CSPI03G04000 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G04000
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	caffeic acid 3-O-methyltransferase-like
Location	Chr3: 3301640 .. 3306204 (-)
RNA-Seq Expression	CSPI03G04000
Synteny	CSPI03G04000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTTACTTAGCTTGTCAACATAATCAGTTCAACTCAACATACATCCCTATGTTAGTCTTCGTATCCTTTTGTTGGTATCCATTTCAAGTATTTTCCTTTCCATCTTCTTTTTGCCTGCTTATAAAAATACAACAAACTTTCGTTGATATGCAAAACAATCACCCTACCAAAATCATTCTCCTCCCACCAGAAAAGGTTGCCACACCTCCGGCTAGAAAATGGCTTCCTCCGACAACGACACCGCCGCCGAACAGCAACAACATTATGCTTACGCAGGACACCTCGTTACCTTATCAGTCCTACCCATGACACTCCAAGCAGTGTTCGAGCTTGGTGTGTTCGAGATCTTGGCAAAGGCTGGGGATGGGGCCAACCTCTCTCCAGCAGAGATAGCGGCTGAGATAACCACTACAAACCCCAACGCAGCATTGATGCTTGATCGGATGTTGAGGCTTTTGGCATGCCACTCGGTGGTGGGATGCTCTATAGTTTCTGATAAAGATGGGAATGTGCAAAGGCTTTATAGCCTTACTCCGATTTCTAAATGTTATGTTCGAAATGAAGATGGTGTTTCGTTGGGTCCAATGTTATCATTGATTCAAGATAAAGTGTTTCTGCAAAGCTGGTTTGCTCTCCTTAATTTCTTTATGCTTTTTAATATATATTTTTTTCGTTTAATAGAAGGGAAATTAGTAGGAATAGCGAAGATAAAATATAAATGGAGATATGCTATATTAGTGAGAAATTATGTAAGTACAGCTATGTTTGAGAAATTAGAGACTTAGATGGTACAAAATAGGGCGGATAAAAATTGAGTTATAAGAGTGGATATTTAATTTTGAAATTAAAAAAGAAAGTTAGAAAAGTACCAAAAGCTTAGTGAGAAAAATATGGTAGTAGGAAAAGTTTTCTAATTAGGACAGTTAGAAAATTTTAGATGAAGTTTTTTTTCCAACAATTTGAGAAATATGGAGAGTCGAAAAATAATAAAAAAAATATTATAACATAATATTGTTTTCAAAGATAGAAAAATGAAATATAATATTTTTAAATATAGTAAAGTAATATATGGAGACTGTATTAAAGATACTATCCATTGCTGTCCATCCATAGAATGTGATACATTTTGTGTAATTATTTTTTACAGTTTTGTTATTAAAAGTAATCATTTGTGGATATATATACATATGTTTGAGAAAAAAATTAAATTAAAAAGAGGAATAAAATAGTTATATAAACAGACTTTAAGGATTTCTTTTATAGGAATGTTAAAATTTTATGATTGAAAATTTTGAGAATTTTTTTAAAGAAGAAAATTAAATATTGTTAAAAGAGAAAAAAAACTAATTAAGGCATGTTTGGTAGGCATGTTTGTATCGTAGCAGAGAATTCAAAAATTGGATTCCAATTTAAATAATTGTAAACTATTTGTAGTTAACTATTAAATTTAGTTGACAGTAAATTATTATTAATTTATTTACATAACATGTTTTATATTAAAAAAAATGCATATTTATTGTTTTGTAATATTCTATAGCTTATATTAAAATGGAAACTTTGGACCCCATATGGTTTTTTTTAGAGAAAGAGACTAGTGTCAAGAGTATGAAAGTCCCACCACCGAATTTGAAGCCCAGATGCTCTTAGTGAAAAAGAGCGGTGAAGTTGAGAGCTAACGAACTAGAAGAATAGTCTTTGAATATAGGTGATGGCGACTACACCACAAACCTATGTAGTTACAACAATCTTCCTAGAGATTAAGTAAGCTGTTTTGTTTATTAAGGAATATTCTTCAATTTCTTTCGGTCCAAATGCTCCAAAGACAGCTACCGTTGCATTTGTGATAATAATGTTTCTTGTGGTTGAAAAATTCTAATGTTAAAGGGACAACACAACTGACTTTAGATCGTTGAACGAGTGTTGATACCCAATTTAAGCCCGTAAGGTATCCCAAATGAAGTTTGTCGCTCTACAGTTTACAAGTAAATGATCACTTCTTTCACAATGAGAGTGGCACATGACACACCAATTTGGATTTAAACAAGTTTTTGGCGGTCTTCTTCGAAGAACTTCCATTGTGTTGATACCTTTATGAAGAATGGACCAAAGGAAGAATTTGCATTTCTTTAGAATAGAAGATTTCCAAAGAGCTTTGAAAATCTGACGATCGACTTCATTATTCCCATCGGGAGGGGAAATTTGGATGGACTTTTCAAATGAAATCGTGGTGAATACCTTGTTGTTTTCCAGCTTCCAAATCGGTTTGCACATACCTTCGTCTACATTCGAATAAGGGAACTCTGAAGAGATTTGATTCCAGAGCTCAATTTCTCTATCATTTAGCAGCCACACGGCTTTAAGTCCCAATTCTTATCCACTAGGTTCCATATCTCTTTTACTGAATTGTTTTTGGCTTCAGAGAGAGCGTATAATCTCGGGAGGGTGACAAAGGAAGTAATGGACAAAATCGTGGATTGACTTCCAGTCTAGATTTTCAATAAAGATCAAGTCTTTTTTTATCTCCATTAAAGAGAGCTCAAAAGAATCTGATGAAAGTATCTGCAATTTTTTCGTTAGTCGTATGGGAGGTACCTGTCTCGTCTTTGAATTCAGAAATGAAATTCCTCTTTTGATTTACCGAACAAATACGATAAAAGAAGTTGGTGTTTTCATTCTTGTTTTTGATGAGAAGAAGAAGTAGAAGTAGTGTGTGTACCTTTTAAAGCAGATGCATGGAGTTTTGTGTCATCATGAGAGGAGTAAATGCCCTTAGTTGTTGAATAGTAATTGTGAAACGTCGTCATTTTTCAATATCAATGGCTGCTTCACTGGAAGTTCCGACGGGGGAGTTTACTGTTGCGACGGATGGGATTCTTGGAGAGAGGAGAGAGGAGAAATCAAGCGCATATATGGTTTGAACTAAGTTAAAATTTATGTCTCATGCTTTATAATTAAAATTAATCTCATTAGGTTTGATAGAAACTTCATAATTAAGTAGTACCTATTGTATGGACTATTCAAGATGATTTAACCAACCATAAGAAACTAAGACTTTGTTTGGTAATCTTCTTTTTTTTTTTTAATTAAATCTATAGATATTATTTCTACCTCTCAATTTCTTTATTTGTCATCTTGTACTTACCTATTGTTTAAAAAATTAAACCAAATTTTAACAACTAAAAAAACACTTCTAAAAACTTAGTTTTTTTTTTTTTTTTTTAATTTGACTAATAATTTAACGATGTACTTGAGAAAAAATGTAAATCATTATAAAAAATGGAGAGGAAATATTCTAAATTTTAAAATCACGTGAACAAATTAACTTGGATTGTGAACCAAACATAACTGAGTTTTTTTGAAATTGTAGGAGTGAGTTGAAGAATGCAGTAACTGAAGGAGGAGTTCCTTTTGACCGAGCTCATGGAGGAGTTAATGCATTTGAATACCCTAAGTTAGATCCAAGATTCAACCAAGTATTTAACATTGCAATGGTAAATCACACTACAATGTCCATAAAGAAAATTGTGGAATCATACAAAGGTTTTGCAAACATCAAGCAATTAGTTGATGTTGGTGGTGGCCTTGGGGTCACCCTTCAAATCATCACTTCTACCTATCCTTCCATCAAAGGCATCAATTTCGACTTACCTCATGTCATCCGTGATGCCCCTGCTTATAATGGTAAGTAGTTCCAATCATTTAATTTGAAAAAGTTTACAAAGCTGAATTTTGATTATGAATTGGCAGGGGTTCAACATGTGGGAGGAGACATGTTTGAGAGTGTTCCAAATGGAGATGCTATTTTCATGAAGGTTAGTTTTCTTTTCATTTTCATAAACAAAAACATTCATAAACAATAGTTCCTATGCTAGAGACTATTTTTTTTAGGACTTTATCGAATTTTAGAGACTATTCATAAGATTTTATATATAAAAAAGAATCTAACTTTTAACTTTTGAATTTGTAATTTAACCAATAATAATTAACCTATAGTTGAGAGATATAGGTCACTCTGCCCTTTGAAAACCGTCAACATTTAAGATTGTACAATTAGTATTTTATGGACTAAGTCATACTTGAAATGACAAAATTCTTTTATTTTATTGTTATTATAGTGGATACTACATGATTGGAGCGACGATCATTGCACAAAGTTATTGAAAAATTGCTATAATGCAATTCCAGATGATGGAAAGATAATCATAGTAGAGAGTGTGATTCCCACGAAGCCTGAAATCACCAATGTAACAAAGGCAACAACTCAAGGTGATGTGCTTATGATGACTCAAAATCCAGGAGGAAAGGAAAGAACTAGAGATGAATTTAAGAGCCTAGCAACCAAAGCTGGGTTTAAACATGTCATGTTCGAATGTTTTGTTGCTAATCTTTGGGTTATAGAGTTACTTAAAAATTAACTTTTATATATTGCATGCACCAAACTTATTGATGGTTTAGTTACTACTGTATTGTCATTGAATTTCTATGTTTCTATGTTTCTATGTCCTTTTGGTTGCTGAACTTTCTGCCCTCCAAAAGGTTGAATAAAAAAGTTGTTATTGTTGTTA

mRNA sequence

AGTTACTTAGCTTGTCAACATAATCAGTTCAACTCAACATACATCCCTATGTTAGTCTTCGTATCCTTTTGTTGGTATCCATTTCAAGTATTTTCCTTTCCATCTTCTTTTTGCCTGCTTATAAAAATACAACAAACTTTCGTTGATATGCAAAACAATCACCCTACCAAAATCATTCTCCTCCCACCAGAAAAGGTTGCCACACCTCCGGCTAGAAAATGGCTTCCTCCGACAACGACACCGCCGCCGAACAGCAACAACATTATGCTTACGCAGGACACCTCGTTACCTTATCAGTCCTACCCATGACACTCCAAGCAGTGTTCGAGCTTGGTGTGTTCGAGATCTTGGCAAAGGCTGGGGATGGGGCCAACCTCTCTCCAGCAGAGATAGCGGCTGAGATAACCACTACAAACCCCAACGCAGCATTGATGCTTGATCGGATGTTGAGGCTTTTGGCATGCCACTCGGTGGTGGGATGCTCTATAGTTTCTGATAAAGATGGGAATGTGCAAAGGCTTTATAGCCTTACTCCGATTTCTAAATGTTATGTTCGAAATGAAGATGGTGTTTCGTTGGGTCCAATGTTATCATTGATTCAAGATAAAGTGTTTCTGCAAAGCTGGAGTGAGTTGAAGAATGCAGTAACTGAAGGAGGAGTTCCTTTTGACCGAGCTCATGGAGGAGTTAATGCATTTGAATACCCTAAGTTAGATCCAAGATTCAACCAAGTATTTAACATTGCAATGGTAAATCACACTACAATGTCCATAAAGAAAATTGTGGAATCATACAAAGGTTTTGCAAACATCAAGCAATTAGTTGATGTTGGTGGTGGCCTTGGGGTCACCCTTCAAATCATCACTTCTACCTATCCTTCCATCAAAGGCATCAATTTCGACTTACCTCATGTCATCCGTGATGCCCCTGCTTATAATGGGGTTCAACATGTGGGAGGAGACATGTTTGAGAGTGTTCCAAATGGAGATGCTATTTTCATGAAGTGGATACTACATGATTGGAGCGACGATCATTGCACAAAGTTATTGAAAAATTGCTATAATGCAATTCCAGATGATGGAAAGATAATCATAGTAGAGAGTGTGATTCCCACGAAGCCTGAAATCACCAATGTAACAAAGGCAACAACTCAAGGTGATGTGCTTATGATGACTCAAAATCCAGGAGGAAAGGAAAGAACTAGAGATGAATTTAAGAGCCTAGCAACCAAAGCTGGGTTTAAACATGTCATGTTCGAATGTTTTGTTGCTAATCTTTGGGTTATAGAGTTACTTAAAAATTAACTTTTATATATTGCATGCACCAAACTTATTGATGGTTTAGTTACTACTGTATTGTCATTGAATTTCTATGTTTCTATGTTTCTATGTCCTTTTGGTTGCTGAACTTTCTGCCCTCCAAAAGGTTGAATAAAAAAGTTGTTATTGTTGTTA

Coding sequence (CDS)

ATGGCTTCCTCCGACAACGACACCGCCGCCGAACAGCAACAACATTATGCTTACGCAGGACACCTCGTTACCTTATCAGTCCTACCCATGACACTCCAAGCAGTGTTCGAGCTTGGTGTGTTCGAGATCTTGGCAAAGGCTGGGGATGGGGCCAACCTCTCTCCAGCAGAGATAGCGGCTGAGATAACCACTACAAACCCCAACGCAGCATTGATGCTTGATCGGATGTTGAGGCTTTTGGCATGCCACTCGGTGGTGGGATGCTCTATAGTTTCTGATAAAGATGGGAATGTGCAAAGGCTTTATAGCCTTACTCCGATTTCTAAATGTTATGTTCGAAATGAAGATGGTGTTTCGTTGGGTCCAATGTTATCATTGATTCAAGATAAAGTGTTTCTGCAAAGCTGGAGTGAGTTGAAGAATGCAGTAACTGAAGGAGGAGTTCCTTTTGACCGAGCTCATGGAGGAGTTAATGCATTTGAATACCCTAAGTTAGATCCAAGATTCAACCAAGTATTTAACATTGCAATGGTAAATCACACTACAATGTCCATAAAGAAAATTGTGGAATCATACAAAGGTTTTGCAAACATCAAGCAATTAGTTGATGTTGGTGGTGGCCTTGGGGTCACCCTTCAAATCATCACTTCTACCTATCCTTCCATCAAAGGCATCAATTTCGACTTACCTCATGTCATCCGTGATGCCCCTGCTTATAATGGGGTTCAACATGTGGGAGGAGACATGTTTGAGAGTGTTCCAAATGGAGATGCTATTTTCATGAAGTGGATACTACATGATTGGAGCGACGATCATTGCACAAAGTTATTGAAAAATTGCTATAATGCAATTCCAGATGATGGAAAGATAATCATAGTAGAGAGTGTGATTCCCACGAAGCCTGAAATCACCAATGTAACAAAGGCAACAACTCAAGGTGATGTGCTTATGATGACTCAAAATCCAGGAGGAAAGGAAAGAACTAGAGATGAATTTAAGAGCCTAGCAACCAAAGCTGGGTTTAAACATGTCATGTTCGAATGTTTTGTTGCTAATCTTTGGGTTATAGAGTTACTTAAAAATTAA

Protein sequence

MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLKN*

Homology

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BLAST of CSPI03G04000 vs. ExPASy Swiss-Prot
Match: Q8LL87 (Caffeic acid 3-O-methyltransferase OS=Coffea canephora OX=49390 GN=COMT PE=2 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 9.7e-115
Identity = 192/349 (55.01%), Postives = 259/349 (74.21%), Query Frame = 0


Query: 12  QQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNAAL 71
           +++   +A  L + SVLPM L++  EL + E++AKAG GA +SP+E+AA++ T NP A +
Sbjct: 3   EEEACLFAMSLASASVLPMVLKSAIELDLLELIAKAGPGAYVSPSELAAQLPTHNPEAPI 62

Query: 72  MLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLIQDKV 131
           MLDR+LRLLA +SV+ C + +  DG V+RLY L P+ K   +N DGVS+ P+L + QDKV
Sbjct: 63  MLDRILRLLATYSVLDCKLNNLADGGVERLYGLAPVCKFLTKNADGVSMAPLLLMNQDKV 122

Query: 132 FLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIVES 191
            ++SW  LK+AV +GG+PF++A+ G+ AFEY   DPRFN+VFN  M NH+T+++KKI+E 
Sbjct: 123 LMESWYHLKDAVLDGGIPFNKAY-GMTAFEYHGTDPRFNKVFNQGMSNHSTITMKKILEV 182

Query: 192 YKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDMFE 251
           Y+GF  +K +VDVGGG G TL +I S YP+IKGINF+LPHV+ DAP+++GV+HVGGDMF 
Sbjct: 183 YRGFEGLKTVVDVGGGTGATLNMIISKYPTIKGINFELPHVVEDAPSHSGVEHVGGDMFV 242

Query: 252 SVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNVTKATT 311
           SVP GDAIFMKWI HDWSDDHC KLLKNCY A+PD+GK+I+ E V+P  P+ +  T+   
Sbjct: 243 SVPKGDAIFMKWICHDWSDDHCRKLLKNCYQALPDNGKVILAECVLPEAPDTSLATQNVV 302

Query: 312 QGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
             DV+M+  NPGGKERT  EF++LA  AGFK     C   N W++EL K
Sbjct: 303 HVDVVMLAHNPGGKERTEKEFEALAKGAGFKEFRKVCSAVNTWIMELCK 350

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BLAST of CSPI03G04000 vs. ExPASy Swiss-Prot
Match: Q9XGW0 (Caffeic acid 3-O-methyltransferase 1 OS=Ocimum basilicum OX=39350 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 4.8e-114
Identity = 189/351 (53.85%), Postives = 258/351 (73.50%), Query Frame = 0


Query: 10  AEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNA 69
           +++++++ +A  L + SVLPM L++  EL + E++ K+G GA +SP ++AA++ TTNP+A
Sbjct: 12  SDEEENFLFAMQLASASVLPMVLKSAIELDLLELIKKSGAGAFVSPVDLAAQLPTTNPDA 71

Query: 70  ALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLIQD 129
            +MLDR+LRLL  ++++ C + +  DG V+RLY L P+ K   +NEDGVS+ P+  + QD
Sbjct: 72  HVMLDRILRLLTSYAILECRLKTLPDGGVERLYGLAPVCKFLTKNEDGVSMAPLTLMNQD 131

Query: 130 KVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIV 189
           KV ++SW  L +AV +GG+PF++A+ G+ AFEY   DPRFN+VFN  M NH+T+++KKI+
Sbjct: 132 KVLMESWYHLSDAVVDGGIPFNKAY-GMTAFEYHGTDPRFNKVFNQGMSNHSTITMKKIL 191

Query: 190 ESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDM 249
           E+Y GF  +K +VDVGGG G TL +I S YPSIKGINFDLPHVI DAP+Y GV+HVGGDM
Sbjct: 192 ETYTGFDGLKTVVDVGGGTGATLNMIVSKYPSIKGINFDLPHVIEDAPSYPGVEHVGGDM 251

Query: 250 FESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNVTKA 309
           F SVP GDAIFMKWI HDWSD+HC K LKNCY+A+P +GK+I+ E V+P  P+    TK 
Sbjct: 252 FVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYDALPQNGKVILAECVLPEAPDTGLATKN 311

Query: 310 TTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
               DV+M+  NPGGKERT  EF+ LA  AGFK     C   N W++ELLK
Sbjct: 312 VVHIDVIMLAHNPGGKERTEKEFQGLAKAAGFKQFNKACCAYNTWIMELLK 361

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BLAST of CSPI03G04000 vs. ExPASy Swiss-Prot
Match: P28002 (Caffeic acid 3-O-methyltransferase OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 6.3e-114
Identity = 190/353 (53.82%), Postives = 257/353 (72.80%), Query Frame = 0


Query: 8   TAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNP 67
           T    ++   +A  L + SVLPM L++  EL + EI+AKAG GA +SP EIA+++ TTNP
Sbjct: 12  THISDEEANLFAMQLASASVLPMILKSALELDLLEIIAKAGPGAQISPIEIASQLPTTNP 71

Query: 68  NAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLI 127
           +A +MLDRMLRLLAC+ ++ CS+ + +DG VQRLY L  ++K  V+NEDGVS+  +  + 
Sbjct: 72  DAPVMLDRMLRLLACYIILTCSVRTQQDGKVQRLYGLATVAKYLVKNEDGVSISALNLMN 131

Query: 128 QDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKK 187
           QDKV ++SW  LK+AV +GG+PF++A+ G+ AFEY   DPRFN+VFN  M +H+T+++KK
Sbjct: 132 QDKVLMESWYHLKDAVLDGGIPFNKAY-GMTAFEYHGTDPRFNKVFNKGMSDHSTITMKK 191

Query: 188 IVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGG 247
           I+E+Y GF  +K LVDVGGG G  +  I S YP+IKGINFDLPHVI DAP+Y GV+HVGG
Sbjct: 192 ILETYTGFEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGG 251

Query: 248 DMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNVT 307
           DMF S+P  DA+FMKWI HDWSD+HC K LKNCY A+PD+GK+I+ E ++P  P+ +  T
Sbjct: 252 DMFVSIPKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLAT 311

Query: 308 KATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
           K     DV+M+  NPGGKERT+ EF+ LA  AGF+     C   N +++E LK
Sbjct: 312 KGVVHIDVIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIMEFLK 363

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BLAST of CSPI03G04000 vs. ExPASy Swiss-Prot
Match: Q8W013 (Caffeic acid 3-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=COMT1 PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 1.8e-113
Identity = 194/355 (54.65%), Postives = 259/355 (72.96%), Query Frame = 0


Query: 6   NDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTT 65
           N    E+++    A  L + SVLPM L++  EL + E++ K+G GA +SP+E+AA++ T 
Sbjct: 10  NSMTKEEEEACLSAMRLASASVLPMVLKSAIELDLLELIKKSGPGAYVSPSELAAQLPTQ 69

Query: 66  NPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLS 125
           NP+A +MLDR+LRLLA +SV+ C++    DG ++RLYSL P+ K   +NEDGVS+  +L 
Sbjct: 70  NPDAPVMLDRILRLLASYSVLNCTLKDLPDGGIERLYSLAPVCKFLTKNEDGVSMAALLL 129

Query: 126 LIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSI 185
           + QDKV ++SW  LK+AV EGG+PF++A+ G+ AFEY   DPRFN+VFN  M NH+T+ +
Sbjct: 130 MNQDKVLMESWYHLKDAVLEGGIPFNKAY-GMTAFEYHGKDPRFNKVFNQGMSNHSTIIM 189

Query: 186 KKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHV 245
           KKI+E Y+GF  +K +VDVGGG G TL +I S YPSIKGINFDLPHVI DAP+Y GV HV
Sbjct: 190 KKILEIYQGFQGLKTVVDVGGGTGATLNMIVSKYPSIKGINFDLPHVIEDAPSYPGVDHV 249

Query: 246 GGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITN 305
           GGDMF SVP GDAIFMKWI HDWSD HC K LKNC+ A+P++GK+I+ E ++P  P+ T 
Sbjct: 250 GGDMFVSVPKGDAIFMKWICHDWSDAHCLKFLKNCHEALPENGKVILAECLLPEAPDSTL 309

Query: 306 VTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
            T+ T   DV+M+  NPGGKERT  EF++LA  AGF+  +  C   N W++ELLK
Sbjct: 310 STQNTVHVDVIMLAHNPGGKERTEKEFEALAKGAGFRGFIKVCCAYNSWIMELLK 363

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BLAST of CSPI03G04000 vs. ExPASy Swiss-Prot
Match: O81646 (Caffeic acid 3-O-methyltransferase OS=Capsicum chinense OX=80379 GN=COMT PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 4.1e-113
Identity = 193/360 (53.61%), Postives = 255/360 (70.83%), Query Frame = 0


Query: 1   MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           M S +      + + + +A  L + SVLPM L+A  EL + EI+AK+G GA +SP+E+AA
Sbjct: 1   MGSINQSLTQTEDEAFVFAMQLASASVLPMVLKATVELDLLEIMAKSGPGAFISPSELAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           ++ T NP A +MLDRM RLLA +SV+ C++ +  DG V+RLYSL P+ K   +N DGVS+
Sbjct: 61  QLPTKNPEAPVMLDRMFRLLATYSVLNCTLRTLPDGRVERLYSLAPVCKFLTKNGDGVSI 120

Query: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180
            P+L + QDKV ++SW  L +AV +GGVPF++A+ G+  FEY   DPRFN+VFN  M +H
Sbjct: 121 APILLMNQDKVLMESWYHLTDAVLDGGVPFNKAY-GMTTFEYHGTDPRFNKVFNCGMSDH 180

Query: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN 240
           TT+S+KKI+E Y GF  +  +VDVGGG G T+ +I S YPSIKGINFDLPHVIRDAP+Y 
Sbjct: 181 TTLSMKKILEDYTGFEGLNSIVDVGGGTGATVNMIVSKYPSIKGINFDLPHVIRDAPSYP 240

Query: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK 300
           GV+ VGGDMF SVP  DAIFMKWI HDWSDDHC KLLKNCY A+P +GK+IIVE ++P  
Sbjct: 241 GVEQVGGDMFVSVPKADAIFMKWICHDWSDDHCIKLLKNCYEALPANGKVIIVECILPEA 300

Query: 301 PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 360
           P+ +  TK+   GD++M+  NPGGKERT  +F++LA    F      C   + WV+E  K
Sbjct: 301 PDTSAATKSKVHGDIIMLAHNPGGKERTEKDFEALANWGWFSRFRKVCCAYHTWVMEFNK 359

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BLAST of CSPI03G04000 vs. ExPASy TrEMBL
Match: A0A1S3BM08 (caffeic acid 3-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103491014 PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 8.9e-180
Identity = 315/361 (87.26%), Postives = 335/361 (92.80%), Query Frame = 0


Query: 1   MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDNDTA E QQ+YA A  LVTLSVLPMTLQAVFELGVFEILAKAGD   LSPAEI A
Sbjct: 1   MASSDNDTAVE-QQNYANAAELVTLSVLPMTLQAVFELGVFEILAKAGD-RELSPAEITA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP AA M+DRMLRLLA HSVVGCSI SD+DGNVQR YSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPYAASMIDRMLRLLASHSVVGCSIASDEDGNVQRFYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180
           GP+LSLIQDKVFLQSWSELKNAV EGGVPFDRAHGGV+AFEYP LDPRFNQVFNI M+NH
Sbjct: 121 GPILSLIQDKVFLQSWSELKNAVIEGGVPFDRAHGGVHAFEYPSLDPRFNQVFNIGMINH 180

Query: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN 240
           TTMSIK+IV+SYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAP+Y+
Sbjct: 181 TTMSIKEIVKSYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYH 240

Query: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK 300
           GV+HVGGDMFE+VPNGDAIFMKWILHDWSDDHC KLLKNCYNAIPDDGK+IIVE V PT+
Sbjct: 241 GVEHVGGDMFENVPNGDAIFMKWILHDWSDDHCLKLLKNCYNAIPDDGKVIIVECVFPTE 300

Query: 301 PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 360
           PE T VTK+  QGDVLMMTQNPGGKERTRDEFK+LATKAGFKH++F+CFV+NLWVIE LK
Sbjct: 301 PETTTVTKSIAQGDVLMMTQNPGGKERTRDEFKTLATKAGFKHLIFQCFVSNLWVIEFLK 359

Query: 361 N 362
           N
Sbjct: 361 N 359

[Close]

BLAST of CSPI03G04000 vs. ExPASy TrEMBL
Match: A0A6J1CQX8 (caffeic acid 3-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111013400 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.6e-160
Identity = 275/350 (78.57%), Postives = 305/350 (87.14%), Query Frame = 0


Query: 11  EQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNAA 70
           ++QQH+AYA HLVTLSV P TLQA FELGVFEIL KAG GA LS AEIAA+IT TNPNAA
Sbjct: 14  QEQQHFAYAAHLVTLSVFPFTLQAAFELGVFEILTKAGPGAELSAAEIAAKITATNPNAA 73

Query: 71  LMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLIQDK 130
            M+DR+LRLLA HSVVGCS   D+ GNV+RLYSLTP+SK +VRNEDGVSLGP++ LIQDK
Sbjct: 74  SMVDRILRLLATHSVVGCSTAEDEGGNVKRLYSLTPVSKYFVRNEDGVSLGPVMELIQDK 133

Query: 131 VFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIVE 190
           VFL SW ELKNAV EGGV F+RAHGGV+AFEYP LDPRFN+VFN AM+NHTTM IKKIVE
Sbjct: 134 VFLDSWRELKNAVIEGGVAFNRAHGGVHAFEYPGLDPRFNKVFNTAMINHTTMGIKKIVE 193

Query: 191 SYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDMF 250
           SYKGF N+ QLVDVGGGLGVTLQ+ITS YPSIKGINFDLPHVIRDAPAY GV+H+GGDMF
Sbjct: 194 SYKGFGNLNQLVDVGGGLGVTLQVITSKYPSIKGINFDLPHVIRDAPAYPGVEHIGGDMF 253

Query: 251 ESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNVTKAT 310
           ESVP GDAIFMKWILHDWSD+HC KLLKNCY AIP+DGK+I V++V+P   E T   K+ 
Sbjct: 254 ESVPKGDAIFMKWILHDWSDEHCVKLLKNCYAAIPNDGKVIAVDAVVPLVIETTITMKSI 313

Query: 311 TQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
           TQGDVLMMTQNPGGKERTRDEFK+LATK GFK + FECFV NLWVIE +K
Sbjct: 314 TQGDVLMMTQNPGGKERTRDEFKALATKTGFKRINFECFVYNLWVIEFIK 363

[Close]

BLAST of CSPI03G04000 vs. ExPASy TrEMBL
Match: A0A6J1FG58 (caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443875 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 7.9e-160
Identity = 267/360 (74.17%), Postives = 320/360 (88.89%), Query Frame = 0


Query: 1   MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASS++D  +++QQHY+YA  LVTLSVLPMTLQA FELG+FEILAKAGDGA LSP EIAA
Sbjct: 11  MASSNSDD-SQEQQHYSYAAQLVTLSVLPMTLQAAFELGLFEILAKAGDGAELSPTEIAA 70

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           +ITT+NP+AA M+DR+LRL+A HSVVGCS++ D++GN QRLYSL P++K ++ NEDGVSL
Sbjct: 71  KITTSNPDAASMIDRILRLMAAHSVVGCSVIIDENGNQQRLYSLMPVTKYFIPNEDGVSL 130

Query: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180
           GP++S+IQDKVFL SWSELKNAV EGGVPF+RAHGGV+AFEYP+LD RFNQ+FN AM+N+
Sbjct: 131 GPIVSMIQDKVFLHSWSELKNAVIEGGVPFNRAHGGVHAFEYPRLDQRFNQIFNNAMINY 190

Query: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN 240
           +T +IK +V+ Y GFAN+KQLVDVGGGLGVTL+IITS YPSIKGINFDLPHVI+DAP+Y 
Sbjct: 191 STTAIKAVVKCYNGFANLKQLVDVGGGLGVTLKIITSAYPSIKGINFDLPHVIQDAPSYP 250

Query: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK 300
           GV+HVGGDMFE VP GDAIF+KWILHDWSD+HC KLLKNCY+AIPDDGK+I++E V+PT 
Sbjct: 251 GVEHVGGDMFEKVPEGDAIFIKWILHDWSDEHCVKLLKNCYSAIPDDGKVIVMEGVVPTV 310

Query: 301 PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 360
           PE T   KA +QGD+LMMTQNPGGKER+RDEFK+LATKAGFKH+   CF+ N W+IE LK
Sbjct: 311 PETTAAEKAISQGDLLMMTQNPGGKERSRDEFKALATKAGFKHIKLVCFLCNFWIIEFLK 369

[Close]

BLAST of CSPI03G04000 vs. ExPASy TrEMBL
Match: A0A6J1FGP5 (caffeic acid 3-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111443858 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 1.1e-158
Identity = 266/360 (73.89%), Postives = 320/360 (88.89%), Query Frame = 0


Query: 1   MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASS++D  +++QQHY+YA  LVTLSVLPMTLQA FELG+FEILAKAGDGA LSP EIAA
Sbjct: 11  MASSNSDD-SQEQQHYSYAAQLVTLSVLPMTLQAAFELGLFEILAKAGDGAELSPTEIAA 70

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           +ITT+NP+AA M+DR+LRL+A HSVVGCS++ D++GN QRLYSL P++K ++ NEDGVSL
Sbjct: 71  KITTSNPDAASMIDRILRLMAAHSVVGCSVIIDENGNQQRLYSLMPVTKYFIPNEDGVSL 130

Query: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180
           GP++S+IQDKVFL SWSELKNAV EGGVPF+RAHGGV+AFEYP+LD RFNQ+FN AM+N+
Sbjct: 131 GPIVSMIQDKVFLHSWSELKNAVIEGGVPFNRAHGGVHAFEYPRLDQRFNQIFNNAMINY 190

Query: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN 240
           +T +IK +V+ Y GFAN+KQLVDVGGGLGVTL+IITS YPSIKGINFDLPHVI+DAP+Y 
Sbjct: 191 STTAIKAVVKCYNGFANLKQLVDVGGGLGVTLKIITSAYPSIKGINFDLPHVIQDAPSYP 250

Query: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK 300
           GV+HVGGDMFE VP GDAIF+KWILHDWSD+HC KLLKNCY AIP+DGK+I++E V+PT 
Sbjct: 251 GVEHVGGDMFEKVPEGDAIFIKWILHDWSDEHCVKLLKNCYAAIPNDGKVIVMEGVLPTV 310

Query: 301 PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 360
           PE ++  K   QGDVLMMTQNPGGKERTRDEF++LATKAGFKHV + CF++N W+IE LK
Sbjct: 311 PETSDAAKGIIQGDVLMMTQNPGGKERTRDEFEALATKAGFKHVKYVCFLSNHWIIEFLK 369

[Close]

BLAST of CSPI03G04000 vs. ExPASy TrEMBL
Match: A0A6J1L279 (caffeic acid 3-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498431 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.5e-158
Identity = 266/355 (74.93%), Postives = 314/355 (88.45%), Query Frame = 0


Query: 6   NDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTT 65
           N    ++QQHY+YA  LVT+SVLPMTLQA FELG+FEILAKAGDGA LSP EIAA+ITT+
Sbjct: 15  NSDDGQEQQHYSYAAQLVTISVLPMTLQAAFELGLFEILAKAGDGAELSPTEIAAKITTS 74

Query: 66  NPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLS 125
           NP+AA M+DR+LRLLA HSVVGCS++ D++GN QRLYSL P++K +V NEDGVSLGP++S
Sbjct: 75  NPDAASMIDRILRLLAAHSVVGCSVIIDENGNQQRLYSLKPVAKYFVPNEDGVSLGPIIS 134

Query: 126 LIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSI 185
           +IQDKVFL SWSELKNAV EGGVPF+RAHGGV+AFEYP+LD RFNQVFN AM+N++T +I
Sbjct: 135 MIQDKVFLHSWSELKNAVIEGGVPFNRAHGGVHAFEYPRLDQRFNQVFNNAMINYSTTAI 194

Query: 186 KKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHV 245
           K +V+ Y GFA++KQLVDVGGGLGVTL+IITS YPSIKGINFDLPHVI+DAP+Y GV+HV
Sbjct: 195 KAVVKCYNGFASLKQLVDVGGGLGVTLKIITSAYPSIKGINFDLPHVIQDAPSYPGVEHV 254

Query: 246 GGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITN 305
           GGDMFE VP GDAIF+KWILHDWSD+HC KLLKNCY AIPDDGK+I++E V+PT PE ++
Sbjct: 255 GGDMFEKVPEGDAIFIKWILHDWSDEHCEKLLKNCYAAIPDDGKVIVMEGVLPTVPETSD 314

Query: 306 VTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
           V K   QGDVLMMTQNPGGKERTRDEF++LATKAGFKHV F CF++N W+IE LK
Sbjct: 315 VAKGIIQGDVLMMTQNPGGKERTRDEFEALATKAGFKHVKFVCFLSNHWIIEFLK 369

[Close]

BLAST of CSPI03G04000 vs. NCBI nr
Match: XP_004150935.3 (caffeic acid 3-O-methyltransferase [Cucumis sativus] >KAE8650102.1 hypothetical protein Csa_010461 [Cucumis sativus])

HSP 1 Score: 732.3 bits (1889), Expect = 2.1e-207
Identity = 360/361 (99.72%), Postives = 360/361 (99.72%), Query Frame = 0


Query: 1   MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDNDTA EQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA
Sbjct: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL
Sbjct: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180
           GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH
Sbjct: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180

Query: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN 240
           TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN
Sbjct: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN 240

Query: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK 300
           GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK
Sbjct: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK 300

Query: 301 PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 360
           PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK
Sbjct: 301 PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 360

Query: 361 N 362
           N
Sbjct: 361 N 361

[Close]

BLAST of CSPI03G04000 vs. NCBI nr
Match: KAE8650103.1 (hypothetical protein Csa_010827 [Cucumis sativus])

HSP 1 Score: 669.1 bits (1725), Expect = 2.2e-188
Identity = 334/372 (89.78%), Postives = 345/372 (92.74%), Query Frame = 0


Query: 1   MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDN+TA EQQQ+YAYA  LVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA
Sbjct: 1   MASSDNNTAVEQQQNYAYAAQLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP+AALMLDRMLRLLACHSVVGCSI SDKDGNVQRLYSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPDAALMLDRMLRLLACHSVVGCSIASDKDGNVQRLYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180
           GP LSLIQDKVFLQSWSELKNAV EGGVPFDRAHGGV+AFEYPKLDPRFNQVFNIAM+NH
Sbjct: 121 GPTLSLIQDKVFLQSWSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINH 180

Query: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDA---- 240
           TT+SIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDA    
Sbjct: 181 TTVSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDALLIM 240

Query: 241 -------PAYNGVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGK 300
                      GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGK
Sbjct: 241 FTKLNFDYELAGVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGK 300

Query: 301 IIIVESVIPTKPEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECF 360
           IIIV+SVIP +PE TNVTKAT Q DVLMMTQNPGGKERTRDEFKSLATKAGFKHV+F+CF
Sbjct: 301 IIIVDSVIPMEPETTNVTKATAQADVLMMTQNPGGKERTRDEFKSLATKAGFKHVIFQCF 360

Query: 361 VANLWVIELLKN 362
           V+NLWVIE LKN
Sbjct: 361 VSNLWVIEFLKN 372

[Close]

BLAST of CSPI03G04000 vs. NCBI nr
Match: XP_008449018.1 (PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 639.4 bits (1648), Expect = 1.8e-179
Identity = 315/361 (87.26%), Postives = 335/361 (92.80%), Query Frame = 0


Query: 1   MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDNDTA E QQ+YA A  LVTLSVLPMTLQAVFELGVFEILAKAGD   LSPAEI A
Sbjct: 1   MASSDNDTAVE-QQNYANAAELVTLSVLPMTLQAVFELGVFEILAKAGD-RELSPAEITA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP AA M+DRMLRLLA HSVVGCSI SD+DGNVQR YSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPYAASMIDRMLRLLASHSVVGCSIASDEDGNVQRFYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180
           GP+LSLIQDKVFLQSWSELKNAV EGGVPFDRAHGGV+AFEYP LDPRFNQVFNI M+NH
Sbjct: 121 GPILSLIQDKVFLQSWSELKNAVIEGGVPFDRAHGGVHAFEYPSLDPRFNQVFNIGMINH 180

Query: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN 240
           TTMSIK+IV+SYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAP+Y+
Sbjct: 181 TTMSIKEIVKSYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYH 240

Query: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK 300
           GV+HVGGDMFE+VPNGDAIFMKWILHDWSDDHC KLLKNCYNAIPDDGK+IIVE V PT+
Sbjct: 241 GVEHVGGDMFENVPNGDAIFMKWILHDWSDDHCLKLLKNCYNAIPDDGKVIIVECVFPTE 300

Query: 301 PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 360
           PE T VTK+  QGDVLMMTQNPGGKERTRDEFK+LATKAGFKH++F+CFV+NLWVIE LK
Sbjct: 301 PETTTVTKSIAQGDVLMMTQNPGGKERTRDEFKTLATKAGFKHLIFQCFVSNLWVIEFLK 359

Query: 361 N 362
           N
Sbjct: 361 N 359

[Close]

BLAST of CSPI03G04000 vs. NCBI nr
Match: XP_031738579.1 (LOW QUALITY PROTEIN: caffeic acid 3-O-methyltransferase [Cucumis sativus])

HSP 1 Score: 636.7 bits (1641), Expect = 1.2e-178
Identity = 313/360 (86.94%), Postives = 331/360 (91.94%), Query Frame = 0


Query: 1   MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDN+TA EQQQ+YAYA  LVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA
Sbjct: 1   MASSDNNTAVEQQQNYAYAAQLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP+AALMLDRMLRLLACHSVVGCSI SDKDGNVQRLYSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPDAALMLDRMLRLLACHSVVGCSIASDKDGNVQRLYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180
           GP LSLIQDKVFLQSWSELKNAV EGGVPFDRAHGGV+AFEYPKLDPRFNQVFNIAM+NH
Sbjct: 121 GPTLSLIQDKVFLQSWSELKNAVIEGGVPFDRAHGGVHAFEYPKLDPRFNQVFNIAMINH 180

Query: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN 240
           TT+SIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRD PAYN
Sbjct: 181 TTVSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDXPAYN 240

Query: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK 300
           GVQHVGGDMFESVPNGDAIFMKWILHDW+DD C KLLKNCY+AIP+DGK+I+V++V    
Sbjct: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWNDDKCVKLLKNCYDAIPNDGKVIVVDAVHTMV 300

Query: 301 PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 360
           PE T   +   QGDV MMTQN GGKER+RDEFK+LATKAGF+H+ F   V NLWVIEL K
Sbjct: 301 PETTCAARVVAQGDVFMMTQNRGGKERSRDEFKALATKAGFEHINFHSCVYNLWVIELFK 360

[Close]

BLAST of CSPI03G04000 vs. NCBI nr
Match: XP_038903769.1 (caffeic acid 3-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 609.4 bits (1570), Expect = 2.0e-170
Identity = 294/361 (81.44%), Postives = 325/361 (90.03%), Query Frame = 0


Query: 1   MASSDNDTAAEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           + SSDND   E QQHYAYA  LVTLSVLPMTLQAVFELGVFEILAKAGDGA LSPA+IAA
Sbjct: 52  ITSSDNDAVLE-QQHYAYAAQLVTLSVLPMTLQAVFELGVFEILAKAGDGAELSPADIAA 111

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITT NP+AALM+DRML LLA HSVVG S+  D+DG  QRLYSLTP+SK YVRNEDGVSL
Sbjct: 112 EITTKNPDAALMIDRMLWLLASHSVVGFSVGFDEDGKTQRLYSLTPVSKYYVRNEDGVSL 171

Query: 121 GPMLSLIQDKVFLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNH 180
           GP LSLIQDKVFL SWSELK+AV EGGVPF+RAHGGV+AFEYP LDPRFNQVFN  M+NH
Sbjct: 172 GPTLSLIQDKVFLHSWSELKHAVIEGGVPFNRAHGGVHAFEYPGLDPRFNQVFNTGMLNH 231

Query: 181 TTMSIKKIVESYKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYN 240
           TTM IKK+V++Y GFANI+QLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAP+Y 
Sbjct: 232 TTMCIKKLVKTYNGFANIEQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYP 291

Query: 241 GVQHVGGDMFESVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTK 300
           GV+HVGGDMF+ VPNGD +FMKWILHDWSDDHC KLLKNCY AIP+DGK+I+V+SV+PT 
Sbjct: 292 GVEHVGGDMFQKVPNGDVVFMKWILHDWSDDHCIKLLKNCYVAIPNDGKVIVVDSVVPTI 351

Query: 301 PEITNVTKATTQGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 360
           PE TNVTK+  QGDVLMMTQNPGGKERTRDEFK++A KAGFKH++F+CFV+NLWVIE LK
Sbjct: 352 PETTNVTKSIAQGDVLMMTQNPGGKERTRDEFKAIAIKAGFKHIIFQCFVSNLWVIEFLK 411

Query: 361 N 362
           N
Sbjct: 412 N 411

[Close]

BLAST of CSPI03G04000 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 387.5 bits (994), Expect = 1.2e-107
Identity = 181/343 (52.77%), Postives = 250/343 (72.89%), Query Frame = 0


Query: 18  YAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNAALMLDRML 77
           +A  L + SVLPM L++  EL + EI+AK  +G+ +SP EIA+++ T NP A +MLDR+L
Sbjct: 22  FAMQLASASVLPMALKSALELDLLEIMAK--NGSPMSPTEIASKLPTKNPEAPVMLDRIL 81

Query: 78  RLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLIQDKVFLQSWS 137
           RLL  +SV+ CS        V+R+Y L P+ K   +NEDGVS+  +  + QDKV ++SW 
Sbjct: 82  RLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWY 141

Query: 138 ELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIVESYKGFAN 197
            LK+A+ +GG+PF++A+ G++AFEY   DPRFN+VFN  M NH+T+++KKI+E+YKGF  
Sbjct: 142 HLKDAILDGGIPFNKAY-GMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFEG 201

Query: 198 IKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDMFESVPNGD 257
           +  LVDVGGG+G TL++I S YP++KGINFDLPHVI DAP++ G++HVGGDMF SVP GD
Sbjct: 202 LTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGD 261

Query: 258 AIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNVTKATTQGDVLM 317
           AIFMKWI HDWSD+HC K LKNCY ++P+DGK+I+ E ++P  P+ +  TK     D +M
Sbjct: 262 AIFMKWICHDWSDEHCVKFLKNCYESLPEDGKVILAECILPETPDSSLSTKQVVHVDCIM 321

Query: 318 MTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
           +  NPGGKERT  EF++LA  +GFK +   C    + +IELLK
Sbjct: 322 LAHNPGGKERTEKEFEALAKASGFKGIKVVCDAFGVNLIELLK 361

[Close]

BLAST of CSPI03G04000 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.3e-85
Identity = 159/350 (45.43%), Postives = 226/350 (64.57%), Query Frame = 0


Query: 19  AGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITT--TNPNAALMLDRM 78
           A  +V     PM L+A FELGV + +A AG+   LSP EIA  + T  TNP A ++LDRM
Sbjct: 33  AESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRM 92

Query: 79  LRLLACHSVVGCSIV----SDKDGNVQRLYSLTPISKCYVRNEDGV-SLGPMLSLIQDKV 138
           L LL  HS++ C ++    + + G ++R+Y+  P+ K ++R+ DG  SL P+  L+  +V
Sbjct: 93  LSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQV 152

Query: 139 FLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIVES 198
           F ++W+ LK+ + EG   F+ AH G+  FEY   D  F ++FN AM   +TM +KK+++ 
Sbjct: 153 FFKTWTNLKDVILEGRDAFNSAH-GMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVLDV 212

Query: 199 YKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDMFE 258
           Y+GF ++  LVDVGGG G  L ++TS YP IKG+NFDL  V+  AP Y GV+HV GDMF 
Sbjct: 213 YRGFEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFV 272

Query: 259 SVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNVTKATT 318
            VP GDA+FMKWILHDW D+ C K+LKNC+ ++P+ GKIIIVE V P +P+  +++  T 
Sbjct: 273 EVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEKGKIIIVEFVTPKEPKGGDLSSNTV 332

Query: 319 QG-DVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
              D+LM+TQ  GGKER+  +F++LA  +GF      C   +  VIE  K
Sbjct: 333 FAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIEFHK 381

[Close]

BLAST of CSPI03G04000 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 312.4 bits (799), Expect = 4.8e-85
Identity = 159/350 (45.43%), Postives = 227/350 (64.86%), Query Frame = 0


Query: 19  AGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITT--TNPNAALMLDRM 78
           A  +V     PM L+A  ELGV + +A A +G  LSP+EIA  +    TNP A ++LDRM
Sbjct: 33  AESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPEAPVLLDRM 92

Query: 79  LRLLACHSVVGCSIVSDKD----GNVQRLYSLTPISKCYVRNEDGV-SLGPMLSLIQDKV 138
           LRLL  HS++ C +V  ++    G ++R+Y+  PI K ++++ DG  SL  +L L+  +V
Sbjct: 93  LRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSLLLLLHSQV 152

Query: 139 FLQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIVES 198
            L++W+ LK+ + EG   F  AH  +  FEY   D +F+++F+ AM   +TM +KK++E 
Sbjct: 153 ILKTWTNLKDVILEGKDAFSSAH-DMRLFEYISSDDQFSKLFHRAMSESSTMVMKKVLEE 212

Query: 199 YKGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDMFE 258
           Y+GF ++  LVDVGGG+G  L +ITS YP IKG+NFDL  V+  AP Y GV+HV GDMF 
Sbjct: 213 YRGFEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQAPFYPGVKHVSGDMFI 272

Query: 259 SVPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNVTKATT 318
            VP GDAIFMKWILHDW D+ C K+LKNC+ ++P+ GK+IIVE + P +P+  + +  T 
Sbjct: 273 EVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEKGKVIIVEMITPMEPKPNDFSCNTV 332

Query: 319 QG-DVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
            G D+LM+TQ  GGKER+  +F++LA  +GF      C   +  VIE  K
Sbjct: 333 LGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYSVIEFHK 381

[Close]

BLAST of CSPI03G04000 vs. TAIR 10
Match: AT1G21100.1 (O-methyltransferase family protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.3e-82
Identity = 158/349 (45.27%), Postives = 223/349 (63.90%), Query Frame = 0


Query: 19  AGHLVTLSVLPMTLQAVFELGVFEILAKAGDGAN--LSPAEIAAEITTT--NPNAALMLD 78
           A  L   +  PM L+A  ELGVF+ L  A    +  LSP EIA+++ TT  NP A ++LD
Sbjct: 29  AVRLANAAAFPMVLKAALELGVFDTLYAAASRTDSFLSPYEIASKLPTTPRNPEAPVLLD 88

Query: 79  RMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRN--EDGVSLGPMLSLIQDKVF 138
           RMLRLLA +S+V C       G  +R+Y   PI + ++++  +D  SL   + +  D VF
Sbjct: 89  RMLRLLASYSMVKCG--KALSGKGERVYRAEPICRFFLKDNIQDIGSLASQVIVNFDSVF 148

Query: 139 LQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIVESY 198
           L +W++LK+ V EGG  F RAHGG+  F+Y   D RF+++FN      T   +KK +E Y
Sbjct: 149 LNTWAQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFN--QTGFTIAVVKKALEVY 208

Query: 199 KGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDMFES 258
           +GF  +K LVDVGGG+G TL ++TS YP+IKGINFDL   +  AP+Y GV+HV GDMF  
Sbjct: 209 EGFKGVKVLVDVGGGVGNTLGVVTSKYPNIKGINFDLTCALAQAPSYPGVEHVAGDMFVD 268

Query: 259 VPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNV-TKATT 318
           VP GDA+ +K ILHDW+D+ C K+LKNC+ ++P++GK++++E V P + E  ++      
Sbjct: 269 VPTGDAMILKRILHDWTDEDCVKILKNCWKSLPENGKVVVIELVTPDEAENGDINANIAF 328

Query: 319 QGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
             D+LM TQ  GGKER+R EF++LA  +GF H  F C   + W+IE  K
Sbjct: 329 DMDMLMFTQCSGGKERSRAEFEALAAASGFTHCKFVCQAYHCWIIEFCK 373

[Close]

BLAST of CSPI03G04000 vs. TAIR 10
Match: AT1G21130.1 (O-methyltransferase family protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.3e-82
Identity = 158/349 (45.27%), Postives = 224/349 (64.18%), Query Frame = 0


Query: 19  AGHLVTLSVLPMTLQAVFELGVFEILAKAGDGAN--LSPAEIAAEITTT--NPNAALMLD 78
           A  L   +  PM L+A  ELGVF+ L      ++  LSP+EIA+++ TT  NP A ++LD
Sbjct: 29  AVRLANAAAFPMVLKAALELGVFDTLYAEASRSDSFLSPSEIASKLPTTPRNPEAPVLLD 88

Query: 79  RMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRN--EDGVSLGPMLSLIQDKVF 138
           RMLRLLA +SVV C  VS  +G  +R+Y   PI + ++++  +D  SL   + +  D VF
Sbjct: 89  RMLRLLASYSVVKCGKVS--EGKGERVYRAEPICRFFLKDNIQDIGSLASQVIVNFDSVF 148

Query: 139 LQSWSELKNAVTEGGVPFDRAHGGVNAFEYPKLDPRFNQVFNIAMVNHTTMSIKKIVESY 198
           L +W++LK+ V EGG  F RAHGG+  F+Y   D RF+++FN      T   +KK +E Y
Sbjct: 149 LNTWAQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFN--QTGFTIAVVKKALEVY 208

Query: 199 KGFANIKQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPAYNGVQHVGGDMFES 258
           +GF  +  LVDVGGG+G TL ++ S YP+IKGINFDL   +  AP+Y GV+HV GDMF  
Sbjct: 209 QGFKGVNVLVDVGGGVGNTLGVVASKYPNIKGINFDLTCALAQAPSYPGVEHVAGDMFVD 268

Query: 259 VPNGDAIFMKWILHDWSDDHCTKLLKNCYNAIPDDGKIIIVESVIPTKPEITNV-TKATT 318
           VP GDA+ +K ILHDW+D+ C K+LKNC+ ++P+ GK++++E V P + E  ++      
Sbjct: 269 VPTGDAMILKRILHDWTDEDCVKILKNCWKSLPESGKVVVIELVTPDEAENGDINANIAF 328

Query: 319 QGDVLMMTQNPGGKERTRDEFKSLATKAGFKHVMFECFVANLWVIELLK 361
             D+LM TQ  GGKER+R EF++LA  +GF H  F C   + W+IE  K
Sbjct: 329 DMDMLMFTQCSGGKERSRAEFEALAAASGFTHCKFVCQAYHCWIIEFCK 373

The following BLAST results are available for this feature:

BLAST of CSPI03G04000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LL87	9.7e-115	55.01	Caffeic acid 3-O-methyltransferase OS=Coffea canephora OX=49390 GN=COMT PE=2 SV=...	[more]
Q9XGW0	4.8e-114	53.85	Caffeic acid 3-O-methyltransferase 1 OS=Ocimum basilicum OX=39350 GN=COMT1 PE=2 ...	[more]
P28002	6.3e-114	53.82	Caffeic acid 3-O-methyltransferase OS=Medicago sativa OX=3879 PE=1 SV=1	[more]
Q8W013	1.8e-113	54.65	Caffeic acid 3-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=COMT1 PE=2 ...	[more]
O81646	4.1e-113	53.61	Caffeic acid 3-O-methyltransferase OS=Capsicum chinense OX=80379 GN=COMT PE=2 SV...	[more]

BLAST of CSPI03G04000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BM08	8.9e-180	87.26	caffeic acid 3-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103491014 ...	[more]
A0A6J1CQX8	1.6e-160	78.57	caffeic acid 3-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1FG58	7.9e-160	74.17	caffeic acid 3-O-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1FGP5	1.1e-158	73.89	caffeic acid 3-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1L279	1.5e-158	74.93	caffeic acid 3-O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of CSPI03G04000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150935.3	2.1e-207	99.72	caffeic acid 3-O-methyltransferase [Cucumis sativus] >KAE8650102.1 hypothetical ...	[more]
KAE8650103.1	2.2e-188	89.78	hypothetical protein Csa_010827 [Cucumis sativus]	[more]
XP_008449018.1	1.8e-179	87.26	PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis melo]	[more]
XP_031738579.1	1.2e-178	86.94	LOW QUALITY PROTEIN: caffeic acid 3-O-methyltransferase [Cucumis sativus]	[more]
XP_038903769.1	2.0e-170	81.44	caffeic acid 3-O-methyltransferase-like [Benincasa hispida]	[more]

BLAST of CSPI03G04000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54160.1	1.2e-107	52.77	O-methyltransferase 1	[more]
AT1G77520.1	1.3e-85	45.43	O-methyltransferase family protein	[more]
AT1G77530.1	4.8e-85	45.43	O-methyltransferase family protein	[more]
AT1G21100.1	1.3e-82	45.27	O-methyltransferase family protein	[more]
AT1G21130.1	1.3e-82	45.27	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 1..360 e-value: 1.6E-131 score: 436.4
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 14..359
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 20..360 score: 69.327576
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 2..110 e-value: 6.1E-39 score: 134.1
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 112..360 e-value: 4.2E-90 score: 303.0
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 29..358 e-value: 7.9E-93 score: 308.5
None	No IPR available	PIRSR	PIRSR016958-1	PIRSR016958-1	coord: 183..296 e-value: 6.3E-5 score: 20.4
None	No IPR available	PANTHER	PTHR11746:SF220	CAFFEIC ACID 3-O-METHYLTRANSFERASE 1-LIKE	coord: 14..359
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 30..81 e-value: 1.1E-17 score: 63.7
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 136..342 e-value: 8.8E-67 score: 224.5
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 11..114
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 117..357

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G04000.1	CSPI03G04000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity