CSPI03G05660 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G05660
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Sesquiterpene synthase Tps1
Location	Chr3: 4787218 .. 4790683 (-)
RNA-Seq Expression	CSPI03G05660
Synteny	CSPI03G05660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGACGTGTGCATATGGTCAATCTACTACTTCCGGAGTACATGAAAGTTTATTATAGAACTGATCTGCTTGTTCTTTTTGAAGAGATGGATAAAGAGATAGTCAATGATGGAATATCCTATCGCTCTCCCTTTGCAAAAGAAGCTGTAAGGATTTTCTTTCTTCTAATAGTTTTTTCTATTGATATCCCTAATAATAATGGTATGTTTCTTATATGTATAACAAGTTTTAACTTTTTTTATGTGAATAGATGAAAAGACAAGCTGAATCCTATTTCAAAGAAGCTGGATGGCTAAACAAAAATTATAAACCAAAATATGAAGAATACATGGAAGTTGCTTTAACTACATCAGGTTATGAATTACTTTCAACTATTTCATTTATTTGCATGGGTGATATTGCAACAAAGGAAGTATTTGATTGGCTTTCTGATTGTCCCAAAATTCTCAAAGCCTCAAGCATCATTTGTAGAGCTCTTACAAGGTATATATGTGCACTTTCACTCCTTATTCAAAAAAGGCAGTGTATATATATTTTACTCTTTTTTCTTCTTTTATAGTTTGAGAAAGAAAGATAACATATTGTGTCTGCTGTTGAATGTTACATGGATAATTATGGATGTTCACAAGAAGAAACATGTACTGAGTTTCTTAAGCAGGTAGAAGATGCATGGAAGACTATAAATGAATCTTGTCTTCATCCGGTTATTGTTCCAATGCCATTTCTAATGTGTGTATTAAATCTCACAAGAGTTATGGCTCTACTTTATTCTGATGAAGATGGATATACAAATTCTAAGGGTAGGACTAAGCTTCTTATTCAGCCTTTACTTATCAATCCATAACCTTTGTAATTATTAATTGAAGTCTATGAATGACACCCTGAATTGCTAGCAGTGCTGGTATATCCTGAGTAGATTTTGTTGTTTTTTTAAAATTATTTAGAAATGGTGCTACATATTTAATTATTAAACTTTTTTCTCGAACAAAATTCCTAAAATGTCTTTAAACAATCCGGTTCCTATAACCATATAAGGACTGACAACATCAAAAGCTGAGAGTCAGAAGACACAAAAATTTGCAAATTAAATATTTTAAGGAAGAGATTTGTGAAAATCTTAACTAATAAGTAGTTTATCCTTTTTTGTAAAATAAATCTAGCTCAAATTTGTTAGTTACAACTTCTTCTAGCTTCTTCTTTAACTAAATATTTAACTAAAGCCTTAGGATAGATCTATAAATACATTTGTATCATTGGAAACAGCATATACTGAAATACAATCTTGCTCCCAAATTAGTACACAGTGGACGCAGATATCTTACTGAACCACTTTAACAAGCATCTTCTCCTACTTCTTCTCTTCGTCTACATCATCTCACCATCTTCTACAAATAAAGTTATCAGTAAAATCCATAAAAGAAACGCATAAAAGAAAGGAAAAGAAGAAAGAAAACTGAGGTTTCTTATTTCTCTAAAAAAAAGAGAAAGGTAACGTAACGACTCAACCCTTTACATTGACATGATAGAAAAAAGTAATAATAATAAGATTATTATTATTATTATTATTATTATTATTATATATATATAAAAAGTAGTTATACAAAAGAAATTAATAATAAGTTATTTTTTAAATATGTATATAGGTATATATAAAAGTAAAAGTAATAATAATAATAATAAAAGTTAAAAACAGAAAAGCAGAAGTAAAAGAAAGAGAAATATTAAAAAATGTTAATAACAAATAATAGATATATTAATAATAAGTAAAATAAGACATAAGAAACTTAAATAAGGTACATAATCTTTTATATATATATATATATATATTTTTAAAGAGAAAAATTAAAGATCAAGGTTAAAAAAAAAAGTCTTGTGTCTTTTTCTTTTCCTCTATTTGTTAGAGAGATGAAGAAAGACTAAGATTTTTTTAAAAAAGAAAAAACAACAATAAAAGTAAAAGAAAGCAAGAAAAACAATCATATATATTAACAAGATTTCATTTGCTAAAGAGGAATAAAAAAAAGTACGAAACAAAAAAAAAGGGCAAAGATACTAACGTAATGTAAAAAAACAATACCTTTTGCATAAACGTTTATTCCAATTGACAGATGTTCTCTTCAGAAAAAAATAATATAATAAAATAAAAAAATAAAACTTTCTCTAAAGATATTTTTTTTAAGAAATTTTTCTTCTCAAAGAATTCTTTGTTCAAGAAACCCTCTTTTTTTATTTTCAAATGACAAGAGTTCCTATTTATAGATTTCGAGTGTACCACAAATTAGGAAAAAGTAATAAGAATAGATTCAAATTTTGATATTATATTAATAGAATAAATTTTCATTTTCCTAAAATAAATTAAATGAGATATTTTGAGATTTTGTGAAAAATAAATTAATACATCAAATGGATATTAAAATATTTTTATTTCAAATTGATTTTCTTTTTTCCTAAAATGATTTAAGTAAGTTAAAATTGTATTTAAAACAAATTCATTGATTTTTTTCTAAAATAGTACAAACAAGATAAATGTGATATTAAATATTTTATTTAAAATAAATTGATTTATTTTTAATTTTTTAAAATAATAAAAATGAGATATTATAAGATGATATGTTATTACTATTTTTTTTACTATTTTTCAAAAATAAATAAATAAAAGTTTAGCAATTATTACTAATATTATTTTTAAAGAAAAATATTGGTAGGATAAAAATCCTTCATGCATCCTTGTAGAATTAGAAGGTGGACGAAGGTGTAGCCACTAACTCATCAAACTAAGATATTTTAAATAAATTACCAACGTCAAGGACCAAATATAAGATAAACATCTCATGACATGAAAAATAAGTTGGAAGTGAGGGATAAAACAAGCGTCATCATAGCAACATGGTGTTAAGGGCCAGAGACAAAATAAGCCTATCATGACAAAAGGATAAATAAGTTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGGGCCAGGGATAAAACAAGCTTCTTATGACAAAAGAATAAATAAGCTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGGGCCAGGGATAAAACAAGCTTCTTATGACAAAAGAATAAATAAGCTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGGGCCAGGGATAAAACAAGCTTCTTATGACAAAAGAATAAATAAGTTGTAGGTAGAGATAAAACAAATGTCACCACAGCAACATGGTATCAAGGATCAGGGATAAAACAAGCACCTCATGACAAAAGAATAAATAAGCTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGAGTCAGAGATAAAACAAGTGCCTCATGACAAAAGAATAAATAAGCTGGAGGTAGAGATAAAAGAAACGTCACCACAACAACATGGTGTCAAGGGCCAGGGATAA

mRNA sequence

ATGCGACGTGTGCATATGGTCAATCTACTACTTCCGGAGTACATGAAAGTTTATTATAGAACTGATCTGCTTGTTCTTTTTGAAGAGATGGATAAAGAGATAGTCAATGATGGAATATCCTATCGCTCTCCCTTTGCAAAAGAAGCTATGAAAAGACAAGCTGAATCCTATTTCAAAGAAGCTGGATGGCTAAACAAAAATTATAAACCAAAATATGAAGAATACATGGAAGTTGCTTTAACTACATCAGGTTATGAATTACTTTCAACTATTTCATTTATTTGCATGGGTGATATTGCAACAAAGGAAGTATTTGATTGGCTTTCTGATTGTCCCAAAATTCTCAAAGCCTCAAGCATCATTTGTAGAGCTCTTACAAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGGGCCAGGGATAAAACAAGCTTCTTATGACAAAAGAATAAATAAGCTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGGGCCAGGGATAAAACAAGCTTCTTATGACAAAAGAATAAATAAGCTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTCAACATGGTATCAAGGATCAGGGATAAAACAAGCACCTCATGACAAAAGAATAAATAAGCTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGAGTCAGAGATAAAACAAGTGCCTCATGACAAAAGAATAAATAAGCTGGAGGTAGAGATAAAAGAAACGTCACCACAACAACATGGTGTCAAGGGCCAGGGATAA

Coding sequence (CDS)

ATGCGACGTGTGCATATGGTCAATCTACTACTTCCGGAGTACATGAAAGTTTATTATAGAACTGATCTGCTTGTTCTTTTTGAAGAGATGGATAAAGAGATAGTCAATGATGGAATATCCTATCGCTCTCCCTTTGCAAAAGAAGCTATGAAAAGACAAGCTGAATCCTATTTCAAAGAAGCTGGATGGCTAAACAAAAATTATAAACCAAAATATGAAGAATACATGGAAGTTGCTTTAACTACATCAGGTTATGAATTACTTTCAACTATTTCATTTATTTGCATGGGTGATATTGCAACAAAGGAAGTATTTGATTGGCTTTCTGATTGTCCCAAAATTCTCAAAGCCTCAAGCATCATTTGTAGAGCTCTTACAAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGGGCCAGGGATAAAACAAGCTTCTTATGACAAAAGAATAAATAAGCTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGGGCCAGGGATAAAACAAGCTTCTTATGACAAAAGAATAAATAAGCTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTCAACATGGTATCAAGGATCAGGGATAAAACAAGCACCTCATGACAAAAGAATAAATAAGCTGGAGACCAGGGATAAAACAAGCGTCACCGCAGCAATACGGTGTCAAGAGTCAGAGATAAAACAAGTGCCTCATGACAAAAGAATAAATAAGCTGGAGGTAGAGATAAAAGAAACGTCACCACAACAACATGGTGTCAAGGGCCAGGGATAA

Protein sequence

MRRVHMVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRALTRDKTSVTAAIRCQGPGIKQASYDKRINKLETRDKTSVTAAIRCQGPGIKQASYDKRINKLETRDKTSVTAAIRSTWYQGSGIKQAPHDKRINKLETRDKTSVTAAIRCQESEIKQVPHDKRINKLEVEIKETSPQQHGVKGQG*

Homology

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BLAST of CSPI03G05660 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.8e-28
Identity = 62/115 (53.91%), Postives = 84/115 (73.04%), Query Frame = 0


Query: 11  LPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYKP 70
           LPEYMKV Y+  LL ++EE+++ +   G SYR  +AKEAMK+  ++Y  EA W+NKNY P
Sbjct: 335 LPEYMKVCYKA-LLNVYEEIERALSEQGRSYRLHYAKEAMKKLVQAYLVEANWMNKNYVP 394

Query: 71  KYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 126
             +EYM +AL +  Y LL+  SF+ MGDIATKEVFDW S+ PKI++ +SIICR +
Sbjct: 395 TMDEYMSIALVSCAYPLLTVTSFVGMGDIATKEVFDWASNDPKIVRVASIICRLM 448

[Close]

BLAST of CSPI03G05660 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.8e-26
Identity = 59/115 (51.30%), Postives = 81/115 (70.43%), Query Frame = 0


Query: 11  LPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYKP 70
           LPEYM+V Y+  LL ++ E+++E+  +G SYR  +AKEAMK Q  +Y++EA WL     P
Sbjct: 340 LPEYMRVCYQA-LLYVYSEIEEEMAKEGRSYRLYYAKEAMKNQVRAYYEEAKWLQVQQIP 399

Query: 71  KYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 126
             EEYM VAL TS Y +L+T SF+ MGD  TKE FDW+   PKI++AS+I+CR +
Sbjct: 400 TMEEYMPVALVTSAYSMLATTSFVGMGDAVTKESFDWIFSKPKIVRASAIVCRLM 453

[Close]

BLAST of CSPI03G05660 vs. ExPASy Swiss-Prot
Match: Q6Q3H2 (Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.8e-26
Identity = 63/136 (46.32%), Postives = 91/136 (66.91%), Query Frame = 0


Query: 11  LPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYKP 70
           LPEYMK+ Y   LL +++E+++E+  +G  YR  +AKE MK Q  +YF EA WL++ + P
Sbjct: 340 LPEYMKLCY-VALLDVYKEIEEEMEKEGNQYRVHYAKEVMKNQVRAYFAEAKWLHEEHVP 399

Query: 71  KYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRALTRDKT 130
            +EEYM VAL +SGY LL+T SF+ MG+IATKE FDW++  PKI+ +S+ I R +   K+
Sbjct: 400 AFEEYMRVALASSGYCLLATTSFVGMGEIATKEAFDWVTSDPKIMSSSNFITRLMDDIKS 459

Query: 131 --------SVTAAIRC 139
                    VT+A+ C
Sbjct: 460 HKFEQKRGHVTSAVEC 474

[Close]

BLAST of CSPI03G05660 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 7.0e-25
Identity = 65/137 (47.45%), Postives = 90/137 (65.69%), Query Frame = 0


Query: 11  LPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYKP 70
           LP YMK +Y   +L L+EE+ KEI  D  S     A   +KR +ESYF+EA WLNK YKP
Sbjct: 353 LPLYMKPFY-VAMLELYEEIGKEIDKDQNSLHLQVAIGGIKRLSESYFEEAKWLNKEYKP 412

Query: 71  KYEEYMEVALTTSGYELLSTISFICMGD-IATKEVFDWLSDCPKILKASSIICRAL---- 130
            ++EYME+AL T+GY +L +ISF+ +GD I T EV  WLS+ P+I+KAS+IICR +    
Sbjct: 413 SFKEYMELALKTTGYTMLISISFLGLGDHIVTNEVLQWLSNGPQIIKASTIICRLMDDIA 472

Query: 131 ----TRDKTSVTAAIRC 139
                +++  V +A+ C
Sbjct: 473 SHKFEQEREHVASAVEC 488

[Close]

BLAST of CSPI03G05660 vs. ExPASy Swiss-Prot
Match: B5A435 (Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.2e-24
Identity = 55/115 (47.83%), Postives = 80/115 (69.57%), Query Frame = 0


Query: 11  LPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYKP 70
           LPEYMK  Y++ +L ++ E+++E+ N G  +R  +AKE MK+  E Y  EA W ++ Y P
Sbjct: 342 LPEYMKPCYKS-ILDVYNEIEEEMDNQGSLFRMHYAKEVMKKLVEGYMDEAKWCHEKYVP 401

Query: 71  KYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 126
            +EEYM VAL TSGY  L+TIS++ MG+IA+KE FDWL   P +++AS  +CR +
Sbjct: 402 TFEEYMPVALVTSGYTFLTTISYLGMGEIASKEAFDWLFSHPPVIEASESVCRLM 455

[Close]

BLAST of CSPI03G05660 vs. ExPASy TrEMBL
Match: A0A0A0L337 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G096040 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.6e-61
Identity = 124/126 (98.41%), Postives = 124/126 (98.41%), Query Frame = 0


Query: 1   MRRVHMVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKE 60
           MRRVHMVNLLLPEYMKVYYRTDLL LFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKE
Sbjct: 124 MRRVHMVNLLLPEYMKVYYRTDLLDLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKE 183

Query: 61  AGWLNKNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSI 120
           AGWLNKNYKPKYEEYMEVALTTSGYELLSTISFI MGDIATKEVFDWLSDCPKILKASSI
Sbjct: 184 AGWLNKNYKPKYEEYMEVALTTSGYELLSTISFIGMGDIATKEVFDWLSDCPKILKASSI 243

Query: 121 ICRALT 127
           ICRALT
Sbjct: 244 ICRALT 249

[Close]

BLAST of CSPI03G05660 vs. ExPASy TrEMBL
Match: A0A1S4DZR2 ((+)-gamma-cadinene synthase-like OS=Cucumis melo OX=3656 GN=LOC103493390 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 2.2e-45
Identity = 101/141 (71.63%), Postives = 115/141 (81.56%), Query Frame = 0


Query: 6   MVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN 65
           M+N LLP+YMKVYY T LL LFEE+DKEIVND  SYRS FAKEAMKRQAESYFKEA WLN
Sbjct: 349 MIN-LLPKYMKVYY-TTLLDLFEEIDKEIVNDETSYRSHFAKEAMKRQAESYFKEAEWLN 408

Query: 66  KNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 125
           KNYKPKYEEYMEVAL +SGYELLSTISF+CMGDIAT+E+FDWL DCPKILKAS+ I R +
Sbjct: 409 KNYKPKYEEYMEVALASSGYELLSTISFVCMGDIATEEIFDWLFDCPKILKASTTISRLM 468

Query: 126 --------TRDKTSVTAAIRC 139
                    +++  V +A+ C
Sbjct: 469 DDVVSYKFEKEREHVVSAVEC 487

[Close]

BLAST of CSPI03G05660 vs. ExPASy TrEMBL
Match: A0A0A0L579 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G097040 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.8e-45
Identity = 102/141 (72.34%), Postives = 115/141 (81.56%), Query Frame = 0


Query: 6   MVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN 65
           MVN LLP+YMKV+Y T LL LFEEMDK IVNDGISYRS FAKEAMKRQAESYFKEA WLN
Sbjct: 346 MVN-LLPQYMKVHY-TTLLDLFEEMDKGIVNDGISYRSCFAKEAMKRQAESYFKEAEWLN 405

Query: 66  KNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 125
           KNYKPKY EYMEVAL +SGYELLSTISF+CMGDIATKEVF+WL DCPKILKAS+ I R +
Sbjct: 406 KNYKPKYGEYMEVALASSGYELLSTISFVCMGDIATKEVFEWLFDCPKILKASTTISRLM 465

Query: 126 --------TRDKTSVTAAIRC 139
                    +++  + +A+ C
Sbjct: 466 DDVVSYKFEKEREHIVSAVEC 484

[Close]

BLAST of CSPI03G05660 vs. ExPASy TrEMBL
Match: A0A0B4VEV4 (Sesquiterpene synthase Tps1 OS=Cucumis melo OX=3656 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 3.7e-45
Identity = 101/141 (71.63%), Postives = 114/141 (80.85%), Query Frame = 0


Query: 6   MVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN 65
           M+N LLP+YMKVYY T LL LFEE+DKEIVND  SYR  FAKEAMKRQAESYFKEA WLN
Sbjct: 349 MIN-LLPKYMKVYY-TTLLDLFEEIDKEIVNDETSYRFHFAKEAMKRQAESYFKEAEWLN 408

Query: 66  KNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 125
           KNYKPKYEEYMEVAL +SGYELLSTISF+CMGDIATKE+FDWL DCPKILKAS+ I R +
Sbjct: 409 KNYKPKYEEYMEVALASSGYELLSTISFVCMGDIATKEIFDWLFDCPKILKASTTISRLM 468

Query: 126 --------TRDKTSVTAAIRC 139
                    +++  V +A+ C
Sbjct: 469 DDVVSYKFEKEREHVVSAVEC 487

[Close]

BLAST of CSPI03G05660 vs. ExPASy TrEMBL
Match: Q66PX8 (Beta-caryophyllene synthase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 2.4e-44
Identity = 100/141 (70.92%), Postives = 114/141 (80.85%), Query Frame = 0


Query: 6   MVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN 65
           MVN LLP+YMKV+Y T LL LFEEMDK IVNDGISYRS F KEAMKRQAESYFKEA WLN
Sbjct: 346 MVN-LLPQYMKVHY-TTLLDLFEEMDKGIVNDGISYRSCFGKEAMKRQAESYFKEAEWLN 405

Query: 66  KNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 125
           KNYKPKY EYMEVAL +SGYELLSTISF+CMGDIATKEVF+WL DCP+ILKAS+ I R +
Sbjct: 406 KNYKPKYGEYMEVALASSGYELLSTISFVCMGDIATKEVFEWLFDCPQILKASTTISRLM 465

Query: 126 --------TRDKTSVTAAIRC 139
                    +++  + +A+ C
Sbjct: 466 DDVVSYKFEKEREHIVSAVEC 484

[Close]

BLAST of CSPI03G05660 vs. NCBI nr
Match: KAE8650156.1 (hypothetical protein Csa_010514 [Cucumis sativus])

HSP 1 Score: 246.1 bits (627), Expect = 3.4e-61
Identity = 124/126 (98.41%), Postives = 124/126 (98.41%), Query Frame = 0


Query: 1   MRRVHMVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKE 60
           MRRVHMVNLLLPEYMKVYYRTDLL LFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKE
Sbjct: 204 MRRVHMVNLLLPEYMKVYYRTDLLDLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKE 263

Query: 61  AGWLNKNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSI 120
           AGWLNKNYKPKYEEYMEVALTTSGYELLSTISFI MGDIATKEVFDWLSDCPKILKASSI
Sbjct: 264 AGWLNKNYKPKYEEYMEVALTTSGYELLSTISFIGMGDIATKEVFDWLSDCPKILKASSI 323

Query: 121 ICRALT 127
           ICRALT
Sbjct: 324 ICRALT 329

[Close]

BLAST of CSPI03G05660 vs. NCBI nr
Match: XP_031738839.1 (LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like [Cucumis sativus])

HSP 1 Score: 238.0 bits (606), Expect = 9.2e-59
Identity = 120/122 (98.36%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 6   MVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN 65
           MVNLLLPEYMKVYYRTDLL LFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN
Sbjct: 1   MVNLLLPEYMKVYYRTDLLDLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN 60

Query: 66  KNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 125
           KNYKPKYEEYMEVALTTSGYELLSTISFI MGDIATKEVFDWLSDCPKILKASSIICRAL
Sbjct: 61  KNYKPKYEEYMEVALTTSGYELLSTISFIGMGDIATKEVFDWLSDCPKILKASSIICRAL 120

Query: 126 TR 128
           TR
Sbjct: 121 TR 122

[Close]

BLAST of CSPI03G05660 vs. NCBI nr
Match: KAE8650158.1 (hypothetical protein Csa_009547 [Cucumis sativus])

HSP 1 Score: 192.2 bits (487), Expect = 5.8e-45
Identity = 102/141 (72.34%), Postives = 115/141 (81.56%), Query Frame = 0


Query: 6   MVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN 65
           MVN LLP+YMKV+Y T LL LFEEMDK IVNDGISYRS FAKEAMKRQAESYFKEA WLN
Sbjct: 346 MVN-LLPQYMKVHY-TTLLDLFEEMDKGIVNDGISYRSCFAKEAMKRQAESYFKEAEWLN 405

Query: 66  KNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 125
           KNYKPKY EYMEVAL +SGYELLSTISF+CMGDIATKEVF+WL DCPKILKAS+ I R +
Sbjct: 406 KNYKPKYGEYMEVALASSGYELLSTISFVCMGDIATKEVFEWLFDCPKILKASTTISRLM 465

Query: 126 --------TRDKTSVTAAIRC 139
                    +++  + +A+ C
Sbjct: 466 DDVVSYKFEKEREHIVSAVEC 484

[Close]

BLAST of CSPI03G05660 vs. NCBI nr
Match: NP_001315388.1 ((+)-gamma-cadinene synthase-like [Cucumis melo] >AJD19682.1 sesquiterpene synthase Tps1 [Cucumis melo])

HSP 1 Score: 191.8 bits (486), Expect = 7.6e-45
Identity = 101/141 (71.63%), Postives = 114/141 (80.85%), Query Frame = 0


Query: 6   MVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN 65
           M+N LLP+YMKVYY T LL LFEE+DKEIVND  SYR  FAKEAMKRQAESYFKEA WLN
Sbjct: 349 MIN-LLPKYMKVYY-TTLLDLFEEIDKEIVNDETSYRFHFAKEAMKRQAESYFKEAEWLN 408

Query: 66  KNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 125
           KNYKPKYEEYMEVAL +SGYELLSTISF+CMGDIATKE+FDWL DCPKILKAS+ I R +
Sbjct: 409 KNYKPKYEEYMEVALASSGYELLSTISFVCMGDIATKEIFDWLFDCPKILKASTTISRLM 468

Query: 126 --------TRDKTSVTAAIRC 139
                    +++  V +A+ C
Sbjct: 469 DDVVSYKFEKEREHVVSAVEC 487

[Close]

BLAST of CSPI03G05660 vs. NCBI nr
Match: NP_001292628.1 ((+)-gamma-cadinene synthase [Cucumis sativus] >AAU05952.1 beta-caryophyllene synthase [Cucumis sativus])

HSP 1 Score: 189.1 bits (479), Expect = 4.9e-44
Identity = 100/141 (70.92%), Postives = 114/141 (80.85%), Query Frame = 0


Query: 6   MVNLLLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLN 65
           MVN LLP+YMKV+Y T LL LFEEMDK IVNDGISYRS F KEAMKRQAESYFKEA WLN
Sbjct: 346 MVN-LLPQYMKVHY-TTLLDLFEEMDKGIVNDGISYRSCFGKEAMKRQAESYFKEAEWLN 405

Query: 66  KNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICRAL 125
           KNYKPKY EYMEVAL +SGYELLSTISF+CMGDIATKEVF+WL DCP+ILKAS+ I R +
Sbjct: 406 KNYKPKYGEYMEVALASSGYELLSTISFVCMGDIATKEVFEWLFDCPQILKASTTISRLM 465

Query: 126 --------TRDKTSVTAAIRC 139
                    +++  + +A+ C
Sbjct: 466 DDVVSYKFEKEREHIVSAVEC 484

[Close]

BLAST of CSPI03G05660 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 78.6 bits (192), Expect = 8.7e-15
Identity = 41/113 (36.28%), Postives = 68/113 (60.18%), Query Frame = 0


Query: 11  LPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYKP 70
           +P+ MK  Y    +  ++++D+E+  +G S      K+++++ A  Y +EA WL K+Y  
Sbjct: 331 IPDSMKFIYNV-TVEFYDKLDEELEKEGRSGCGFHLKKSLQKTANGYMQEAKWLKKDYIA 390

Query: 71  KYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICR 124
            ++EY E A+ +SGY  L  ++F+ M D+A  + F+WLS  PKI  AS II R
Sbjct: 391 TFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEIISR 442

[Close]

BLAST of CSPI03G05660 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 78.6 bits (192), Expect = 8.7e-15
Identity = 41/113 (36.28%), Postives = 68/113 (60.18%), Query Frame = 0


Query: 11  LPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYKP 70
           +P+ MK  Y    +  ++++D+E+  +G S      K+++++ A  Y +EA WL K+Y  
Sbjct: 329 IPDSMKFIYNV-TVEFYDKLDEELEKEGRSGCGFHLKKSLQKTANGYMQEAKWLKKDYIA 388

Query: 71  KYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICR 124
            ++EY E A+ +SGY  L  ++F+ M D+A  + F+WLS  PKI  AS II R
Sbjct: 389 TFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEIISR 440

[Close]

BLAST of CSPI03G05660 vs. TAIR 10
Match: AT1G70080.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 3.3e-14
Identity = 38/108 (35.19%), Postives = 68/108 (62.96%), Query Frame = 0


Query: 10  LLPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYK 69
           +LP+Y+KV +RT    LF+E+++ + ++  S+   +A E ++   + Y +EA W N+ + 
Sbjct: 392 VLPDYLKVVFRT-TFDLFKELEEYVSSEARSFTMQYAYEQLRILMKGYLQEAEWSNRGHL 451

Query: 70  PKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKA 118
           P +EEY+EV + ++  E+L  ++FI MGD A   V++WL   PK+  A
Sbjct: 452 PSHEEYIEVGVASTAGEVLLAMTFIPMGDAAGVGVYEWLRSRPKLTHA 498

[Close]

BLAST of CSPI03G05660 vs. TAIR 10
Match: AT5G48110.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 5.9e-11
Identity = 37/113 (32.74%), Postives = 60/113 (53.10%), Query Frame = 0


Query: 11  LPEYMKVYYRTDLLVLFEEMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGWLNKNYKP 70
           +P++MKV  R+ L+ L E+   E+ ++G  Y   +  +  KR   +    + W    Y P
Sbjct: 361 MPDHMKVLLRS-LIDLMEDFKGEVRSEGRLYSVEYGIDEWKRLFRADLTISKWARTGYIP 420

Query: 71  KYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICR 124
            Y+EYMEV + T G ++    +FI MG+ A KE FDW+   PK ++   +  R
Sbjct: 421 NYDEYMEVGIVTGGVDVTVAFAFIGMGE-AGKEAFDWIRSRPKFIQTIDLKSR 471

[Close]

BLAST of CSPI03G05660 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 7.2e-09
Identity = 38/120 (31.67%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 7   VNLLLPEYMKVYYRTDLLVLFE---EMDKEIVNDGISYRSPFAKEAMKRQAESYFKEAGW 66
           +N+   + +  Y R  L  +FE   E+++E+   G SY      E  K  A++Y +   W
Sbjct: 375 LNIGADDKLSGYLRIVLESVFEVMGEIEQEMSPKGRSYSVKQVLERFKIIAKAYKQLTEW 434

Query: 67  LNKNYKPKYEEYMEVALTTSGYELLSTISFICMGDIATKEVFDWLSDCPKILKASSIICR 124
             K + P ++EYM+V L T+G    +   FI M DI  KE F+WL+  P ++ A +++ R
Sbjct: 435 ARKGHVPTFDEYMKVGLVTAGMGDYAGYCFIGMEDINEKEAFEWLNSNPLLIDALNVLFR 494

The following BLAST results are available for this feature:

BLAST of CSPI03G05660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9SCB6	1.8e-28	53.91	Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1	[more]
Q6Q3H3	2.8e-26	51.30	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]
Q6Q3H2	4.8e-26	46.32	Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1	[more]
B2KSJ5	7.0e-25	47.45	(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
B5A435	1.2e-24	47.83	Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1	[more]

BLAST of CSPI03G05660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L337	1.6e-61	98.41	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G096040 PE=4 SV=1	[more]
A0A1S4DZR2	2.2e-45	71.63	(+)-gamma-cadinene synthase-like OS=Cucumis melo OX=3656 GN=LOC103493390 PE=4 SV...	[more]
A0A0A0L579	2.8e-45	72.34	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G097040 PE=4 SV=1	[more]
A0A0B4VEV4	3.7e-45	71.63	Sesquiterpene synthase Tps1 OS=Cucumis melo OX=3656 PE=2 SV=1	[more]
Q66PX8	2.4e-44	70.92	Beta-caryophyllene synthase OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]

BLAST of CSPI03G05660 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8650156.1	3.4e-61	98.41	hypothetical protein Csa_010514 [Cucumis sativus]	[more]
XP_031738839.1	9.2e-59	98.36	LOW QUALITY PROTEIN: (+)-gamma-cadinene synthase-like [Cucumis sativus]	[more]
KAE8650158.1	5.8e-45	72.34	hypothetical protein Csa_009547 [Cucumis sativus]	[more]
NP_001315388.1	7.6e-45	71.63	(+)-gamma-cadinene synthase-like [Cucumis melo] >AJD19682.1 sesquiterpene syntha...	[more]
NP_001292628.1	4.9e-44	70.92	(+)-gamma-cadinene synthase [Cucumis sativus] >AAU05952.1 beta-caryophyllene syn...	[more]

BLAST of CSPI03G05660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23960.1	8.7e-15	36.28	terpene synthase 21	[more]
AT5G23960.2	8.7e-15	36.28	terpene synthase 21	[more]
AT1G70080.1	3.3e-14	35.19	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT5G48110.1	5.9e-11	32.74	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G14540.1	7.2e-09	31.67	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 4..139 e-value: 1.1E-32 score: 115.2
IPR008949	Isoprenoid synthase domain superfamily	SUPERFAMILY	48576	Terpenoid synthases	coord: 10..123
IPR005630	Terpene synthase, metal-binding domain	PFAM	PF03936	Terpene_synth_C	coord: 10..125 e-value: 1.1E-29 score: 103.5
None	No IPR available	PANTHER	PTHR31225:SF134	(-)-GERMACRENE D SYNTHASE-LIKE	coord: 8..126
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 8..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G05660.1	CSPI03G05660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0016829	lyase activity