CSPI03G04030 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G04030
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCaffeic acid 3-O-methyltransferase-like
LocationChr3: 3323753 .. 3327122 (+)
RNA-Seq ExpressionCSPI03G04030
SyntenyCSPI03G04030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATATGTGAGAGATCTGACTTAAGAGACAGTCTTCTTAAGCTGTCATCTGGATTCATGGACCTTGATTCTTCTCTGAAATCGATTGCATCAATTTCCTTTAATATCATAGTTTTGTGTTTGACCATGGAGTTCATTTTGTCAGTTTGCCAAGAATTAATGTTTTTTGTTAAAGCCTTGAGGCTCTAAATGAAAGCGAAACCTCGATGACCATCCTCTTTAGCATTATTCCACCACATATCTAGGTTTCTTTTAAAATCAGCTTCTTTTATAAAGTTGGTATTAAGCCTGAAGGGAGAAGGGCCCCATCTGATATCTGTCGATTCAAGGACTATAGAGTGGTGATCATAAATAATTCTGAAAAGCGATCTTGAAAAATTAAAGTTGAAAGCTCGATCTCAATAAGGGTTGAAAAGAAATCTATTGATCCGGGAGAGCACTGGATTAGTTCTGAGATTGGACCAAGTGAACTTGATGTTGGACAGAGGAGGGTCAATTAAGTTATTGAACTGTATGAAATTGTTGGAACTTTTTCATACTTAACTTAGACGAACTGATTGCAGTAGTTTCCTTCTTCCATCTTATGACAATTGAAATCTCCCCTCAAAAGCTAAAGAGAGTTATTTAGAGAATTAAGCTTGTGAAGGTTTTCCCAGAAAAGATCACGATTTCTTCTTCTTGCAGGGTCATAAATAGTAGTTAGCCACCAACTGATGCCTTTTGAAACTGCCTTAGCATAGGAGCGTCTTGGGGTGCTATCTAAGGTACCTTTTGGGGTGAATGAGGGTGTAGTTTTGGCTTCAACTTCAACTTTTGGCGTGATCGTTGAAAGAAAGGAAACTCAATCACTTTTGTCGATGCCCTCTGGGACAAGAATGCATGCCTTTCTTCCGACATAATCAACTCTATACATCTTCGTTGTGTAACCTTTTCTGTTTTTTGTTTTTCTGATCCAAAGACAGTGGTCTTCATTTCTTGTTTCCAGGAAGAACCGTATTATACTTGGAGTAGCAATCAGATTTTTGAGTGTGTTCCTAACCCATTCTAGTTCCGAGGTTACTTCCATAGAGAATGTCTTATGTGCTCCCACTTCAGTTAGCCAGTACAACATAGATTTTGAATATTTGTCTAGGAGAGAGGAGATATTCTTTTCTTTCTATTTTGCATGAACAAGGAAGATTCTTGAAGAACGCCATAATTGAGAGTTGTTGCAGGTCTAAAAGTAGTAACAGGTTGAAGAGAAAGGTGAGAAGAAGAAGTAGTGTGTGTACCTTTTAAAGCGGATGCATGGCGTTGTGTCATGATGAAAGTTCCGAGAGGGGGTATTACTATTGCGACGGATGGGATTCTTGGAGAGAGGAGAGGGAAGATATGGAGCGCATATATGGTTTGGACTAAGTTAAAATTTATGTCTCATGGTTTATAATTAAAATTAATCTCATTAGGTTTGGTAAAAACTTCATAACTAAGTACCTTATTGTATGGACTATTTAAGAGAATTTAACCAACCATAAGAAACTAAGTTATATGGAGTAGTATGAAATCATAGAAACTAAGACTTTGGTAGCATTATTATTATTATTATTAAATTAAGTCTATAGATACTAGTCCTACCTCTAAATTTCTTCATTTGTCGTCTTCTATTTACTTATTGTTTAAAAAATTAAACCATATTTTGACAACGAAAAAAAGTACTTTTAAAATTTTGATTTTTTTTTTTAATTTGGCTAAGAATTTAACTATCTATTTGAGAAAAATGTAAATCATTATAAGAAATGGGGAGGAAATCAACTTAAGTTTTTTTTTAAAAAAAACAAAATGATACTGAAACAAAAACTAAATTCTAAATTTTAAAATCACATTGACAAATTAACTTTATCCAACCATAGAGACCAAAATTTGAAATTTAACGGAATTTAACTTATAATAAATATTTTTAATTTAAAAATATTTGAATTTGATTAACAAATGCTTCTAAATATTTCTATCTTTATCTAATATAATTTTAAAATTCAAATTATGGATACAAATTAACTTAAGGTGACATTTTTTTGGATGTTGGCGGACGTTTTTTTCATGAGTAAAAGTGAACATTCTTATAGTGTTAGATGGTTAGTTTGGATTATAACATAATCTGGAAAAGAAAAAAAAAGCTTTCATTAAAGAGAACAAAATTTGGATTGTGAACCAAACATAACCGAGTTTTTTAAAATCGCAGGAGTGAGTTGAAGAATGCAGTGATTGAAGGAGGAGTTCCTTTTGACCGAGCTCATGGAGGAGTTCATGCATTTGAATACCCTAAGTTAGATCCCAGATTCAACCAAGTATTTAACATTGCAATGATAAATCACACTACAATGTCCATAAAGAAAATTGTGGAATCATACAAAGGTTTTGCAAACATCAAGCAATTAGTTGATGTTGGTGGTGGCCTTGGGGTCACCCTTCAAATCATCACTTCTACCTATCCTTCCATCAAAGGCATCAATTTCGACTTACCTCATGTCATCCGTGATGCCCCTGCTTATAATAGTAAGTAGTTCCAATCATTTAATTTGAAAAAGTTTACAAAGCTGAATTTTGATTATGAATTGGCAGGGGTTCAACATGTGGGAGGAGACATGTTTGAGAGTGTTCCAAATGGAGATGCTATTTTCATGAAGGTTAGTTTTCTTTTCATTTTCATAAACAAAAACATTCATAAACAATAGTTCCTATGCTAGAGACTATTTTTTTTAGGACTTTATCGAATTTTAGAGACTATTCATAAGATTTTATATATAAAAAAGAATCTAACTTTTAACTTTTGAATTTGTAATTTAACCAATAATAATTAACCTATAGTTGAGAGATATAGGCCACTCTGCCCTTTGAAAACTGTCAACATTTAAGATTGTACAATTAGTATTTTATGGACTAAGTCATACTTGAAATGACAAAATTCTTTTACTTTATTGTTATTATAGTGGATATTACATGATTGGAGCGACGATCATTGCACAAAGTTATTGAAAAATTGCTATAATGCAATTCCAGATGATGGAAAGATAATTATAGTAGACAGTGTGATTCCCACGGAGCCTGAAACCACAAATGTAACAAAGGCAACAGCCCAAGCTGATGTGCTTATGATGACTCAAAATCCAGGAGGAAAGGAAAGAACTAGAGATGAATTTAAGAGCCTAGCAACCAAAGCTGGGTTTAAACATGTCATATTCCAATGTTTTGTATCTAATCTTTGGGTTATAGAGTTTCTTAAAAATTAATTTATATATATTGTAATTGAATTTCTATGTTTCTATGTCCTTTTGGTTGCTAAACTTTCTGCACTCCAAAACGTTGAATAAAAAAGTTGTTATTGTTGTTATAA

mRNA sequence

ATGATATGTGAGAGATCTGACTTAAGAGACAGTCTTCTTAAGCTGTCATCTGGATTCATGGACCTTGATTCTTCTCTGAAATCGATTGCATCAATTTCCTTTAATATCATAGTTTTGTGTTTGACCATGGAGTTCATTTTCTTCTTTTATAAAGTTGGTATTAAGCCTGAAGGGAGAAGGGCCCCATCTGATATCTGTCGATTCAAGGACTATAGAGTGAAATCTATTGATCCGGGAGAGCACTGGATTAGTTCTGAGATTGGACCAAGTGAACTTGATGTTGGACAGAGGAGGGAGCGTCTTGGGGTGCTATCTAAGGTACCTTTTGGGGTGAATGAGGGTGTAGTTTTGGCTTCAACTTCAACTTTTGGCGTGATCGTTGAAAGAAAGGAAACTCAATCACTTTTGAAGAACCGTATTATACTTGGAGTAGCAATCAGATTTTTGAGTGTGTTCCTAACCCATTCTAGTTCCGAGGTTACTTCCATAGAGAATGTCTTATGTGCTCCCACTTCAGTTAGCCAGAGTGAGTTGAAGAATGCAGTGATTGAAGGAGGAGTTCCTTTTGACCGAGCTCATGGAGGAGTTCATGCATTTGAATACCCTAAGTTAGATCCCAGATTCAACCAAGTATTTAACATTGCAATGATAAATCACACTACAATGTCCATAAAGAAAATTGTGGAATCATACAAAGGTTTTGCAAACATCAAGCAATTAGTTGATGTTGGTGGTGGCCTTGGGGTCACCCTTCAAATCATCACTTCTACCTATCCTTCCATCAAAGGCATCAATTTCGACTTACCTCATGTCATCCGTGATGCCCCTGCTTATAATAGGGTTCAACATGTGGGAGGAGACATGTTTGAGAGTGTTCCAAATGGAGATGCTATTTTCATGAAGTGGATATTACATGATTGGAGCGACGATCATTGCACAAAGTTATTGAAAAATTGCTATAATGCAATTCCAGATGATGGAAAGATAATTATAGTAGACAGTGTGATTCCCACGGAGCCTGAAACCACAAATGTAACAAAGGCAACAGCCCAAGCTGATGTGCTTATGATGACTCAAAATCCAGGAGGAAAGGAAAGAACTAGAGATGAATTTAAGAGCCTAGCAACCAAAGCTGGGTTTAAACATGTCATATTCCAATGTTTTGTATCTAATCTTTGGGTTATAGAGTTTCTTAAAAATTAATTTATATATATTGTAATTGAATTTCTATGTTTCTATGTCCTTTTGGTTGCTAAACTTTCTGCACTCCAAAACGTTGAATAAAAAAGTTGTTATTGTTGTTATAA

Coding sequence (CDS)

ATGATATGTGAGAGATCTGACTTAAGAGACAGTCTTCTTAAGCTGTCATCTGGATTCATGGACCTTGATTCTTCTCTGAAATCGATTGCATCAATTTCCTTTAATATCATAGTTTTGTGTTTGACCATGGAGTTCATTTTCTTCTTTTATAAAGTTGGTATTAAGCCTGAAGGGAGAAGGGCCCCATCTGATATCTGTCGATTCAAGGACTATAGAGTGAAATCTATTGATCCGGGAGAGCACTGGATTAGTTCTGAGATTGGACCAAGTGAACTTGATGTTGGACAGAGGAGGGAGCGTCTTGGGGTGCTATCTAAGGTACCTTTTGGGGTGAATGAGGGTGTAGTTTTGGCTTCAACTTCAACTTTTGGCGTGATCGTTGAAAGAAAGGAAACTCAATCACTTTTGAAGAACCGTATTATACTTGGAGTAGCAATCAGATTTTTGAGTGTGTTCCTAACCCATTCTAGTTCCGAGGTTACTTCCATAGAGAATGTCTTATGTGCTCCCACTTCAGTTAGCCAGAGTGAGTTGAAGAATGCAGTGATTGAAGGAGGAGTTCCTTTTGACCGAGCTCATGGAGGAGTTCATGCATTTGAATACCCTAAGTTAGATCCCAGATTCAACCAAGTATTTAACATTGCAATGATAAATCACACTACAATGTCCATAAAGAAAATTGTGGAATCATACAAAGGTTTTGCAAACATCAAGCAATTAGTTGATGTTGGTGGTGGCCTTGGGGTCACCCTTCAAATCATCACTTCTACCTATCCTTCCATCAAAGGCATCAATTTCGACTTACCTCATGTCATCCGTGATGCCCCTGCTTATAATAGGGTTCAACATGTGGGAGGAGACATGTTTGAGAGTGTTCCAAATGGAGATGCTATTTTCATGAAGTGGATATTACATGATTGGAGCGACGATCATTGCACAAAGTTATTGAAAAATTGCTATAATGCAATTCCAGATGATGGAAAGATAATTATAGTAGACAGTGTGATTCCCACGGAGCCTGAAACCACAAATGTAACAAAGGCAACAGCCCAAGCTGATGTGCTTATGATGACTCAAAATCCAGGAGGAAAGGAAAGAACTAGAGATGAATTTAAGAGCCTAGCAACCAAAGCTGGGTTTAAACATGTCATATTCCAATGTTTTGTATCTAATCTTTGGGTTATAGAGTTTCTTAAAAATTAA

Protein sequence

MICERSDLRDSLLKLSSGFMDLDSSLKSIASISFNIIVLCLTMEFIFFFYKVGIKPEGRRAPSDICRFKDYRVKSIDPGEHWISSEIGPSELDVGQRRERLGVLSKVPFGVNEGVVLASTSTFGVIVERKETQSLLKNRIILGVAIRFLSVFLTHSSSEVTSIENVLCAPTSVSQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN*
Homology
BLAST of CSPI03G04030 vs. ExPASy Swiss-Prot
Match: Q8LL87 (Caffeic acid 3-O-methyltransferase OS=Coffea canephora OX=49390 GN=COMT PE=2 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 6.8e-77
Identity = 137/251 (54.58%), Postives = 181/251 (72.11%), Query Frame = 0

Query: 152 FLTHSSSEVTSIENVLCAPTSV---SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRF 211
           FLT ++  V+    +L     V   S   LK+AV++GG+PF++A+ G+ AFEY   DPRF
Sbjct: 101 FLTKNADGVSMAPLLLMNQDKVLMESWYHLKDAVLDGGIPFNKAY-GMTAFEYHGTDPRF 160

Query: 212 NQVFNIAMINHTTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDL 271
           N+VFN  M NH+T+++KKI+E Y+GF  +K +VDVGGG G TL +I S YP+IKGINF+L
Sbjct: 161 NKVFNQGMSNHSTITMKKILEVYRGFEGLKTVVDVGGGTGATLNMIISKYPTIKGINFEL 220

Query: 272 PHVIRDAPAYNRVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGK 331
           PHV+ DAP+++ V+HVGGDMF SVP GDAIFMKWI HDWSDDHC KLLKNCY A+PD+GK
Sbjct: 221 PHVVEDAPSHSGVEHVGGDMFVSVPKGDAIFMKWICHDWSDDHCRKLLKNCYQALPDNGK 280

Query: 332 IIIVDSVIPTEPETTNVTKATAQADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCF 391
           +I+ + V+P  P+T+  T+     DV+M+  NPGGKERT  EF++LA  AGFK     C 
Sbjct: 281 VILAECVLPEAPDTSLATQNVVHVDVVMLAHNPGGKERTEKEFEALAKGAGFKEFRKVCS 340

Query: 392 VSNLWVIEFLK 400
             N W++E  K
Sbjct: 341 AVNTWIMELCK 350

BLAST of CSPI03G04030 vs. ExPASy Swiss-Prot
Match: Q9XGW0 (Caffeic acid 3-O-methyltransferase 1 OS=Ocimum basilicum OX=39350 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 8.9e-77
Identity = 139/251 (55.38%), Postives = 175/251 (69.72%), Query Frame = 0

Query: 152 FLTHSSSEVTSIENVLCAPTSV---SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRF 211
           FLT +   V+     L     V   S   L +AV++GG+PF++A+ G+ AFEY   DPRF
Sbjct: 112 FLTKNEDGVSMAPLTLMNQDKVLMESWYHLSDAVVDGGIPFNKAY-GMTAFEYHGTDPRF 171

Query: 212 NQVFNIAMINHTTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDL 271
           N+VFN  M NH+T+++KKI+E+Y GF  +K +VDVGGG G TL +I S YPSIKGINFDL
Sbjct: 172 NKVFNQGMSNHSTITMKKILETYTGFDGLKTVVDVGGGTGATLNMIVSKYPSIKGINFDL 231

Query: 272 PHVIRDAPAYNRVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGK 331
           PHVI DAP+Y  V+HVGGDMF SVP GDAIFMKWI HDWSD+HC K LKNCY+A+P +GK
Sbjct: 232 PHVIEDAPSYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYDALPQNGK 291

Query: 332 IIIVDSVIPTEPETTNVTKATAQADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCF 391
           +I+ + V+P  P+T   TK     DV+M+  NPGGKERT  EF+ LA  AGFK     C 
Sbjct: 292 VILAECVLPEAPDTGLATKNVVHIDVIMLAHNPGGKERTEKEFQGLAKAAGFKQFNKACC 351

Query: 392 VSNLWVIEFLK 400
             N W++E LK
Sbjct: 352 AYNTWIMELLK 361

BLAST of CSPI03G04030 vs. ExPASy Swiss-Prot
Match: Q43609 (Caffeic acid 3-O-methyltransferase OS=Prunus dulcis OX=3755 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.2e-76
Identity = 132/222 (59.46%), Postives = 168/222 (75.68%), Query Frame = 0

Query: 178 LKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKGFANI 237
           LK+AV+EGG+PF++A+ G+ AFEY   DPRFN+VFN  M +H+T+++KKI+E+YKGF  +
Sbjct: 143 LKDAVLEGGIPFNKAY-GMTAFEYHGTDPRFNKVFNRGMADHSTITMKKILETYKGFEGL 202

Query: 238 KQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVPNGDA 297
             +VDVGGG G  L +I S YPSIKGINFDLPHVI DAP Y  V+HVGGDMF SVP GDA
Sbjct: 203 TSVVDVGGGTGAVLNMIVSKYPSIKGINFDLPHVIEDAPQYPGVEHVGGDMFVSVPKGDA 262

Query: 298 IFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQADVLMM 357
           IFMKWI HDWSD+HC K LKNCY A+PD+GK+I+ + ++P  P+++  TK     DV+M+
Sbjct: 263 IFMKWICHDWSDEHCLKFLKNCYAALPDNGKVILGECILPVAPDSSLATKGVVHIDVIML 322

Query: 358 TQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
             NPGGKERT  EF++LA  AGF+     C   N +VIEFLK
Sbjct: 323 AHNPGGKERTEQEFQALAKGAGFQGFNVACSAFNTYVIEFLK 363

BLAST of CSPI03G04030 vs. ExPASy Swiss-Prot
Match: Q8GU25 (Caffeic acid 3-O-methyltransferase OS=Rosa chinensis OX=74649 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.0e-76
Identity = 130/222 (58.56%), Postives = 169/222 (76.13%), Query Frame = 0

Query: 178 LKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKGFANI 237
           LK+AV++GG+PF++A+ G+ AF+Y   DPRFN+VFN  M +H+T+++KKI+E+YKGF  +
Sbjct: 143 LKDAVLDGGIPFNKAY-GMTAFDYHGTDPRFNKVFNKGMADHSTITMKKILETYKGFEGL 202

Query: 238 KQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVPNGDA 297
             +VDVGGG G  + +I S YPSIKGINFDLPHVI DAP Y  VQHVGGDMF SVP GDA
Sbjct: 203 TSIVDVGGGTGAVVNMIVSKYPSIKGINFDLPHVIEDAPQYPGVQHVGGDMFVSVPKGDA 262

Query: 298 IFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQADVLMM 357
           IFMKWI HDWSD+HC K LKNCY A+PD+GK+I+ + ++P  P+T+  TK     DV+M+
Sbjct: 263 IFMKWICHDWSDEHCLKFLKNCYAALPDNGKVILGECILPVAPDTSLATKGVVHIDVVML 322

Query: 358 TQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
             NPGGKERT  EF++LA  +GF+ +   C   N +VIEFLK
Sbjct: 323 AHNPGGKERTEQEFEALAKGSGFQGIRVACNAFNTYVIEFLK 363

BLAST of CSPI03G04030 vs. ExPASy Swiss-Prot
Match: Q8W013 (Caffeic acid 3-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.8e-76
Identity = 139/251 (55.38%), Postives = 178/251 (70.92%), Query Frame = 0

Query: 152 FLTHSSSEVTSIENVLCAPTSV---SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRF 211
           FLT +   V+    +L     V   S   LK+AV+EGG+PF++A+ G+ AFEY   DPRF
Sbjct: 114 FLTKNEDGVSMAALLLMNQDKVLMESWYHLKDAVLEGGIPFNKAY-GMTAFEYHGKDPRF 173

Query: 212 NQVFNIAMINHTTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDL 271
           N+VFN  M NH+T+ +KKI+E Y+GF  +K +VDVGGG G TL +I S YPSIKGINFDL
Sbjct: 174 NKVFNQGMSNHSTIIMKKILEIYQGFQGLKTVVDVGGGTGATLNMIVSKYPSIKGINFDL 233

Query: 272 PHVIRDAPAYNRVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGK 331
           PHVI DAP+Y  V HVGGDMF SVP GDAIFMKWI HDWSD HC K LKNC+ A+P++GK
Sbjct: 234 PHVIEDAPSYPGVDHVGGDMFVSVPKGDAIFMKWICHDWSDAHCLKFLKNCHEALPENGK 293

Query: 332 IIIVDSVIPTEPETTNVTKATAQADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCF 391
           +I+ + ++P  P++T  T+ T   DV+M+  NPGGKERT  EF++LA  AGF+  I  C 
Sbjct: 294 VILAECLLPEAPDSTLSTQNTVHVDVIMLAHNPGGKERTEKEFEALAKGAGFRGFIKVCC 353

Query: 392 VSNLWVIEFLK 400
             N W++E LK
Sbjct: 354 AYNSWIMELLK 363

BLAST of CSPI03G04030 vs. ExPASy TrEMBL
Match: A0A5D3CGD2 (Caffeic acid 3-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold404G00010 PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 3.4e-119
Identity = 206/229 (89.96%), Postives = 219/229 (95.63%), Query Frame = 0

Query: 172 SVSQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESY 231
           +V +SELKNAVIEGGVPFDRAHGGVHAFEYP LDPRFNQVFNI MINHTTMSIK+IV+SY
Sbjct: 95  NVQRSELKNAVIEGGVPFDRAHGGVHAFEYPSLDPRFNQVFNIGMINHTTMSIKEIVKSY 154

Query: 232 KGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFES 291
           KGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAP+Y+ V+HVGGDMFE+
Sbjct: 155 KGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYHGVEHVGGDMFEN 214

Query: 292 VPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQ 351
           VPNGDAIFMKWILHDWSDDHC KLLKNCYNAIPDDGK+IIV+ V PTEPETT VTK+ AQ
Sbjct: 215 VPNGDAIFMKWILHDWSDDHCLKLLKNCYNAIPDDGKVIIVECVFPTEPETTTVTKSIAQ 274

Query: 352 ADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN 401
            DVLMMTQNPGGKERTRDEFK+LATKAGFKH+IFQCFVSNLWVIEFLKN
Sbjct: 275 GDVLMMTQNPGGKERTRDEFKTLATKAGFKHLIFQCFVSNLWVIEFLKN 323

BLAST of CSPI03G04030 vs. ExPASy TrEMBL
Match: A0A1S3BM08 (caffeic acid 3-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103491014 PE=3 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 9.8e-119
Identity = 206/227 (90.75%), Postives = 217/227 (95.59%), Query Frame = 0

Query: 174 SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKG 233
           S SELKNAVIEGGVPFDRAHGGVHAFEYP LDPRFNQVFNI MINHTTMSIK+IV+SYKG
Sbjct: 133 SWSELKNAVIEGGVPFDRAHGGVHAFEYPSLDPRFNQVFNIGMINHTTMSIKEIVKSYKG 192

Query: 234 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVP 293
           FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAP+Y+ V+HVGGDMFE+VP
Sbjct: 193 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYHGVEHVGGDMFENVP 252

Query: 294 NGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQAD 353
           NGDAIFMKWILHDWSDDHC KLLKNCYNAIPDDGK+IIV+ V PTEPETT VTK+ AQ D
Sbjct: 253 NGDAIFMKWILHDWSDDHCLKLLKNCYNAIPDDGKVIIVECVFPTEPETTTVTKSIAQGD 312

Query: 354 VLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN 401
           VLMMTQNPGGKERTRDEFK+LATKAGFKH+IFQCFVSNLWVIEFLKN
Sbjct: 313 VLMMTQNPGGKERTRDEFKTLATKAGFKHLIFQCFVSNLWVIEFLKN 359

BLAST of CSPI03G04030 vs. ExPASy TrEMBL
Match: A0A6J1CQX8 (caffeic acid 3-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111013400 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 1.2e-103
Identity = 182/226 (80.53%), Postives = 198/226 (87.61%), Query Frame = 0

Query: 174 SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKG 233
           S  ELKNAVIEGGV F+RAHGGVHAFEYP LDPRFN+VFN AMINHTTM IKKIVESYKG
Sbjct: 138 SWRELKNAVIEGGVAFNRAHGGVHAFEYPGLDPRFNKVFNTAMINHTTMGIKKIVESYKG 197

Query: 234 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVP 293
           F N+ QLVDVGGGLGVTLQ+ITS YPSIKGINFDLPHVIRDAPAY  V+H+GGDMFESVP
Sbjct: 198 FGNLNQLVDVGGGLGVTLQVITSKYPSIKGINFDLPHVIRDAPAYPGVEHIGGDMFESVP 257

Query: 294 NGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQAD 353
            GDAIFMKWILHDWSD+HC KLLKNCY AIP+DGK+I VD+V+P   ETT   K+  Q D
Sbjct: 258 KGDAIFMKWILHDWSDEHCVKLLKNCYAAIPNDGKVIAVDAVVPLVIETTITMKSITQGD 317

Query: 354 VLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
           VLMMTQNPGGKERTRDEFK+LATK GFK + F+CFV NLWVIEF+K
Sbjct: 318 VLMMTQNPGGKERTRDEFKALATKTGFKRINFECFVYNLWVIEFIK 363

BLAST of CSPI03G04030 vs. ExPASy TrEMBL
Match: A0A6J1FBC5 (caffeic acid 3-O-methyltransferase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111443875 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 4.4e-103
Identity = 177/226 (78.32%), Postives = 198/226 (87.61%), Query Frame = 0

Query: 174 SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKG 233
           S SELKNAVIEGG+PF+R HGGVHAFEYP+LD RFNQVFN  MINHTTM IK +VESY G
Sbjct: 144 SWSELKNAVIEGGIPFNRTHGGVHAFEYPRLDQRFNQVFNNGMINHTTMVIKVVVESYNG 203

Query: 234 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVP 293
           FAN+KQLVDVGGGLGVTL+IITS YPSIKGINFDLPHVI DAP Y  V+HVGGDMFE VP
Sbjct: 204 FANLKQLVDVGGGLGVTLKIITSAYPSIKGINFDLPHVIEDAPFYPGVEHVGGDMFEKVP 263

Query: 294 NGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQAD 353
            GDAIFMKWILHDWSD+HC KLLKNCY+AIPDDGK+I+++ V+PT PETT   KA +Q D
Sbjct: 264 KGDAIFMKWILHDWSDEHCVKLLKNCYSAIPDDGKVIVMEGVVPTVPETTAAEKAISQGD 323

Query: 354 VLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
           +LMMTQNPGGKER+RDEFK+LATKAGFKH+   CF+ N W+IEFLK
Sbjct: 324 LLMMTQNPGGKERSRDEFKALATKAGFKHIKLVCFLCNFWIIEFLK 369

BLAST of CSPI03G04030 vs. ExPASy TrEMBL
Match: A0A6J1L279 (caffeic acid 3-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498431 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 1.1e-101
Identity = 176/226 (77.88%), Postives = 202/226 (89.38%), Query Frame = 0

Query: 174 SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKG 233
           S SELKNAVIEGGVPF+RAHGGVHAFEYP+LD RFNQVFN AMIN++T +IK +V+ Y G
Sbjct: 144 SWSELKNAVIEGGVPFNRAHGGVHAFEYPRLDQRFNQVFNNAMINYSTTAIKAVVKCYNG 203

Query: 234 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVP 293
           FA++KQLVDVGGGLGVTL+IITS YPSIKGINFDLPHVI+DAP+Y  V+HVGGDMFE VP
Sbjct: 204 FASLKQLVDVGGGLGVTLKIITSAYPSIKGINFDLPHVIQDAPSYPGVEHVGGDMFEKVP 263

Query: 294 NGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQAD 353
            GDAIF+KWILHDWSD+HC KLLKNCY AIPDDGK+I+++ V+PT PET++V K   Q D
Sbjct: 264 EGDAIFIKWILHDWSDEHCEKLLKNCYAAIPDDGKVIVMEGVLPTVPETSDVAKGIIQGD 323

Query: 354 VLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
           VLMMTQNPGGKERTRDEF++LATKAGFKHV F CF+SN W+IEFLK
Sbjct: 324 VLMMTQNPGGKERTRDEFEALATKAGFKHVKFVCFLSNHWIIEFLK 369

BLAST of CSPI03G04030 vs. NCBI nr
Match: XP_004150935.3 (caffeic acid 3-O-methyltransferase [Cucumis sativus] >KAE8650102.1 hypothetical protein Csa_010461 [Cucumis sativus])

HSP 1 Score: 446.8 bits (1148), Expect = 1.9e-121
Identity = 213/227 (93.83%), Postives = 220/227 (96.92%), Query Frame = 0

Query: 174 SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKG 233
           S SELKNAV EGGVPFDRAHGGV+AFEYPKLDPRFNQVFNIAM+NHTTMSIKKIVESYKG
Sbjct: 135 SWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIVESYKG 194

Query: 234 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVP 293
           FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN VQHVGGDMFESVP
Sbjct: 195 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDMFESVP 254

Query: 294 NGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQAD 353
           NGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIV+SVIPT+PE TNVTKAT Q D
Sbjct: 255 NGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNVTKATTQGD 314

Query: 354 VLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN 401
           VLMMTQNPGGKERTRDEFKSLATKAGFKHV+F+CFV+NLWVIE LKN
Sbjct: 315 VLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLKN 361

BLAST of CSPI03G04030 vs. NCBI nr
Match: KAE8650103.1 (hypothetical protein Csa_010827 [Cucumis sativus])

HSP 1 Score: 441.8 bits (1135), Expect = 6.3e-120
Identity = 219/238 (92.02%), Postives = 220/238 (92.44%), Query Frame = 0

Query: 174 SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKG 233
           S SELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTT+SIKKIVESYKG
Sbjct: 135 SWSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTVSIKKIVESYKG 194

Query: 234 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDA-----------PAYNRVQ 293
           FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDA                VQ
Sbjct: 195 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDALLIMFTKLNFDYELAGVQ 254

Query: 294 HVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPET 353
           HVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIP EPET
Sbjct: 255 HVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPMEPET 314

Query: 354 TNVTKATAQADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN 401
           TNVTKATAQADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN
Sbjct: 315 TNVTKATAQADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN 372

BLAST of CSPI03G04030 vs. NCBI nr
Match: KAA0038857.1 (caffeic acid 3-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK09426.1 caffeic acid 3-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 438.3 bits (1126), Expect = 6.9e-119
Identity = 206/229 (89.96%), Postives = 219/229 (95.63%), Query Frame = 0

Query: 172 SVSQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESY 231
           +V +SELKNAVIEGGVPFDRAHGGVHAFEYP LDPRFNQVFNI MINHTTMSIK+IV+SY
Sbjct: 95  NVQRSELKNAVIEGGVPFDRAHGGVHAFEYPSLDPRFNQVFNIGMINHTTMSIKEIVKSY 154

Query: 232 KGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFES 291
           KGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAP+Y+ V+HVGGDMFE+
Sbjct: 155 KGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYHGVEHVGGDMFEN 214

Query: 292 VPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQ 351
           VPNGDAIFMKWILHDWSDDHC KLLKNCYNAIPDDGK+IIV+ V PTEPETT VTK+ AQ
Sbjct: 215 VPNGDAIFMKWILHDWSDDHCLKLLKNCYNAIPDDGKVIIVECVFPTEPETTTVTKSIAQ 274

Query: 352 ADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN 401
            DVLMMTQNPGGKERTRDEFK+LATKAGFKH+IFQCFVSNLWVIEFLKN
Sbjct: 275 GDVLMMTQNPGGKERTRDEFKTLATKAGFKHLIFQCFVSNLWVIEFLKN 323

BLAST of CSPI03G04030 vs. NCBI nr
Match: XP_008449018.1 (PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 436.8 bits (1122), Expect = 2.0e-118
Identity = 206/227 (90.75%), Postives = 217/227 (95.59%), Query Frame = 0

Query: 174 SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKG 233
           S SELKNAVIEGGVPFDRAHGGVHAFEYP LDPRFNQVFNI MINHTTMSIK+IV+SYKG
Sbjct: 133 SWSELKNAVIEGGVPFDRAHGGVHAFEYPSLDPRFNQVFNIGMINHTTMSIKEIVKSYKG 192

Query: 234 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVP 293
           FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAP+Y+ V+HVGGDMFE+VP
Sbjct: 193 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYHGVEHVGGDMFENVP 252

Query: 294 NGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQAD 353
           NGDAIFMKWILHDWSDDHC KLLKNCYNAIPDDGK+IIV+ V PTEPETT VTK+ AQ D
Sbjct: 253 NGDAIFMKWILHDWSDDHCLKLLKNCYNAIPDDGKVIIVECVFPTEPETTTVTKSIAQGD 312

Query: 354 VLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN 401
           VLMMTQNPGGKERTRDEFK+LATKAGFKH+IFQCFVSNLWVIEFLKN
Sbjct: 313 VLMMTQNPGGKERTRDEFKTLATKAGFKHLIFQCFVSNLWVIEFLKN 359

BLAST of CSPI03G04030 vs. NCBI nr
Match: XP_038903769.1 (caffeic acid 3-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 414.8 bits (1065), Expect = 8.2e-112
Identity = 192/227 (84.58%), Postives = 211/227 (92.95%), Query Frame = 0

Query: 174 SQSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKG 233
           S SELK+AVIEGGVPF+RAHGGVHAFEYP LDPRFNQVFN  M+NHTTM IKK+V++Y G
Sbjct: 185 SWSELKHAVIEGGVPFNRAHGGVHAFEYPGLDPRFNQVFNTGMLNHTTMCIKKLVKTYNG 244

Query: 234 FANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVP 293
           FANI+QLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAP+Y  V+HVGGDMF+ VP
Sbjct: 245 FANIEQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYPGVEHVGGDMFQKVP 304

Query: 294 NGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQAD 353
           NGD +FMKWILHDWSDDHC KLLKNCY AIP+DGK+I+VDSV+PT PETTNVTK+ AQ D
Sbjct: 305 NGDVVFMKWILHDWSDDHCIKLLKNCYVAIPNDGKVIVVDSVVPTIPETTNVTKSIAQGD 364

Query: 354 VLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLKN 401
           VLMMTQNPGGKERTRDEFK++A KAGFKH+IFQCFVSNLWVIEFLKN
Sbjct: 365 VLMMTQNPGGKERTRDEFKAIAIKAGFKHIIFQCFVSNLWVIEFLKN 411

BLAST of CSPI03G04030 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 282.3 bits (721), Expect = 5.9e-76
Identity = 124/222 (55.86%), Postives = 170/222 (76.58%), Query Frame = 0

Query: 178 LKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKGFANI 237
           LK+A+++GG+PF++A+ G+ AFEY   DPRFN+VFN  M NH+T+++KKI+E+YKGF  +
Sbjct: 141 LKDAILDGGIPFNKAY-GMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFEGL 200

Query: 238 KQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVPNGDA 297
             LVDVGGG+G TL++I S YP++KGINFDLPHVI DAP++  ++HVGGDMF SVP GDA
Sbjct: 201 TSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDA 260

Query: 298 IFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATAQADVLMM 357
           IFMKWI HDWSD+HC K LKNCY ++P+DGK+I+ + ++P  P+++  TK     D +M+
Sbjct: 261 IFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILPETPDSSLSTKQVVHVDCIML 320

Query: 358 TQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
             NPGGKERT  EF++LA  +GFK +   C    + +IE LK
Sbjct: 321 AHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLK 361

BLAST of CSPI03G04030 vs. TAIR 10
Match: AT1G21100.1 (O-methyltransferase family protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.4e-61
Identity = 110/225 (48.89%), Postives = 154/225 (68.44%), Query Frame = 0

Query: 176 SELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKGFA 235
           ++LK+ V+EGG  F RAHGG+  F+Y   D RF+++FN      T   +KK +E Y+GF 
Sbjct: 151 AQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFN--QTGFTIAVVKKALEVYEGFK 210

Query: 236 NIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVPNG 295
            +K LVDVGGG+G TL ++TS YP+IKGINFDL   +  AP+Y  V+HV GDMF  VP G
Sbjct: 211 GVKVLVDVGGGVGNTLGVVTSKYPNIKGINFDLTCALAQAPSYPGVEHVAGDMFVDVPTG 270

Query: 296 DAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATA-QADV 355
           DA+ +K ILHDW+D+ C K+LKNC+ ++P++GK+++++ V P E E  ++    A   D+
Sbjct: 271 DAMILKRILHDWTDEDCVKILKNCWKSLPENGKVVVIELVTPDEAENGDINANIAFDMDM 330

Query: 356 LMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
           LM TQ  GGKER+R EF++LA  +GF H  F C   + W+IEF K
Sbjct: 331 LMFTQCSGGKERSRAEFEALAAASGFTHCKFVCQAYHCWIIEFCK 373

BLAST of CSPI03G04030 vs. TAIR 10
Match: AT1G21130.1 (O-methyltransferase family protein )

HSP 1 Score: 230.3 bits (586), Expect = 2.7e-60
Identity = 108/225 (48.00%), Postives = 151/225 (67.11%), Query Frame = 0

Query: 176 SELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKGFA 235
           ++LK+ V+EGG  F RAHGG+  F+Y   D RF+++FN      T   +KK +E Y+GF 
Sbjct: 151 AQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFN--QTGFTIAVVKKALEVYQGFK 210

Query: 236 NIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVPNG 295
            +  LVDVGGG+G TL ++ S YP+IKGINFDL   +  AP+Y  V+HV GDMF  VP G
Sbjct: 211 GVNVLVDVGGGVGNTLGVVASKYPNIKGINFDLTCALAQAPSYPGVEHVAGDMFVDVPTG 270

Query: 296 DAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATA-QADV 355
           DA+ +K ILHDW+D+ C K+LKNC+ ++P+ GK+++++ V P E E  ++    A   D+
Sbjct: 271 DAMILKRILHDWTDEDCVKILKNCWKSLPESGKVVVIELVTPDEAENGDINANIAFDMDM 330

Query: 356 LMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
           LM TQ  GGKER+R EF++LA  +GF H  F C   + W+IEF K
Sbjct: 331 LMFTQCSGGKERSRAEFEALAAASGFTHCKFVCQAYHCWIIEFCK 373

BLAST of CSPI03G04030 vs. TAIR 10
Match: AT1G21110.1 (O-methyltransferase family protein )

HSP 1 Score: 229.9 bits (585), Expect = 3.5e-60
Identity = 108/225 (48.00%), Postives = 151/225 (67.11%), Query Frame = 0

Query: 176 SELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKGFA 235
           ++LK+ V+EGG  F RAHGG+  F+Y   D RF+++FN      T   +KK +E Y+GF 
Sbjct: 151 AQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFN--QTGFTIAVVKKALEVYQGFK 210

Query: 236 NIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVPNG 295
            +  LVDVGGG+G TL ++TS YP+IKGINFDL   +  AP Y  V+HV GDMF  VP G
Sbjct: 211 GVNVLVDVGGGVGNTLGVVTSKYPNIKGINFDLTCALAQAPTYPGVEHVAGDMFVDVPTG 270

Query: 296 DAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATA-QADV 355
           +A+ +K ILHDW+D+ C K+LKNC+ ++P +GK+++++ V P E E  ++    A   D+
Sbjct: 271 NAMILKRILHDWTDEDCVKILKNCWKSLPQNGKVVVIELVTPDEAENGDINANIAFDMDM 330

Query: 356 LMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
           LM TQ  GGKER+R EF++LA  +GF H  F C   + W+IEF K
Sbjct: 331 LMFTQCSGGKERSRAEFEALAAASGFSHCQFVCQAYHCWIIEFCK 373

BLAST of CSPI03G04030 vs. TAIR 10
Match: AT1G21120.1 (O-methyltransferase family protein )

HSP 1 Score: 229.2 bits (583), Expect = 5.9e-60
Identity = 108/225 (48.00%), Postives = 152/225 (67.56%), Query Frame = 0

Query: 176 SELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINHTTMSIKKIVESYKGFA 235
           ++LK+ V+EGG  F RAHGG+  F+Y   D RF+++FN      T   +KK +E Y+GF 
Sbjct: 151 AQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFN--QTGFTIAVVKKALEVYQGFK 210

Query: 236 NIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNRVQHVGGDMFESVPNG 295
            +  LVDVGGG+G TL ++TS YP+IKGINFDL   +  AP+Y  V+HV GDMF  VP G
Sbjct: 211 GVNVLVDVGGGVGNTLGVVTSKYPNIKGINFDLTCALAQAPSYPGVEHVAGDMFVDVPTG 270

Query: 296 DAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVDSVIPTEPETTNVTKATA-QADV 355
           DA+ +K ILHDW+D+ C K+LKNC+ ++P++GK+++++ V P E E  ++    A   D+
Sbjct: 271 DAMILKRILHDWTDEDCVKILKNCWKSLPENGKVVVIELVTPDEAENGDINANIAFDMDM 330

Query: 356 LMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCFVSNLWVIEFLK 400
           LM TQ  GGKER+R EF++LA  + F H  F C   + W+IEF K
Sbjct: 331 LMFTQCSGGKERSRAEFEALAAASCFTHCKFVCQAYHCWIIEFCK 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LL876.8e-7754.58Caffeic acid 3-O-methyltransferase OS=Coffea canephora OX=49390 GN=COMT PE=2 SV=... [more]
Q9XGW08.9e-7755.38Caffeic acid 3-O-methyltransferase 1 OS=Ocimum basilicum OX=39350 GN=COMT1 PE=2 ... [more]
Q436091.2e-7659.46Caffeic acid 3-O-methyltransferase OS=Prunus dulcis OX=3755 GN=COMT1 PE=2 SV=1[more]
Q8GU252.0e-7658.56Caffeic acid 3-O-methyltransferase OS=Rosa chinensis OX=74649 GN=COMT1 PE=2 SV=1[more]
Q8W0135.8e-7655.38Caffeic acid 3-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=COMT1 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A5D3CGD23.4e-11989.96Caffeic acid 3-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BM089.8e-11990.75caffeic acid 3-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103491014 ... [more]
A0A6J1CQX81.2e-10380.53caffeic acid 3-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1FBC54.4e-10378.32caffeic acid 3-O-methyltransferase-like isoform X3 OS=Cucurbita moschata OX=3662... [more]
A0A6J1L2791.1e-10177.88caffeic acid 3-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
XP_004150935.31.9e-12193.83caffeic acid 3-O-methyltransferase [Cucumis sativus] >KAE8650102.1 hypothetical ... [more]
KAE8650103.16.3e-12092.02hypothetical protein Csa_010827 [Cucumis sativus][more]
KAA0038857.16.9e-11989.96caffeic acid 3-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK09426.1 c... [more]
XP_008449018.12.0e-11890.75PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis melo][more]
XP_038903769.18.2e-11284.58caffeic acid 3-O-methyltransferase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G54160.15.9e-7655.86O-methyltransferase 1 [more]
AT1G21100.11.4e-6148.89O-methyltransferase family protein [more]
AT1G21130.12.7e-6048.00O-methyltransferase family protein [more]
AT1G21110.13.5e-6048.00O-methyltransferase family protein [more]
AT1G21120.15.9e-6048.00O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 209..388
e-value: 2.4E-90
score: 305.0
NoneNo IPR availablePANTHERPTHR11746:SF169CAFFEIC ACID O-METHYLTRANSFERASEcoord: 173..399
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 176..381
e-value: 1.3E-65
score: 220.7
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 189..227
e-value: 2.4E-90
score: 305.0
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 173..399
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 177..399
score: 49.638351
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 176..397

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G04030.1CSPI03G04030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity