CSPI03G04130 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G04130
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptioncoiled-coil domain-containing protein 22
LocationChr3: 3379015 .. 3382592 (+)
RNA-Seq ExpressionCSPI03G04130
SyntenyCSPI03G04130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGATATATAATTTTTTGCAATTTGATGAAAAAAAGCCAAAGAAAAATCCATTTTCGGGTGTTTGATTTGTTCAGCAAGAAGTGTTAAGAACGAGAAGGAAAACGGCAAAAGTTCAACCGGAGCAATGGAGGAATCTCAGGAAATTCTTCTCAATTCTCTCCAAAAATCAGGTGTTTCAATCCCAACAGACCTTTCCTCGATTCGGAACTTATCCCCTCCCATATTCTTCTCCATCTGTGCTCAAGTTTTGAATCGAATACATCCCCAAACTAAAACCCCCTTCTCCACTTCTCTTCCGGACTCTGTCGCCGATCAATTCAAGGTATCTTCTGAAATCGCTTCCGCCATTAAGGATCTGGGTTTCATCGGAGATATTAGCTTCCACAAGGTATCCCACTACTTCCTTTTTTTCACCAACTAATTTTGTTGCTGTTTATATTTGGTTCGTTTATCAATTTACTCAGCTCTGATTTTGGTACATTTTCCATGTTAGCATTCTATTTCTGTTCATGGTGATCGTAGTGCAGCTTAAATTTTAAGGAGAGTGATAAATTTATAATCTAAACTTCGTATTTCTTTTGTACCTCTTGGACATTATTAGCAAAAGTTAAGGCACCCCATTTGATCATCATTTTGTTTTTGGTTTCAATTATGCTTGTTTTCTCACCCTAAACATGTTGGATTTTCAAGAACTAAATCAAGATTTTACAAACTAGTTTTTTTAGTTTTGAAAATTTGGGGTGGTTTTGAAAACGGTTTTAAAAAGTAAGTGATAAAGCAAAAAAACTGTATGTGGAAGTAGTGAATCTGAGGTTAATAGACTTTTTTGGAATTGTACTTAGAAACAGACTACATGTGTTGCTTACAACAGTTCTTGTATCCATCTGAAGAGGACTTGTACAAGCTGATAAGATTCTTGGTGGAGAGGCTTCCTGAAGCTTCAGATGGAAAATCTAAGGTTTTGGAAGATGGTGATGGTAGGAAAGAAACAAAAGCCGACACTTCCAAGGGCTACTATGTGGAGAACCAATCAAACAAGATCGATACTGACGATTCCGTTTCTACCCATCAGAAGGTTGAGAACAAACTTGCTGATCTCAATATTGTTGCTGAAGAGACAAAATCTCCAGATTCCATCGTTGATAGATTCTCTGATTTCCATCTCGACCGAAAAAGTTCGGGTGAAGCAGCTATGGTCGATAATCTTGTTAACGCTCCCAAGGATCAGTCTGAGGTGTCTGGAAATGAATCAGTTGAACTGCAGGGGAATAATAGACACGAAGTTGGTACTTACATTCAGAAGACCTTTGAAGTAAGTACATATGTTTCTTAACTTCAAACAATTATTAAAATCTAGATTTAGACATCAACTAGACATATAAGAGAACTAAATGGTCTTCCCCTTGGAAAAGGGTATTAGATCAGATGATATAACGAATATATGATATCTTCTTTTTTCGAATGAGAACTGAGACTAAGGAATTTCAGGATCAAGAGATCGGACCATTGGAAGAGGTCACTACAAATGCTTCTGAACTAAAGCATTTTCAAGTGGAGTTGGAAAGGTTGAAGGCAGTAACCAGTATTGTATTTGATGACAACCATTCGATTGAATTTCATCTTCGGCAGCTGGAGGAGCAAATAAATTCTAGAAAACTGGATCTTCTTGAAATGAAGTCACAGTGGTGGGGCATCATTTCAGTTTGATGGCTGGATTAACTTTTATACTACTGCTTTGGCATTATATTTAGTTTAAAAACCGAAGTCATGTAGATATTAAATTCTAATACAAAGATGTAGGTTTCATTTGTTTTTTTCTTTATCATTGATCTCTACAGGGATGCAGAGAGGGAGCTTTTAGAAATTAAAAGGCGAAGTCTACAAGAATCACTATGTGCAAGTAATCCAAAGGCTCAAGAAAAGCTGCAAAAATTGAGGGAGTTTGAATCGGAAAAGAAGCTCATTGAGTTGGAAATCAGGAGGCGGTATGCCACTTTCTTTTAATATTAAATAGGAAGTGAATCCTTACTGAAATCTCTGAAATATTCTCAGTATTTCTAGTAATAGTTTTTCAAAATGAACTACTGTCACATGAAATAGAAGTTTTAGGTTAGAAGTTATTTGGAGAATACCCTCCACGTCTTAGTTATAAATTGAGTTTTTCTTTACTTTTGAAGAGTGAAAAGTACTTTTAGCTAATTAAAAATTACTTTTAAAATTTATTTCGGTATTTTATTACATTAACGAAAATGCTTTTATAAAATGAAAAGGTAAGCACTAGTCCTTAGAAGTACTTAACTGGTTTGCATATTTGTCAAAAATCATCTTGAACTTACCATCAAGTATCAATCCCTTAGGTTAAATCTTAATCATTATAGATTAGTGGTTTCCTGAGCAAGCTAGGTTTTGAAAATCAGTATCAACCTATCTTTATATAAAGTGATGTCGTAATGAGGGCTTTTCCTTTTTGGGTGGGTGGGTTTGTTGGCATATGAACATTGATGTTACTGATAACGGGAAGTAATCTGTACTTTTGTGGAGATAGTTTGTAGGGATCTATTGTGTGTTATGTTAGTATTTTATATTCAAAATACAGTTCATGGTGTCTTTTTCAACATTTACAAGTTTTACATTTTTATTTTTTGGTTTAAACACGATAAGATTATATAGTTTTTCTACCCCAACTCCAAGAAAATTTCCTGATCATAATATGCAACATAAAAATTTCTTGCATGCCTAGAACCATATTAATTCTTCCTGATCAAATATTGTTATCCATTCTTCAGGGAGGAAGAAAATTCTAATATCGCAATAGATCTTAAGAAGCAGCCCAAGCAATTATCGAGACGATCCTATATCAATAGAGTAAAAGAGATCACAAAAAACAGTCGGAAACAAGACACTGACATTGATCGAATCCTTAAAGAAACTAGAGAGCTTCAACTAGAAAGTAACAATATTCAAGAACGCCTCCATAGAACCTATGCAGTTGTGGATGAGCTAGTTCTCAGGTATGCATCACAGAACTCTTGCATCGTTGACTAATGTGGTGGTATTAACGTCTCGCTTTCTCGTCCCTCTTTTACATTTCATTCTCAACCCGAAAAAGAAAATTCGAGGTTTTATCTTGCTGAGAAAACTAGCTGAAGTGATTCATGTCTATGCAGGGAAGCAAAAAAAGATGACACAGGCAAACAAGCACACAAGCTTTTAACTAATATCCATGAGAATTTTGGCGAGATCTCGAACAAAATCCTCTCAACTGATAGACTAAGAAGAGAAACTGCAGAATACGAGAAGAAGATAGCGGCCTCGGCATCCCGAAGCTTAAATTTTAACAAACTACAAGCTGATCTGGATGCTATCAGGAGAGAAAATGGTCACCTGGAGCAACATCTATGCCATAAAAAAACCATTGACAAAGTGTAG

mRNA sequence

ATTTGATATATAATTTTTTGCAATTTGATGAAAAAAAGCCAAAGAAAAATCCATTTTCGGGTGTTTGATTTGTTCAGCAAGAAGTGTTAAGAACGAGAAGGAAAACGGCAAAAGTTCAACCGGAGCAATGGAGGAATCTCAGGAAATTCTTCTCAATTCTCTCCAAAAATCAGGTGTTTCAATCCCAACAGACCTTTCCTCGATTCGGAACTTATCCCCTCCCATATTCTTCTCCATCTGTGCTCAAGTTTTGAATCGAATACATCCCCAAACTAAAACCCCCTTCTCCACTTCTCTTCCGGACTCTGTCGCCGATCAATTCAAGGTATCTTCTGAAATCGCTTCCGCCATTAAGGATCTGGGTTTCATCGGAGATATTAGCTTCCACAAGTTCTTGTATCCATCTGAAGAGGACTTGTACAAGCTGATAAGATTCTTGGTGGAGAGGCTTCCTGAAGCTTCAGATGGAAAATCTAAGGTTTTGGAAGATGGTGATGGTAGGAAAGAAACAAAAGCCGACACTTCCAAGGGCTACTATGTGGAGAACCAATCAAACAAGATCGATACTGACGATTCCGTTTCTACCCATCAGAAGGTTGAGAACAAACTTGCTGATCTCAATATTGTTGCTGAAGAGACAAAATCTCCAGATTCCATCGTTGATAGATTCTCTGATTTCCATCTCGACCGAAAAAGTTCGGGTGAAGCAGCTATGGTCGATAATCTTGTTAACGCTCCCAAGGATCAGTCTGAGGTGTCTGGAAATGAATCAGTTGAACTGCAGGGGAATAATAGACACGAAGTTGGTACTTACATTCAGAAGACCTTTGAAGATCAAGAGATCGGACCATTGGAAGAGGTCACTACAAATGCTTCTGAACTAAAGCATTTTCAAGTGGAGTTGGAAAGGTTGAAGGCAGTAACCAGTATTGTATTTGATGACAACCATTCGATTGAATTTCATCTTCGGCAGCTGGAGGAGCAAATAAATTCTAGAAAACTGGATCTTCTTGAAATGAAGTCACAGTGGGATGCAGAGAGGGAGCTTTTAGAAATTAAAAGGCGAAGTCTACAAGAATCACTATGTGCAAGTAATCCAAAGGCTCAAGAAAAGCTGCAAAAATTGAGGGAGTTTGAATCGGAAAAGAAGCTCATTGAGTTGGAAATCAGGAGGCGGGAGGAAGAAAATTCTAATATCGCAATAGATCTTAAGAAGCAGCCCAAGCAATTATCGAGACGATCCTATATCAATAGAGTAAAAGAGATCACAAAAAACAGTCGGAAACAAGACACTGACATTGATCGAATCCTTAAAGAAACTAGAGAGCTTCAACTAGAAAGTAACAATATTCAAGAACGCCTCCATAGAACCTATGCAGTTGTGGATGAGCTAGTTCTCAGGGAAGCAAAAAAAGATGACACAGGCAAACAAGCACACAAGCTTTTAACTAATATCCATGAGAATTTTGGCGAGATCTCGAACAAAATCCTCTCAACTGATAGACTAAGAAGAGAAACTGCAGAATACGAGAAGAAGATAGCGGCCTCGGCATCCCGAAGCTTAAATTTTAACAAACTACAAGCTGATCTGGATGCTATCAGGAGAGAAAATGGTCACCTGGAGCAACATCTATGCCATAAAAAAACCATTGACAAAGTGTAG

Coding sequence (CDS)

ATGGAGGAATCTCAGGAAATTCTTCTCAATTCTCTCCAAAAATCAGGTGTTTCAATCCCAACAGACCTTTCCTCGATTCGGAACTTATCCCCTCCCATATTCTTCTCCATCTGTGCTCAAGTTTTGAATCGAATACATCCCCAAACTAAAACCCCCTTCTCCACTTCTCTTCCGGACTCTGTCGCCGATCAATTCAAGGTATCTTCTGAAATCGCTTCCGCCATTAAGGATCTGGGTTTCATCGGAGATATTAGCTTCCACAAGTTCTTGTATCCATCTGAAGAGGACTTGTACAAGCTGATAAGATTCTTGGTGGAGAGGCTTCCTGAAGCTTCAGATGGAAAATCTAAGGTTTTGGAAGATGGTGATGGTAGGAAAGAAACAAAAGCCGACACTTCCAAGGGCTACTATGTGGAGAACCAATCAAACAAGATCGATACTGACGATTCCGTTTCTACCCATCAGAAGGTTGAGAACAAACTTGCTGATCTCAATATTGTTGCTGAAGAGACAAAATCTCCAGATTCCATCGTTGATAGATTCTCTGATTTCCATCTCGACCGAAAAAGTTCGGGTGAAGCAGCTATGGTCGATAATCTTGTTAACGCTCCCAAGGATCAGTCTGAGGTGTCTGGAAATGAATCAGTTGAACTGCAGGGGAATAATAGACACGAAGTTGGTACTTACATTCAGAAGACCTTTGAAGATCAAGAGATCGGACCATTGGAAGAGGTCACTACAAATGCTTCTGAACTAAAGCATTTTCAAGTGGAGTTGGAAAGGTTGAAGGCAGTAACCAGTATTGTATTTGATGACAACCATTCGATTGAATTTCATCTTCGGCAGCTGGAGGAGCAAATAAATTCTAGAAAACTGGATCTTCTTGAAATGAAGTCACAGTGGGATGCAGAGAGGGAGCTTTTAGAAATTAAAAGGCGAAGTCTACAAGAATCACTATGTGCAAGTAATCCAAAGGCTCAAGAAAAGCTGCAAAAATTGAGGGAGTTTGAATCGGAAAAGAAGCTCATTGAGTTGGAAATCAGGAGGCGGGAGGAAGAAAATTCTAATATCGCAATAGATCTTAAGAAGCAGCCCAAGCAATTATCGAGACGATCCTATATCAATAGAGTAAAAGAGATCACAAAAAACAGTCGGAAACAAGACACTGACATTGATCGAATCCTTAAAGAAACTAGAGAGCTTCAACTAGAAAGTAACAATATTCAAGAACGCCTCCATAGAACCTATGCAGTTGTGGATGAGCTAGTTCTCAGGGAAGCAAAAAAAGATGACACAGGCAAACAAGCACACAAGCTTTTAACTAATATCCATGAGAATTTTGGCGAGATCTCGAACAAAATCCTCTCAACTGATAGACTAAGAAGAGAAACTGCAGAATACGAGAAGAAGATAGCGGCCTCGGCATCCCGAAGCTTAAATTTTAACAAACTACAAGCTGATCTGGATGCTATCAGGAGAGAAAATGGTCACCTGGAGCAACATCTATGCCATAAAAAAACCATTGACAAAGTGTAG

Protein sequence

MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDSVADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLEDGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDRFSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIGPLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQWDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVDELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLNFNKLQADLDAIRRENGHLEQHLCHKKTIDKV*
Homology
BLAST of CSPI03G04130 vs. ExPASy Swiss-Prot
Match: Q9JIG7 (Coiled-coil domain-containing protein 22 OS=Mus musculus OX=10090 GN=Ccdc22 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.3e-27
Identity = 149/624 (23.88%), Postives = 267/624 (42.79%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEE+  IL++SL+++G ++P ++ ++R  +  +      + L  I+P   +  S  LP +
Sbjct: 1   MEEADRILIHSLRQAGTAVPPEVQTLRAFTTELVVEAVVRCLRVINPDVGSGLSHLLPPA 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLP-EASD------ 120
           ++ +F+++  +A A  DLG+  ++ +  FLYPSE DL  L+ FL ERLP +AS+      
Sbjct: 61  MSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPSDASEDADQPA 120

Query: 121 GKSKVLEDGDGR---------------KETKADTSKGYYVEN--QSNKIDTDDSVSTHQK 180
           G S +     G                +  K    +G  ++    S+++   +  S+ + 
Sbjct: 121 GDSAIFLRAIGSQIRDQLALPWVPPLLRTPKVQRLQGSALQQPFHSSRLVLPELNSSGEL 180

Query: 181 VENKLADLNIVAEETKSPD------SIVDRFSD---FHLDRKSSGEAAMVDNLVNAPKDQ 240
            E + + L ++   T+ P       S+++  +     H++R   G+   V      P  +
Sbjct: 181 WEFQASPL-LLPAPTQVPQLQGRAASLLEHHASQLCQHVNRDCPGDEDRVRWASRVPSQE 240

Query: 241 SEVSGNESVELQ--------------GNNRHEVGTYIQ---------------------- 300
              +  + +  Q                   ++G  +Q                      
Sbjct: 241 DSRAPQQRLHKQLIEHLRQSWGPLGAPTQVRDLGEMLQTWGARAMTGVPKGSRFTHSEKF 300

Query: 301 ---------------------------KTFEDQEIGPLEE----VTTNASELKH------ 360
                                      +  ++QE+  L E    V  N  E++       
Sbjct: 301 TFHLEPQVQAAQVADVPATSQRLEQDTRAAQEQELESLREQLASVNHNIEEVEADMKTLG 360

Query: 361 ---FQVELE---------------RLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLE 420
               QVE E               RLK+ T  +  D  +   +L +L+  + S    L+ 
Sbjct: 361 INLVQVETECRQSELSVAEQEQALRLKSRTVELLPDGAA---NLAKLQLVVESSAQRLIH 420

Query: 421 MKSQWDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSN 480
           + SQW+  R  L  + R L+        ++  +L +++E     +    E RR+EE    
Sbjct: 421 LASQWEKHRVPLLAEYRHLRRLQDCRELESSRRLAEIQELHHSVRAAAEEARRKEEVYKQ 480

Query: 481 IAIDLKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTY 499
           +  +L+  PK +SR +Y  R+ EI  N RKQ  +I +IL +T+ELQ E N++  +L RT+
Sbjct: 481 LVSELETLPKDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTF 540

BLAST of CSPI03G04130 vs. ExPASy Swiss-Prot
Match: P86182 (Coiled-coil domain-containing protein 22 OS=Rattus norvegicus OX=10116 GN=Ccdc22 PE=1 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 3.7e-27
Identity = 154/635 (24.25%), Postives = 264/635 (41.57%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEE+  IL++SL+++G ++P ++ ++R  +  +      + L  I+P   +  S  LP +
Sbjct: 1   MEEADRILIHSLRQAGTAVPPEVQTLRAFTTELVVEAVVRCLRVINPDVGSGLSHLLPPA 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLP-EASD------ 120
           ++ +F+++  +A A  DLG+  ++ +  FLYPSE DL  L+ FL ERLP +AS+      
Sbjct: 61  MSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPA 120

Query: 121 GKSKVLEDGDGR---------------KETKADTSKGYYVEN------------------ 180
           G S +     G                +  K    +G  ++                   
Sbjct: 121 GDSAIFLRAIGSQIRDQLALPWVPPLLRTPKVQRLQGSALQQAFHSSRLVLPELNCRGEP 180

Query: 181 ---------------------------------------------------QSNKIDTDD 240
                                                               S     +D
Sbjct: 181 REFQASPLLLPAPSQVPQLLGRAASLLEHHAIQLCQHVNRDCPGDEDRVRWASRLPSQED 240

Query: 241 SVSTHQKVENKLAD-LNIVAEETKSPDSIVD--------------------RFS-----D 300
             +  Q++  +L + L        +P  + D                    RF+      
Sbjct: 241 PRAPQQRLHKQLIEHLRQSWGPLGAPTQVRDLGEMLQAWGAKAMTGVPKGSRFTHSEKFT 300

Query: 301 FHLD-RKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFED-QEIG- 360
           FHL+ +  + + A +      P+  +  +  E +E        V   I++   + + +G 
Sbjct: 301 FHLEPQVQAAQVADIPAASQRPEQDTRAAQEEELESLREQLASVNHNIEEVEANMKSLGM 360

Query: 361 PLEEVTTNASELKHFQVELE---RLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEM 420
            L +V T   + +    E E   RLK+ T  +  D  +   +L +L+  + S    L+ +
Sbjct: 361 NLVQVETECRQSELSVAEQEQALRLKSRTVELLPDGAA---NLTKLQLVVESSAQRLIHL 420

Query: 421 KSQWDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIEL------------ 480
            SQW+  R  L  + R L+            KLQ  RE ES ++L+E+            
Sbjct: 421 ASQWEKHRVPLLAEYRHLR------------KLQDCRELESSRRLVEIQELHQSVRAAAE 480

Query: 481 EIRRREEENSNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLES 499
           E RR+EE    +  +L+  PK +SR +Y  R+ EI  N RKQ  +I +IL +T+ELQ E 
Sbjct: 481 EARRKEEVYKQLVSELETLPKDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEI 540

BLAST of CSPI03G04130 vs. ExPASy Swiss-Prot
Match: Q28G12 (Coiled-coil domain-containing protein 22 OS=Xenopus tropicalis OX=8364 GN=ccdc22 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 6.3e-27
Identity = 143/633 (22.59%), Postives = 261/633 (41.23%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEE   IL++SL+  G  +P D+ SIR  +  +      + L  I+P      S  LP  
Sbjct: 1   MEEVDRILIHSLRSCGTEVPEDVQSIRQFNTELIVEAVVRCLRVINPSLGATLSHVLPPG 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASD------- 120
           ++ +F++ + +A A +DLG+ G++ +  FLY SE D+  L+ FL E+LP  S        
Sbjct: 61  MSARFRIGTSLAQACQDLGYPGEVGYQTFLYSSEPDIRALLIFLAEKLPRDSPEDAHQPA 120

Query: 121 GKSKVLE--------------------------------------------DGDGRKETK 180
           GKS +L+                                              D   ++ 
Sbjct: 121 GKSALLQREIAATIKRQLSLPWLPSSCRICALRRSQNSCRLHRFHAQPLSLATDPTLKSI 180

Query: 181 ADTSKGY---YVENQSNKIDTDDSVSTHQKVENKLADLNIVAE-----------ETKSPD 240
            D  K Y   Y+ + ++++    SV+    +E   ++L+   E              SP+
Sbjct: 181 PDERKEYWQCYLPSVTSQLPHLPSVAA-SLLERNTSELSAKQEWDAEWKSQGLASRLSPE 240

Query: 241 SIVDR-------------------FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESV 300
               R                    ++ HL   SS +   +    N      +  G+   
Sbjct: 241 DYRSRKRQRLQKRIQEQLRQCAQLLAENHLPSSSSQDLTDMLKAFNLDGGSDQKKGSRFT 300

Query: 301 ELQ----GNNRHEVGTYIQKTFEDQEIGPLEEVTTNASELKHFQVELERLKAVTSIVFDD 360
             Q      + H +   +Q+  E       ++      EL   Q +++ ++     + + 
Sbjct: 301 RTQRFTYQQDPHTLKEQMQRAAEILPKKDAQDTDAEQQELSSLQEQIDSIEQEIRGLSES 360

Query: 361 NHSIEFHLRQLEEQINSRKLDLLEMKSQWDAERELLEIKRRS-------------LQESL 420
           N  ++  + Q+E ++N       EM+   + + +++ +K+R+             LQ  +
Sbjct: 361 NKRLQLTVSQVEGEVN-------EMRQSCEEKEKIVRVKKRAVELLPDADNNLVKLQALV 420

Query: 421 CASN--------------PKAQEKLQKLREFESE------------KKLIEL------EI 480
            AS+               +  E+ ++L+  + E            K L E       E 
Sbjct: 421 DASSHRMANLVGQWESHQVRLSEEYRELKRVQQEQEDESSRWMKDAKDLYEKIRGAADEA 480

Query: 481 RRREEENSNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNN 499
           +R+EE    +  + +  PK++SR +Y  R+ EI  N +KQ  +I +IL +T+ELQ E NN
Sbjct: 481 KRKEELYKQLLSEYESLPKEVSRAAYTQRILEIVSNIKKQKEEITKILSDTKELQKEINN 540

BLAST of CSPI03G04130 vs. ExPASy Swiss-Prot
Match: B0WTU5 (Coiled-coil domain-containing protein 22 homolog OS=Culex quinquefasciatus OX=7176 GN=CPIJ010681 PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.4e-26
Identity = 137/575 (23.83%), Postives = 267/575 (46.43%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           M+E   I+L+SL++ G ++  +++S+   SP +   + ++ +  I      P   +LP  
Sbjct: 1   MDEIDNIILHSLRQIGCTLDDEVTSLDEFSPTLLVQVVSKCITLIDSSLDLP--RTLPPG 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKS---- 120
           +A +F  ++ +A A + +G+  DI +  FLY +  ++ +++ FL+ERLP+ S  K+    
Sbjct: 61  MAQRFTATASLAEACRTIGYRRDIGYQTFLYSNVAEVRRVLMFLIERLPKESADKAAGTG 120

Query: 121 ---KVLEDGDGR-----------------KETKADTSKGY-----YVENQSN--KIDTDD 180
               V  + + R                   T A  S        +V  + N   +   D
Sbjct: 121 QPLDVTTELESRICASLQSQLQAPWMPEFCRTAAGPSSSQIAFRRFVPRKLNIPFVTQGD 180

Query: 181 SVSTHQKVENKLADLNIVAEETKSPDSI-------------------------VDRFSDF 240
             +  ++  ++ A L+ + E++  P  I                         VDR   F
Sbjct: 181 IAAEVKEFWSRQASLDTLDEDSFIPSVIAANDGALKASGGAIDEVDRIKVCSPVDRLQRF 240

Query: 241 HL--DRKSS----GEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTF---- 300
           +   ++KSS    G+ A+ + L      ++   G   +E       ++   I++      
Sbjct: 241 YTSSEQKSSVVPVGKVAVEEKLDALAIVEAARPGKTPLESLQEEIDQIRVEIERAVGEGT 300

Query: 301 ----EDQEIGPLEEVTTNA-SELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINS 360
               E  E+  L EV   A  +LK    + +++K  T I+ +D    E ++R+L+  I +
Sbjct: 301 GLEGERMEVVELCEVQRAAVGKLK----DEKKIKERTHILLEDP---EVNVRKLQGIIGA 360

Query: 361 RKLDLLEMKSQWDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRR 420
               + +++ QWD  R  LE +    +        ++Q+ + ++     + + + ++++ 
Sbjct: 361 GGERMKKLQDQWDVHRIPLEQELEGYRVKHSDKLSQSQQIVDQIEATRHKSEEVMMDLQT 420

Query: 421 REEENSNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQ 480
           +   ++ +  +L+K  + +SR +Y +R+ EI  N RKQ TDID+IL++TR LQ E N I 
Sbjct: 421 KSAMHARLQKELEKLNRTVSRTAYTSRILEIVGNIRKQKTDIDKILQDTRTLQKEINTIT 480

Query: 481 ERLHRTYAVVDELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEK 503
            +L R + V D+L+ R AKKD+  K+A+KLL  +H +  E+   +  T  ++RE  + E 
Sbjct: 481 GQLDRQFTVTDDLIFRNAKKDEHAKKAYKLLVTLHSDCAELMALVQETGAVKREIRDLED 540

BLAST of CSPI03G04130 vs. ExPASy Swiss-Prot
Match: O60826 (Coiled-coil domain-containing protein 22 OS=Homo sapiens OX=9606 GN=CCDC22 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 4.1e-26
Identity = 147/620 (23.71%), Postives = 265/620 (42.74%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEE+  IL++SL+++G ++P D+ ++R  +  +      + L  I+P   +  S  LP +
Sbjct: 1   MEEADRILIHSLRQAGTAVPPDVQTLRAFTTELVVEAVVRCLRVINPAVGSGLSPLLPLA 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLP-EASD------ 120
           ++ +F+++  +A A  DLG+  ++ +  FLYPSE DL  L+ FL ERLP +AS+      
Sbjct: 61  MSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPA 120

Query: 121 GKSKVLEDGDG------------------------------------RKETKADTSKGYY 180
           G S +L    G                                    R      +S+G  
Sbjct: 121 GDSAILLRAIGSQIRDQLALPWVPPHLRTPKLQHLQGSALQKPFHASRLVVPELSSRGEP 180

Query: 181 VENQSNKIDTDDSVSTHQKV-------------------ENKLADLNIVAEETKSPDSIV 240
            E Q++ +         Q V                    ++  D + V   ++ P    
Sbjct: 181 REFQASPLLLPVPTQVPQPVGRVASLLEHHALQLCQQTGRDRPGDEDWVHRTSRLPPQED 240

Query: 241 DRFSDFHLDRKSSGEAAMVDNLVNAP---KDQSEV-----SGNESVELQGN---NRHEVG 300
            R     L ++ +        L+ AP   +D  E+     +G ++   +G+   +  +  
Sbjct: 241 TRAQRQRLQKQLTEHLRQSWGLLGAPIQARDLGELLQAWGAGAKTGAPKGSRFTHSEKFT 300

Query: 301 TYIQKTFEDQEIGPL-------EEVTTNA--SELKHFQVELERLKAVTSIVFDDNHSIEF 360
            +++   +  ++  +       E+VT  A   EL+  + +LE +      V  D  ++  
Sbjct: 301 FHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGV 360

Query: 361 HLRQLEEQ----------------INSRKLDLL----------------------EMKSQ 420
              Q E +                + SR ++LL                       +  Q
Sbjct: 361 SFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQ 420

Query: 421 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 480
           W+  R  L  + R L++       ++  +L +++E     +    E RR+EE    +  +
Sbjct: 421 WEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSE 480

Query: 481 LKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVD 499
           L+  P+ +SR +Y  R+ EI  N RKQ  +I +IL +T+ELQ E N++  +L RT+AV D
Sbjct: 481 LETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTD 540

BLAST of CSPI03G04130 vs. ExPASy TrEMBL
Match: A0A0A0L4U9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G047850 PE=3 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 7.5e-273
Identity = 506/511 (99.02%), Postives = 507/511 (99.22%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGK KVLE
Sbjct: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKFKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           DGDGRKETKADTSK YYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR
Sbjct: 121 DGDGRKETKADTSKCYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240
           FSDFHLDRKSSGEAAMVDNLVNA KDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG
Sbjct: 181 FSDFHLDRKSSGEAAMVDNLVNASKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240

Query: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQ 300
           PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKS+
Sbjct: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSR 300

Query: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360
           WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID
Sbjct: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQLSRRSYINRVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN
Sbjct: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480

Query: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 512
           FNKLQADLDAIRRENGHLEQHLCHKKTIDKV
Sbjct: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 511

BLAST of CSPI03G04130 vs. ExPASy TrEMBL
Match: A0A1S3BKI6 (coiled-coil domain-containing protein 22 OS=Cucumis melo OX=3656 GN=LOC103491028 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.2e-251
Identity = 469/511 (91.78%), Postives = 485/511 (94.91%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKS VSIP DLSSIRNLSPPIFFSICAQVLN+IHPQTKTPFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSSVSIPPDLSSIRNLSPPIFFSICAQVLNQIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFKV S IASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE
Sbjct: 61  VADQFKVCSGIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           DGDGRKETKA+TS+ YYVENQ NK DTDDSVSTHQKV++KLAD NI+AEET+SPDSI+DR
Sbjct: 121 DGDGRKETKANTSRCYYVENQPNKTDTDDSVSTHQKVQDKLADPNIIAEETRSPDSIIDR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240
           FSDF L RKS GEA MVDNLVNAPKD SEV GNESV LQGNNRHEVGTYIQKTFEDQ IG
Sbjct: 181 FSDFCLIRKSLGEAPMVDNLVNAPKDHSEVCGNESVGLQGNNRHEVGTYIQKTFEDQAIG 240

Query: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQ 300
           PLEEVTTNASELKHFQVELERL+AVT IVFDDNHSIEFHL+QLEEQINS+KLDLLEMKSQ
Sbjct: 241 PLEEVTTNASELKHFQVELERLEAVTRIVFDDNHSIEFHLQQLEEQINSKKLDLLEMKSQ 300

Query: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360
           WDAERELLEIKRRSLQESLCA NP AQEKL+KLREFESEKKLIELEIRRREEENSNI ID
Sbjct: 301 WDAERELLEIKRRSLQESLCARNPMAQEKLKKLREFESEKKLIELEIRRREEENSNITID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQ SRRSYINRVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQSSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKD  GKQAHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL+
Sbjct: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLD 480

Query: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 512
           FNKLQADLDAIRREN HLEQHLCHKK +D+V
Sbjct: 481 FNKLQADLDAIRRENSHLEQHLCHKKPVDEV 511

BLAST of CSPI03G04130 vs. ExPASy TrEMBL
Match: A0A5A7VC01 (Coiled-coil domain-containing protein 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00180 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.8e-250
Identity = 469/511 (91.78%), Postives = 485/511 (94.91%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKS VSIP DLSSIRNLSPPIFFSICAQVLN+IHPQTKTPFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSSVSIPPDLSSIRNLSPPIFFSICAQVLNQIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFKV SEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE
Sbjct: 61  VADQFKVCSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           DGDGRKE KA+TS+ YYVENQ NK DTDDSVSTHQKV++KLAD NI+AEET+SPDSI+DR
Sbjct: 121 DGDGRKE-KANTSRCYYVENQPNKTDTDDSVSTHQKVQDKLADPNIIAEETRSPDSIIDR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240
           FSDF L RKS GEA MVDNLVNAPKD SEV GNESV LQGNNRHEVGTYIQKTFEDQ IG
Sbjct: 181 FSDFCLIRKSLGEAPMVDNLVNAPKDHSEVCGNESVGLQGNNRHEVGTYIQKTFEDQAIG 240

Query: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQ 300
           PLEEVTTNASELKHFQVELERL+AVT IVFDDNHSIEFHL+QLEEQINS+KLDLLEMKSQ
Sbjct: 241 PLEEVTTNASELKHFQVELERLEAVTRIVFDDNHSIEFHLQQLEEQINSKKLDLLEMKSQ 300

Query: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360
           WDAERELLEIKRRSLQESLCA NP AQEKL+KLREFESEKKLIELEIRRREEENSNI ID
Sbjct: 301 WDAERELLEIKRRSLQESLCARNPMAQEKLKKLREFESEKKLIELEIRRREEENSNITID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQ SRRSYINRVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQSSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKD  GKQAHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL+
Sbjct: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLD 480

Query: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 512
           FNKLQADLDAIRREN HLEQHLCHKK +D+V
Sbjct: 481 FNKLQADLDAIRRENSHLEQHLCHKKPVDEV 510

BLAST of CSPI03G04130 vs. ExPASy TrEMBL
Match: A0A6J1EW97 (coiled-coil domain-containing protein 22 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438763 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.8e-219
Identity = 420/517 (81.24%), Postives = 459/517 (88.78%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKSGVS+P D+SSIRNL+PP+ FSICAQVLNRIH QT+ P++TSLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSVPPDVSSIRNLTPPLLFSICAQVLNRIHGQTQIPYATSLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFK+ +EIASAIKDLG+IGD+SFHKFLYPSEEDLYKLIRFLVERLPE SDGK+KVLE
Sbjct: 61  VADQFKICTEIASAIKDLGYIGDMSFHKFLYPSEEDLYKLIRFLVERLPEVSDGKTKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           D DGRKETK DT K +YV  QS+ IDTDDSV THQKVENKL+DLNI+AEETKS DSIV R
Sbjct: 121 DVDGRKETKTDTLKCHYVSGQSDDIDTDDSVFTHQKVENKLSDLNIIAEETKSSDSIVGR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNN------RHEVGTYIQKTF 240
           FSD       SGEAAMVDNLVNAPKDQSEVSGNESV +QGNN      RHEV +YIQK  
Sbjct: 181 FSD-------SGEAAMVDNLVNAPKDQSEVSGNESVAVQGNNGQVSISRHEVSSYIQKND 240

Query: 241 EDQEIGPLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDL 300
           EDQEI PLEEV+T ASEL+H  VELERLKA T IVFDDN  IE HL++LEEQI+SRKL+L
Sbjct: 241 EDQEIRPLEEVSTKASELEHLHVELERLKAATGIVFDDNRLIEVHLQELEEQISSRKLNL 300

Query: 301 LEMKSQWDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEEN 360
           L  KS W AE+ELLE KRR+LQESLCASNPKAQEKLQKLREFE EK+LIE EI RREEEN
Sbjct: 301 LAAKSGWAAEKELLENKRRNLQESLCASNPKAQEKLQKLREFELEKQLIESEISRREEEN 360

Query: 361 SNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHR 420
            N++ +LKKQPKQ SRRSYI+RVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHR
Sbjct: 361 YNLSTELKKQPKQASRRSYIDRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHR 420

Query: 421 TYAVVDELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAAS 480
           TYA+VDELVLREAKK+  GK+AHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAAS
Sbjct: 421 TYAIVDELVLREAKKEGIGKKAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAAS 480

Query: 481 ASRSLNFNKLQADLDAIRRENGHLEQHLCHKKTIDKV 512
           ASRSL+FNKLQADLDAIRREN  LEQ+LCHKK ID++
Sbjct: 481 ASRSLDFNKLQADLDAIRRENSCLEQNLCHKKPIDQM 510

BLAST of CSPI03G04130 vs. ExPASy TrEMBL
Match: A0A6J1L5Y0 (coiled-coil domain-containing protein 22 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500292 PE=3 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 5.8e-217
Identity = 417/517 (80.66%), Postives = 456/517 (88.20%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKSGVS+P D+SSIR L+PP+ FSICAQVLNRIH QT+ P++TSLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSVPPDVSSIRKLTPPLLFSICAQVLNRIHGQTQIPYATSLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFK+ +EIASAIKDLG+IGD+SFHKFLYPSEEDLYKLIRFLVERLPE SDGKSKVLE
Sbjct: 61  VADQFKICTEIASAIKDLGYIGDMSFHKFLYPSEEDLYKLIRFLVERLPEVSDGKSKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           DGDGRKETKADT K +YV  QS+ IDT DSV THQKVENKL+DLNI+AEETKS DSIV R
Sbjct: 121 DGDGRKETKADTLKCHYVSGQSDAIDTADSVFTHQKVENKLSDLNIIAEETKSSDSIVGR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNN------RHEVGTYIQKTF 240
            SD       SGE AMVDNLVNAPK QSEVSGNESV +QGNN      RHEV +YIQK  
Sbjct: 181 LSD-------SGETAMVDNLVNAPKYQSEVSGNESVAVQGNNGQVSISRHEVSSYIQKND 240

Query: 241 EDQEIGPLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDL 300
           EDQEI PLEEV+T ASEL+H  VELERLKA T IVFDDN SIE HL++LEEQI+SRKL+L
Sbjct: 241 EDQEIRPLEEVSTKASELEHLLVELERLKAATGIVFDDNRSIEVHLQELEEQISSRKLNL 300

Query: 301 LEMKSQWDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEEN 360
           L  KS WDAE+ELLE KRR+LQESLCASNPKA EKLQKLREFE EK+LIE EIRRREE N
Sbjct: 301 LAAKSGWDAEKELLENKRRNLQESLCASNPKAPEKLQKLREFELEKQLIESEIRRREEGN 360

Query: 361 SNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHR 420
            N++ +LKKQPKQ SRRSYI+RVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHR
Sbjct: 361 YNLSTELKKQPKQASRRSYIDRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHR 420

Query: 421 TYAVVDELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAAS 480
           TYA+VDELVLREAKK+  GK+AHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAAS
Sbjct: 421 TYAIVDELVLREAKKEGIGKKAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAAS 480

Query: 481 ASRSLNFNKLQADLDAIRRENGHLEQHLCHKKTIDKV 512
           ASRSL+FNKLQADLDAIR+EN  LEQ+LCHKK ID++
Sbjct: 481 ASRSLDFNKLQADLDAIRKENSCLEQNLCHKKPIDQM 510

BLAST of CSPI03G04130 vs. NCBI nr
Match: XP_004148284.1 (coiled-coil domain-containing protein 22 isoform X1 [Cucumis sativus])

HSP 1 Score: 949.1 bits (2452), Expect = 1.5e-272
Identity = 506/511 (99.02%), Postives = 507/511 (99.22%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGK KVLE
Sbjct: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKFKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           DGDGRKETKADTSK YYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR
Sbjct: 121 DGDGRKETKADTSKCYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240
           FSDFHLDRKSSGEAAMVDNLVNA KDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG
Sbjct: 181 FSDFHLDRKSSGEAAMVDNLVNASKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240

Query: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQ 300
           PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKS+
Sbjct: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSR 300

Query: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360
           WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID
Sbjct: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQLSRRSYINRVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN
Sbjct: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480

Query: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 512
           FNKLQADLDAIRRENGHLEQHLCHKKTIDKV
Sbjct: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 511

BLAST of CSPI03G04130 vs. NCBI nr
Match: XP_008449035.1 (PREDICTED: coiled-coil domain-containing protein 22 [Cucumis melo])

HSP 1 Score: 878.6 bits (2269), Expect = 2.6e-251
Identity = 469/511 (91.78%), Postives = 485/511 (94.91%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKS VSIP DLSSIRNLSPPIFFSICAQVLN+IHPQTKTPFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSSVSIPPDLSSIRNLSPPIFFSICAQVLNQIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFKV S IASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE
Sbjct: 61  VADQFKVCSGIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           DGDGRKETKA+TS+ YYVENQ NK DTDDSVSTHQKV++KLAD NI+AEET+SPDSI+DR
Sbjct: 121 DGDGRKETKANTSRCYYVENQPNKTDTDDSVSTHQKVQDKLADPNIIAEETRSPDSIIDR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240
           FSDF L RKS GEA MVDNLVNAPKD SEV GNESV LQGNNRHEVGTYIQKTFEDQ IG
Sbjct: 181 FSDFCLIRKSLGEAPMVDNLVNAPKDHSEVCGNESVGLQGNNRHEVGTYIQKTFEDQAIG 240

Query: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQ 300
           PLEEVTTNASELKHFQVELERL+AVT IVFDDNHSIEFHL+QLEEQINS+KLDLLEMKSQ
Sbjct: 241 PLEEVTTNASELKHFQVELERLEAVTRIVFDDNHSIEFHLQQLEEQINSKKLDLLEMKSQ 300

Query: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360
           WDAERELLEIKRRSLQESLCA NP AQEKL+KLREFESEKKLIELEIRRREEENSNI ID
Sbjct: 301 WDAERELLEIKRRSLQESLCARNPMAQEKLKKLREFESEKKLIELEIRRREEENSNITID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQ SRRSYINRVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQSSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKD  GKQAHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL+
Sbjct: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLD 480

Query: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 512
           FNKLQADLDAIRREN HLEQHLCHKK +D+V
Sbjct: 481 FNKLQADLDAIRRENSHLEQHLCHKKPVDEV 511

BLAST of CSPI03G04130 vs. NCBI nr
Match: KAA0065733.1 (coiled-coil domain-containing protein 22 [Cucumis melo var. makuwa] >TYK13759.1 coiled-coil domain-containing protein 22 [Cucumis melo var. makuwa])

HSP 1 Score: 874.8 bits (2259), Expect = 3.7e-250
Identity = 469/511 (91.78%), Postives = 485/511 (94.91%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKS VSIP DLSSIRNLSPPIFFSICAQVLN+IHPQTKTPFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSSVSIPPDLSSIRNLSPPIFFSICAQVLNQIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFKV SEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE
Sbjct: 61  VADQFKVCSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           DGDGRKE KA+TS+ YYVENQ NK DTDDSVSTHQKV++KLAD NI+AEET+SPDSI+DR
Sbjct: 121 DGDGRKE-KANTSRCYYVENQPNKTDTDDSVSTHQKVQDKLADPNIIAEETRSPDSIIDR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240
           FSDF L RKS GEA MVDNLVNAPKD SEV GNESV LQGNNRHEVGTYIQKTFEDQ IG
Sbjct: 181 FSDFCLIRKSLGEAPMVDNLVNAPKDHSEVCGNESVGLQGNNRHEVGTYIQKTFEDQAIG 240

Query: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQ 300
           PLEEVTTNASELKHFQVELERL+AVT IVFDDNHSIEFHL+QLEEQINS+KLDLLEMKSQ
Sbjct: 241 PLEEVTTNASELKHFQVELERLEAVTRIVFDDNHSIEFHLQQLEEQINSKKLDLLEMKSQ 300

Query: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360
           WDAERELLEIKRRSLQESLCA NP AQEKL+KLREFESEKKLIELEIRRREEENSNI ID
Sbjct: 301 WDAERELLEIKRRSLQESLCARNPMAQEKLKKLREFESEKKLIELEIRRREEENSNITID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQ SRRSYINRVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQSSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKD  GKQAHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL+
Sbjct: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLD 480

Query: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 512
           FNKLQADLDAIRREN HLEQHLCHKK +D+V
Sbjct: 481 FNKLQADLDAIRRENSHLEQHLCHKKPVDEV 510

BLAST of CSPI03G04130 vs. NCBI nr
Match: XP_038903178.1 (coiled-coil domain-containing protein 22 isoform X2 [Benincasa hispida])

HSP 1 Score: 821.6 bits (2121), Expect = 3.7e-234
Identity = 441/507 (86.98%), Postives = 469/507 (92.50%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKSGVSIP D+SSIRNL+PP+ FSICAQVLNRIH QTK PFS SLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSIPPDISSIRNLTPPLLFSICAQVLNRIHLQTKIPFSISLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFK+ SEIASAIKDLGFIGD+SFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE
Sbjct: 61  VADQFKICSEIASAIKDLGFIGDMSFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           DGDGRKETKADTSK YYVE+ S+K++TDDSV THQKVENK ADLNI++EETKS +SIV R
Sbjct: 121 DGDGRKETKADTSKCYYVEDPSDKVNTDDSVFTHQKVENKRADLNIISEETKSSNSIVGR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240
           FSD  L ++S GEAAM DNLVNAPKDQSEVSGNESV LQGNNRHEVG+Y +KT EDQEI 
Sbjct: 181 FSDLCLVQQSLGEAAMADNLVNAPKDQSEVSGNESVALQGNNRHEVGSYTEKTCEDQEIR 240

Query: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQ 300
           PLEEVT+ ASEL+H QVELERLKAVT IVFD+N SIEFHL+QLEEQINSR L+L  MKSQ
Sbjct: 241 PLEEVTSKASELEHLQVELERLKAVTRIVFDENQSIEFHLQQLEEQINSRNLNLRAMKSQ 300

Query: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360
           WDAERELLE KR+SLQ+SLCASNPKAQEKLQKLREFESEKKLIE EIRRREEENSN+A D
Sbjct: 301 WDAERELLENKRQSLQDSLCASNPKAQEKLQKLREFESEKKLIESEIRRREEENSNLATD 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVD 420
           +KKQPKQL RRSYINRVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 IKKQPKQLPRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELV REAKKD  GKQAHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN
Sbjct: 421 ELVFREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480

Query: 481 FNKLQADLDAIRRENGHLEQHLCHKKT 508
           F KLQADLDAIRREN +LEQHLC+KKT
Sbjct: 481 FTKLQADLDAIRRENSYLEQHLCYKKT 507

BLAST of CSPI03G04130 vs. NCBI nr
Match: XP_038903177.1 (coiled-coil domain-containing protein 22 isoform X1 [Benincasa hispida])

HSP 1 Score: 817.0 bits (2109), Expect = 9.1e-233
Identity = 441/508 (86.81%), Postives = 469/508 (92.32%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60
           MEESQEILLNSLQKSGVSIP D+SSIRNL+PP+ FSICAQVLNRIH QTK PFS SLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSIPPDISSIRNLTPPLLFSICAQVLNRIHLQTKIPFSISLPDS 60

Query: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120
           VADQFK+ SEIASAIKDLGFIGD+SFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE
Sbjct: 61  VADQFKICSEIASAIKDLGFIGDMSFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKETKADTSKGYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180
           DGDGRKETKADTSK YYVE+ S+K++TDDSV THQKVENK ADLNI++EETKS +SIV R
Sbjct: 121 DGDGRKETKADTSKCYYVEDPSDKVNTDDSVFTHQKVENKRADLNIISEETKSSNSIVGR 180

Query: 181 FSDFHLDRKSSGEAAMVDNLVNAPKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240
           FSD  L ++S GEAAM DNLVNAPKDQSEVSGNESV LQGNNRHEVG+Y +KT EDQEI 
Sbjct: 181 FSDLCLVQQSLGEAAMADNLVNAPKDQSEVSGNESVALQGNNRHEVGSYTEKTCEDQEIR 240

Query: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQ 300
           PLEEVT+ ASEL+H QVELERLKAVT IVFD+N SIEFHL+QLEEQINSR L+L  MKSQ
Sbjct: 241 PLEEVTSKASELEHLQVELERLKAVTRIVFDENQSIEFHLQQLEEQINSRNLNLRAMKSQ 300

Query: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360
           WDAERELLE KR+SLQ+SLCASNPKAQEKLQKLREFESEKKLIE EIRRREEENSN+A D
Sbjct: 301 WDAERELLENKRQSLQDSLCASNPKAQEKLQKLREFESEKKLIESEIRRREEENSNLATD 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVD 420
           +KKQPKQL RRSYINRVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 IKKQPKQLPRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVL-REAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL 480
           ELV  REAKKD  GKQAHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL
Sbjct: 421 ELVFSREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL 480

Query: 481 NFNKLQADLDAIRRENGHLEQHLCHKKT 508
           NF KLQADLDAIRREN +LEQHLC+KKT
Sbjct: 481 NFTKLQADLDAIRRENSYLEQHLCYKKT 508

BLAST of CSPI03G04130 vs. TAIR 10
Match: AT1G55830.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF812 (InterPro:IPR008530). )

HSP 1 Score: 346.7 bits (888), Expect = 3.3e-95
Identity = 222/513 (43.27%), Postives = 321/513 (62.57%), Query Frame = 0

Query: 2   EESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDSV 61
           EES++IL+ +L +SGVSIP D +S+   +P    SICAQ+LN I P     FS  LPDS+
Sbjct: 3   EESRDILMTTLIESGVSIPGDFTSVSEFTPEALVSICAQLLNLIDPSAS--FSDELPDSL 62

Query: 62  ADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLED 121
            ++F++ ++IA ++K+LG+I D+S++KFL+PSE+D Y+L+RFLVERL E S+G+ K L  
Sbjct: 63  PERFRICTDIAHSVKNLGYINDMSYYKFLHPSEDDSYRLVRFLVERLSEISEGR-KTLTA 122

Query: 122 GDGRKETKADTSKGYYVENQSNKIDTDDSVSTH-QKVENKLADLNIVAEETKSPDSIVDR 181
           GD     K +T +    +   N+ D D++   H QKVE  L DL + +E++ S DS+   
Sbjct: 123 GDIASRPKMETFRDISDDMMVNE-DKDETFDMHIQKVEAVLKDLTMTSEKSHSSDSLAKN 182

Query: 182 FS---DFHLDRKSSGEAAMVDNLVNAPKDQSEVSGN----------ESVELQGNNRHEVG 241
            S   DF   +       +  +L  + +D S    N          E+VELQ  N+H+V 
Sbjct: 183 TSANVDFSSQKTDDPVTDVRSDL--SLRDSSRCEENSYEDPFETNYETVELQ--NQHDV- 242

Query: 242 TYIQKTFEDQEIGPLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQI 301
                         LEE+ + +S+L   + ELE L+     + DD      +L QL +Q+
Sbjct: 243 -------------LLEELESGSSQLCSLESELELLQMAAERLLDDKKPGGSYLEQLNQQL 302

Query: 302 NSRKLDLLEMKSQWDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEI 361
             ++ +++++K QWD  R  LE K+  L + L    P+A+EK  KLR+ E + + +  EI
Sbjct: 303 VVKRCNIMDLKKQWDDVRLTLETKKLLLLDQLHVEEPEAKEKFHKLRKTELDLQSLSSEI 362

Query: 362 RRREEENSNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDTDIDRILKETRELQLESNN 421
           ++RE+E  N+  +L++QPK   R+SYI+ +KEITKNSRK DTDI RI  ETRELQLE N+
Sbjct: 363 QKREDERCNLYNELERQPKAAPRKSYIHGIKEITKNSRKLDTDIQRISGETRELQLEKNS 422

Query: 422 IQERLHRTYAVVDELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEY 481
           IQERLHR+YAVVDE+V RE KKD   +Q +KLLT+IH  F +IS KIL TDR RRET +Y
Sbjct: 423 IQERLHRSYAVVDEMVTREVKKDPAVRQVYKLLTSIHSIFEQISEKILMTDRFRRETVDY 482

Query: 482 EKKIAASASRSLNFNKLQADLDAIRRENGHLEQ 501
           EKK+ +  +R ++  KLQADLDAIR+EN  L++
Sbjct: 483 EKKLGSITARGMSLEKLQADLDAIRKENESLKK 493

BLAST of CSPI03G04130 vs. TAIR 10
Match: AT1G55830.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF812 (InterPro:IPR008530); Has 10940 Blast hits to 7289 proteins in 653 species: Archae - 228; Bacteria - 791; Metazoa - 6174; Fungi - 805; Plants - 342; Viruses - 19; Other Eukaryotes - 2581 (source: NCBI BLink). )

HSP 1 Score: 268.9 bits (686), Expect = 8.6e-72
Identity = 182/427 (42.62%), Postives = 259/427 (60.66%), Query Frame = 0

Query: 88  KFLYPSEEDLYKLIRFLVERLPEASDGKSKVLEDGDGRKETKADTSKGYYVENQSNKIDT 147
           +FL+PSE+D Y+L+RFLVERL E S+G+ K L  GD     K +T +    +   N+ D 
Sbjct: 6   QFLHPSEDDSYRLVRFLVERLSEISEGR-KTLTAGDIASRPKMETFRDISDDMMVNE-DK 65

Query: 148 DDSVSTH-QKVENKLADLNIVAEETKSPDSIVDRFS---DFHLDRKSSGEAAMVDNLVNA 207
           D++   H QKVE  L DL + +E++ S DS+    S   DF   +       +  +L  +
Sbjct: 66  DETFDMHIQKVEAVLKDLTMTSEKSHSSDSLAKNTSANVDFSSQKTDDPVTDVRSDL--S 125

Query: 208 PKDQSEVSGN----------ESVELQGNNRHEVGTYIQKTFEDQEIGPLEEVTTNASELK 267
            +D S    N          E+VELQ  N+H+V               LEE+ + +S+L 
Sbjct: 126 LRDSSRCEENSYEDPFETNYETVELQ--NQHDV--------------LLEELESGSSQLC 185

Query: 268 HFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSQWDAERELLEIKRR 327
             + ELE L+     + DD      +L QL +Q+  ++ +++++K QWD  R  LE K+ 
Sbjct: 186 SLESELELLQMAAERLLDDKKPGGSYLEQLNQQLVVKRCNIMDLKKQWDDVRLTLETKKL 245

Query: 328 SLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAIDLKKQPKQLSRRSY 387
            L + L    P+A+EK  KLR+ E + + +  EI++RE+E  N+  +L++QPK   R+SY
Sbjct: 246 LLLDQLHVEEPEAKEKFHKLRKTELDLQSLSSEIQKREDERCNLYNELERQPKAAPRKSY 305

Query: 388 INRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHRTYAVVDELVLREAKKDDTG 447
           I+ +KEITKNSRK DTDI RI  ETRELQLE N+IQERLHR+YAVVDE+V RE KKD   
Sbjct: 306 IHGIKEITKNSRKLDTDIQRISGETRELQLEKNSIQERLHRSYAVVDEMVTREVKKDPAV 365

Query: 448 KQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLNFNKLQADLDAIRR 501
           +Q +KLLT+IH  F +IS KIL TDR RRET +YEKK+ +  +R ++  KLQADLDAIR+
Sbjct: 366 RQVYKLLTSIHSIFEQISEKILMTDRFRRETVDYEKKLGSITARGMSLEKLQADLDAIRK 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9JIG71.3e-2723.88Coiled-coil domain-containing protein 22 OS=Mus musculus OX=10090 GN=Ccdc22 PE=1... [more]
P861823.7e-2724.25Coiled-coil domain-containing protein 22 OS=Rattus norvegicus OX=10116 GN=Ccdc22... [more]
Q28G126.3e-2722.59Coiled-coil domain-containing protein 22 OS=Xenopus tropicalis OX=8364 GN=ccdc22... [more]
B0WTU52.4e-2623.83Coiled-coil domain-containing protein 22 homolog OS=Culex quinquefasciatus OX=71... [more]
O608264.1e-2623.71Coiled-coil domain-containing protein 22 OS=Homo sapiens OX=9606 GN=CCDC22 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0L4U97.5e-27399.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G047850 PE=3 SV=1[more]
A0A1S3BKI61.2e-25191.78coiled-coil domain-containing protein 22 OS=Cucumis melo OX=3656 GN=LOC103491028... [more]
A0A5A7VC011.8e-25091.78Coiled-coil domain-containing protein 22 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A6J1EW972.8e-21981.24coiled-coil domain-containing protein 22 isoform X1 OS=Cucurbita moschata OX=366... [more]
A0A6J1L5Y05.8e-21780.66coiled-coil domain-containing protein 22 isoform X1 OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
XP_004148284.11.5e-27299.02coiled-coil domain-containing protein 22 isoform X1 [Cucumis sativus][more]
XP_008449035.12.6e-25191.78PREDICTED: coiled-coil domain-containing protein 22 [Cucumis melo][more]
KAA0065733.13.7e-25091.78coiled-coil domain-containing protein 22 [Cucumis melo var. makuwa] >TYK13759.1 ... [more]
XP_038903178.13.7e-23486.98coiled-coil domain-containing protein 22 isoform X2 [Benincasa hispida][more]
XP_038903177.19.1e-23386.81coiled-coil domain-containing protein 22 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G55830.23.3e-9543.27unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G55830.18.6e-7242.62unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF812 ... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 198..218
NoneNo IPR availableCOILSCoilCoilcoord: 244..271
NoneNo IPR availableCOILSCoilCoilcoord: 309..329
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..51
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..59
IPR008530Coiled-coil domain-containing protein 22PFAMPF05667DUF812coord: 146..348
e-value: 1.6E-27
score: 96.6
IPR008530Coiled-coil domain-containing protein 22PANTHERPTHR15668JM1 PROTEINcoord: 102..347
coord: 4..37

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G04130.1CSPI03G04130.1mRNA
CSPI03G04130.2CSPI03G04130.2mRNA
CSPI03G04130.3CSPI03G04130.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:2000060 positive regulation of ubiquitin-dependent protein catabolic process
molecular_function GO:0097602 cullin family protein binding