CSPI03G02770 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G02770
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: (RGF1), (RGF2), (RGF3), (RGF4), (RGF5), (RGF6), (RGF7), (RGF8) and (RGF9).
Location	Chr3: 2158828 .. 2160136 (+)
RNA-Seq Expression	CSPI03G02770
Synteny	CSPI03G02770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCTAATTCCATCTCTCATTATTCCAAACAAAACCCTCTTCTCTTTCACCTTTTCTCTCTTTTTCCTTTCTCATGGCTATGCCTTATTTCTTTCTCATTCTCTTATTCTTCTCATCTACTTTGCATGCATGTGACGCTAGTCGTCTTCGATTCTTCCAGAGTTCGAATTTTGTTTCCAGGATTAATGAACTGCATTCGATTTCTCAAGGGATTTGGAGTACAAATCATGATCAGGAGGGAAGTTTCGACAAAAACAACAATGACAACATTCTTCATAATAAGGAGAATATTGTTGGAATTGAAATGTTAAAGGTATACTATACATATATACTTCATCTCCCATCTTGTGCTTTCTAAAGATGAGCTTGTGATTTACTTCATAATTACTAAGCTAGCTATATGAGAGGGGGAGTTTTTAGATATGTTGGACATTTTCATTTAAACATATCTACATCTTGATAAAACTCAATATATCAAAATATATATGAGGCGATAAAAATATATTTTTCCTCTAAATAATGCAATAAATATGTCAACCAAACTATACATGCATACCTTATCTTATTTAAAAATTCAATCAAATGGTTTTTGGTCGAAATATAATGTGTAATTATCGATATCAAATATATTTGATTTTATAAAAAAACCAACATAATATCCTTTTATCATTATTTCTATTTATATTTGTACAAGCTTTAAAATCTCATAATTTTCATCGACATCAACATTTAATTCTCGAGTCAAGATAATAATAATCATGAATATAGACACCATTTAAGTTCTTTGGTACAGGACAATAATAATTTCATTAATACAGACAAAGGCATATGAATGATAAATACATGAAAAGGGGAATTATTTGGAAATATAGTGAGTGAGTGATTAATGAATTAACTTTGGTATTTTAATTTATCAGGGAATGAAGAAAGATGGAAGCAGATTGGTAATGGAGTTTCCAATGAAAGAAAAAGTGATGAATTCTAAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCACAGGAAGCCTCCTATTCACAATGAAAAACCCTAGATATATGTATAGATATATTGTAATATTTCTCAAATTTGCCATCAAATATTTAAATATGTTTAAAAAATAAAAGTCTTTTTCTTTTTTAATTTTTCTATATATTGGAATAAAGAAAAAAAATAGAGTATAAATAACTATGTGAGGATTTATCTTTGATTTTATTTTAAAATTTATATATGCAAACGACTTTAATTTGTATTTTGTATTCAAGGGTTTTTGTTATTTATATAATTTGAGTGA

mRNA sequence

CCCTAATTCCATCTCTCATTATTCCAAACAAAACCCTCTTCTCTTTCACCTTTTCTCTCTTTTTCCTTTCTCATGGCTATGCCTTATTTCTTTCTCATTCTCTTATTCTTCTCATCTACTTTGCATGCATGTGACGCTAGTCGTCTTCGATTCTTCCAGAGTTCGAATTTTGTTTCCAGGATTAATGAACTGCATTCGATTTCTCAAGGGATTTGGAGTACAAATCATGATCAGGAGGGAAGTTTCGACAAAAACAACAATGACAACATTCTTCATAATAAGGAGAATATTGTTGGAATTGAAATGTTAAAGGGAATGAAGAAAGATGGAAGCAGATTGGTAATGGAGTTTCCAATGAAAGAAAAAGTGATGAATTCTAAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCACAGGAAGCCTCCTATTCACAATGAAAAACCCTAGATATATGTATAGATATATTGTAATATTTCTCAAATTTGCCATCAAATATTTAAATATGTTTAAAAAATAAAAGTCTTTTTCTTTTTTAATTTTTCTATATATTGGAATAAAGAAAAAAAATAGAGTATAAATAACTATGTGAGGATTTATCTTTGATTTTATTTTAAAATTTATATATGCAAACGACTTTAATTTGTATTTTGTATTCAAGGGTTTTTGTTATTTATATAATTTGAGTGA

Coding sequence (CDS)

ATGGCTATGCCTTATTTCTTTCTCATTCTCTTATTCTTCTCATCTACTTTGCATGCATGTGACGCTAGTCGTCTTCGATTCTTCCAGAGTTCGAATTTTGTTTCCAGGATTAATGAACTGCATTCGATTTCTCAAGGGATTTGGAGTACAAATCATGATCAGGAGGGAAGTTTCGACAAAAACAACAATGACAACATTCTTCATAATAAGGAGAATATTGTTGGAATTGAAATGTTAAAGGGAATGAAGAAAGATGGAAGCAGATTGGTAATGGAGTTTCCAATGAAAGAAAAAGTGATGAATTCTAAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCACAGGAAGCCTCCTATTCACAATGAAAAACCCTAG

Protein sequence

MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSISQGIWSTNHDQEGSFDKNNNDNILHNKENIVGIEMLKGMKKDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPHRKPPIHNEKP*

Homology

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BLAST of CSPI03G02770 vs. ExPASy Swiss-Prot
Match: Q93VK8 (Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.9e-05
Identity = 20/24 (83.33%), Postives = 22/24 (91.67%), Query Frame = 0


Query: 106 EVGEDMVVMDYAQPHRKPPIHNEK 130
           E GE++VVMDY QPHRKPPIHNEK
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

[Close]

BLAST of CSPI03G02770 vs. ExPASy TrEMBL
Match: A0A0A0L298 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.2e-64
Identity = 126/130 (96.92%), Postives = 129/130 (99.23%), Query Frame = 0


Query: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSISQGIWSTNHDQEGSFDK 60
           MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHS+SQGIWS NHDQEGSFDK
Sbjct: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60

Query: 61  NNNDNILHNKENIVGIEMLKGMKKDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120
           NNNDNILHNKENI+GIEMLKGMK+DGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH
Sbjct: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120

Query: 121 RKPPIHNEKP 131
           RKPPIHNEKP
Sbjct: 121 RKPPIHNEKP 130

[Close]

BLAST of CSPI03G02770 vs. ExPASy TrEMBL
Match: A0A6J1DKL1 (uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021396 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 9.9e-20
Identity = 76/135 (56.30%), Postives = 89/135 (65.93%), Query Frame = 0


Query: 1   MAMPYFFLILLFFSSTLHACDA-SRLRFFQSSNFVSRINELHSISQGIWSTNHDQEGSFD 60
           MA P    ILL   STLHACDA  RL+ F+ SN VSR+  L  I Q       DQ    +
Sbjct: 1   MATPSLLFILLL--STLHACDACHRLQLFEYSNPVSRLGLLAPIPQ---EFRTDQP---E 60

Query: 61  KNNNDNILHNKENIV--GIEMLKGMKKDGSRLVMEFPM--KEKVMNSKENEVGEDMVVMD 120
           ++ N NI+H   + V  GI +LKGMKK   RL MEF M  KEK+M+ +ENEVGEDMVVMD
Sbjct: 61  RSLNKNIIHKVSSSVEIGIGILKGMKKH-KRLAMEFSMKEKEKLMSYEENEVGEDMVVMD 120

Query: 121 YAQPHRKPPIHNEKP 131
           YAQPHRKPPIHN+KP
Sbjct: 121 YAQPHRKPPIHNKKP 126

[Close]

BLAST of CSPI03G02770 vs. ExPASy TrEMBL
Match: A0A6J1KZM9 (uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 3.5e-17
Identity = 75/142 (52.82%), Postives = 89/142 (62.68%), Query Frame = 0


Query: 1   MAMP-YFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSISQGIWSTNHDQ-EGSF 60
           M MP   FL LLF S+ LHACDA  L+   SS   S +     IS G      DQ + S 
Sbjct: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCM-----ISPGF---RMDQLKRSL 60

Query: 61  DKNNNDNILHNKENIVGIEMLKGMKKDGSRLVMEFPMK----------EKVMNSKENEVG 120
           DK+ + N + + EN VGIE+LKGMKK   RL ME P++          +KV+NS+ENEVG
Sbjct: 61  DKSKS-NEVSSVEN-VGIEILKGMKK-LRRLAMELPVRDRDRDKDKDIDKVVNSEENEVG 120

Query: 121 EDMVVMDYAQPHRKPPIHNEKP 131
           EDMVVMDYAQPHRKPPIHN KP
Sbjct: 121 EDMVVMDYAQPHRKPPIHNRKP 131

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BLAST of CSPI03G02770 vs. ExPASy TrEMBL
Match: A0A438K320 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_009086 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 6.2e-06
Identity = 42/124 (33.87%), Postives = 67/124 (54.03%), Query Frame = 0


Query: 8   LILLFFSSTLHACDASRLRFFQSSNFVSRINELHSISQGIWSTNHDQEGSFDKNNNDNIL 67
           L+LL  S ++HAC+A  L      N      E+H +S+ +   +   + S       + +
Sbjct: 5   LLLLLLSLSMHACNARHLGVIGKEN-----REVHLLSKVVEEADQVHKISLPPRLIPS-M 64

Query: 68  HNKENIVGIEMLKGMKKDGSRLVMEFPMK-EKVMNSKENEVGEDMVVMDYAQPHRKPPIH 127
              E +   ++L G K+    ++   P   E+ ++SKE ++ ED+VVMDYAQPHRKPPIH
Sbjct: 65  ETAEKLQPQQILPGWKRHSRLMLGSAPHDVEETVDSKEGDIVEDIVVMDYAQPHRKPPIH 122

Query: 128 NEKP 131
           N+ P
Sbjct: 125 NKDP 122

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BLAST of CSPI03G02770 vs. ExPASy TrEMBL
Match: A0A2C9WK84 (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G079300 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 6.2e-06
Identity = 30/65 (46.15%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 66  ILHNKENIVGIEMLKGMKKDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPHRKPPI 125
           I+ ++ N+     ++G+KK            +K + S EN++ ED+VVMDYAQPHRKPPI
Sbjct: 112 IVSSQRNLQQTAKIEGLKKQAR------SYNKKALESGENDIVEDVVVMDYAQPHRKPPI 170

Query: 126 HNEKP 131
           HNEKP
Sbjct: 172 HNEKP 170

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BLAST of CSPI03G02770 vs. NCBI nr
Match: XP_011650407.1 (uncharacterized protein LOC105434783 [Cucumis sativus] >KGN55873.1 hypothetical protein Csa_010787 [Cucumis sativus])

HSP 1 Score: 254.6 bits (649), Expect = 4.6e-64
Identity = 126/130 (96.92%), Postives = 129/130 (99.23%), Query Frame = 0


Query: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSISQGIWSTNHDQEGSFDK 60
           MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHS+SQGIWS NHDQEGSFDK
Sbjct: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60

Query: 61  NNNDNILHNKENIVGIEMLKGMKKDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120
           NNNDNILHNKENI+GIEMLKGMK+DGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH
Sbjct: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120

Query: 121 RKPPIHNEKP 131
           RKPPIHNEKP
Sbjct: 121 RKPPIHNEKP 130

[Close]

BLAST of CSPI03G02770 vs. NCBI nr
Match: XP_038905997.1 (uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida])

HSP 1 Score: 183.3 bits (464), Expect = 1.3e-42
Identity = 100/128 (78.12%), Postives = 111/128 (86.72%), Query Frame = 0


Query: 3   MPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSISQGIWSTNHDQEGSFDKNN 62
           MPY FLILL FSSTLHAC+A  LRFF+SS+FVSRI E HSISQGIW+     E SF+++N
Sbjct: 1   MPYLFLILLIFSSTLHACEARCLRFFESSSFVSRI-EPHSISQGIWA--DQPERSFEESN 60

Query: 63  NDNILHNKENIVGIEMLKGMKKDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPHRK 122
           +D + + KE IVGIEMLKGMKK GSRLVMEFPMKEKV+NSKENEVGEDMVVMDYAQPHRK
Sbjct: 61  SDKLSY-KEIIVGIEMLKGMKKHGSRLVMEFPMKEKVLNSKENEVGEDMVVMDYAQPHRK 120

Query: 123 PPIHNEKP 131
           PPIHNEKP
Sbjct: 121 PPIHNEKP 124

[Close]

BLAST of CSPI03G02770 vs. NCBI nr
Match: XP_038905996.1 (uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida])

HSP 1 Score: 178.7 bits (452), Expect = 3.2e-41
Identity = 100/129 (77.52%), Postives = 111/129 (86.05%), Query Frame = 0


Query: 3   MPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSISQGIWSTNHDQEGSFDKNN 62
           MPY FLILL FSSTLHAC+A  LRFF+SS+FVSRI E HSISQGIW+     E SF+++N
Sbjct: 1   MPYLFLILLIFSSTLHACEARCLRFFESSSFVSRI-EPHSISQGIWA--DQPERSFEESN 60

Query: 63  NDNILHNKENIVGIEMLK-GMKKDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPHR 122
           +D + + KE IVGIEMLK GMKK GSRLVMEFPMKEKV+NSKENEVGEDMVVMDYAQPHR
Sbjct: 61  SDKLSY-KEIIVGIEMLKQGMKKHGSRLVMEFPMKEKVLNSKENEVGEDMVVMDYAQPHR 120

Query: 123 KPPIHNEKP 131
           KPPIHNEKP
Sbjct: 121 KPPIHNEKP 125

[Close]

BLAST of CSPI03G02770 vs. NCBI nr
Match: XP_022154042.1 (uncharacterized protein LOC111021396 [Momordica charantia])

HSP 1 Score: 106.3 bits (264), Expect = 2.0e-19
Identity = 76/135 (56.30%), Postives = 89/135 (65.93%), Query Frame = 0


Query: 1   MAMPYFFLILLFFSSTLHACDA-SRLRFFQSSNFVSRINELHSISQGIWSTNHDQEGSFD 60
           MA P    ILL   STLHACDA  RL+ F+ SN VSR+  L  I Q       DQ    +
Sbjct: 1   MATPSLLFILLL--STLHACDACHRLQLFEYSNPVSRLGLLAPIPQ---EFRTDQP---E 60

Query: 61  KNNNDNILHNKENIV--GIEMLKGMKKDGSRLVMEFPM--KEKVMNSKENEVGEDMVVMD 120
           ++ N NI+H   + V  GI +LKGMKK   RL MEF M  KEK+M+ +ENEVGEDMVVMD
Sbjct: 61  RSLNKNIIHKVSSSVEIGIGILKGMKKH-KRLAMEFSMKEKEKLMSYEENEVGEDMVVMD 120

Query: 121 YAQPHRKPPIHNEKP 131
           YAQPHRKPPIHN+KP
Sbjct: 121 YAQPHRKPPIHNKKP 126

[Close]

BLAST of CSPI03G02770 vs. NCBI nr
Match: XP_023540680.1 (uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 104.0 bits (258), Expect = 1.0e-18
Identity = 74/139 (53.24%), Postives = 90/139 (64.75%), Query Frame = 0


Query: 1   MAMP-YFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSISQGIWSTNHDQEGSFD 60
           M MP  FFL LLF S+ LHACDA  L+   SS   S +     IS G + TN  +    +
Sbjct: 1   MPMPTSFFLALLFLSTFLHACDARLLKLCHSSRLESCL-----ISPG-FRTNQLERSLDE 60

Query: 61  KNNNDNILHNKENIVGIEMLKGMKKDGSRLVMEFPMK--------EKVMNSKENEVGEDM 120
             +ND  + + EN VGIE+LKGMKK   RL ME P++        +KV+NS+ENEVGEDM
Sbjct: 61  SKSND--VSSMEN-VGIEILKGMKK-LRRLAMELPVRDRDRDKDIDKVVNSEENEVGEDM 120

Query: 121 VVMDYAQPHRKPPIHNEKP 131
           VVMDYAQPHRKPPIHN+KP
Sbjct: 121 VVMDYAQPHRKPPIHNQKP 129

[Close]

BLAST of CSPI03G02770 vs. TAIR 10
Match: AT4G16515.1 (Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: At5g60810 (RGF1), At1g13620 (RGF2), At2g04025 (RGF3), At3g30350 (RGF4), At5g51451 (RGF5), At4g16515 (RGF6), At3g02240 (RGF7), At2g03830 (RGF8) and At5g64770 (RGF9). )

HSP 1 Score: 49.3 bits (116), Expect = 2.8e-06
Identity = 20/24 (83.33%), Postives = 22/24 (91.67%), Query Frame = 0


Query: 106 EVGEDMVVMDYAQPHRKPPIHNEK 130
           E GE++VVMDY QPHRKPPIHNEK
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

The following BLAST results are available for this feature:

BLAST of CSPI03G02770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VK8	3.9e-05	83.33	Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1	[more]

BLAST of CSPI03G02770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L298	2.2e-64	96.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1	[more]
A0A6J1DKL1	9.9e-20	56.30	uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A6J1KZM9	3.5e-17	52.82	uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521...	[more]
A0A438K320	6.2e-06	33.87	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_009086 PE=4 SV=1	[more]
A0A2C9WK84	6.2e-06	46.15	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_01G079300 PE=4 SV=...	[more]

BLAST of CSPI03G02770 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650407.1	4.6e-64	96.92	uncharacterized protein LOC105434783 [Cucumis sativus] >KGN55873.1 hypothetical ...	[more]
XP_038905997.1	1.3e-42	78.13	uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida]	[more]
XP_038905996.1	3.2e-41	77.52	uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida]	[more]
XP_022154042.1	2.0e-19	56.30	uncharacterized protein LOC111021396 [Momordica charantia]	[more]
XP_023540680.1	1.0e-18	53.24	uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI03G02770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16515.1	2.8e-06	83.33	Encodes a root meristem growth factor (RGF). Belongs to a family of functionall...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34961	TRANSMEMBRANE PROTEIN	coord: 3..130
None	No IPR available	PANTHER	PTHR34961:SF7	SUBFAMILY NOT NAMED	coord: 3..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G02770.1	CSPI03G02770.1	mRNA