGALSMAITIYDGQAHGVIDLPRSLESLQELVV 900

Query: 901  PFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVEF 960
            PFLIELLLSYVDEVFSYISVAFCNQIEKNEK  D+T   HSAHSEIKEQYNRVGGVAVEF
Sbjct: 901  PFLIELLLSYVDEVFSYISVAFCNQIEKNEKSVDVTSGGHSAHSEIKEQYNRVGGVAVEF 960

Query: 961  CVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGW 1020
            CVHI RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGW
Sbjct: 961  CVHIRRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKGW 1020

Query: 1021 LQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTSK 1080
            LQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVL+TSK
Sbjct: 1021 LQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLQTSK 1080

Query: 1081 SKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSIS 1140
            S+ AS++GYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD+VDTRSIS
Sbjct: 1081 SERASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDSVDTRSIS 1140

Query: 1141 SKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSIS 1200
            +KSSE+G L LY LLELDT ATLDVLRCAFVEGEI K  SS +G  D SMQ+QEEKNS S
Sbjct: 1141 NKSSEIGYLKLYHLLELDTGATLDVLRCAFVEGEIPKTNSSPNGLDDVSMQVQEEKNSTS 1200

Query: 1201 GRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVACG 1260
            GRKN LIQ++VDALVHVLD+AIC+TD S  G++I LV DWPSKK+L+HLFDF+A YVACG
Sbjct: 1201 GRKNVLIQSIVDALVHVLDQAICQTDRSAGGEDIALVGDWPSKKDLVHLFDFVANYVACG 1260

Query: 1261 KATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHWN 1320
             AT SK +VGQILEHLISNSDIPET +DFLP+VTANSV SRKREKQVLSLLEV+PETHWN
Sbjct: 1261 GATASKVIVGQILEHLISNSDIPETENDFLPKVTANSVHSRKREKQVLSLLEVVPETHWN 1320

Query: 1321 PSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSEQ 1380
             SSVL MCE+AQFFQVCGLIHSI HQY+SALDSYMKD+DEPIH F FINRTLLEL N E+
Sbjct: 1321 ASSVLSMCEEAQFFQVCGLIHSIRHQYASALDSYMKDIDEPIHGFAFINRTLLELNNYER 1380

Query: 1381 TEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVHL 1440
            TEF+AVVISRIPELFNLNR  TFFLVIDHFNND S+ILSQLR HPRSLFLYLKTLIEVHL
Sbjct: 1381 TEFQAVVISRIPELFNLNREGTFFLVIDHFNNDASDILSQLRTHPRSLFLYLKTLIEVHL 1440

Query: 1441 SGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDTIELYVELLCQH 1500
            SGS +FSCL+KDD  G+NYSTKG+DDYL+KLSDFPKYLSNNPVDVTDD IELYVELLCQ+
Sbjct: 1441 SGSLNFSCLRKDDIFGLNYSTKGLDDYLRKLSDFPKYLSNNPVDVTDDIIELYVELLCQY 1500

Query: 1501 ERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFHD 1560
            ERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKF+D
Sbjct: 1501 ERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFND 1560

Query: 1561 LEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQT 1620
            LEAAV + V+N ASSGSND ++FNSVLKLQEVN + VLLHACIGLCQRNTPRLNSEES+T
Sbjct: 1561 LEAAVESIVANGASSGSNDPRHFNSVLKLQEVNDIDVLLHACIGLCQRNTPRLNSEESET 1620

Query: 1621 LWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAHL 1680
            LWFKLLDSFCEPLIDSYN+RT +F +NQVQFLNESS  QKDKEA IVTWRILKS+K AH+
Sbjct: 1621 LWFKLLDSFCEPLIDSYNYRTPAFGENQVQFLNESSGLQKDKEARIVTWRILKSDKAAHI 1680

Query: 1681 LRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRILD 1740
            L++LFS+FI+EIVEGM+GYVHLP IMSRLL DNGSQEFGDFKLT+LGMLGTFGFERRILD
Sbjct: 1681 LKRLFSRFIKEIVEGMIGYVHLPIIMSRLLSDNGSQEFGDFKLTVLGMLGTFGFERRILD 1740

Query: 1741 SAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQ 1800
            +AKALIEDD+FYTMSLLKKGA+HGYAPR++VCCICN LL+KSSSSYRVRVFNCGHATHLQ
Sbjct: 1741 TAKALIEDDTFYTMSLLKKGASHGYAPRNLVCCICNCLLIKSSSSYRVRVFNCGHATHLQ 1800

Query: 1801 CEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQETD 1860
            CE L+NEASGGD++CP+C+HSNQSQ  + KA TEYSLVN FSS TQS SGASV YPQETD
Sbjct: 1801 CEVLDNEASGGDFSCPVCIHSNQSQRPRGKALTEYSLVNTFSSGTQSLSGASVQYPQETD 1819

Query: 1861 LLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESSG 1920
            LLELPYTLQQIPRFEILTNLQKNQRVI+IENVPQLRLAPPAVYHDKVTKGYHLLVGESS 
Sbjct: 1861 LLELPYTLQQIPRFEILTNLQKNQRVIEIENVPQLRLAPPAVYHDKVTKGYHLLVGESSS 1819

Query: 1921 GREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            G   +EK NKS+QL  VKVKRPSSLRFPLK SLFGKE+ T S
Sbjct: 1921 G---IEKQNKSKQLREVKVKRPSSLRFPLKASLFGKERTTKS 1819

BLAST of CSPI03G07210 vs. NCBI nr
Match: XP_011650623.1 (vacuolar protein sorting-associated protein 8 homolog [Cucumis sativus] >KGN56350.1 hypothetical protein Csa_010564 [Cucumis sativus])

HSP 1 Score: 3823.1 bits (9913), Expect = 0.0e+00
Identity = 1955/1963 (99.59%), Postives = 1956/1963 (99.64%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSS-GDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60
            MTEELTDTETLPPMELDLNAFIHAHLSS GDDDDDDDLSFPHRSIDEILNDSSSSTSPSP
Sbjct: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGGDDDDDDDLSFPHRSIDEILNDSSSSTSPSP 60

Query: 61   SSSPHFPPPRDRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTVSHSKVG 120
            SSSPHFPPPR RRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGT SHSKVG
Sbjct: 61   SSSPHFPPPRGRRNIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTASHSKVG 120

Query: 121  ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV 180
            ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV
Sbjct: 121  ELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAGYGNMV 180

Query: 181  LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN 240
            LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN
Sbjct: 181  LDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVSGELWATN 240

Query: 241  NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAEKNVKNV 300
            NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAE   KNV
Sbjct: 241  NIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPAE---KNV 300

Query: 301  CSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLA 360
            CSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLA
Sbjct: 301  CSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSKDTEVDLA 360

Query: 361  IEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEG 420
            IEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEG
Sbjct: 361  IEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFTALHWEEG 420

Query: 421  VAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMS 480
            VAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMS
Sbjct: 421  VAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHANYIAVGMS 480

Query: 481  KGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITV 540
            KGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITV
Sbjct: 481  KGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGYSDGHITV 540

Query: 541  WDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNR 600
            WDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNR
Sbjct: 541  WDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFSVVPLLNR 600

Query: 601  FSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSG 660
            FSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSG
Sbjct: 601  FSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGGVVGGDSG 660

Query: 661  WKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQS 720
            WKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQS
Sbjct: 661  WKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYTAWKCSQS 720

Query: 721  FETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILT 780
            FETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILT
Sbjct: 721  FETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDDQVLVILT 780

Query: 781  VTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIY 840
            VTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIY
Sbjct: 781  VTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVAVRGASIY 840

Query: 841  VLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELV 900
            VLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELV
Sbjct: 841  VLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSLESLQELV 900

Query: 901  MPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVE 960
            MPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVE
Sbjct: 901  MPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNRVGGVAVE 960

Query: 961  FCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKG 1020
            FCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKG
Sbjct: 961  FCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALVEHYSHKG 1020

Query: 1021 WLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTS 1080
            WLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTS
Sbjct: 1021 WLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEELLAVLRTS 1080

Query: 1081 KSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSI 1140
            KSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSI
Sbjct: 1081 KSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSDAVDTRSI 1140

Query: 1141 SSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSI 1200
            S+KSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSI
Sbjct: 1141 SNKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQLQEEKNSI 1200

Query: 1201 SGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVAC 1260
            SGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVAC
Sbjct: 1201 SGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDFIATYVAC 1260

Query: 1261 GKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHW 1320
            GKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHW
Sbjct: 1261 GKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLEVIPETHW 1320

Query: 1321 NPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSE 1380
            NPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSE
Sbjct: 1321 NPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTLLELGNSE 1380

Query: 1381 QTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVH 1440
            QTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVH
Sbjct: 1381 QTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYLKTLIEVH 1440

Query: 1441 LSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDTIELYVELLCQ 1500
            LSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDD IELYVELLCQ
Sbjct: 1441 LSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDIIELYVELLCQ 1500

Query: 1501 HERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFH 1560
            HERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFH
Sbjct: 1501 HERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLSSLDKKFH 1560

Query: 1561 DLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQ 1620
            DLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQ
Sbjct: 1561 DLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPRLNSEESQ 1620

Query: 1621 TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAH 1680
            TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAH
Sbjct: 1621 TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRILKSNKVAH 1680

Query: 1681 LLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRIL 1740
            LLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRIL
Sbjct: 1681 LLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTFGFERRIL 1740

Query: 1741 DSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHL 1800
            DSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHL
Sbjct: 1741 DSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFNCGHATHL 1800

Query: 1801 QCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQET 1860
            QCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQET
Sbjct: 1801 QCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGASVSYPQET 1860

Query: 1861 DLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESS 1920
            DLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESS
Sbjct: 1861 DLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYHLLVGESS 1920

Query: 1921 GGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            GGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS
Sbjct: 1921 GGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1960

BLAST of CSPI03G07210 vs. NCBI nr
Match: XP_008437780.1 (PREDICTED: vacuolar protein sorting-associated protein 8 homolog [Cucumis melo])

HSP 1 Score: 3603.1 bits (9342), Expect = 0.0e+00
Identity = 1854/1970 (94.11%), Postives = 1886/1970 (95.74%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDLSFPHRSIDEILNDSSSSTSPSPS 60
            MTEELTDT TLPPMELDLNAFIHAHLSSGDDDDDDDLSFPHRSIDEILNDSSSSTS SPS
Sbjct: 1    MTEELTDTRTLPPMELDLNAFIHAHLSSGDDDDDDDLSFPHRSIDEILNDSSSSTSSSPS 60

Query: 61   SSPHFPPPRDRRNIVAGDDGVSASPSTSPY--------KDSEAARNNPWNEKSAQLKPGT 120
            SSPH PP R RRNIVAG+ GVSASPSTSP+        KDSEA RNNPWNEKSAQ KPG 
Sbjct: 61   SSPHSPPSRGRRNIVAGNGGVSASPSTSPFKSLLEETIKDSEAPRNNPWNEKSAQSKPGK 120

Query: 121  VSHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRR 180
            VSHSK+GELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRR
Sbjct: 121  VSHSKIGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRR 180

Query: 181  AGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASMNVS 240
            AGYGNM LDDDELASSSAVDSEF SDSLYH NIH KESGENSISVVDRITDYQ+AS +VS
Sbjct: 181  AGYGNMALDDDELASSSAVDSEFLSDSLYHTNIHLKESGENSISVVDRITDYQVASRDVS 240

Query: 241  GELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLLGPA 300
             ELW  NNIRD VPHNDEFRMTEDMEFEAE SSVDDVNF ESL+TVPP ETNDRSLLGPA
Sbjct: 241  -ELWDRNNIRDSVPHNDEFRMTEDMEFEAEPSSVDDVNFNESLTTVPPAETNDRSLLGPA 300

Query: 301  EKNVKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQPSK 360
            E   KNVCSTDAHPTELDVDESNEGAIPR TEPDDE SAVGYGS ELETQDFEKYHQPSK
Sbjct: 301  E---KNVCSTDAHPTELDVDESNEGAIPRSTEPDDEGSAVGYGSPELETQDFEKYHQPSK 360

Query: 361  DTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQAFT 420
            DTEVDLAIEDPSIVNDIIESGETTEQ DNLQIGK PE + VSSTNPL+LAEEIEKKQAFT
Sbjct: 361  DTEVDLAIEDPSIVNDIIESGETTEQLDNLQIGKHPETMPVSSTNPLELAEEIEKKQAFT 420

Query: 421  ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN 480
            ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN
Sbjct: 421  ALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAVHAN 480

Query: 481  YIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLLAGY 540
            YIAVGMSKG+IVVVASKYSAQNGDNMDAKMILLGSQGDKSTAP TSLCFSQQ DLLLAGY
Sbjct: 481  YIAVGMSKGSIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPVTSLCFSQQADLLLAGY 540

Query: 541  SDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600
            SDGHITVWDVLRASAAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS
Sbjct: 541  SDGHITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLHTFS 600

Query: 601  VVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG 660
            VVPLLNRFS KTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG
Sbjct: 601  VVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSMMGG 660

Query: 661  VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSMPYT 720
            VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSP+VEVYAQLSKPDGIREGSMPYT
Sbjct: 661  VVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPSVEVYAQLSKPDGIREGSMPYT 720

Query: 721  AWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVWLDD 780
            AWKCSQSFETS SEAVERVSLLAIAWDKMVQVAKLVKTELKVCG WSLESAAIGVVWLDD
Sbjct: 721  AWKCSQSFETSSSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGNWSLESAAIGVVWLDD 780

Query: 781  QVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHNCVA 840
            QVLVILTVTGQLFLFEKDGTMIHQTS+F DGFVKEDFIAYHTHFAN+LG+PEKAYHNCVA
Sbjct: 781  QVLVILTVTGQLFLFEKDGTMIHQTSVFADGFVKEDFIAYHTHFANVLGHPEKAYHNCVA 840

Query: 841  VRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 900
            VRGASIYVLGP HLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL
Sbjct: 841  VRGASIYVLGPTHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLPRSL 900

Query: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQYNR 960
            ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+T E  SAHSEIKEQYNR
Sbjct: 901  ESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSERDSAHSEIKEQYNR 960

Query: 961  VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020
            VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV
Sbjct: 961  VGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQALV 1020

Query: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080
            EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL
Sbjct: 1021 EHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPLEEL 1080

Query: 1081 LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140
            LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD
Sbjct: 1081 LAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLENSD 1140

Query: 1141 AVDTRSISSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTSMQL 1200
            AVDTRSIS+KSSEVGCLNLY LLELDTEATLDVLRCAFVE E LK  SSLDGPVD  M+L
Sbjct: 1141 AVDTRSISNKSSEVGCLNLYHLLELDTEATLDVLRCAFVEVEFLKTNSSLDGPVDAIMEL 1200

Query: 1201 QEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHLFDF 1260
            Q+EKNSISGRKNFLIQNVVDALVHVL KAICETDESP GDNITLVDDWPSKKELIHLFDF
Sbjct: 1201 QDEKNSISGRKNFLIQNVVDALVHVLGKAICETDESPDGDNITLVDDWPSKKELIHLFDF 1260

Query: 1261 IATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLSLLE 1320
            IATYVACGKATVSKDVVGQILEHLISN+ IPET SDFLPRVTANSV SRKREKQVLSLLE
Sbjct: 1261 IATYVACGKATVSKDVVGQILEHLISNTHIPET-SDFLPRVTANSVHSRKREKQVLSLLE 1320

Query: 1321 VIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFINRTL 1380
            V+PETHWNPSSVLRMCEKAQFFQVCGLIHSI  QYSSALDSYMKDV EPIH F FINR L
Sbjct: 1321 VVPETHWNPSSVLRMCEKAQFFQVCGLIHSIGCQYSSALDSYMKDVGEPIHAFAFINRAL 1380

Query: 1381 LELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLFLYL 1440
            L+L NSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFN+DVSNIL QLRNHPRSLFLYL
Sbjct: 1381 LKLSNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNDDVSNILLQLRNHPRSLFLYL 1440

Query: 1441 KTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDTIEL 1500
            KTLIEVHLSGS DFSCLKKDDNLGVNYSTKG+DDYL+KLSDFPKYLSNNPVDVTDD IEL
Sbjct: 1441 KTLIEVHLSGSLDFSCLKKDDNLGVNYSTKGLDDYLKKLSDFPKYLSNNPVDVTDDIIEL 1500

Query: 1501 YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560
            YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS
Sbjct: 1501 YVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFLTLS 1560

Query: 1561 SLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRNTPR 1620
            SLDKKFHDLEAAVGA VSN ASSGS+DSQ+F+SVLKLQEVN V+VLLHACIGLCQRNTPR
Sbjct: 1561 SLDKKFHDLEAAVGAIVSNGASSGSSDSQHFDSVLKLQEVNTVEVLLHACIGLCQRNTPR 1620

Query: 1621 LNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTWRIL 1680
            LN EES+TLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNE S SQKDKEANIVTWRIL
Sbjct: 1621 LNCEESETLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNEPSSSQKDKEANIVTWRIL 1680

Query: 1681 KSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1740
            KSNK AH+LRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF
Sbjct: 1681 KSNKAAHILRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGMLGTF 1740

Query: 1741 GFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN 1800
            GFERRILD+AKALIEDDSFYTM+LLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN
Sbjct: 1741 GFERRILDTAKALIEDDSFYTMNLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVRVFN 1800

Query: 1801 CGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSSGAS 1860
            CGHATHLQCEDLENEASGGD TCPICVHSNQSQGSKSKAPTEYSLVNKFSSRT SSSGAS
Sbjct: 1801 CGHATHLQCEDLENEASGGDSTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTSSSSGAS 1860

Query: 1861 VSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH 1920
            VSYPQETD+LELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH
Sbjct: 1861 VSYPQETDILELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTKGYH 1920

Query: 1921 LLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            LLVGESS GREKVEKLNKSRQLT VKVKRPSSLRFPLK SLFGKEKMTNS
Sbjct: 1921 LLVGESSSGREKVEKLNKSRQLTEVKVKRPSSLRFPLKASLFGKEKMTNS 1965

BLAST of CSPI03G07210 vs. NCBI nr
Match: XP_038883953.1 (vacuolar protein sorting-associated protein 8 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 3426.3 bits (8883), Expect = 0.0e+00
Identity = 1761/1973 (89.25%), Postives = 1834/1973 (92.95%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSG---DDDDDDDLSFPHRSIDEILNDSSSSTSP 60
            MT+ELTD ETLP MELDLNAFIHAHLSSG   D DDDDDLSFPHRSIDEILN+SSSST  
Sbjct: 31   MTKELTDNETLPLMELDLNAFIHAHLSSGDGDDGDDDDDLSFPHRSIDEILNESSSST-- 90

Query: 61   SPSSSPHFPPPRDRRNIVAGDDGVSASPSTSPY--------KDSEAARNNPWNEKSAQLK 120
              SS PH PP R  RN  AGD GVS SPS SP+        KD E  R+N WNEKS QLK
Sbjct: 91   --SSPPHSPPSRGLRNNAAGDGGVSTSPSISPFKSPFEETIKDPETPRSNQWNEKSVQLK 150

Query: 121  PGTVSHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIK 180
            PG VSH+KVGELTDDPFRRGSRPLPSLFG VRSNAKPGAALAAAAAASRSTPAPHAAAIK
Sbjct: 151  PGAVSHAKVGELTDDPFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSTPAPHAAAIK 210

Query: 181  SRRAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASM 240
            SRRAGYGNMVLDDDEL SSSAV+SEFFSDSLYH NIHS++SGE SIS+VDRI DYQ+ASM
Sbjct: 211  SRRAGYGNMVLDDDELVSSSAVESEFFSDSLYHTNIHSEDSGEKSISMVDRIADYQVASM 270

Query: 241  NVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLL 300
            NV GELW  +NI DGVP+NDEF +T DME EAE SSVDDV+F ES +T+P V  N+RSL 
Sbjct: 271  NVGGELWERDNIGDGVPYNDEFLITRDMECEAEPSSVDDVDFNESATTLPRVGANNRSLS 330

Query: 301  GPAEKNVKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQ 360
            GP +   KNVCS + HPT LDVDESNEGAIP   EPDDE S+VGYGSLELETQDFEK  Q
Sbjct: 331  GPVQ---KNVCSMETHPTALDVDESNEGAIPWSIEPDDEGSSVGYGSLELETQDFEKCLQ 390

Query: 361  PSKDTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQ 420
            PSKDTEVDLAIEDPS V+DIIE GETTEQ DN QIG+RPE + +SSTNPL+LAEEIEKKQ
Sbjct: 391  PSKDTEVDLAIEDPSEVDDIIELGETTEQLDNFQIGERPERMPLSSTNPLELAEEIEKKQ 450

Query: 421  AFTALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAV 480
            AFTALHWEEGVAAQPMRLEGIKG TTTLGYFDIQADNSISRTISSHSF+REHGF QVLAV
Sbjct: 451  AFTALHWEEGVAAQPMRLEGIKGGTTTLGYFDIQADNSISRTISSHSFKREHGFSQVLAV 510

Query: 481  HANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLL 540
            HANYIAVGMSKGNIVVVASK SAQNGDNMDAKM+LLGSQGDKSTAP TSLCF+QQGDLLL
Sbjct: 511  HANYIAVGMSKGNIVVVASKSSAQNGDNMDAKMLLLGSQGDKSTAPVTSLCFNQQGDLLL 570

Query: 541  AGYSDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH 600
            AGYSDGHITVWDVLRASAAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH
Sbjct: 571  AGYSDGHITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH 630

Query: 601  TFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSM 660
            TFSVVPLLNRFS+KTQCLLDGQKTGTVLSASALLLNEF GSSLPPTLSN AVSTSSIGSM
Sbjct: 631  TFSVVPLLNRFSNKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPTLSNAAVSTSSIGSM 690

Query: 661  MGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSM 720
            MGGVVGGDSGWKLFNEGSS VEEGVV+FATHQTALVVRLSPTVEVYAQLSKPDGIREGSM
Sbjct: 691  MGGVVGGDSGWKLFNEGSSFVEEGVVVFATHQTALVVRLSPTVEVYAQLSKPDGIREGSM 750

Query: 721  PYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVW 780
            PYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVW
Sbjct: 751  PYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVW 810

Query: 781  LDDQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHN 840
            L+DQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGF KEDFIAYHTHF N+LGNPEKAYHN
Sbjct: 811  LNDQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFDKEDFIAYHTHFVNVLGNPEKAYHN 870

Query: 841  CVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLP 900
            CVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLP
Sbjct: 871  CVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLP 930

Query: 901  RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQ 960
            RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+  ESH AHSEIKEQ
Sbjct: 931  RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVMSESHYAHSEIKEQ 990

Query: 961  YNRVGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQ 1020
            YNRVGGVAVEFCVHI+RTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQ
Sbjct: 991  YNRVGGVAVEFCVHITRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQ 1050

Query: 1021 ALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL 1080
            ALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL
Sbjct: 1051 ALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL 1110

Query: 1081 EELLAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE 1140
            EELLAVLRTSKSK ASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE
Sbjct: 1111 EELLAVLRTSKSKRASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE 1170

Query: 1141 NSDAVDTRSISSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTS 1200
            NSD VDTRSIS+KSSEVG LNLY LLELDT ATLDVLRCAFVE E+LK  SS DG VD  
Sbjct: 1171 NSDPVDTRSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEDEVLKTNSSPDGSVDAR 1230

Query: 1201 MQLQEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHL 1260
            MQL+EEKNSISGRKNFLIQNVVD L+HVLDKAICETD S AGDNITLV++WPSKK+LIHL
Sbjct: 1231 MQLREEKNSISGRKNFLIQNVVDGLIHVLDKAICETDGSQAGDNITLVNNWPSKKDLIHL 1290

Query: 1261 FDFIATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLS 1320
            FDF+ATYVACGKAT SKDVVGQILEHLIS SDI ET SD LP VTANSV SRKREKQVLS
Sbjct: 1291 FDFVATYVACGKATASKDVVGQILEHLISKSDIAETTSDCLPHVTANSVHSRKREKQVLS 1350

Query: 1321 LLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFIN 1380
            LLEV+PETHWNPSSVLRMCEKAQFFQVCGLIHSI  QYSSALD YMKD+DEPIH F FIN
Sbjct: 1351 LLEVVPETHWNPSSVLRMCEKAQFFQVCGLIHSIRRQYSSALDGYMKDLDEPIHAFVFIN 1410

Query: 1381 RTLLELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLF 1440
            R LLEL NSE+TEFRAVVIS+IPELFNLNR ATFFLVIDHFNN+VS+ILSQL NHPRSLF
Sbjct: 1411 RALLELSNSERTEFRAVVISQIPELFNLNREATFFLVIDHFNNEVSDILSQLHNHPRSLF 1470

Query: 1441 LYLKTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDT 1500
            LYLKTLIEVHLSGS DFSCLKKD N GVNYSTKG+DDYLQKLSDFPKYLSNNPVDVTDD 
Sbjct: 1471 LYLKTLIEVHLSGSLDFSCLKKDGNFGVNYSTKGLDDYLQKLSDFPKYLSNNPVDVTDDI 1530

Query: 1501 IELYVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL 1560
            IELYV+LLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL
Sbjct: 1531 IELYVKLLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL 1590

Query: 1561 TLSSLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRN 1620
            TLSSLDKKFHDLEAAVG  V+N ASSGSNDSQ+F+SVLKLQEV AV+VLLHACIGLCQRN
Sbjct: 1591 TLSSLDKKFHDLEAAVGGIVTNGASSGSNDSQHFSSVLKLQEVKAVEVLLHACIGLCQRN 1650

Query: 1621 TPRLNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTW 1680
            TPRLNSEES+TLWFKLLDSFCEPLIDSYN+RTASFE+NQVQFLNESS SQK KEA+IVTW
Sbjct: 1651 TPRLNSEESETLWFKLLDSFCEPLIDSYNYRTASFEENQVQFLNESSASQKAKEAHIVTW 1710

Query: 1681 RILKSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGML 1740
            RILKSN+ AH+LR+LFSQFIREIVEGM GYVHLPTIMSRLLYDNGSQEFGDFKLTILGML
Sbjct: 1711 RILKSNEAAHILRRLFSQFIREIVEGMTGYVHLPTIMSRLLYDNGSQEFGDFKLTILGML 1770

Query: 1741 GTFGFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVR 1800
            GTFGFERRILD+AK LIEDD+FYTMSLLKKGA+HGYAP+SVVCCICNRLL+KSSSSYRVR
Sbjct: 1771 GTFGFERRILDTAKTLIEDDTFYTMSLLKKGASHGYAPQSVVCCICNRLLIKSSSSYRVR 1830

Query: 1801 VFNCGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSS 1860
            VFNCGHATHLQCED +NEASGGD+ CPICVHSNQSQ S+SKAP+EY LVNKFSSRTQSSS
Sbjct: 1831 VFNCGHATHLQCEDPDNEASGGDFICPICVHSNQSQRSRSKAPSEYGLVNKFSSRTQSSS 1890

Query: 1861 GASVSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTK 1920
            GASVSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPP VYHDKVTK
Sbjct: 1891 GASVSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPTVYHDKVTK 1950

Query: 1921 GYHLLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            GYHLLVGESS G+EKVE+LNKSRQLT VKVKRPSSLRFPLK SLFGKEK TNS
Sbjct: 1951 GYHLLVGESSSGKEKVERLNKSRQLTEVKVKRPSSLRFPLKASLFGKEKTTNS 1996

BLAST of CSPI03G07210 vs. NCBI nr
Match: XP_038883954.1 (vacuolar protein sorting-associated protein 8 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 3374.7 bits (8749), Expect = 0.0e+00
Identity = 1742/1973 (88.29%), Postives = 1813/1973 (91.89%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSG---DDDDDDDLSFPHRSIDEILNDSSSSTSP 60
            MT+ELTD ETLP MELDLNAFIHAHLSSG   D DDDDDLSFPHRSIDEILN+SSSST  
Sbjct: 31   MTKELTDNETLPLMELDLNAFIHAHLSSGDGDDGDDDDDLSFPHRSIDEILNESSSST-- 90

Query: 61   SPSSSPHFPPPRDRRNIVAGDDGVSASPSTSPY--------KDSEAARNNPWNEKSAQLK 120
              SS PH PP R  RN  AGD GVS SPS SP+        KD E  R+N WNEKS QLK
Sbjct: 91   --SSPPHSPPSRGLRNNAAGDGGVSTSPSISPFKSPFEETIKDPETPRSNQWNEKSVQLK 150

Query: 121  PGTVSHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIK 180
            PG VSH+KVGELTDDPFRRGSRPLPSLFG VRSNAKPGAALAAAAAASRSTPAPHAAAIK
Sbjct: 151  PGAVSHAKVGELTDDPFRRGSRPLPSLFGGVRSNAKPGAALAAAAAASRSTPAPHAAAIK 210

Query: 181  SRRAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASM 240
            SRRAGYGNMVLDDDEL SSSAV+SEFFSDSLYH NIHS++SGE SIS+VDRI DYQ+ASM
Sbjct: 211  SRRAGYGNMVLDDDELVSSSAVESEFFSDSLYHTNIHSEDSGEKSISMVDRIADYQVASM 270

Query: 241  NVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLL 300
            NV GELW  +NI DGVP+NDEF +T DME EAE SSVDDV+F ES +T+P V  N+RSL 
Sbjct: 271  NVGGELWERDNIGDGVPYNDEFLITRDMECEAEPSSVDDVDFNESATTLPRVGANNRSLS 330

Query: 301  GPAEKNVKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQ 360
            GP +   KNVCS + HPT LDVDESNEGAIP   EPDDE S+VGYGSLELETQDFEK  Q
Sbjct: 331  GPVQ---KNVCSMETHPTALDVDESNEGAIPWSIEPDDEGSSVGYGSLELETQDFEKCLQ 390

Query: 361  PSKDTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQ 420
            PSKDTEVDLAIEDPS V+DIIE GETTEQ DN QIG+RPE + +SSTNPL+LAEEIEKKQ
Sbjct: 391  PSKDTEVDLAIEDPSEVDDIIELGETTEQLDNFQIGERPERMPLSSTNPLELAEEIEKKQ 450

Query: 421  AFTALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAV 480
            AFTALHWEEGVAAQPMRLEGIKG TTTLGYFDIQADNSISRTISSHSF+REHGF QVLAV
Sbjct: 451  AFTALHWEEGVAAQPMRLEGIKGGTTTLGYFDIQADNSISRTISSHSFKREHGFSQVLAV 510

Query: 481  HANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLL 540
            HANYIAVGMSKGNIVVVASK SAQNGDNMDAK                      QGDLLL
Sbjct: 511  HANYIAVGMSKGNIVVVASKSSAQNGDNMDAK----------------------QGDLLL 570

Query: 541  AGYSDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH 600
            AGYSDGHITVWDVLRASAAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH
Sbjct: 571  AGYSDGHITVWDVLRASAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH 630

Query: 601  TFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSM 660
            TFSVVPLLNRFS+KTQCLLDGQKTGTVLSASALLLNEF GSSLPPTLSN AVSTSSIGSM
Sbjct: 631  TFSVVPLLNRFSNKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPTLSNAAVSTSSIGSM 690

Query: 661  MGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSM 720
            MGGVVGGDSGWKLFNEGSS VEEGVV+FATHQTALVVRLSPTVEVYAQLSKPDGIREGSM
Sbjct: 691  MGGVVGGDSGWKLFNEGSSFVEEGVVVFATHQTALVVRLSPTVEVYAQLSKPDGIREGSM 750

Query: 721  PYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVW 780
            PYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVW
Sbjct: 751  PYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVW 810

Query: 781  LDDQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHN 840
            L+DQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGF KEDFIAYHTHF N+LGNPEKAYHN
Sbjct: 811  LNDQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFDKEDFIAYHTHFVNVLGNPEKAYHN 870

Query: 841  CVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLP 900
            CVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLP
Sbjct: 871  CVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLP 930

Query: 901  RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQ 960
            RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+  ESH AHSEIKEQ
Sbjct: 931  RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVMSESHYAHSEIKEQ 990

Query: 961  YNRVGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQ 1020
            YNRVGGVAVEFCVHI+RTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQ
Sbjct: 991  YNRVGGVAVEFCVHITRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQ 1050

Query: 1021 ALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL 1080
            ALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL
Sbjct: 1051 ALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL 1110

Query: 1081 EELLAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE 1140
            EELLAVLRTSKSK ASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE
Sbjct: 1111 EELLAVLRTSKSKRASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE 1170

Query: 1141 NSDAVDTRSISSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTS 1200
            NSD VDTRSIS+KSSEVG LNLY LLELDT ATLDVLRCAFVE E+LK  SS DG VD  
Sbjct: 1171 NSDPVDTRSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEDEVLKTNSSPDGSVDAR 1230

Query: 1201 MQLQEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHL 1260
            MQL+EEKNSISGRKNFLIQNVVD L+HVLDKAICETD S AGDNITLV++WPSKK+LIHL
Sbjct: 1231 MQLREEKNSISGRKNFLIQNVVDGLIHVLDKAICETDGSQAGDNITLVNNWPSKKDLIHL 1290

Query: 1261 FDFIATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLS 1320
            FDF+ATYVACGKAT SKDVVGQILEHLIS SDI ET SD LP VTANSV SRKREKQVLS
Sbjct: 1291 FDFVATYVACGKATASKDVVGQILEHLISKSDIAETTSDCLPHVTANSVHSRKREKQVLS 1350

Query: 1321 LLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFIN 1380
            LLEV+PETHWNPSSVLRMCEKAQFFQVCGLIHSI  QYSSALD YMKD+DEPIH F FIN
Sbjct: 1351 LLEVVPETHWNPSSVLRMCEKAQFFQVCGLIHSIRRQYSSALDGYMKDLDEPIHAFVFIN 1410

Query: 1381 RTLLELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLF 1440
            R LLEL NSE+TEFRAVVIS+IPELFNLNR ATFFLVIDHFNN+VS+ILSQL NHPRSLF
Sbjct: 1411 RALLELSNSERTEFRAVVISQIPELFNLNREATFFLVIDHFNNEVSDILSQLHNHPRSLF 1470

Query: 1441 LYLKTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDT 1500
            LYLKTLIEVHLSGS DFSCLKKD N GVNYSTKG+DDYLQKLSDFPKYLSNNPVDVTDD 
Sbjct: 1471 LYLKTLIEVHLSGSLDFSCLKKDGNFGVNYSTKGLDDYLQKLSDFPKYLSNNPVDVTDDI 1530

Query: 1501 IELYVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL 1560
            IELYV+LLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL
Sbjct: 1531 IELYVKLLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL 1590

Query: 1561 TLSSLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRN 1620
            TLSSLDKKFHDLEAAVG  V+N ASSGSNDSQ+F+SVLKLQEV AV+VLLHACIGLCQRN
Sbjct: 1591 TLSSLDKKFHDLEAAVGGIVTNGASSGSNDSQHFSSVLKLQEVKAVEVLLHACIGLCQRN 1650

Query: 1621 TPRLNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTW 1680
            TPRLNSEES+TLWFKLLDSFCEPLIDSYN+RTASFE+NQVQFLNESS SQK KEA+IVTW
Sbjct: 1651 TPRLNSEESETLWFKLLDSFCEPLIDSYNYRTASFEENQVQFLNESSASQKAKEAHIVTW 1710

Query: 1681 RILKSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGML 1740
            RILKSN+ AH+LR+LFSQFIREIVEGM GYVHLPTIMSRLLYDNGSQEFGDFKLTILGML
Sbjct: 1711 RILKSNEAAHILRRLFSQFIREIVEGMTGYVHLPTIMSRLLYDNGSQEFGDFKLTILGML 1770

Query: 1741 GTFGFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVR 1800
            GTFGFERRILD+AK LIEDD+FYTMSLLKKGA+HGYAP+SVVCCICNRLL+KSSSSYRVR
Sbjct: 1771 GTFGFERRILDTAKTLIEDDTFYTMSLLKKGASHGYAPQSVVCCICNRLLIKSSSSYRVR 1830

Query: 1801 VFNCGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSS 1860
            VFNCGHATHLQCED +NEASGGD+ CPICVHSNQSQ S+SKAP+EY LVNKFSSRTQSSS
Sbjct: 1831 VFNCGHATHLQCEDPDNEASGGDFICPICVHSNQSQRSRSKAPSEYGLVNKFSSRTQSSS 1890

Query: 1861 GASVSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTK 1920
            GASVSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPP VYHDKVTK
Sbjct: 1891 GASVSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPTVYHDKVTK 1950

Query: 1921 GYHLLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            GYHLLVGESS G+EKVE+LNKSRQLT VKVKRPSSLRFPLK SLFGKEK TNS
Sbjct: 1951 GYHLLVGESSSGKEKVERLNKSRQLTEVKVKRPSSLRFPLKASLFGKEKTTNS 1974

BLAST of CSPI03G07210 vs. NCBI nr
Match: KAG6582725.1 (Vacuolar protein sorting-associated protein 8-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3300.0 bits (8555), Expect = 0.0e+00
Identity = 1705/1973 (86.42%), Postives = 1802/1973 (91.33%), Query Frame = 0

Query: 1    MTEELTDTETLPPMELDLNAFIHAHLSSGDDDDD---DDLSFPHRSIDEILNDSSSSTSP 60
            MT+ELTDTETLPPMELDLNAFIHAHLSSGDDDDD   DDLSFPHRSIDEILN+SSSSTS 
Sbjct: 1    MTKELTDTETLPPMELDLNAFIHAHLSSGDDDDDDDEDDLSFPHRSIDEILNESSSSTSS 60

Query: 61   SPSSSPHFPPPRDRRNIVAGDDGVSASPSTSPYKD--------SEAARNNPWNEKSAQLK 120
            SPSS P+ PPPRDRR+I A D   SAS S  P+K         S+  R+N  NEKS QLK
Sbjct: 61   SPSSPPNSPPPRDRRSIAARDGRASASRSIPPFKSPFEEIIKASKVPRSNQRNEKSVQLK 120

Query: 121  PGTVSHSKVGELTDDPFRRGSRPLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIK 180
            PG+ SH+KVGELTDDPFRRGSR LPSLFG VRSNAKPGAALAAAAAASRS PAPHAAAIK
Sbjct: 121  PGSASHTKVGELTDDPFRRGSRALPSLFGGVRSNAKPGAALAAAAAASRSMPAPHAAAIK 180

Query: 181  SRRAGYGNMVLDDDELASSSAVDSEFFSDSLYHANIHSKESGENSISVVDRITDYQIASM 240
            SRR+G+G++VLDDDELASSSAVDSEF  D LY    HSKES E SIS+V+R  DYQ AS+
Sbjct: 181  SRRSGHGSVVLDDDELASSSAVDSEFVFDDLYPTIDHSKESREKSISLVERNADYQGASV 240

Query: 241  NVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSLL 300
            NV GE WA +NIRD V +NDEFR+T+D E EAE S VDDVNF ES +T+PPVE N RSL 
Sbjct: 241  NVGGEFWARDNIRDCVLYNDEFRITKDTECEAEQSFVDDVNFDESSTTLPPVEANGRSLS 300

Query: 301  GPAEKNVKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYHQ 360
              A+    NVCS DA PT LDVDESNEGA P   +PD + SAVGYG LELETQDFEK  Q
Sbjct: 301  DSAD---NNVCSMDAEPTVLDVDESNEGAFPCSPKPDYDRSAVGYGKLELETQDFEKRFQ 360

Query: 361  PSKDTEVDLAIEDPSIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEEIEKKQ 420
            PSKD+EV LAIED S+VNDI ES ET EQ DN   G+R E +S+SS+NPL+LAEEIEKKQ
Sbjct: 361  PSKDSEV-LAIEDLSVVNDISESRETAEQLDNFHTGERAETMSLSSSNPLELAEEIEKKQ 420

Query: 421  AFTALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFPQVLAV 480
            AFTALHWEEGVAAQPMRLEGIKG TT LGYFDIQADNSISRTISSHSF+REHGFPQ LAV
Sbjct: 421  AFTALHWEEGVAAQPMRLEGIKGGTTALGYFDIQADNSISRTISSHSFKREHGFPQALAV 480

Query: 481  HANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQGDLLL 540
            HANYIAVGMSKGNIVVVASKYSAQNGDNMDAKM+LLGSQGDKS AP TSLCF+QQGDLLL
Sbjct: 481  HANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMLLLGSQGDKSNAPVTSLCFNQQGDLLL 540

Query: 541  AGYSDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH 600
            AGYSDG +TVWDVLRA+AAKVISGEH SPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH
Sbjct: 541  AGYSDGQVTVWDVLRATAAKVISGEHTSPVVHSLFLGQEAQVTRQFKAVTGDSKGLVLLH 600

Query: 601  TFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTSSIGSM 660
            TFSVVPLLNRFS KTQCLLDGQKTGTVLSASALLLNEF GSSLPP+LSNVAVSTSSIGSM
Sbjct: 601  TFSVVPLLNRFSIKTQCLLDGQKTGTVLSASALLLNEFCGSSLPPSLSNVAVSTSSIGSM 660

Query: 661  MGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGIREGSM 720
            MGGVVGGDSGWKLFNEGSSLV EGVVIFATHQTALVVRLSPTVEVYA+LSKPDGI+EGSM
Sbjct: 661  MGGVVGGDSGWKLFNEGSSLV-EGVVIFATHQTALVVRLSPTVEVYARLSKPDGIQEGSM 720

Query: 721  PYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVVW 780
            PYTAWKCSQS ETSPSEAVER+SLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGV W
Sbjct: 721  PYTAWKCSQSIETSPSEAVERISLLAIAWDKMVQVAKLVKTELKVCGKWSLESAAIGVAW 780

Query: 781  LDDQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPEKAYHN 840
            LDDQVLVILTVTGQLFLFEKDGTMIHQTS+FVDGF KEDFIA+HTHF N+LGNPEKAYHN
Sbjct: 781  LDDQVLVILTVTGQLFLFEKDGTMIHQTSVFVDGFDKEDFIAHHTHFVNVLGNPEKAYHN 840

Query: 841  CVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHGVIDLP 900
            CVAVRGAS+YVLGP HLVISRLLPWKERVQVLRKAGDWM+ALSMAITIYDGHAHGVIDLP
Sbjct: 841  CVAVRGASVYVLGPKHLVISRLLPWKERVQVLRKAGDWMTALSMAITIYDGHAHGVIDLP 900

Query: 901  RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHSEIKEQ 960
            RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDD+T  SHSAHSEIKEQ
Sbjct: 901  RSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDVTSGSHSAHSEIKEQ 960

Query: 961  YNRVGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLPPEIMQ 1020
            YNRVGGVAVEFCVHI+RTDILFDEIFSKFV VQQRDTFLELLEPYILKDMLGSLPPEIMQ
Sbjct: 961  YNRVGGVAVEFCVHITRTDILFDEIFSKFVAVQQRDTFLELLEPYILKDMLGSLPPEIMQ 1020

Query: 1021 ALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL 1080
            ALVEHYS KGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL
Sbjct: 1021 ALVEHYSQKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDDFRTPL 1080

Query: 1081 EELLAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE 1140
            EELLAVL+TSKSK AS++GYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE
Sbjct: 1081 EELLAVLQTSKSKRASAIGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELLQFLLE 1140

Query: 1141 NSDAVDTRSISSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDGPVDTS 1200
            NSD VDT+SIS+KSSEVG LNLY LLELDT ATLDVLRCAFVEGEI+KA SSLDG VD S
Sbjct: 1141 NSDTVDTKSISNKSSEVGYLNLYHLLELDTGATLDVLRCAFVEGEIVKADSSLDGSVDAS 1200

Query: 1201 MQLQEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDDWPSKKELIHL 1260
            MQ+Q+EKNS SGRKNFL+QNVVDAL+H+LDKAI +T  SP GDNITLV+DWPSKK+L HL
Sbjct: 1201 MQVQKEKNSTSGRKNFLVQNVVDALIHILDKAISQTYGSPGGDNITLVEDWPSKKDLFHL 1260

Query: 1261 FDFIATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRKREKQVLS 1320
            FDF+A YVACGKAT SKDVVGQILEHLISNSDIPE   D +  VTANSV SRKREKQVLS
Sbjct: 1261 FDFVANYVACGKATASKDVVGQILEHLISNSDIPEMEIDSVHSVTANSVHSRKREKQVLS 1320

Query: 1321 LLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPIHTFTFIN 1380
            LLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSI+HQYSSALD YMKDV+EPIH F FIN
Sbjct: 1321 LLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSISHQYSSALDGYMKDVEEPIHAFAFIN 1380

Query: 1381 RTLLELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLRNHPRSLF 1440
            RTL+EL  SEQTEFR VVISRIPEL NLNR  TFFLVIDHF NDVS+ILSQL NHPRSLF
Sbjct: 1381 RTLMELSYSEQTEFRGVVISRIPELLNLNREGTFFLVIDHFGNDVSDILSQLHNHPRSLF 1440

Query: 1441 LYLKTLIEVHLSGSPDFSCLKKDDNLGVNYSTKGMDDYLQKLSDFPKYLSNNPVDVTDDT 1500
            LYLKTLIEVH SG+ +FSCLKKDDN GVNYSTKG+DDYLQKLSDFPK+LSNNPVDVTDD 
Sbjct: 1441 LYLKTLIEVHQSGNLNFSCLKKDDNFGVNYSTKGLDDYLQKLSDFPKFLSNNPVDVTDDI 1500

Query: 1501 IELYVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL 1560
            IELYVELLCQ+ERESVLKFLETFDSY VEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL
Sbjct: 1501 IELYVELLCQYERESVLKFLETFDSYHVEHCLRLCQQYEVIDAAAFLLERVGDVGSALFL 1560

Query: 1561 TLSSLDKKFHDLEAAVGATVSNTASSGSNDSQNFNSVLKLQEVNAVKVLLHACIGLCQRN 1620
            TLSSLDKKFHDLEAAVG  V+N ASSGSNDSQ F+SVLKLQEVN + VLLHACIGLCQRN
Sbjct: 1561 TLSSLDKKFHDLEAAVGGIVTNGASSGSNDSQLFSSVLKLQEVNDIYVLLHACIGLCQRN 1620

Query: 1621 TPRLNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLNESSCSQKDKEANIVTW 1680
            TPRLNSEES+TLWFKLLDSFCEPLI+S+N+RTASF +NQVQFLNESS SQKDKEA+IVTW
Sbjct: 1621 TPRLNSEESETLWFKLLDSFCEPLIESFNYRTASFGENQVQFLNESSGSQKDKEAHIVTW 1680

Query: 1681 RILKSNKVAHLLRKLFSQFIREIVEGMMGYVHLPTIMSRLLYDNGSQEFGDFKLTILGML 1740
            RILKSN+ AH+LR LFS+FIREIVEGM+GYVHLPTIMSRLL DNGSQEFGDFKLTILGML
Sbjct: 1681 RILKSNQTAHILRSLFSRFIREIVEGMIGYVHLPTIMSRLLSDNGSQEFGDFKLTILGML 1740

Query: 1741 GTFGFERRILDSAKALIEDDSFYTMSLLKKGAAHGYAPRSVVCCICNRLLVKSSSSYRVR 1800
            GTFGFERRILD+AKALIEDD+FYTMSLLKKGA+HGYAPR  VCCICNRLLVKSSSSYRVR
Sbjct: 1741 GTFGFERRILDTAKALIEDDTFYTMSLLKKGASHGYAPRGAVCCICNRLLVKSSSSYRVR 1800

Query: 1801 VFNCGHATHLQCEDLENEASGGDYTCPICVHSNQSQGSKSKAPTEYSLVNKFSSRTQSSS 1860
            VFNCGHATHLQCE L+NEASG D++CP+CVHSN SQ S+ KA TEYSLVNKFSSRTQSSS
Sbjct: 1801 VFNCGHATHLQCEVLDNEASGADFSCPVCVHSNHSQRSRGKALTEYSLVNKFSSRTQSSS 1860

Query: 1861 GASVSYPQETDLLELPYTLQQIPRFEILTNLQKNQRVIDIENVPQLRLAPPAVYHDKVTK 1920
            GASVSYPQETDLLELPYTLQQIPRFEIL NLQKNQRVIDIEN+PQLRLAPPAVYHDKVTK
Sbjct: 1861 GASVSYPQETDLLELPYTLQQIPRFEILANLQKNQRVIDIENMPQLRLAPPAVYHDKVTK 1920

Query: 1921 GYHLLVGESSGGREKVEKLNKSRQLTGVKVKRPSSLRFPLKTSLFGKEKMTNS 1963
            GYHLLVG+SSGG EKVEKLNKSRQL  VKVKRPSSLRFPLK +LFGKEK T S
Sbjct: 1921 GYHLLVGDSSGGIEKVEKLNKSRQLKEVKVKRPSSLRFPLKANLFGKEKTTKS 1968

BLAST of CSPI03G07210 vs. TAIR 10
Match: AT4G00800.1 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 1828.9 bits (4736), Expect = 0.0e+00
Identity = 1054/1976 (53.34%), Postives = 1352/1976 (68.42%), Query Frame = 0

Query: 14   MELDLNAFIHAHLSSGDDDDD-DDLSFPHRSIDEILNDSSSSTSPSPSSSPHFPPPRDRR 73
            MELDL++F+   +S  D D D D  S PHR++DEILN SSSS+  + SS P  PP  +RR
Sbjct: 1    MELDLDSFL---VSDSDSDSDLDSSSVPHRTVDEILNASSSSS--ASSSPPSSPPSINRR 60

Query: 74   NIVAGDDGVSASPSTSPYKDSEAARNNPWNEKSAQLKPGTVSHSKVGELTDDPFRRGSR- 133
                 DD     P+    + SEA  N       A L+P +  H         P RR S  
Sbjct: 61   K---QDD-----PNR---RLSEALTN------VAVLRPESELHRGF-----PPTRRNSTS 120

Query: 134  -------PLPSLFGAVRSNAKPGAALAAAAAASRSTPAPHAAAIKSRRAG---------Y 193
                   PLPSL   VRSN KPGAALAAA AASR  P PHAA IKSRRA           
Sbjct: 121  SSSLRQLPLPSLLAGVRSNVKPGAALAAAVAASRLVPTPHAAIIKSRRASSASSELLLQV 180

Query: 194  GNMVLDDDELASSS------AVDSEFFSDSLYHANIHSKESGENSISVVDRITD-YQIAS 253
             N   DD E+ SS+      A  S    D          E  +N +S V  + D  ++  
Sbjct: 181  SNQEEDDHEVLSSNGDSVGVAAGSVSADDFRSFGGESLLEDEDNGVSGVASLEDEAKVME 240

Query: 254  MNVSGELWATNNIRDGVPHNDEFRMTEDMEFEAETSSVDDVNFKESLSTVPPVETNDRSL 313
            +  S    + N     V    +       E EAET+        E+ +     +T++  L
Sbjct: 241  VQASDITESLNPDLVTVSSGFDSEGNVSTEKEAETT-------MEAGNAAIDDDTDETML 300

Query: 314  LGPAEKNVKNVCSTDAHPTELDVDESNEGAIPRPTEPDDEESAVGYGSLELETQDFEKYH 373
            +    ++ ++   TD           +EG        +DEES+VG    ++++   +   
Sbjct: 301  VASLVESSESQHLTD-----------SEGKCDDAKVSNDEESSVG----DVKSDKSDIII 360

Query: 374  QPSKDTEVDLAIEDP----SIVNDIIESGETTEQPDNLQIGKRPEMISVSSTNPLDLAEE 433
              SK    D  I D     S +++++E  E   + +N ++ KR  + S S    L LAEE
Sbjct: 361  PESKKEGGDAFIPDDGSSMSGISELVE--ERIAELENERMSKRERLKSQSFRKQLVLAEE 420

Query: 434  IEKKQAFTALHWEEGVAAQPMRLEGIKGVTTTLGYFDIQADNSISRTISSHSFRREHGFP 493
             EKKQA+T LHWEEG AAQPMRLEG+K  +T LGYFD+ ADN ISRTISS +F+R+HG P
Sbjct: 421  FEKKQAYTGLHWEEGAAAQPMRLEGVKIGSTNLGYFDVDADNVISRTISSQAFKRDHGSP 480

Query: 494  QVLAVHANYIAVGMSKGNIVVVASKYSAQNGDNMDAKMILLGSQGDKSTAPATSLCFSQQ 553
            QVLAVH NYIAVG SKG IVVV SKYS+ + D M++KMI LG QG++S +P TS+CF+Q 
Sbjct: 481  QVLAVHLNYIAVGTSKGVIVVVPSKYSSDHADQMESKMIWLGLQGERSQSPVTSVCFNQI 540

Query: 554  GDLLLAGYSDGHITVWDVLRASAAKVISGEHASPVVHSLFLGQEAQVTRQFKAVTGDSKG 613
            G LLLAGY DGH+TVWD+ RAS AKVI+ EH +PVV++ FLG+++Q +RQFK +T D+KG
Sbjct: 541  GSLLLAGYGDGHVTVWDMQRASIAKVIT-EHTAPVVYAFFLGRDSQGSRQFKVITSDTKG 600

Query: 614  LVLLHTFSVVPLLNRFSSKTQCLLDGQKTGTVLSASALLLNEFVGSSLPPTLSNVAVSTS 673
            +V  H+FS   LLN ++ +TQCLLDGQK GTVLSAS L    F  S +     N AV +S
Sbjct: 601  VVFKHSFSYARLLNMYTVETQCLLDGQKNGTVLSASPLPDENFGSSLVSSKGGNSAVPSS 660

Query: 674  SIGSMMGGVVGGDSGWKLFNEGSSLVEEGVVIFATHQTALVVRLSPTVEVYAQLSKPDGI 733
            SI SMMGGVVG  S WKLFNE S+ VEEGVVIFAT+QT LVV+L P +EVYAQL +P+G+
Sbjct: 661  SISSMMGGVVGVGSTWKLFNEDSTSVEEGVVIFATYQTGLVVKLIPNLEVYAQLPRPEGV 720

Query: 734  REGSMPYTAWKCSQSFETSPSEAVERVSLLAIAWDKMVQVAKLVKTELKVCGKWSLESAA 793
            REGSMPYTAW+  +S E    EA +RVS L IAWD+ VQVAKLVK+++K   KWSL+S A
Sbjct: 721  REGSMPYTAWR--RSTENYSKEAEDRVSFLVIAWDRRVQVAKLVKSDIKEYAKWSLDSPA 780

Query: 794  IGVVWLDDQVLVILTVTGQLFLFEKDGTMIHQTSIFVDGFVKEDFIAYHTHFANILGNPE 853
            IGVVWLDDQ+LVI TVTG L+LF +DG +IHQT+  V G    D I+YHT+F N+ GNPE
Sbjct: 781  IGVVWLDDQLLVIPTVTGHLYLFTRDGVVIHQTNFSVAGSSGNDLISYHTYFTNVFGNPE 840

Query: 854  KAYHNCVAVRGASIYVLGPMHLVISRLLPWKERVQVLRKAGDWMSALSMAITIYDGHAHG 913
            KAYHN + VRGAS+Y+LG  HLVISRLLPWKERV VLR+ GDWM A +MA+++++G AHG
Sbjct: 841  KAYHNSMGVRGASVYILGTAHLVISRLLPWKERVDVLRRGGDWMGAFNMAMSLFNGQAHG 900

Query: 914  VIDLPRSLESLQELVMPFLIELLLSYVDEVFSYISVAFCNQIEKNEKLDDMTIESHSAHS 973
            V+DLP+++++++E + P L ELLLSYVDEVFSYIS+AF NQIE N    + +   ++ + 
Sbjct: 901  VVDLPKTVDAIREAIAPSLAELLLSYVDEVFSYISIAFSNQIENNGVTHEPSSGINNVNL 960

Query: 974  EIKEQYNRVGGVAVEFCVHISRTDILFDEIFSKFVGVQQRDTFLELLEPYILKDMLGSLP 1033
            EI+EQYNRVGGVAVEFCVHI+R D+LFDEIFS+FV VQQRDTFLELLEPYIL+DMLGSLP
Sbjct: 961  EIEEQYNRVGGVAVEFCVHINRMDLLFDEIFSRFVAVQQRDTFLELLEPYILRDMLGSLP 1020

Query: 1034 PEIMQALVEHYSHKGWLQRVEQCVLHMDISSLDFNQVVRLCRDHGLYSALVYLFNKGLDD 1093
            PEIMQALVEHYS KGWLQR+EQCVLHMDISSLDFNQVVR+CR+HGLY AL+YLFNKGLDD
Sbjct: 1021 PEIMQALVEHYSRKGWLQRIEQCVLHMDISSLDFNQVVRICREHGLYGALLYLFNKGLDD 1080

Query: 1094 FRTPLEELLAVLRTSKSKHASSLGYKTLVYLKYCFSGLAFPPGQGTLAHSRVQSLRDELL 1153
            FR+PLEELL VLR S+ + A+++GY+ LVYLKYCF GLAFPPG GTL  +R  SLR EL+
Sbjct: 1081 FRSPLEELLIVLRNSEKQRATAIGYRMLVYLKYCFLGLAFPPGHGTLNPTRWPSLRSELI 1140

Query: 1154 QFLLENSDAVDTRSISSKSSEVGCLNLYPLLELDTEATLDVLRCAFVEGEILKAISSLDG 1213
            QFLLE S+A D  S +  +S +  LNLY LLE+DTEATLDVLR AFVE E++K  S L  
Sbjct: 1141 QFLLEKSNAHD--SSTCVTSRLNYLNLYHLLEMDTEATLDVLRYAFVENEMVKHESHLLE 1200

Query: 1214 PVDTSMQLQEEKNSISGRKNFLIQNVVDALVHVLDKAICETDESPAGDNITLVDD--WPS 1273
              + S++ + + +      + LIQN+VDALVHV D  +     + +GD I    D  WPS
Sbjct: 1201 YGEVSVESKTDGSLPEVSNDILIQNLVDALVHVPDWGV----SNESGDPIDSKSDKNWPS 1260

Query: 1274 KKELIHLFDFIATYVACGKATVSKDVVGQILEHLISNSDIPETVSDFLPRVTANSVLSRK 1333
            K++  HLF+F+A Y A G+ ++SK V+ QIL++L S+  +P             +V S+ 
Sbjct: 1261 KEDTSHLFEFVAYYAARGRVSISKSVLAQILDYLTSDHILP-----------TYNVSSKM 1320

Query: 1334 REKQVLSLLEVIPETHWNPSSVLRMCEKAQFFQVCGLIHSITHQYSSALDSYMKDVDEPI 1393
            RE Q+L+LL+ +PET W+   V ++CEKA F+QVCG IH I  +Y +ALDSY+K+ DEPI
Sbjct: 1321 RENQLLNLLKAVPETDWDADYVSQLCEKAHFYQVCGYIHIIDRRYVAALDSYVKEADEPI 1380

Query: 1394 HTFTFINRTLLELGNSEQTEFRAVVISRIPELFNLNRGATFFLVIDHFNNDVSNILSQLR 1453
            H F ++N+ L +L   E T F++ +ISRIPEL +L+R   FFL+I +  + +  I  QL 
Sbjct: 1381 HLFCYVNKMLSQLSGDEFTAFQSAIISRIPELLDLSRQGAFFLIICNLKDTIKRIQEQLH 1440

Query: 1454 NHPRSLFLYLKTLIEVHLSGSPDFSCLKKD---DNLGVNYS---TKGMDDYLQKLSDFPK 1513
            +HPRSLFLYLKT+IEV+LSGS DFS L+K    D+ G N      K    YL+ L+DFPK
Sbjct: 1441 SHPRSLFLYLKTVIEVYLSGSLDFSRLRKHEAVDSSGENIRRDIPKEAKIYLEGLNDFPK 1500

Query: 1514 YLSNNPVDVTDDTIELYVELLCQHERESVLKFLETFDSYRVEHCLRLCQQYEVIDAAAFL 1573
            ++ +NPV+VTDD IELYVELLC++E +SVLKFLETFDSYRVEHCLRLCQ+Y ++DAAAFL
Sbjct: 1501 FIQDNPVNVTDDMIELYVELLCKYEPKSVLKFLETFDSYRVEHCLRLCQEYGIVDAAAFL 1560

Query: 1574 LERVGDVGSALFLTLSSLDKKFHDLEAAVGATVSNT---ASSGSNDSQNFNSVLKLQEVN 1633
            LERVGD GSAL LTLS L++K+ +LE AV   +S     AS G++  ++F+S L+L+EV+
Sbjct: 1561 LERVGDAGSALSLTLSGLNEKYVELEIAVECLMSEMKLGASEGAS-LEHFSSALELKEVH 1620

Query: 1634 AVKVLLHACIGLCQRNTPRLNSEESQTLWFKLLDSFCEPLIDSYNHRTASFEKNQVQFLN 1693
             ++ +L ACIGLCQRNTPRLN EES+ LWF+ LD+FCEPL++SY       E      +N
Sbjct: 1621 DIQGVLQACIGLCQRNTPRLNPEESEILWFRFLDTFCEPLMESYR------EPKNTDGIN 1680

Query: 1694 ESSCSQKDKEANI------VTWRILKSNKVA-HLLRKLFSQFIREIVEGMMGYVHLPTIM 1753
            + S   K  E ++      + WRI +S+  A H+LRKL SQFI+EIVEGM+GYV LPTIM
Sbjct: 1681 KGSLGVKSLERHVNESDVAIKWRIPRSDTAATHILRKLISQFIKEIVEGMIGYVRLPTIM 1740

Query: 1754 SRLLYDNGSQEFGDFKLTILGMLGTFGFERRILDSAKALIEDDSFYTMSLLKKGAAHGYA 1813
            ++LL DNG+QEFGDFKLTILGMLGT+GFERRILD+AK+LIEDD+FY+M+LLKKGA+HGYA
Sbjct: 1741 TKLLSDNGTQEFGDFKLTILGMLGTYGFERRILDTAKSLIEDDTFYSMNLLKKGASHGYA 1800

Query: 1814 PRSVVCCICNRLLVKSSSSYRVRVFNCGHATHLQCEDLENEASGGDYT-------CPICV 1873
            PRS++CCIC+  L K+ S+ RVRVFNCGHATHLQCE  ENE S    +       CP+C+
Sbjct: 1801 PRSLLCCICSCPLTKTFSALRVRVFNCGHATHLQCEPSENETSTSASSIHVSSSGCPVCM 1860

Query: 1874 HSNQSQGS-KSKA-PTEYSLVNKFSSRTQSSSGASVSYPQETDLLELPYTLQQIPRFEIL 1933
                S+ S K K+   +Y L++  SS   SS  AS  Y  E ++ +  +  QQ+ RFEIL
Sbjct: 1861 TKKTSKSSLKGKSFYRDYGLISTVSSNAGSSQRAS-PYSHENEMSDHSHN-QQLSRFEIL 1896