CSPI03G03190 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G03190
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionIRK-interacting protein-like
LocationChr3: 2576198 .. 2577817 (+)
RNA-Seq ExpressionCSPI03G03190
SyntenyCSPI03G03190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCTTCATAGTTCATACATATAAATAACCCCAAAGCTAAGGATAACAGAAAAACCAAAATATTAACTAAAAAATGAAAAATATCACTCCTTCTTCTTCACCATTCTCCTCTGTAAATCTCTCTCTCTTTCTCAATTCTTCTTCAAATCATTCTAAATATGAAACCCATTTGAGTAATAATCTCAATTTTTTGTTTTGCAGATCATTCACAACACTGATAGAACTGAGGAGGAGGAGGAAGAAGATGAGTTCAACAGAGCAACTCCAACTAGTGGTACGGACGCCGGACCAAAGCACCACCCAACACCGCTTCACCTTTACGGCGGTGATGGGGTTCTTCCTAAACGCTCCATCAAGAAACACCCGGACTCAGCGGCTTCCGTCAGCACCGGATCGGTATCCTGCAGTAAGTGCCGACCACATGCTCGAGAAAAGTTCTCTGTAGTTCCGTTGGACAACAATGGCGTCGCTAAACAGTTTTTCTCAATGGCGAGCCCTAATGGAATGCTGAAGTCAATAGTCTCTTCGTTGACAAGAAAAAGCCCTAAACCGATTAATGAAGGAACGGATTTGGCGGCGAGAGAAGAACATTGGAAGATCGCTTTGGGAGAAATTTCGCATAAGTTAATCCAAGCGACGAGGAAAAGAGATGAAGCGATTCTTGAAGCTTCGAGGTTGAAATATTCCATGGCGGAATTGGAGAAGAAACTTAATAAGCTTGAAATCTATTGCCATACTTTGAAATCTGAACTCGATGAATGTGCTTCGAATCCGAGAAAAACTAAACCAGAAGTTCAAAAAAACAGCGATTCGATTTCAGATAAGATTATAGAGAATTTCCTATCGTCAGTTTCTGAATCGCGTTCGTCGGTGAGGCAATTAAGCCGATCATTAGCAATGCAACTCCGTCAAATCGGAGGGAAAATCTACGAGAGGATTCAATTCCTTCTACAATCTCAGGACATCAAAATCCCTCTCTCGAAAAACCTTAAAACTTCACTCATCTTCCATCTCGAAGCTATTCTAAACAGAGCATTCTTCGAGGATTTCGAAACGATAGGGTTTCAGAAGAACTCACCGAATCAGATTTTAAACCCTTCGGATCGGACTGAAGCGAACATCGCGTCGTTTAATCGGCTCCACCGGCTGAGTTGGGAGGAGGTTCTGAGCAAAGGAACGAGGCATTTCAGTGAAGATTTCAGCAGATTTTGTGATCGGAAGATGAGCGACATTGTTGCAATGCTGGAGTGGAACAGAGCTTGGCCGGAGCCACTACTTCAGGCGTTCTTTGCAGCGGCGAAGAGTGTTTGGCTTGTTCATCTTCTTGCTACCGCCGTTCACCCGAGCTTGCCGATATTTAGAGTGGATTCCGGCGTGAGATTCGACGGGGTTTATATGGAGGATATCGCGGGTGAGAAAGCAAGAGAACTTGCTCCGGCGACGGTGAGAATAATGTTGTCGCCGGGATTTTATGTGTTTGATAATTTGATTAAGTGTAAGGTTGTGTGTAGGTATCATATTAATCTTAATCATTAATTTTCTATTCCAATTTACCCAAATTTTATTTTTCTTCCATTTTCCTCATTTTCTCTCATTAATTTCAATAACAAAACCAAGTTTT

mRNA sequence

GCCTTCATAGTTCATACATATAAATAACCCCAAAGCTAAGGATAACAGAAAAACCAAAATATTAACTAAAAAATGAAAAATATCACTCCTTCTTCTTCACCATTCTCCTCTATCATTCACAACACTGATAGAACTGAGGAGGAGGAGGAAGAAGATGAGTTCAACAGAGCAACTCCAACTAGTGGTACGGACGCCGGACCAAAGCACCACCCAACACCGCTTCACCTTTACGGCGGTGATGGGGTTCTTCCTAAACGCTCCATCAAGAAACACCCGGACTCAGCGGCTTCCGTCAGCACCGGATCGGTATCCTGCAGTAAGTGCCGACCACATGCTCGAGAAAAGTTCTCTGTAGTTCCGTTGGACAACAATGGCGTCGCTAAACAGTTTTTCTCAATGGCGAGCCCTAATGGAATGCTGAAGTCAATAGTCTCTTCGTTGACAAGAAAAAGCCCTAAACCGATTAATGAAGGAACGGATTTGGCGGCGAGAGAAGAACATTGGAAGATCGCTTTGGGAGAAATTTCGCATAAGTTAATCCAAGCGACGAGGAAAAGAGATGAAGCGATTCTTGAAGCTTCGAGGTTGAAATATTCCATGGCGGAATTGGAGAAGAAACTTAATAAGCTTGAAATCTATTGCCATACTTTGAAATCTGAACTCGATGAATGTGCTTCGAATCCGAGAAAAACTAAACCAGAAGTTCAAAAAAACAGCGATTCGATTTCAGATAAGATTATAGAGAATTTCCTATCGTCAGTTTCTGAATCGCGTTCGTCGGTGAGGCAATTAAGCCGATCATTAGCAATGCAACTCCGTCAAATCGGAGGGAAAATCTACGAGAGGATTCAATTCCTTCTACAATCTCAGGACATCAAAATCCCTCTCTCGAAAAACCTTAAAACTTCACTCATCTTCCATCTCGAAGCTATTCTAAACAGAGCATTCTTCGAGGATTTCGAAACGATAGGGTTTCAGAAGAACTCACCGAATCAGATTTTAAACCCTTCGGATCGGACTGAAGCGAACATCGCGTCGTTTAATCGGCTCCACCGGCTGAGTTGGGAGGAGGTTCTGAGCAAAGGAACGAGGCATTTCAGTGAAGATTTCAGCAGATTTTGTGATCGGAAGATGAGCGACATTGTTGCAATGCTGGAGTGGAACAGAGCTTGGCCGGAGCCACTACTTCAGGCGTTCTTTGCAGCGGCGAAGAGTGTTTGGCTTGTTCATCTTCTTGCTACCGCCGTTCACCCGAGCTTGCCGATATTTAGAGTGGATTCCGGCGTGAGATTCGACGGGGTTTATATGGAGGATATCGCGGGTGAGAAAGCAAGAGAACTTGCTCCGGCGACGGTGAGAATAATGTTGTCGCCGGGATTTTATGTGTTTGATAATTTGATTAAGTGTAAGGTTGTGTGTAGGTATCATATTAATCTTAATCATTAATTTTCTATTCCAATTTACCCAAATTTTATTTTTCTTCCATTTTCCTCATTTTCTCTCATTAATTTCAATAACAAAACCAAGTTTT

Coding sequence (CDS)

ATGAAAAATATCACTCCTTCTTCTTCACCATTCTCCTCTATCATTCACAACACTGATAGAACTGAGGAGGAGGAGGAAGAAGATGAGTTCAACAGAGCAACTCCAACTAGTGGTACGGACGCCGGACCAAAGCACCACCCAACACCGCTTCACCTTTACGGCGGTGATGGGGTTCTTCCTAAACGCTCCATCAAGAAACACCCGGACTCAGCGGCTTCCGTCAGCACCGGATCGGTATCCTGCAGTAAGTGCCGACCACATGCTCGAGAAAAGTTCTCTGTAGTTCCGTTGGACAACAATGGCGTCGCTAAACAGTTTTTCTCAATGGCGAGCCCTAATGGAATGCTGAAGTCAATAGTCTCTTCGTTGACAAGAAAAAGCCCTAAACCGATTAATGAAGGAACGGATTTGGCGGCGAGAGAAGAACATTGGAAGATCGCTTTGGGAGAAATTTCGCATAAGTTAATCCAAGCGACGAGGAAAAGAGATGAAGCGATTCTTGAAGCTTCGAGGTTGAAATATTCCATGGCGGAATTGGAGAAGAAACTTAATAAGCTTGAAATCTATTGCCATACTTTGAAATCTGAACTCGATGAATGTGCTTCGAATCCGAGAAAAACTAAACCAGAAGTTCAAAAAAACAGCGATTCGATTTCAGATAAGATTATAGAGAATTTCCTATCGTCAGTTTCTGAATCGCGTTCGTCGGTGAGGCAATTAAGCCGATCATTAGCAATGCAACTCCGTCAAATCGGAGGGAAAATCTACGAGAGGATTCAATTCCTTCTACAATCTCAGGACATCAAAATCCCTCTCTCGAAAAACCTTAAAACTTCACTCATCTTCCATCTCGAAGCTATTCTAAACAGAGCATTCTTCGAGGATTTCGAAACGATAGGGTTTCAGAAGAACTCACCGAATCAGATTTTAAACCCTTCGGATCGGACTGAAGCGAACATCGCGTCGTTTAATCGGCTCCACCGGCTGAGTTGGGAGGAGGTTCTGAGCAAAGGAACGAGGCATTTCAGTGAAGATTTCAGCAGATTTTGTGATCGGAAGATGAGCGACATTGTTGCAATGCTGGAGTGGAACAGAGCTTGGCCGGAGCCACTACTTCAGGCGTTCTTTGCAGCGGCGAAGAGTGTTTGGCTTGTTCATCTTCTTGCTACCGCCGTTCACCCGAGCTTGCCGATATTTAGAGTGGATTCCGGCGTGAGATTCGACGGGGTTTATATGGAGGATATCGCGGGTGAGAAAGCAAGAGAACTTGCTCCGGCGACGGTGAGAATAATGTTGTCGCCGGGATTTTATGTGTTTGATAATTTGATTAAGTGTAAGGTTGTGTGTAGGTATCATATTAATCTTAATCATTAA

Protein sequence

MKNITPSSSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLHLYGGDGVLPKRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSIVSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAELEKKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKARELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH*
Homology
BLAST of CSPI03G03190 vs. ExPASy Swiss-Prot
Match: Q9LXU9 (IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 7.8e-61
Identity = 124/311 (39.87%), Postives = 192/311 (61.74%), Query Frame = 0

Query: 150 EISHKLIQATRKRDEAILEASRLKYSMAELEKKLNKLEIYCHTLKSELDEC----ASNPR 209
           ++  +L++A R RD A+ + S +K S+ EL +KL  LE YC  LK  L E     +    
Sbjct: 250 KLKRELMEANRSRDAALTQVSEMKSSLGELSEKLQYLESYCDNLKKALREATEVVSQENS 309

Query: 210 KTKPEVQKNSD-SISDKI-IENFLSSVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLL 269
             +   +KNS+  +S+++ +E FL  VSE+R S++Q  ++L  ++ +    +   I  LL
Sbjct: 310 GGRSSGKKNSEMPVSEEVMVEGFLQIVSEARLSIKQFLKTLVSEIDEEDSTLIGNINTLL 369

Query: 270 QSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASF 329
           Q  ++    +      + +HLEAI++++ ++DFE   FQKN   ++L+P    +AN +SF
Sbjct: 370 QPHNLS--FTSKYSKIIQYHLEAIISQSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSF 429

Query: 330 NRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVW 389
             L  LSW EVL KGT+++S++FSRFCD KMS I+  L W R W E +LQAFF AAK VW
Sbjct: 430 ASLRNLSWNEVLKKGTKYYSDEFSRFCDEKMSLIITTLNWTRPWSEQMLQAFFVAAKCVW 489

Query: 390 LVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKAREL---APATVRIMLSPGFYVFD 449
           L+HLLA + +P+L I RV+    F+  +MED+  ++ R      PA V++M+ PGFYV D
Sbjct: 490 LLHLLAFSFNPALGILRVEENREFESSFMEDMGADRQRSALSRGPARVKVMVMPGFYVLD 549

Query: 450 NLIKCKVVCRY 452
            +++CKV+CRY
Sbjct: 550 RVLRCKVLCRY 558

BLAST of CSPI03G03190 vs. ExPASy Swiss-Prot
Match: F4KGE8 (Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.6e-05
Identity = 49/173 (28.32%), Postives = 69/173 (39.88%), Query Frame = 0

Query: 282 FHLEAILNRAFFEDFETIGF-QKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLS-KGT 341
           F LE+ + R  F+ F+   F    S + ++NP        A F  +  +   E+L    T
Sbjct: 370 FALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFKDMKAMDPMELLGILPT 429

Query: 342 RHFSEDFSRFCDRKMSDIV------------AMLEWNRAWPEPLLQ---AFFAAAKSVWL 401
            H    F +FC +K   I+               E   A   P  Q    F   AK+VWL
Sbjct: 430 CH----FGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPRSQFYGEFLGLAKAVWL 489

Query: 402 VHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAG-EKARELAPATVRIMLSPGF 437
           +HLLA ++ PS   F  + G  F   YME +      R  A   V   + PGF
Sbjct: 490 LHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVVGFPVCPGF 538

BLAST of CSPI03G03190 vs. ExPASy TrEMBL
Match: A0A0A0L1R1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036460 PE=4 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.1e-258
Identity = 456/457 (99.78%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MKNITPSSSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLHLYGGDGVLP 60
           MKNITPSSSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLHLYGGDGVLP
Sbjct: 1   MKNITPSSSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLHLYGGDGVLP 60

Query: 61  KRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSIV 120
           KRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSIV
Sbjct: 61  KRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSIV 120

Query: 121 SSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAELE 180
           SSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSM+ELE
Sbjct: 121 SSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMSELE 180

Query: 181 KKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQL 240
           KKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQL
Sbjct: 181 KKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQL 240

Query: 241 SRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIG 300
           SRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIG
Sbjct: 241 SRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIG 300

Query: 301 FQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAM 360
           FQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAM
Sbjct: 301 FQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAM 360

Query: 361 LEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKA 420
           LEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKA
Sbjct: 361 LEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKA 420

Query: 421 RELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 458
           RELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH
Sbjct: 421 RELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 457

BLAST of CSPI03G03190 vs. ExPASy TrEMBL
Match: A0A1S3BLQ8 (IRK-interacting protein-like OS=Cucumis melo OX=3656 GN=LOC103490937 PE=4 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.4e-246
Identity = 441/458 (96.29%), Postives = 447/458 (97.60%), Query Frame = 0

Query: 1   MKNITPSSSPFSSIIHNTDRTEEEEEE-DEFNRATPTSGTDAGPKHHPTPLHLYGGDGVL 60
           MKNITP SSPF SIIH TDRTEEEEEE DEFN+ATP SGTDAG KHHPTPLHL+G DGVL
Sbjct: 1   MKNITP-SSPFFSIIHKTDRTEEEEEEDDEFNKATPCSGTDAGAKHHPTPLHLHGSDGVL 60

Query: 61  PKRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSI 120
           PKRS KKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSI
Sbjct: 61  PKRSTKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSI 120

Query: 121 VSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAEL 180
           VSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAEL
Sbjct: 121 VSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAEL 180

Query: 181 EKKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQ 240
           EKKLNKLEIYCHTLKSELDECASNPRK K EVQKNSDSISDKIIENFLSSVSESRSSVRQ
Sbjct: 181 EKKLNKLEIYCHTLKSELDECASNPRKPKSEVQKNSDSISDKIIENFLSSVSESRSSVRQ 240

Query: 241 LSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETI 300
           LSRSLAMQLRQIGGK+YERIQFLLQS DIKIPLSKNLKTSLIFHLEAILNRAFFEDFETI
Sbjct: 241 LSRSLAMQLRQIGGKVYERIQFLLQSHDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETI 300

Query: 301 GFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVA 360
           GFQKNSPNQILNPSDRTE+NIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVA
Sbjct: 301 GFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVA 360

Query: 361 MLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEK 420
           MLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEK
Sbjct: 361 MLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEK 420

Query: 421 ARELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 458
           ARELAPATVRIM+SPGFYVFDNLIKCKVVCRYHINLN+
Sbjct: 421 ARELAPATVRIMVSPGFYVFDNLIKCKVVCRYHINLNN 457

BLAST of CSPI03G03190 vs. ExPASy TrEMBL
Match: A0A6J1E8U8 (IRK-interacting protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430947 PE=4 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.3e-212
Identity = 391/469 (83.37%), Postives = 413/469 (88.06%), Query Frame = 0

Query: 1   MKNITPS---------SSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLH 60
           MKNITPS         SSPF SI+ N++RT   EEEDEFNRATP S TDAG KHHPTPLH
Sbjct: 1   MKNITPSPSPPLHHYQSSPFFSIVPNSERT---EEEDEFNRATPGSCTDAGAKHHPTPLH 60

Query: 61  L--YGGDGVLPKRSIKKHPDSAASV-STGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFS 120
           L  +GGD +LPKRS KK  +  ASV   GSVSCSKCRPHAREKFSVVP+D NGVAKQFFS
Sbjct: 61  LHSHGGDEILPKRSTKKRLEPVASVGGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFS 120

Query: 121 MASPNGMLKSIVSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILE 180
           MASPNG+LKSIVSSLT KSPK + EG D A REE WKIA GE+SHKLI+ATRKRDEAILE
Sbjct: 121 MASPNGILKSIVSSLTGKSPKSMKEGADWAVREEQWKIAFGEVSHKLIEATRKRDEAILE 180

Query: 181 ASRLKYSMAELEKKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLS 240
           ASRLKYSMAELEKKLNKLEIYCH+LKSEL +C SNPR    EV  NSDSISDKIIENFLS
Sbjct: 181 ASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLS 240

Query: 241 SVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAIL 300
           SVSESRSSVRQLSRSLAMQL  IGGKIYERIQFLL+S +IKI LSKN KTSLIFHLEAIL
Sbjct: 241 SVSESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAIL 300

Query: 301 NRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSR 360
           NRAFFEDFE+IGFQKNSPNQILNPSDRTE+NIASFNRLHRLSWEEVL KGTRHFSEDFSR
Sbjct: 301 NRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSR 360

Query: 361 FCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFD 420
           FCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSGVRFD
Sbjct: 361 FCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRFD 420

Query: 421 GVYMEDIAGEKARELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 458
           GVYMEDIAGEKARELAPATVRIM++PGFY FD LIKCKV+CRYH NLN+
Sbjct: 421 GVYMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYHTNLNN 466

BLAST of CSPI03G03190 vs. ExPASy TrEMBL
Match: A0A6J1HMP3 (IRK-interacting protein-like OS=Cucurbita maxima OX=3661 GN=LOC111465023 PE=4 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 8.6e-212
Identity = 389/471 (82.59%), Postives = 412/471 (87.47%), Query Frame = 0

Query: 1   MKNITPS-----------SSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTP 60
           MKNITPS           SSPF SI+ N++RT   EEEDEFNRATP+S TD G KHHPTP
Sbjct: 1   MKNITPSSSPSPPLHHYQSSPFFSIVPNSERT---EEEDEFNRATPSSCTDDGAKHHPTP 60

Query: 61  LHL--YGGDGVLPKRSIKKHPDSAASVST-GSVSCSKCRPHAREKFSVVPLDNNGVAKQF 120
           LHL  +GGD +LPKRS KK  +  ASV   GSVSCSKCRPHAREKFSVVP+D NGVAKQF
Sbjct: 61  LHLHSHGGDEILPKRSTKKRLEPVASVGDGGSVSCSKCRPHAREKFSVVPVDYNGVAKQF 120

Query: 121 FSMASPNGMLKSIVSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAI 180
           FSMASPNG+LKSIVSS T KSPK + EG D A REEHWKIA GE+SHKLI+ATRKRDEAI
Sbjct: 121 FSMASPNGILKSIVSSFTGKSPKSMKEGADWAVREEHWKIAFGEVSHKLIEATRKRDEAI 180

Query: 181 LEASRLKYSMAELEKKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENF 240
           LEASRLKYSMAELEKKLNKLEIYCH+LKSEL +C SNPR    EV  NSDSISDKIIENF
Sbjct: 181 LEASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENF 240

Query: 241 LSSVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEA 300
           LSSVSESRSS+RQLSRSLAMQL  IG KIYERIQFLL+S DIKI LSKN KTSLIFHLEA
Sbjct: 241 LSSVSESRSSMRQLSRSLAMQLHHIGDKIYERIQFLLRSHDIKISLSKNSKTSLIFHLEA 300

Query: 301 ILNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDF 360
           ILNRAFFEDFE+IGFQKNSPNQILNPSDRTE+NIASFNRLHRLSWEEVL KGTRHFSEDF
Sbjct: 301 ILNRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDF 360

Query: 361 SRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVR 420
           SRFCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLATAVHPSLPIFRVDSGVR
Sbjct: 361 SRFCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLATAVHPSLPIFRVDSGVR 420

Query: 421 FDGVYMEDIAGEKARELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 458
           FDGVYMEDIAG+KARELAPATVRIM++PGFY FD LIKCKV CRYH NLN+
Sbjct: 421 FDGVYMEDIAGDKARELAPATVRIMVAPGFYAFDGLIKCKVTCRYHTNLNN 468

BLAST of CSPI03G03190 vs. ExPASy TrEMBL
Match: A0A6J1DIL9 (IRK-interacting protein OS=Momordica charantia OX=3673 GN=LOC111020381 PE=4 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 3.4e-200
Identity = 376/465 (80.86%), Postives = 405/465 (87.10%), Query Frame = 0

Query: 1   MKNITPSS--------SP-FSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPL- 60
           MKNITPSS        SP F  II +++RT   EEEDEF     T+ TDAG KHHPTPL 
Sbjct: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERT---EEEDEF-----TNRTDAGAKHHPTPLH 60

Query: 61  -HLYGGDGVLPKRSIKKHPDSAASV--STGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF 120
            H +GGDGVLPKR  KK P+SA  V    G+VSCSKCRPHAREKFSVVPLDNNGVAKQ F
Sbjct: 61  HHSHGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSF 120

Query: 121 SMASPNGMLKSIVSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAIL 180
           SMASPNG+ KSIVSSLTR+SPK +NE  D AAREEHWKIALGEISHKLIQATRKRDEA+L
Sbjct: 121 SMASPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVL 180

Query: 181 EASRLKYSMAELEKKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFL 240
           EASRLKYSMAELE KLNKLEIYCHTLKS LDECA+NPRK+KPEV   SDS+SDKIIENFL
Sbjct: 181 EASRLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEV-LISDSVSDKIIENFL 240

Query: 241 SSVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAI 300
           +SVSESRSSVRQLSRSL MQLR +GGKI+ER+QFLLQ  +IKIP SKN + SL FHLEA+
Sbjct: 241 ASVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEAL 300

Query: 301 LNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFS 360
           LNRAFFEDFE+IGFQKNSPNQILNPSDRTE+NIA+F  LHRL+WEEVLSKGTRHFSE+FS
Sbjct: 301 LNRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFS 360

Query: 361 RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRF 420
           RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSG RF
Sbjct: 361 RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARF 420

Query: 421 DGVYMEDIAGEKARELAPATVRIMLSPGFYVFDNLIKCKVVCRYH 453
           DGVYMEDIAG+   E APATVRIM+SPGFYVFDNLIKCKV+CRYH
Sbjct: 421 DGVYMEDIAGD---ETAPATVRIMVSPGFYVFDNLIKCKVLCRYH 453

BLAST of CSPI03G03190 vs. NCBI nr
Match: XP_004147950.1 (IRK-interacting protein [Cucumis sativus] >KGN55920.1 hypothetical protein Csa_011005 [Cucumis sativus])

HSP 1 Score: 901.0 bits (2327), Expect = 4.3e-258
Identity = 456/457 (99.78%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MKNITPSSSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLHLYGGDGVLP 60
           MKNITPSSSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLHLYGGDGVLP
Sbjct: 1   MKNITPSSSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLHLYGGDGVLP 60

Query: 61  KRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSIV 120
           KRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSIV
Sbjct: 61  KRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSIV 120

Query: 121 SSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAELE 180
           SSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSM+ELE
Sbjct: 121 SSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMSELE 180

Query: 181 KKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQL 240
           KKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQL
Sbjct: 181 KKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQL 240

Query: 241 SRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIG 300
           SRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIG
Sbjct: 241 SRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIG 300

Query: 301 FQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAM 360
           FQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAM
Sbjct: 301 FQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAM 360

Query: 361 LEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKA 420
           LEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKA
Sbjct: 361 LEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKA 420

Query: 421 RELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 458
           RELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH
Sbjct: 421 RELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 457

BLAST of CSPI03G03190 vs. NCBI nr
Match: XP_008448912.1 (PREDICTED: IRK-interacting protein-like [Cucumis melo])

HSP 1 Score: 861.7 bits (2225), Expect = 2.9e-246
Identity = 441/458 (96.29%), Postives = 447/458 (97.60%), Query Frame = 0

Query: 1   MKNITPSSSPFSSIIHNTDRTEEEEEE-DEFNRATPTSGTDAGPKHHPTPLHLYGGDGVL 60
           MKNITP SSPF SIIH TDRTEEEEEE DEFN+ATP SGTDAG KHHPTPLHL+G DGVL
Sbjct: 1   MKNITP-SSPFFSIIHKTDRTEEEEEEDDEFNKATPCSGTDAGAKHHPTPLHLHGSDGVL 60

Query: 61  PKRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSI 120
           PKRS KKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSI
Sbjct: 61  PKRSTKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSI 120

Query: 121 VSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAEL 180
           VSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAEL
Sbjct: 121 VSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAEL 180

Query: 181 EKKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQ 240
           EKKLNKLEIYCHTLKSELDECASNPRK K EVQKNSDSISDKIIENFLSSVSESRSSVRQ
Sbjct: 181 EKKLNKLEIYCHTLKSELDECASNPRKPKSEVQKNSDSISDKIIENFLSSVSESRSSVRQ 240

Query: 241 LSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETI 300
           LSRSLAMQLRQIGGK+YERIQFLLQS DIKIPLSKNLKTSLIFHLEAILNRAFFEDFETI
Sbjct: 241 LSRSLAMQLRQIGGKVYERIQFLLQSHDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETI 300

Query: 301 GFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVA 360
           GFQKNSPNQILNPSDRTE+NIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVA
Sbjct: 301 GFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVA 360

Query: 361 MLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEK 420
           MLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEK
Sbjct: 361 MLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEK 420

Query: 421 ARELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 458
           ARELAPATVRIM+SPGFYVFDNLIKCKVVCRYHINLN+
Sbjct: 421 ARELAPATVRIMVSPGFYVFDNLIKCKVVCRYHINLNN 457

BLAST of CSPI03G03190 vs. NCBI nr
Match: XP_038876802.1 (IRK-interacting protein-like [Benincasa hispida])

HSP 1 Score: 821.2 bits (2120), Expect = 4.3e-234
Identity = 417/457 (91.25%), Postives = 437/457 (95.62%), Query Frame = 0

Query: 1   MKNITPSSSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLHLYGGDGVLP 60
           MKNITPSSSPF S IH T+RT  EEEEDEFNRATP+SGT AG KHHPTPLH +GGDG+LP
Sbjct: 1   MKNITPSSSPFCSFIHKTERT--EEEEDEFNRATPSSGTYAGAKHHPTPLHHHGGDGILP 60

Query: 61  KRSIKKHPDSAASVSTGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSMASPNGMLKSIV 120
           KRS KK P+SAASVS GSVSCSKCRPHAREKFSVVPLDNNGVAKQFFS+ASPNG+LKSIV
Sbjct: 61  KRSTKKRPESAASVSGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSIASPNGILKSIV 120

Query: 121 SSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAELE 180
           SSLTRKSPK +NEGTD AAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAELE
Sbjct: 121 SSLTRKSPKSMNEGTDWAAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMAELE 180

Query: 181 KKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLSSVSESRSSVRQL 240
           KKLNKLEIYCH+LKSELDECASNPRK K EV  NSDSISDKIIENFLSSVSESRSSVRQL
Sbjct: 181 KKLNKLEIYCHSLKSELDECASNPRKPKQEVLTNSDSISDKIIENFLSSVSESRSSVRQL 240

Query: 241 SRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIG 300
           SRSLAMQLRQ+GGKIYERIQFLLQS +IKIPLSKN +TSLIFHLEAILNRAFFEDFE+IG
Sbjct: 241 SRSLAMQLRQMGGKIYERIQFLLQSHEIKIPLSKNPRTSLIFHLEAILNRAFFEDFESIG 300

Query: 301 FQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAM 360
           FQKNSPNQILNPSDRTE+NIASFNRLHRLSWEEVL+KGTRHFSEDFSRFCDRKMSDIVAM
Sbjct: 301 FQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLNKGTRHFSEDFSRFCDRKMSDIVAM 360

Query: 361 LEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKA 420
           LEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAG+KA
Sbjct: 361 LEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGDKA 420

Query: 421 RELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 458
           REL+PA VRIM+SPGFYVFDNLIKCKV+CRYHINLN+
Sbjct: 421 RELSPAAVRIMVSPGFYVFDNLIKCKVLCRYHINLNN 455

BLAST of CSPI03G03190 vs. NCBI nr
Match: XP_023552161.1 (IRK-interacting protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749.2 bits (1933), Expect = 2.1e-212
Identity = 391/471 (83.01%), Postives = 415/471 (88.11%), Query Frame = 0

Query: 1   MKNITPS-----------SSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTP 60
           MKNITPS           SSPF SI+ N++RT   EEEDEFNRATP+S TDAG KHHPTP
Sbjct: 1   MKNITPSSSPSPPLHHYQSSPFFSIVPNSERT---EEEDEFNRATPSSCTDAGAKHHPTP 60

Query: 61  LHL--YGGDGVLPKRSIKKHPDSAASV-STGSVSCSKCRPHAREKFSVVPLDNNGVAKQF 120
           LHL  +GGD +LPKRS KK  +S AS+   GSVSCSKCRPHAREKFSVVP+D NGVAKQF
Sbjct: 61  LHLHSHGGDEILPKRSTKKRLESVASIGGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQF 120

Query: 121 FSMASPNGMLKSIVSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAI 180
           FSMASPNG+LKSIVSSLT KSPK + EG D A REE WKIA GE+SHKLI+ATRKRDEAI
Sbjct: 121 FSMASPNGILKSIVSSLTGKSPKSMKEGGDWAVREEQWKIAFGEVSHKLIEATRKRDEAI 180

Query: 181 LEASRLKYSMAELEKKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENF 240
           LEASRLKYSMAELEKKLNKLEIYCH+LKSEL +C SNPR    EV  NSDSISDKIIENF
Sbjct: 181 LEASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENF 240

Query: 241 LSSVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEA 300
           LSSVSESRSSVRQLSRSLAMQL  IGGKIYERIQFLL+S +IKI LSKN KTSLIFHLEA
Sbjct: 241 LSSVSESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEA 300

Query: 301 ILNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDF 360
           ILNRAFFEDFE+IGFQKNSPNQILNPSDRTE+NIASFNRLHRLSWEEVL KGTRHFSEDF
Sbjct: 301 ILNRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDF 360

Query: 361 SRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVR 420
           SRFCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSGVR
Sbjct: 361 SRFCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVR 420

Query: 421 FDGVYMEDIAGEKARELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 458
           FDGVYMEDIAGEKARELAPATVRIM++PGFY FD LIKCKV+CRYH NLN+
Sbjct: 421 FDGVYMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYHTNLNN 468

BLAST of CSPI03G03190 vs. NCBI nr
Match: XP_022923173.1 (IRK-interacting protein-like [Cucurbita moschata])

HSP 1 Score: 748.0 bits (1930), Expect = 4.7e-212
Identity = 391/469 (83.37%), Postives = 413/469 (88.06%), Query Frame = 0

Query: 1   MKNITPS---------SSPFSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLH 60
           MKNITPS         SSPF SI+ N++RT   EEEDEFNRATP S TDAG KHHPTPLH
Sbjct: 1   MKNITPSPSPPLHHYQSSPFFSIVPNSERT---EEEDEFNRATPGSCTDAGAKHHPTPLH 60

Query: 61  L--YGGDGVLPKRSIKKHPDSAASV-STGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFS 120
           L  +GGD +LPKRS KK  +  ASV   GSVSCSKCRPHAREKFSVVP+D NGVAKQFFS
Sbjct: 61  LHSHGGDEILPKRSTKKRLEPVASVGGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFS 120

Query: 121 MASPNGMLKSIVSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAILE 180
           MASPNG+LKSIVSSLT KSPK + EG D A REE WKIA GE+SHKLI+ATRKRDEAILE
Sbjct: 121 MASPNGILKSIVSSLTGKSPKSMKEGADWAVREEQWKIAFGEVSHKLIEATRKRDEAILE 180

Query: 181 ASRLKYSMAELEKKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFLS 240
           ASRLKYSMAELEKKLNKLEIYCH+LKSEL +C SNPR    EV  NSDSISDKIIENFLS
Sbjct: 181 ASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLS 240

Query: 241 SVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAIL 300
           SVSESRSSVRQLSRSLAMQL  IGGKIYERIQFLL+S +IKI LSKN KTSLIFHLEAIL
Sbjct: 241 SVSESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAIL 300

Query: 301 NRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFSR 360
           NRAFFEDFE+IGFQKNSPNQILNPSDRTE+NIASFNRLHRLSWEEVL KGTRHFSEDFSR
Sbjct: 301 NRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSR 360

Query: 361 FCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFD 420
           FCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSGVRFD
Sbjct: 361 FCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRFD 420

Query: 421 GVYMEDIAGEKARELAPATVRIMLSPGFYVFDNLIKCKVVCRYHINLNH 458
           GVYMEDIAGEKARELAPATVRIM++PGFY FD LIKCKV+CRYH NLN+
Sbjct: 421 GVYMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYHTNLNN 466

BLAST of CSPI03G03190 vs. TAIR 10
Match: AT1G12330.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12900.1); Has 249 Blast hits to 249 proteins in 27 species: Archae - 0; Bacteria - 0; Metazoa - 7; Fungi - 14; Plants - 217; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 457.6 bits (1176), Expect = 1.2e-128
Identity = 266/483 (55.07%), Postives = 331/483 (68.53%), Query Frame = 0

Query: 5   TPSSSPFSSI---IHNTDRTEEEE----EEDEFNRATPTS--GTDAGPKHHP-------- 64
           +PS SP  S+   +H T   E EE    EE   NRATP+S  G+ A P H          
Sbjct: 8   SPSKSPVPSLHQSLHFTPILECEEDDLQEERYKNRATPSSDGGSSATPNHQHHRRTNSNN 67

Query: 65  ------TPLHLYGGDGVLPKRSIKKHPDSAASVSTGSVSCSKCRPH--AREKFSVVPLDN 124
                 TPLH  G      K   +K  D       G+VSC+KCRPH   R+KFSVVPL++
Sbjct: 68  NHQHTLTPLHHNG------KPQTRKRHDDNDEDDGGAVSCNKCRPHHSHRDKFSVVPLES 127

Query: 125 NGVAKQFFSMASPNGMLKSIVSSLTRKSPKPINEGTDL-----------AAREEHWKIAL 184
           +        ++SPN ++KSI  SLTR+SPKP +    L           A+REE W++A+
Sbjct: 128 HNNPS---FISSPNLIIKSIFQSLTRRSPKPSSATAALPPRSSSSSAADASREEQWRLAV 187

Query: 185 GEISHKLIQATRKRDEAILEASRLKYSMAELEKKLNKLEIYCHTLKSELDECASNPRKTK 244
            E+SHKLIQAT+K+++A++EASRLK SMAELEKKLNKLEIYCH LKS LDEC++     K
Sbjct: 188 AELSHKLIQATKKKEDAVIEASRLKSSMAELEKKLNKLEIYCHNLKSGLDECSNK----K 247

Query: 245 PEVQKNSDSISDKIIENFLSSVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLLQSQDI 304
             V    D  +D+II+ FL SVSESRSS+R LSRSLA QLR +GGK+YER+  LLQ  D+
Sbjct: 248 QSVPIRKDGFNDRIIQQFLVSVSESRSSIRALSRSLASQLRTVGGKVYERLSLLLQPFDV 307

Query: 305 KI-PLSKNLKTSLIFHLEAILNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLH 364
           KI   +KN K SLIF+LEAIL+RAFFEDFE  GFQKN   +ILNP DR E+N ASFN L 
Sbjct: 308 KINSFAKNPK-SLIFYLEAILSRAFFEDFEAPGFQKNGSTRILNPIDRCESNYASFNVLM 367

Query: 365 RLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHL 424
            L+W+EVLS+GT+HFSE+FSRFCDRKMSD+V+ML WNRAWPEPLLQAFF A+KSVWLVHL
Sbjct: 368 ELTWDEVLSRGTKHFSEEFSRFCDRKMSDVVSMLSWNRAWPEPLLQAFFGASKSVWLVHL 427

Query: 425 LATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKARELAPATVRIMLSPGFYVFDNLIKCKV 451
           LA +V+P L IFRV+   RFD +YME+  GE+ + L    VR M+ PGFYV+ +++KCKV
Sbjct: 428 LANSVNPGLQIFRVEKDDRFDPIYMEETGGERFKSL----VRAMVQPGFYVYGSVVKCKV 472

BLAST of CSPI03G03190 vs. TAIR 10
Match: AT5G12900.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12330.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 236.1 bits (601), Expect = 5.6e-62
Identity = 124/311 (39.87%), Postives = 192/311 (61.74%), Query Frame = 0

Query: 150 EISHKLIQATRKRDEAILEASRLKYSMAELEKKLNKLEIYCHTLKSELDEC----ASNPR 209
           ++  +L++A R RD A+ + S +K S+ EL +KL  LE YC  LK  L E     +    
Sbjct: 250 KLKRELMEANRSRDAALTQVSEMKSSLGELSEKLQYLESYCDNLKKALREATEVVSQENS 309

Query: 210 KTKPEVQKNSD-SISDKI-IENFLSSVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLL 269
             +   +KNS+  +S+++ +E FL  VSE+R S++Q  ++L  ++ +    +   I  LL
Sbjct: 310 GGRSSGKKNSEMPVSEEVMVEGFLQIVSEARLSIKQFLKTLVSEIDEEDSTLIGNINTLL 369

Query: 270 QSQDIKIPLSKNLKTSLIFHLEAILNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASF 329
           Q  ++    +      + +HLEAI++++ ++DFE   FQKN   ++L+P    +AN +SF
Sbjct: 370 QPHNLS--FTSKYSKIIQYHLEAIISQSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSF 429

Query: 330 NRLHRLSWEEVLSKGTRHFSEDFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVW 389
             L  LSW EVL KGT+++S++FSRFCD KMS I+  L W R W E +LQAFF AAK VW
Sbjct: 430 ASLRNLSWNEVLKKGTKYYSDEFSRFCDEKMSLIITTLNWTRPWSEQMLQAFFVAAKCVW 489

Query: 390 LVHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAGEKAREL---APATVRIMLSPGFYVFD 449
           L+HLLA + +P+L I RV+    F+  +MED+  ++ R      PA V++M+ PGFYV D
Sbjct: 490 LLHLLAFSFNPALGILRVEENREFESSFMEDMGADRQRSALSRGPARVKVMVMPGFYVLD 549

Query: 450 NLIKCKVVCRY 452
            +++CKV+CRY
Sbjct: 550 RVLRCKVLCRY 558

BLAST of CSPI03G03190 vs. TAIR 10
Match: AT3G60680.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 64.3 bits (155), Expect = 2.9e-10
Identity = 76/316 (24.05%), Postives = 139/316 (43.99%), Query Frame = 0

Query: 170 SRLKYSMAELEKKLNKLEIYCHTLKSELDE---CASNPRKTKPEVQKNSDSISDKIIEN- 229
           S L+  + E + KL  LE   + L++E+D       + R    E+QK++  +S ++  N 
Sbjct: 189 SCLESRVQENQSKLRALETVSNRLQAEMDAKDLQVWSLRNKLGEIQKSTSKLSKRLSSNS 248

Query: 230 ----------FLSSVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKN 289
                     F S + ++  + ++ ++ L   + + G      +  + +S   ++  +K 
Sbjct: 249 SLDVLLSVRVFESLLYDAFKATQKFTKILIELMEKAGWD----LDLVAKSVHPEVDYAKE 308

Query: 290 LKTSLIFHLEAILNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVL 349
                 + L + +    F  F+  GF        LN +D  E+  +S +   R   + V 
Sbjct: 309 RHNR--YALLSYVCLGMFRGFDGEGFD-------LNENDYEESERSSVDSSLRELMQHVS 368

Query: 350 SKGTRHFSED----FSRFCDRK---------MSDIVAMLEWNRA----WP--EPLLQAFF 409
           S        D    FSRFCD+K          S I + ++ N A    W       ++F 
Sbjct: 369 SNPMELLDRDKDCAFSRFCDKKYHELIHPNMASSIFSNMDENEAVLSSWRSLSTFYESFV 428

Query: 410 AAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDGVYMEDI---AGEKARELAP--ATVRIM 448
             A S+W +H LA +  P++ IF+V+SGV F  V+ME++     +K   ++P  A V   
Sbjct: 429 TMASSIWTLHKLALSFDPAVEIFQVESGVEFSIVFMENVLKRKQDKKFSMSPTRAKVGFT 488

BLAST of CSPI03G03190 vs. TAIR 10
Match: AT2G45260.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 54.7 bits (130), Expect = 2.3e-07
Identity = 48/184 (26.09%), Postives = 79/184 (42.93%), Query Frame = 0

Query: 282 FHLEAILNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRH 341
           +  E+ + +  F  F+   F  NS +  +  +D T+     F  L  +   + L  GT  
Sbjct: 237 YAFESYICQRMFSGFQQKNFSVNSESAAVMANDDTDTFFRQFLALKDMDPLDAL--GTNP 296

Query: 342 FSEDFSRFCDRKMSDIV------------AMLEWNRAWPEP---LLQAFFAAAKSVWLVH 401
            S +F  FC  K   +V               ++      P     QAF   AKS+W++H
Sbjct: 297 DS-NFGIFCRSKYLLLVHPKMEASFFGNLDQRDYVTGGGHPRTAFYQAFLKLAKSIWILH 356

Query: 402 LLATAVHPSLPIFRVDSGVRFDGVYMEDIAGE---KARELAPATVRIMLSPGFYVFDNLI 448
            LA +  P+  IF+V  G  F   YME +        +E  P  V +M+ PGF++  ++I
Sbjct: 357 RLAYSFDPAAKIFQVKKGSEFSDSYMESVVKNIVVDEKEENP-RVGLMVMPGFWIGGSVI 416

BLAST of CSPI03G03190 vs. TAIR 10
Match: AT5G58960.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 52.4 bits (124), Expect = 1.1e-06
Identity = 49/173 (28.32%), Postives = 69/173 (39.88%), Query Frame = 0

Query: 282 FHLEAILNRAFFEDFETIGF-QKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLS-KGT 341
           F LE+ + R  F+ F+   F    S + ++NP        A F  +  +   E+L    T
Sbjct: 370 FALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFKDMKAMDPMELLGILPT 429

Query: 342 RHFSEDFSRFCDRKMSDIV------------AMLEWNRAWPEPLLQ---AFFAAAKSVWL 401
            H    F +FC +K   I+               E   A   P  Q    F   AK+VWL
Sbjct: 430 CH----FGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPRSQFYGEFLGLAKAVWL 489

Query: 402 VHLLATAVHPSLPIFRVDSGVRFDGVYMEDIAG-EKARELAPATVRIMLSPGF 437
           +HLLA ++ PS   F  + G  F   YME +      R  A   V   + PGF
Sbjct: 490 LHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVVGFPVCPGF 538

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LXU97.8e-6139.87IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1[more]
F4KGE81.6e-0528.32Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0L1R12.1e-25899.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036460 PE=4 SV=1[more]
A0A1S3BLQ81.4e-24696.29IRK-interacting protein-like OS=Cucumis melo OX=3656 GN=LOC103490937 PE=4 SV=1[more]
A0A6J1E8U82.3e-21283.37IRK-interacting protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430947 PE=4 ... [more]
A0A6J1HMP38.6e-21282.59IRK-interacting protein-like OS=Cucurbita maxima OX=3661 GN=LOC111465023 PE=4 SV... [more]
A0A6J1DIL93.4e-20080.86IRK-interacting protein OS=Momordica charantia OX=3673 GN=LOC111020381 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147950.14.3e-25899.78IRK-interacting protein [Cucumis sativus] >KGN55920.1 hypothetical protein Csa_0... [more]
XP_008448912.12.9e-24696.29PREDICTED: IRK-interacting protein-like [Cucumis melo][more]
XP_038876802.14.3e-23491.25IRK-interacting protein-like [Benincasa hispida][more]
XP_023552161.12.1e-21283.01IRK-interacting protein-like [Cucurbita pepo subsp. pepo][more]
XP_022923173.14.7e-21283.37IRK-interacting protein-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G12330.11.2e-12855.07unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplas... [more]
AT5G12900.15.6e-6239.87unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G60680.12.9e-1024.05Plant protein of unknown function (DUF641) [more]
AT2G45260.12.3e-0726.09Plant protein of unknown function (DUF641) [more]
AT5G58960.11.1e-0628.32Plant protein of unknown function (DUF641) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 162..196
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..82
NoneNo IPR availablePANTHERPTHR31029:SF4CYCLIN-DEPENDENT KINASE-LIKE PROTEINcoord: 6..452
IPR042316IRK-interacting protein-likePANTHERPTHR31029CYCLIN-DEPENDENT KINASE-LIKE PROTEINcoord: 6..452

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G03190.1CSPI03G03190.1mRNA