CSPI03G02620 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G02620
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionWAT1-related protein
LocationChr3: 2004293 .. 2006869 (+)
RNA-Seq ExpressionCSPI03G02620
SyntenyCSPI03G02620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCATGGGAGAGAAAAAGAGACTTTCTTTGAGGAGATTTGTTGATTTCTTGTTGCCATAAGAACTTTTTTTTTCTTTGTTAAAAAGGAAAAGATGAAGAGAGAAACAATGGAAGATTTTGCAATAATGAGTGGGTTGGTTGGAGTGCAGTTTGTATATGCAGGAAACTCTGTTTTTATGAGTTTTCTTATGTCTCTAGGGATTGATCCTTTAACCCTTGTTATCTTCTCTACCTTCTCTACTTTTCTCATTGTCTCTCCCGTTGCTGTTTATTTCGAAAGGTTTGTTTTGTCTCTTTCTCATGAATTGTTAGATGATATAATATTAAACTTATCTTCGTTTAGTATTAGAGTAAAAGTCTAAGAGGTTTCATGTTGGATGATATAATATTTAAGTTTTTGGGATAATTAGTGATTTAAAATGGTATTAGAGAAGGAGGGTAGGTGATATGTCAAAACCTATACAATCTTATTCCCTTTCGGTTTAATATTGAATATTAAGTTTACATTCGATTCTATCACTTATCAACTTAAGGTTTTGGGTCATCCTGCGATTTTAGTGAAAAAAATTTACTTTTTTCCGTCCTTTTTTAGGTAGAAGAATTTGGATGATGAAACAGAAAAGTTCATGGTTTTGTGCAGGTATAACTGGCCAAAGAAACTTAGTCTAAAGTTGATCCTTCAGCTTGTTCTCATATCTTTTGGAGGGTAAGAAATTTAAATGGCTAAACCCACCAATGAAAATATGGATGCCAACGTTTGGTTATTTTAGCTATGAAAACATGCATGGATTATTGTTAGAGGAAAGTGAAGAGAAAAGTGATTTGGGATACTGACATAATGTGTTTGTTTGTAGGGTTACGTTGTTTCAGTCCCTTTTACTGAAAGGAATACAGCTAACTTCGCCGACTTTAGCTACGGCGATGCCGAATCTCGCCCCTGGACTCATCTTTGCCATTGCTTGGATCTTTAGGTACACTGAAAGCTACTTTTCTGTTATGGAAACATTGTAGAAAGTAGGAAGAAAGAACATTCATGGGATTCATTTTCTTCACTTTTTTTTTTTTTTGATAAATATGGATCAAAATTGTTCACTTTCGACCATTTTTCGTGTTCGATAAGAGACATTTTATCATGAATGCAGATTAGAAAAAGTTCAGCTAAGTTGCATCTATAGCAAGATCAAGATTTTAGGTACATTACTATGTGTGATAGGCGCATTCACCATGAGCATAATGCACAGTGCTCCATCGGATGTAGCTGGAAAAAATAGTATCCCACAACAATCACCTCTGAGTGAGCAACTGTTTGATGAACAGAAGATCCTCGGTTGTGCGTATCTCTTCGTGGCGATCATTATTTTATCTTGCATAGTTGTTCTGCAGGTATCTCAATGGTTATTATATATAAACAATACCTAGTATGTTAAGAATGCCTTAAATCTATTATCCAATACTTCGAACAACCAAGCATGCAGGTCTTGCTTTTAGCCCTAAGATTTAAAGAAGTGTAGTATATGAACTCTTCAACTCTATTGGCAATTTTGAATCTAAATATGGTATTTATCTTTAATAATAAATAGAACTCTATGGTCAATTGACAGGCAGCGACACTAGGCGATTTCCCGGCACCAATGTCGTTGTGTGCGATAACATCCCTCATCGGAGTGCTCTTAACCATAGGTGTACAGATAATTCAATATCACAGAATTGATATTGCATGGCCACTTCTAATCGGTGTTAAAGAATTGGCTGCCTATGTCATTCTAGTAAGCAGCATTTAATCCTATGCTCTTTTGAGATAATTTTTTCTACATATTGTGTGCATATTGATGTATGCTTTTAAAAATCTTGTTGACGAAAAGGGCGGAACGATAAGTGGCGCTTGCGTGAGCTTCAACGGGTGGGCGATGAAGAAACGAGGGCCAGTTTTGGTGTCAATGTTTAGTCCGATAGGAACAGTTTGTTCACTCGTTCTCTCAGTTATCACCTTAGGAGAATCCATCAACATTGGAAGGTACATTACTACCATTTCTTTCTCATGATCGTCGTAAACCAACACGTTATTCGAGCATGATCTTCATATTTGTTTCGCAATATGCAGCCTTGCTGGTATGTTCATGATGTTCACCGGCCTCTACTTCGTCCTATGGGCGAAAGGGAAGGAACGTTATGTGAACCGAAACCATCTCGAGAGTGAGTTCGACGTTGACAAGCCTCTTTTAAGTTGAATTATTTCATTTTGAAATACATAGGTTCTGTTTTGTCACATTAGGCATTAGAAACACAAACACTTAGAAGGACTATTTTTCTATAGTGGGGTTGGGATTGGGATTGTTATTTAGAACAGTTCCAACATTTTGAACATTTAACTTTTGCAGAAAGTTGAGGAGCTTCAGTCTTTGGAATGTTTGTTTTGTTTGGGAAAATTCTGTCTCCTCTATGCTTGAGTTTTGTAAAGACAGAAGATTCACTTCAGCAACTCAACACATCATATTCATATCTCTCTCTCTCTTTTCTCTTTTGTAGATTATATCAGTTTGGATTTTGTTTATGTGCTTAAGTTTGTGATGGAACAGAG

mRNA sequence

GCATGGGAGAGAAAAAGAGACTTTCTTTGAGGAGATTTGTTGATTTCTTGTTGCCATAAGAACTTTTTTTTTCTTTGTTAAAAAGGAAAAGATGAAGAGAGAAACAATGGAAGATTTTGCAATAATGAGTGGGTTGGTTGGAGTGCAGTTTGTATATGCAGGAAACTCTGTTTTTATGAGTTTTCTTATGTCTCTAGGGATTGATCCTTTAACCCTTGTTATCTTCTCTACCTTCTCTACTTTTCTCATTGTCTCTCCCGTTGCTGTTTATTTCGAAAGGTATAACTGGCCAAAGAAACTTAGTCTAAAGTTGATCCTTCAGCTTGTTCTCATATCTTTTGGAGGGGTTACGTTGTTTCAGTCCCTTTTACTGAAAGGAATACAGCTAACTTCGCCGACTTTAGCTACGGCGATGCCGAATCTCGCCCCTGGACTCATCTTTGCCATTGCTTGGATCTTTAGATTAGAAAAAGTTCAGCTAAGTTGCATCTATAGCAAGATCAAGATTTTAGGTACATTACTATGTGTGATAGGCGCATTCACCATGAGCATAATGCACAGTGCTCCATCGGATGTAGCTGGAAAAAATAGTATCCCACAACAATCACCTCTGAGTGAGCAACTGTTTGATGAACAGAAGATCCTCGGTTGTGCGTATCTCTTCGTGGCGATCATTATTTTATCTTGCATAGTTGTTCTGCAGGCAGCGACACTAGGCGATTTCCCGGCACCAATGTCGTTGTGTGCGATAACATCCCTCATCGGAGTGCTCTTAACCATAGGTGTACAGATAATTCAATATCACAGAATTGATATTGCATGGCCACTTCTAATCGGTGTTAAAGAATTGGCTGCCTATGTCATTCTAGGCGGAACGATAAGTGGCGCTTGCGTGAGCTTCAACGGGTGGGCGATGAAGAAACGAGGGCCAGTTTTGGTGTCAATGTTTAGTCCGATAGGAACAGTTTGTTCACTCGTTCTCTCAGTTATCACCTTAGGAGAATCCATCAACATTGGAAGCCTTGCTGGTATGTTCATGATGTTCACCGGCCTCTACTTCGTCCTATGGGCGAAAGGGAAGGAACGTTATGTGAACCGAAACCATCTCGAGAAAAGTTGAGGAGCTTCAGTCTTTGGAATGTTTGTTTTGTTTGGGAAAATTCTGTCTCCTCTATGCTTGAGTTTTGTAAAGACAGAAGATTCACTTCAGCAACTCAACACATCATATTCATATCTCTCTCTCTCTTTTCTCTTTTGTAGATTATATCAGTTTGGATTTTGTTTATGTGCTTAAGTTTGTGATGGAACAGAG

Coding sequence (CDS)

ATGAAGAGAGAAACAATGGAAGATTTTGCAATAATGAGTGGGTTGGTTGGAGTGCAGTTTGTATATGCAGGAAACTCTGTTTTTATGAGTTTTCTTATGTCTCTAGGGATTGATCCTTTAACCCTTGTTATCTTCTCTACCTTCTCTACTTTTCTCATTGTCTCTCCCGTTGCTGTTTATTTCGAAAGGTATAACTGGCCAAAGAAACTTAGTCTAAAGTTGATCCTTCAGCTTGTTCTCATATCTTTTGGAGGGGTTACGTTGTTTCAGTCCCTTTTACTGAAAGGAATACAGCTAACTTCGCCGACTTTAGCTACGGCGATGCCGAATCTCGCCCCTGGACTCATCTTTGCCATTGCTTGGATCTTTAGATTAGAAAAAGTTCAGCTAAGTTGCATCTATAGCAAGATCAAGATTTTAGGTACATTACTATGTGTGATAGGCGCATTCACCATGAGCATAATGCACAGTGCTCCATCGGATGTAGCTGGAAAAAATAGTATCCCACAACAATCACCTCTGAGTGAGCAACTGTTTGATGAACAGAAGATCCTCGGTTGTGCGTATCTCTTCGTGGCGATCATTATTTTATCTTGCATAGTTGTTCTGCAGGCAGCGACACTAGGCGATTTCCCGGCACCAATGTCGTTGTGTGCGATAACATCCCTCATCGGAGTGCTCTTAACCATAGGTGTACAGATAATTCAATATCACAGAATTGATATTGCATGGCCACTTCTAATCGGTGTTAAAGAATTGGCTGCCTATGTCATTCTAGGCGGAACGATAAGTGGCGCTTGCGTGAGCTTCAACGGGTGGGCGATGAAGAAACGAGGGCCAGTTTTGGTGTCAATGTTTAGTCCGATAGGAACAGTTTGTTCACTCGTTCTCTCAGTTATCACCTTAGGAGAATCCATCAACATTGGAAGCCTTGCTGGTATGTTCATGATGTTCACCGGCCTCTACTTCGTCCTATGGGCGAAAGGGAAGGAACGTTATGTGAACCGAAACCATCTCGAGAAAAGTTGA

Protein sequence

MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLEKS*
Homology
BLAST of CSPI03G02620 vs. ExPASy Swiss-Prot
Match: Q9FGL0 (WAT1-related protein At5g47470 OS=Arabidopsis thaliana OX=3702 GN=At5g47470 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.5e-104
Identity = 201/333 (60.36%), Postives = 253/333 (75.98%), Query Frame = 0

Query: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60
           MK E +E+  I+ GLV VQFVYAGNS+ MS+LMSLG+ P T+VIFSTF+TF+I+SP A+ 
Sbjct: 20  MKTEMIEEMVIVGGLVMVQFVYAGNSLLMSYLMSLGLGPFTIVIFSTFATFIILSPFAIL 79

Query: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120
           FER  WP +LSL+LI +LVLISF GVTLFQSL L+GI+LTSP +ATAMPNLAPGLIF IA
Sbjct: 80  FERKQWPNELSLRLIGKLVLISFAGVTLFQSLFLEGIRLTSPAMATAMPNLAPGLIFFIA 139

Query: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180
           WI  LEK+ L C+YSK+KILGTLLCV GA  MS+MHS  + ++ K      +P+   +FD
Sbjct: 140 WIVGLEKMNLKCVYSKLKILGTLLCVFGALAMSVMHS--TSISHKEE--DDTPIF--VFD 199

Query: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240
             K++GC YL  A+ +LS  VVLQA+TL +FPAP+SL AIT+L+GVL+T  V ++Q  + 
Sbjct: 200 RDKVVGCIYLLGAVFVLSTNVVLQASTLAEFPAPISLSAITALLGVLITTVVLLLQNRKT 259

Query: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300
            +    LI    L  Y +L G +SGACVSFNGWAMKKRGPV VSMFSP  TV S+  +V+
Sbjct: 260 KVLASSLISFGNLVGYSVLAGAVSGACVSFNGWAMKKRGPVFVSMFSPFATVISVAFAVL 319

Query: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERY 334
           TLGES+++GS+ GM +MF GLY VLWAKGKE +
Sbjct: 320 TLGESVSLGSVGGMVLMFVGLYLVLWAKGKEGF 346

BLAST of CSPI03G02620 vs. ExPASy Swiss-Prot
Match: F4JMI7 (WAT1-related protein At4g16620 OS=Arabidopsis thaliana OX=3702 GN=At4g16620 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.5e-85
Identity = 168/331 (50.76%), Postives = 235/331 (71.00%), Query Frame = 0

Query: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60
           MKRET+ +  I+ GL G Q +YAGNS  +S L+SLGIDPL +VI  TF++ L+++P+A  
Sbjct: 2   MKRETLIEAGIIGGLAGAQVIYAGNSELLSQLLSLGIDPLLIVILCTFASVLLITPLAFL 61

Query: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120
            ER  WP+ LS KL ++LVL++  GVTLFQ L L+G++ TS ++ATAMPNL P  IF IA
Sbjct: 62  LERKLWPRSLSFKLKIKLVLVALAGVTLFQGLFLEGMKHTSASMATAMPNLCPAFIFVIA 121

Query: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180
           W   +EKV+LSC+YS++K+ GT+LCV+GA  MS+MHS  + ++   +IP      E + D
Sbjct: 122 WAAGMEKVKLSCMYSRVKMGGTVLCVMGALIMSLMHSTTATLSSVKTIPIVP--DEVVVD 181

Query: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240
           + KILGC YL +AI  LS  +VLQA+ L +FPAP+S+ ++ SL+G + T+ +Q      +
Sbjct: 182 KDKILGCLYLLLAICGLSSSIVLQASILAEFPAPISMFSMVSLMGGITTVALQYALKGSM 241

Query: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300
           ++    +IG+  L  Y ILGG +SG  +SFN W +K++GPV+VS+FSPI TV  +V+S  
Sbjct: 242 EMGSASVIGLGHLVGYAILGGLVSGGGLSFNAWVIKRKGPVIVSLFSPIATVVCVVVSAF 301

Query: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKE 332
           T+ ES N+GS AGM +MF GLYFVLWAKGKE
Sbjct: 302 TMEESFNLGSFAGMALMFGGLYFVLWAKGKE 330

BLAST of CSPI03G02620 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.6e-32
Identity = 106/320 (33.12%), Postives = 165/320 (51.56%), Query Frame = 0

Query: 15  LVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYNWPKKLSLKL 74
           +V +QF YAG S+   F ++ G+ P  L  +      + ++P A + +R   P K++L +
Sbjct: 12  VVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRP-KMTLSI 71

Query: 75  ILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRLEKVQLSCIY 134
             +++L+     T+ Q+L   G++ TS T   AM N+ P   F +AWIFRLEKV +  I+
Sbjct: 72  FFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIH 131

Query: 135 SKIKILGTLLCVIGAFTMSIMHS--APSDVAGKNSIPQQSPLSEQLFDEQKILGCAYLFV 194
           S+ KILGT++ V GA  M+++     P   A  + I Q S  +    D  K  G + + +
Sbjct: 132 SQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTK--GASLIAI 191

Query: 195 AIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWPLLIGVKE 254
             I  +  + LQA TL  +P  +SL A    +G + +  V +        AW + +  K 
Sbjct: 192 GCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSKL 251

Query: 255 LAAYVILGGTI-SGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGESINIGSL 314
           LAA  + GG I SG      G  MK RGPV V+ F+P+  V   +L  I L E + +G +
Sbjct: 252 LAA--VYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 311

Query: 315 AGMFMMFTGLYFVLWAKGKE 332
            G  ++  GLY VLW K K+
Sbjct: 312 LGAIVIVLGLYSVLWGKSKD 326

BLAST of CSPI03G02620 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 6.3e-31
Identity = 98/334 (29.34%), Postives = 164/334 (49.10%), Query Frame = 0

Query: 2   KRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYF 61
           KR+ ME       +V +Q   AG  +    +++ G+    LV++      ++++P A YF
Sbjct: 6   KRDCMEKARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYF 65

Query: 62  ERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAW 121
           ++   P K++L +  ++ L+      + Q+L   G++ T+ T ATAM N+ P + F +A+
Sbjct: 66  DKKVRP-KMTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAY 125

Query: 122 IFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDE 181
           IF LE+V+L CI S  K++GTL  V GA  M+++     D+     +   +     +   
Sbjct: 126 IFGLERVKLRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTDI--H 185

Query: 182 QKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRID 241
             I G   + +     +C ++LQA TL  +PA +SL A   L+G +    V ++      
Sbjct: 186 SAIKGAVLVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNP 245

Query: 242 IAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVIT 301
            AW +    K L A    G   S       G  MK RGPV V+ FSP+  +   ++S I 
Sbjct: 246 SAWAIGWDTKLLTA-TYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTII 305

Query: 302 LGESINIGSLAGMFMMFTGLYFVLWAKGKERYVN 336
             E + +G + G  ++  GLY V+W KGK+   N
Sbjct: 306 FAEQMYLGRVLGAVVICAGLYLVIWGKGKDYKYN 335

BLAST of CSPI03G02620 vs. ExPASy Swiss-Prot
Match: Q9FNA5 (WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 6.3e-31
Identity = 98/330 (29.70%), Postives = 169/330 (51.21%), Query Frame = 0

Query: 15  LVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYNWPKKLSLKL 74
           +V +Q +YA  S+     ++ G+ P  LV +       +++P A+  ER   P KL+ K+
Sbjct: 12  IVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRP-KLTFKI 71

Query: 75  ILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRLEKVQLSCIY 134
           +LQ+ ++S     + Q+L   G++LT+ T  +A+ N  P + F +A +F+LEKV +   +
Sbjct: 72  LLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERRH 131

Query: 135 SKIKILGTLLCVIGAFTMSIMHSA------PSDVAGKNS------IPQQSPLSEQLFDEQ 194
           S+ K++GT++ + GA  M+ +          S+  G N       IP+Q+ ++       
Sbjct: 132 SQAKLVGTMVAIGGAMLMTFVKGNVIELPWTSNSRGLNGHTHAMRIPKQADIAR------ 191

Query: 195 KILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDI 254
              G   L  +    SC ++LQA  L  + A +SL A+  ++G+L    + +I   +   
Sbjct: 192 ---GSIMLVASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMS 251

Query: 255 AWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITL 314
            W +   V  LA+  I GG +SG      GWA K+RGPV VS F+P+  V   +LS    
Sbjct: 252 VWKINPDVTLLAS--IYGGLVSGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVF 311

Query: 315 GESINIGSLAGMFMMFTGLYFVLWAKGKER 333
            E + +G + G  ++  G+Y VLW K K++
Sbjct: 312 LEKVYVGRVIGSVVIVIGIYLVLWGKSKDK 329

BLAST of CSPI03G02620 vs. ExPASy TrEMBL
Match: A0A0A0L5H5 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_3G019970 PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 8.5e-180
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 6   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYN 65
           MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYN
Sbjct: 1   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYN 60

Query: 66  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 125
           WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL
Sbjct: 61  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 120

Query: 126 EKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKIL 185
           EKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKIL
Sbjct: 121 EKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKIL 180

Query: 186 GCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWP 245
           GCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWP
Sbjct: 181 GCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWP 240

Query: 246 LLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGES 305
           LLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGES
Sbjct: 241 LLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGES 300

Query: 306 INIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLEKS 343
           INIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLEKS
Sbjct: 301 INIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLEKS 337

BLAST of CSPI03G02620 vs. ExPASy TrEMBL
Match: A0A1S3BKN0 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103490881 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 321/340 (94.41%), Postives = 333/340 (97.94%), Query Frame = 0

Query: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60
           MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY
Sbjct: 12  MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 71

Query: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120
           FERY+WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA
Sbjct: 72  FERYSWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 131

Query: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180
           WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMS+MHSA SD AGK+S+P+QSPLSE +FD
Sbjct: 132 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFD 191

Query: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240
           EQKILGCAYLF AIIILS +VVLQA+TLGDFPAPMSLCAITSLIGVLLTIGVQI+QYHRI
Sbjct: 192 EQKILGCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRI 251

Query: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300
           DIAWPLLI VKELAAYVI+GGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSV+
Sbjct: 252 DIAWPLLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVV 311

Query: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLE 341
           TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYV+ NHLE
Sbjct: 312 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLE 351

BLAST of CSPI03G02620 vs. ExPASy TrEMBL
Match: A0A5D3CP19 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00410 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.4e-169
Identity = 316/335 (94.33%), Postives = 328/335 (97.91%), Query Frame = 0

Query: 6   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYN 65
           MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERY+
Sbjct: 1   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYS 60

Query: 66  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 125
           WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL
Sbjct: 61  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 120

Query: 126 EKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKIL 185
           EKVQLSCIYSKIKILGTLLCVIGAFTMS+MHSA SD AGK+S+P+QSPLSE +FDEQKIL
Sbjct: 121 EKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFDEQKIL 180

Query: 186 GCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWP 245
           GCAYLF AIIILS +VVLQA+TLGDFPAPMSLCAITSLIGVLLTIGVQI+QYHRIDIAWP
Sbjct: 181 GCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRIDIAWP 240

Query: 246 LLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGES 305
           LLI VKELAAYVI+GGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSV+TLGES
Sbjct: 241 LLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVVTLGES 300

Query: 306 INIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLE 341
           INIGSLAGMFMMFTGLYFVLWAKGKERYV+ NHLE
Sbjct: 301 INIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLE 335

BLAST of CSPI03G02620 vs. ExPASy TrEMBL
Match: A0A6J1DKW5 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111021397 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.6e-138
Identity = 261/337 (77.45%), Postives = 292/337 (86.65%), Query Frame = 0

Query: 6   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYN 65
           MED AIM GLVGVQFVYAGNSV MSF MS+GIDPLTLVIFSTFSTFLIVSP+AVYFER  
Sbjct: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60

Query: 66  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 125
           WP KL+LKLI QL+LISFGGVTLFQSL LKGIQLTSP+LATAMPNLAPGLIF IAWIFRL
Sbjct: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120

Query: 126 EKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKIL 185
           EKV+LSCIYSKIKILGTLLCV+GAFTM+IMHSA       ++I       +Q+ D QKIL
Sbjct: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASK---VDSTILPLFDEQDQILDRQKIL 180

Query: 186 GCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWP 245
           GC YLF A+++LS IVVLQAATLGDFPAPMSLCAITSLIGV+ TIGVQI++Y  ++I WP
Sbjct: 181 GCLYLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWP 240

Query: 246 LLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGES 305
             + VKE+AAY ++GG++SGACVSFNGWAMKKRGPVLVSMFSPIGTV SL+LS+ TLGES
Sbjct: 241 WFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGES 300

Query: 306 INIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLEKS 343
           IN+GSLAGMF+MFTGLYFVLWAK KE Y  RN  EK+
Sbjct: 301 INVGSLAGMFLMFTGLYFVLWAKRKESYGTRNGPEKT 334

BLAST of CSPI03G02620 vs. ExPASy TrEMBL
Match: A0A6J1DJ66 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111021397 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 2.3e-137
Identity = 259/332 (78.01%), Postives = 289/332 (87.05%), Query Frame = 0

Query: 6   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYN 65
           MED AIM GLVGVQFVYAGNSV MSF MS+GIDPLTLVIFSTFSTFLIVSP+AVYFER  
Sbjct: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60

Query: 66  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 125
           WP KL+LKLI QL+LISFGGVTLFQSL LKGIQLTSP+LATAMPNLAPGLIF IAWIFRL
Sbjct: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120

Query: 126 EKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKIL 185
           EKV+LSCIYSKIKILGTLLCV+GAFTM+IMHSA       ++I       +Q+ D QKIL
Sbjct: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASK---VDSTILPLFDEQDQILDRQKIL 180

Query: 186 GCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWP 245
           GC YLF A+++LS IVVLQAATLGDFPAPMSLCAITSLIGV+ TIGVQI++Y  ++I WP
Sbjct: 181 GCLYLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWP 240

Query: 246 LLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGES 305
             + VKE+AAY ++GG++SGACVSFNGWAMKKRGPVLVSMFSPIGTV SL+LS+ TLGES
Sbjct: 241 WFVSVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGES 300

Query: 306 INIGSLAGMFMMFTGLYFVLWAKGKERYVNRN 338
           IN+GSLAGMF+MFTGLYFVLWAK KE Y  RN
Sbjct: 301 INVGSLAGMFLMFTGLYFVLWAKRKESYGTRN 329

BLAST of CSPI03G02620 vs. NCBI nr
Match: XP_011650397.1 (WAT1-related protein At5g47470 isoform X2 [Cucumis sativus] >KAE8650056.1 hypothetical protein Csa_009979 [Cucumis sativus])

HSP 1 Score: 649.4 bits (1674), Expect = 1.7e-182
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60
           MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY
Sbjct: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60

Query: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120
           FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA
Sbjct: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120

Query: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180
           WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD
Sbjct: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180

Query: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240
           EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI
Sbjct: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240

Query: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300
           DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI
Sbjct: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300

Query: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLEKS 343
           TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLEKS
Sbjct: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLEKS 342

BLAST of CSPI03G02620 vs. NCBI nr
Match: XP_004147210.1 (WAT1-related protein At5g47470 isoform X1 [Cucumis sativus])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0

Query: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60
           MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY
Sbjct: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60

Query: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120
           FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA
Sbjct: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120

Query: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180
           WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD
Sbjct: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180

Query: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240
           EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI
Sbjct: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240

Query: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300
           DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI
Sbjct: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300

Query: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLE 341
           TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLE
Sbjct: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLE 340

BLAST of CSPI03G02620 vs. NCBI nr
Match: XP_008448840.2 (PREDICTED: WAT1-related protein At5g47470 [Cucumis melo])

HSP 1 Score: 615.5 bits (1586), Expect = 2.7e-172
Identity = 321/340 (94.41%), Postives = 333/340 (97.94%), Query Frame = 0

Query: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60
           MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY
Sbjct: 12  MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 71

Query: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120
           FERY+WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA
Sbjct: 72  FERYSWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 131

Query: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180
           WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMS+MHSA SD AGK+S+P+QSPLSE +FD
Sbjct: 132 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFD 191

Query: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240
           EQKILGCAYLF AIIILS +VVLQA+TLGDFPAPMSLCAITSLIGVLLTIGVQI+QYHRI
Sbjct: 192 EQKILGCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRI 251

Query: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300
           DIAWPLLI VKELAAYVI+GGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSV+
Sbjct: 252 DIAWPLLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVV 311

Query: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLE 341
           TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYV+ NHLE
Sbjct: 312 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLE 351

BLAST of CSPI03G02620 vs. NCBI nr
Match: KAA0041079.1 (WAT1-related protein [Cucumis melo var. makuwa] >TYK12029.1 WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 605.5 bits (1560), Expect = 2.8e-169
Identity = 316/335 (94.33%), Postives = 328/335 (97.91%), Query Frame = 0

Query: 6   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYN 65
           MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERY+
Sbjct: 1   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYS 60

Query: 66  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 125
           WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL
Sbjct: 61  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 120

Query: 126 EKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKIL 185
           EKVQLSCIYSKIKILGTLLCVIGAFTMS+MHSA SD AGK+S+P+QSPLSE +FDEQKIL
Sbjct: 121 EKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFDEQKIL 180

Query: 186 GCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWP 245
           GCAYLF AIIILS +VVLQA+TLGDFPAPMSLCAITSLIGVLLTIGVQI+QYHRIDIAWP
Sbjct: 181 GCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRIDIAWP 240

Query: 246 LLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGES 305
           LLI VKELAAYVI+GGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSV+TLGES
Sbjct: 241 LLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVVTLGES 300

Query: 306 INIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLE 341
           INIGSLAGMFMMFTGLYFVLWAKGKERYV+ NHLE
Sbjct: 301 INIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLE 335

BLAST of CSPI03G02620 vs. NCBI nr
Match: XP_038906245.1 (WAT1-related protein At5g47470 [Benincasa hispida])

HSP 1 Score: 575.1 bits (1481), Expect = 4.1e-160
Identity = 299/337 (88.72%), Postives = 319/337 (94.66%), Query Frame = 0

Query: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60
           MKRETME+FAIMSGLVGVQFVYAGNSVFMSFLMSLGI+PLTLVIFSTFSTFLIVSP+AVY
Sbjct: 1   MKRETMENFAIMSGLVGVQFVYAGNSVFMSFLMSLGINPLTLVIFSTFSTFLIVSPIAVY 60

Query: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120
           FER++WPKKLSLKLILQL+LISFGGVTLFQSL LKGIQLTSP LATAMPNL PGLIFAIA
Sbjct: 61  FERHDWPKKLSLKLILQLLLISFGGVTLFQSLFLKGIQLTSPALATAMPNLTPGLIFAIA 120

Query: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180
           WIFRLEKVQLSCIYSKIKILGT LCVIGAFTMSIMHSA SD A K++IP+QSP SEQ+F+
Sbjct: 121 WIFRLEKVQLSCIYSKIKILGTFLCVIGAFTMSIMHSASSDAAAKDNIPRQSPPSEQVFN 180

Query: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240
           EQKILGC YLF AII+LS IVVLQA+TLGDFPAPMSLCAITSLIGVL+TI VQI+QY ++
Sbjct: 181 EQKILGCVYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVLITIVVQIVQYQKL 240

Query: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300
           DI WPLLI  KELAAY +LGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSL+LSV 
Sbjct: 241 DIGWPLLISGKELAAYFVLGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVF 300

Query: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRN 338
           TLGESINIGSLAGMF+MFTGLYFVLWAKGKERYVNRN
Sbjct: 301 TLGESINIGSLAGMFLMFTGLYFVLWAKGKERYVNRN 337

BLAST of CSPI03G02620 vs. TAIR 10
Match: AT5G47470.1 (Nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 378.3 bits (970), Expect = 6.8e-105
Identity = 201/333 (60.36%), Postives = 253/333 (75.98%), Query Frame = 0

Query: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60
           MK E +E+  I+ GLV VQFVYAGNS+ MS+LMSLG+ P T+VIFSTF+TF+I+SP A+ 
Sbjct: 20  MKTEMIEEMVIVGGLVMVQFVYAGNSLLMSYLMSLGLGPFTIVIFSTFATFIILSPFAIL 79

Query: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120
           FER  WP +LSL+LI +LVLISF GVTLFQSL L+GI+LTSP +ATAMPNLAPGLIF IA
Sbjct: 80  FERKQWPNELSLRLIGKLVLISFAGVTLFQSLFLEGIRLTSPAMATAMPNLAPGLIFFIA 139

Query: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180
           WI  LEK+ L C+YSK+KILGTLLCV GA  MS+MHS  + ++ K      +P+   +FD
Sbjct: 140 WIVGLEKMNLKCVYSKLKILGTLLCVFGALAMSVMHS--TSISHKEE--DDTPIF--VFD 199

Query: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240
             K++GC YL  A+ +LS  VVLQA+TL +FPAP+SL AIT+L+GVL+T  V ++Q  + 
Sbjct: 200 RDKVVGCIYLLGAVFVLSTNVVLQASTLAEFPAPISLSAITALLGVLITTVVLLLQNRKT 259

Query: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300
            +    LI    L  Y +L G +SGACVSFNGWAMKKRGPV VSMFSP  TV S+  +V+
Sbjct: 260 KVLASSLISFGNLVGYSVLAGAVSGACVSFNGWAMKKRGPVFVSMFSPFATVISVAFAVL 319

Query: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERY 334
           TLGES+++GS+ GM +MF GLY VLWAKGKE +
Sbjct: 320 TLGESVSLGSVGGMVLMFVGLYLVLWAKGKEGF 346

BLAST of CSPI03G02620 vs. TAIR 10
Match: AT4G16620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.1e-86
Identity = 168/331 (50.76%), Postives = 235/331 (71.00%), Query Frame = 0

Query: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60
           MKRET+ +  I+ GL G Q +YAGNS  +S L+SLGIDPL +VI  TF++ L+++P+A  
Sbjct: 2   MKRETLIEAGIIGGLAGAQVIYAGNSELLSQLLSLGIDPLLIVILCTFASVLLITPLAFL 61

Query: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120
            ER  WP+ LS KL ++LVL++  GVTLFQ L L+G++ TS ++ATAMPNL P  IF IA
Sbjct: 62  LERKLWPRSLSFKLKIKLVLVALAGVTLFQGLFLEGMKHTSASMATAMPNLCPAFIFVIA 121

Query: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180
           W   +EKV+LSC+YS++K+ GT+LCV+GA  MS+MHS  + ++   +IP      E + D
Sbjct: 122 WAAGMEKVKLSCMYSRVKMGGTVLCVMGALIMSLMHSTTATLSSVKTIPIVP--DEVVVD 181

Query: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240
           + KILGC YL +AI  LS  +VLQA+ L +FPAP+S+ ++ SL+G + T+ +Q      +
Sbjct: 182 KDKILGCLYLLLAICGLSSSIVLQASILAEFPAPISMFSMVSLMGGITTVALQYALKGSM 241

Query: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300
           ++    +IG+  L  Y ILGG +SG  +SFN W +K++GPV+VS+FSPI TV  +V+S  
Sbjct: 242 EMGSASVIGLGHLVGYAILGGLVSGGGLSFNAWVIKRKGPVIVSLFSPIATVVCVVVSAF 301

Query: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKE 332
           T+ ES N+GS AGM +MF GLYFVLWAKGKE
Sbjct: 302 TMEESFNLGSFAGMALMFGGLYFVLWAKGKE 330

BLAST of CSPI03G02620 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.8e-33
Identity = 106/320 (33.12%), Postives = 165/320 (51.56%), Query Frame = 0

Query: 15  LVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYNWPKKLSLKL 74
           +V +QF YAG S+   F ++ G+ P  L  +      + ++P A + +R   P K++L +
Sbjct: 12  VVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRP-KMTLSI 71

Query: 75  ILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRLEKVQLSCIY 134
             +++L+     T+ Q+L   G++ TS T   AM N+ P   F +AWIFRLEKV +  I+
Sbjct: 72  FFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIH 131

Query: 135 SKIKILGTLLCVIGAFTMSIMHS--APSDVAGKNSIPQQSPLSEQLFDEQKILGCAYLFV 194
           S+ KILGT++ V GA  M+++     P   A  + I Q S  +    D  K  G + + +
Sbjct: 132 SQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTK--GASLIAI 191

Query: 195 AIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWPLLIGVKE 254
             I  +  + LQA TL  +P  +SL A    +G + +  V +        AW + +  K 
Sbjct: 192 GCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSKL 251

Query: 255 LAAYVILGGTI-SGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGESINIGSL 314
           LAA  + GG I SG      G  MK RGPV V+ F+P+  V   +L  I L E + +G +
Sbjct: 252 LAA--VYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 311

Query: 315 AGMFMMFTGLYFVLWAKGKE 332
            G  ++  GLY VLW K K+
Sbjct: 312 LGAIVIVLGLYSVLWGKSKD 326

BLAST of CSPI03G02620 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 136.3 bits (342), Expect = 4.5e-32
Identity = 98/334 (29.34%), Postives = 164/334 (49.10%), Query Frame = 0

Query: 2   KRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYF 61
           KR+ ME       +V +Q   AG  +    +++ G+    LV++      ++++P A YF
Sbjct: 6   KRDCMEKARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYF 65

Query: 62  ERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAW 121
           ++   P K++L +  ++ L+      + Q+L   G++ T+ T ATAM N+ P + F +A+
Sbjct: 66  DKKVRP-KMTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAY 125

Query: 122 IFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDE 181
           IF LE+V+L CI S  K++GTL  V GA  M+++     D+     +   +     +   
Sbjct: 126 IFGLERVKLRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTDI--H 185

Query: 182 QKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRID 241
             I G   + +     +C ++LQA TL  +PA +SL A   L+G +    V ++      
Sbjct: 186 SAIKGAVLVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNP 245

Query: 242 IAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVIT 301
            AW +    K L A    G   S       G  MK RGPV V+ FSP+  +   ++S I 
Sbjct: 246 SAWAIGWDTKLLTA-TYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTII 305

Query: 302 LGESINIGSLAGMFMMFTGLYFVLWAKGKERYVN 336
             E + +G + G  ++  GLY V+W KGK+   N
Sbjct: 306 FAEQMYLGRVLGAVVICAGLYLVIWGKGKDYKYN 335

BLAST of CSPI03G02620 vs. TAIR 10
Match: AT5G13670.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 136.3 bits (342), Expect = 4.5e-32
Identity = 98/330 (29.70%), Postives = 169/330 (51.21%), Query Frame = 0

Query: 15  LVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYNWPKKLSLKL 74
           +V +Q +YA  S+     ++ G+ P  LV +       +++P A+  ER   P KL+ K+
Sbjct: 12  IVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRP-KLTFKI 71

Query: 75  ILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRLEKVQLSCIY 134
           +LQ+ ++S     + Q+L   G++LT+ T  +A+ N  P + F +A +F+LEKV +   +
Sbjct: 72  LLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVTIERRH 131

Query: 135 SKIKILGTLLCVIGAFTMSIMHSA------PSDVAGKNS------IPQQSPLSEQLFDEQ 194
           S+ K++GT++ + GA  M+ +          S+  G N       IP+Q+ ++       
Sbjct: 132 SQAKLVGTMVAIGGAMLMTFVKGNVIELPWTSNSRGLNGHTHAMRIPKQADIAR------ 191

Query: 195 KILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDI 254
              G   L  +    SC ++LQA  L  + A +SL A+  ++G+L    + +I   +   
Sbjct: 192 ---GSIMLVASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMS 251

Query: 255 AWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITL 314
            W +   V  LA+  I GG +SG      GWA K+RGPV VS F+P+  V   +LS    
Sbjct: 252 VWKINPDVTLLAS--IYGGLVSGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVF 311

Query: 315 GESINIGSLAGMFMMFTGLYFVLWAKGKER 333
            E + +G + G  ++  G+Y VLW K K++
Sbjct: 312 LEKVYVGRVIGSVVIVIGIYLVLWGKSKDK 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FGL09.5e-10460.36WAT1-related protein At5g47470 OS=Arabidopsis thaliana OX=3702 GN=At5g47470 PE=3... [more]
F4JMI71.5e-8550.76WAT1-related protein At4g16620 OS=Arabidopsis thaliana OX=3702 GN=At4g16620 PE=2... [more]
O806382.6e-3233.13WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2... [more]
Q9ZUS16.3e-3129.34WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2... [more]
Q9FNA56.3e-3129.70WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0L5H58.5e-180100.00WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_3G019970 PE=3 SV=1[more]
A0A1S3BKN01.3e-17294.41WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103490881 PE=3 SV=1[more]
A0A5D3CP191.4e-16994.33WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold101... [more]
A0A6J1DKW53.6e-13877.45WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111021397 PE=3 SV=1[more]
A0A6J1DJ662.3e-13778.01WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111021397 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011650397.11.7e-182100.00WAT1-related protein At5g47470 isoform X2 [Cucumis sativus] >KAE8650056.1 hypoth... [more]
XP_004147210.12.4e-181100.00WAT1-related protein At5g47470 isoform X1 [Cucumis sativus][more]
XP_008448840.22.7e-17294.41PREDICTED: WAT1-related protein At5g47470 [Cucumis melo][more]
KAA0041079.12.8e-16994.33WAT1-related protein [Cucumis melo var. makuwa] >TYK12029.1 WAT1-related protein... [more]
XP_038906245.14.1e-16088.72WAT1-related protein At5g47470 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G47470.16.8e-10560.36Nodulin MtN21 /EamA-like transporter family protein [more]
AT4G16620.11.1e-8650.76nodulin MtN21 /EamA-like transporter family protein [more]
AT2G39510.11.8e-3333.13nodulin MtN21 /EamA-like transporter family protein [more]
AT2G37460.14.5e-3229.34nodulin MtN21 /EamA-like transporter family protein [more]
AT5G13670.14.5e-3229.70nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 16..153
e-value: 2.0E-8
score: 34.5
coord: 185..325
e-value: 2.4E-7
score: 31.0
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 10..338
NoneNo IPR availablePANTHERPTHR31218:SF14OS11G0588100 PROTEINcoord: 10..338
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 51..153
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 250..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G02620.1CSPI03G02620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity