CSPI03G05580 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G05580
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	exosome complex exonuclease RRP44 homolog A
Location	Chr3: 4727235 .. 4730421 (+)
RNA-Seq Expression	CSPI03G05580
Synteny	CSPI03G05580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATATATATTGTGGGCTTCGCTTTGCAAAAATGTGAGTCTTCAGTTGCTTATTTGGGTGCTTCTGCATCGCCAATTCTAGTTCTCGACACCAATGTTGTTCTTACTCAGGTTATTTTCGTCTGGTTTCTGTTGTCAACATTGAGCTTTCAACTTTTAGTGTTTTATGAAATGATGCGTTTTTACAGATTGGTTTGCTTGAGAATTCTGCTATTGATGACGTGGTTATGCTATCAGTTGTGCTGGATGAGGTTAAGAACAAGAACCTATCTGTTTACAACAGAGTTAGAGCTCTCTGCAGCAATCCCCTGTGGAGATTCTTTGTTTTCTTTAACGAGCACCACAAGTATGCTTTCTATATATGCTTTCTATTTACACTTTCGTTATTGCTGCCTTTCAGAGTCATCTCTAAATAATATTGTACTATTTAATAAACAACAGGATAAATTCACACTTGGCTTGGAGATTCAAAATCTTTCATCTTTTATTGTATTTCTGAACTACATGAAAATTTGGGATATTGCTATTTTCTTCTAACTCATTTGCTAATACCAACTTCTAGTTAGAAATTGTGCCAATCAAGAGTTGATTGGAGTACCTTTGGTTTTACTTCTACGTACATCAAGCTAGTGTGTGGTTTTATGCATCTTAAGTAAAAACTGAAAGAAAAAACTAACTATGACTTATAGGAGCTTTACTAGGGGTTAACTAGTTATTGTAATTGCTGAATGAAGTATGATGCCTAAGTTTTTCATAGTAAATGAACCTACAACTCTAATGTGGTGTGTTTCCCTATAAATCTTTTTATAACCTCCCATGGGATAAACTATCAAAATATGTACGGAAAAACTTGAACTTTCATGGTGGCTAGAATTTTGAGTTTCCTTTAATGATATTATTTTTTGGCAGGGATACATAAATTAAGGACATGAGTGGAGAAAGTAAAATGATCGAAATGATGGAGGTATTCTCTTGATATTACATAAGTTGATGCTGTAATGTTGTACTGAAGTTGGACTGTATATAGTTCTTTCATCCATCCTCTCTATCCCACTTGGACAGTATTTATAGCTGATACGTATTCATGCTAACATTCTAGTTCATCATTTACAGTAACTGCCAAAGTATTCTCTATGTTTGTTTTCATAAGCTAATAACTGAGATAAATCATAAATGCTTGCTTAAATTGTGTTGACAGTTATCTCTAACATTGTGATTCAGCCATTCGAGTGGCTGCTCAGTGGTATCAAAATCATCTTGGTGGTGCTACTTGTGTTCTACTCATAACCAATGACAGAGAGAATAGGAGAAGGGCTATGGAAGAGGGAATTTGTGCAGAGACAAGTATGTTAGATGTTTATTTATTTTTAGCTTTGCTTCAAGCCTCTTGGTGTCTACCATGTGGCGTGCTTGTAATGATTATGTTCTCTCTCTTGCTCTCAAGCTTTTTATGTCATGCTGGTTAATTTGTTTGGTCTTTACAGTTGCATCTTATGTTAGATCATTGGGGCAAACTCATTTACTTGATCTGTTAGTGCAAAGTGCAAATACAGATGCAAATAAGGAGGATGTTGAAGATTTGAGGCCATCGAAGAGAAAAGTTCTTTACTCCGAGGTATGACAATGAATATGCTAAACTATATTTATTTCAGGAAAAATACTAGAGTCTTATAAGGTTTCTGGTTATTAACATATCCTACTCTTCTTTCTTTCAGCATAAACCCATGTCAGAAATTGCTTCTGGTTTGCATCGTGGTATATATCATCAAGGAAAGCGCCGGGTTAAGCGTTACAACCCATTTGAAGCATATGTTGGAAGTGAGAGTATTGGTGATGAAATTATTATTTATAGGCGTACCAACATGAACCGGGCTTTTGATGGTGATGTTGTGGCAGTTGAACTTCTATCTCGAGATCAGTGGCATGAAGAGAAGTCTCTGACTATAGCAGACGAAGGTAATTTTTTTTTTTATTCTCACGAATATTTTATTAATTGAAAACTGAGAAGGGTAGTGTGAATTATGTAAGGATCTAACATACAAATGTGTGTGTGGATGTTTGTGTAGGGTGAAGGGGGGATAAGGATTAGGTTAGAGTCATATTTTCATGAATTATGAGAATTCTAATTATATGATAACTTAAATATGAAAATATAGAAAGTTTAGCAAATTCCCTGAGTGAAAATGACTAAAGACTTTCCTTTGTGTTTCCAATATAAATTTCTATTCAGTATGGTTGGTGGCCTGGCTGGTGGGAGGGGGCAAAGGATTAGGTTGGGAGTTTGATTTTCATGAGTTATTACAATTTCAATTATATGCCAACTCCATGAAGATGGCAAAAAGGTTAACAACGTCCCTGTCAGGAAGTTACTAATTAATTTTCTCTGTATTTCTAATTCAAGCTTAAATTGTGCTTTGAATTTCACTCTCTCTCATTCTTTCTCACACACACACGGGCGGTTGTGCACACACACACAATCCCATTATGATGCACACATGATTGTATTATTGCAGAAGATGATGAGGAAGAAGATGTTCATCTTGTTCCTGGTAGGCTGATGATGCTCCAACAGCTGCATCTAGTGGAGATTCTTCTAGTTCACCACGCCCAGCTGGACGTGTCATTGGTATCAGAAAGCTTAACTGGCATTCGTAAGCTGTTGTGGCAATTTGTTAGATTCCTCTGTTCCATGGGGTGTCTTTTGCTGTACACATTATATGCTTTCTCTTATACAAATAAATACTTAAACATGTTCTAGATGTATATGTACCCATATATATTTTTGTGTATACAGTCTATAAGATCAAGATGACACTAACATTTGTAGTTAATGTTGAAAATATACTTCCACCTAGCTTTATAGTTTTTTTTTGGGGATTTTATAAATTTTGTCTTCTATATTACAGTTTTGTTTCTTTACTTTTTCAAGATGGTTGCACAGATTTTGTACCATGGTGGAGTTGTTCGTTTGATGTTTTGAAACTAATGTAGTTATTGTGGTTCCTTGAATCCAATATCTATGCCTGCTGGAAGTGGTGGCTTTGCCTCTGCTGTATTTGTTTCCAAAGATCATCAGATTCCTAAAATTAGAATCCAAACTCGTCAGCTTGAGAATCTTTTGGACAAGAGAATTATTGTAGTAGTTGATTCTTGGGACCGTCTTTCTAGATATCCTTCAGAACATTAG

mRNA sequence

ATGATATATATTGTGGGCTTCGCTTTGCAAAAATGTGAGTCTTCAGTTGCTTATTTGGGTGCTTCTGCATCGCCAATTCTAGTTCTCGACACCAATGTTGTTCTTACTCAGATTGGTTTGCTTGAGAATTCTGCTATTGATGACGTGGTTATGCTATCAGTTGTGCTGGATGAGGTTAAGAACAAGAACCTATCTGTTTACAACAGAGTTAGAGCTCTCTGCAGCAATCCCCTGTGGAGATTCTTTGTTTTCTTTAACGAGCACCACAATAACTGCCAAACCATTCGAGTGGCTGCTCAGTGGTATCAAAATCATCTTGGTGGTGCTACTTGTGTTCTACTCATAACCAATGACAGAGAGAATAGGAGAAGGGCTATGGAAGAGGGAATTTGTGCAGAGACAATTGCATCTTATGTTAGATCATTGGGGCAAACTCATTTACTTGATCTGTTAGTGCAAAGTGCAAATACAGATGCAAATAAGGAGGATGTTGAAGATTTGAGGCCATCGAAGAGAAAAGTTCTTTACTCCGAGCATAAACCCATGTCAGAAATTGCTTCTGGTTTGCATCGTGGTATATATCATCAAGGAAAGCGCCGGGTTAAGCGTTACAACCCATTTGAAGCATATGTTGGAAGTGAGAGTATTGGTGATGAAATTATTATTTATAGGCGTACCAACATGAACCGGGCTTTTGATGGTGATGTTGTGGCAGTTGAACTTCTATCTCGAGATCAGTGGCATGAAGAGAAGTCTCTGACTATAGCAGACGAAGAAGATGATGAGGAAGAAGATGTTCATCTTGTTCCTGGTAGGCTGATGATGCTCCAACAGCTGCATCTAGTGGAGATTCTTCTAGTTCACCACGCCCAGCTGGACGTGTCATTGGTATCAGAAAGCTTAACTGGCATTCGTAAGCTGTTGTGGCAATTTGTTAGATTCCTCTGTTCCATGGGGTGTCTTTTGCTTTATTGTGGTTCCTTGAATCCAATATCTATGCCTGCTGGAAGTGGTGGCTTTGCCTCTGCTGTATTTGTTTCCAAAGATCATCAGATTCCTAAAATTAGAATCCAAACTCGTCAGCTTGAGAATCTTTTGGACAAGAGAATTATTGTAGTAGTTGATTCTTGGGACCGTCTTTCTAGATATCCTTCAGAACATTAG

Coding sequence (CDS)

ATGATATATATTGTGGGCTTCGCTTTGCAAAAATGTGAGTCTTCAGTTGCTTATTTGGGTGCTTCTGCATCGCCAATTCTAGTTCTCGACACCAATGTTGTTCTTACTCAGATTGGTTTGCTTGAGAATTCTGCTATTGATGACGTGGTTATGCTATCAGTTGTGCTGGATGAGGTTAAGAACAAGAACCTATCTGTTTACAACAGAGTTAGAGCTCTCTGCAGCAATCCCCTGTGGAGATTCTTTGTTTTCTTTAACGAGCACCACAATAACTGCCAAACCATTCGAGTGGCTGCTCAGTGGTATCAAAATCATCTTGGTGGTGCTACTTGTGTTCTACTCATAACCAATGACAGAGAGAATAGGAGAAGGGCTATGGAAGAGGGAATTTGTGCAGAGACAATTGCATCTTATGTTAGATCATTGGGGCAAACTCATTTACTTGATCTGTTAGTGCAAAGTGCAAATACAGATGCAAATAAGGAGGATGTTGAAGATTTGAGGCCATCGAAGAGAAAAGTTCTTTACTCCGAGCATAAACCCATGTCAGAAATTGCTTCTGGTTTGCATCGTGGTATATATCATCAAGGAAAGCGCCGGGTTAAGCGTTACAACCCATTTGAAGCATATGTTGGAAGTGAGAGTATTGGTGATGAAATTATTATTTATAGGCGTACCAACATGAACCGGGCTTTTGATGGTGATGTTGTGGCAGTTGAACTTCTATCTCGAGATCAGTGGCATGAAGAGAAGTCTCTGACTATAGCAGACGAAGAAGATGATGAGGAAGAAGATGTTCATCTTGTTCCTGGTAGGCTGATGATGCTCCAACAGCTGCATCTAGTGGAGATTCTTCTAGTTCACCACGCCCAGCTGGACGTGTCATTGGTATCAGAAAGCTTAACTGGCATTCGTAAGCTGTTGTGGCAATTTGTTAGATTCCTCTGTTCCATGGGGTGTCTTTTGCTTTATTGTGGTTCCTTGAATCCAATATCTATGCCTGCTGGAAGTGGTGGCTTTGCCTCTGCTGTATTTGTTTCCAAAGATCATCAGATTCCTAAAATTAGAATCCAAACTCGTCAGCTTGAGAATCTTTTGGACAAGAGAATTATTGTAGTAGTTGATTCTTGGGACCGTCTTTCTAGATATCCTTCAGAACATTAG

Protein sequence

MIYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKNKNLSVYNRVRALCSNPLWRFFVFFNEHHNNCQTIRVAAQWYQNHLGGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVEDLRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRTNMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILLVHHAQLDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFVSKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH*

Homology

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BLAST of CSPI03G05580 vs. ExPASy Swiss-Prot
Match: Q9SHL7 (Exosome complex exonuclease RRP44 homolog A OS=Arabidopsis thaliana OX=3702 GN=RRP44A PE=2 SV=2)

HSP 1 Score: 434.5 bits (1116), Expect = 1.3e-120
Identity = 236/405 (58.27%), Postives = 284/405 (70.12%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY   F+ + C+SS A L  S+S I+V+DTNVVL QI LLEN AID VV+LSVVLDEVKN
Sbjct: 29  IYCGAFSCKSCDSSAARL--SSSKIIVVDTNVVLHQIDLLENKAIDTVVVLSVVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           +N SVYNR+R LCSNP  +F+VF N  H               +N + IRVA  WYQ HL
Sbjct: 89  RNRSVYNRIRLLCSNPARQFYVFSNHVHKDTYVQAMEKESANDHNDRAIRVATLWYQKHL 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           G  + VLL+TNDREN+R+A EEGI AETI +YV+SLGQ  LLDLL Q  N D   ED +D
Sbjct: 149 GDTSQVLLVTNDRENKRKATEEGISAETIEAYVKSLGQPELLDLLAQPTNEDITMEDADD 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
            RPSKRK++Y EHKPMSEI +GLHRGIYHQGK RV R+NP+EAYVGSESIG+EIIIY R+
Sbjct: 209 SRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIIIYGRS 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGD+VAVELL RDQW +EK+L+IA+E+D+E++ VHL P  +    +       L
Sbjct: 269 NMNRAFDGDIVAVELLPRDQWQDEKALSIAEEDDEEDDTVHLAPDNVDDAPRTS----NL 328

Query: 302 VHHAQLDVSLV----SESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFAS 361
            H    D +      S  + G+ +  W              YCGSL P+S+PAGSGG A 
Sbjct: 329 SHETSGDKNAAPVRPSGRVVGVIRRNWH------------SYCGSLEPMSLPAGSGGTAH 388

Query: 362 AVFVSKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           A+FVSKD +IPKIRI TRQL+NLLD RI+V VDSWDR SRYPS H
Sbjct: 389 ALFVSKDRRIPKIRINTRQLQNLLDMRIVVAVDSWDRQSRYPSGH 415

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BLAST of CSPI03G05580 vs. ExPASy Swiss-Prot
Match: Q9CSH3 (Exosome complex exonuclease RRP44 OS=Mus musculus OX=10090 GN=Dis3 PE=1 SV=4)

HSP 1 Score: 188.3 bits (477), Expect = 1.6e-46
Identity = 135/384 (35.16%), Postives = 195/384 (50.78%), Query Frame = 0


Query: 27  LVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKNKNLSVYNRVRALCSNPLWRFFVFFN 86
           L+ DTNV+L QI +LE+ AI +V++L  V+ EV+N++  +Y R+R + +N    F+ F N
Sbjct: 66  LLPDTNVLLHQIDVLEHPAIRNVIVLQTVMQEVRNRSAPIYKRIRDVTNNQEKHFYTFTN 125

Query: 87  EHH---------------NNCQTIRVAAQWYQNHLGGATC-----VLLITNDRENRRRAM 146
           EHH                N + IRVAA+WY  HL          V+LITNDR+N+ +A+
Sbjct: 126 EHHKETYIEQEQGENANDRNDRAIRVAAKWYNEHLKRVAADSQLQVILITNDRKNKEKAV 185

Query: 147 EEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVEDLRPSKRKVLYSEHKPMSEIA 206
           +EGI A T   YV+SL  T   +L+ + A       ++E       K+++SEH P+S++ 
Sbjct: 186 QEGIPAFTCEEYVKSL--TANPELIDRLAYLSDEMNEIE-----SGKIIFSEHLPLSKLQ 245

Query: 207 SGLHRGIYHQGKRRVKRYNPFEAYV---GSESIGDEIIIYRRTNMNRAFDGDVVAVELLS 266
            G+  G Y QG  R  R N  EA V   G +    EI+I    ++NRA   D+VAVELL 
Sbjct: 246 QGIKSGSYLQGTFRASRENYLEATVWIHGDKEEEKEILIQGIKHLNRAVHEDIVAVELLP 305

Query: 267 RDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILLVHHAQLDVSLVSESLTG 326
           R QW    S+ + D+E   E+DV     R          E+LL       +   +  + G
Sbjct: 306 RSQWVAPSSV-VLDDEGQNEDDVEKDEER----------ELLLKTAVSEKMLRPTGRVVG 365

Query: 327 IRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFVSKDHQIPKIRIQTRQLE 386
           I K  W+             YCG L+   +          +F   D +IP+IRI+TRQ  
Sbjct: 366 IIKRNWR------------PYCGMLSKSDIKES----RRHLFTPADKRIPRIRIETRQAS 415

Query: 387 NLLDKRIIVVVDSWDRLSRYPSEH 388
            L  +RIIV +D W R SRYP+ H
Sbjct: 426 ALEGRRIIVAIDGWPRNSRYPNGH 415

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BLAST of CSPI03G05580 vs. ExPASy Swiss-Prot
Match: Q9Y2L1 (Exosome complex exonuclease RRP44 OS=Homo sapiens OX=9606 GN=DIS3 PE=1 SV=2)

HSP 1 Score: 186.4 bits (472), Expect = 6.0e-46
Identity = 133/384 (34.64%), Postives = 193/384 (50.26%), Query Frame = 0


Query: 27  LVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKNKNLSVYNRVRALCSNPLWRFFVFFN 86
           L+ DTNV+L QI +LE+ AI +V++L  VL EV+N++  VY R+R + +N    F+ F N
Sbjct: 66  LLPDTNVLLHQIDVLEDPAIRNVIVLQTVLQEVRNRSAPVYKRIRDVTNNQEKHFYTFTN 125

Query: 87  EHH---------------NNCQTIRVAAQWYQNHLGGATC-----VLLITNDRENRRRAM 146
           EHH                N + IRVAA+WY  HL   +      V+ ITNDR N+ +A+
Sbjct: 126 EHHRETYVEQEQGENANDRNDRAIRVAAKWYNEHLKKMSADNQLQVIFITNDRRNKEKAI 185

Query: 147 EEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVEDLRPSKRKVLYSEHKPMSEIA 206
           EEGI A T   YV+SL  T   +L+ + A       ++E       K+++SEH P+S++ 
Sbjct: 186 EEGIPAFTCEEYVKSL--TANPELIDRLACLSEEGNEIE-----SGKIIFSEHLPLSKLQ 245

Query: 207 SGLHRGIYHQGKRRVKRYNPFEAYV---GSESIGDEIIIYRRTNMNRAFDGDVVAVELLS 266
            G+  G Y QG  R  R N  EA V   G      EII+    ++NRA   D+VAVELL 
Sbjct: 246 QGIKSGTYLQGTFRASRENYLEATVWIHGDNEENKEIILQGLKHLNRAVHEDIVAVELLP 305

Query: 267 RDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILLVHHAQLDVSLVSESLTG 326
           + QW    S+ + DE  +EE+           +++    E +L       +   +  + G
Sbjct: 306 KSQWVAPSSVVLHDEGQNEED-----------VEKEEETERMLKTAVSEKMLKPTGRVVG 365

Query: 327 IRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFVSKDHQIPKIRIQTRQLE 386
           I K  W+             YCG L+   +          +F   D +IP+IRI+TRQ  
Sbjct: 366 IIKRNWR------------PYCGMLSKSDIKES----RRHLFTPADKRIPRIRIETRQAS 415

Query: 387 NLLDKRIIVVVDSWDRLSRYPSEH 388
            L  +RIIV +D W R SRYP+ H
Sbjct: 426 TLEGRRIIVAIDGWPRNSRYPNGH 415

[Close]

BLAST of CSPI03G05580 vs. ExPASy Swiss-Prot
Match: P37202 (Exosome complex exonuclease dis3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dis3 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.4e-39
Identity = 118/380 (31.05%), Postives = 189/380 (49.74%), Query Frame = 0


Query: 27  LVLDTNVVLTQIGLLEN-SAIDDVVMLSVVLDEVKNKNLSVYNRVRALCSNPLWRFFVFF 86
           L+ D+N+    I  LE+ +   DV++L  V  E+ +K++ +YNR++ LC     RF  F 
Sbjct: 85  LIPDSNIFYHCIDALEHPNNFFDVIILQTVFSEISSKSIPLYNRMKRLCQEKTKRFTPFS 144

Query: 87  NE---------------HHNNCQTIRVAAQWYQNHLGG-ATCVLLITNDRENRRRAMEEG 146
           NE               +  N + IR AA W+ +HL      ++L+T+DREN R A E+G
Sbjct: 145 NEFFVDTFVERLDDESANDRNDRAIRNAASWFASHLASLGIKIVLLTDDRENARLAAEQG 204

Query: 147 ICAETIASYVRSLGQTH-LLDLLVQSANTDANKEDVEDLRPSKRKVLYSEHKPMSEIASG 206
           I   T+  YV+ L  +  LLD++   A+  A+KE VE    S  K +Y  H  MS + + 
Sbjct: 205 IQVSTLKDYVQYLPDSEILLDMVSAIADAIASKEQVE----SGTKNVYELHWSMSRLLAC 264

Query: 207 LHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRTNMNRAFDGDVVAVELLSRDQWH 266
           +  G  H+G   +  YN  E  V        +++  R N+NRA  GD+V +++L +DQW 
Sbjct: 265 IKNGEVHKGLINISTYNYLEGSVVVPGYNKPVLVSGRENLNRAVQGDIVCIQILPQDQWK 324

Query: 267 EEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILLVHHAQLDVSLVSESLTGIRKLL 326
            E     A+E  D++EDV +         +++ +E++   +A     +V     GI K  
Sbjct: 325 TE-----AEEIADDDEDVVVSTAAEPDSARINDLELITKRNAHPTAKVV-----GILKRN 384

Query: 327 WQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFVS-KDHQIPKIRIQTRQLENLLD 386
           W+             Y G ++  ++    GG    V ++  D ++PKIR +TRQ   L+ 
Sbjct: 385 WR------------PYVGHVDNATIAQSKGGSQQTVLLTPMDRRVPKIRFRTRQAPRLVG 438

Query: 387 KRIIVVVDSWDRLSRYPSEH 388
           +RI+V +D WD  SRYP  H
Sbjct: 445 RRIVVAIDLWDASSRYPEGH 438

[Close]

BLAST of CSPI03G05580 vs. ExPASy Swiss-Prot
Match: Q08162 (Exosome complex exonuclease DIS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DIS3 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.5e-36
Identity = 118/396 (29.80%), Postives = 184/396 (46.46%), Query Frame = 0


Query: 27  LVLDTNVVLTQIGLLEN-SAIDDVVMLSVVLDEVKNKNLSVYNRVRALC--SNPLWRFFV 86
           +VLDTNVVL  I LLEN +   DV++  +VLDEV+NK+  VY R+R LC  S+   RF V
Sbjct: 88  VVLDTNVVLQAIDLLENPNCFFDVIVPQIVLDEVRNKSYPVYTRLRTLCRDSDDHKRFIV 147

Query: 87  FFNE---------------HHNNCQTIRVAAQWYQNHLGGATC-VLLITNDRENRRRA-- 146
           F NE               +  N + IR   QWY  HL      V+L+TNDR NR  A  
Sbjct: 148 FHNEFSEHTFVERLPNETINDRNDRAIRKTCQWYSEHLKPYDINVVLVTNDRLNREAATK 207

Query: 147 -MEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVEDLRPSKRKVLYSEHKPMSE 206
            +E  I  +++  Y+  L      D+       D+  +D+E  R +     + E+   + 
Sbjct: 208 EVESNIITKSLVQYIELLPNAD--DIRDSIPQMDSFDKDLE--RDTFSDFTFPEYYSTAR 267

Query: 207 IASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRTNMNRAFDGDVVAVELLSR 266
           +  GL  G+ +QG  ++  YN  E  V        ++I  + N+NRAF+GD V VELL +
Sbjct: 268 VMGGLKNGVLYQGNIQISEYNFLEGSVSLPRFSKPVLIVGQKNLNRAFNGDQVIVELLPQ 327

Query: 267 DQWHEEKSLTIADE-------------EDDEEEDVHLVPGRLMMLQQLHLVEILLVHHAQ 326
            +W    S+ +  E             +DD+  +       +   Q+  L +  ++    
Sbjct: 328 SEWKAPSSIVLDSEHFDVNDNPDIEAGDDDDNNESSSNTTVISDKQRRLLAKDAMIAQRS 387

Query: 327 LDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFVSKDHQ 386
             +   ++ +  I++  W+             Y G L P S+   S    +   +  D  
Sbjct: 388 KKIQPTAK-VVYIQRRSWR------------QYVGQLAPSSVDPQSSSTQNVFVILMDKC 447

Query: 387 IPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           +PK+RI+TR+   LLDKRI++ +DSW    +YP  H
Sbjct: 448 LPKVRIRTRRAAELLDKRIVISIDSWPTTHKYPLGH 466

[Close]

BLAST of CSPI03G05580 vs. ExPASy TrEMBL
Match: A0A0A0K821 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G074840 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 5.0e-152
Identity = 300/401 (74.81%), Postives = 315/401 (78.55%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY      +KC+SSVA LGAS SPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN
Sbjct: 29  IYCGASICKKCDSSVARLGASTSPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           KNLSVYNRVRALCSNPL RFFVF NEHH                N + IRVAAQWYQNHL
Sbjct: 89  KNLSVYNRVRALCSNPLRRFFVFSNEHHKDTYIKDMSGESKNDRNDRAIRVAAQWYQNHL 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           GGAT VLLITNDRENRR+A+EEGI AETI SYV+SLGQTHLLDLLVQSAN DAN EDVED
Sbjct: 149 GGATRVLLITNDRENRRKAIEEGIGAETIESYVKSLGQTHLLDLLVQSANEDANMEDVED 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
           LRPSKRKVLYSEHKPMSEI SGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIY RT
Sbjct: 209 LRPSKRKVLYSEHKPMSEITSGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYGRT 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGDVVAVELLSRDQWHEEKSLTIAD+EDDEEEDVHLVPG      +   V    
Sbjct: 269 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADDEDDEEEDVHLVPGSADDAPRAASVLQGS 328

Query: 302 VHHAQLDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFV 361
              +       +  + GI K  W              YCGSL+P+ MPAGSGG A A+FV
Sbjct: 329 SGDSSSSSPRPAGRVIGIIKRNWH------------SYCGSLDPMPMPAGSGGIAYALFV 388

Query: 362 SKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           SKD +IPKIRIQTRQLENLLDKRIIV VDSWDRLSRYPS H
Sbjct: 389 SKDRRIPKIRIQTRQLENLLDKRIIVAVDSWDRLSRYPSGH 417

[Close]

BLAST of CSPI03G05580 vs. ExPASy TrEMBL
Match: A0A0A0L2I2 (PINc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G089000 PE=4 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 1.1e-151
Identity = 299/384 (77.86%), Postives = 309/384 (80.47%), Query Frame = 0


Query: 14  SSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKNKNLSVYNRVRAL 73
           SSVA LGAS SPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKNKNLSVYNRVRAL
Sbjct: 19  SSVACLGASTSPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKNKNLSVYNRVRAL 78

Query: 74  CSNPLWRFFVFFNEHHNNCQTIRVAA--------QWYQNHLGGATCVLLITNDRENRRRA 133
           CSNPLWRFFVFFNEHH +     ++         +WYQNHLGGATCVLLITNDRENRRRA
Sbjct: 79  CSNPLWRFFVFFNEHHKDTYIKDMSGESKMIEMMEWYQNHLGGATCVLLITNDRENRRRA 138

Query: 134 MEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVEDLRPSKRKVLYSEHKPMSEI 193
           MEEGICAETI SYVRSLGQTHLLDLLVQSAN DAN EDVEDLRPSKRKVLYSEHKPMSEI
Sbjct: 139 MEEGICAETIESYVRSLGQTHLLDLLVQSANEDANMEDVEDLRPSKRKVLYSEHKPMSEI 198

Query: 194 ASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRTNMNRAFDGDVVAVELLSRD 253
           ASGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIYRRTNMNRAFDGDVVAVELLSRD
Sbjct: 199 ASGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYRRTNMNRAFDGDVVAVELLSRD 258

Query: 254 QWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILLVHHAQLDVSLVSES--LTG 313
           QWHEEKSLTIA           L  GR     +L   +      +    S    +  + G
Sbjct: 259 QWHEEKSLTIASMVGG------LAGGRGQRRIRLGADDAPTAASSGDSSSSPRPAGRVIG 318

Query: 314 IRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFVSKDHQIPKIRIQTRQLE 373
           IRKL W              YCGSLNP+SMPAGSGGFASAVFVSKDHQIPKIRIQTRQLE
Sbjct: 319 IRKLNWH------------SYCGSLNPMSMPAGSGGFASAVFVSKDHQIPKIRIQTRQLE 378

Query: 374 NLLDKRIIVVVDSWDRLSRYPSEH 388
           NLLDKRIIVVVDSWDRLSRYPSEH
Sbjct: 379 NLLDKRIIVVVDSWDRLSRYPSEH 384

[Close]

BLAST of CSPI03G05580 vs. ExPASy TrEMBL
Match: A0A5A7SKL0 (Exosome complex exonuclease RRP44-like protein A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00330 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 7.2e-151
Identity = 298/401 (74.31%), Postives = 312/401 (77.81%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY      +KC+SSVA LG+S SPILVLDTNVVLTQI LLEN AIDDVVMLSVVLDEVKN
Sbjct: 29  IYCGASICKKCDSSVARLGSSTSPILVLDTNVVLTQIDLLENPAIDDVVMLSVVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           KNLSVYNRVRALCSNPL RFFVF NEHH                N + IRVAA WYQNHL
Sbjct: 89  KNLSVYNRVRALCSNPLRRFFVFSNEHHKDTYIKDMSGESKNDRNDRAIRVAALWYQNHL 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           GGAT VLLITNDRENRR+A+EEGICAETI SYVRSLGQTHLLDLLVQSAN DAN EDVED
Sbjct: 149 GGATRVLLITNDRENRRKAIEEGICAETIESYVRSLGQTHLLDLLVQSANEDANMEDVED 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
           LRPSKRKVLYSEHKPMSEI SGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIY RT
Sbjct: 209 LRPSKRKVLYSEHKPMSEITSGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYGRT 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPG      +        
Sbjct: 269 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGSSDDAPRAASALQGS 328

Query: 302 VHHAQLDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFV 361
              +       +  + GI K  W              YCGSL+P+ MPAGSGG A A+FV
Sbjct: 329 SGDSSSSSPRPAGRVIGIIKRNWH------------SYCGSLDPMPMPAGSGGVAYALFV 388

Query: 362 SKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           SKD +IPKIRIQTRQLENLLDKRIIV VDSWDRLSRYPS H
Sbjct: 389 SKDRRIPKIRIQTRQLENLLDKRIIVAVDSWDRLSRYPSGH 417

[Close]

BLAST of CSPI03G05580 vs. ExPASy TrEMBL
Match: A0A1S3BZS3 (exosome complex exonuclease RRP44 homolog A OS=Cucumis melo OX=3656 GN=LOC103495216 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 7.2e-151
Identity = 298/401 (74.31%), Postives = 312/401 (77.81%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY      +KC+SSVA LG+S SPILVLDTNVVLTQI LLEN AIDDVVMLSVVLDEVKN
Sbjct: 29  IYCGASICKKCDSSVARLGSSTSPILVLDTNVVLTQIDLLENPAIDDVVMLSVVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           KNLSVYNRVRALCSNPL RFFVF NEHH                N + IRVAA WYQNHL
Sbjct: 89  KNLSVYNRVRALCSNPLRRFFVFSNEHHKDTYIKDMSGESKNDRNDRAIRVAALWYQNHL 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           GGAT VLLITNDRENRR+A+EEGICAETI SYVRSLGQTHLLDLLVQSAN DAN EDVED
Sbjct: 149 GGATRVLLITNDRENRRKAIEEGICAETIESYVRSLGQTHLLDLLVQSANEDANMEDVED 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
           LRPSKRKVLYSEHKPMSEI SGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIY RT
Sbjct: 209 LRPSKRKVLYSEHKPMSEITSGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYGRT 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPG      +        
Sbjct: 269 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGSSDDAPRAASALQGS 328

Query: 302 VHHAQLDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFV 361
              +       +  + GI K  W              YCGSL+P+ MPAGSGG A A+FV
Sbjct: 329 SGDSSSSSPRPAGRVIGIIKRNWH------------SYCGSLDPMPMPAGSGGVAYALFV 388

Query: 362 SKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           SKD +IPKIRIQTRQLENLLDKRIIV VDSWDRLSRYPS H
Sbjct: 389 SKDRRIPKIRIQTRQLENLLDKRIIVAVDSWDRLSRYPSGH 417

[Close]

BLAST of CSPI03G05580 vs. ExPASy TrEMBL
Match: A0A6J1I5P7 (exosome complex exonuclease RRP44 homolog A-like OS=Cucurbita maxima OX=3661 GN=LOC111470918 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.9e-148
Identity = 293/401 (73.07%), Postives = 312/401 (77.81%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY      +KC+SSVA L AS SPILVLDTNVVL QI LLEN AIDDVVMLS+VLDEVKN
Sbjct: 29  IYCGTSICKKCDSSVARLSASTSPILVLDTNVVLNQIDLLENPAIDDVVMLSIVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           KNLSVYNRVRALCSNPL RFFVF NEHH                N + IRVAAQWYQNH+
Sbjct: 89  KNLSVYNRVRALCSNPLRRFFVFSNEHHKDTYVKEMSGESKNDRNDRAIRVAAQWYQNHI 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           GGAT VLLITNDRENRR+A+EEGICAETI SYVRSLGQTHLLDLLVQSAN DAN E+VED
Sbjct: 149 GGATRVLLITNDRENRRKAVEEGICAETIESYVRSLGQTHLLDLLVQSANEDANMEEVED 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
           +RPSKRKVLY EHKPMSEI SGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIY RT
Sbjct: 209 MRPSKRKVLYFEHKPMSEITSGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYGRT 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGDVVAVELLSRDQWHEEKSLTIADE+DDEEEDVHLVPG      +   V +  
Sbjct: 269 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEDDDEEEDVHLVPGSADDAPRTTSV-VGS 328

Query: 302 VHHAQLDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFV 361
              +       +  + GI K  W              YCGSL+P+ MPAGSGG A A+FV
Sbjct: 329 NGDSTSSSPRPAGRVIGIIKRNWH------------SYCGSLDPMPMPAGSGGVAYALFV 388

Query: 362 SKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           SKD +IPKIRIQTRQLENLLDKRIIV VDSWDRLSRYPS H
Sbjct: 389 SKDRRIPKIRIQTRQLENLLDKRIIVAVDSWDRLSRYPSGH 416

[Close]

BLAST of CSPI03G05580 vs. NCBI nr
Match: KAE8650154.1 (hypothetical protein Csa_010466 [Cucumis sativus])

HSP 1 Score: 609.8 bits (1571), Expect = 1.7e-170
Identity = 315/331 (95.17%), Postives = 317/331 (95.77%), Query Frame = 0


Query: 1   MIYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVK 60
           MIYIVGFALQKCESSVA LGAS SPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVK
Sbjct: 1   MIYIVGFALQKCESSVACLGASTSPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVK 60

Query: 61  NKNLSVYNRVRALCSNPLWRFFVFFNEHHNNC-------QTIRVAAQWYQNHLGGATCVL 120
           NKNLSVYNRVRALCSNPLWRFFVFFNEHH +        +TIRVAAQWYQNHLGGATCVL
Sbjct: 61  NKNLSVYNRVRALCSNPLWRFFVFFNEHHKDTYIKDMSGETIRVAAQWYQNHLGGATCVL 120

Query: 121 LITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVEDLRPSKRK 180
           LITNDRENRRRAMEEGICAETI SYVRSLGQTHLLDLLVQSANTDANKEDVEDLRPSKRK
Sbjct: 121 LITNDRENRRRAMEEGICAETIESYVRSLGQTHLLDLLVQSANTDANKEDVEDLRPSKRK 180

Query: 181 VLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRTNMNRAFD 240
           VLYSEHKPMSEIASGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIYRRTNMNRAFD
Sbjct: 181 VLYSEHKPMSEIASGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYRRTNMNRAFD 240

Query: 241 GDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILLVHHAQLD 300
           GDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILLVHHAQLD
Sbjct: 241 GDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILLVHHAQLD 300

Query: 301 VSLVSESLTGIRKLLWQFVRFLCSMGCLLLY 325
           VSLVSESLTGIRKLLWQFVRFLCSMGCLLLY
Sbjct: 301 VSLVSESLTGIRKLLWQFVRFLCSMGCLLLY 331

[Close]

BLAST of CSPI03G05580 vs. NCBI nr
Match: XP_008454914.1 (PREDICTED: exosome complex exonuclease RRP44 homolog A [Cucumis melo] >KAA0031280.1 exosome complex exonuclease RRP44-like protein A [Cucumis melo var. makuwa] >TYK06732.1 exosome complex exonuclease RRP44-like protein A [Cucumis melo var. makuwa])

HSP 1 Score: 543.5 bits (1399), Expect = 1.5e-150
Identity = 298/401 (74.31%), Postives = 312/401 (77.81%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY      +KC+SSVA LG+S SPILVLDTNVVLTQI LLEN AIDDVVMLSVVLDEVKN
Sbjct: 29  IYCGASICKKCDSSVARLGSSTSPILVLDTNVVLTQIDLLENPAIDDVVMLSVVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           KNLSVYNRVRALCSNPL RFFVF NEHH                N + IRVAA WYQNHL
Sbjct: 89  KNLSVYNRVRALCSNPLRRFFVFSNEHHKDTYIKDMSGESKNDRNDRAIRVAALWYQNHL 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           GGAT VLLITNDRENRR+A+EEGICAETI SYVRSLGQTHLLDLLVQSAN DAN EDVED
Sbjct: 149 GGATRVLLITNDRENRRKAIEEGICAETIESYVRSLGQTHLLDLLVQSANEDANMEDVED 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
           LRPSKRKVLYSEHKPMSEI SGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIY RT
Sbjct: 209 LRPSKRKVLYSEHKPMSEITSGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYGRT 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPG      +        
Sbjct: 269 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGSSDDAPRAASALQGS 328

Query: 302 VHHAQLDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFV 361
              +       +  + GI K  W              YCGSL+P+ MPAGSGG A A+FV
Sbjct: 329 SGDSSSSSPRPAGRVIGIIKRNWH------------SYCGSLDPMPMPAGSGGVAYALFV 388

Query: 362 SKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           SKD +IPKIRIQTRQLENLLDKRIIV VDSWDRLSRYPS H
Sbjct: 389 SKDRRIPKIRIQTRQLENLLDKRIIVAVDSWDRLSRYPSGH 417

[Close]

BLAST of CSPI03G05580 vs. NCBI nr
Match: XP_011658916.2 (exosome complex exonuclease RRP44 homolog A [Cucumis sativus] >KAE8645869.1 hypothetical protein Csa_017207 [Cucumis sativus])

HSP 1 Score: 542.7 bits (1397), Expect = 2.5e-150
Identity = 298/401 (74.31%), Postives = 313/401 (78.05%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY      +KC+SSVA LGAS SPILVLDTNVVLTQI LLEN AIDDVVMLSVVLDEVKN
Sbjct: 29  IYCGASICKKCDSSVARLGASTSPILVLDTNVVLTQIDLLENPAIDDVVMLSVVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           KNLSVYNRVRALCSNPL RFFVF NEHH                N + IRVAAQWYQNHL
Sbjct: 89  KNLSVYNRVRALCSNPLRRFFVFSNEHHKDTYIKDMSGESKNDRNDRAIRVAAQWYQNHL 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           GGAT VLLITNDRENRR+A+EEGI AETI SYV+SLGQTHLLDLLVQSAN DAN EDVED
Sbjct: 149 GGATRVLLITNDRENRRKAIEEGIGAETIESYVKSLGQTHLLDLLVQSANEDANMEDVED 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
           LRPSKRKVLYSEHKPMSEI SGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIY RT
Sbjct: 209 LRPSKRKVLYSEHKPMSEITSGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYGRT 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGDVVAVELLSRDQWHEEKSLTIAD+EDDEEEDVHLVPG      +   V    
Sbjct: 269 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADDEDDEEEDVHLVPGSADDAPRAASVLQGS 328

Query: 302 VHHAQLDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFV 361
              +       +  + GI K  W              YCGSL+P+ MPAGSGG A A+FV
Sbjct: 329 SGDSSSSSPRPAGRVIGIIKRNWH------------SYCGSLDPMPMPAGSGGIAYALFV 388

Query: 362 SKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           SKD +IPKIRIQTRQLENLLDKRIIV VDSWDRLSRYPS H
Sbjct: 389 SKDRRIPKIRIQTRQLENLLDKRIIVAVDSWDRLSRYPSGH 417

[Close]

BLAST of CSPI03G05580 vs. NCBI nr
Match: XP_038887941.1 (exosome complex exonuclease RRP44 homolog A [Benincasa hispida])

HSP 1 Score: 536.6 bits (1381), Expect = 1.8e-148
Identity = 296/401 (73.82%), Postives = 312/401 (77.81%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY      +KC+SSVA L A+ SPILVLDTNVVLTQI LLEN AIDDVVMLSVVLDEVKN
Sbjct: 29  IYCGTSICKKCDSSVARLSAT-SPILVLDTNVVLTQIDLLENPAIDDVVMLSVVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           KNLSVYNRVRALCSNPL RFFVF NEHH                N + IRVAAQWYQNHL
Sbjct: 89  KNLSVYNRVRALCSNPLRRFFVFSNEHHRDTYIKEMSGESKNDRNDRAIRVAAQWYQNHL 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           GGAT VLLI+NDRENRR+A+EEGICAETI SYVRSLGQTHLLDLLVQSAN DAN EDVED
Sbjct: 149 GGATRVLLISNDRENRRKAIEEGICAETIESYVRSLGQTHLLDLLVQSANEDANMEDVED 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
           LRPSKRKVLYSEHKPMSEI SGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIY RT
Sbjct: 209 LRPSKRKVLYSEHKPMSEITSGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYGRT 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGDVVAVELLSRDQWHEEKSLTI DEED+EEEDVHLVPG      +   V    
Sbjct: 269 NMNRAFDGDVVAVELLSRDQWHEEKSLTIVDEEDEEEEDVHLVPGSADDAPRAASVLQGS 328

Query: 302 VHHAQLDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFV 361
              +       +  + GI K  W              YCGSL+P+ MPAGSGG A A+FV
Sbjct: 329 SGDSSSSSPRPAGRVVGIIKRNWH------------SYCGSLDPMPMPAGSGGVAYALFV 388

Query: 362 SKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           SKD +IPKIRIQTRQLENLLDKRIIV VDSWDRLSRYPS H
Sbjct: 389 SKDRRIPKIRIQTRQLENLLDKRIIVAVDSWDRLSRYPSGH 416

[Close]

BLAST of CSPI03G05580 vs. NCBI nr
Match: XP_022972336.1 (exosome complex exonuclease RRP44 homolog A-like [Cucurbita maxima])

HSP 1 Score: 535.4 bits (1378), Expect = 4.0e-148
Identity = 293/401 (73.07%), Postives = 312/401 (77.81%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY      +KC+SSVA L AS SPILVLDTNVVL QI LLEN AIDDVVMLS+VLDEVKN
Sbjct: 29  IYCGTSICKKCDSSVARLSASTSPILVLDTNVVLNQIDLLENPAIDDVVMLSIVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           KNLSVYNRVRALCSNPL RFFVF NEHH                N + IRVAAQWYQNH+
Sbjct: 89  KNLSVYNRVRALCSNPLRRFFVFSNEHHKDTYVKEMSGESKNDRNDRAIRVAAQWYQNHI 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           GGAT VLLITNDRENRR+A+EEGICAETI SYVRSLGQTHLLDLLVQSAN DAN E+VED
Sbjct: 149 GGATRVLLITNDRENRRKAVEEGICAETIESYVRSLGQTHLLDLLVQSANEDANMEEVED 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
           +RPSKRKVLY EHKPMSEI SGLHRGIYHQGK RV RYNPFEAYVGSESIGDEIIIY RT
Sbjct: 209 MRPSKRKVLYFEHKPMSEITSGLHRGIYHQGKLRVNRYNPFEAYVGSESIGDEIIIYGRT 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGDVVAVELLSRDQWHEEKSLTIADE+DDEEEDVHLVPG      +   V +  
Sbjct: 269 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEDDDEEEDVHLVPGSADDAPRTTSV-VGS 328

Query: 302 VHHAQLDVSLVSESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFASAVFV 361
              +       +  + GI K  W              YCGSL+P+ MPAGSGG A A+FV
Sbjct: 329 NGDSTSSSPRPAGRVIGIIKRNWH------------SYCGSLDPMPMPAGSGGVAYALFV 388

Query: 362 SKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           SKD +IPKIRIQTRQLENLLDKRIIV VDSWDRLSRYPS H
Sbjct: 389 SKDRRIPKIRIQTRQLENLLDKRIIVAVDSWDRLSRYPSGH 416

[Close]

BLAST of CSPI03G05580 vs. TAIR 10
Match: AT2G17510.1 (ribonuclease II family protein )

HSP 1 Score: 434.5 bits (1116), Expect = 9.0e-122
Identity = 236/405 (58.27%), Postives = 284/405 (70.12%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY   F+ + C+SS A L  S+S I+V+DTNVVL QI LLEN AID VV+LSVVLDEVKN
Sbjct: 29  IYCGAFSCKSCDSSAARL--SSSKIIVVDTNVVLHQIDLLENKAIDTVVVLSVVLDEVKN 88

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           +N SVYNR+R LCSNP  +F+VF N  H               +N + IRVA  WYQ HL
Sbjct: 89  RNRSVYNRIRLLCSNPARQFYVFSNHVHKDTYVQAMEKESANDHNDRAIRVATLWYQKHL 148

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           G  + VLL+TNDREN+R+A EEGI AETI +YV+SLGQ  LLDLL Q  N D   ED +D
Sbjct: 149 GDTSQVLLVTNDRENKRKATEEGISAETIEAYVKSLGQPELLDLLAQPTNEDITMEDADD 208

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
            RPSKRK++Y EHKPMSEI +GLHRGIYHQGK RV R+NP+EAYVGSESIG+EIIIY R+
Sbjct: 209 SRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIIIYGRS 268

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGD+VAVELL RDQW +EK+L+IA+E+D+E++ VHL P  +    +       L
Sbjct: 269 NMNRAFDGDIVAVELLPRDQWQDEKALSIAEEDDEEDDTVHLAPDNVDDAPRTS----NL 328

Query: 302 VHHAQLDVSLV----SESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFAS 361
            H    D +      S  + G+ +  W              YCGSL P+S+PAGSGG A 
Sbjct: 329 SHETSGDKNAAPVRPSGRVVGVIRRNWH------------SYCGSLEPMSLPAGSGGTAH 388

Query: 362 AVFVSKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           A+FVSKD +IPKIRI TRQL+NLLD RI+V VDSWDR SRYPS H
Sbjct: 389 ALFVSKDRRIPKIRINTRQLQNLLDMRIVVAVDSWDRQSRYPSGH 415

[Close]

BLAST of CSPI03G05580 vs. TAIR 10
Match: AT2G17510.2 (ribonuclease II family protein )

HSP 1 Score: 434.5 bits (1116), Expect = 9.0e-122
Identity = 236/405 (58.27%), Postives = 284/405 (70.12%), Query Frame = 0


Query: 2   IYIVGFALQKCESSVAYLGASASPILVLDTNVVLTQIGLLENSAIDDVVMLSVVLDEVKN 61
           IY   F+ + C+SS A L  S+S I+V+DTNVVL QI LLEN AID VV+LSVVLDEVKN
Sbjct: 74  IYCGAFSCKSCDSSAARL--SSSKIIVVDTNVVLHQIDLLENKAIDTVVVLSVVLDEVKN 133

Query: 62  KNLSVYNRVRALCSNPLWRFFVFFNEHH---------------NNCQTIRVAAQWYQNHL 121
           +N SVYNR+R LCSNP  +F+VF N  H               +N + IRVA  WYQ HL
Sbjct: 134 RNRSVYNRIRLLCSNPARQFYVFSNHVHKDTYVQAMEKESANDHNDRAIRVATLWYQKHL 193

Query: 122 GGATCVLLITNDRENRRRAMEEGICAETIASYVRSLGQTHLLDLLVQSANTDANKEDVED 181
           G  + VLL+TNDREN+R+A EEGI AETI +YV+SLGQ  LLDLL Q  N D   ED +D
Sbjct: 194 GDTSQVLLVTNDRENKRKATEEGISAETIEAYVKSLGQPELLDLLAQPTNEDITMEDADD 253

Query: 182 LRPSKRKVLYSEHKPMSEIASGLHRGIYHQGKRRVKRYNPFEAYVGSESIGDEIIIYRRT 241
            RPSKRK++Y EHKPMSEI +GLHRGIYHQGK RV R+NP+EAYVGSESIG+EIIIY R+
Sbjct: 254 SRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIIIYGRS 313

Query: 242 NMNRAFDGDVVAVELLSRDQWHEEKSLTIADEEDDEEEDVHLVPGRLMMLQQLHLVEILL 301
           NMNRAFDGD+VAVELL RDQW +EK+L+IA+E+D+E++ VHL P  +    +       L
Sbjct: 314 NMNRAFDGDIVAVELLPRDQWQDEKALSIAEEDDEEDDTVHLAPDNVDDAPRTS----NL 373

Query: 302 VHHAQLDVSLV----SESLTGIRKLLWQFVRFLCSMGCLLLYCGSLNPISMPAGSGGFAS 361
            H    D +      S  + G+ +  W              YCGSL P+S+PAGSGG A 
Sbjct: 374 SHETSGDKNAAPVRPSGRVVGVIRRNWH------------SYCGSLEPMSLPAGSGGTAH 433

Query: 362 AVFVSKDHQIPKIRIQTRQLENLLDKRIIVVVDSWDRLSRYPSEH 388
           A+FVSKD +IPKIRI TRQL+NLLD RI+V VDSWDR SRYPS H
Sbjct: 434 ALFVSKDRRIPKIRINTRQLQNLLDMRIVVAVDSWDRQSRYPSGH 460

The following BLAST results are available for this feature:

BLAST of CSPI03G05580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SHL7	1.3e-120	58.27	Exosome complex exonuclease RRP44 homolog A OS=Arabidopsis thaliana OX=3702 GN=R...	[more]
Q9CSH3	1.6e-46	35.16	Exosome complex exonuclease RRP44 OS=Mus musculus OX=10090 GN=Dis3 PE=1 SV=4	[more]
Q9Y2L1	6.0e-46	34.64	Exosome complex exonuclease RRP44 OS=Homo sapiens OX=9606 GN=DIS3 PE=1 SV=2	[more]
P37202	1.4e-39	31.05	Exosome complex exonuclease dis3 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]
Q08162	1.5e-36	29.80	Exosome complex exonuclease DIS3 OS=Saccharomyces cerevisiae (strain ATCC 204508...	[more]

BLAST of CSPI03G05580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K821	5.0e-152	74.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G074840 PE=3 SV=1	[more]
A0A0A0L2I2	1.1e-151	77.86	PINc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G089000 PE=4 S...	[more]
A0A5A7SKL0	7.2e-151	74.31	Exosome complex exonuclease RRP44-like protein A OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BZS3	7.2e-151	74.31	exosome complex exonuclease RRP44 homolog A OS=Cucumis melo OX=3656 GN=LOC103495...	[more]
A0A6J1I5P7	1.9e-148	73.07	exosome complex exonuclease RRP44 homolog A-like OS=Cucurbita maxima OX=3661 GN=...	[more]

BLAST of CSPI03G05580 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8650154.1	1.7e-170	95.17	hypothetical protein Csa_010466 [Cucumis sativus]	[more]
XP_008454914.1	1.5e-150	74.31	PREDICTED: exosome complex exonuclease RRP44 homolog A [Cucumis melo] >KAA003128...	[more]
XP_011658916.2	2.5e-150	74.31	exosome complex exonuclease RRP44 homolog A [Cucumis sativus] >KAE8645869.1 hypo...	[more]
XP_038887941.1	1.8e-148	73.82	exosome complex exonuclease RRP44 homolog A [Benincasa hispida]	[more]
XP_022972336.1	4.0e-148	73.07	exosome complex exonuclease RRP44 homolog A-like [Cucurbita maxima]	[more]

BLAST of CSPI03G05580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G17510.1	9.0e-122	58.27	ribonuclease II family protein	[more]
AT2G17510.2	9.0e-122	58.27	ribonuclease II family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002716	PIN domain	SMART	SM00670	PIN_9	coord: 25..123 e-value: 3.4E-8 score: 43.2
None	No IPR available	GENE3D	2.40.50.700		coord: 323..387 e-value: 6.0E-19 score: 69.6
None	No IPR available	GENE3D	2.40.50.690		coord: 164..270 e-value: 2.7E-27 score: 96.8
None	No IPR available	GENE3D	3.40.50.1010		coord: 8..150 e-value: 2.8E-35 score: 123.6
None	No IPR available	PANTHER	PTHR23355:SF35	EXOSOME COMPLEX EXONUCLEASE RRP44	coord: 21..387
None	No IPR available	PANTHER	PTHR23355	RIBONUCLEASE	coord: 21..387
IPR041505	Dis3-like cold-shock domain 2	PFAM	PF17849	OB_Dis3	coord: 343..384 e-value: 5.3E-5 score: 23.1
IPR033771	Rrp44-like cold shock domain	PFAM	PF17216	Rrp44_CSD1	coord: 177..262 e-value: 1.9E-10 score: 40.7
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 173..385
IPR029060	PIN-like domain superfamily	SUPERFAMILY	88723	PIN domain-like	coord: 27..141

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G05580.1	CSPI03G05580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090501	RNA phosphodiester bond hydrolysis
biological_process	GO:0016075	rRNA catabolic process
cellular_component	GO:0000177	cytoplasmic exosome (RNase complex)
cellular_component	GO:0000176	nuclear exosome (RNase complex)
molecular_function	GO:0000175	3'-5'-exoribonuclease activity
molecular_function	GO:0004519	endonuclease activity