CSPI03G04320 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G04320
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr3: 3530546 .. 3531743 (+)
RNA-Seq Expression	CSPI03G04320
Synteny	CSPI03G04320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTGGTCTATGGACCAACCAGGCAGCCCATAAAGCTCACCGGAAAACTGTATAGATAGAGGAGTTCTAGAAGACTTCATAACTCCTGAAGTTGACCACCCTCGAAGATTGAAATTTATTTAAAGATACAAGCTTTCTTCTAAGACTTCAACTTCAAAAACATCACATGTTTCGCTTCCTCAAATCAAGCGTAGATATTCAATCAACTTCGAGAACATCACGTGCTCCGCTTCCTCAAATCAAGCATAAGCGTTCAATCAACTTTGAGAACATCGCGTGCTCTATTTCCTCAAAATCAAGTGCATGCGTTCATTCAACTTCAAGTATATCCTAAAGCATTGTACATGGTTGATCTGGAAGTAATTGACAATAATTATGCCTCTTCTAAAGATGTCGACAATTCAACTAAAACTAAGCAAAGGACTTTCGTCTTTGATCGTATCAGACCTTCAACTACTCGATTTTCAACCTTTCAAAGAATGCGTATGACCACGAAAGAAGAAGAAAACTAATGTCCAACCTCTACTTACACTCAAACTTCAACTTTCAAAAAGCTAAGTATCTCCACATCGAAGAAAAGTTGACCTTCGACATTTGCTTTTGATCGTCTAAAGATGAGAGGTAATCAACATGAAAGAGATATGAAAACATTAAATGCAAAATTATTTCATGAAGAAAACAATAACAATAAAATTCACAGTCGTGTCCCATCACGCATGAAGAGGAAATTGTCTATTGACATAAATACATAAGATTCCTTGACAGCGAAGACAATACTTATTATATTTGCCAATTCTAAAAACGAAGGAGATAAACAAATCCTTGATGAAAATATGAGTTATCGAACTTGCATGCTTAATGATTCTTGTACAGATGAATGTGAAGGGAAATATTGTCATTTGTATAATCAGTTATTCTTTTTGAAGTTGATGGGGGAAGCCTCAGATGTTGCGGGATGTTTCAACACGAGAGTTAGACATCTTCTTCATCTTCACGTCGCTGGAGGAATTGAGTTGAGAGATACATGATGCGATTGGTTCATTGCTTGAAAATTGATCAAACCACGTTGATACAAGAAGGTCGAATGTTGATTGAATATGTTACAATGGATGTTATTGCTATCATAAAGATTCTCAATAAATATGATAAGGTACATAGCTCTGTAAATGGAAAGAACTTTAAGTTGAAAATGTG

mRNA sequence

ATGGGTTTGGTCTATGGACCAACCAGGCAGCCCATAAAGCTCACCGGAAAACTCATAAGCGTTCAATCAACTTTGAGAACATCGCGTGCTCTATTTCCTCAAAATCAAGTGCATGCGTTCATTCAACTTCAAGTATATCCTAAAGCATTGTACATGGTTGATCTGGAAGTAATTGACAATAATTATGCCTCTTCTAAAGATGTCGACAATTCAACTAAAACTAAGCAAAGGACTTTCGTCTTTGATCGTATCAGACCTTCAACTACTCGATTTTCAACCTTTCAAAGAATGCATGAATGTGAAGGGAAATATTGTCATTTGTATAATCAGTTATTCTTTTTGAAGTTGATGGGGGAAGCCTCAGATGTTGCGGGATGTTTCAACACGAGAGTTAGACATCTTCTTCATCTTCACGTCGCTGGAGGAATTGAGTTGAGAGATACATGATGCGATTGGTTCATTGCTTGAAAATTGATCAAACCACGTTGATACAAGAAGGTCGAATGTTGATTGAATATGTTACAATGGATGTTATTGCTATCATAAAGATTCTCAATAAATATGATAAGGTACATAGCTCTGTAAATGGAAAGAACTTTAAGTTGAAAATGTG

Coding sequence (CDS)

ATGGGTTTGGTCTATGGACCAACCAGGCAGCCCATAAAGCTCACCGGAAAACTCATAAGCGTTCAATCAACTTTGAGAACATCGCGTGCTCTATTTCCTCAAAATCAAGTGCATGCGTTCATTCAACTTCAAGTATATCCTAAAGCATTGTACATGGTTGATCTGGAAGTAATTGACAATAATTATGCCTCTTCTAAAGATGTCGACAATTCAACTAAAACTAAGCAAAGGACTTTCGTCTTTGATCGTATCAGACCTTCAACTACTCGATTTTCAACCTTTCAAAGAATGCATGAATGTGAAGGGAAATATTGTCATTTGTATAATCAGTTATTCTTTTTGAAGTTGATGGGGGAAGCCTCAGATGTTGCGGGATGTTTCAACACGAGAGTTAGACATCTTCTTCATCTTCACGTCGCTGGAGGAATTGAGTTGAGAGATACATGA

Protein sequence

MGLVYGPTRQPIKLTGKLISVQSTLRTSRALFPQNQVHAFIQLQVYPKALYMVDLEVIDNNYASSKDVDNSTKTKQRTFVFDRIRPSTTRFSTFQRMHECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIELRDT*

Homology

[Close]

BLAST of CSPI03G04320 vs. ExPASy Swiss-Prot
Match: Q8GW10 (Probable E3 ubiquitin-protein ligase BAH1-like OS=Arabidopsis thaliana OX=3702 GN=RF178 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 6.6e-09
Identity = 39/84 (46.43%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 62  YASSKDVDNSTKTKQRTFVFD-RIRPSTTRFSTFQRMHECEGKYCHLYNQLFFLKLMGEA 121
           Y   K V    KT   T   D +I PS T  S       CE K C   +Q+FF +LM EA
Sbjct: 27  YKKLKKVLKKCKTCNSTKSDDGQIIPSATSSSL---SDSCECKACPWCDQMFFEELMKEA 86

Query: 122 SDVAGCFNTRVRHLLHLHVAGGIE 145
           +D+AG F +RVRHLLHLHVA G++
Sbjct: 87  TDIAGFFRSRVRHLLHLHVATGMQ 107

[Close]

BLAST of CSPI03G04320 vs. ExPASy Swiss-Prot
Match: A2XK56 (Probable E3 ubiquitin-protein ligase BAH1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_12825 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.0e-06
Identity = 22/43 (51.16%), Postives = 32/43 (74.42%), Query Frame = 0


Query: 100 CEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGG 143
           C+   C L +Q+FF +L  EAS++AGCF++RV+ LL+LHV  G
Sbjct: 63  CKCNSCTLCDQMFFTELTKEASEIAGCFSSRVQRLLNLHVPSG 105

[Close]

BLAST of CSPI03G04320 vs. ExPASy Swiss-Prot
Match: Q7XZZ3 (Probable E3 ubiquitin-protein ligase BAH1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0650900 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.0e-06
Identity = 22/43 (51.16%), Postives = 32/43 (74.42%), Query Frame = 0


Query: 100 CEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGG 143
           C+   C L +Q+FF +L  EAS++AGCF++RV+ LL+LHV  G
Sbjct: 63  CKCNSCTLCDQMFFTELTKEASEIAGCFSSRVQRLLNLHVPSG 105

[Close]

BLAST of CSPI03G04320 vs. ExPASy TrEMBL
Match: A0A1S3B703 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486720 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-12
Identity = 37/46 (80.43%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 99  ECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           ECE K C L +QLFF +LM EASD+AGCFNTRVRHLLHLHVAGGIE
Sbjct: 49  ECEWKSCPLCDQLFFSELMREASDIAGCFNTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. ExPASy TrEMBL
Match: A0A5D3DPA7 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005010 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-12
Identity = 37/46 (80.43%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 99  ECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           ECE K C L +QLFF +LM EASD+AGCFNTRVRHLLHLHVAGGIE
Sbjct: 49  ECEWKSCPLCDQLFFSELMREASDIAGCFNTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. ExPASy TrEMBL
Match: A0A1S3B726 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486720 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-12
Identity = 37/46 (80.43%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 99  ECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           ECE K C L +QLFF +LM EASD+AGCFNTRVRHLLHLHVAGGIE
Sbjct: 49  ECEWKSCPLCDQLFFSELMREASDIAGCFNTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. ExPASy TrEMBL
Match: A0A0A0LBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G790460 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 6.6e-12
Identity = 36/47 (76.60%), Postives = 41/47 (87.23%), Query Frame = 0


Query: 98  HECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           +ECE K C + +QLFF +LM EASD+AGCFNTRVRHLLHLHVAGGIE
Sbjct: 48  NECECKSCPVCDQLFFSELMREASDIAGCFNTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. ExPASy TrEMBL
Match: A0A6J1J6F2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481655 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 5.6e-11
Identity = 35/47 (74.47%), Postives = 40/47 (85.11%), Query Frame = 0


Query: 98  HECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           +ECE K C L +QLFF +LM EA D+AGCF+TRVRHLLHLHVAGGIE
Sbjct: 48  NECECKSCPLCDQLFFSELMREALDIAGCFSTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. NCBI nr
Match: XP_008442986.1 (PREDICTED: probable E3 ubiquitin-protein ligase BAH1-like isoform X2 [Cucumis melo] >KAA0043771.1 putative E3 ubiquitin-protein ligase BAH1-like isoform X2 [Cucumis melo var. makuwa] >TYK25362.1 putative E3 ubiquitin-protein ligase BAH1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 81.6 bits (200), Expect = 6.1e-12
Identity = 37/46 (80.43%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 99  ECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           ECE K C L +QLFF +LM EASD+AGCFNTRVRHLLHLHVAGGIE
Sbjct: 49  ECEWKSCPLCDQLFFSELMREASDIAGCFNTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. NCBI nr
Match: XP_008442983.1 (PREDICTED: probable E3 ubiquitin-protein ligase BAH1-like isoform X1 [Cucumis melo] >XP_008442985.1 PREDICTED: probable E3 ubiquitin-protein ligase BAH1-like isoform X1 [Cucumis melo])

HSP 1 Score: 81.6 bits (200), Expect = 6.1e-12
Identity = 37/46 (80.43%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 99  ECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           ECE K C L +QLFF +LM EASD+AGCFNTRVRHLLHLHVAGGIE
Sbjct: 49  ECEWKSCPLCDQLFFSELMREASDIAGCFNTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. NCBI nr
Match: XP_031739330.1 (probable E3 ubiquitin-protein ligase BAH1-like isoform X2 [Cucumis sativus] >KGN59253.1 hypothetical protein Csa_001320 [Cucumis sativus])

HSP 1 Score: 80.5 bits (197), Expect = 1.4e-11
Identity = 36/47 (76.60%), Postives = 41/47 (87.23%), Query Frame = 0


Query: 98  HECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           +ECE K C + +QLFF +LM EASD+AGCFNTRVRHLLHLHVAGGIE
Sbjct: 48  NECECKSCPVCDQLFFSELMREASDIAGCFNTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. NCBI nr
Match: XP_004136542.2 (probable E3 ubiquitin-protein ligase BAH1-like isoform X1 [Cucumis sativus])

HSP 1 Score: 80.5 bits (197), Expect = 1.4e-11
Identity = 36/47 (76.60%), Postives = 41/47 (87.23%), Query Frame = 0


Query: 98  HECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           +ECE K C + +QLFF +LM EASD+AGCFNTRVRHLLHLHVAGGIE
Sbjct: 48  NECECKSCPVCDQLFFSELMREASDIAGCFNTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. NCBI nr
Match: XP_038903375.1 (LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase BAH1-like [Benincasa hispida])

HSP 1 Score: 80.1 bits (196), Expect = 1.8e-11
Identity = 36/47 (76.60%), Postives = 41/47 (87.23%), Query Frame = 0


Query: 98  HECEGKYCHLYNQLFFLKLMGEASDVAGCFNTRVRHLLHLHVAGGIE 145
           +ECE K C L +QLFF +LM EASD+AGCF+TRVRHLLHLHVAGGIE
Sbjct: 48  NECEWKSCPLCDQLFFSELMREASDIAGCFSTRVRHLLHLHVAGGIE 94

[Close]

BLAST of CSPI03G04320 vs. TAIR 10
Match: AT2G38920.1 (SPX (SYG1/Pho81/XPR1) domain-containing protein / zinc finger (C3HC4-type RING finger) protein-related )

HSP 1 Score: 62.0 bits (149), Expect = 4.7e-10
Identity = 39/84 (46.43%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 62  YASSKDVDNSTKTKQRTFVFD-RIRPSTTRFSTFQRMHECEGKYCHLYNQLFFLKLMGEA 121
           Y   K V    KT   T   D +I PS T  S       CE K C   +Q+FF +LM EA
Sbjct: 27  YKKLKKVLKKCKTCNSTKSDDGQIIPSATSSSL---SDSCECKACPWCDQMFFEELMKEA 86

Query: 122 SDVAGCFNTRVRHLLHLHVAGGIE 145
           +D+AG F +RVRHLLHLHVA G++
Sbjct: 87  TDIAGFFRSRVRHLLHLHVATGMQ 107

[Close]

BLAST of CSPI03G04320 vs. TAIR 10
Match: AT2G38920.2 (SPX (SYG1/Pho81/XPR1) domain-containing protein / zinc finger (C3HC4-type RING finger) protein-related )

HSP 1 Score: 62.0 bits (149), Expect = 4.7e-10
Identity = 39/84 (46.43%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 62  YASSKDVDNSTKTKQRTFVFD-RIRPSTTRFSTFQRMHECEGKYCHLYNQLFFLKLMGEA 121
           Y   K V    KT   T   D +I PS T  S       CE K C   +Q+FF +LM EA
Sbjct: 27  YKKLKKVLKKCKTCNSTKSDDGQIIPSATSSSL---SDSCECKACPWCDQMFFEELMKEA 86

Query: 122 SDVAGCFNTRVRHLLHLHVAGGIE 145
           +D+AG F +RVRHLLHLHVA G++
Sbjct: 87  TDIAGFFRSRVRHLLHLHVATGMQ 107

[Close]

BLAST of CSPI03G04320 vs. TAIR 10
Match: AT2G38920.3 (SPX (SYG1/Pho81/XPR1) domain-containing protein / zinc finger (C3HC4-type RING finger) protein-related )

HSP 1 Score: 62.0 bits (149), Expect = 4.7e-10
Identity = 39/84 (46.43%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 62  YASSKDVDNSTKTKQRTFVFD-RIRPSTTRFSTFQRMHECEGKYCHLYNQLFFLKLMGEA 121
           Y   K V    KT   T   D +I PS T  S       CE K C   +Q+FF +LM EA
Sbjct: 27  YKKLKKVLKKCKTCNSTKSDDGQIIPSATSSSL---SDSCECKACPWCDQMFFEELMKEA 86

Query: 122 SDVAGCFNTRVRHLLHLHVAGGIE 145
           +D+AG F +RVRHLLHLHVA G++
Sbjct: 87  TDIAGFFRSRVRHLLHLHVATGMQ 107

The following BLAST results are available for this feature:

BLAST of CSPI03G04320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GW10	6.6e-09	46.43	Probable E3 ubiquitin-protein ligase BAH1-like OS=Arabidopsis thaliana OX=3702 G...	[more]
A2XK56	4.0e-06	51.16	Probable E3 ubiquitin-protein ligase BAH1-like 1 OS=Oryza sativa subsp. indica O...	[more]
Q7XZZ3	4.0e-06	51.16	Probable E3 ubiquitin-protein ligase BAH1-like 1 OS=Oryza sativa subsp. japonica...	[more]

BLAST of CSPI03G04320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B703	3.0e-12	80.43	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486720 PE=4 ...	[more]
A0A5D3DPA7	3.0e-12	80.43	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B726	3.0e-12	80.43	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486720 PE=4 ...	[more]
A0A0A0LBP6	6.6e-12	76.60	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G790460 PE...	[more]
A0A6J1J6F2	5.6e-11	74.47	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481655 P...	[more]

BLAST of CSPI03G04320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442986.1	6.1e-12	80.43	PREDICTED: probable E3 ubiquitin-protein ligase BAH1-like isoform X2 [Cucumis me...	[more]
XP_008442983.1	6.1e-12	80.43	PREDICTED: probable E3 ubiquitin-protein ligase BAH1-like isoform X1 [Cucumis me...	[more]
XP_031739330.1	1.4e-11	76.60	probable E3 ubiquitin-protein ligase BAH1-like isoform X2 [Cucumis sativus] >KGN...	[more]
XP_004136542.2	1.4e-11	76.60	probable E3 ubiquitin-protein ligase BAH1-like isoform X1 [Cucumis sativus]	[more]
XP_038903375.1	1.8e-11	76.60	LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase BAH1-like [Benincasa h...	[more]

BLAST of CSPI03G04320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38920.1	4.7e-10	46.43	SPX (SYG1/Pho81/XPR1) domain-containing protein / zinc finger (C3HC4-type RING f...	[more]
AT2G38920.2	4.7e-10	46.43	SPX (SYG1/Pho81/XPR1) domain-containing protein / zinc finger (C3HC4-type RING f...	[more]
AT2G38920.3	4.7e-10	46.43	SPX (SYG1/Pho81/XPR1) domain-containing protein / zinc finger (C3HC4-type RING f...	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G04320.1	CSPI03G04320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity