CSPI03G03930 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G03930
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	subtilisin-like protease SBT5.3
Location	Chr3: 3216755 .. 3219274 (-)
RNA-Seq Expression	CSPI03G03930
Synteny	CSPI03G03930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTTCCGAAAAACAAACGGCCCCTAACACTGTTCATGTAATTGGGATTATAGGTGTTTGGCCAGAATCTAAGAGCTTTAGTGATGAAGGCTATGGACCTATCCCCCCAAGGTGGATGGGAACTTGTCAAAGTGATGCCGACCCCAAGTTTCGTTGTAATAGGTCAAGAGATTCATATCCTACATCAGTTTTTTTTCTTCTATTTCTATTATGGTAGTTAATTCAAAATGGCTACCTTAAATGCAGAAAATTGATTGGAGCAAGATTCTTCAACATAGGCTATGGTGAACTTACTGATACCTTCAATTCTTCAAGAGACAATGTAGGGCATGGAACCCACACTTTATCCATAGCTGGTGGCAATTTTGTACCTGGTGCTAATGTTTTGGGCATGGGCAATGGTACTGTCAAAGGTGGCTCCCCTAGAGCCCGTGTTGCGTCCTATAAGGTTTGTTGGCCAGACGAAACCAACGAGTGTGTGGACCCAAATACCTTAGCTGCCTTTGAAGCTGCAATTGAGGATGGCGTTGATGTTATCTCGATTTCTGTTGGTGGAGAGCCCAAAGAGTTCTTTAGTGATGCACTCTCTGTAGGGGCATTCCATGCAGTTGAGCGAGGTATTGTTGTTGTTTCCTCTGCTGGGAACGTGGGACCAACTCCTGGGACTGTATCAAATGTGTCGCCATGGATTCTAACTGTTGGAGCTAGTACTATTGATAGAGGTTTCACCAATTTTGTGGTCCTGGGGAATAAGAAGAAATTCAAGGTAACTCACATTCAATGCATTCAAAAGTCAAAAAACTCAATATGAATAATATTCTTTATCTTAAATATCTCTAAATTGCCTAAACCAGGGAACAAGCTTTTCTTCTAAGGTGCTGCCAGTTAACAAGTTCTACCCTTTAATCAATGCTGTGGATGCAAAAGCCAACAATGTCTCCGTTAGTGATGCGTGAGTATATATGTATATACATTTATTCGATTCCTTTTTTTCCTACATATTGGGAATGAATAATTAAACAATGCATAAATGTGTTATTGCAGAGAAGTTTGCGACGAGGGCTCACTTGATCCCGAAAAGTTAGCTGGGAAGATTGTGGTTTGCCTTCGAGGAGGTCTTCCGAGAGTATCAAAGGGCTATGTAGCTGCCAAGGCAGGGGCTGTTGGAATGCTCGTGGTCAACGATGAGGAAAGTGGGAATGCAATTTTAACTGATTCACATGTCCTTCCAGCTTCTCATGTAACCTACGATGACAGCATATCCATCTTCCAATACATCAATTCTACCAAGTATTTCCCTATATTTCCCCATCACTTCTCAAGATTATTCCTAAATATTTTGTCATTCTGTAAGTTTTAAGTCATAATAACAACTACTTTGATCAGGACACCAATGGCTTACATCAGTTCTGTGATGACAGAACTGGAAATCACACCATCCCCAGTAGTGGCTGATTTCTCATCAAGAGGCCCCAATACAATAGAGGAGTCAATCCTTAAGGTTTTCTTTTTCTCAATGTGTTACAAAATGTTTCAGTTAACAATTCATAAGTTCAAATCTGGGTGAATTTAATCTTAAAGAAGAAAACCATGATGACAAGTGACAACCACGATCAAAATAATCATATATTGTTGTGTTTTTATCAGCCTGATATAATAGCACCTGGTGTGAATATACTGGCGGCTTACCCCGACGGCATACCATTGACAGAAGCACCATTGGACGATCGTCAATCTCCTTTTAAAGTAGATTCTGGCACATCCATGGCCTGCCCCCATATTGCTGGCATTGTAGGCCTTCTCAAAACCCTAAACCCCAAATGGAGTCCAGCAGCTATTAAATCTGCAATCATGACAACAGGTTCATGTTCAATCAAACAATCAAATCAAATAAAAAACACAAAAAACAAGTTCCATCTCTCCTATATCCTTCTTCCCGTATACTAAAAAACTTTCAATCAATTTCATCTATGCAGCCAAAACAACAGACAACAATTTCAATCCAATTGTAGACTACGGAGGACTCGAAGCAAACCCATTAGCATATGGTGCCGGACATGTTAATCCAAACAGTGCAATGGACCCTGGGCTAGTTTACGACATTACAATCGACGATTACCTCAATTTCTTATGTGCTAGAGGCTACAATACAACACAAATAAAAAGAATATCAAAGAAGAACTTTGTTTGTGATAAGTCATTCAAAGTGACGGATTTGAATTACCCATCAATCTCAGTTACAAATCTGAAAATGGGTCCTGTGGCGATCAATCGAAAATTGAAGAATGTGGGAAGTCCAGGGACATACGTTGCTAGAGTGAAGACGCCATTGGAAGTTTCAATCATTGTTGAGCCAAGAATATTAGATTTTACTGCTATGGATGAAGAGAAAAGCTTCAAGGTGTTGTTGAATAGAAGTGGAAAGGGAAAGCAAGAAGGTTATGTGTTTGGGGAATTAGTATGGACTGATGTCAATCGCCATGTTAGGACCCCAATTGTTGTGAATTTAGGAGAATAA

mRNA sequence

ATGCCTTCCGAAAAACAAACGGCCCCTAACACTGTTCATGTAATTGGGATTATAGGTGTTTGGCCAGAATCTAAGAGCTTTAGTGATGAAGGCTATGGACCTATCCCCCCAAGGTGGATGGGAACTTGTCAAAGTGATGCCGACCCCAAGTTTCGTTGTAATAGAAAATTGATTGGAGCAAGATTCTTCAACATAGGCTATGGTGAACTTACTGATACCTTCAATTCTTCAAGAGACAATGTAGGGCATGGAACCCACACTTTATCCATAGCTGGTGGCAATTTTGTACCTGGTGCTAATGTTTTGGGCATGGGCAATGGTACTGTCAAAGGTGGCTCCCCTAGAGCCCGTGTTGCGTCCTATAAGGTTTGTTGGCCAGACGAAACCAACGAGTGTGTGGACCCAAATACCTTAGCTGCCTTTGAAGCTGCAATTGAGGATGGCGTTGATGTTATCTCGATTTCTGTTGGTGGAGAGCCCAAAGAGTTCTTTAGTGATGCACTCTCTGTAGGGGCATTCCATGCAGTTGAGCGAGGTATTGTTGTTGTTTCCTCTGCTGGGAACGTGGGACCAACTCCTGGGACTGTATCAAATGTGTCGCCATGGATTCTAACTGTTGGAGCTAGTACTATTGATAGAGGTTTCACCAATTTTGTGGTCCTGGGGAATAAGAAGAAATTCAAGGTAACTCACATTCAATGCATTCAAAAAGAAGTTTGCGACGAGGGCTCACTTGATCCCGAAAAGTTAGCTGGGAAGATTGTGGTTTGCCTTCGAGGAGGTCTTCCGAGAGTATCAAAGGGCTATGTAGCTGCCAAGGCAGGGGCTGTTGGAATGCTCGTGGTCAACGATGAGGAAAGTGGGAATGCAATTTTAACTGATTCACATGTCCTTCCAGCTTCTCATGTAACCTACGATGACAGCATATCCATCTTCCAATACATCAATTCTACCAAGACACCAATGGCTTACATCAGTTCTGTGATGACAGAACTGGAAATCACACCATCCCCAGTAGTGGCTGATTTCTCATCAAGAGGCCCCAATACAATAGAGGAGTCAATCCTTAAGCCTGATATAATAGCACCTGGTGTGAATATACTGGCGGCTTACCCCGACGGCATACCATTGACAGAAGCACCATTGGACGATCGTCAATCTCCTTTTAAAGTAGATTCTGGCACATCCATGGCCTGCCCCCATATTGCTGGCATTGTAGGCCTTCTCAAAACCCTAAACCCCAAATGGAGTCCAGCAGCTATTAAATCTGCAATCATGACAACAGCCAAAACAACAGACAACAATTTCAATCCAATTGTAGACTACGGAGGACTCGAAGCAAACCCATTAGCATATGGTGCCGGACATGTTAATCCAAACAGTGCAATGGACCCTGGGCTAGTTTACGACATTACAATCGACGATTACCTCAATTTCTTATGTGCTAGAGGCTACAATACAACACAAATAAAAAGAATATCAAAGAAGAACTTTGTTTGTGATAAGTCATTCAAAGTGACGGATTTGAATTACCCATCAATCTCAGTTACAAATCTGAAAATGGGTCCTGTGGCGATCAATCGAAAATTGAAGAATGTGGGAAGTCCAGGGACATACGTTGCTAGAGTGAAGACGCCATTGGAAGTTTCAATCATTGTTGAGCCAAGAATATTAGATTTTACTGCTATGGATGAAGAGAAAAGCTTCAAGGTGTTGTTGAATAGAAGTGGAAAGGGAAAGCAAGAAGGTTATGTGTTTGGGGAATTAGTATGGACTGATGTCAATCGCCATGTTAGGACCCCAATTGTTGTGAATTTAGGAGAATAA

Coding sequence (CDS)

ATGCCTTCCGAAAAACAAACGGCCCCTAACACTGTTCATGTAATTGGGATTATAGGTGTTTGGCCAGAATCTAAGAGCTTTAGTGATGAAGGCTATGGACCTATCCCCCCAAGGTGGATGGGAACTTGTCAAAGTGATGCCGACCCCAAGTTTCGTTGTAATAGAAAATTGATTGGAGCAAGATTCTTCAACATAGGCTATGGTGAACTTACTGATACCTTCAATTCTTCAAGAGACAATGTAGGGCATGGAACCCACACTTTATCCATAGCTGGTGGCAATTTTGTACCTGGTGCTAATGTTTTGGGCATGGGCAATGGTACTGTCAAAGGTGGCTCCCCTAGAGCCCGTGTTGCGTCCTATAAGGTTTGTTGGCCAGACGAAACCAACGAGTGTGTGGACCCAAATACCTTAGCTGCCTTTGAAGCTGCAATTGAGGATGGCGTTGATGTTATCTCGATTTCTGTTGGTGGAGAGCCCAAAGAGTTCTTTAGTGATGCACTCTCTGTAGGGGCATTCCATGCAGTTGAGCGAGGTATTGTTGTTGTTTCCTCTGCTGGGAACGTGGGACCAACTCCTGGGACTGTATCAAATGTGTCGCCATGGATTCTAACTGTTGGAGCTAGTACTATTGATAGAGGTTTCACCAATTTTGTGGTCCTGGGGAATAAGAAGAAATTCAAGGTAACTCACATTCAATGCATTCAAAAAGAAGTTTGCGACGAGGGCTCACTTGATCCCGAAAAGTTAGCTGGGAAGATTGTGGTTTGCCTTCGAGGAGGTCTTCCGAGAGTATCAAAGGGCTATGTAGCTGCCAAGGCAGGGGCTGTTGGAATGCTCGTGGTCAACGATGAGGAAAGTGGGAATGCAATTTTAACTGATTCACATGTCCTTCCAGCTTCTCATGTAACCTACGATGACAGCATATCCATCTTCCAATACATCAATTCTACCAAGACACCAATGGCTTACATCAGTTCTGTGATGACAGAACTGGAAATCACACCATCCCCAGTAGTGGCTGATTTCTCATCAAGAGGCCCCAATACAATAGAGGAGTCAATCCTTAAGCCTGATATAATAGCACCTGGTGTGAATATACTGGCGGCTTACCCCGACGGCATACCATTGACAGAAGCACCATTGGACGATCGTCAATCTCCTTTTAAAGTAGATTCTGGCACATCCATGGCCTGCCCCCATATTGCTGGCATTGTAGGCCTTCTCAAAACCCTAAACCCCAAATGGAGTCCAGCAGCTATTAAATCTGCAATCATGACAACAGCCAAAACAACAGACAACAATTTCAATCCAATTGTAGACTACGGAGGACTCGAAGCAAACCCATTAGCATATGGTGCCGGACATGTTAATCCAAACAGTGCAATGGACCCTGGGCTAGTTTACGACATTACAATCGACGATTACCTCAATTTCTTATGTGCTAGAGGCTACAATACAACACAAATAAAAAGAATATCAAAGAAGAACTTTGTTTGTGATAAGTCATTCAAAGTGACGGATTTGAATTACCCATCAATCTCAGTTACAAATCTGAAAATGGGTCCTGTGGCGATCAATCGAAAATTGAAGAATGTGGGAAGTCCAGGGACATACGTTGCTAGAGTGAAGACGCCATTGGAAGTTTCAATCATTGTTGAGCCAAGAATATTAGATTTTACTGCTATGGATGAAGAGAAAAGCTTCAAGGTGTTGTTGAATAGAAGTGGAAAGGGAAAGCAAGAAGGTTATGTGTTTGGGGAATTAGTATGGACTGATGTCAATCGCCATGTTAGGACCCCAATTGTTGTGAATTTAGGAGAATAA

Protein sequence

MPSEKQTAPNTVHVIGIIGVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKVTHIQCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDVNRHVRTPIVVNLGE*

Homology

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BLAST of CSPI03G03930 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 5.7e-184
Identity = 332/618 (53.72%), Postives = 420/618 (67.96%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTD-----T 78
           GVWPESKSFSDEGYG +P RW G C  D      CNRKLIGAR+FN GY   T      +
Sbjct: 165 GVWPESKSFSDEGYGAVPARWKGRCHKDVP----CNRKLIGARYFNKGYLAYTGLPSNAS 224

Query: 79  FNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPD-ETNEC 138
           + + RD+ GHG+HTLS A GNFVPGANV G+GNGT  GGSP+ARVA+YKVCWP  +  EC
Sbjct: 225 YETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAEC 284

Query: 139 VDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPT 198
            D + LAA EAAIEDGVDV+S SVGG+  ++ SD +++G+FHAV+ G+ VV SAGN GP 
Sbjct: 285 FDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPK 344

Query: 199 PGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKVTHIQCIQKE-------------- 258
            GTVSNV+PW++TVGAS++DR F  FV L N + FK T +     E              
Sbjct: 345 SGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPEEKMYSLISAADANV 404

Query: 259 ---------VCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGN 318
                    +C +GSLDP+K+ GKI+VCLRG   RV KG  AA AGA GM++ ND+ SGN
Sbjct: 405 ANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGN 464

Query: 319 AILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPN 378
            I++D+HVLPAS + Y D  ++F Y++STK P  YI +    L   P+P +A FSSRGPN
Sbjct: 465 EIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPN 524

Query: 379 TIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLL 438
           TI   ILKPDI APGVNI+AA+ +    T+   D+R++PF  +SGTSM+CPHI+G+VGLL
Sbjct: 525 TITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLL 584

Query: 439 KTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVY 498
           KTL+P WSPAAI+SAIMTT++T +N   P+VD    +ANP +YG+GHV PN A  PGLVY
Sbjct: 585 KTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVY 644

Query: 499 DITIDDYLNFLCARGYNTTQIKRISK-KNFVCDKSFKVTDLNYPSISVTNLKMGPVAINR 558
           D+T  DYL+FLCA GYN T ++  ++   + C +   + D NYPSI+V NL  G + + R
Sbjct: 645 DLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNL-TGSITVTR 704

Query: 559 KLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGE 607
           KLKNVG P TY AR + PL V + VEP+ L F    E K F++ L R       GYVFGE
Sbjct: 705 KLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTL-RPLPVTPSGYVFGE 764

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BLAST of CSPI03G03930 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 7.0e-182
Identity = 323/618 (52.27%), Postives = 414/618 (66.99%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGY----GELTDTF 78
           GVWPESKSF DEG GPIP RW G CQ+  D  F CNRKLIGAR+FN GY    G L  +F
Sbjct: 155 GVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSF 214

Query: 79  NSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPD-ETNECV 138
           +S RD  GHG+HTLS A G+FVPG ++ G GNGT KGGSPRARVA+YKVCWP  + NEC 
Sbjct: 215 DSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECY 274

Query: 139 DPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTP 198
           D + LAAF+AAI DG DVIS+S+GGEP  FF+D++++G+FHA ++ IVVV SAGN GP  
Sbjct: 275 DADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPAD 334

Query: 199 GTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKF------------------------KV 258
            TVSNV+PW +TVGAST+DR F + +VLGN K +                        K 
Sbjct: 335 STVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKA 394

Query: 259 THIQCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGN 318
            +   +  ++C  GSLDP K  GKI+VCLRG   RV KG   A  G +GM++ N   +GN
Sbjct: 395 KNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGN 454

Query: 319 AILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPN 378
            +L D HVLPA+ +T  DS ++ +YI+ TK P+A+I+   T+L + P+PV+A FSS+GP+
Sbjct: 455 DLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPS 514

Query: 379 TIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLL 438
            +   ILKPDI APGV+++AAY   +  T    D R+  F   SGTSM+CPHI+GI GLL
Sbjct: 515 IVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLL 574

Query: 439 KTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVY 498
           KT  P WSPAAI+SAIMTTA   D+   PI +   ++A P ++GAGHV PN A++PGLVY
Sbjct: 575 KTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVY 634

Query: 499 DITIDDYLNFLCARGYNTTQIKRISKKNFVCDK-SFKVTDLNYPSISVTNLKMGPVAINR 558
           D+ I DYLNFLC+ GYN +QI   S  NF C      + +LNYPSI+V NL    V ++R
Sbjct: 635 DLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSR 694

Query: 559 KLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGE 607
            +KNVG P  Y  +V  P  V + V+P  L+FT + E+K+FKV+L +S     +GYVFGE
Sbjct: 695 TVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGE 754

[Close]

BLAST of CSPI03G03930 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 523.5 bits (1347), Expect = 3.3e-147
Identity = 295/642 (45.95%), Postives = 396/642 (61.68%), Query Frame = 0


Query: 14  VIGII--GVWPESKSFSDEGYGPIPPRWM-GTCQSDADP---KFRCNRKLIGARFFNIGY 73
           +IG I  GVWPES+SFSD+GYG +P +W  G CQ +  P   K  CNRKLIGAR++N  +
Sbjct: 145 IIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAF 204

Query: 74  ----GELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKV 133
               G+L    +++RD VGHGTHTLS AGGNFVPGA V  +GNGT KGGSPRARVA+YKV
Sbjct: 205 EAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKV 264

Query: 134 CWP-DETNECVDPNTLAAFEAAIEDGVDVISISVGGE----PKEFFSDALSVGAFHAVER 193
           CW   +   C   + LAA + AI+DGVDVI++S G       +  F+D +S+GAFHA+ +
Sbjct: 265 CWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISK 324

Query: 194 GIVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNK-------------- 253
            I++V+SAGN GPTPGTV+NV+PW+ T+ AST+DR F++ + + N+              
Sbjct: 325 NILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINNQLIEGASLFVNLPPN 384

Query: 254 --------KKFKVTHIQCIQKEVCDEGSLDPEKLAGKIVVCLR-GGLPRVSKGYVAAKAG 313
                      K+ +      ++C  G+LD  K+ GKIV+C R G +  V++G  A  AG
Sbjct: 385 QAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAG 444

Query: 314 AVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINST---------KT-PMAYI 373
           A GM++ N  ++G  +  + HV    +     + S    + +T         KT     +
Sbjct: 445 ARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKM 504

Query: 374 SSVMTELEITPSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDR 433
           S   T     P+PV+A FSSRGPN I+ SILKPD+ APGVNILAAY +    +   +D+R
Sbjct: 505 SRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNR 564

Query: 434 QS-PFKVDSGTSMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVD-YG 493
           +   F V  GTSM+CPH +GI GLLKT +P WSPAAIKSAIMTTA T DN   PI D + 
Sbjct: 565 RGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFD 624

Query: 494 GLEANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRIS-KKNFVCDK 553
              A+  AYG+GHV P+ A++PGLVYD+++ DYLNFLCA GY+   I  ++  + F+C  
Sbjct: 625 KTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSG 684

Query: 554 SFKVTDLNYPSISVTNLKMGPVAINRKLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTA 605
           S  V DLNYPSI++ NL++ PV I R + NVG P TY    ++P   SI V P  L FT 
Sbjct: 685 SHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTK 744

[Close]

BLAST of CSPI03G03930 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 9.8e-144
Identity = 277/625 (44.32%), Postives = 384/625 (61.44%), Query Frame = 0


Query: 14  VIGII--GVWPESKSFSDEGYGPIPPRWMGTCQSDAD-PKFRCNRKLIGARFFNIGY--- 73
           V+G++  GVWPESKS+SDEG+GPIP  W G C++  +     CNRKLIGARFF  GY   
Sbjct: 134 VVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYEST 193

Query: 74  -GELTDT--FNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVC 133
            G + ++    S RD+ GHGTHT S A G+ V GA++LG  +GT +G +PRARVA YKVC
Sbjct: 194 MGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVC 253

Query: 134 WPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVS 193
           W      C   + LAA + AI D V+V+S+S+GG   +++ D +++GAF A+ERGI+V  
Sbjct: 254 W---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSC 313

Query: 194 SAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKVTHI------------ 253
           SAGN GP+  ++SNV+PWI TVGA T+DR F    +LGN K F    +            
Sbjct: 314 SAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLP 373

Query: 254 --------QCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVND 313
                         +C  G+L PEK+ GKIV+C RG   RV KG V   AG VGM++ N 
Sbjct: 374 FIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANT 433

Query: 314 EESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFS 373
             +G  ++ D+H+LPA+ V       I  Y+ +   P A IS + T + + PSPVVA FS
Sbjct: 434 AANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFS 493

Query: 374 SRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAG 433
           SRGPN+I  +ILKPD+IAPGVNILAA+      T    D R+  F + SGTSM+CPH++G
Sbjct: 494 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 553

Query: 434 IVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYG-GLEANPLAYGAGHVNPNSAM 493
           +  LLK+++P+WSPAAI+SA+MTTA  T  +  P++D   G  + P  +GAGHV+P +A 
Sbjct: 554 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTAT 613

Query: 494 DPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCD--KSFKVTDLNYPSISVTNLKM 553
           +PGL+YD+T +DYL FLCA  Y + QI+ +S++N+ CD  KS+ V DLNYPS +V    +
Sbjct: 614 NPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV 673

Query: 554 GPVAINRKLKNVGSPGTYVARVKTPLE-VSIIVEPRILDFTAMDEEKSFKVLLNRSGKGK 606
           G     R + +VG  GTY  +V +    V I VEP +L+F   +E+KS+ V         
Sbjct: 674 GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKP 733

[Close]

BLAST of CSPI03G03930 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 6.2e-138
Identity = 284/643 (44.17%), Postives = 384/643 (59.72%), Query Frame = 0


Query: 14  VIGII--GVWPESKSFSDEGYGPIPPRWMGTCQSDAD-PKFRCNRKLIGARFFNIGY--- 73
           VIG+I  GVWPE  SF D G GP+P +W G C +  D P+  CNRKL+GARFF  GY   
Sbjct: 132 VIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEAT 191

Query: 74  -GELTDT--FNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVC 133
            G++ +T  F S RD+ GHGTHT SI+ G +V  A+ LG  +G   G +P+AR+A+YKVC
Sbjct: 192 NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVC 251

Query: 134 WPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVS 193
           W    + C D + LAAF+ A+ DGVDVIS+SVGG    ++ DA+++GAF A++RGI V +
Sbjct: 252 W---NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSA 311

Query: 194 SAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKVTHI------------ 253
           SAGN GP   TV+NV+PW+ TVGA TIDR F   V LGN K      +            
Sbjct: 312 SAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMY 371

Query: 254 -----------QCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLV 313
                            +C EGSLDP  + GKIV+C RG   R +KG +  K G +GM++
Sbjct: 372 PLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMII 431

Query: 314 VNDEESGNAILTDSHVLPASHVTYDDSISIFQYIN------STKTPMAYISSVMTELEIT 373
            N    G  ++ D HVLPA+ V       I +YI+      S+K P A I    T L I 
Sbjct: 432 ANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIR 491

Query: 374 PSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGT 433
           P+PVVA FS+RGPN     ILKPD+IAPG+NILAA+PD I  +    D+R++ F + SGT
Sbjct: 492 PAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGT 551

Query: 434 SMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYG-GLEANPLAYGA 493
           SMACPH++G+  LLK  +P WSPAAI+SA++TTA T DN+  P++D   G  ++ + YG+
Sbjct: 552 SMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGS 611

Query: 494 GHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCD---KSFKVTDLNY 553
           GHV+P  AMDPGLVYDIT  DY+NFLC   Y  T I  I+++   CD   ++  V +LNY
Sbjct: 612 GHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNY 671

Query: 554 PSISVTNLKMGPVAIN----RKLKNVG-SPGTYVARVKTPLEVSIIVEPRILDFTAMDEE 607
           PS SV   + G   ++    R + NVG S   Y  +++ P   ++ VEP  L F  + ++
Sbjct: 672 PSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQK 731

[Close]

BLAST of CSPI03G03930 vs. ExPASy TrEMBL
Match: A0A1S3BL27 (subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491006 PE=3 SV=1)

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 552/614 (89.90%), Postives = 567/614 (92.35%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNSSR 78
           GVWPESKSFSDEGYGPIP RWMGTCQSDADPKFRCNRKLIGARFFNIGYGEL DTFNSSR
Sbjct: 68  GVWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELADTFNSSR 127

Query: 79  DNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPNTL 138
           DNVGHGTHTLSIAGGNFVPGANVLG+GNGTVKGGSPRARVASYKVCWPDE NEC+DPNTL
Sbjct: 128 DNVGHGTHTLSIAGGNFVPGANVLGLGNGTVKGGSPRARVASYKVCWPDEANECMDPNTL 187

Query: 139 AAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN 198
           AAFEAAI+DGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN
Sbjct: 188 AAFEAAIDDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN 247

Query: 199 VSPWILTVGASTIDRGFTNFVVLGNKKKFKVT------------------------HIQC 258
           VSPWILTVGAST DRGFTNFV+LGNKKKFK T                        ++  
Sbjct: 248 VSPWILTVGASTTDRGFTNFVILGNKKKFKGTSFSSKVLPVNKFYPLINAVDAKAKNVSV 307

Query: 259 IQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTD 318
              EVCDEGSLDP+KLAGKIVVCLRGGL RVSKGYVAAKAGAVGM+V NDE+SGNAI+TD
Sbjct: 308 SDAEVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITD 367

Query: 319 SHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIEES 378
           SHVLPASHVTYDDSISIFQYINSTK P AYISSVMTELEITPS VVADFSSRGPNTIEES
Sbjct: 368 SHVLPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGPNTIEES 427

Query: 379 ILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNP 438
           ILKPDI APGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPH+AGIVGLLKTLNP
Sbjct: 428 ILKPDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNP 487

Query: 439 KWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITID 498
           KWSPAAIKSAIMTTAKTTDNNFNPIVD+ GLEANPLAYGAGHVNPNSAMDPGLVYDITID
Sbjct: 488 KWSPAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITID 547

Query: 499 DYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 558
           DYLNFLCARGYNT QIKRISKKNF+CDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG
Sbjct: 548 DYLNFLCARGYNTKQIKRISKKNFICDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 607

Query: 559 SPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDV 609
           SPG YVARVKTPLEVSIIVEPRIL+FTAMDEEKSFKVLL RSGKGKQEGYVFGELVWTD 
Sbjct: 608 SPGRYVARVKTPLEVSIIVEPRILEFTAMDEEKSFKVLLKRSGKGKQEGYVFGELVWTDF 667

[Close]

BLAST of CSPI03G03930 vs. ExPASy TrEMBL
Match: A0A5D3D737 (Subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00080 PE=3 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 551/614 (89.74%), Postives = 567/614 (92.35%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNSSR 78
           GVWPESKSFSDEGYGPIP RWMGTCQSDADPKFRCNRKLIGARFFNIGYGEL DTFNSSR
Sbjct: 135 GVWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELADTFNSSR 194

Query: 79  DNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPNTL 138
           DNVGHGTHTLSIAGGNFVPGANVLG+GNGTVKGGSPRARVASYKVCWPDE NEC+DPNTL
Sbjct: 195 DNVGHGTHTLSIAGGNFVPGANVLGLGNGTVKGGSPRARVASYKVCWPDEANECMDPNTL 254

Query: 139 AAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN 198
           AAFEAAI+DGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGN+GPTPGTVSN
Sbjct: 255 AAFEAAIDDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNMGPTPGTVSN 314

Query: 199 VSPWILTVGASTIDRGFTNFVVLGNKKKFKVT------------------------HIQC 258
           VSPWILTVGAST DRGFTNFV+LGNKKKFK T                        ++  
Sbjct: 315 VSPWILTVGASTTDRGFTNFVILGNKKKFKGTSFSSKVLPVNKFYPLINAVDAKAKNVSV 374

Query: 259 IQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTD 318
              EVCDEGSLDP+KLAGKIVVCLRGGL RVSKGYVAAKAGAVGM+V NDE+SGNAI+TD
Sbjct: 375 SDAEVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITD 434

Query: 319 SHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIEES 378
           SHVLPASHVTYDDSISIFQYINSTK P AYISSVMTELEITPS VVADFSSRGPNTIEES
Sbjct: 435 SHVLPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGPNTIEES 494

Query: 379 ILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNP 438
           ILKPDI APGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPH+AGIVGLLKTLNP
Sbjct: 495 ILKPDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNP 554

Query: 439 KWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITID 498
           KWSPAAIKSAIMTTAKTTDNNFNPIVD+ GLEANPLAYGAGHVNPNSAMDPGLVYDITID
Sbjct: 555 KWSPAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITID 614

Query: 499 DYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 558
           DYLNFLCARGYNT QIKRISKKNF+CDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG
Sbjct: 615 DYLNFLCARGYNTKQIKRISKKNFICDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 674

Query: 559 SPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDV 609
           SPG YVARVKTPLEVSIIVEPRIL+FTAMDEEKSFKVLL RSGKGKQEGYVFGELVWTD 
Sbjct: 675 SPGRYVARVKTPLEVSIIVEPRILEFTAMDEEKSFKVLLKRSGKGKQEGYVFGELVWTDF 734

[Close]

BLAST of CSPI03G03930 vs. ExPASy TrEMBL
Match: A0A1S3BLZ2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491006 PE=3 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 552/643 (85.85%), Postives = 567/643 (88.18%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCN------------------------ 78
           GVWPESKSFSDEGYGPIP RWMGTCQSDADPKFRCN                        
Sbjct: 68  GVWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRSRDSCPISVFFSSLSIISSKFKN 127

Query: 79  -----RKLIGARFFNIGYGELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTV 138
                RKLIGARFFNIGYGEL DTFNSSRDNVGHGTHTLSIAGGNFVPGANVLG+GNGTV
Sbjct: 128 GYIKCRKLIGARFFNIGYGELADTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGLGNGTV 187

Query: 139 KGGSPRARVASYKVCWPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALS 198
           KGGSPRARVASYKVCWPDE NEC+DPNTLAAFEAAI+DGVDVISISVGGEPKEFFSDALS
Sbjct: 188 KGGSPRARVASYKVCWPDEANECMDPNTLAAFEAAIDDGVDVISISVGGEPKEFFSDALS 247

Query: 199 VGAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKV 258
           VGAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGAST DRGFTNFV+LGNKKKFK 
Sbjct: 248 VGAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGASTTDRGFTNFVILGNKKKFKG 307

Query: 259 T------------------------HIQCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRV 318
           T                        ++     EVCDEGSLDP+KLAGKIVVCLRGGL RV
Sbjct: 308 TSFSSKVLPVNKFYPLINAVDAKAKNVSVSDAEVCDEGSLDPKKLAGKIVVCLRGGLSRV 367

Query: 319 SKGYVAAKAGAVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYI 378
           SKGYVAAKAGAVGM+V NDE+SGNAI+TDSHVLPASHVTYDDSISIFQYINSTK P AYI
Sbjct: 368 SKGYVAAKAGAVGMIVENDEDSGNAIITDSHVLPASHVTYDDSISIFQYINSTKRPKAYI 427

Query: 379 SSVMTELEITPSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDR 438
           SSVMTELEITPS VVADFSSRGPNTIEESILKPDI APGVNILAAYPDGIPLTEAPLDDR
Sbjct: 428 SSVMTELEITPSAVVADFSSRGPNTIEESILKPDITAPGVNILAAYPDGIPLTEAPLDDR 487

Query: 439 QSPFKVDSGTSMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGL 498
           QSPFKVDSGTSMACPH+AGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVD+ GL
Sbjct: 488 QSPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDHRGL 547

Query: 499 EANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCDKSFK 558
           EANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNT QIKRISKKNF+CDKSFK
Sbjct: 548 EANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTKQIKRISKKNFICDKSFK 607

Query: 559 VTDLNYPSISVTNLKMGPVAINRKLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDE 609
           VTDLNYPSISVTNLKMGPVAINRKLKNVGSPG YVARVKTPLEVSIIVEPRIL+FTAMDE
Sbjct: 608 VTDLNYPSISVTNLKMGPVAINRKLKNVGSPGRYVARVKTPLEVSIIVEPRILEFTAMDE 667

[Close]

BLAST of CSPI03G03930 vs. ExPASy TrEMBL
Match: A0A0A0L617 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045160 PE=3 SV=1)

HSP 1 Score: 1068.1 bits (2761), Expect = 1.3e-308
Identity = 541/573 (94.42%), Postives = 543/573 (94.76%), Query Frame = 0


Query: 60  ARFFNIGYGELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVA 119
           ARFFNIGYGELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVA
Sbjct: 99  ARFFNIGYGELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVA 158

Query: 120 SYKVCWPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERG 179
           SYKVCWPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERG
Sbjct: 159 SYKVCWPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERG 218

Query: 180 IVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKVT--------- 239
           IVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFK T         
Sbjct: 219 IVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKGTSFSSKVLPV 278

Query: 240 ---------------HIQCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAG 299
                          ++     EVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAG
Sbjct: 279 NKFYPLINAVDAKANNVSVSDAEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAG 338

Query: 300 AVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEIT 359
           AVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEIT
Sbjct: 339 AVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEIT 398

Query: 360 PSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGT 419
           PSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGT
Sbjct: 399 PSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGT 458

Query: 420 SMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAG 479
           SMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAG
Sbjct: 459 SMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAG 518

Query: 480 HVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSIS 539
           HVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSIS
Sbjct: 519 HVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSIS 578

Query: 540 VTNLKMGPVAINRKLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNR 599
           VTNLKMGPVAINRKLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNR
Sbjct: 579 VTNLKMGPVAINRKLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNR 638

Query: 600 SGKGKQEGYVFGELVWTDVNRHVRTPIVVNLGE 609
           SGKGKQEGYVFGELVWTDVNRHVRTPIVVNLGE
Sbjct: 639 SGKGKQEGYVFGELVWTDVNRHVRTPIVVNLGE 671

[Close]

BLAST of CSPI03G03930 vs. ExPASy TrEMBL
Match: A0A6J1KUM0 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498350 PE=3 SV=1)

HSP 1 Score: 921.4 bits (2380), Expect = 2.0e-264
Identity = 456/614 (74.27%), Postives = 504/614 (82.08%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNSSR 78
           GVWPE+KSFSDEGYGPIP RW GTCQSD+DP F CN+KLIGARFFN GYG L  TFNS R
Sbjct: 153 GVWPEAKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGYGNLNATFNSPR 212

Query: 79  DNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPNTL 138
           D  GHGTHTLSIAGGNFV GANV  M NGT KGGSPRAR+ASYKVCWP E  +C+DPN L
Sbjct: 213 DQEGHGTHTLSIAGGNFVSGANVFDMANGTAKGGSPRARIASYKVCWPVEGRQCLDPNAL 272

Query: 139 AAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN 198
           AA++AAI DGVDVIS+S+GGEPKEF  DALSVGAFHAV+ GIVVV SAGN GPTP TVSN
Sbjct: 273 AAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSN 332

Query: 199 VSPWILTVGASTIDRGFTNFVVLGNKKKFKVT------------------------HIQC 258
           VSPW+LTVGASTIDR FTNFVVLGNKKK K T                        +   
Sbjct: 333 VSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASS 392

Query: 259 IQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTD 318
              EVC+E SLDP KLAGKIVVCLRG + RVSKGYV A+AGA GM++VND+++G+AI TD
Sbjct: 393 SDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATD 452

Query: 319 SHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIEES 378
            H+LPASHVT++D ISIF YI STKTPMA ISSV TEL++ PSPV+ADFSSRGP+TIE S
Sbjct: 453 LHLLPASHVTFNDGISIFHYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGS 512

Query: 379 ILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNP 438
           ILKPDI APGVNI+AAYPD IPL E  +DDR++PFKVDSGTSMACPH+AGIVGLLK+  P
Sbjct: 513 ILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYP 572

Query: 439 KWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITID 498
           KWSPAAIKSAIMTTAKT+ NNFNPI+D+ GLEA PLAYG GHV+PNS MDPGLVYDI ID
Sbjct: 573 KWSPAAIKSAIMTTAKTSANNFNPILDFTGLEATPLAYGNGHVDPNSVMDPGLVYDIAID 632

Query: 499 DYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 558
           DYLNFLCARG N TQI ++S K FVCD SFKVTDLNYPSISVTNLK GPV INRKLKNVG
Sbjct: 633 DYLNFLCARGDNATQINKLSHKLFVCDPSFKVTDLNYPSISVTNLKTGPVTINRKLKNVG 692

Query: 559 SPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDV 609
           SPGTYVA+VK PLEVSI VEP  L FTAMDEEKSFK++L RSGKG QEGY FGEL W+D 
Sbjct: 693 SPGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYAFGELAWSDG 752

[Close]

BLAST of CSPI03G03930 vs. NCBI nr
Match: KAE8650099.1 (hypothetical protein Csa_011439 [Cucumis sativus])

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 581/616 (94.32%), Postives = 584/616 (94.81%), Query Frame = 0


Query: 17  IIGVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNS 76
           +  VWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNS
Sbjct: 104 LTSVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNS 163

Query: 77  SRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPN 136
           SRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPN
Sbjct: 164 SRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPN 223

Query: 137 TLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTV 196
           TLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTV
Sbjct: 224 TLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTV 283

Query: 197 SNVSPWILTVGASTIDRGFTNFVVLGNKKKFKVT------------------------HI 256
           SNVSPWILTVGASTIDRGFTNFVVLGNKKKFK T                        ++
Sbjct: 284 SNVSPWILTVGASTIDRGFTNFVVLGNKKKFKGTSFSSKVLPVNKFYPLINAVDAKANNV 343

Query: 257 QCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAIL 316
                EVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAIL
Sbjct: 344 SVSDAEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAIL 403

Query: 317 TDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIE 376
           TDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIE
Sbjct: 404 TDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIE 463

Query: 377 ESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTL 436
           ESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTL
Sbjct: 464 ESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTL 523

Query: 437 NPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDIT 496
           NPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDIT
Sbjct: 524 NPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDIT 583

Query: 497 IDDYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKN 556
           IDDYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKN
Sbjct: 584 IDDYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKN 643

Query: 557 VGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWT 609
           VGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWT
Sbjct: 644 VGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWT 703

[Close]

BLAST of CSPI03G03930 vs. NCBI nr
Match: XP_008449005.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X2 [Cucumis melo])

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 552/614 (89.90%), Postives = 567/614 (92.35%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNSSR 78
           GVWPESKSFSDEGYGPIP RWMGTCQSDADPKFRCNRKLIGARFFNIGYGEL DTFNSSR
Sbjct: 68  GVWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELADTFNSSR 127

Query: 79  DNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPNTL 138
           DNVGHGTHTLSIAGGNFVPGANVLG+GNGTVKGGSPRARVASYKVCWPDE NEC+DPNTL
Sbjct: 128 DNVGHGTHTLSIAGGNFVPGANVLGLGNGTVKGGSPRARVASYKVCWPDEANECMDPNTL 187

Query: 139 AAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN 198
           AAFEAAI+DGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN
Sbjct: 188 AAFEAAIDDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN 247

Query: 199 VSPWILTVGASTIDRGFTNFVVLGNKKKFKVT------------------------HIQC 258
           VSPWILTVGAST DRGFTNFV+LGNKKKFK T                        ++  
Sbjct: 248 VSPWILTVGASTTDRGFTNFVILGNKKKFKGTSFSSKVLPVNKFYPLINAVDAKAKNVSV 307

Query: 259 IQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTD 318
              EVCDEGSLDP+KLAGKIVVCLRGGL RVSKGYVAAKAGAVGM+V NDE+SGNAI+TD
Sbjct: 308 SDAEVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITD 367

Query: 319 SHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIEES 378
           SHVLPASHVTYDDSISIFQYINSTK P AYISSVMTELEITPS VVADFSSRGPNTIEES
Sbjct: 368 SHVLPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGPNTIEES 427

Query: 379 ILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNP 438
           ILKPDI APGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPH+AGIVGLLKTLNP
Sbjct: 428 ILKPDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNP 487

Query: 439 KWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITID 498
           KWSPAAIKSAIMTTAKTTDNNFNPIVD+ GLEANPLAYGAGHVNPNSAMDPGLVYDITID
Sbjct: 488 KWSPAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITID 547

Query: 499 DYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 558
           DYLNFLCARGYNT QIKRISKKNF+CDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG
Sbjct: 548 DYLNFLCARGYNTKQIKRISKKNFICDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 607

Query: 559 SPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDV 609
           SPG YVARVKTPLEVSIIVEPRIL+FTAMDEEKSFKVLL RSGKGKQEGYVFGELVWTD 
Sbjct: 608 SPGRYVARVKTPLEVSIIVEPRILEFTAMDEEKSFKVLLKRSGKGKQEGYVFGELVWTDF 667

[Close]

BLAST of CSPI03G03930 vs. NCBI nr
Match: TYK19379.1 (subtilisin-like protease SBT5.3 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 551/614 (89.74%), Postives = 567/614 (92.35%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNSSR 78
           GVWPESKSFSDEGYGPIP RWMGTCQSDADPKFRCNRKLIGARFFNIGYGEL DTFNSSR
Sbjct: 135 GVWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELADTFNSSR 194

Query: 79  DNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPNTL 138
           DNVGHGTHTLSIAGGNFVPGANVLG+GNGTVKGGSPRARVASYKVCWPDE NEC+DPNTL
Sbjct: 195 DNVGHGTHTLSIAGGNFVPGANVLGLGNGTVKGGSPRARVASYKVCWPDEANECMDPNTL 254

Query: 139 AAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN 198
           AAFEAAI+DGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGN+GPTPGTVSN
Sbjct: 255 AAFEAAIDDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNMGPTPGTVSN 314

Query: 199 VSPWILTVGASTIDRGFTNFVVLGNKKKFKVT------------------------HIQC 258
           VSPWILTVGAST DRGFTNFV+LGNKKKFK T                        ++  
Sbjct: 315 VSPWILTVGASTTDRGFTNFVILGNKKKFKGTSFSSKVLPVNKFYPLINAVDAKAKNVSV 374

Query: 259 IQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTD 318
              EVCDEGSLDP+KLAGKIVVCLRGGL RVSKGYVAAKAGAVGM+V NDE+SGNAI+TD
Sbjct: 375 SDAEVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSGNAIITD 434

Query: 319 SHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIEES 378
           SHVLPASHVTYDDSISIFQYINSTK P AYISSVMTELEITPS VVADFSSRGPNTIEES
Sbjct: 435 SHVLPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGPNTIEES 494

Query: 379 ILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNP 438
           ILKPDI APGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPH+AGIVGLLKTLNP
Sbjct: 495 ILKPDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGLLKTLNP 554

Query: 439 KWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITID 498
           KWSPAAIKSAIMTTAKTTDNNFNPIVD+ GLEANPLAYGAGHVNPNSAMDPGLVYDITID
Sbjct: 555 KWSPAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLVYDITID 614

Query: 499 DYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 558
           DYLNFLCARGYNT QIKRISKKNF+CDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG
Sbjct: 615 DYLNFLCARGYNTKQIKRISKKNFICDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 674

Query: 559 SPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDV 609
           SPG YVARVKTPLEVSIIVEPRIL+FTAMDEEKSFKVLL RSGKGKQEGYVFGELVWTD 
Sbjct: 675 SPGRYVARVKTPLEVSIIVEPRILEFTAMDEEKSFKVLLKRSGKGKQEGYVFGELVWTDF 734

[Close]

BLAST of CSPI03G03930 vs. NCBI nr
Match: XP_008449002.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 552/643 (85.85%), Postives = 567/643 (88.18%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCN------------------------ 78
           GVWPESKSFSDEGYGPIP RWMGTCQSDADPKFRCN                        
Sbjct: 68  GVWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRSRDSCPISVFFSSLSIISSKFKN 127

Query: 79  -----RKLIGARFFNIGYGELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTV 138
                RKLIGARFFNIGYGEL DTFNSSRDNVGHGTHTLSIAGGNFVPGANVLG+GNGTV
Sbjct: 128 GYIKCRKLIGARFFNIGYGELADTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGLGNGTV 187

Query: 139 KGGSPRARVASYKVCWPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALS 198
           KGGSPRARVASYKVCWPDE NEC+DPNTLAAFEAAI+DGVDVISISVGGEPKEFFSDALS
Sbjct: 188 KGGSPRARVASYKVCWPDEANECMDPNTLAAFEAAIDDGVDVISISVGGEPKEFFSDALS 247

Query: 199 VGAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKV 258
           VGAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGAST DRGFTNFV+LGNKKKFK 
Sbjct: 248 VGAFHAVERGIVVVSSAGNVGPTPGTVSNVSPWILTVGASTTDRGFTNFVILGNKKKFKG 307

Query: 259 T------------------------HIQCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRV 318
           T                        ++     EVCDEGSLDP+KLAGKIVVCLRGGL RV
Sbjct: 308 TSFSSKVLPVNKFYPLINAVDAKAKNVSVSDAEVCDEGSLDPKKLAGKIVVCLRGGLSRV 367

Query: 319 SKGYVAAKAGAVGMLVVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYI 378
           SKGYVAAKAGAVGM+V NDE+SGNAI+TDSHVLPASHVTYDDSISIFQYINSTK P AYI
Sbjct: 368 SKGYVAAKAGAVGMIVENDEDSGNAIITDSHVLPASHVTYDDSISIFQYINSTKRPKAYI 427

Query: 379 SSVMTELEITPSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDR 438
           SSVMTELEITPS VVADFSSRGPNTIEESILKPDI APGVNILAAYPDGIPLTEAPLDDR
Sbjct: 428 SSVMTELEITPSAVVADFSSRGPNTIEESILKPDITAPGVNILAAYPDGIPLTEAPLDDR 487

Query: 439 QSPFKVDSGTSMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGL 498
           QSPFKVDSGTSMACPH+AGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVD+ GL
Sbjct: 488 QSPFKVDSGTSMACPHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDHRGL 547

Query: 499 EANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCDKSFK 558
           EANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNT QIKRISKKNF+CDKSFK
Sbjct: 548 EANPLAYGAGHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTKQIKRISKKNFICDKSFK 607

Query: 559 VTDLNYPSISVTNLKMGPVAINRKLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDE 609
           VTDLNYPSISVTNLKMGPVAINRKLKNVGSPG YVARVKTPLEVSIIVEPRIL+FTAMDE
Sbjct: 608 VTDLNYPSISVTNLKMGPVAINRKLKNVGSPGRYVARVKTPLEVSIIVEPRILEFTAMDE 667

[Close]

BLAST of CSPI03G03930 vs. NCBI nr
Match: XP_038903594.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1017.3 bits (2629), Expect = 5.5e-293
Identity = 507/614 (82.57%), Postives = 542/614 (88.27%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTDTFNSSR 78
           GVWPESKSFSDEGYGPIP RW GTCQS  DPKF CNRKLIGARFFN+GYGEL+ TFNSS+
Sbjct: 153 GVWPESKSFSDEGYGPIPSRWKGTCQS--DPKFHCNRKLIGARFFNLGYGELSVTFNSSK 212

Query: 79  DNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPDETNECVDPNTL 138
           DNVGHGTHTLSIAGGNFV GANVLGMGNGT+KGGSPRARVASYKVCWP E +EC+DPNTL
Sbjct: 213 DNVGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKVCWPAEASECLDPNTL 272

Query: 139 AAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTPGTVSN 198
           AAFEAAI+DGVDVISISVG EPKEFFSDALSVGAFHAVERGIVVV SAGNVGPTPGTVSN
Sbjct: 273 AAFEAAIDDGVDVISISVGAEPKEFFSDALSVGAFHAVERGIVVVCSAGNVGPTPGTVSN 332

Query: 199 VSPWILTVGASTIDRGFTNFVVLGNKKKFKVT------------------------HIQC 258
           VSPWILTVGASTIDR FTNFVVLGNKKKFK T                        ++  
Sbjct: 333 VSPWILTVGASTIDRDFTNFVVLGNKKKFKGTSFSSKALPFNKLYPLINAVDAKANNVSN 392

Query: 259 IQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGNAILTD 318
              EVC+EGSLDPEKL GKIVVCLRGGLPRVSKGYVAAKAGA GM++VNDEESGNAILTD
Sbjct: 393 SDAEVCEEGSLDPEKLTGKIVVCLRGGLPRVSKGYVAAKAGAAGMILVNDEESGNAILTD 452

Query: 319 SHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPNTIEES 378
            H+LPASH+TY+DSISIFQYINSTKTPMAYISSVMTELEI PSPV+ADFSSRGPNTIEES
Sbjct: 453 LHILPASHITYNDSISIFQYINSTKTPMAYISSVMTELEIKPSPVMADFSSRGPNTIEES 512

Query: 379 ILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLLKTLNP 438
           ILKPDI APGVNI+AAYP+G+PLT+ PLDDRQ+PF VDSGTSMACPH+AGIVGLLKTLNP
Sbjct: 513 ILKPDITAPGVNIMAAYPNGVPLTKLPLDDRQAPFNVDSGTSMACPHVAGIVGLLKTLNP 572

Query: 439 KWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVYDITID 498
           KWSPAAIKSAIMTTAKT DN+ +PI+D  GL+A PLAYGAGHVNPNSAMDPGLVYDITID
Sbjct: 573 KWSPAAIKSAIMTTAKTRDNSLHPILDSTGLKATPLAYGAGHVNPNSAMDPGLVYDITID 632

Query: 499 DYLNFLCARGYNTTQIKRISKKNFVCDKSFKVTDLNYPSISVTNLKMGPVAINRKLKNVG 558
           DYLNFLCARGYN TQIKRISKK FVCD+SFKVTDLNYPSISVT LK G V INRK+KNVG
Sbjct: 633 DYLNFLCARGYNATQIKRISKKIFVCDRSFKVTDLNYPSISVTGLKTGHVTINRKVKNVG 692

Query: 559 SPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGELVWTDV 609
           SPGTYVARVK PLEVSI+VEP  L FTA+DEEKSFKVLL+ +GKG Q GYVFG+L W+D 
Sbjct: 693 SPGTYVARVKAPLEVSIMVEPSTLHFTAIDEEKSFKVLLHSTGKGNQNGYVFGKLAWSDG 752

[Close]

BLAST of CSPI03G03930 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 645.6 bits (1664), Expect = 4.0e-185
Identity = 332/618 (53.72%), Postives = 420/618 (67.96%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELTD-----T 78
           GVWPESKSFSDEGYG +P RW G C  D      CNRKLIGAR+FN GY   T      +
Sbjct: 165 GVWPESKSFSDEGYGAVPARWKGRCHKDVP----CNRKLIGARYFNKGYLAYTGLPSNAS 224

Query: 79  FNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPD-ETNEC 138
           + + RD+ GHG+HTLS A GNFVPGANV G+GNGT  GGSP+ARVA+YKVCWP  +  EC
Sbjct: 225 YETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAEC 284

Query: 139 VDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPT 198
            D + LAA EAAIEDGVDV+S SVGG+  ++ SD +++G+FHAV+ G+ VV SAGN GP 
Sbjct: 285 FDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPK 344

Query: 199 PGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKVTHIQCIQKE-------------- 258
            GTVSNV+PW++TVGAS++DR F  FV L N + FK T +     E              
Sbjct: 345 SGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPEEKMYSLISAADANV 404

Query: 259 ---------VCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGN 318
                    +C +GSLDP+K+ GKI+VCLRG   RV KG  AA AGA GM++ ND+ SGN
Sbjct: 405 ANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGN 464

Query: 319 AILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPN 378
            I++D+HVLPAS + Y D  ++F Y++STK P  YI +    L   P+P +A FSSRGPN
Sbjct: 465 EIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPN 524

Query: 379 TIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLL 438
           TI   ILKPDI APGVNI+AA+ +    T+   D+R++PF  +SGTSM+CPHI+G+VGLL
Sbjct: 525 TITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLL 584

Query: 439 KTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVY 498
           KTL+P WSPAAI+SAIMTT++T +N   P+VD    +ANP +YG+GHV PN A  PGLVY
Sbjct: 585 KTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVY 644

Query: 499 DITIDDYLNFLCARGYNTTQIKRISK-KNFVCDKSFKVTDLNYPSISVTNLKMGPVAINR 558
           D+T  DYL+FLCA GYN T ++  ++   + C +   + D NYPSI+V NL  G + + R
Sbjct: 645 DLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNL-TGSITVTR 704

Query: 559 KLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGE 607
           KLKNVG P TY AR + PL V + VEP+ L F    E K F++ L R       GYVFGE
Sbjct: 705 KLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTL-RPLPVTPSGYVFGE 764

[Close]

BLAST of CSPI03G03930 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 638.6 bits (1646), Expect = 4.9e-183
Identity = 323/618 (52.27%), Postives = 414/618 (66.99%), Query Frame = 0


Query: 19  GVWPESKSFSDEGYGPIPPRWMGTCQSDADPKFRCNRKLIGARFFNIGY----GELTDTF 78
           GVWPESKSF DEG GPIP RW G CQ+  D  F CNRKLIGAR+FN GY    G L  +F
Sbjct: 155 GVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSF 214

Query: 79  NSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVCWPD-ETNECV 138
           +S RD  GHG+HTLS A G+FVPG ++ G GNGT KGGSPRARVA+YKVCWP  + NEC 
Sbjct: 215 DSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECY 274

Query: 139 DPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNVGPTP 198
           D + LAAF+AAI DG DVIS+S+GGEP  FF+D++++G+FHA ++ IVVV SAGN GP  
Sbjct: 275 DADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPAD 334

Query: 199 GTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKF------------------------KV 258
            TVSNV+PW +TVGAST+DR F + +VLGN K +                        K 
Sbjct: 335 STVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKA 394

Query: 259 THIQCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVNDEESGN 318
            +   +  ++C  GSLDP K  GKI+VCLRG   RV KG   A  G +GM++ N   +GN
Sbjct: 395 KNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGN 454

Query: 319 AILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFSSRGPN 378
            +L D HVLPA+ +T  DS ++ +YI+ TK P+A+I+   T+L + P+PV+A FSS+GP+
Sbjct: 455 DLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPS 514

Query: 379 TIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAGIVGLL 438
            +   ILKPDI APGV+++AAY   +  T    D R+  F   SGTSM+CPHI+GI GLL
Sbjct: 515 IVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLL 574

Query: 439 KTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYGGLEANPLAYGAGHVNPNSAMDPGLVY 498
           KT  P WSPAAI+SAIMTTA   D+   PI +   ++A P ++GAGHV PN A++PGLVY
Sbjct: 575 KTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVY 634

Query: 499 DITIDDYLNFLCARGYNTTQIKRISKKNFVCDK-SFKVTDLNYPSISVTNLKMGPVAINR 558
           D+ I DYLNFLC+ GYN +QI   S  NF C      + +LNYPSI+V NL    V ++R
Sbjct: 635 DLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSR 694

Query: 559 KLKNVGSPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVLLNRSGKGKQEGYVFGE 607
            +KNVG P  Y  +V  P  V + V+P  L+FT + E+K+FKV+L +S     +GYVFGE
Sbjct: 695 TVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGE 754

[Close]

BLAST of CSPI03G03930 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 511.9 bits (1317), Expect = 7.0e-145
Identity = 277/625 (44.32%), Postives = 384/625 (61.44%), Query Frame = 0


Query: 14  VIGII--GVWPESKSFSDEGYGPIPPRWMGTCQSDAD-PKFRCNRKLIGARFFNIGY--- 73
           V+G++  GVWPESKS+SDEG+GPIP  W G C++  +     CNRKLIGARFF  GY   
Sbjct: 134 VVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYEST 193

Query: 74  -GELTDT--FNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVC 133
            G + ++    S RD+ GHGTHT S A G+ V GA++LG  +GT +G +PRARVA YKVC
Sbjct: 194 MGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVC 253

Query: 134 WPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVS 193
           W      C   + LAA + AI D V+V+S+S+GG   +++ D +++GAF A+ERGI+V  
Sbjct: 254 W---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSC 313

Query: 194 SAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKVTHI------------ 253
           SAGN GP+  ++SNV+PWI TVGA T+DR F    +LGN K F    +            
Sbjct: 314 SAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLP 373

Query: 254 --------QCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLVVND 313
                         +C  G+L PEK+ GKIV+C RG   RV KG V   AG VGM++ N 
Sbjct: 374 FIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANT 433

Query: 314 EESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVVADFS 373
             +G  ++ D+H+LPA+ V       I  Y+ +   P A IS + T + + PSPVVA FS
Sbjct: 434 AANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFS 493

Query: 374 SRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHIAG 433
           SRGPN+I  +ILKPD+IAPGVNILAA+      T    D R+  F + SGTSM+CPH++G
Sbjct: 494 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 553

Query: 434 IVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYG-GLEANPLAYGAGHVNPNSAM 493
           +  LLK+++P+WSPAAI+SA+MTTA  T  +  P++D   G  + P  +GAGHV+P +A 
Sbjct: 554 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTAT 613

Query: 494 DPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCD--KSFKVTDLNYPSISVTNLKM 553
           +PGL+YD+T +DYL FLCA  Y + QI+ +S++N+ CD  KS+ V DLNYPS +V    +
Sbjct: 614 NPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV 673

Query: 554 GPVAINRKLKNVGSPGTYVARVKTPLE-VSIIVEPRILDFTAMDEEKSFKVLLNRSGKGK 606
           G     R + +VG  GTY  +V +    V I VEP +L+F   +E+KS+ V         
Sbjct: 674 GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKP 733

[Close]

BLAST of CSPI03G03930 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 492.7 bits (1267), Expect = 4.4e-139
Identity = 284/643 (44.17%), Postives = 384/643 (59.72%), Query Frame = 0


Query: 14  VIGII--GVWPESKSFSDEGYGPIPPRWMGTCQSDAD-PKFRCNRKLIGARFFNIGY--- 73
           VIG+I  GVWPE  SF D G GP+P +W G C +  D P+  CNRKL+GARFF  GY   
Sbjct: 132 VIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEAT 191

Query: 74  -GELTDT--FNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYKVC 133
            G++ +T  F S RD+ GHGTHT SI+ G +V  A+ LG  +G   G +P+AR+A+YKVC
Sbjct: 192 NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVC 251

Query: 134 WPDETNECVDPNTLAAFEAAIEDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVS 193
           W    + C D + LAAF+ A+ DGVDVIS+SVGG    ++ DA+++GAF A++RGI V +
Sbjct: 252 W---NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSA 311

Query: 194 SAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLGNKKKFKVTHI------------ 253
           SAGN GP   TV+NV+PW+ TVGA TIDR F   V LGN K      +            
Sbjct: 312 SAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMY 371

Query: 254 -----------QCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGMLV 313
                            +C EGSLDP  + GKIV+C RG   R +KG +  K G +GM++
Sbjct: 372 PLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMII 431

Query: 314 VNDEESGNAILTDSHVLPASHVTYDDSISIFQYIN------STKTPMAYISSVMTELEIT 373
            N    G  ++ D HVLPA+ V       I +YI+      S+K P A I    T L I 
Sbjct: 432 ANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIR 491

Query: 374 PSPVVADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGT 433
           P+PVVA FS+RGPN     ILKPD+IAPG+NILAA+PD I  +    D+R++ F + SGT
Sbjct: 492 PAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGT 551

Query: 434 SMACPHIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYG-GLEANPLAYGA 493
           SMACPH++G+  LLK  +P WSPAAI+SA++TTA T DN+  P++D   G  ++ + YG+
Sbjct: 552 SMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGS 611

Query: 494 GHVNPNSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCD---KSFKVTDLNY 553
           GHV+P  AMDPGLVYDIT  DY+NFLC   Y  T I  I+++   CD   ++  V +LNY
Sbjct: 612 GHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNY 671

Query: 554 PSISVTNLKMGPVAIN----RKLKNVG-SPGTYVARVKTPLEVSIIVEPRILDFTAMDEE 607
           PS SV   + G   ++    R + NVG S   Y  +++ P   ++ VEP  L F  + ++
Sbjct: 672 PSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQK 731

[Close]

BLAST of CSPI03G03930 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 478.8 bits (1231), Expect = 6.5e-135
Identity = 277/636 (43.55%), Postives = 384/636 (60.38%), Query Frame = 0


Query: 14  VIGII--GVWPESKSFSDEGYGPIPPRWMGTCQSDA--DPKFRCNRKLIGARFFN----- 73
           +IG+   G+WPE +SFSD   GPIP RW G C+S A   P+  CNRK+IGARFF      
Sbjct: 126 IIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPR-NCNRKIIGARFFAKGQQA 185

Query: 74  --IGYGELTDTFNSSRDNVGHGTHTLSIAGGNFVPGANVLGMGNGTVKGGSPRARVASYK 133
             IG    T  F S RD  GHGTHT S A G     A++ G  +G  KG +P+AR+A+YK
Sbjct: 186 AVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYK 245

Query: 134 VCWPDETNECVDPNTLAAFEAAIEDGVDVISISVGGE---PKEFFSDALSVGAFHAVERG 193
           VCW D  + C+D + LAAF+AA+ DGVDVISIS+GG       ++ D +++G++ A  +G
Sbjct: 246 VCWKD--SGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKG 305

Query: 194 IVVVSSAGNVGPTPGTVSNVSPWILTVGASTIDRGFTNFVVLG----------------N 253
           I V SSAGN GP   +V+N++PW+ TVGASTIDR F    +LG                N
Sbjct: 306 IFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLN 365

Query: 254 KKKFKVTH---IQCIQKEVCDEGSLDPEKLAGKIVVCLRGGLPRVSKGYVAAKAGAVGML 313
            + F V +          +C E +LDP+++ GKIV+C RG  PRV+KG V  KAG VGM+
Sbjct: 366 GRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMI 425

Query: 314 VVNDEESGNAILTDSHVLPASHVTYDDSISIFQYINSTKTPMAYISSVMTELEITPSPVV 373
           + N   +G  ++ D+H++PA  V  ++   I  Y +S   P+A I    T + I P+PV+
Sbjct: 426 LANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVI 485

Query: 374 ADFSSRGPNTIEESILKPDIIAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACP 433
           A FS RGPN +   ILKPD+IAPGVNILAA+ D +  T  P D R++ F + SGTSMACP
Sbjct: 486 ASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACP 545

Query: 434 HIAGIVGLLKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDYG-GLEANPLAYGAGHVNP 493
           H++G   LLK+ +P WSPA I+SA+MTT    DN+   ++D   G  A P  YG+GH+N 
Sbjct: 546 HVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNL 605

Query: 494 NSAMDPGLVYDITIDDYLNFLCARGYNTTQIKRISKKNFVCDKSFKVT--DLNYPSISV- 553
             AM+PGLVYDIT DDY+ FLC+ GY    I+ I++    C  + K +  +LNYPSI+  
Sbjct: 606 GRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAV 665

Query: 554 --TNLK-MGPVAINRKLKNVG-SPGTYVARVKTPLEVSIIVEPRILDFTAMDEEKSFKVL 605
             TN + +    + R   NVG +   Y AR+++P  V++ V+P  L FT+  + +S+ V 
Sbjct: 666 FPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVT 725

The following BLAST results are available for this feature:

BLAST of CSPI03G03930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JXC5	5.7e-184	53.72	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
Q9ZSP5	7.0e-182	52.27	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
I1N462	3.3e-147	45.95	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
O65351	9.8e-144	44.32	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
Q9LUM3	6.2e-138	44.17	Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S...	[more]

BLAST of CSPI03G03930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BL27	0.0e+00	89.90	subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034910...	[more]
A0A5D3D737	0.0e+00	89.74	Subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BLZ2	0.0e+00	85.85	subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034910...	[more]
A0A0A0L617	1.3e-308	94.42	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045160 PE=3 SV=1	[more]
A0A6J1KUM0	2.0e-264	74.27	subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498350 PE=3...	[more]

BLAST of CSPI03G03930 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8650099.1	0.0e+00	94.32	hypothetical protein Csa_011439 [Cucumis sativus]	[more]
XP_008449005.1	0.0e+00	89.90	PREDICTED: subtilisin-like protease SBT5.3 isoform X2 [Cucumis melo]	[more]
TYK19379.1	0.0e+00	89.74	subtilisin-like protease SBT5.3 isoform X2 [Cucumis melo var. makuwa]	[more]
XP_008449002.1	0.0e+00	85.85	PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo]	[more]
XP_038903594.1	5.5e-293	82.57	subtilisin-like protease SBT5.4 [Benincasa hispida]	[more]

BLAST of CSPI03G03930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59810.1	4.0e-185	53.72	Subtilase family protein	[more]
AT2G04160.1	4.9e-183	52.27	Subtilisin-like serine endopeptidase family protein	[more]
AT5G67360.1	7.0e-145	44.32	Subtilase family protein	[more]
AT3G14240.1	4.4e-139	44.17	Subtilase family protein	[more]
AT4G34980.1	6.5e-135	43.55	subtilisin-like serine protease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PRINTS	PR00723	SUBTILISIN	coord: 393..409 score: 55.27 coord: 79..92 score: 37.54
None	No IPR available	GENE3D	3.50.30.30		coord: 212..332 e-value: 4.2E-168 score: 561.9
None	No IPR available	GENE3D	2.60.40.2310		coord: 480..606 e-value: 5.0E-33 score: 115.6
None	No IPR available	PANTHER	PTHR10795:SF662	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 18..608
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 1..463 score: 26.004133
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 240..325 e-value: 6.42963E-29 score: 109.425
None	No IPR available	SUPERFAMILY	52025	PA domain	coord: 247..316
IPR003137	PA domain	PFAM	PF02225	PA	coord: 240..307 e-value: 5.1E-11 score: 42.5
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 14..476 e-value: 4.2E-168 score: 561.9
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 49..467
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 71..435 e-value: 1.9E-41 score: 142.6
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 508..604 e-value: 9.7E-23 score: 80.3
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 18..608
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 394..404
IPR034197	Cucumisin-like catalytic domain	CDD	cd04852	Peptidases_S8_3	coord: 14..429 e-value: 4.54657E-121 score: 358.834

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G03930.1	CSPI03G03930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity