CSPI03G04500 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G04500
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Pectin lyase-like superfamily protein, putative
Location	Chr3: 3656113 .. 3657863 (-)
RNA-Seq Expression	CSPI03G04500
Synteny	CSPI03G04500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCTCAGTTTTCAAATCTATACATTCAATGGTTATTTGCAAGTACAATTAATTCATAATACGTATTATTACTCTATTATTAGTTGTTCTTTTTCACCAAACTTACACAAAAGCCTTCTTAATATTGGACTTGTAATATACAAAATAAATATAAATCAAAATATGATCCCATACGTCTCTATATATTTGGAGAATTATTATAAAAAGTAAAATTGTCCAAAATATTTATAAATAAATTCTATCACAATGGTATTAGTTGATTTGTTTGTATAAATATAGGCATTAAGCAGTGCATGGAGTGGAGCTTGTGGAAATAATGGAGGTGGGGTTGTTTTAATACCAGCACAAGGAAAGTTCTTGGTTTTGCCATTGTTGCTTCAAGGTCCATGCCATGGCGTCATTCGTATACAGCTTGATGGCGAACTGTTGGCTTCCTCTGATAAGCAATTTGCCATCGGCGACCATTGGCTTAGTATCGAAAATGTCAACAACTTGTTCATCGACGGTTTGGGTCGCTTGACCGGCAGTGGTGCTTCTGCTTGGCCTTCCAACTCTCTTAACCGTCCCATCGTAAGTTCCATCTTTCTGTTGCATTTTTCACTAACAGTTTACCTAATATATTACATTTACTTTTGTTTCTATAGAGCATTCTATCTCAAATTTCTATTCAACTCTATAATTTTAGAGAATTATTGCTCTCAATACTTTGGTTTTGGGTTTGAGACTTTGAGAGTTGGTTTTCTTTATTTGGAAAAAAGTCCGTATTTGTTTTTGTTGACTTTAAACTAGATTATGCGATCATCTCTCACATTTATTTTAAATGCAATAGATAACGGTTTGATATTCTATGTAGTCTATGAAATTCAATAATGTCAATACTGCAAGTATCAAGAACATAAGCTCCTTCGACAGCAAATTCTTCCATTTTGCTGTGCATGGATCTCGTGATGTGACGTTTGATAACGTTACAGTGGTAGCTCCAGCAAACAGTCCCAACACAGATGGAATCCACATTTCAACGTCAAGTGGTATAAACATCATGCATTCGACAATCGGTACTGGAGATGATTGCATCTCATTGGGTCCTGGAAGCAAAATGATCAACATAAGTAACGTTCATTGTGGTCCTGGTCATGGAATTAGCATTGGTAGCCTTGGAAAGAACCCAAACGAAGAAGACGTCTTTGATGTCACAGTAAGAGACTCCACATTCATTGGCACATCCAACGGTGCGAGGATCAAATCATGGTCGTCTCCGTATTCCAGTATGGTTAGCAAGGTCACATTTCTCAATCTCCAAATGAACAATGTCAAAAACCCTATCATCATCGATCAAAGTTATTGTCCAGACTCTTGCGCTCCAAATAAAATGGTATAGTGTATAAGATAATTTAAGAGCATTAGTGTTATATATATAGCAAATTACAACGAATGTATACATATGAATTCTAAAGCAAAAGATTATGGTTTTTGTGGTTGGCAGAATAAATCAATGGTGCAGATTAAAGATGTGAGATATCAAGGCATAACGGGGAGTTCAAATACACCAGTGGCAGTAGATTTTGAATGCAGCCAAGTTCTTCCATGTCAAGGCATAGTGTTACAAGACATTAACCTTACTTTCAATGGTGGTGGGAAGACAACTTCCATTTGTCATAATGTTAAGGGTTCTGCTTCTGGTCAACAACTACCTCCCTCTTGCCTTTAATTACTTTGTGTTCTTTTCTTTTCTTCCTCACTACAGAGAG

mRNA sequence

ATGGGTCTCAGTTTTCAAATCTATACATTCAATGGTTATTTGCAAGCATTAAGCAGTGCATGGAGTGGAGCTTGTGGAAATAATGGAGGTGGGGTTGTTTTAATACCAGCACAAGGAAAGTTCTTGGTTTTGCCATTGTTGCTTCAAGGTCCATGCCATGGCGTCATTCGTATACAGCTTGATGGCGAACTGTTGGCTTCCTCTGATAAGCAATTTGCCATCGGCGACCATTGGCTTAGTATCGAAAATGTCAACAACTTGTTCATCGACGGTTTGGGTCGCTTGACCGGCAGTGGTGCTTCTGCTTGGCCTTCCAACTCTCTTAACCGTCCCATCTCTATGAAATTCAATAATGTCAATACTGCAAGTATCAAGAACATAAGCTCCTTCGACAGCAAATTCTTCCATTTTGCTGTGCATGGATCTCGTGATGTGACGTTTGATAACGTTACAGTGGTAGCTCCAGCAAACAGTCCCAACACAGATGGAATCCACATTTCAACGTCAAGTGGTATAAACATCATGCATTCGACAATCGGTACTGGAGATGATTGCATCTCATTGGGTCCTGGAAGCAAAATGATCAACATAAGTAACGTTCATTGTGGTCCTGGTCATGGAATTAGCATTGGTAGCCTTGGAAAGAACCCAAACGAAGAAGACGTCTTTGATGTCACAGTAAGAGACTCCACATTCATTGGCACATCCAACGGTGCGAGGATCAAATCATGGTCGTCTCCGTATTCCAGTATGGTTAGCAAGGTCACATTTCTCAATCTCCAAATGAACAATGTCAAAAACCCTATCATCATCGATCAAAGTTATTGTCCAGACTCTTGCGCTCCAAATAAAATGAATAAATCAATGGTGCAGATTAAAGATGTGAGATATCAAGGCATAACGGGGAGTTCAAATACACCAGTGGCAGTAGATTTTGAATGCAGCCAAGTTCTTCCATGTCAAGGCATAGTGTTACAAGACATTAACCTTACTTTCAATGGTGGTGGGAAGACAACTTCCATTTGTCATAATGTTAAGGGTTCTGCTTCTGGTCAACAACTACCTCCCTCTTGCCTTTAATTACTTTGTGTTCTTTTCTTTTCTTCCTCACTACAGAGAG

Coding sequence (CDS)

ATGGGTCTCAGTTTTCAAATCTATACATTCAATGGTTATTTGCAAGCATTAAGCAGTGCATGGAGTGGAGCTTGTGGAAATAATGGAGGTGGGGTTGTTTTAATACCAGCACAAGGAAAGTTCTTGGTTTTGCCATTGTTGCTTCAAGGTCCATGCCATGGCGTCATTCGTATACAGCTTGATGGCGAACTGTTGGCTTCCTCTGATAAGCAATTTGCCATCGGCGACCATTGGCTTAGTATCGAAAATGTCAACAACTTGTTCATCGACGGTTTGGGTCGCTTGACCGGCAGTGGTGCTTCTGCTTGGCCTTCCAACTCTCTTAACCGTCCCATCTCTATGAAATTCAATAATGTCAATACTGCAAGTATCAAGAACATAAGCTCCTTCGACAGCAAATTCTTCCATTTTGCTGTGCATGGATCTCGTGATGTGACGTTTGATAACGTTACAGTGGTAGCTCCAGCAAACAGTCCCAACACAGATGGAATCCACATTTCAACGTCAAGTGGTATAAACATCATGCATTCGACAATCGGTACTGGAGATGATTGCATCTCATTGGGTCCTGGAAGCAAAATGATCAACATAAGTAACGTTCATTGTGGTCCTGGTCATGGAATTAGCATTGGTAGCCTTGGAAAGAACCCAAACGAAGAAGACGTCTTTGATGTCACAGTAAGAGACTCCACATTCATTGGCACATCCAACGGTGCGAGGATCAAATCATGGTCGTCTCCGTATTCCAGTATGGTTAGCAAGGTCACATTTCTCAATCTCCAAATGAACAATGTCAAAAACCCTATCATCATCGATCAAAGTTATTGTCCAGACTCTTGCGCTCCAAATAAAATGAATAAATCAATGGTGCAGATTAAAGATGTGAGATATCAAGGCATAACGGGGAGTTCAAATACACCAGTGGCAGTAGATTTTGAATGCAGCCAAGTTCTTCCATGTCAAGGCATAGTGTTACAAGACATTAACCTTACTTTCAATGGTGGTGGGAAGACAACTTCCATTTGTCATAATGTTAAGGGTTCTGCTTCTGGTCAACAACTACCTCCCTCTTGCCTTTAA

Protein sequence

MGLSFQIYTFNGYLQALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIGDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMVSKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL*

Homology

[Close]

BLAST of CSPI03G04500 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.2e-79
Identity = 153/348 (43.97%), Postives = 221/348 (63.51%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSD-KQFA 74
           +A+  AW+ ACG  G   +LIP +G FLV PL   GPC G + IQ++G LLA++D  Q+ 
Sbjct: 58  KAVQEAWASACGGTGKQTILIP-KGDFLVGPLNFTGPCKGDVTIQVNGNLLATTDLSQYK 117

Query: 75  IGDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNR-------PISMKFNNVNTASIKN 134
              +W+ I  V+NL I G G+L G G + W  NS  +       P S+  + VN   +  
Sbjct: 118 DHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCKILPNSLVMDFVNNGEVSG 177

Query: 135 ISSFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCI 194
           I+  +SKFFH  ++  +D+   +V V AP +SPNTDGIH+  SSG+ I ++ IG GDDCI
Sbjct: 178 ITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGVTITNTVIGVGDDCI 237

Query: 195 SLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSS 254
           S+GPG+  +NI+ V CGPGHGISIGSLG+  +E+DV D+ V+D T   T+NG RIK++  
Sbjct: 238 SIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTANGVRIKAYED 297

Query: 255 PYSSM-VSKVTFLNLQMNNVKNPIIIDQSYCPDS-CAPNKMNKSMVQIKDVRYQGITGSS 314
             S +  SK+ + N++M +   PIIID  YCP+  C  N  +K  V +KDV ++ ITG+S
Sbjct: 298 AASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANGASK--VTVKDVTFKNITGTS 357

Query: 315 NTPVAVDFECSQVLPCQGIVLQDINLTFNG-GGKTTSICHNVKGSASG 352
           +TP AV+  C+  +PC G+ + D+N+ ++G   KT ++C N KGSA G
Sbjct: 358 STPEAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKGSAKG 402

[Close]

BLAST of CSPI03G04500 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 154/348 (44.25%), Postives = 217/348 (62.36%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSD-KQFA 74
           +A+  AW+ ACG  G   +LIP +G FLV  L   GPC G + IQ+DG LLA++D  Q+ 
Sbjct: 58  KAVQEAWASACGGTGKQTILIP-KGDFLVGQLNFTGPCKGDVTIQVDGNLLATTDLSQYK 117

Query: 75  IGDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNR-------PISMKFNNVNTASIKN 134
              +W+ I  V+NL I G G L G G + W  NS  +       P S+  + VN   +  
Sbjct: 118 DHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSG 177

Query: 135 ISSFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCI 194
           ++  +SKFFH  ++  +D+   +VTV AP +SPNTDGIH+  SSGI I ++ IG GDDCI
Sbjct: 178 VTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCI 237

Query: 195 SLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSS 254
           S+GPG+  +NI+ V CGPGHGISIGSLG+  +E+DV D+ V+D T   T  G RIK++  
Sbjct: 238 SIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYED 297

Query: 255 PYSSM-VSKVTFLNLQMNNVKNPIIIDQSYCPDS-CAPNKMNKSMVQIKDVRYQGITGSS 314
             S + VSK+ + N++M +  NPI ID  YCP+  C  N  +K  V +KDV ++ ITG+S
Sbjct: 298 AASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGASK--VTVKDVTFKNITGTS 357

Query: 315 NTPVAVDFECSQVLPCQGIVLQDINLTFNG-GGKTTSICHNVKGSASG 352
           +TP AV   C+  +PC G+ + D+N+ ++G   KT +IC N KGS  G
Sbjct: 358 STPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNAKGSTKG 402

[Close]

BLAST of CSPI03G04500 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.6e-77
Identity = 152/348 (43.68%), Postives = 216/348 (62.07%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSD-KQFA 74
           +A+  AW+ ACG  G   +LIP +G FLV  L   GPC G + IQ+DG LLA++D  Q+ 
Sbjct: 58  KAVQEAWASACGGTGKQTILIP-KGDFLVGQLNFTGPCKGDVTIQVDGNLLATTDLSQYK 117

Query: 75  IGDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNR-------PISMKFNNVNTASIKN 134
              +W+ I  V+NL I G G L G G + W  NS  +       P S+  + VN   +  
Sbjct: 118 EHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSG 177

Query: 135 ISSFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCI 194
           ++  +SKFFH  ++  +++   +VTV AP +SPNTDGIH+  SSGI I ++ IG GDDCI
Sbjct: 178 VTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCI 237

Query: 195 SLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSS 254
           S+GPG+  +NI+ V CGPGHGISIGSLG+  +E+DV D+ V+D T   T  G RIK++  
Sbjct: 238 SIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYED 297

Query: 255 PYSSM-VSKVTFLNLQMNNVKNPIIIDQSYCPDS-CAPNKMNKSMVQIKDVRYQGITGSS 314
             S + VSK+ + N++M +  NPI ID  YCP+  C  N  +K  V +KDV ++ ITG+S
Sbjct: 298 AASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGASK--VTVKDVTFKNITGTS 357

Query: 315 NTPVAVDFECSQVLPCQGIVLQDINLTFNG-GGKTTSICHNVKGSASG 352
           +TP A+   C+  +PC G  + D+N+ ++G   KT +IC N KGS  G
Sbjct: 358 STPEAISLLCTAKVPCTGATMDDVNVEYSGTNNKTMAICTNAKGSTKG 402

[Close]

BLAST of CSPI03G04500 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.0e-76
Identity = 146/351 (41.60%), Postives = 218/351 (62.11%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHG-VIRIQLDGELLASSDKQFA 74
           QA+  AW  AC + G   VLIP +G + +  + +QGPC G  I  Q+DG + A +D    
Sbjct: 25  QAVMKAWKAACASQGPSTVLIP-KGNYNMGEVAMQGPCKGSKIGFQIDGVVKAPADPSKF 84

Query: 75  IGDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNR-------PISMKFNNVNTASIKN 134
             D W+S   ++ L + G G L G G +AW  N+ ++        ++++F+ +  A +++
Sbjct: 85  KSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAMNLRFDFLKHAMVRD 144

Query: 135 ISSFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCI 194
           I+S +SK FH  V    D+TF +VTV AP  S NTDGIH+  S G+ I ++ I TGDDCI
Sbjct: 145 ITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCI 204

Query: 195 SLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSS 254
           S+GPGS+ + I+ V+CGPGHGISIGSLG+  NE++V  +TV+  TF GT NG R+K+W +
Sbjct: 205 SIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPN 264

Query: 255 PYSSMVSKVTFLNLQMNNVKNPIIIDQSYCP-DSCAPNKMNKSMVQIKDVRYQGITGSSN 314
                 + +TF +L MNNV+NP+I+DQ YCP   C  ++   S +++ ++ +  I G+S 
Sbjct: 265 SPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQC--SRQAPSRIKLSNINFNNIRGTST 324

Query: 315 TPVAVDFECSQVLPCQGIVLQDINLTFNG-GGKTTSICHNVKGSASGQQLP 356
             VAV   CS  +PC  + + +INL++ G GG  TS C NVK + SG+Q+P
Sbjct: 325 GKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKPTFSGKQVP 372

[Close]

BLAST of CSPI03G04500 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.1e-73
Identity = 146/353 (41.36%), Postives = 214/353 (60.62%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAI 74
           QAL++AW  AC +     +L+P +G F V  + L+GPC   I +QL G L A +D     
Sbjct: 4   QALTTAWKEACASASPSTILVP-KGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIK 63

Query: 75  GDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSL--NRPI------SMKFNNVNTASIKN 134
           G  W+++  ++ L I G G   G G SAW  N    N PI      +++   V  + +++
Sbjct: 64  GLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILRD 123

Query: 135 ISSFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCI 194
           +++ DSK FH  V G +++TF+   + A   S NTDGIHI  S G+NI+++ I TGDDCI
Sbjct: 124 VTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDDCI 183

Query: 195 SLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSS 254
           SLG GSK INI+N+ CGPGHGIS+GSLG+  NEE V  + V++ T  G+ NG RIK+W  
Sbjct: 184 SLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPK 243

Query: 255 PYSSMVSKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNT 314
                 S++ F ++ MN+V  PI+IDQ YCP +    ++  S V++  + ++ I G+S T
Sbjct: 244 SEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEV-PSSVKLSKISFKNIKGTSTT 303

Query: 315 PVAVDFECSQVLPCQGIVLQDINLTFNG-GGKTTSICHNVKGSASGQQLPPSC 359
             AV   CS+  PC G+ L DI+LT++G GG  TS+C N+K +  G+Q+P  C
Sbjct: 304 KEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAIC 354

[Close]

BLAST of CSPI03G04500 vs. ExPASy TrEMBL
Match: A0A5D3BB58 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001870 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 7.8e-176
Identity = 306/350 (87.43%), Postives = 329/350 (94.00%), Query Frame = 0


Query: 16  ALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIG 75
           ALSSAWSGAC NNGGGVVLIPAQG+FLVLPLLLQGPCHGVIRIQLDGELLA SDKQFA G
Sbjct: 2   ALSSAWSGACENNGGGVVLIPAQGRFLVLPLLLQGPCHGVIRIQLDGELLAPSDKQFATG 61

Query: 76  DHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPI------SMKFNNVNTASIKNISS 135
           D+WLSI+NVNNLFIDGLGRLTGSG+SAWPS+S NRPI      SMK NN+NTASIKNISS
Sbjct: 62  DYWLSIQNVNNLFIDGLGRLTGSGSSAWPSHSANRPIVRFHLSSMKLNNINTASIKNISS 121

Query: 136 FDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLG 195
           +DSK FHFAVHGSRDVTFD+VTVVAP +SPNTDGIHIS+SSGINIMHSTIGTGDDCISLG
Sbjct: 122 YDSKLFHFAVHGSRDVTFDHVTVVAPGDSPNTDGIHISSSSGINIMHSTIGTGDDCISLG 181

Query: 196 PGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYS 255
           PGSK INI+NV+CGPGHGISIGSLGK PNEE+VF+VTVRDSTF+GT+NGARIKSWSS YS
Sbjct: 182 PGSKSINITNVYCGPGHGISIGSLGKYPNEENVFEVTVRDSTFVGTTNGARIKSWSSSYS 241

Query: 256 SMVSKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVA 315
           SMVSKVTF+NLQMNNVKNPIIIDQSYCPDSC PN+M+KS VQI+DVRY+GI GSSNT VA
Sbjct: 242 SMVSKVTFVNLQMNNVKNPIIIDQSYCPDSCGPNEMSKSKVQIEDVRYEGIRGSSNTQVA 301

Query: 316 VDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 360
           VDFECSQVLPCQGIVLQDINLTFNGGG++TSICHNVKGSA GQQLPPSCL
Sbjct: 302 VDFECSQVLPCQGIVLQDINLTFNGGGQSTSICHNVKGSAFGQQLPPSCL 351

[Close]

BLAST of CSPI03G04500 vs. ExPASy TrEMBL
Match: A0A0A0L7H9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 6.4e-162
Identity = 280/346 (80.92%), Postives = 309/346 (89.31%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAI 74
           +ALSSAWS AC NNGGG+VLIPA+G+FLVLPLLLQGPCHG IRIQLDGELLA  D+ FA 
Sbjct: 15  KALSSAWSEACTNNGGGIVLIPAKGRFLVLPLLLQGPCHGFIRIQLDGELLAPLDEHFAT 74

Query: 75  GDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDSKF 134
           GD+WLSI+ VNNLFIDGLG L G G++AWPS S NRP+SM+ N +N A I NI S++SK 
Sbjct: 75  GDYWLSIDQVNNLFIDGLGSLDGRGSTAWPSTSHNRPVSMRLNGINNARITNIKSYNSKL 134

Query: 135 FHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGSKM 194
           FHFA+HG RDVTFD+VTV+APANSPNTDGIHIS+SSGINIMHSTIGTGDDCISLGPGSK 
Sbjct: 135 FHFAMHGCRDVTFDHVTVIAPANSPNTDGIHISSSSGINIMHSTIGTGDDCISLGPGSKF 194

Query: 195 INISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMVSK 254
           INI+N+ CGPGHGISIGSLGK PNEEDVF+VTVRDSTFIGTSNG RIK+WSS YSSMVSK
Sbjct: 195 INITNIQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFIGTSNGVRIKTWSSSYSSMVSK 254

Query: 255 VTFLNLQMNNVKNPIIIDQSYCPDSCAPNK-MNKSMVQIKDVRYQGITGSSNTPVAVDFE 314
           VTFLNLQMNNVKNPIIIDQ+YCP SC   K M+KSMVQIKDVRY+GI GSSNT VAVDFE
Sbjct: 255 VTFLNLQMNNVKNPIIIDQNYCPGSCGSQKMMSKSMVQIKDVRYEGIRGSSNTQVAVDFE 314

Query: 315 CSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 360
           CS+V+PCQGIVLQDINL FNGGG+TTS CHNV+GSA G QLPPSCL
Sbjct: 315 CSEVVPCQGIVLQDINLPFNGGGRTTSNCHNVRGSAFGHQLPPSCL 360

[Close]

BLAST of CSPI03G04500 vs. ExPASy TrEMBL
Match: A0A1S4DX91 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103491147 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 3.0e-159
Identity = 277/345 (80.29%), Postives = 305/345 (88.41%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAI 74
           +ALS+AWS AC NNGGGVVLIPA G+FLVLPLLLQGPCHG I IQLDGELLA  ++    
Sbjct: 15  KALSTAWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQLDGELLAPLNEN--T 74

Query: 75  GDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDSKF 134
           GD+WLSI+ VNNLFIDGLGRL G G++AWPS S NRPISMK   +N A I NI S++SK 
Sbjct: 75  GDYWLSIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGINNARISNIKSYNSKL 134

Query: 135 FHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGSKM 194
           FHFAVHG RDVTFD+VTV+APANSPNTDGIH+S SSGINIMHSTIGTGDDCISLGPGSK 
Sbjct: 135 FHFAVHGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIGTGDDCISLGPGSKF 194

Query: 195 INISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMVSK 254
           INI+NV CGPGHGISIGSLGK PNEEDVF+VTVRDSTF+GT+NGARIK+WSS YS+MVSK
Sbjct: 195 INITNVQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGARIKTWSSSYSNMVSK 254

Query: 255 VTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVAVDFEC 314
           VTFLNLQMNNVKNPIIIDQSYCP SC PN+M++S VQIKDVRY+GI GSSNT VAVDFEC
Sbjct: 255 VTFLNLQMNNVKNPIIIDQSYCPHSCGPNEMSQSKVQIKDVRYEGIRGSSNTQVAVDFEC 314

Query: 315 SQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 360
           SQV PCQGIVLQDINL FNGGG+TT+ CHNV+GSA G QLPPSCL
Sbjct: 315 SQVFPCQGIVLQDINLPFNGGGRTTANCHNVRGSAFGHQLPPSCL 357

[Close]

BLAST of CSPI03G04500 vs. ExPASy TrEMBL
Match: A0A5D3B9J1 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001860 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.9e-159
Identity = 276/345 (80.00%), Postives = 305/345 (88.41%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAI 74
           +ALS+AWS AC NNGGGVVLIPA G+FLVLPLLLQGPCHG I IQLDGELLA  ++    
Sbjct: 15  KALSTAWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQLDGELLAPLNEN--T 74

Query: 75  GDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDSKF 134
           GD+WLSI+ VNNLFIDGLGRL G G++AWPS S NRPISMK   +N A I NI S++SK 
Sbjct: 75  GDYWLSIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGINNARISNIKSYNSKL 134

Query: 135 FHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGSKM 194
           FHFAVHG RDVTFD+VTV+APANSPNTDGIH+S SSGINIMHSTIGTGDDCISLGPGSK 
Sbjct: 135 FHFAVHGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIGTGDDCISLGPGSKF 194

Query: 195 INISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMVSK 254
           INI+NV CGPGHGISIGSLGK PNEEDVF+VTVRDSTF+GT+NGARIK+WSS YS+MVSK
Sbjct: 195 INITNVQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGARIKTWSSSYSNMVSK 254

Query: 255 VTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVAVDFEC 314
           +TFLNLQMNNVKNPIIIDQSYCP SC PN+M++S VQIKDVRY+GI GSSNT VAVDFEC
Sbjct: 255 ITFLNLQMNNVKNPIIIDQSYCPHSCGPNEMSQSKVQIKDVRYEGIRGSSNTQVAVDFEC 314

Query: 315 SQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 360
           SQV PCQGIVLQDINL FNGGG+TT+ CHNV+GSA G QLPPSCL
Sbjct: 315 SQVFPCQGIVLQDINLPFNGGGRTTANCHNVRGSAFGHQLPPSCL 357

[Close]

BLAST of CSPI03G04500 vs. ExPASy TrEMBL
Match: A0A0A0L660 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063620 PE=3 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 1.1e-137
Identity = 245/246 (99.59%), Postives = 245/246 (99.59%), Query Frame = 0


Query: 114 MKFNNVNTASIKNISSFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGIN 173
           MKFNNVNTASIKNISSFDSKFFHFAVH SRDVTFDNVTVVAPANSPNTDGIHISTSSGIN
Sbjct: 1   MKFNNVNTASIKNISSFDSKFFHFAVHASRDVTFDNVTVVAPANSPNTDGIHISTSSGIN 60

Query: 174 IMHSTIGTGDDCISLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFI 233
           IMHSTIGTGDDCISLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFI
Sbjct: 61  IMHSTIGTGDDCISLGPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFI 120

Query: 234 GTSNGARIKSWSSPYSSMVSKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIK 293
           GTSNGARIKSWSSPYSSMVSKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIK
Sbjct: 121 GTSNGARIKSWSSPYSSMVSKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIK 180

Query: 294 DVRYQGITGSSNTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 353
           DVRYQGITGSSNTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ
Sbjct: 181 DVRYQGITGSSNTPVAVDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQ 240

Query: 354 LPPSCL 360
           LPPSCL
Sbjct: 241 LPPSCL 246

[Close]

BLAST of CSPI03G04500 vs. NCBI nr
Match: XP_004149596.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 702.6 bits (1812), Expect = 1.8e-198
Identity = 345/347 (99.42%), Postives = 345/347 (99.42%), Query Frame = 0


Query: 13  YLQALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQF 72
           YL ALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQF
Sbjct: 3   YLVALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQF 62

Query: 73  AIGDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDS 132
           AIGDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDS
Sbjct: 63  AIGDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDS 122

Query: 133 KFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGS 192
           KFFHFAVH SRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGS
Sbjct: 123 KFFHFAVHASRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGS 182

Query: 193 KMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMV 252
           KMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMV
Sbjct: 183 KMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMV 242

Query: 253 SKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVAVDF 312
           SKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVAVDF
Sbjct: 243 SKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVAVDF 302

Query: 313 ECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 360
           ECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL
Sbjct: 303 ECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 349

[Close]

BLAST of CSPI03G04500 vs. NCBI nr
Match: KAA0065794.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYJ96533.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 626.3 bits (1614), Expect = 1.6e-175
Identity = 306/350 (87.43%), Postives = 329/350 (94.00%), Query Frame = 0


Query: 16  ALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIG 75
           ALSSAWSGAC NNGGGVVLIPAQG+FLVLPLLLQGPCHGVIRIQLDGELLA SDKQFA G
Sbjct: 2   ALSSAWSGACENNGGGVVLIPAQGRFLVLPLLLQGPCHGVIRIQLDGELLAPSDKQFATG 61

Query: 76  DHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPI------SMKFNNVNTASIKNISS 135
           D+WLSI+NVNNLFIDGLGRLTGSG+SAWPS+S NRPI      SMK NN+NTASIKNISS
Sbjct: 62  DYWLSIQNVNNLFIDGLGRLTGSGSSAWPSHSANRPIVRFHLSSMKLNNINTASIKNISS 121

Query: 136 FDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLG 195
           +DSK FHFAVHGSRDVTFD+VTVVAP +SPNTDGIHIS+SSGINIMHSTIGTGDDCISLG
Sbjct: 122 YDSKLFHFAVHGSRDVTFDHVTVVAPGDSPNTDGIHISSSSGINIMHSTIGTGDDCISLG 181

Query: 196 PGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYS 255
           PGSK INI+NV+CGPGHGISIGSLGK PNEE+VF+VTVRDSTF+GT+NGARIKSWSS YS
Sbjct: 182 PGSKSINITNVYCGPGHGISIGSLGKYPNEENVFEVTVRDSTFVGTTNGARIKSWSSSYS 241

Query: 256 SMVSKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVA 315
           SMVSKVTF+NLQMNNVKNPIIIDQSYCPDSC PN+M+KS VQI+DVRY+GI GSSNT VA
Sbjct: 242 SMVSKVTFVNLQMNNVKNPIIIDQSYCPDSCGPNEMSKSKVQIEDVRYEGIRGSSNTQVA 301

Query: 316 VDFECSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 360
           VDFECSQVLPCQGIVLQDINLTFNGGG++TSICHNVKGSA GQQLPPSCL
Sbjct: 302 VDFECSQVLPCQGIVLQDINLTFNGGGQSTSICHNVKGSAFGQQLPPSCL 351

[Close]

BLAST of CSPI03G04500 vs. NCBI nr
Match: XP_031738767.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 580.1 bits (1494), Expect = 1.3e-161
Identity = 280/346 (80.92%), Postives = 309/346 (89.31%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAI 74
           +ALSSAWS AC NNGGG+VLIPA+G+FLVLPLLLQGPCHG IRIQLDGELLA  D+ FA 
Sbjct: 15  KALSSAWSEACTNNGGGIVLIPAKGRFLVLPLLLQGPCHGFIRIQLDGELLAPLDEHFAT 74

Query: 75  GDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDSKF 134
           GD+WLSI+ VNNLFIDGLG L G G++AWPS S NRP+SM+ N +N A I NI S++SK 
Sbjct: 75  GDYWLSIDQVNNLFIDGLGSLDGRGSTAWPSTSHNRPVSMRLNGINNARITNIKSYNSKL 134

Query: 135 FHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGSKM 194
           FHFA+HG RDVTFD+VTV+APANSPNTDGIHIS+SSGINIMHSTIGTGDDCISLGPGSK 
Sbjct: 135 FHFAMHGCRDVTFDHVTVIAPANSPNTDGIHISSSSGINIMHSTIGTGDDCISLGPGSKF 194

Query: 195 INISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMVSK 254
           INI+N+ CGPGHGISIGSLGK PNEEDVF+VTVRDSTFIGTSNG RIK+WSS YSSMVSK
Sbjct: 195 INITNIQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFIGTSNGVRIKTWSSSYSSMVSK 254

Query: 255 VTFLNLQMNNVKNPIIIDQSYCPDSCAPNK-MNKSMVQIKDVRYQGITGSSNTPVAVDFE 314
           VTFLNLQMNNVKNPIIIDQ+YCP SC   K M+KSMVQIKDVRY+GI GSSNT VAVDFE
Sbjct: 255 VTFLNLQMNNVKNPIIIDQNYCPGSCGSQKMMSKSMVQIKDVRYEGIRGSSNTQVAVDFE 314

Query: 315 CSQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 360
           CS+V+PCQGIVLQDINL FNGGG+TTS CHNV+GSA G QLPPSCL
Sbjct: 315 CSEVVPCQGIVLQDINLPFNGGGRTTSNCHNVRGSAFGHQLPPSCL 360

[Close]

BLAST of CSPI03G04500 vs. NCBI nr
Match: XP_016900599.1 (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 571.2 bits (1471), Expect = 6.1e-159
Identity = 277/345 (80.29%), Postives = 305/345 (88.41%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAI 74
           +ALS+AWS AC NNGGGVVLIPA G+FLVLPLLLQGPCHG I IQLDGELLA  ++    
Sbjct: 15  KALSTAWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQLDGELLAPLNEN--T 74

Query: 75  GDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDSKF 134
           GD+WLSI+ VNNLFIDGLGRL G G++AWPS S NRPISMK   +N A I NI S++SK 
Sbjct: 75  GDYWLSIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGINNARISNIKSYNSKL 134

Query: 135 FHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGSKM 194
           FHFAVHG RDVTFD+VTV+APANSPNTDGIH+S SSGINIMHSTIGTGDDCISLGPGSK 
Sbjct: 135 FHFAVHGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIGTGDDCISLGPGSKF 194

Query: 195 INISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMVSK 254
           INI+NV CGPGHGISIGSLGK PNEEDVF+VTVRDSTF+GT+NGARIK+WSS YS+MVSK
Sbjct: 195 INITNVQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGARIKTWSSSYSNMVSK 254

Query: 255 VTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVAVDFEC 314
           VTFLNLQMNNVKNPIIIDQSYCP SC PN+M++S VQIKDVRY+GI GSSNT VAVDFEC
Sbjct: 255 VTFLNLQMNNVKNPIIIDQSYCPHSCGPNEMSQSKVQIKDVRYEGIRGSSNTQVAVDFEC 314

Query: 315 SQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 360
           SQV PCQGIVLQDINL FNGGG+TT+ CHNV+GSA G QLPPSCL
Sbjct: 315 SQVFPCQGIVLQDINLPFNGGGRTTANCHNVRGSAFGHQLPPSCL 357

[Close]

BLAST of CSPI03G04500 vs. NCBI nr
Match: KAA0065793.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYJ96532.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 570.9 bits (1470), Expect = 8.0e-159
Identity = 276/345 (80.00%), Postives = 305/345 (88.41%), Query Frame = 0


Query: 15  QALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAI 74
           +ALS+AWS AC NNGGGVVLIPA G+FLVLPLLLQGPCHG I IQLDGELLA  ++    
Sbjct: 15  KALSTAWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQLDGELLAPLNEN--T 74

Query: 75  GDHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDSKF 134
           GD+WLSI+ VNNLFIDGLGRL G G++AWPS S NRPISMK   +N A I NI S++SK 
Sbjct: 75  GDYWLSIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGINNARISNIKSYNSKL 134

Query: 135 FHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGSKM 194
           FHFAVHG RDVTFD+VTV+APANSPNTDGIH+S SSGINIMHSTIGTGDDCISLGPGSK 
Sbjct: 135 FHFAVHGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIGTGDDCISLGPGSKF 194

Query: 195 INISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMVSK 254
           INI+NV CGPGHGISIGSLGK PNEEDVF+VTVRDSTF+GT+NGARIK+WSS YS+MVSK
Sbjct: 195 INITNVQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGARIKTWSSSYSNMVSK 254

Query: 255 VTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTPVAVDFEC 314
           +TFLNLQMNNVKNPIIIDQSYCP SC PN+M++S VQIKDVRY+GI GSSNT VAVDFEC
Sbjct: 255 ITFLNLQMNNVKNPIIIDQSYCPHSCGPNEMSQSKVQIKDVRYEGIRGSSNTQVAVDFEC 314

Query: 315 SQVLPCQGIVLQDINLTFNGGGKTTSICHNVKGSASGQQLPPSCL 360
           SQV PCQGIVLQDINL FNGGG+TT+ CHNV+GSA G QLPPSCL
Sbjct: 315 SQVFPCQGIVLQDINLPFNGGGRTTANCHNVRGSAFGHQLPPSCL 357

[Close]

BLAST of CSPI03G04500 vs. TAIR 10
Match: AT2G15450.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.3e-76
Identity = 152/355 (42.82%), Postives = 210/355 (59.15%), Query Frame = 0


Query: 16  ALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIG 75
           A +S WS AC    G   +   +G F +  +   GPC   I   +DG LLA ++      
Sbjct: 49  AFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKNPIEFIIDGTLLAPANPSDIKQ 108

Query: 76  DHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNR-------PISMKFNNVNTASIKNIS 135
           D W++   +NNL I G G L G G  +WP N  +         ++M F  VN ++IK+I+
Sbjct: 109 DTWINFRYINNLSISGSGTLDGQGKQSWPHNDCHTNPNCPKLAMTMGFAFVNNSNIKDIT 168

Query: 136 SFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISL 195
           S +SK  HF            VT+ AP +SPNTDGI + + S I I  + IGTGDDCI++
Sbjct: 169 SLNSKMGHFNFFSVHHFNITGVTITAPGDSPNTDGIKMGSCSNIQISDTNIGTGDDCIAI 228

Query: 196 GPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPY 255
             G+  +NISNV+CGPGHGIS+GSLGKN +E+DV D+ VRD  F GTS+G RIK+W S  
Sbjct: 229 LSGTTNLNISNVNCGPGHGISVGSLGKNKDEKDVKDLIVRDVIFNGTSDGIRIKNWESSA 288

Query: 256 SS-MVSKVTFLNLQMNNVKNPIIIDQSYCP-DSCAPNKMNKSMVQIKDVRYQGITGSSNT 315
           S  +VS   + N+QM +V  PI IDQ YCP   C   +  +S VQI++++ + I G+S  
Sbjct: 289 SKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCEHERKGESHVQIQNLKLKNIYGTSKN 348

Query: 316 PVAVDFECSQVLPCQGIVLQDINLTFNG--GGKTTSICHNVKGSASGQQLPPSCL 360
            VAV+ +CS++ PC+ + L DIN+  NG   G +TS+C NV G A G+  PP CL
Sbjct: 349 KVAVNLQCSKIFPCKNVELIDINIKQNGVKDGSSTSVCENVDGFARGKMFPPHCL 403

[Close]

BLAST of CSPI03G04500 vs. TAIR 10
Match: AT4G13760.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 276.6 bits (706), Expect = 2.9e-74
Identity = 148/353 (41.93%), Postives = 212/353 (60.06%), Query Frame = 0


Query: 18  SSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIGDH 77
           +S W+ AC    G   +   +G F +  +   GPC   I   +DG LLA ++      D 
Sbjct: 51  TSVWNRACRRKSGSSKIYVPKGTFYLGGVEFVGPCKNPIEFLIDGTLLAPANPNNIKQDT 110

Query: 78  WLSIENVNNLFIDGLGRLTGSGASAWPSNSLNR-------PISMKFNNVNTASIKNISSF 137
           W+  + +N+L I G G L G G  +WP N  ++        ++M F  VN ++IK+I+S 
Sbjct: 111 WIKFKYINDLSISGSGTLDGQGKQSWPLNDCHKNPNCPKLAMTMGFAFVNNSNIKDITSL 170

Query: 138 DSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGP 197
           +SK  HF            VT+ AP NSPNTDGI + + S I+I ++ IGTGDDCI++  
Sbjct: 171 NSKMGHFNFFSVHHFNITGVTITAPGNSPNTDGIKMGSCSNIHISNTNIGTGDDCIAILS 230

Query: 198 GSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSS 257
           G+  ++ISNV+CGPGHGIS+GSLGKN +E+DV D+T+RD  F GTS+G RIK+W S  S 
Sbjct: 231 GTTNLDISNVNCGPGHGISVGSLGKNKDEKDVKDLTIRDVIFNGTSDGIRIKTWESSASK 290

Query: 258 -MVSKVTFLNLQMNNVKNPIIIDQSYCP-DSCAPNKMNKSMVQIKDVRYQGITGSSNTPV 317
            +VS   + N+QM +V  PI IDQ YCP   C   +  +S VQI++++ + I G+S   V
Sbjct: 291 ILVSNFLYENIQMIDVGKPINIDQKYCPHPPCEHEQKGESHVQIQNLKLKNIYGTSKNKV 350

Query: 318 AVDFECSQVLPCQGIVLQDINLTFNG--GGKTTSICHNVKGSASGQQLPPSCL 360
           AV+ +CS+  PC+ I L DIN+T NG     +T +C NV GS SG+ +P  C+
Sbjct: 351 AVNLQCSKRFPCKNIELIDINITNNGLVDSFSTLVCENVDGSVSGKMVPQHCI 403

[Close]

BLAST of CSPI03G04500 vs. TAIR 10
Match: AT2G26620.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.8e-74
Identity = 151/354 (42.66%), Postives = 210/354 (59.32%), Query Frame = 0


Query: 16  ALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIG 75
           A +S WS AC    G   +   +G F +  +   GPC   I   +DG LLA ++      
Sbjct: 49  AFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKNPIEFIIDGTLLAPANPNDIKQ 108

Query: 76  DHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNR-------PISMKFNNVNTASIKNIS 135
           D W++   +NNL I G G L G G  +WP N  ++        ISM F  VN ++IK+I+
Sbjct: 109 DTWINFRYINNLSISGSGTLDGQGKQSWPLNDCHKNPNCPKLAISMGFAFVNNSNIKDIT 168

Query: 136 SFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISL 195
           S +SK  HF            VT+ AP++SPNTDGI + + S I I ++ IGTGDDCI++
Sbjct: 169 SLNSKMGHFNFFFVHHFNITGVTITAPSDSPNTDGIKMGSCSNIQISNTNIGTGDDCIAI 228

Query: 196 GPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPY 255
             G+  +NISN++CGPGHGIS+GSLGKN +E+DV D+ VRD  F GTS+G RIK+W S  
Sbjct: 229 LSGTTKLNISNINCGPGHGISVGSLGKNKDEKDVKDLFVRDVIFNGTSDGIRIKTWESSA 288

Query: 256 SS-MVSKVTFLNLQMNNVKNPIIIDQSYCPDSCAPNKMNKSMVQIKDVRYQGITGSSNTP 315
           S  +VS   + N+QM +V  PI IDQ YCP     ++  KS VQI+D++ + I G+S   
Sbjct: 289 SKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCEHE-RKSHVQIQDLKLKNIYGTSKNK 348

Query: 316 VAVDFECSQVLPCQGIVLQDINLTFNG--GGKTTSICHNVKGSASGQQLPPSCL 360
           VAV+ +CS+  PC+ + L DIN+  NG   G + ++C NV G A G+  P  CL
Sbjct: 349 VAVNLQCSKSFPCKNVELIDINIKQNGLEDGSSITVCENVDGFARGKMFPQHCL 401

[Close]

BLAST of CSPI03G04500 vs. TAIR 10
Match: AT1G43080.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 8.5e-74
Identity = 149/355 (41.97%), Postives = 210/355 (59.15%), Query Frame = 0


Query: 16  ALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIG 75
           A +S W  AC    G   +   +G F +  +   GPC   I   +DG LLA ++ +    
Sbjct: 49  AFTSVWKRACTRISGSSKIYVPKGTFYLGGVEFVGPCKNPIEFVIDGTLLAPANPRDIKQ 108

Query: 76  DHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNR-------PISMKFNNVNTASIKNIS 135
           D W++   +NNL I G G L G G  +WP N  ++        ++M F  VN + IK+I+
Sbjct: 109 DTWINFRYINNLSISGSGTLDGQGKYSWPLNDCHKNTNCPKLAMTMGFAFVNNSRIKDIT 168

Query: 136 SFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISL 195
           S +SK  HF            VT+ AP +SPNTDGI + + S I+I ++ IGTGDDCI++
Sbjct: 169 SLNSKMGHFNFFSVHRFNITGVTITAPGDSPNTDGIKMGSCSNIHISNTNIGTGDDCIAI 228

Query: 196 GPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPY 255
             G+  ++ISN+ CGPGHGIS+GSLGKN +E+DV  +TVRD+ F GTS+G RIK+W S  
Sbjct: 229 LSGTTNLDISNIKCGPGHGISVGSLGKNKDEKDVKHLTVRDTVFNGTSDGIRIKTWESSA 288

Query: 256 SS-MVSKVTFLNLQMNNVKNPIIIDQSYCP-DSCAPNKMNKSMVQIKDVRYQGITGSSNT 315
           S  +VS   + N+QM +V  PI IDQ YCP   C   K  +S VQI+D++ + I G+SN 
Sbjct: 289 SKIVVSNFIYENIQMIDVGKPINIDQKYCPHPPCEHEKKGESHVQIQDIKLKNIYGTSNN 348

Query: 316 PVAVDFECSQVLPCQGIVLQDINLTFNG--GGKTTSICHNVKGSASGQQLPPSCL 360
            VAV+ +CS+  PC+ + L DINL   G   G +T++C NV GS   + +P  CL
Sbjct: 349 IVAVNLQCSKSFPCKNVELIDINLKHTGLEKGHSTAMCENVDGSVRSKMVPQHCL 403

[Close]

BLAST of CSPI03G04500 vs. TAIR 10
Match: AT2G15460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 3.2e-73
Identity = 148/351 (42.17%), Postives = 207/351 (58.97%), Query Frame = 0


Query: 16  ALSSAWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIG 75
           A +S WS AC    G   +   +G F +  +   GPC   I   +DG LLA ++      
Sbjct: 49  AFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKNPIEFIIDGTLLAPANPSDIKQ 108

Query: 76  DHWLSIENVNNLFIDGLGRLTGSGASAWPSNSLNR-------PISMKFNNVNTASIKNIS 135
           D W++   +NNL I G G L G G  +WP N  +         ++M F  VN ++IK+I+
Sbjct: 109 DTWINFRYINNLSISGSGTLDGQGKQSWPHNDCHTNPNCPKLAMTMGFAFVNNSNIKDIT 168

Query: 136 SFDSKFFHFAVHGSRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISL 195
           S +SK  HF            VT+ AP +SPNTDGI + + S I I  + IGTGDDCI++
Sbjct: 169 SLNSKMGHFNFFSVHHFNITGVTITAPGDSPNTDGIKMGSCSNIQISDTNIGTGDDCIAI 228

Query: 196 GPGSKMINISNVHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPY 255
             G+  +NISNV+CGPGHGIS+GSLGK+ +E+DV D+ VRD  F GTS+G RIK+W S  
Sbjct: 229 LSGTTNLNISNVNCGPGHGISVGSLGKSKDEKDVKDLIVRDVIFNGTSDGIRIKTWESSA 288

Query: 256 SS-MVSKVTFLNLQMNNVKNPIIIDQSYCP-DSCAPNKMNKSMVQIKDVRYQGITGSSNT 315
           S  +VS   + N+QM +V  PI IDQ YCP   C   +  +S VQI++++ + I G+S  
Sbjct: 289 SKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCEHERKGESHVQIQNLKLKNIYGTSKN 348

Query: 316 PVAVDFECSQVLPCQGIVLQDINLTFNG--GGKTTSICHNVKGSASGQQLP 356
            VAV+ +CS++ PC+ + L DIN+  NG   G +TS+C NV G A G+  P
Sbjct: 349 KVAVNLQCSKIFPCKNVELIDINIKQNGVKDGSSTSVCENVDGFARGKMFP 399

The following BLAST results are available for this feature:

BLAST of CSPI03G04500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P35339	2.2e-79	43.97	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]
P26216	1.1e-78	44.25	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1	[more]
P35338	1.6e-77	43.68	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1	[more]
Q6H9K0	3.0e-76	41.60	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
P24548	3.1e-73	41.36	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]

BLAST of CSPI03G04500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BB58	7.8e-176	87.43	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A0A0L7H9	6.4e-162	80.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1	[more]
A0A1S4DX91	3.0e-159	80.29	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103491147 PE=3 SV=1	[more]
A0A5D3B9J1	3.9e-159	80.00	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A0A0L660	1.1e-137	99.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063620 PE=3 SV=1	[more]

BLAST of CSPI03G04500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149596.1	1.8e-198	99.42	exopolygalacturonase [Cucumis sativus]	[more]
KAA0065794.1	1.6e-175	87.43	exopolygalacturonase-like [Cucumis melo var. makuwa] >TYJ96533.1 exopolygalactur...	[more]
XP_031738767.1	1.3e-161	80.92	exopolygalacturonase [Cucumis sativus]	[more]
XP_016900599.1	6.1e-159	80.29	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
KAA0065793.1	8.0e-159	80.00	exopolygalacturonase-like [Cucumis melo var. makuwa] >TYJ96532.1 exopolygalactur...	[more]

BLAST of CSPI03G04500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15450.1	5.3e-76	42.82	Pectin lyase-like superfamily protein	[more]
AT4G13760.1	2.9e-74	41.93	Pectin lyase-like superfamily protein	[more]
AT2G26620.1	3.8e-74	42.66	Pectin lyase-like superfamily protein	[more]
AT1G43080.1	8.5e-74	41.97	Pectin lyase-like superfamily protein	[more]
AT2G15460.1	3.2e-73	42.17	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 169..190 e-value: 4700.0 score: 2.4 coord: 252..273 e-value: 8500.0 score: 0.6 coord: 142..168 e-value: 8.3 score: 15.4 coord: 222..243 e-value: 15.0 score: 14.5 coord: 192..212 e-value: 8000.0 score: 0.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 14..356 e-value: 2.7E-98 score: 331.7
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 25..349 e-value: 1.1E-88 score: 297.4
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 199..212
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 15..358
None	No IPR available	PANTHER	PTHR31375:SF246	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 15..358
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 15..359

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G04500.1	CSPI03G04500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity