CSPI03G05330 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G05330
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Thiamine thiazole synthase, chloroplastic
Location	Chr3: 4332126 .. 4334327 (-)
RNA-Seq Expression	CSPI03G05330
Synteny	CSPI03G05330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAGCGTGTAAGATATTGTTACATTCTTCAATATCCTCTATAAAACCCTTCTTCCTTCCCCTCTTCTTCCCCACACCAAACTACAATCTCCTCTGTTTTCCTCTGCTTCAAAAATGGCTTCCATTGCCTCCACTCTCACCACCAAGCTCCAAAAACCTTCTTTACTTCATGATTCCTCTTTCCATGGCACACCTCTCGCCTCTCCTTCTTCTCTCCGCCTCAAATCCACCGCCGCCACGTCCCTCACCATCTCTGCCTCCGCCTCTCAGCCTCCCTACGATTTAAACCAATTCAAATTCAACCCTATCAGAGAATCTATTGTCTCTCGAGAGATGACCCGTCGATACATGACCGATATGATCACCTACGCTGATACTGACGTTGTCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAACTCAGCAAAAACCCCAATATTCGTATTGCCATCATCGAACAATCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTAACAAGTTCACTTTCCAAATTTGAATGGATGCTCTGTTTTTATTTTATGTTTTGTTCTTTTCTTGAAAATACCAAAATTTTGAAAATCACCCAATCAGCTTTCATGCATGCCGTATTTGGACTAAAAAAAATCATTTCTTTTTAATAGAGATCAGAAATAAAGAAACCCAGAAACTAATATCATAATCATTAATTTGAGGGGAAATAGTTTACAGATCTTGTGATCCCCTAAAAAATTAGATTAATTTTCATCGCAGACAAATATTCTGATCTTGTTCATCTGATTGATGTTGGTTAAATTTAATGTTTGATAGGTGGTTCGTAAGCCAGCTCATTTGTTCTTGGATGAGGTGGGTGTGGAGTATGACGAGCAAGACGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATTGTCAAGGGCGGCAGAGTTGGCGGTGTTGTCACCAACTGGGCTCTTGTGTCAATGAACCATGACACGCAGTCCTGCATGGACCCCAATGTGATGGAGGCTAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTTGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCATGATTGATAGTGTCCCGGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCGGAGATCGATGGAGCTCCAAGAATGGTAAACAAATTAACTAGAGAGAGATTATTGCTTTAGACCGTTTAATAATCATGATACAGTAAAATATAGCAGCATTTAGATGAGTTTACACTTTTCAGAGTTTATCTGGGTCTATCACTACTAACTTGTTTACTCCGGTAGCTATCTGCATTAGATGCTACCACCGAAAGATTTCGTTATACCCATATTTATTAGTCTTAAACTGTGCCATTCATAGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCAGCACACTTGGCACTAAAGTCATTGGGAGAGGCGAACAGCATTGGGGATGAAGGAGAGATGGGAGAAAGAAAGGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCGGCGGGAGAGTCACCGGAAGTTGCAGATGCTTAAAGTGTTTAGTAGCCGGCGGATGTTGATTTGGAAGAAATATTTGCTTTTGGGTTTTATGGTGCTGTTTGTTTGTTTGTAGTGAGTTTTCAGTAAACGAAAAGGGGCGATGGTTGTGTTTGGCTTTTTTGTGTTTTCATAACACAATAGGGTTTGGGGAAAGACAAAAAATATCAGTTGCCTTTTTCTCCCTCTCTTCTTTTTTTTATTTTGTTAATAAAAATGCCTTTCCAAAATATCTCTCCTCCCCACCCCATCCAAATTGGTAATGTTGGTCACGATTAACCCAACAGGAAACGTAATCAATCTATGATAATGTGATTGATGTACACTACTCTTCCTCTGTGGCTCTGCCATCCAATGTTAAACTATTGTGATTCCTAAAATTTCATTATGGGTTTAACTTGAACTCTTAAAACTTCATTACGATCCAAGGTGATAAGAGTCTCTCATAAATCATAATTATATTTGGAACGAGG

mRNA sequence

CAAGCGTGTAAGATATTGTTACATTCTTCAATATCCTCTATAAAACCCTTCTTCCTTCCCCTCTTCTTCCCCACACCAAACTACAATCTCCTCTGTTTTCCTCTGCTTCAAAAATGGCTTCCATTGCCTCCACTCTCACCACCAAGCTCCAAAAACCTTCTTTACTTCATGATTCCTCTTTCCATGGCACACCTCTCGCCTCTCCTTCTTCTCTCCGCCTCAAATCCACCGCCGCCACGTCCCTCACCATCTCTGCCTCCGCCTCTCAGCCTCCCTACGATTTAAACCAATTCAAATTCAACCCTATCAGAGAATCTATTGTCTCTCGAGAGATGACCCGTCGATACATGACCGATATGATCACCTACGCTGATACTGACGTTGTCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAACTCAGCAAAAACCCCAATATTCGTATTGCCATCATCGAACAATCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTTCGTAAGCCAGCTCATTTGTTCTTGGATGAGGTGGGTGTGGAGTATGACGAGCAAGACGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATTGTCAAGGGCGGCAGAGTTGGCGGTGTTGTCACCAACTGGGCTCTTGTGTCAATGAACCATGACACGCAGTCCTGCATGGACCCCAATGTGATGGAGGCTAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTTGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCATGATTGATAGTGTCCCGGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCGGAGATCGATGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCAGCACACTTGGCACTAAAGTCATTGGGAGAGGCGAACAGCATTGGGGATGAAGGAGAGATGGGAGAAAGAAAGGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCGGCGGGAGAGTCACCGGAAGTTGCAGATGCTTAAAGTGTTTAGTAGCCGGCGGATGTTGATTTGGAAGAAATATTTGCTTTTGGGTTTTATGGTGCTGTTTGTTTGTTTGTAGTGAGTTTTCAGTAAACGAAAAGGGGCGATGGTTGTGTTTGGCTTTTTTGTGTTTTCATAACACAATAGGGTTTGGGGAAAGACAAAAAATATCAGTTGCCTTTTTCTCCCTCTCTTCTTTTTTTTATTTTGTTAATAAAAATGCCTTTCCAAAATATCTCTCCTCCCCACCCCATCCAAATTGGTAATGTTGGTCACGATTAACCCAACAGGAAACGTAATCAATCTATGATAATGTGATTGATGTACACTACTCTTCCTCTGTGGCTCTGCCATCCAATGTTAAACTATTGTGATTCCTAAAATTTCATTATGGGTTTAACTTGAACTCTTAAAACTTCATTACGATCCAAGGTGATAAGAGTCTCTCATAAATCATAATTATATTTGGAACGAGG

Coding sequence (CDS)

ATGGCTTCCATTGCCTCCACTCTCACCACCAAGCTCCAAAAACCTTCTTTACTTCATGATTCCTCTTTCCATGGCACACCTCTCGCCTCTCCTTCTTCTCTCCGCCTCAAATCCACCGCCGCCACGTCCCTCACCATCTCTGCCTCCGCCTCTCAGCCTCCCTACGATTTAAACCAATTCAAATTCAACCCTATCAGAGAATCTATTGTCTCTCGAGAGATGACCCGTCGATACATGACCGATATGATCACCTACGCTGATACTGACGTTGTCATCGTCGGCGCTGGCTCCGCCGGCCTCTCTTGCGCTTACGAACTCAGCAAAAACCCCAATATTCGTATTGCCATCATCGAACAATCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGCGGCCAACTCTTCTCTGCTATGGTGGTTCGTAAGCCAGCTCATTTGTTCTTGGATGAGGTGGGTGTGGAGTATGACGAGCAAGACGACTATGTCGTGATAAAGCACGCGGCTCTGTTCACGTCGACGATCATGAGCAAGCTCTTGGCCCGGCCGAACGTGAAGCTGTTCAACGCCGTGGCGGCAGAGGATTTGATTGTCAAGGGCGGCAGAGTTGGCGGTGTTGTCACCAACTGGGCTCTTGTGTCAATGAACCATGACACGCAGTCCTGCATGGACCCCAATGTGATGGAGGCTAAGGTGGTTGTGAGCTCTTGTGGCCACGATGGACCTTTTGGCGCTACCGGAGTCAAGCGCCTCAAGAGCATCGGCATGATTGATAGTGTCCCGGGAATGAAGGCCCTTGACATGAACACTGCTGAGGATGCCATTGTTCGATTCACTCGAGAGGTCGTTCCTGGCATGATTGTCACCGGCATGGAGGTTGCGGAGATCGATGGAGCTCCAAGAATGGGGCCAACGTTCGGGGCGATGATGATATCAGGGCAGAAGGCAGCACACTTGGCACTAAAGTCATTGGGAGAGGCGAACAGCATTGGGGATGAAGGAGAGATGGGAGAAAGAAAGGTTGGACTTGAAGAGGAAGAGTTGCTACTGGCGGCGGGAGAGTCACCGGAAGTTGCAGATGCTTAA

Protein sequence

MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA*

Homology

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BLAST of CSPI03G05330 vs. ExPASy Swiss-Prot
Match: O23787 (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 4.1e-158
Identity = 298/364 (81.87%), Postives = 322/364 (88.46%), Query Frame = 0


Query: 1   MASIAST--LTTKLQKPSLLHDSSFHGTPLASPSSLRL---KSTAATSLTISASASQPPY 60
           MA++AST    +     + L DSSFHG P+ SPS LRL   KS+   +L+ISASAS PPY
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPM-SPSLLRLQPIKSSRPNNLSISASAS-PPY 60

Query: 61  DLNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIA 120
           DLN FKF+PI+ESIVSREMTRRYMTDMITYADTDVV+VGAGSAGLSCAYELSKNPNI+IA
Sbjct: 61  DLNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH+FLDE+G++YDEQD+YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRLKSIGMI+ VPGMKALDMN+AEDAIVR TREVVPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPE 360
           GAPRMGPTFGAMMISGQKAAHLALKSLG+ N++          VG    EL+LAA +S E
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDG------TYVGGVHPELILAAADSAE 356

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BLAST of CSPI03G05330 vs. ExPASy Swiss-Prot
Match: Q38709 (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 4.9e-151
Identity = 285/360 (79.17%), Postives = 315/360 (87.50%), Query Frame = 0


Query: 4   IASTLTTKLQKPSLLHD--SSFHGTPLASPSSLRLKST-AATSLTISAS-ASQPPYDLNQ 63
           +ASTLT+K Q+ +L  +  SSF+GTPLA PSS+R++ T A    +IS S A  PPYDL  
Sbjct: 1   MASTLTSKPQRLALFENSASSFYGTPLA-PSSIRVQPTKAGAKPSISMSGAPSPPYDLKA 60

Query: 64  FKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQ 123
           F F+PI+ESIVSREMTRRYM DMITYADTDVV+VGAGS+GL C YELSKNP++++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIEQ 120

Query: 124 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 183
           SVSPGGGAWLGGQLFS MVVRKPAHLFLDE+G+EYDEQD+YVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLLA 180

Query: 184 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 243
           RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 244 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 303
           PFGATGVK L+SIGMID+VPGMKALDMN AEDAIVR TRE+VPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAPR 300

Query: 304 MGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALK+LG  N++          VG    EL+LAA +S E+ADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGLPNALDGS------YVGGIHPELILAAADSAEIADA 352

[Close]

BLAST of CSPI03G05330 vs. ExPASy Swiss-Prot
Match: F6H7K5 (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 9.2e-150
Identity = 287/362 (79.28%), Postives = 313/362 (86.46%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPS---LLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDL 60
           MAS+A+TLT+    P      + SSFHG+P+ S   L +K  +  S TIS S S  PYDL
Sbjct: 1   MASMATTLTSLSSNPKPAFFDNKSSFHGSPI-SYRVLPIK-VSHQSPTISMS-SVSPYDL 60

Query: 61  NQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAII 120
             FKF PI+ESIV+REMTRRYM DMITYADTDVVIVGAGSAGLSCAYELSKNP+IR+AII
Sbjct: 61  QSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRVAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIMSKL 180

Query: 181 LARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LARPNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240

Query: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TRE+VPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVA 360
           PRMGPTFGAMMISGQKAAHLAL++LG+ N+I  +G   E +    + EL+LAA E+ E+ 
Sbjct: 301 PRMGPTFGAMMISGQKAAHLALRALGQPNAI--DGNYTEAET--MQPELILAAAETGEIV 355

[Close]

BLAST of CSPI03G05330 vs. ExPASy Swiss-Prot
Match: F6H9A9 (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 1.6e-149
Identity = 275/361 (76.18%), Postives = 312/361 (86.43%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLL--HDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLN 60
           MA++ S++ +K  K S+   H SSFHG P+A+ +  RL    +T + ++ +A+  PYDL 
Sbjct: 1   MATLTSSICSK-PKASVFDPHKSSFHGVPIATQA--RLSPVKSTPVNLAVTAAAMPYDLR 60

Query: 61  QFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIE 120
            FKF PI+ESIVSREMTRRYM DMITYADTDVV+VGAGSAGLSCAYELSKNP++++AIIE
Sbjct: 61  SFKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVAIIE 120

Query: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180
           QSVSPGGGAWLGGQLFS+MVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTIMSKLL 180

Query: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240
           ARPNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 241 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 300
           GPFGATGVKRL+S+GMIDSVPGMKALDMNTAED IVR TREVVPGMIVTGMEVAEIDG+P
Sbjct: 241 GPFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDGSP 300

Query: 301 RMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVAD 360
           RMGPTFGAMMISGQKAAHLALKSLG  N++      G     L  E +L AA ++ E+A+
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGLPNALD-----GTYIGNLHPELVLAAAADAAEIAE 353

[Close]

BLAST of CSPI03G05330 vs. ExPASy Swiss-Prot
Match: Q38814 (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI1 PE=1 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 1.6e-149
Identity = 280/360 (77.78%), Postives = 307/360 (85.28%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPL-ASPSSLRLKSTAATSLTISASASQPPYDLNQ 60
           MA+IASTL+    KP  L DSSFHG+ + A+P S+ LK       + S  A+   YDLN 
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPR-----SFSVRATTAGYDLNA 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           F F+PI+ESIVSREMTRRYMTDMITYA+TDVV+VGAGSAGLS AYE+SKNPN+++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQD YVV+KHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIGMID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKA  LALK+LG  N+I  +G +    VG    EL+LAA +S E  DA
Sbjct: 301 MGPTFGAMMISGQKAGQLALKALGLPNAI--DGTL----VGNLSPELVLAAADSAETVDA 349

[Close]

BLAST of CSPI03G05330 vs. ExPASy TrEMBL
Match: A0A0A0L546 (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 8.0e-197
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of CSPI03G05330 vs. ExPASy TrEMBL
Match: A0A1S3CJ81 (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.4e-193
Identity = 353/359 (98.33%), Postives = 355/359 (98.89%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQ PSLLHDSSFHGTPLASP SLRLKS AATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIG EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of CSPI03G05330 vs. ExPASy TrEMBL
Match: A0A6J1J7L4 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483293 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.2e-171
Identity = 319/360 (88.61%), Postives = 340/360 (94.44%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLK-STAATSLTISASASQPPYDLNQ 60
           MASIAS+L TKL +PSLL DSSFHG+PL    S RLK +TAA SL+ISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPTKLHRPSLL-DSSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPIRESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALK+LG+ANSI ++GE G RKV  E  E++LAAGE+PEVADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGRKV--EAAEMVLAAGETPEVADA 357

[Close]

BLAST of CSPI03G05330 vs. ExPASy TrEMBL
Match: A0A6J1FWL6 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448035 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.9e-166
Identity = 320/360 (88.89%), Postives = 328/360 (91.11%), Query Frame = 0


Query: 1   MASIASTLTTKLQKP-SLLHDSSFHGTPLA-SPSSLRLKSTAATSLTISASASQPPYDLN 60
           MAS+ASTLTTKLQ P S L DSSFHGTPLA  P SLRLKST A + +ISAS  Q PYDLN
Sbjct: 1   MASVASTLTTKLQTPSSSLLDSSFHGTPLAPPPPSLRLKSTPA-AFSISAS-QQQPYDLN 60

Query: 61  QFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIE 120
            FKFNPI+ESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNP+IRIAIIE
Sbjct: 61  SFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIE 120

Query: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLL 180

Query: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240
           ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 241 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 300
           GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 300

Query: 301 RMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVAD 359
           RMGPTFGAMMISGQKAAHLALKSLG AN I DE             +L+LAAGESPEVAD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA-----------ADLILAAGESPEVAD 347

[Close]

BLAST of CSPI03G05330 vs. ExPASy TrEMBL
Match: A0A6J1EVU2 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438464 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.3e-166
Identity = 310/360 (86.11%), Postives = 332/360 (92.22%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLK-STAATSLTISASASQPPYDLNQ 60
           MASIAS+L  KL +PSLL +SSFHG+PL    S RLK +TAA SL+ISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPIKLHRPSLL-ESSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPIRESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIGMIDSVPGMKALDMN+AEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNSAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALK+LG+ NSI  E         +E  E++LAAGE+PEVADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKTNSIDGE---------VEAAEMVLAAGETPEVADA 350

[Close]

BLAST of CSPI03G05330 vs. NCBI nr
Match: XP_004152916.1 (thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothetical protein Csa_011133 [Cucumis sativus])

HSP 1 Score: 696.0 bits (1795), Expect = 1.7e-196
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of CSPI03G05330 vs. NCBI nr
Match: XP_008463440.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 684.5 bits (1765), Expect = 5.0e-193
Identity = 353/359 (98.33%), Postives = 355/359 (98.89%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 60
           MASIASTLTTKLQ PSLLHDSSFHGTPLASP SLRLKS AATSLTISASASQPPYDLNQF
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 60

Query: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120
           KFNPIRESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNPNIRIAIIEQS
Sbjct: 61  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 120

Query: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180
           VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR
Sbjct: 121 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 180

Query: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 181 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 240

Query: 241 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 241 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 300

Query: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           GPTFGAMMISGQKAAHLALKSLGEANSIG EGEMGERKVGLEEEELLLAAGESPEVADA
Sbjct: 301 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of CSPI03G05330 vs. NCBI nr
Match: XP_038905461.1 (thiamine thiazole synthase, chloroplastic [Benincasa hispida])

HSP 1 Score: 647.9 bits (1670), Expect = 5.2e-182
Identity = 340/363 (93.66%), Postives = 352/363 (96.97%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKS----TAATSLTISASASQPPYD 60
           MASIASTLTTKLQ+PSLL DSSF+GTPLA P SLRLKS    TAATSL+ISASASQPPYD
Sbjct: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSLRLKSTTTTTAATSLSISASASQPPYD 60

Query: 61  LNQFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAI 120
           LNQFKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSKNP+IRIAI
Sbjct: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEV 360
           APRMGPTFGAMMISGQKAAHLALKSLGEAN I ++GE+GERKV +EE ELLLAAGESPEV
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGI-EDGEVGERKV-VEEPELLLAAGESPEV 360

[Close]

BLAST of CSPI03G05330 vs. NCBI nr
Match: XP_022985241.1 (thiamine thiazole synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 612.5 bits (1578), Expect = 2.4e-171
Identity = 319/360 (88.61%), Postives = 340/360 (94.44%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLK-STAATSLTISASASQPPYDLNQ 60
           MASIAS+L TKL +PSLL DSSFHG+PL    S RLK +TAA SL+ISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPTKLHRPSLL-DSSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPIRESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALK+LG+ANSI ++GE G RKV  E  E++LAAGE+PEVADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGRKV--EAAEMVLAAGETPEVADA 357

[Close]

BLAST of CSPI03G05330 vs. NCBI nr
Match: XP_023520449.1 (thiamine thiazole synthase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 606.3 bits (1562), Expect = 1.7e-169
Identity = 314/360 (87.22%), Postives = 340/360 (94.44%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLK-STAATSLTISASASQPPYDLNQ 60
           MASIAS+L TKL +PSLL +SSFHG+PL    S RLK +TAA SL+ISASA+QPPYDLNQ
Sbjct: 1   MASIASSLPTKLHRPSLL-ESSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           FKFNPIRESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSK+P++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKLL+
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLS 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIGMIDSVPGMKALDMN+AEDAIVRFTREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNSAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKAAHLALK+LG+ANSI ++GE G R+V  E  E++LAAGE+PEVADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGREV--EAAEMVLAAGETPEVADA 357

[Close]

BLAST of CSPI03G05330 vs. TAIR 10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) )

HSP 1 Score: 530.4 bits (1365), Expect = 1.1e-150
Identity = 280/360 (77.78%), Postives = 307/360 (85.28%), Query Frame = 0


Query: 1   MASIASTLTTKLQKPSLLHDSSFHGTPL-ASPSSLRLKSTAATSLTISASASQPPYDLNQ 60
           MA+IASTL+    KP  L DSSFHG+ + A+P S+ LK       + S  A+   YDLN 
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPR-----SFSVRATTAGYDLNA 60

Query: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQ 120
           F F+PI+ESIVSREMTRRYMTDMITYA+TDVV+VGAGSAGLS AYE+SKNPN+++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAM+VRKPAHLFLDE+GV YDEQD YVV+KHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLA 180

Query: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           RPNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDG 240

Query: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRLKSIGMID VPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 360
           MGPTFGAMMISGQKA  LALK+LG  N+I  +G +    VG    EL+LAA +S E  DA
Sbjct: 301 MGPTFGAMMISGQKAGQLALKALGLPNAI--DGTL----VGNLSPELVLAAADSAETVDA 349

The following BLAST results are available for this feature:

BLAST of CSPI03G05330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23787	4.1e-158	81.87	Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=...	[more]
Q38709	4.9e-151	79.17	Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=...	[more]
F6H7K5	9.2e-150	79.28	Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2...	[more]
F6H9A9	1.6e-149	76.18	Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1...	[more]
Q38814	1.6e-149	77.78	Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI...	[more]

BLAST of CSPI03G05330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L546	8.0e-197	100.00	Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=...	[more]
A0A1S3CJ81	2.4e-193	98.33	Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160...	[more]
A0A6J1J7L4	1.2e-171	88.61	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1FWL6	1.9e-166	88.89	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1EVU2	4.3e-166	86.11	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of CSPI03G05330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152916.1	1.7e-196	100.00	thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothet...	[more]
XP_008463440.1	5.0e-193	98.33	PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo]	[more]
XP_038905461.1	5.2e-182	93.66	thiamine thiazole synthase, chloroplastic [Benincasa hispida]	[more]
XP_022985241.1	2.4e-171	88.61	thiamine thiazole synthase, chloroplastic-like [Cucurbita maxima]	[more]
XP_023520449.1	1.7e-169	87.22	thiamine thiazole synthase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI03G05330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54770.1	1.1e-150	77.78	thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00411	PNDRDTASEI	coord: 89..111 score: 30.78 coord: 344..358 score: 28.63
None	No IPR available	PFAM	PF01946	Thi4	coord: 71..304 e-value: 6.8E-115 score: 382.2
None	No IPR available	GENE3D	6.10.250.2840		coord: 56..86 e-value: 4.6E-19 score: 69.8
None	No IPR available	PANTHER	PTHR43422	THIAMINE THIAZOLE SYNTHASE	coord: 1..359
None	No IPR available	PANTHER	PTHR43422:SF11	THIAMINE THIAZOLE SYNTHASE, CHLOROPLASTIC	coord: 1..359
IPR002922	Thiazole biosynthetic enzyme Thi4 family	TIGRFAM	TIGR00292	TIGR00292	coord: 67..322 e-value: 2.2E-82 score: 274.1
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 87..322 e-value: 2.7E-122 score: 408.5
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 47..316
IPR027495	Thiamine thiazole synthase	HAMAP	MF_03158	THI4	coord: 5..331 score: 68.747131

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G05330.1	CSPI03G05330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0052837	thiazole biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005829	cytosol
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0016763	pentosyltransferase activity