CSPI03G05560 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G05560
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Pectinesterase
Location	Chr3: 4705445 .. 4707404 (+)
RNA-Seq Expression	CSPI03G05560
Synteny	CSPI03G05560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATTATCTATTTTCTGTCACTATCATTCCCCATTCCATCTTATTCATCCCTCTTCTCATGGCTTCACATTACAAAATCCTCTTCATTTCCATAACTTTTTTCACACTTCTTTCAAATAATGTCTCTGCCAATCCCATCATCAAATGGTGCAAAACGACGCCCCATCCAAGAGTTTGCGTTTCCCTCATGTCCCCAATCAAACCTCCACCACAAAACAGACGGGAGTTTCGACAAATGGCCATTCAAACCACTTTGGAAAAAGCCGCCGAAGCCAGAGCTTACACTGCTCGCTTCGGTCCGACATGTAAAACCTCGCGCCAGAGAACCGCGTGGACTGACTGTTTCAAGCTCTACAACGACGTCGTTCTCCAATTAAACCGCACACTCCATTGCGTCGTTACGGATGAAGCAATCCACCGCCGCAGTTGTACGGATTTCGATGCGCAAACCTGGCTTAGCTCTGCTCTTACTGACATCGATTTATGCAACTCCGGCGCCGCGGATCTCAATGTGACGGATTTTATAACGCCGATCAAATGCTTGAATGTTTCGAAGATGATTAGTAATTGCTTGGCTATTAATGGTGGATTTTTGGAGGAAGAAGGGGTTAAATATGATGATGGTAGAAATGGGTCGTTTCCGATGTGGGTTTCGGAAGGTGATCGCAAGTTGTTGGAGTCGAGGCCGGGGAGGGTGAGGGCTAATTTGGTGGTGGCGAAAGATGGGTCGGGGACATTTCGGAGGGTTCAGGCGGCGATTGATGCGGCAGCGAGGAGGCGTGGGAGGGGGAGATTTATAATTTATGTAAAAAGAGGGGTATATAGAGAAAATATTGAAGTTGGAAATGATAATGGGAATATAATGTTGGTGGGAGATGGGATGAGATTTACGGTGATCACAAGTGGAAGAAGTGTCGCCGCCGGTTTCACCACCTTCAGTTCGGCAACCGCTGGTGGATTTCTCTCCTGGCCTTCTATATTTTGCACTTTATACCGTTTTAAAATAGCTATTTATTATTACACTCATATAGTTAAATTATTTCGGTAATTGTTTGGTAACCATTCTATTTCTATTACAAGTTCATCAATTTGTTAAAAACAAAATAAAAAACTTAAATAGATATCAAACACATAAGTTCAAGAAAATAGTAAATATTATCATAAAATCTAGGAACTAGTGTTTTCCTTTTTCTAAATGTGACTTAACTAATTTTACTGAGGTTTTAGGTATCCAAGGTCCAGGCTTCATAGCTCGAGACATAAGGTTCGTGAACACAGCCGGTCCACGAATGGGCCAAGCAGTGGCACTACGATCATCATCAGACCTATCAGTGTTTCATCGATGTAGCTTTGAAGGTTACCAAGACACACTAATGGTCCTTTCACAGAGGCAATTCTATAAACAATGTTACGTTTACGGTACAATCGACTTCATTTTCGGCAACGCGGCAGTAGTTTTACAAAATTGCATGATTTACGTTAGAAGGCCTTTAAAGGGGCAAGTCAATGTGATCACAGCCCAAGGTCGAGAGGACCCATTTCAAAACAGCGGAATATCAATCCACAACTCACAAATTCGAGCGGCGGCTGACTTGCGACCCATGGTTGGCTCGGTGAAGACGTACTTGGGTAGGCCATGGAAGAAGTATTCACGGACTGTGATAATGAGAAGCTACATTGATTGGTTGGTTAGCCCAGCCGGATGGTTGGCGTGGCAAAGTAGTAAATTTGCTCAAGCTACGTTGTATTATGGGGAGTACCGTAATATTGGGCCAAGAGCTTCAACGCGGTTTAGAGTTAAATGGCCTGGCTTTCATGTCATTAAAAGCCCAAATGTGGCATCTAAGTTTAGTGTTCAGAGACTTATTGCTGGCCAGACATGGCTTCCAGCCACCGGAGTTCCCTTCAAGCTTGGAGTTTGAGATTCAAACTTTCAAGGTTTGGAATTTGCTTCTCTCTC

mRNA sequence

CCATTATCTATTTTCTGTCACTATCATTCCCCATTCCATCTTATTCATCCCTCTTCTCATGGCTTCACATTACAAAATCCTCTTCATTTCCATAACTTTTTTCACACTTCTTTCAAATAATGTCTCTGCCAATCCCATCATCAAATGGTGCAAAACGACGCCCCATCCAAGAGTTTGCGTTTCCCTCATGTCCCCAATCAAACCTCCACCACAAAACAGACGGGAGTTTCGACAAATGGCCATTCAAACCACTTTGGAAAAAGCCGCCGAAGCCAGAGCTTACACTGCTCGCTTCGGTCCGACATGTAAAACCTCGCGCCAGAGAACCGCGTGGACTGACTGTTTCAAGCTCTACAACGACGTCGTTCTCCAATTAAACCGCACACTCCATTGCGTCGTTACGGATGAAGCAATCCACCGCCGCAGTTGTACGGATTTCGATGCGCAAACCTGGCTTAGCTCTGCTCTTACTGACATCGATTTATGCAACTCCGGCGCCGCGGATCTCAATGTGACGGATTTTATAACGCCGATCAAATGCTTGAATGTTTCGAAGATGATTAGTAATTGCTTGGCTATTAATGGTGGATTTTTGGAGGAAGAAGGGGTTAAATATGATGATGGTAGAAATGGGTCGTTTCCGATGTGGGTTTCGGAAGGTGATCGCAAGTTGTTGGAGTCGAGGCCGGGGAGGGTGAGGGCTAATTTGGTGGTGGCGAAAGATGGGTCGGGGACATTTCGGAGGGTTCAGGCGGCGATTGATGCGGCAGCGAGGAGGCGTGGGAGGGGGAGATTTATAATTTATGTAAAAAGAGGGGTATATAGAGAAAATATTGAAGTTGGAAATGATAATGGGAATATAATGTTGGTGGGAGATGGGATGAGATTTACGGTGATCACAAGTGGAAGAAGTGTCGCCGCCGGTTTCACCACCTTCAGTTCGGCAACCGCTGGTATCCAAGGTCCAGGCTTCATAGCTCGAGACATAAGGTTCGTGAACACAGCCGGTCCACGAATGGGCCAAGCAGTGGCACTACGATCATCATCAGACCTATCAGTGTTTCATCGATGTAGCTTTGAAGGTTACCAAGACACACTAATGGTCCTTTCACAGAGGCAATTCTATAAACAATGTTACGTTTACGGTACAATCGACTTCATTTTCGGCAACGCGGCAGTAGTTTTACAAAATTGCATGATTTACGTTAGAAGGCCTTTAAAGGGGCAAGTCAATGTGATCACAGCCCAAGGTCGAGAGGACCCATTTCAAAACAGCGGAATATCAATCCACAACTCACAAATTCGAGCGGCGGCTGACTTGCGACCCATGGTTGGCTCGGTGAAGACGTACTTGGGTAGGCCATGGAAGAAGTATTCACGGACTGTGATAATGAGAAGCTACATTGATTGGTTGGTTAGCCCAGCCGGATGGTTGGCGTGGCAAAGTAGTAAATTTGCTCAAGCTACGTTGTATTATGGGGAGTACCGTAATATTGGGCCAAGAGCTTCAACGCGGTTTAGAGTTAAATGGCCTGGCTTTCATGTCATTAAAAGCCCAAATGTGGCATCTAAGTTTAGTGTTCAGAGACTTATTGCTGGCCAGACATGGCTTCCAGCCACCGGAGTTCCCTTCAAGCTTGGAGTTTGAGATTCAAACTTTCAAGGTTTGGAATTTGCTTCTCTCTC

Coding sequence (CDS)

ATGGCTTCACATTACAAAATCCTCTTCATTTCCATAACTTTTTTCACACTTCTTTCAAATAATGTCTCTGCCAATCCCATCATCAAATGGTGCAAAACGACGCCCCATCCAAGAGTTTGCGTTTCCCTCATGTCCCCAATCAAACCTCCACCACAAAACAGACGGGAGTTTCGACAAATGGCCATTCAAACCACTTTGGAAAAAGCCGCCGAAGCCAGAGCTTACACTGCTCGCTTCGGTCCGACATGTAAAACCTCGCGCCAGAGAACCGCGTGGACTGACTGTTTCAAGCTCTACAACGACGTCGTTCTCCAATTAAACCGCACACTCCATTGCGTCGTTACGGATGAAGCAATCCACCGCCGCAGTTGTACGGATTTCGATGCGCAAACCTGGCTTAGCTCTGCTCTTACTGACATCGATTTATGCAACTCCGGCGCCGCGGATCTCAATGTGACGGATTTTATAACGCCGATCAAATGCTTGAATGTTTCGAAGATGATTAGTAATTGCTTGGCTATTAATGGTGGATTTTTGGAGGAAGAAGGGGTTAAATATGATGATGGTAGAAATGGGTCGTTTCCGATGTGGGTTTCGGAAGGTGATCGCAAGTTGTTGGAGTCGAGGCCGGGGAGGGTGAGGGCTAATTTGGTGGTGGCGAAAGATGGGTCGGGGACATTTCGGAGGGTTCAGGCGGCGATTGATGCGGCAGCGAGGAGGCGTGGGAGGGGGAGATTTATAATTTATGTAAAAAGAGGGGTATATAGAGAAAATATTGAAGTTGGAAATGATAATGGGAATATAATGTTGGTGGGAGATGGGATGAGATTTACGGTGATCACAAGTGGAAGAAGTGTCGCCGCCGGTTTCACCACCTTCAGTTCGGCAACCGCTGGTATCCAAGGTCCAGGCTTCATAGCTCGAGACATAAGGTTCGTGAACACAGCCGGTCCACGAATGGGCCAAGCAGTGGCACTACGATCATCATCAGACCTATCAGTGTTTCATCGATGTAGCTTTGAAGGTTACCAAGACACACTAATGGTCCTTTCACAGAGGCAATTCTATAAACAATGTTACGTTTACGGTACAATCGACTTCATTTTCGGCAACGCGGCAGTAGTTTTACAAAATTGCATGATTTACGTTAGAAGGCCTTTAAAGGGGCAAGTCAATGTGATCACAGCCCAAGGTCGAGAGGACCCATTTCAAAACAGCGGAATATCAATCCACAACTCACAAATTCGAGCGGCGGCTGACTTGCGACCCATGGTTGGCTCGGTGAAGACGTACTTGGGTAGGCCATGGAAGAAGTATTCACGGACTGTGATAATGAGAAGCTACATTGATTGGTTGGTTAGCCCAGCCGGATGGTTGGCGTGGCAAAGTAGTAAATTTGCTCAAGCTACGTTGTATTATGGGGAGTACCGTAATATTGGGCCAAGAGCTTCAACGCGGTTTAGAGTTAAATGGCCTGGCTTTCATGTCATTAAAAGCCCAAATGTGGCATCTAAGTTTAGTGTTCAGAGACTTATTGCTGGCCAGACATGGCTTCCAGCCACCGGAGTTCCCTTCAAGCTTGGAGTTTGA

Protein sequence

MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLEEEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV*

Homology

[Close]

BLAST of CSPI03G05560 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 8.2e-163
Identity = 297/538 (55.20%), Postives = 370/538 (68.77%), Query Frame = 0


Query: 1   MASHYKILFISITFFTLLS----NNVSANPIIKWCKTTPHPRVCVSLMSPIK--PPPQNR 60
           M     ILF+ +    LL       V+      WC  TP+P  C            P   
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQL 63

Query: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120
            EFR M ++  +++A  ARA     G  C  S+++    DC  LY D ++QLNRTLH  V
Sbjct: 64  SEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLH-GV 123

Query: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPI-KCLNVSKMISNCLA 180
           + +A   +SCTDFDAQTWLS+ALT+ + C  G++DLNVTDFITPI     +S +ISNCLA
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLA 183

Query: 181 INGGFLEEEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAA 240
           +NG  L              FP W+S  D++LL +    VRANLVVAKDGSG F  VQAA
Sbjct: 184 VNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA----VRANLVVAKDGSGHFNTVQAA 243

Query: 241 IDAAARRR-GRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTT 300
           ID A RR+   GRF+IYVKRG+Y+ENI V  +N +IMLVGDGMR T+IT GRSV  G+TT
Sbjct: 244 IDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTT 303

Query: 301 FSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQ 360
           ++SATAGI+G  FIA+ I F NTAGP  GQAVALRSSSDLS+F++CS EGYQDTLMV SQ
Sbjct: 304 YNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQ 363

Query: 361 RQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHN 420
           RQFY++CY+YGT+DFIFGNAA V QNC+I  RRPLKGQ NVITAQGR DPFQN+GISIHN
Sbjct: 364 RQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHN 423

Query: 421 SQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAW-QSSKFAQATL 480
           S+I  A DL+P+VG+VKTY+GRPW K+SRTV++++Y+D +VSP GW  W + S F   TL
Sbjct: 424 SRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTL 483

Query: 481 YYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
           +Y EY+N GP +STR+RV W GFHV+   + AS F+V + IAG  WLP TG+PF  G+
Sbjct: 484 FYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of CSPI03G05560 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 8.5e-160
Identity = 286/521 (54.89%), Postives = 372/521 (71.40%), Query Frame = 0


Query: 18  LSNNVSANPIIKWCKTTPHPRVC-----VSLMSPIK-PPPQNRREFRQMAIQTTLEKAAE 77
           ++N   +  +  WC  TPH   C      S  + I   PP+ R EF +M ++  L++A  
Sbjct: 75  INNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVI 134

Query: 78  ARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIHRRSCTDFDAQT 137
             + T +FGP+C  ++++ AW+DC  L+ + V QLNRTL   +   A     CTDFDAQT
Sbjct: 135 THSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLK-GLNPAASSDVKCTDFDAQT 194

Query: 138 WLSSALTDIDLCNSGAADLNVTDFITP-IKCLNVSKMISNCLAINGGFLEEEGVKYDDGR 197
           WLS+A T+I+ C SG+ DLNV+DF+ P I   N+S +I NCLA+NG  +++         
Sbjct: 195 WLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANH 254

Query: 198 NGSFPMWVSEGDRKLLESRP-GRVRANLVVAKDGSGTFRRVQAAIDAAARRRGRGRFIIY 257
              FP WVS  +R+LL S    +   +LVVA+D SG FR +QAAI+ AARRR + RF+IY
Sbjct: 255 KEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIY 314

Query: 258 VKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPGFIARD 317
           VK+GVYRENI+VGNDN NIMLVGDG R T+ITSGRSV  G+TT++SAT G  G  F+A+D
Sbjct: 315 VKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKD 374

Query: 318 IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIF 377
           + F+NTAGP  GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CY+ GTIDFIF
Sbjct: 375 MTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIF 434

Query: 378 GNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLRPMVGSVK 437
           GNAAVV QNCMI VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA+DL+P++ + K
Sbjct: 435 GNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYK 494

Query: 438 TYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAW-QSSKFAQATLYYGEYRNIGPRASTRFR 497
           TYLGRPW+ YSR  IM++YID  +SP GW  W + S FA  T++YGEY+N GP +STR+R
Sbjct: 495 TYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWR 554

Query: 498 VKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
           V+W GFH I S  VAS+F+V  LIAG +WLPATGVPFK G+
Sbjct: 555 VRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of CSPI03G05560 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 3.0e-157
Identity = 293/525 (55.81%), Postives = 366/525 (69.71%), Query Frame = 0


Query: 7   ILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLM---SPIKPPPQNRREFRQMAIQ 66
           IL + +   T +++  S N I  WC  TP+P  C       S  + P Q   EFR + ++
Sbjct: 19  ILLLCLRPLTTVADGNSTN-IDGWCDKTPYPYPCKRYFIKHSGFRLPTQ-ISEFRVLLVE 78

Query: 67  TTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIHRRS 126
             +++A  A          C   +++    DC  LY D V+QLNRTL  V +      R 
Sbjct: 79  AAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTG---RR 138

Query: 127 CTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPI-KCLNVSKMISNCLAINGGFLEEE 186
           CTDFDAQTWLS+ALT+ + C  G++DLNV+DF TPI     +S +ISNCLA+NG  L   
Sbjct: 139 CTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAG 198

Query: 187 GVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARRR- 246
                 G +  FP WVS  +R+LL+ +   VRANLVVAKDGSG F+ VQAAID A RR+ 
Sbjct: 199 KNDSTTGDSKGFPTWVSRKERRLLQLQ--SVRANLVVAKDGSGHFKTVQAAIDVAGRRKV 258

Query: 247 GRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQ 306
             GRF+IYVKRG+Y+EN+ V  +N NIMLVGDGMR+T+IT GRSV  G+TT+SSATAGI+
Sbjct: 259 TSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIE 318

Query: 307 GPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYV 366
           G  FIA+ I F NTAGP  GQAVALRSSSDLS+F+RCS EGYQDTLMV SQRQFY++CY+
Sbjct: 319 GLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYI 378

Query: 367 YGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADL 426
           YGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIHNS I  A DL
Sbjct: 379 YGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDL 438

Query: 427 RPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAW-QSSKFAQATLYYGEYRNIG 486
           +P+V SVKTY+GRPW  YSRTV++++YID +VSP GW  W + S +   TL+Y EY+NIG
Sbjct: 439 KPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIG 498

Query: 487 PRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPF 526
           P +STR+RV+W GFHV+   + AS FSV + IAG  WLP +G+PF
Sbjct: 499 PASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

[Close]

BLAST of CSPI03G05560 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 4.1e-138
Identity = 260/527 (49.34%), Postives = 350/527 (66.41%), Query Frame = 0


Query: 7   ILFISITFFTLLSNNVSANP--IIKWCKTTPHPRVCVSLMSPIKPPPQNRR--EFRQMAI 66
           ++ +S+  F+L   +   +P  +  WC  TP+P+ C   ++        ++  +F ++++
Sbjct: 6   LITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISL 65

Query: 67  QTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIHRR 126
           Q  LE+A  A++ T   G  C+  R++ AW DC +LY   VL+LN+T +           
Sbjct: 66  QLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSN--------SSP 125

Query: 127 SCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLEEE 186
            CT  D QTWLS+ALT+++ C +   DL V +++ P+   NV+K+ISN L++N     E 
Sbjct: 126 GCTKVDKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVPYNEP 185

Query: 187 GVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARRRG 246
              Y DG    FP WV  GDRKLL++ P   RAN+VVA+DGSG  + +Q A+ AAA R G
Sbjct: 186 --SYKDG----FPTWVKPGDRKLLQTTP---RANIVVAQDGSGNVKTIQEAV-AAASRAG 245

Query: 247 RGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQG 306
             R++IY+K G Y ENIEV     NIM VGDG+  T+IT  +SV  G TTF SAT  + G
Sbjct: 246 GSRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVG 305

Query: 307 PGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVY 366
             FIARDI   NTAGP   QAVALRS SDLSVF+RCSFEGYQDTL V SQRQFY++C +Y
Sbjct: 306 DNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIY 365

Query: 367 GTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLR 426
           GT+DFIFGNAAVVLQNC I+ R+P   + N +TAQGR DP Q++GI IHN ++ AA+DL+
Sbjct: 366 GTVDFIFGNAAVVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLK 425

Query: 427 PMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIGPR 486
           P+  SVKT+LGRPWK+YSRTV +++++D L++PAGW+ W S  FA  TLYY EY N GP 
Sbjct: 426 PVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEW-SGDFALNTLYYAEYMNTGPG 485

Query: 487 ASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
           +ST  RVKW G+HV+ SP+  S+F+V   IAG +WLPAT VPF  G+
Sbjct: 486 SSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of CSPI03G05560 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 485.7 bits (1249), Expect = 6.6e-136
Identity = 254/502 (50.60%), Postives = 335/502 (66.73%), Query Frame = 0


Query: 30  WCKTTPHPRVCVSLM--SPIKPPPQNRREFRQMAIQTTLEKAAEARAYTARFGPTCKTSR 89
           WC  TP+P+ C   +  +    P ++  EF +++++  L++A  A+ +    GP C+ +R
Sbjct: 33  WCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCRDTR 92

Query: 90  QRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGA 149
           ++ AW DC KLY+  V ++N T+   V         C+  DAQTWLS+ALT++D C +G 
Sbjct: 93  EKAAWEDCIKLYDLTVSKINETMDPNV--------KCSKLDAQTWLSTALTNLDTCRAGF 152

Query: 150 ADLNVTDFITPIKCLNVSKMISNCLAINGGFLEEEGVKYDDGRNGSFPMWVSEGDRKLLE 209
            +L VTD + P+   NVS ++ N LAIN     +    Y       FP WV  GDRKLL+
Sbjct: 153 LELGVTDIVLPLMSNNVSNLLCNTLAIN-----KVPFNYTPPEKDGFPSWVKPGDRKLLQ 212

Query: 210 SRPGRVRANLVVAKDGSGTFRRVQAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGN 269
           S     + N VVAKDGSG F+ ++ AIDAA+   G GRF+IYVK+GVY EN+E+     N
Sbjct: 213 S--STPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENLEIRKK--N 272

Query: 270 IMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALR 329
           +ML GDG+  T+IT  +SV  G TTF+SAT    G GFIAR I F NTAG    QAVALR
Sbjct: 273 VMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALR 332

Query: 330 SSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPL 389
           S SDLSVF++CSFE YQDTL V S RQFY+ C VYGT+DFIFGNAA VLQNC I+ RRP 
Sbjct: 333 SGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP- 392

Query: 390 KGQVNVITAQGREDPFQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRS 449
           + + N ITAQGR DP QN+GI IHNS++ AA+DLRP++GS KTYLGRPW++YSRTV M++
Sbjct: 393 RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKT 452

Query: 450 YIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFS 509
            +D L+ P GWL W  + FA  TL+Y E++N GP AST  RV WPGF V+ S + ASKF+
Sbjct: 453 SLDSLIDPRGWLEWDGN-FALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFT 511

Query: 510 VQRLIAGQTWLPATGVPFKLGV 530
           V   +AG +W+P++ VPF  G+
Sbjct: 513 VGTFLAGGSWIPSS-VPFTSGL 511

[Close]

BLAST of CSPI03G05560 vs. ExPASy TrEMBL
Match: A0A0A0L330 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G088980 PE=3 SV=1)

HSP 1 Score: 1071.2 bits (2769), Expect = 1.4e-309
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0


Query: 1   MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQM 60
           MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQM
Sbjct: 1   MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQM 60

Query: 61  AIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIH 120
           AIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIH
Sbjct: 61  AIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIH 120

Query: 121 RRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLE 180
           RRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLE
Sbjct: 121 RRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLE 180

Query: 181 EEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARR 240
           EEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARR
Sbjct: 181 EEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARR 240

Query: 241 RGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI 300
           RGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI
Sbjct: 241 RGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI 300

Query: 301 QGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCY 360
           QGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCY
Sbjct: 301 QGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCY 360

Query: 361 VYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAAD 420
           VYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAAD
Sbjct: 361 VYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAAD 420

Query: 421 LRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIG 480
           LRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIG
Sbjct: 421 LRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIG 480

Query: 481 PRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
           PRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV
Sbjct: 481 PRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 529

[Close]

BLAST of CSPI03G05560 vs. ExPASy TrEMBL
Match: A0A1S3BUR4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493398 PE=3 SV=1)

HSP 1 Score: 1009.2 bits (2608), Expect = 6.3e-291
Identity = 504/533 (94.56%), Postives = 516/533 (96.81%), Query Frame = 0


Query: 1   MASHYK-ILFISIT-FFTLLSNNVSA-NPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREF 60
           MASHYK ILFISIT  FT+LSNNVSA NPIIKWCKTTPHP+VC+S MSPIKPPPQNR EF
Sbjct: 1   MASHYKIILFISITLLFTILSNNVSANNPIIKWCKTTPHPKVCISFMSPIKPPPQNREEF 60

Query: 61  RQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDE 120
           RQ+AIQTTLEKA EA+AYTARFGPTCKT+RQRTAWTDCFKLYNDVV QLNRTL C+VTDE
Sbjct: 61  RQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQCLVTDE 120

Query: 121 AIHRR-SCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAING 180
            IHRR +CTDFDAQTWLSSALTDIDLCNSGAADLNVTDFI PIKCLNVSKMISNCLAING
Sbjct: 121 TIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNCLAING 180

Query: 181 GFLEEEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDA 240
           GFLEEEGVKYDDG NGSFPMWVSEGDRKLLESRP RVRANLVVAKDGSG FRRVQAAIDA
Sbjct: 181 GFLEEEGVKYDDGGNGSFPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFRRVQAAIDA 240

Query: 241 AARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSA 300
           AARRRGRGRF+IYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSA
Sbjct: 241 AARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSA 300

Query: 301 TAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFY 360
           TAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFY
Sbjct: 301 TAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFY 360

Query: 361 KQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIR 420
           KQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIR
Sbjct: 361 KQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIR 420

Query: 421 AAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEY 480
           AAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYID LVSPAGWLAWQSSKFAQATLYYGEY
Sbjct: 421 AAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQATLYYGEY 480

Query: 481 RNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
            NIGPRASTRFRVKWPGFHVIKSPNVASKFSV+RLIAGQTWLPATGVPFKLGV
Sbjct: 481 HNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFKLGV 533

[Close]

BLAST of CSPI03G05560 vs. ExPASy TrEMBL
Match: A0A5A7TVK4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G001080 PE=3 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 6.3e-267
Identity = 461/487 (94.66%), Postives = 471/487 (96.71%), Query Frame = 0


Query: 44  MSPIKPPPQNRREFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVV 103
           MSPIKPPPQNR EFRQ+AIQTTLEKA EA+AYTARFGPTCKT+RQRTAWTDCFKLYNDVV
Sbjct: 1   MSPIKPPPQNREEFRQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVV 60

Query: 104 LQLNRTLHCVVTDEAIHRR-SCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCL 163
            QLNRTL C+VTDE IHRR +CTDFDAQTWLSSALTDIDLCNSGAADLNVTDFI PIKCL
Sbjct: 61  FQLNRTLQCLVTDETIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCL 120

Query: 164 NVSKMISNCLAINGGFLEEEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKD 223
           NVSKMISNCLAINGGF+EEEGVKYDDG NGSFPMWVSEGDRKLLESRP RVRANLVVAKD
Sbjct: 121 NVSKMISNCLAINGGFIEEEGVKYDDGGNGSFPMWVSEGDRKLLESRPRRVRANLVVAKD 180

Query: 224 GSGTFRRVQAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITS 283
           GSG FRRVQAAIDAAARRRGRGRF+IYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITS
Sbjct: 181 GSGKFRRVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITS 240

Query: 284 GRSVAAGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEG 343
           GRSVAAGFTTFS     IQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEG
Sbjct: 241 GRSVAAGFTTFS-----IQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEG 300

Query: 344 YQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDP 403
           YQDTLMVLSQRQFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP
Sbjct: 301 YQDTLMVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDP 360

Query: 404 FQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQ 463
           FQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYID LVSPAGWLAWQ
Sbjct: 361 FQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQ 420

Query: 464 SSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATG 523
           SSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSV+RLIAGQTWLPATG
Sbjct: 421 SSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATG 480

Query: 524 VPFKLGV 530
           VPFKLGV
Sbjct: 481 VPFKLGV 482

[Close]

BLAST of CSPI03G05560 vs. ExPASy TrEMBL
Match: A0A6J1EW59 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438452 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.8e-244
Identity = 429/537 (79.89%), Postives = 465/537 (86.59%), Query Frame = 0


Query: 1   MASHYKILFISITFFTLLSNN------VSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNR 60
           MA H +I F SI  F LLSN+       S N I KWC TTPHP+VC+  MSP+K PPQNR
Sbjct: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSGNTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60

Query: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120
            EFR+MAI+TTLE AA+A+ Y + FGPTCKT+RQR AWTDCF LYND V QLNRT+ C+ 
Sbjct: 61  LEFRKMAIKTTLEGAAKAQNYVSLFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCL- 120

Query: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAI 180
            D     R CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFITPIKC NVSKMISNCLAI
Sbjct: 121 -DGGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAI 180

Query: 181 NGGFLEEEGVKYDDG----RNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRV 240
           NG FL+EE      G    +NG FP WVS GDRKLL+S   RVRA+LVVAKDGSG FR V
Sbjct: 181 NGRFLQEETKSRTTGTEEYQNG-FPTWVSSGDRKLLQS--ARVRAHLVVAKDGSGKFRTV 240

Query: 241 QAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RF+IY+KRGVY+ENIEVGNDN NIMLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGF 300

Query: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQAT 480
           HNSQIRAAADL+PM+GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480

Query: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKL 528
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKF+V+RLIAG TWLPATGVPFKL
Sbjct: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKL 532

[Close]

BLAST of CSPI03G05560 vs. ExPASy TrEMBL
Match: A0A6J1JCM7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483275 PE=3 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 7.5e-244
Identity = 429/537 (79.89%), Postives = 466/537 (86.78%), Query Frame = 0


Query: 1   MASHYKILFISITFFTLLSNN------VSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNR 60
           MA H +I F SI  F LLSN+       SAN I KWC TTPHP+VC+  MSP+K PPQNR
Sbjct: 1   MAPHIQIAFFSIALFALLSNSSSSPTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 60

Query: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120
            EFR+MAI+TTLE AA+A+ Y +RFGPTCKT+RQR AWTDCF LYND V QLNRT+ C+ 
Sbjct: 61  IEFRKMAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIRCLH 120

Query: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAI 180
               +  R CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFITPIKC NVS+MISNCLAI
Sbjct: 121 AGTRL--RHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSRMISNCLAI 180

Query: 181 NGGFLEEEGVKYDDG----RNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRV 240
           NG FLE E      G    +NG FP WVS GDRKLL+S   RVRA+LVVAKDGSG FR V
Sbjct: 181 NGRFLEGETKSRTTGAEEYQNG-FPRWVSSGDRKLLQS--ARVRAHLVVAKDGSGKFRTV 240

Query: 241 QAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RF+IY+KRGVYRENIE+GNDN NIMLVGDG+RFTVITSGRSVAAGF
Sbjct: 241 QAAINAAARRRGRSRFVIYIKRGVYRENIEIGNDNANIMLVGDGIRFTVITSGRSVAAGF 300

Query: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 301 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360

Query: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 420

Query: 421 HNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQAT 480
           HNSQIRAAADL+PM+GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ ++FAQ T
Sbjct: 421 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 480

Query: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKL 528
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKF+V+RLIAG TWLPATGVPFKL
Sbjct: 481 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKL 532

[Close]

BLAST of CSPI03G05560 vs. NCBI nr
Match: XP_011650542.1 (probable pectinesterase/pectinesterase inhibitor 59 [Cucumis sativus] >KGN56178.1 hypothetical protein Csa_011446 [Cucumis sativus])

HSP 1 Score: 1071.2 bits (2769), Expect = 2.8e-309
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0


Query: 1   MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQM 60
           MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQM
Sbjct: 1   MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQM 60

Query: 61  AIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIH 120
           AIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIH
Sbjct: 61  AIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIH 120

Query: 121 RRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLE 180
           RRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLE
Sbjct: 121 RRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLE 180

Query: 181 EEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARR 240
           EEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARR
Sbjct: 181 EEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARR 240

Query: 241 RGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI 300
           RGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI
Sbjct: 241 RGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGI 300

Query: 301 QGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCY 360
           QGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCY
Sbjct: 301 QGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCY 360

Query: 361 VYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAAD 420
           VYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAAD
Sbjct: 361 VYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAAD 420

Query: 421 LRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIG 480
           LRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIG
Sbjct: 421 LRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIG 480

Query: 481 PRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
           PRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV
Sbjct: 481 PRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 529

[Close]

BLAST of CSPI03G05560 vs. NCBI nr
Match: XP_008452338.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo] >XP_016901218.1 PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo])

HSP 1 Score: 1009.2 bits (2608), Expect = 1.3e-290
Identity = 504/533 (94.56%), Postives = 516/533 (96.81%), Query Frame = 0


Query: 1   MASHYK-ILFISIT-FFTLLSNNVSA-NPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREF 60
           MASHYK ILFISIT  FT+LSNNVSA NPIIKWCKTTPHP+VC+S MSPIKPPPQNR EF
Sbjct: 1   MASHYKIILFISITLLFTILSNNVSANNPIIKWCKTTPHPKVCISFMSPIKPPPQNREEF 60

Query: 61  RQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDE 120
           RQ+AIQTTLEKA EA+AYTARFGPTCKT+RQRTAWTDCFKLYNDVV QLNRTL C+VTDE
Sbjct: 61  RQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVVFQLNRTLQCLVTDE 120

Query: 121 AIHRR-SCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAING 180
            IHRR +CTDFDAQTWLSSALTDIDLCNSGAADLNVTDFI PIKCLNVSKMISNCLAING
Sbjct: 121 TIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCLNVSKMISNCLAING 180

Query: 181 GFLEEEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDA 240
           GFLEEEGVKYDDG NGSFPMWVSEGDRKLLESRP RVRANLVVAKDGSG FRRVQAAIDA
Sbjct: 181 GFLEEEGVKYDDGGNGSFPMWVSEGDRKLLESRPRRVRANLVVAKDGSGKFRRVQAAIDA 240

Query: 241 AARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSA 300
           AARRRGRGRF+IYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSA
Sbjct: 241 AARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSA 300

Query: 301 TAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFY 360
           TAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFY
Sbjct: 301 TAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFY 360

Query: 361 KQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIR 420
           KQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIR
Sbjct: 361 KQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIR 420

Query: 421 AAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYGEY 480
           AAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYID LVSPAGWLAWQSSKFAQATLYYGEY
Sbjct: 421 AAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQATLYYGEY 480

Query: 481 RNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
            NIGPRASTRFRVKWPGFHVIKSPNVASKFSV+RLIAGQTWLPATGVPFKLGV
Sbjct: 481 HNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATGVPFKLGV 533

[Close]

BLAST of CSPI03G05560 vs. NCBI nr
Match: XP_038905752.1 (probable pectinesterase/pectinesterase inhibitor 60 [Benincasa hispida])

HSP 1 Score: 930.6 bits (2404), Expect = 5.9e-267
Identity = 463/535 (86.54%), Postives = 489/535 (91.40%), Query Frame = 0


Query: 1   MASHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNRREFRQM 60
           MASH KILF SIT F++LS NVS+N I KWCKTTPHP++C+  MSPIKPPPQNR EFR+M
Sbjct: 1   MASHNKILFFSITLFSILS-NVSSNSINKWCKTTPHPKICIYFMSPIKPPPQNRPEFRKM 60

Query: 61  AIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIH 120
           AI+T L+KA EA+ Y  RFGP+CKT+RQR AWTDC KLYNDVV QLNRTL CV++D  I 
Sbjct: 61  AIRTALQKATEAQTYATRFGPSCKTTRQRAAWTDCLKLYNDVVFQLNRTLQCVISDGTIR 120

Query: 121 RRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAINGGFLE 180
            R CTDFDAQTWLSSALTDIDLC SGAADLNVTDFI PIKC NVSKMISNCLA+NG FLE
Sbjct: 121 HRRCTDFDAQTWLSSALTDIDLCVSGAADLNVTDFIAPIKCQNVSKMISNCLAVNGRFLE 180

Query: 181 EEGVK------YDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAI 240
           EE  K       +D R+G FPMWVS+GDRKLL+S   RVRANLVVAKDGSG FR VQAAI
Sbjct: 181 EEAKKSETKIGVEDDRDGPFPMWVSDGDRKLLQS-SARVRANLVVAKDGSGKFRTVQAAI 240

Query: 241 DAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFS 300
           DAAARRRGRGRF+IYVKRGVYRENIEVGNDNGN+MLVGDGMRFTVITSGRSVAAGFTTFS
Sbjct: 241 DAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNLMLVGDGMRFTVITSGRSVAAGFTTFS 300

Query: 301 SATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQ 360
           SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQ
Sbjct: 301 SATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQ 360

Query: 361 FYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQ 420
           FYKQCYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQ
Sbjct: 361 FYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQ 420

Query: 421 IRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQATLYYG 480
           IRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYID LVSPAGWLAWQSSKFAQATLYYG
Sbjct: 421 IRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQSSKFAQATLYYG 480

Query: 481 EYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
           EYRNIGPRA TR+RV+WPGFHVIKSPNVASKF+VQRLIAGQTWLPATGVPFKLGV
Sbjct: 481 EYRNIGPRAFTRYRVRWPGFHVIKSPNVASKFTVQRLIAGQTWLPATGVPFKLGV 533

[Close]

BLAST of CSPI03G05560 vs. NCBI nr
Match: KAA0046994.1 (putative pectinesterase/pectinesterase inhibitor 59 [Cucumis melo var. makuwa] >TYK04788.1 putative pectinesterase/pectinesterase inhibitor 59 [Cucumis melo var. makuwa])

HSP 1 Score: 929.5 bits (2401), Expect = 1.3e-266
Identity = 461/487 (94.66%), Postives = 471/487 (96.71%), Query Frame = 0


Query: 44  MSPIKPPPQNRREFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVV 103
           MSPIKPPPQNR EFRQ+AIQTTLEKA EA+AYTARFGPTCKT+RQRTAWTDCFKLYNDVV
Sbjct: 1   MSPIKPPPQNREEFRQVAIQTTLEKADEAKAYTARFGPTCKTTRQRTAWTDCFKLYNDVV 60

Query: 104 LQLNRTLHCVVTDEAIHRR-SCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCL 163
            QLNRTL C+VTDE IHRR +CTDFDAQTWLSSALTDIDLCNSGAADLNVTDFI PIKCL
Sbjct: 61  FQLNRTLQCLVTDETIHRRPTCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIAPIKCL 120

Query: 164 NVSKMISNCLAINGGFLEEEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKD 223
           NVSKMISNCLAINGGF+EEEGVKYDDG NGSFPMWVSEGDRKLLESRP RVRANLVVAKD
Sbjct: 121 NVSKMISNCLAINGGFIEEEGVKYDDGGNGSFPMWVSEGDRKLLESRPRRVRANLVVAKD 180

Query: 224 GSGTFRRVQAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITS 283
           GSG FRRVQAAIDAAARRRGRGRF+IYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITS
Sbjct: 181 GSGKFRRVQAAIDAAARRRGRGRFVIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITS 240

Query: 284 GRSVAAGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEG 343
           GRSVAAGFTTFS     IQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEG
Sbjct: 241 GRSVAAGFTTFS-----IQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEG 300

Query: 344 YQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDP 403
           YQDTLMVLSQRQFYKQCY+YGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDP
Sbjct: 301 YQDTLMVLSQRQFYKQCYIYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDP 360

Query: 404 FQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQ 463
           FQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYID LVSPAGWLAWQ
Sbjct: 361 FQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPAGWLAWQ 420

Query: 464 SSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATG 523
           SSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSV+RLIAGQTWLPATG
Sbjct: 421 SSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVRRLIAGQTWLPATG 480

Query: 524 VPFKLGV 530
           VPFKLGV
Sbjct: 481 VPFKLGV 482

[Close]

BLAST of CSPI03G05560 vs. NCBI nr
Match: XP_023520442.1 (probable pectinesterase/pectinesterase inhibitor 59 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 855.1 bits (2208), Expect = 3.1e-244
Identity = 429/537 (79.89%), Postives = 467/537 (86.96%), Query Frame = 0


Query: 1   MASHYKILFISITFFTLLSNN------VSANPIIKWCKTTPHPRVCVSLMSPIKPPPQNR 60
           MA H +I F SI  F LLSN+       SAN I KWC TTPHP+VC+  MSP+K PPQNR
Sbjct: 51  MAPHIQIAFFSIALFALLSNSSSSLTKTSANTINKWCNTTPHPKVCIFFMSPLKSPPQNR 110

Query: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120
            +FR++AI+TTLE AA+A+ Y +RFGPTCKT+RQR AWTDCF LYND V QLNRT+ C+ 
Sbjct: 111 LQFRKLAIKTTLEGAAKAQNYVSRFGPTCKTTRQRAAWTDCFNLYNDTVFQLNRTIQCL- 170

Query: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPIKCLNVSKMISNCLAI 180
            D     R CTDFDAQTWLSSALT+ID+C+SGAADLNVTDFITPIKC NVSKMISNCLAI
Sbjct: 171 -DAGTRLRHCTDFDAQTWLSSALTNIDMCSSGAADLNVTDFITPIKCQNVSKMISNCLAI 230

Query: 181 NGGFLEEEGVKYDDG----RNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRV 240
           NG FLEEE      G    +NG FP W+S GDRKLL+S   RVRA+LVVAKDGSG FR V
Sbjct: 231 NGRFLEEETKSRTTGTEEYQNG-FPTWLSTGDRKLLQS--ARVRAHLVVAKDGSGKFRTV 290

Query: 241 QAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGF 300
           QAAI+AAARRRGR RF+IY+KRGVY+ENIEVGNDN NIMLVGDG+RFTVITSGRSVAAGF
Sbjct: 291 QAAINAAARRRGRSRFVIYIKRGVYKENIEVGNDNANIMLVGDGIRFTVITSGRSVAAGF 350

Query: 301 TTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 360
           TTF+SATAGIQGPGF+AR IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL
Sbjct: 351 TTFNSATAGIQGPGFMARGIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVL 410

Query: 361 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISI 420
           SQRQFYKQCYVYGTIDFIFGNAAVVLQNC+IYVRRPLKGQVNVITAQGREDPFQNSGISI
Sbjct: 411 SQRQFYKQCYVYGTIDFIFGNAAVVLQNCLIYVRRPLKGQVNVITAQGREDPFQNSGISI 470

Query: 421 HNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAWQSSKFAQAT 480
           HNSQIRAAADL+PM+GSVKTYLGRPWKKYSRTVIMRSYID LVSP GWLAWQ ++FAQ T
Sbjct: 471 HNSQIRAAADLQPMIGSVKTYLGRPWKKYSRTVIMRSYIDSLVSPMGWLAWQGTRFAQDT 530

Query: 481 LYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKL 528
           LYYGEYRN GPRASTR RV+WPGFH I + NVASKF+V+RLIAG TWLPATGVPFKL
Sbjct: 531 LYYGEYRNFGPRASTRLRVRWPGFHAITTRNVASKFTVRRLIAGHTWLPATGVPFKL 582

[Close]

BLAST of CSPI03G05560 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 575.1 bits (1481), Expect = 5.8e-164
Identity = 297/538 (55.20%), Postives = 370/538 (68.77%), Query Frame = 0


Query: 1   MASHYKILFISITFFTLLS----NNVSANPIIKWCKTTPHPRVCVSLMSPIK--PPPQNR 60
           M     ILF+ +    LL       V+      WC  TP+P  C            P   
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQL 63

Query: 61  REFRQMAIQTTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVV 120
            EFR M ++  +++A  ARA     G  C  S+++    DC  LY D ++QLNRTLH  V
Sbjct: 64  SEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLH-GV 123

Query: 121 TDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPI-KCLNVSKMISNCLA 180
           + +A   +SCTDFDAQTWLS+ALT+ + C  G++DLNVTDFITPI     +S +ISNCLA
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLA 183

Query: 181 INGGFLEEEGVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAA 240
           +NG  L              FP W+S  D++LL +    VRANLVVAKDGSG F  VQAA
Sbjct: 184 VNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA----VRANLVVAKDGSGHFNTVQAA 243

Query: 241 IDAAARRR-GRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTT 300
           ID A RR+   GRF+IYVKRG+Y+ENI V  +N +IMLVGDGMR T+IT GRSV  G+TT
Sbjct: 244 IDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTT 303

Query: 301 FSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQ 360
           ++SATAGI+G  FIA+ I F NTAGP  GQAVALRSSSDLS+F++CS EGYQDTLMV SQ
Sbjct: 304 YNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQ 363

Query: 361 RQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHN 420
           RQFY++CY+YGT+DFIFGNAA V QNC+I  RRPLKGQ NVITAQGR DPFQN+GISIHN
Sbjct: 364 RQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHN 423

Query: 421 SQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAW-QSSKFAQATL 480
           S+I  A DL+P+VG+VKTY+GRPW K+SRTV++++Y+D +VSP GW  W + S F   TL
Sbjct: 424 SRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTL 483

Query: 481 YYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
           +Y EY+N GP +STR+RV W GFHV+   + AS F+V + IAG  WLP TG+PF  G+
Sbjct: 484 FYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of CSPI03G05560 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 565.1 bits (1455), Expect = 6.0e-161
Identity = 286/521 (54.89%), Postives = 372/521 (71.40%), Query Frame = 0


Query: 18  LSNNVSANPIIKWCKTTPHPRVC-----VSLMSPIK-PPPQNRREFRQMAIQTTLEKAAE 77
           ++N   +  +  WC  TPH   C      S  + I   PP+ R EF +M ++  L++A  
Sbjct: 75  INNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVI 134

Query: 78  ARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIHRRSCTDFDAQT 137
             + T +FGP+C  ++++ AW+DC  L+ + V QLNRTL   +   A     CTDFDAQT
Sbjct: 135 THSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLK-GLNPAASSDVKCTDFDAQT 194

Query: 138 WLSSALTDIDLCNSGAADLNVTDFITP-IKCLNVSKMISNCLAINGGFLEEEGVKYDDGR 197
           WLS+A T+I+ C SG+ DLNV+DF+ P I   N+S +I NCLA+NG  +++         
Sbjct: 195 WLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANH 254

Query: 198 NGSFPMWVSEGDRKLLESRP-GRVRANLVVAKDGSGTFRRVQAAIDAAARRRGRGRFIIY 257
              FP WVS  +R+LL S    +   +LVVA+D SG FR +QAAI+ AARRR + RF+IY
Sbjct: 255 KEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIY 314

Query: 258 VKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPGFIARD 317
           VK+GVYRENI+VGNDN NIMLVGDG R T+ITSGRSV  G+TT++SAT G  G  F+A+D
Sbjct: 315 VKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKD 374

Query: 318 IRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIF 377
           + F+NTAGP  GQAVA+RSSSDLSVF+R    G+QDTL + SQRQF+++CY+ GTIDFIF
Sbjct: 375 MTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIF 434

Query: 378 GNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADLRPMVGSVK 437
           GNAAVV QNCMI VRRPL GQ N+ITAQGR DPFQN+GI+IH+S+I AA+DL+P++ + K
Sbjct: 435 GNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYK 494

Query: 438 TYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAW-QSSKFAQATLYYGEYRNIGPRASTRFR 497
           TYLGRPW+ YSR  IM++YID  +SP GW  W + S FA  T++YGEY+N GP +STR+R
Sbjct: 495 TYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWR 554

Query: 498 VKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPFKLGV 530
           V+W GFH I S  VAS+F+V  LIAG +WLPATGVPFK G+
Sbjct: 555 VRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of CSPI03G05560 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 556.6 bits (1433), Expect = 2.1e-158
Identity = 293/525 (55.81%), Postives = 366/525 (69.71%), Query Frame = 0


Query: 7   ILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLM---SPIKPPPQNRREFRQMAIQ 66
           IL + +   T +++  S N I  WC  TP+P  C       S  + P Q   EFR + ++
Sbjct: 19  ILLLCLRPLTTVADGNSTN-IDGWCDKTPYPYPCKRYFIKHSGFRLPTQ-ISEFRVLLVE 78

Query: 67  TTLEKAAEARAYTARFGPTCKTSRQRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIHRRS 126
             +++A  A          C   +++    DC  LY D V+QLNRTL  V +      R 
Sbjct: 79  AAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTG---RR 138

Query: 127 CTDFDAQTWLSSALTDIDLCNSGAADLNVTDFITPI-KCLNVSKMISNCLAINGGFLEEE 186
           CTDFDAQTWLS+ALT+ + C  G++DLNV+DF TPI     +S +ISNCLA+NG  L   
Sbjct: 139 CTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAG 198

Query: 187 GVKYDDGRNGSFPMWVSEGDRKLLESRPGRVRANLVVAKDGSGTFRRVQAAIDAAARRR- 246
                 G +  FP WVS  +R+LL+ +   VRANLVVAKDGSG F+ VQAAID A RR+ 
Sbjct: 199 KNDSTTGDSKGFPTWVSRKERRLLQLQ--SVRANLVVAKDGSGHFKTVQAAIDVAGRRKV 258

Query: 247 GRGRFIIYVKRGVYRENIEVGNDNGNIMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQ 306
             GRF+IYVKRG+Y+EN+ V  +N NIMLVGDGMR+T+IT GRSV  G+TT+SSATAGI+
Sbjct: 259 TSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIE 318

Query: 307 GPGFIARDIRFVNTAGPRMGQAVALRSSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYV 366
           G  FIA+ I F NTAGP  GQAVALRSSSDLS+F+RCS EGYQDTLMV SQRQFY++CY+
Sbjct: 319 GLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYI 378

Query: 367 YGTIDFIFGNAAVVLQNCMIYVRRPLKGQVNVITAQGREDPFQNSGISIHNSQIRAAADL 426
           YGT+DFIFGNAAVV QNC+I  R PLKGQ NVITAQGR D FQN+GISIHNS I  A DL
Sbjct: 379 YGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDL 438

Query: 427 RPMVGSVKTYLGRPWKKYSRTVIMRSYIDWLVSPAGWLAW-QSSKFAQATLYYGEYRNIG 486
           +P+V SVKTY+GRPW  YSRTV++++YID +VSP GW  W + S +   TL+Y EY+NIG
Sbjct: 439 KPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIG 498

Query: 487 PRASTRFRVKWPGFHVIKSPNVASKFSVQRLIAGQTWLPATGVPF 526
           P +STR+RV+W GFHV+   + AS FSV + IAG  WLP +G+PF
Sbjct: 499 PASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

[Close]

BLAST of CSPI03G05560 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 485.7 bits (1249), Expect = 4.7e-137
Identity = 254/502 (50.60%), Postives = 335/502 (66.73%), Query Frame = 0


Query: 30  WCKTTPHPRVCVSLM--SPIKPPPQNRREFRQMAIQTTLEKAAEARAYTARFGPTCKTSR 89
           WC  TP+P+ C   +  +    P ++  EF +++++  L++A  A+ +    GP C+ +R
Sbjct: 33  WCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCRDTR 92

Query: 90  QRTAWTDCFKLYNDVVLQLNRTLHCVVTDEAIHRRSCTDFDAQTWLSSALTDIDLCNSGA 149
           ++ AW DC KLY+  V ++N T+   V         C+  DAQTWLS+ALT++D C +G 
Sbjct: 93  EKAAWEDCIKLYDLTVSKINETMDPNV--------KCSKLDAQTWLSTALTNLDTCRAGF 152

Query: 150 ADLNVTDFITPIKCLNVSKMISNCLAINGGFLEEEGVKYDDGRNGSFPMWVSEGDRKLLE 209
            +L VTD + P+   NVS ++ N LAIN     +    Y       FP WV  GDRKLL+
Sbjct: 153 LELGVTDIVLPLMSNNVSNLLCNTLAIN-----KVPFNYTPPEKDGFPSWVKPGDRKLLQ 212

Query: 210 SRPGRVRANLVVAKDGSGTFRRVQAAIDAAARRRGRGRFIIYVKRGVYRENIEVGNDNGN 269
           S     + N VVAKDGSG F+ ++ AIDAA+   G GRF+IYVK+GVY EN+E+     N
Sbjct: 213 S--STPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENLEIRKK--N 272

Query: 270 IMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALR 329
           +ML GDG+  T+IT  +SV  G TTF+SAT    G GFIAR I F NTAG    QAVALR
Sbjct: 273 VMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALR 332

Query: 330 SSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPL 389
           S SDLSVF++CSFE YQDTL V S RQFY+ C VYGT+DFIFGNAA VLQNC I+ RRP 
Sbjct: 333 SGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP- 392

Query: 390 KGQVNVITAQGREDPFQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRS 449
           + + N ITAQGR DP QN+GI IHNS++ AA+DLRP++GS KTYLGRPW++YSRTV M++
Sbjct: 393 RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKT 452

Query: 450 YIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFS 509
            +D L+ P GWL W  + FA  TL+Y E++N GP AST  RV WPGF V+ S + ASKF+
Sbjct: 453 SLDSLIDPRGWLEWDGN-FALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFT 511

Query: 510 VQRLIAGQTWLPATGVPFKLGV 530
           V   +AG +W+P++ VPF  G+
Sbjct: 513 VGTFLAGGSWIPSS-VPFTSGL 511

[Close]

BLAST of CSPI03G05560 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 389.8 bits (1000), Expect = 3.5e-108
Identity = 234/562 (41.64%), Postives = 320/562 (56.94%), Query Frame = 0


Query: 3   SHYKILFISITFFTLLSNNVSANPIIKWCKTTPHPRVCVSLM--SPIK--PPPQNRREFR 62
           S Y    I+I +   +S +++A+  I  CK TP+P VC   M  SP+K      +   F 
Sbjct: 12  SLYTKCIITIIYVVSIS-HLNAH-FITSCKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFH 71

Query: 63  QMAIQTTLEKAAEARAYTARFGPTCKTSRQRT-AWTDCFKLYNDVVLQLNRTLHCVVTDE 122
            + + +T+++A +     +         +  T A  DC +LY D + QLN +        
Sbjct: 72  DLVVSSTMDQAVQLHRLVSSLKQHHSLHKHATSALFDCLELYEDTIDQLNHSRRSYGQYS 131

Query: 123 AIHRRSCTDFDAQTWLSSALTDIDLCNSGAADLNVTDFIT---PIKC-LNVSKMISNCLA 182
           + H       D QT LS+A+ + D C +G  D  +T   +   P++   N++K ISN LA
Sbjct: 132 SPH-------DRQTSLSAAIANQDTCRNGFRDFKLTSSYSKYFPVQFHRNLTKSISNSLA 191

Query: 183 INGGFLEEEGV----------KYDDGRNGS---------------FPMWVSEGDRKLLES 242
           +     E E V          K+   R+ +               FP W    DRKLLE 
Sbjct: 192 VTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLED 251

Query: 243 RPGRVRANLVVAKDGSGTFRRVQAAIDAAAR-RRGRGRFIIYVKRGVYRENIEVGNDNGN 302
                +A+LVVAKDGSG +  +Q A++AAA+  R   R +IYVK GVYREN+ +     N
Sbjct: 252 SKTTAKADLVVAKDGSGHYTSIQQAVNAAAKLPRRNQRLVIYVKAGVYRENVVIKKSIKN 311

Query: 303 IMLVGDGMRFTVITSGRSVAAGFTTFSSATAGIQGPGFIARDIRFVNTAGPRMGQAVALR 362
           +M++GDG+  T++T  R+V  G TTF SAT  + G GFIA+ I F NTAGP   QAVALR
Sbjct: 312 VMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALR 371

Query: 363 SSSDLSVFHRCSFEGYQDTLMVLSQRQFYKQCYVYGTIDFIFGNAAVVLQNCMIYVRRPL 422
           SSSD SVF+ CSF+GYQDTL + S RQF + C +YGT+DFIFG+A  +LQNC IY R+P+
Sbjct: 372 SSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPM 431

Query: 423 KGQVNVITAQGREDPFQNSGISIHNSQIRAAADLRPMVGSVKTYLGRPWKKYSRTVIMRS 482
            GQ N ITAQ R++P + +G  I +S +  A++         TYLGRPW+ +SRTV M+ 
Sbjct: 432 SGQKNTITAQSRKEPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKC 491

Query: 483 YIDWLVSPAGWLAWQSSKFAQATLYYGEYRNIGPRASTRFRVKWPGFHVIKSPNVASKFS 530
            +  LVSPAGWL W S  FA +TLYYGEY N G  AS   RVKWPG+HVIK+   A KF+
Sbjct: 492 NLGALVSPAGWLPW-SGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFT 551

The following BLAST results are available for this feature:

BLAST of CSPI03G05560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHN5	8.2e-163	55.20	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	8.5e-160	54.89	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN4	3.0e-157	55.81	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
O04887	4.1e-138	49.34	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	6.6e-136	50.60	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CSPI03G05560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L330	1.4e-309	100.00	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G088980 PE=3 SV=1	[more]
A0A1S3BUR4	6.3e-291	94.56	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493398 PE=3 SV=1	[more]
A0A5A7TVK4	6.3e-267	94.66	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G001080...	[more]
A0A6J1EW59	5.8e-244	79.89	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438452 PE=3 SV=1	[more]
A0A6J1JCM7	7.5e-244	79.89	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483275 PE=3 SV=1	[more]

BLAST of CSPI03G05560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650542.1	2.8e-309	100.00	probable pectinesterase/pectinesterase inhibitor 59 [Cucumis sativus] >KGN56178....	[more]
XP_008452338.1	1.3e-290	94.56	PREDICTED: probable pectinesterase/pectinesterase inhibitor 59 [Cucumis melo] >X...	[more]
XP_038905752.1	5.9e-267	86.54	probable pectinesterase/pectinesterase inhibitor 60 [Benincasa hispida]	[more]
KAA0046994.1	1.3e-266	94.66	putative pectinesterase/pectinesterase inhibitor 59 [Cucumis melo var. makuwa] >...	[more]
XP_023520442.1	3.1e-244	79.89	probable pectinesterase/pectinesterase inhibitor 59 isoform X1 [Cucurbita pepo s...	[more]

BLAST of CSPI03G05560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51490.1	5.8e-164	55.20	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	6.0e-161	54.89	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	2.1e-158	55.81	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G45220.1	4.7e-137	50.60	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G23200.1	3.5e-108	41.64	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 23..174 e-value: 3.0E-25 score: 99.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 29..174 e-value: 3.3E-22 score: 79.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 8..175 e-value: 1.2E-20 score: 71.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 21..177 e-value: 1.7E-26 score: 95.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 27..176
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 216..514 e-value: 2.2E-127 score: 423.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 211..529 e-value: 7.7E-110 score: 369.2
None	No IPR available	PANTHER	PTHR31707:SF45	PECTINESTERASE/PECTINESTERASE INHIBITOR 59-RELATED	coord: 7..528
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 7..528
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 30..178 e-value: 6.04464E-33 score: 121.006
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 361..370
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 242..261
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 214..527

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G05560.1	CSPI03G05560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity