CSPI03G04360 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G04360
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	THO complex subunit 3
Location	Chr3: 3558117 .. 3561373 (+)
RNA-Seq Expression	CSPI03G04360
Synteny	CSPI03G04360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAGGATTTGATGTATTGAACAATTTTTTCACTCAAAAATCCATTTTAGTATTCGCATGGAAAGTCCCTCTAATCACAGGATTTGAAATCGTTCCATAGCAGCAGGACATGGAGGAATCAGCCCAAGCTTTCAAGAATCTTCACAGCAGAGAGTATCAAGGTCACAAGAAGAAGGTTTTGTTTTTTCCTCTTATCATATTCCTTCTTTCATGCTGTTTTCTAAGCCGATTTTATTCACCCTTTTAATATTTTTGATTCGTCTTTTCTTCAGGTACATTCTGTGGCATGGAATTGCACGGGCATGAAGCTTGCTTCCGGTTCTGTTGATCAAACTGCTCGAGTTTGGCATATTGAGCCTCACGGACATGTATGCTTTCTCTTCTTTGATTCTCCCGTGAAATCCTCCTCAAATTTCTCGATTAGGGTTTTGTTTATTTTTTGTTTTTCTTTTTTGGGGTGCTGTAAATGGTCGAATTTGAGCTTAAATCTTCTTTTAATACTTGTCTCTTCATCCCACCGATTTGATAAATGTATATGTGGGGATTTACTATAGTTGAATAGCATACATAAGTGAAGTTATGAAACAAAAGGGTTAGGGAAAGCTTCAACTTTTGAGTTTCCTACTTCCACTTTTTACGTATTGTTCCATAAGCTCTTGCTCTTGTGTTTTCCTTTCTTTTATGTTCTGGAAAATGGGAGTACGATTTACAGAGTTCTTTAGTTGTGTATATATGTGTTAGTGTGTTACGCTTGATTCCTCCTGAGACCTGTTTTGAATGGTTGCTATGGTTTTACCACAGTATTTAGTGTTCGCATTGTTTGTTTGTCTTTACTTACAACCCTGAACAAATCATTTCAATCATGTCAGACGATGTTTGCTTCAGGGTAAGGTTAAGGATGTTGAGTTGAAAGGGCACACTGATAGTGTAGATCAGCTTTGCTGGGACCCTAAACATTCTGATCTTATAGCGACTGCATCTGGAGACAAGACTGTTCGACTATGGGATGCTCGCAGTAAGTGTTTGAAACTGAGATTTTAAAAGTAACCGATCCAACTGTATTTTAAGATATGGTTCATTTTTTGTTTTGTTCTATAGATGGGAAATGCTCACAGCAAGCTGAGCTCAGTGGGGAAAATATCAACATCACCTACAAACCTGATGGGACACACATCGCTGTTGGAAATAGGGTATGTTCTAGGGTGAATTTTGTGCATTGTAAAGCCAATTTTTATTTGTTATTGGTTGAATGTACATCTCAAGAAGTTTATAAATTATATCCCTTGGAGATGTTGACCATTTCAGTACTCACTGTTTTCTCACATCATTTTGCAGGATGATGAACTTACAATTCTGGATGTTAGGAAGTTTAAACCAGTTCACAAGCGCAAGTTCAACTATGAGGTAATGTTTATCTAAGATAAACTACGGTAACCTTGACCATTAGCCATTTATGGAAGCATAAGAGAGGATGATTGTGTTAAATGTTGAGAAATTTGATACAGGACATTAAAGATGACTGCTTTGACATGTTGATGTTGGTCTTACTTTAAAAATTAGAAGTCCAATTTTAGTAGAGGCCGTCTTTACTCTGTTCTTGAAAGCTCTTGACAGGCCCCTCATTATTAATGTATATAGGAGGAAACATGTGAAGGAGATGGGCTAAATGTATCTTGTAAATAGGGTGATGTAATAGTAATATGGGCCCTTTAGCGGGTTGATTTTGTAACTGGCGCGGGCATTAATTTATAGTATTAAGTCTTTTGTGAACAGAAAGGGCATGATTAGACTGTGATAATCTTTTGTTTTACTTTGATTATCTACATTGACAGGAGTTTTTCATTATCTTTTTCCAGGTGAATGAAATTGCATGGAACATGACTGGGGAGATGTTTTTCCTGACAACTGGAAATGGTAACGATATGCATTAGTGACATTTCTTTAAATGCAATAACTGAGGACATATTGTTGAACTCTGTCAAATAAAATTTCATGTCAATTTCTTGTGCTCAGGTACTGTTGAAGTACTAGCATACCCATCGCTTCGACCAATCGAAACTCTTATGGCTCATACAGCGGGTTGTTACTGCATTGCGATTGACCCTGTTGGAGGGTGAGTTCGAAAGTTGATTTAAATTGAGTGAAGCTTCTTATCTATGTACAACTCTTCCATGGAATATGCACTCATGATTTCTATTATTTAAAAACTCGTGGTTTCAAACTTTCAATTGCTTGCTTCATTTATTGTGAAAACTTCTTCTGCAATTAGCGATAGGGAAAGATAAATGACTTGTTACAATCAATAGGTATTTTGCAGTTGGAAGTGCTGATTCATTAGTTAGCCTATGGGATATATCGCAGATGCTTTGTGTGCGAACATTTACAAAACTTGAGTAAGTTTTTGTTTGATTTATACGACAATTTTGTTTTCTTCTTTCCTCTTTTCTAGATTTGCAACACTGCATTACGCAGCGACTTGACTTGTTTATATACTGAAGGATAATACTTTCTTGTCACTTATTTAACTTCCTTAGATGGCCTGTTAGAACAATAAGTTTCAACCACACAGGAGAATACATTGCTTCTGCCAGTGAGGATTTGTTCATTGATATAGTAAGCATTCATTCTAGTCTCTCTCCTTTACTTTAATATCTGATCATGTTACAGTCTCCCCTCTGTTTTTGCTGAAGAGGATTCGTGCCAAAACTGGTTAATGGATGGTGTTTTTTCTTTTGCTTACAGTCAAATGTTCAAACGGGACGAACGGTTCATCAGATTCCTTGTCGGGCTGCAATGAACAGTGTGGAGTGGAATCCTAAACATAACTTACTTGCATATGCTGGGGATGACAAGAACAAGTATCAGGCTGATGAAGGCAAGTTATCTGTGCATTGGTGTTTCCTCCTTATCTTAATATTTGAAGTCACTTCACAAGAAGTGTTATCATTTTCTTGTTGCAGGTATTTTTAGGATCTTTGGGTTTGAAAGTCCATGAGAAATGGATCAACCATGGAGAAATTTCCAATTCTGTGATACTTTATTATGTTCCTTCATTTCTGGCAAAATGTATTGAATTTCAGTTAGTGGCTGTTACCATAATAACTTAGTCAAATTTTCCCTTGAAACAACAATCTGTGGTTGTATTACCTCTTGAAAAGTTCACCATGATAAAATCTTAACTTGAAACAACCATTCCTACACACCTGATGCTTAGAAGTTCCATCTTTTTAACTCGAGTTTTTTAGTATTTGGGGAG

mRNA sequence

GAAAAGGATTTGATGTATTGAACAATTTTTTCACTCAAAAATCCATTTTAGTATTCGCATGGAAAGTCCCTCTAATCACAGGATTTGAAATCGTTCCATAGCAGCAGGACATGGAGGAATCAGCCCAAGCTTTCAAGAATCTTCACAGCAGAGAGTATCAAGGTCACAAGAAGAAGGTACATTCTGTGGCATGGAATTGCACGGGCATGAAGCTTGCTTCCGGTTCTGTTGATCAAACTGCTCGAGTTTGGCATATTGAGCCTCACGGACATGGTAAGGTTAAGGATGTTGAGTTGAAAGGGCACACTGATAGTGTAGATCAGCTTTGCTGGGACCCTAAACATTCTGATCTTATAGCGACTGCATCTGGAGACAAGACTGTTCGACTATGGGATGCTCGCAATGGGAAATGCTCACAGCAAGCTGAGCTCAGTGGGGAAAATATCAACATCACCTACAAACCTGATGGGACACACATCGCTGTTGGAAATAGGGATGATGAACTTACAATTCTGGATGTTAGGAAGTTTAAACCAGTTCACAAGCGCAAGTTCAACTATGAGGTGAATGAAATTGCATGGAACATGACTGGGGAGATGTTTTTCCTGACAACTGGAAATGGTACTGTTGAAGTACTAGCATACCCATCGCTTCGACCAATCGAAACTCTTATGGCTCATACAGCGGGTTGTTACTGCATTGCGATTGACCCTGTTGGAGGGTATTTTGCAGTTGGAAGTGCTGATTCATTAGTTAGCCTATGGGATATATCGCAGATGCTTTGTGTGCGAACATTTACAAAACTTGAATGGCCTGTTAGAACAATAAGTTTCAACCACACAGGAGAATACATTGCTTCTGCCAGTGAGGATTTGTTCATTGATATATCAAATGTTCAAACGGGACGAACGGTTCATCAGATTCCTTGTCGGGCTGCAATGAACAGTGTGGAGTGGAATCCTAAACATAACTTACTTGCATATGCTGGGGATGACAAGAACAAGTATCAGGCTGATGAAGGTATTTTTAGGATCTTTGGGTTTGAAAGTCCATGAGAAATGGATCAACCATGGAGAAATTTCCAATTCTGTGATACTTTATTATGTTCCTTCATTTCTGGCAAAATGTATTGAATTTCAGTTAGTGGCTGTTACCATAATAACTTAGTCAAATTTTCCCTTGAAACAACAATCTGTGGTTGTATTACCTCTTGAAAAGTTCACCATGATAAAATCTTAACTTGAAACAACCATTCCTACACACCTGATGCTTAGAAGTTCCATCTTTTTAACTCGAGTTTTTTAGTATTTGGGGAG

Coding sequence (CDS)

ATGGAGGAATCAGCCCAAGCTTTCAAGAATCTTCACAGCAGAGAGTATCAAGGTCACAAGAAGAAGGTACATTCTGTGGCATGGAATTGCACGGGCATGAAGCTTGCTTCCGGTTCTGTTGATCAAACTGCTCGAGTTTGGCATATTGAGCCTCACGGACATGGTAAGGTTAAGGATGTTGAGTTGAAAGGGCACACTGATAGTGTAGATCAGCTTTGCTGGGACCCTAAACATTCTGATCTTATAGCGACTGCATCTGGAGACAAGACTGTTCGACTATGGGATGCTCGCAATGGGAAATGCTCACAGCAAGCTGAGCTCAGTGGGGAAAATATCAACATCACCTACAAACCTGATGGGACACACATCGCTGTTGGAAATAGGGATGATGAACTTACAATTCTGGATGTTAGGAAGTTTAAACCAGTTCACAAGCGCAAGTTCAACTATGAGGTGAATGAAATTGCATGGAACATGACTGGGGAGATGTTTTTCCTGACAACTGGAAATGGTACTGTTGAAGTACTAGCATACCCATCGCTTCGACCAATCGAAACTCTTATGGCTCATACAGCGGGTTGTTACTGCATTGCGATTGACCCTGTTGGAGGGTATTTTGCAGTTGGAAGTGCTGATTCATTAGTTAGCCTATGGGATATATCGCAGATGCTTTGTGTGCGAACATTTACAAAACTTGAATGGCCTGTTAGAACAATAAGTTTCAACCACACAGGAGAATACATTGCTTCTGCCAGTGAGGATTTGTTCATTGATATATCAAATGTTCAAACGGGACGAACGGTTCATCAGATTCCTTGTCGGGCTGCAATGAACAGTGTGGAGTGGAATCCTAAACATAACTTACTTGCATATGCTGGGGATGACAAGAACAAGTATCAGGCTGATGAAGGTATTTTTAGGATCTTTGGGTTTGAAAGTCCATGA

Protein sequence

MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQADEGIFRIFGFESP*

Homology

[Close]

BLAST of CSPI03G04360 vs. ExPASy Swiss-Prot
Match: Q9FKT5 (THO complex subunit 3 OS=Arabidopsis thaliana OX=3702 GN=THO3 PE=1 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 4.7e-166
Identity = 272/314 (86.62%), Postives = 295/314 (93.95%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+   FK+LHSREYQGHKKKVHSVAWN  G KLASGSVDQTAR+W+IEPHGH K KD+
Sbjct: 1   MEETTIPFKSLHSREYQGHKKKVHSVAWNSNGTKLASGSVDQTARIWNIEPHGHSKAKDL 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDL+ATASGDK+VRLWDAR+GKC+QQ ELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLVATASGDKSVRLWDARSGKCTQQVELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+H+RKFNYEVNEIAWNM G+ FFLTTG GTVEVL+YPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPLHRRKFNYEVNEIAWNMPGDFFFLTTGLGTVEVLSYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           L+P++TL AHTAGCYCIAIDP G YFAVGSADSLVSLWDIS MLC+RTFTKLEWPVRTIS
Sbjct: 181 LKPLDTLTAHTAGCYCIAIDPKGRYFAVGSADSLVSLWDISDMLCLRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KY 300
           FN++GEYIASASEDLFIDI+NVQTGRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKN KY
Sbjct: 241 FNYSGEYIASASEDLFIDIANVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNPKY 300

Query: 301 QADEGIFRIFGFES 314
             DEG+FRIFGFES
Sbjct: 301 NTDEGVFRIFGFES 314

[Close]

BLAST of CSPI03G04360 vs. ExPASy Swiss-Prot
Match: Q29RH4 (THO complex subunit 3 OS=Bos taurus OX=9913 GN=THOC3 PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 7.7e-92
Identity = 154/301 (51.16%), Postives = 212/301 (70.43%), Query Frame = 0


Query: 13  SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQL 72
           +RE+  H  KVHSVAW+C G +LASGS D+TA V+ +E      VK+   +GH DSVDQL
Sbjct: 48  TREFPAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEK--DRLVKENNYRGHGDSVDQL 107

Query: 73  CWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDEL 132
           CW P + DL  TASGDKT+R+WD R  KC       GENINI + PDG  IAVGN+DD +
Sbjct: 108 CWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVV 167

Query: 133 TILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTA 192
           T +D +  +   + +F +EVNEI+WN    MFFLT GNG + +L+YP L+P++++ AH +
Sbjct: 168 TFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPS 227

Query: 193 GCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASAS 252
            C CI  DP+G YFA GSAD+LVSLWD+ +++CVR F++L+WPVRT+SF+H G+ +ASAS
Sbjct: 228 NCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASAS 287

Query: 253 EDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KYQA--DEGIFRIF 311
           ED FIDI+ V+TG  + ++ C +   +V W+PK  LLA+A DDK+ KY +  + G  ++F
Sbjct: 288 EDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLF 346

[Close]

BLAST of CSPI03G04360 vs. ExPASy Swiss-Prot
Match: Q8VE80 (THO complex subunit 3 OS=Mus musculus OX=10090 GN=Thoc3 PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 7.7e-92
Identity = 154/301 (51.16%), Postives = 212/301 (70.43%), Query Frame = 0


Query: 13  SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQL 72
           +RE+  H  KVHSVAW+C G +LASGS D+TA V+ +E      VK+   +GH DSVDQL
Sbjct: 48  TREFPAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEK--DRLVKENNYRGHGDSVDQL 107

Query: 73  CWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDEL 132
           CW P + DL  TASGDKT+R+WD R  KC       GENINI + PDG  IAVGN+DD +
Sbjct: 108 CWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVV 167

Query: 133 TILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTA 192
           T +D +  +   + +F +EVNEI+WN    MFFLT GNG + +L+YP L+P++++ AH +
Sbjct: 168 TFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPS 227

Query: 193 GCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASAS 252
            C CI  DP+G YFA GSAD+LVSLWD+ +++CVR F++L+WPVRT+SF+H G+ +ASAS
Sbjct: 228 NCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASAS 287

Query: 253 EDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KYQA--DEGIFRIF 311
           ED FIDI+ V+TG  + ++ C +   +V W+PK  LLA+A DDK+ KY +  + G  ++F
Sbjct: 288 EDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLF 346

[Close]

BLAST of CSPI03G04360 vs. ExPASy Swiss-Prot
Match: Q96J01 (THO complex subunit 3 OS=Homo sapiens OX=9606 GN=THOC3 PE=1 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.0e-91
Identity = 154/301 (51.16%), Postives = 212/301 (70.43%), Query Frame = 0


Query: 13  SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQL 72
           +RE+  H  KVHSVAW+C G +LASGS D+TA V+ +E      VK+   +GH DSVDQL
Sbjct: 48  TREFLAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEK--DRLVKENNYRGHGDSVDQL 107

Query: 73  CWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDEL 132
           CW P + DL  TASGDKT+R+WD R  KC       GENINI + PDG  IAVGN+DD +
Sbjct: 108 CWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVV 167

Query: 133 TILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTA 192
           T +D +  +   + +F +EVNEI+WN    MFFLT GNG + +L+YP L+P++++ AH +
Sbjct: 168 TFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPS 227

Query: 193 GCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASAS 252
            C CI  DP+G YFA GSAD+LVSLWD+ +++CVR F++L+WPVRT+SF+H G+ +ASAS
Sbjct: 228 NCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASAS 287

Query: 253 EDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KYQA--DEGIFRIF 311
           ED FIDI+ V+TG  + ++ C +   +V W+PK  LLA+A DDK+ KY +  + G  ++F
Sbjct: 288 EDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLF 346

[Close]

BLAST of CSPI03G04360 vs. ExPASy Swiss-Prot
Match: P14197 (WD repeat-containing protein AAC3 OS=Dictyostelium discoideum OX=44689 GN=AAC3 PE=2 SV=2)

HSP 1 Score: 245.4 bits (625), Expect = 8.9e-64
Identity = 120/336 (35.71%), Postives = 199/336 (59.23%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHG------- 60
           ++ +++ F    ++++ G+KKK  SVAWN  G K+AS   D   RVW+ +P G       
Sbjct: 146 LDLASRYFSECSTKDFIGNKKKSTSVAWNANGTKIASSGSDGIVRVWNFDPLGNSNNNNN 205

Query: 61  ---------HGKVKD-VELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQ 120
                    +  +K+ +ELKGH  S++++ W PK++DL+A+A  DK +++WD + GKC  
Sbjct: 206 SNNTSSNSKNNNIKETIELKGHDGSIEKISWSPKNNDLLASAGTDKVIKIWDVKIGKCIG 265

Query: 121 QAELSGENINITYKPDGTHIAVGNRDDELTILDVRKFKPVHKRKFN-YEVNEIAWNMTGE 180
               + ENI++ + PDG  I    RDD L ++D+   K +   KFN  E+N++ W+  G+
Sbjct: 266 TVSTNSENIDVRWSPDGQFIVACTRDDHLALIDLPTIKTLKIYKFNGEELNQVGWDNNGD 325

Query: 181 MFFLTTGNGTVEVLAY-----PSLRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSL 240
           +  +    G +E   +       ++ ++TL  HTA  YC+  DP G Y A GSADS+VSL
Sbjct: 326 LILMANSMGNIEAYKFLPKSTTHVKHLKTLYGHTASIYCMEFDPTGKYLAAGSADSIVSL 385

Query: 241 WDISQMLCVRTFTKLEWPVRTISFNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAM 300
           WDI  M+CV+TF K  +P R++SF+  G++IA++S +  I+I ++++ + +H I C + +
Sbjct: 386 WDIEDMMCVKTFIKSTFPCRSVSFSFDGQFIAASSFESTIEIFHIESSQPIHTIEC-SGV 445

Query: 301 NSVEWNPKHNLLAYAGDDKNKYQADEGIFRIFGFES 314
           +S+ W+P   LLAYA  + N+   D  I R+FG+ S
Sbjct: 446 SSLMWHPTLPLLAYA-PEINENNKDPSI-RVFGYHS 478

[Close]

BLAST of CSPI03G04360 vs. ExPASy TrEMBL
Match: A0A0A0L651 (WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G061010 PE=4 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 6.6e-187
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESP 315
           ADEGIFRIFGFESP
Sbjct: 301 ADEGIFRIFGFESP 314

[Close]

BLAST of CSPI03G04360 vs. ExPASy TrEMBL
Match: A0A1S3BM38 (THO complex subunit 3 OS=Cucumis melo OX=3656 GN=LOC103491048 PE=4 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 6.6e-187
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESP 315
           ADEGIFRIFGFESP
Sbjct: 301 ADEGIFRIFGFESP 314

[Close]

BLAST of CSPI03G04360 vs. ExPASy TrEMBL
Match: A0A6J1KZA7 (THO complex subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111498453 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.4e-183
Identity = 308/313 (98.40%), Postives = 311/313 (99.36%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES  AFKNL+SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFES 314
           ADEGIFRIFGFES
Sbjct: 301 ADEGIFRIFGFES 313

[Close]

BLAST of CSPI03G04360 vs. ExPASy TrEMBL
Match: A0A6J1G4N9 (THO complex subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111450715 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.4e-183
Identity = 308/313 (98.40%), Postives = 311/313 (99.36%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES  AFKNL+SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFES 314
           ADEGIFRIFGFES
Sbjct: 301 ADEGIFRIFGFES 313

[Close]

BLAST of CSPI03G04360 vs. ExPASy TrEMBL
Match: A0A5D3BC11 (THO complex subunit 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001510 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.0e-180
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 204 MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 263

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 264 ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 323

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 324 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 383

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 384 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 443

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 444 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 503

Query: 301 ADEG 305
           ADEG
Sbjct: 504 ADEG 507

[Close]

BLAST of CSPI03G04360 vs. NCBI nr
Match: XP_004149572.1 (THO complex subunit 3 [Cucumis sativus] >XP_008449067.1 PREDICTED: THO complex subunit 3 [Cucumis melo] >KGN56047.1 hypothetical protein Csa_011347 [Cucumis sativus])

HSP 1 Score: 662.9 bits (1709), Expect = 1.4e-186
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESP 315
           ADEGIFRIFGFESP
Sbjct: 301 ADEGIFRIFGFESP 314

[Close]

BLAST of CSPI03G04360 vs. NCBI nr
Match: XP_038905145.1 (THO complex subunit 3 [Benincasa hispida])

HSP 1 Score: 654.1 bits (1686), Expect = 6.3e-184
Identity = 309/313 (98.72%), Postives = 311/313 (99.36%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEESA  FKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAPIFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKP+HKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPIHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFES 314
           ADEGIFRIFGFE+
Sbjct: 301 ADEGIFRIFGFET 313

[Close]

BLAST of CSPI03G04360 vs. NCBI nr
Match: XP_022946738.1 (THO complex subunit 3 [Cucurbita moschata] >XP_023005479.1 THO complex subunit 3 [Cucurbita maxima] >XP_023540495.1 THO complex subunit 3 [Cucurbita pepo subsp. pepo] >KAG6596748.1 THO complex subunit 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028285.1 THO complex subunit 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 650.2 bits (1676), Expect = 9.1e-183
Identity = 308/313 (98.40%), Postives = 311/313 (99.36%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES  AFKNL+SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFES 314
           ADEGIFRIFGFES
Sbjct: 301 ADEGIFRIFGFES 313

[Close]

BLAST of CSPI03G04360 vs. NCBI nr
Match: KAA0065764.1 (THO complex subunit 3 [Cucumis melo var. makuwa] >TYJ96504.1 THO complex subunit 3 [Cucumis melo var. makuwa])

HSP 1 Score: 641.3 bits (1653), Expect = 4.2e-180
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 204 MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 263

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 264 ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 323

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 324 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 383

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 384 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 443

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 444 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 503

Query: 301 ADEG 305
           ADEG
Sbjct: 504 ADEG 507

[Close]

BLAST of CSPI03G04360 vs. NCBI nr
Match: XP_022155009.1 (THO complex subunit 3 isoform X2 [Momordica charantia])

HSP 1 Score: 639.8 bits (1649), Expect = 1.2e-179
Identity = 301/313 (96.17%), Postives = 308/313 (98.40%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+A  FKNL SREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEEAALPFKNLQSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDAR+GKCSQQ ELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARSGKCSQQVELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+HKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPIHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP+ETLMAHTAGCYCIAIDPVG YFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPLETLMAHTAGCYCIAIDPVGAYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FN+TG+YIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNYTGKYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFES 314
           ADEGIFRIFGFES
Sbjct: 301 ADEGIFRIFGFES 313

[Close]

BLAST of CSPI03G04360 vs. TAIR 10
Match: AT5G56130.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 585.1 bits (1507), Expect = 3.4e-167
Identity = 272/314 (86.62%), Postives = 295/314 (93.95%), Query Frame = 0


Query: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+   FK+LHSREYQGHKKKVHSVAWN  G KLASGSVDQTAR+W+IEPHGH K KD+
Sbjct: 1   MEETTIPFKSLHSREYQGHKKKVHSVAWNSNGTKLASGSVDQTARIWNIEPHGHSKAKDL 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDL+ATASGDK+VRLWDAR+GKC+QQ ELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLVATASGDKSVRLWDARSGKCTQQVELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+H+RKFNYEVNEIAWNM G+ FFLTTG GTVEVL+YPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPLHRRKFNYEVNEIAWNMPGDFFFLTTGLGTVEVLSYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           L+P++TL AHTAGCYCIAIDP G YFAVGSADSLVSLWDIS MLC+RTFTKLEWPVRTIS
Sbjct: 181 LKPLDTLTAHTAGCYCIAIDPKGRYFAVGSADSLVSLWDISDMLCLRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KY 300
           FN++GEYIASASEDLFIDI+NVQTGRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKN KY
Sbjct: 241 FNYSGEYIASASEDLFIDIANVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNPKY 300

Query: 301 QADEGIFRIFGFES 314
             DEG+FRIFGFES
Sbjct: 301 NTDEGVFRIFGFES 314

[Close]

BLAST of CSPI03G04360 vs. TAIR 10
Match: AT5G67320.1 (WD-40 repeat family protein )

HSP 1 Score: 106.3 bits (264), Expect = 4.6e-23
Identity = 82/293 (27.99%), Postives = 133/293 (45.39%), Query Frame = 0


Query: 12  HSREYQGHKKK-VHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVD 71
           H++     K K V ++ WN  G  LA+GS D  AR+W +    +G++    L  H   + 
Sbjct: 315 HAKGKSNEKSKDVTTLDWNGEGTLLATGSCDGQARIWTL----NGELIST-LSKHKGPIF 374

Query: 72  QLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAEL-SGENINITYKPDGTHIAVGNRD 131
            L W+ K  D + T S D+T  +WD +  +  QQ E  SG  +++ ++ +    A  + D
Sbjct: 375 SLKWN-KKGDYLLTGSVDRTAVVWDVKAEEWKQQFEFHSGPTLDVDWR-NNVSFATSSTD 434

Query: 132 DELTILDVRKFKPVHK-RKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLM 191
             + +  + + +P         EVN + W+ TG +    + + T ++        +  L 
Sbjct: 435 SMIYLCKIGETRPAKTFTGHQGEVNCVKWDPTGSLLASCSDDSTAKIWNIKQSTFVHDLR 494

Query: 192 AHTAGCYCIAIDPVGG---------YFAVGSADSLVSLWD--ISQMLCVRTFTKLEWPVR 251
            HT   Y I   P G            A  S DS V LWD  + +MLC  +F     PV 
Sbjct: 495 EHTKEIYTIRWSPTGPGTNNPNKQLTLASASFDSTVKLWDAELGKMLC--SFNGHREPVY 554

Query: 252 TISFNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLA 291
           +++F+  GEYIAS S D  I I +++ G+ V        +  V WN + N +A
Sbjct: 555 SLAFSPNGEYIASGSLDKSIHIWSIKEGKIVKTYTGNGGIFEVCWNKEGNKIA 598

[Close]

BLAST of CSPI03G04360 vs. TAIR 10
Match: AT3G49660.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 1.9e-16
Identity = 72/300 (24.00%), Postives = 131/300 (43.67%), Query Frame = 0


Query: 11  LHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV-ELKGHTDSV 70
           +HS+    H + V SV ++  G  LAS S D+T R + I        + V E  GH + +
Sbjct: 15  VHSQTLTSHNRAVSSVKFSSDGRLLASASADKTIRTYTINTINDPIAEPVQEFTGHENGI 74

Query: 71  DQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGEN---INITYKPDGTHIAVG 130
             + +    +  I +AS DKT++LWD   G  S    L G       + + P    I  G
Sbjct: 75  SDVAFS-SDARFIVSASDDKTLKLWDVETG--SLIKTLIGHTNYAFCVNFNPQSNMIVSG 134

Query: 131 NRDDELTILDVR-----KFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSL 190
           + D+ + I DV      K  P H    +  V  + +N  G +   ++ +G   +    + 
Sbjct: 135 SFDETVRIWDVTTGKCLKVLPAH----SDPVTAVDFNRDGSLIVSSSYDGLCRIWDSGTG 194

Query: 191 RPIETLM-AHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFT---KLEWPVR 250
             ++TL+         +   P G +  VG+ D+ + LW+IS    ++T+T     ++ + 
Sbjct: 195 HCVKTLIDDENPPVSFVRFSPNGKFILVGTLDNTLRLWNISSAKFLKTYTGHVNAQYCIS 254

Query: 251 TISFNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRA-AMNSVEWNPKHNLLAYAGDDK 297
           +      G+ I S SED  + +  + + + + ++      + +V  +P  NL+A    DK
Sbjct: 255 SAFSVTNGKRIVSGSEDNCVHMWELNSKKLLQKLEGHTETVMNVACHPTENLIASGSLDK 307

[Close]

BLAST of CSPI03G04360 vs. TAIR 10
Match: AT5G25150.1 (TBP-associated factor 5 )

HSP 1 Score: 80.5 bits (197), Expect = 2.7e-15
Identity = 64/257 (24.90%), Postives = 104/257 (40.47%), Query Frame = 0


Query: 16  YQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDVE-LKGHTDSVDQLCW 75
           Y+GH   V    ++  G   AS S D+TAR+W ++     +++ +  + GH   VD + W
Sbjct: 456 YKGHNYPVWDAQFSPFGHYFASCSHDRTARIWSMD-----RIQPLRIMAGHLSDVDCVQW 515

Query: 76  DPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGEN---INITYKPDGTHIAVGNRDDE 135
            P + + IAT S DKTVRLWD + G+C +     G     +++   PDG ++A G+ D  
Sbjct: 516 HP-NCNYIATGSSDKTVRLWDVQTGECVR--IFIGHRSMVLSLAMSPDGRYMASGDED-- 575

Query: 136 LTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHT 195
                                                  GT+ +    + R I  LM H 
Sbjct: 576 ---------------------------------------GTIMMWDLSTARCITPLMGHN 635

Query: 196 AGCYCIAIDPVGGYFAVGSADSLVSLWDI----------------SQMLCVRTFTKLEWP 253
           +  + ++    G   A GSAD  V LWD+                +++  +RTF     P
Sbjct: 636 SCVWSLSYSGEGSLLASGSADCTVKLWDVTSSTKLTKAEEKNGNSNRLRSLRTFPTKSTP 663

[Close]

BLAST of CSPI03G04360 vs. TAIR 10
Match: AT5G08390.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.9e-13
Identity = 37/160 (23.12%), Postives = 74/160 (46.25%), Query Frame = 0


Query: 152 VNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTAGCYCIAIDPVGGYFAVGSA 211
           ++ + ++ +  +      +GT+++      + + TL  H + C  +   P G +FA GS 
Sbjct: 62  IDSVTFDASEGLVAAGAASGTIKLWDLEEAKVVRTLTGHRSNCVSVNFHPFGEFFASGSL 121

Query: 212 DSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASASEDLFIDISNVQTGRTVHQI 271
           D+ + +WDI +  C+ T+      V  + F   G +I S  ED  + + ++  G+ +H+ 
Sbjct: 122 DTNLKIWDIRKKGCIHTYKGHTRGVNVLRFTPDGRWIVSGGEDNVVKVWDLTAGKLLHEF 181

Query: 272 PC-RAAMNSVEWNPKHNLLAYAGDDKNKYQADEGIFRIFG 311
                 + S++++P   LLA    DK     D   F + G
Sbjct: 182 KSHEGKIQSLDFHPHEFLLATGSADKTVKFWDLETFELIG 221

The following BLAST results are available for this feature:

BLAST of CSPI03G04360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKT5	4.7e-166	86.62	THO complex subunit 3 OS=Arabidopsis thaliana OX=3702 GN=THO3 PE=1 SV=1	[more]
Q29RH4	7.7e-92	51.16	THO complex subunit 3 OS=Bos taurus OX=9913 GN=THOC3 PE=2 SV=1	[more]
Q8VE80	7.7e-92	51.16	THO complex subunit 3 OS=Mus musculus OX=10090 GN=Thoc3 PE=2 SV=1	[more]
Q96J01	1.0e-91	51.16	THO complex subunit 3 OS=Homo sapiens OX=9606 GN=THOC3 PE=1 SV=1	[more]
P14197	8.9e-64	35.71	WD repeat-containing protein AAC3 OS=Dictyostelium discoideum OX=44689 GN=AAC3 P...	[more]

BLAST of CSPI03G04360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L651	6.6e-187	100.00	WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A1S3BM38	6.6e-187	100.00	THO complex subunit 3 OS=Cucumis melo OX=3656 GN=LOC103491048 PE=4 SV=1	[more]
A0A6J1KZA7	4.4e-183	98.40	THO complex subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111498453 PE=4 SV=1	[more]
A0A6J1G4N9	4.4e-183	98.40	THO complex subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111450715 PE=4 SV=1	[more]
A0A5D3BC11	2.0e-180	100.00	THO complex subunit 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold54...	[more]

BLAST of CSPI03G04360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149572.1	1.4e-186	100.00	THO complex subunit 3 [Cucumis sativus] >XP_008449067.1 PREDICTED: THO complex s...	[more]
XP_038905145.1	6.3e-184	98.72	THO complex subunit 3 [Benincasa hispida]	[more]
XP_022946738.1	9.1e-183	98.40	THO complex subunit 3 [Cucurbita moschata] >XP_023005479.1 THO complex subunit 3...	[more]
KAA0065764.1	4.2e-180	100.00	THO complex subunit 3 [Cucumis melo var. makuwa] >TYJ96504.1 THO complex subunit...	[more]
XP_022155009.1	1.2e-179	96.17	THO complex subunit 3 isoform X2 [Momordica charantia]	[more]

BLAST of CSPI03G04360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56130.1	3.4e-167	86.62	Transducin/WD40 repeat-like superfamily protein	[more]
AT5G67320.1	4.6e-23	27.99	WD-40 repeat family protein	[more]
AT3G49660.1	1.9e-16	24.00	Transducin/WD40 repeat-like superfamily protein	[more]
AT5G25150.1	2.7e-15	24.90	TBP-associated factor 5	[more]
AT5G08390.1	1.9e-13	23.13	Transducin/WD40 repeat-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020472	G-protein beta WD-40 repeat	PRINTS	PR00320	GPROTEINBRPT	coord: 50..64 score: 43.08 coord: 174..188 score: 33.56 coord: 3..17 score: 38.84
IPR001680	WD40 repeat	SMART	SM00320	WD40_4	coord: 148..187 e-value: 6.7E-8 score: 42.3 coord: 190..229 e-value: 1.5 score: 15.9 coord: 23..63 e-value: 2.6E-8 score: 43.6 coord: 232..270 e-value: 48.0 score: 6.4 coord: 107..145 e-value: 150.0 score: 3.3
IPR001680	WD40 repeat	PFAM	PF00400	WD40	coord: 151..187 e-value: 0.021 score: 15.7 coord: 29..63 e-value: 2.3E-4 score: 21.9
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 155..196 score: 12.01227
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 1..18 score: 8.503359
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 30..72 score: 15.554599
IPR024977	Anaphase-promoting complex subunit 4, WD40 domain	PFAM	PF12894	ANAPC4_WD40	coord: 202..251 e-value: 1.5E-4 score: 21.9
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 1..115 e-value: 2.1E-24 score: 88.4
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 116..278 e-value: 1.6E-29 score: 104.9
IPR040132	TREX component Tex1/THOC3	PANTHER	PTHR22839	THO COMPLEX SUBUNIT 3 THO3	coord: 2..280
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 50..64
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 155..189 score: 9.54651
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 30..68 score: 12.103769
IPR036322	WD40-repeat-containing domain superfamily	SUPERFAMILY	50978	WD40 repeat-like	coord: 3..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G04360.1	CSPI03G04360.1	mRNA
CSPI03G04360.2	CSPI03G04360.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006406	mRNA export from nucleus
biological_process	GO:0010267	production of ta-siRNAs involved in RNA interference
cellular_component	GO:0000445	THO complex part of transcription export complex
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0005515	protein binding