CSPI03G02050 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G02050
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	protein N-lysine methyltransferase METTL21A-like
Location	Chr3: 1507293 .. 1507958 (-)
RNA-Seq Expression	CSPI03G02050
Synteny	CSPI03G02050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTTAAAGAGTTCCAAATCAGTGATCGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGAAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTCGTCCTAGCCAAGTGGATGGCAACTCAGTGCAACCTATTCGACTTCGACTTCCGGCAGAAGAACGTAATCGAGCTTGGCGCTGGGACTGGACTTCCTGGTTTGACAGCAGCTTTACTTGGTGCTAATCGTGTTCTACTTACCGATGTAGAGCCATTGCTTCCTGGGTTGTTGGAGAATGTAGATGCTAATGGAGTTGGCGATCGAGTGGAAGTAAGGGAACTCGTGTGGGGCTCGAACGACTTGGCAAGTCAAGCAAATGAGTTGGGGGAGTTTGACCTGATTTTGATGTCAGATTTGTTCTACGACTCGGAGGAAATGCCCCATCTAGCACAAGTACTAAAGATCATCTCTGGGACAGATACCAAAATATGGGCAGCATCGGAGATCCGACCATGGACTCTTGAATGCATTACAGAGTTGATAAATCAAGGATTCAAGATCACAGAGTCCCCAGTTCAACATGATGAATCTCATGAGGAAGACAACCATAAAATTTTGGACTCGTTTTCTATATTCCATCTCAGACAAGAAGAAAGAAACCTTTAA

mRNA sequence

ATGGTTGTTAAAGAGTTCCAAATCAGTGATCGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGAAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTCGTCCTAGCCAAGTGGATGGCAACTCAGTGCAACCTATTCGACTTCGACTTCCGGCAGAAGAACGTAATCGAGCTTGGCGCTGGGACTGGACTTCCTGGTTTGACAGCAGCTTTACTTGGTGCTAATCGTGTTCTACTTACCGATGTAGAGCCATTGCTTCCTGGGTTGTTGGAGAATGTAGATGCTAATGGAGTTGGCGATCGAGTGGAAGTAAGGGAACTCGTGTGGGGCTCGAACGACTTGGCAAGTCAAGCAAATGAGTTGGGGGAGTTTGACCTGATTTTGATGTCAGATTTGTTCTACGACTCGGAGGAAATGCCCCATCTAGCACAAGTACTAAAGATCATCTCTGGGACAGATACCAAAATATGGGCAGCATCGGAGATCCGACCATGGACTCTTGAATGCATTACAGAGTTGATAAATCAAGGATTCAAGATCACAGAGTCCCCAGTTCAACATGATGAATCTCATGAGGAAGACAACCATAAAATTTTGGACTCGTTTTCTATATTCCATCTCAGACAAGAAGAAAGAAACCTTTAA

Coding sequence (CDS)

ATGGTTGTTAAAGAGTTCCAAATCAGTGATCGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGAAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTCGTCCTAGCCAAGTGGATGGCAACTCAGTGCAACCTATTCGACTTCGACTTCCGGCAGAAGAACGTAATCGAGCTTGGCGCTGGGACTGGACTTCCTGGTTTGACAGCAGCTTTACTTGGTGCTAATCGTGTTCTACTTACCGATGTAGAGCCATTGCTTCCTGGGTTGTTGGAGAATGTAGATGCTAATGGAGTTGGCGATCGAGTGGAAGTAAGGGAACTCGTGTGGGGCTCGAACGACTTGGCAAGTCAAGCAAATGAGTTGGGGGAGTTTGACCTGATTTTGATGTCAGATTTGTTCTACGACTCGGAGGAAATGCCCCATCTAGCACAAGTACTAAAGATCATCTCTGGGACAGATACCAAAATATGGGCAGCATCGGAGATCCGACCATGGACTCTTGAATGCATTACAGAGTTGATAAATCAAGGATTCAAGATCACAGAGTCCCCAGTTCAACATGATGAATCTCATGAGGAAGACAACCATAAAATTTTGGACTCGTTTTCTATATTCCATCTCAGACAAGAAGAAAGAAACCTTTAA

Protein sequence

MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSNDLASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITELINQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL*

Homology

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BLAST of CSPI03G02050 vs. ExPASy Swiss-Prot
Match: Q5BLD8 (Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.0e-18
Identity = 69/169 (40.83%), Postives = 89/169 (52.66%), Query Frame = 0


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A VL  ++     +   D + K VIELGAGTGL G+ AALLGAN V +TD EP L 
Sbjct: 46  VWDAAVVLCMFL----EMGKVDLKGKRVIELGAGTGLVGIVAALLGAN-VTITDREPALE 105

Query: 95  GLLENVDAN---GVGDRVEVRELVWGSN-DLASQANELGEFDLILMSDLFYDSEEMPHLA 154
            L  NV  N   G    V+V EL WG N DL  Q    G +DLIL +D+ Y  E  P L 
Sbjct: 106 FLTANVHENIPQGRQKAVQVSELTWGENLDLYPQ----GGYDLILGADIVYLEETFPALL 165

Query: 155 QVLKIISGTDTKIWAASEIRPWTLECITELINQGFKITESPVQHDESHE 200
           Q L+ +S  DT +  +  IR    E     + Q F + E  V +D   +
Sbjct: 166 QTLEHLSSGDTVVLLSCRIRYERDERFLTELRQRFSVQE--VHYDSQRD 203

[Close]

BLAST of CSPI03G02050 vs. ExPASy Swiss-Prot
Match: A4FV98 (EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 9.1e-15
Identity = 59/166 (35.54%), Postives = 87/166 (52.41%), Query Frame = 0


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A  L  +  +Q    + DFR K VIELGAGTG+ G+ AAL G + V +TD+  +L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLVLE 115

Query: 95  GLLENVDAN-GVGDRVEVRELVWGSNDLASQANELGEFDLILMSDLFYDSEEMPHLAQVL 154
            +  NV AN   G R +VR L WG      Q    G++DL+L +D+ Y     P L   L
Sbjct: 116 QIQGNVQANVPPGGRAQVRALSWG----IDQHVFPGDYDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIISGTDTKIWAASEIRP--WTLECITELINQGFKITESPVQHDES 198
           + + G    I+ AS++R    T      L+ Q F++  +    DE+
Sbjct: 176 RHLCGPHGTIYLASKMREEHGTESFFQHLLPQHFQLELAQRDEDEN 212

[Close]

BLAST of CSPI03G02050 vs. ExPASy Swiss-Prot
Match: Q8C436 (Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=2 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.0e-14
Identity = 66/183 (36.07%), Postives = 96/183 (52.46%), Query Frame = 0


Query: 32  GSWVWNSAFVLAKWMATQCNLFD--FDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDV 91
           G  VW++A VL+K++ T     D      +++V+ELG+GTG  GL AA LGA+ V++TD+
Sbjct: 39  GCVVWDAAIVLSKYLETPGFSGDGAHALSRRSVLELGSGTGAVGLMAATLGAD-VIVTDL 98

Query: 92  EPLLPGLLENVDANG--VGDRVEVRELVWGSNDLASQANELGEFDLILMSDLFYDSEEMP 151
           E L   L  N+D N   V   V+ + L WG +       +L   D ILM+D  Y  E + 
Sbjct: 99  EELQDLLKMNIDMNKHLVTGSVQAKVLKWGED-----IEDLMSPDYILMADCIYYEESLE 158

Query: 152 HLAQVLKIISGTDTKIWAASEIR-----PWTLECITELINQGFKITESPV-QHDESH-EE 204
            L + LK +SG++T I    E R     P   +   EL+   F   E P+ +HDE +  E
Sbjct: 159 PLLKTLKDLSGSETCIICCYEQRTMGKNPEIEKKYFELLQLDFDFEEIPLDKHDEEYRSE 215

[Close]

BLAST of CSPI03G02050 vs. ExPASy Swiss-Prot
Match: Q8WXB1 (Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A PE=1 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 4.5e-14
Identity = 56/139 (40.29%), Postives = 77/139 (55.40%), Query Frame = 0


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A VL    +T   +   + R ++ +ELGAGTGL G+ AALLGA+ V +TD +  L 
Sbjct: 46  VWDAAIVL----STYLEMGAVELRGRSAVELGAGTGLVGIVAALLGAH-VTITDRKVALE 105

Query: 95  GLLENVDAN---GVGDRVEVRELVWGSNDLASQANELGEFDLILMSDLFYDSEEMPHLAQ 154
            L  NV AN    +  +  V+EL WG N L S +   GEFDLIL +D+ Y  E    L Q
Sbjct: 106 FLKSNVQANLPPHIQTKTVVKELTWGQN-LGSFSP--GEFDLILGADIIYLEETFTDLLQ 165

Query: 155 VLKIISGTDTKIWAASEIR 171
            L+ +    + I  A  IR
Sbjct: 166 TLEHLCSNHSVILLACRIR 176

[Close]

BLAST of CSPI03G02050 vs. ExPASy Swiss-Prot
Match: Q96AZ1 (EEF1A lysine methyltransferase 3 OS=Homo sapiens OX=9606 GN=EEF1AKMT3 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.3e-13
Identity = 57/166 (34.34%), Postives = 85/166 (51.20%), Query Frame = 0


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A  L  +  +Q    + DFR K VIELGAGTG+ G+ AAL G + V +TD+   L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLALE 115

Query: 95  GLLENVDAN-GVGDRVEVRELVWGSNDLASQANELGEFDLILMSDLFYDSEEMPHLAQVL 154
            +  NV AN   G + +VR L WG +     AN    +DL+L +D+ Y     P L   L
Sbjct: 116 QIQGNVQANVPAGGQAQVRALSWGIDHHVFPAN----YDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIISGTDTKIWAASEIRP--WTLECITELINQGFKITESPVQHDES 198
           + +      I+ AS++R    T      L+ Q F++  +    DE+
Sbjct: 176 QHLCRPHGTIYLASKMRKEHGTESFFQHLLPQHFQLELAQRDEDEN 212

[Close]

BLAST of CSPI03G02050 vs. ExPASy TrEMBL
Match: A0A0A0L6N4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.0e-121
Identity = 218/221 (98.64%), Postives = 220/221 (99.55%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND
Sbjct: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           L SQANELGEFDLILMSDLFY+SEEMPHLAQVLKIISGTDTKIWAASEIRPWT+ECITEL
Sbjct: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 222
           INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 221

[Close]

BLAST of CSPI03G02050 vs. ExPASy TrEMBL
Match: A0A5A7UCU5 (Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G001920 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.0e-114
Identity = 207/222 (93.24%), Postives = 216/222 (97.30%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFD RQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAG GLPGLTAALLGANRV+LTDV+PLL GLLENVDAN +GDRVEVRELVWGSN+
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTD+KIWAASEIRPWT+ECITEL
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQH-DESHEEDNHKILDSFSIFHLRQEERNL 222
           INQGFKITESPVQH DESHEEDNHK+LDS+SIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLRQEERNL 222

[Close]

BLAST of CSPI03G02050 vs. ExPASy TrEMBL
Match: A0A1S3BKF2 (protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo OX=3656 GN=LOC103490817 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.0e-114
Identity = 207/222 (93.24%), Postives = 216/222 (97.30%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFD RQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAG GLPGLTAALLGANRV+LTDV+PLL GLLENVDAN +GDRVEVRELVWGSN+
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTD+KIWAASEIRPWT+ECITEL
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQH-DESHEEDNHKILDSFSIFHLRQEERNL 222
           INQGFKITESPVQH DESHEEDNHK+LDS+SIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLRQEERNL 222

[Close]

BLAST of CSPI03G02050 vs. ExPASy TrEMBL
Match: A0A6J1E9L9 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 GN=LOC111431122 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.0e-93
Identity = 175/223 (78.48%), Postives = 195/223 (87.44%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISD N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFDFR K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRVEVRELVWG ++
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
             S+ANE GEFDLILMSDLFYDSE+M  LA +LKIISG ++KIWAASEIRPWT+EC+TE 
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 INQGFKITESPVQHDE-SHEEDNHKILDSFSIFHLR--QEERN 221
           +N+GFK+ ES VQ DE S E+D+H ILDSFSIFHL+   EERN
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERN 223

[Close]

BLAST of CSPI03G02050 vs. ExPASy TrEMBL
Match: A0A6J1HN15 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=LOC111465095 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.3e-92
Identity = 174/223 (78.03%), Postives = 193/223 (86.55%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISDRN+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFD R K
Sbjct: 31  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDK 90

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRV+VRELVWG ++
Sbjct: 91  TVIELGAGVGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLVDRVQVRELVWGLDE 150

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
             S+ANELGEFDLILMSDLFYDSE+M  LA VLKIISG ++KIWAASEIRPWT+EC+ E 
Sbjct: 151 SVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTVECLKEF 210

Query: 181 INQGFKITESPVQHDESHEE-DNHKILDSFSIFHLR--QEERN 221
           +N+GFK+ ES VQ DE  EE D+H ILD FSIFHL+   EERN
Sbjct: 211 MNEGFKVEESQVQLDEPREEDDDHAILDLFSIFHLQPPDEERN 253

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: XP_004150176.1 (protein N-lysine methyltransferase METTL21A [Cucumis sativus])

HSP 1 Score: 444.9 bits (1143), Expect = 4.1e-121
Identity = 218/221 (98.64%), Postives = 220/221 (99.55%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND
Sbjct: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           L SQANELGEFDLILMSDLFY+SEEMPHLAQVLKIISGTDTKIWAASEIRPWT+ECITEL
Sbjct: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 222
           INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 221

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: XP_008448745.1 (PREDICTED: protein N-lysine methyltransferase METTL21A-like [Cucumis melo] >KAA0053074.1 protein N-lysine methyltransferase METTL21A-like [Cucumis melo var. makuwa])

HSP 1 Score: 420.6 bits (1080), Expect = 8.3e-114
Identity = 207/222 (93.24%), Postives = 216/222 (97.30%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFD RQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAG GLPGLTAALLGANRV+LTDV+PLL GLLENVDAN +GDRVEVRELVWGSN+
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTD+KIWAASEIRPWT+ECITEL
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQH-DESHEEDNHKILDSFSIFHLRQEERNL 222
           INQGFKITESPVQH DESHEEDNHK+LDS+SIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLRQEERNL 222

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: XP_022923423.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata])

HSP 1 Score: 352.8 bits (904), Expect = 2.1e-93
Identity = 175/223 (78.48%), Postives = 195/223 (87.44%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISD N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFDFR K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRVEVRELVWG ++
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
             S+ANE GEFDLILMSDLFYDSE+M  LA +LKIISG ++KIWAASEIRPWT+EC+TE 
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 INQGFKITESPVQHDE-SHEEDNHKILDSFSIFHLR--QEERN 221
           +N+GFK+ ES VQ DE S E+D+H ILDSFSIFHL+   EERN
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERN 223

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: XP_023553140.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352.8 bits (904), Expect = 2.1e-93
Identity = 176/223 (78.92%), Postives = 193/223 (86.55%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISDRN+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFDFR K
Sbjct: 24  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 83

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRVEVRELVWG ++
Sbjct: 84  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 143

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
             S+ANE GEFDLILMSDLFYDSE+M  LA +LKIISG  +KIWAASEIRPWT+EC+TE 
Sbjct: 144 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKHSKIWAASEIRPWTVECLTEF 203

Query: 181 INQGFKITESPVQHDESHEE-DNHKILDSFSIFHLR--QEERN 221
            N+GFK+ ES VQ DE  EE D+H ILDSFSIFHL+   EERN
Sbjct: 204 TNEGFKVEESQVQLDEPREEDDDHTILDSFSIFHLQPPDEERN 246

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: KAG7015681.1 (EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 350.5 bits (898), Expect = 1.1e-92
Identity = 174/223 (78.03%), Postives = 194/223 (87.00%), Query Frame = 0


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M V+EFQISD N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMAT+C LFDFDFR K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATRCKLFDFDFRDK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
            VIELGAG GLPGL AALLGA RV+LTDVEPLLPGLL NVDANG+ DRVEVRELVWG ++
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
             S+ANE GEFDLILMSDLFYDSE+M  LA +LKIISG ++KIWAASEIRPWT+EC+TE 
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 INQGFKITESPVQHDESHEE-DNHKILDSFSIFHLR--QEERN 221
           +N+GFK+ ES VQ DE  EE D+H ILDSFSIFHL+   EERN
Sbjct: 181 MNEGFKVEESQVQLDEPREEVDDHAILDSFSIFHLQPPDEERN 223

[Close]

BLAST of CSPI03G02050 vs. TAIR 10
Match: AT5G44170.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.9e-15
Identity = 54/193 (27.98%), Postives = 92/193 (47.67%), Query Frame = 0


Query: 27  GRVLTGSWVWNSAFVLAKWMATQCNLFD-------------FDFRQKNVIELGAGTGLPG 86
           G +  G+ VW  + +L+K+      L               FDFR++  IELG G G+ G
Sbjct: 25  GSMHVGTSVWPCSLILSKFAERWSTLDSSSSTTSPNPYAELFDFRRRRGIELGTGCGVAG 84

Query: 87  LTAALLGANRVLLTDVEPLLPGLLENVDAN--GVGDRVEVRELVWGSNDLASQANELGEF 146
           +   LLG   ++LTD+ P++P L  N+  N   +G  ++   + W + D  S       F
Sbjct: 85  MAFYLLGLTEIVLTDIAPVMPALKHNLKRNKTALGKSLKTSIVYWNNRDQISALKP--PF 144

Query: 147 DLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIR-PWTLECITELINQGFKITES 201
           DL++ +D+ Y  E +  L   ++++   D  +    +IR P   +   EL +  FKI + 
Sbjct: 145 DLVIAADVVYIEESVGQLVTAMELLVADDGAVLLGYQIRSPEADKLFWELCDIVFKIEKV 204

[Close]

BLAST of CSPI03G02050 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.0e-13
Identity = 50/146 (34.25%), Postives = 74/146 (50.68%), Query Frame = 0


Query: 30  LTGSWVWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDV 89
           +TGS +W+S  VL K++    +        K ++ELG+G GL G  AALLG N V LTD+
Sbjct: 117 VTGSVMWDSGVVLGKFLEHSVDSKVLSLEGKKIVELGSGCGLVGCIAALLGGNAV-LTDL 176

Query: 90  EPLLPGLLENVDAN----GVGDRVEVRELVWGSNDLASQANELGEF-DLILMSDLFYDSE 149
              L  L +N+  N           V+ELVWG +      + +  F D +L SD+ Y  E
Sbjct: 177 PDRLRLLKKNIQTNLHRGNTRGSAIVQELVWGDD---PDPDLIEPFPDYVLGSDVIYSEE 236

Query: 150 EMPHLAQVLKIISGTDTKIWAASEIR 171
            + HL + L  +    T I+ + E+R
Sbjct: 237 AVHHLVKTLLQLCSDQTTIFLSGELR 258

[Close]

BLAST of CSPI03G02050 vs. TAIR 10
Match: AT1G08125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-12
Identity = 54/200 (27.00%), Postives = 91/200 (45.50%), Query Frame = 0


Query: 16  HELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFD---FRQKNVIELGAGTGLP 75
           HELD   D  +  +  G+ VW+++ V AK++        F     + K  IELGAG G+ 
Sbjct: 21  HELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVA 80

Query: 76  GLTAALLGANRVLLTDVEPLLPGLLENVDAN----------GVGDRVEVRELVWGSNDLA 135
           G   A+LG + V+ TD + +LP L  NV+ N               + V EL WG+ D  
Sbjct: 81  GFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHI 140

Query: 136 SQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTL-ECITELI 195
           +       FD ++ +D+ Y  + +  L + +  +SG  T +    EIR   + E + ++ 
Sbjct: 141 TAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMW 200

Query: 196 NQGFKITESPVQHDESHEED 202
              F++   P    +   +D
Sbjct: 201 KDNFEVKTIPRSKMDGEYQD 215

[Close]

BLAST of CSPI03G02050 vs. TAIR 10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-12
Identity = 54/200 (27.00%), Postives = 91/200 (45.50%), Query Frame = 0


Query: 16  HELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFD---FRQKNVIELGAGTGLP 75
           HELD   D  +  +  G+ VW+++ V AK++        F     + K  IELGAG G+ 
Sbjct: 28  HELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVA 87

Query: 76  GLTAALLGANRVLLTDVEPLLPGLLENVDAN----------GVGDRVEVRELVWGSNDLA 135
           G   A+LG + V+ TD + +LP L  NV+ N               + V EL WG+ D  
Sbjct: 88  GFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHI 147

Query: 136 SQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTL-ECITELI 195
           +       FD ++ +D+ Y  + +  L + +  +SG  T +    EIR   + E + ++ 
Sbjct: 148 TAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMW 207

Query: 196 NQGFKITESPVQHDESHEED 202
              F++   P    +   +D
Sbjct: 208 KDNFEVKTIPRSKMDGEYQD 222

[Close]

BLAST of CSPI03G02050 vs. TAIR 10
Match: AT3G50850.1 (Putative methyltransferase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 3.1e-10
Identity = 44/126 (34.92%), Postives = 67/126 (53.17%), Query Frame = 0


Query: 61  NVIELGAGTGLPGL-TAALLGANRVLLTDVEPLLPGLLENVDANG-----VGDRVEVREL 120
           N++ELG+GTG+ G+  AA LGAN V +TD+  ++  L  N DAN       G +V V  L
Sbjct: 91  NIVELGSGTGIVGIAAAATLGAN-VTVTDLPNVIENLKFNADANAQVVAKFGGKVHVASL 150

Query: 121 VWGSNDLASQANELGE-FDLILMSDLFYDSEEMPHLAQVLK--IISGTDTKIWAASEIRP 178
            WG  D       LG+  DLIL SD+ Y       L + L+  ++ G+  +++  + ++ 
Sbjct: 151 RWGEID---DVESLGQNVDLILASDVVYHVHLYEPLLKTLRFLLLEGSSERVFLMAHLKR 210

The following BLAST results are available for this feature:

BLAST of CSPI03G02050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5BLD8	3.0e-18	40.83	Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a P...	[more]
A4FV98	9.1e-15	35.54	EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1	[more]
Q8C436	2.0e-14	36.07	Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=...	[more]
Q8WXB1	4.5e-14	40.29	Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A ...	[more]
Q96AZ1	2.3e-13	34.34	EEF1A lysine methyltransferase 3 OS=Homo sapiens OX=9606 GN=EEF1AKMT3 PE=1 SV=1	[more]

BLAST of CSPI03G02050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6N4	2.0e-121	98.64	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1	[more]
A0A5A7UCU5	4.0e-114	93.24	Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BKF2	4.0e-114	93.24	protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1E9L9	1.0e-93	78.48	protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1HN15	3.3e-92	78.03	protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=...	[more]

BLAST of CSPI03G02050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150176.1	4.1e-121	98.64	protein N-lysine methyltransferase METTL21A [Cucumis sativus]	[more]
XP_008448745.1	8.3e-114	93.24	PREDICTED: protein N-lysine methyltransferase METTL21A-like [Cucumis melo] >KAA0...	[more]
XP_022923423.1	2.1e-93	78.48	protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata]	[more]
XP_023553140.1	2.1e-93	78.92	protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo]	[more]
KAG7015681.1	1.1e-92	78.03	EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of CSPI03G02050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44170.1	1.9e-15	27.98	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G73320.1	1.0e-13	34.25	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.1	1.1e-12	27.00	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.2	1.1e-12	27.00	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT3G50850.1	3.1e-10	34.92	Putative methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019410	Lysine methyltransferase	PFAM	PF10294	Methyltransf_16	coord: 31..170 e-value: 4.9E-29 score: 101.3
IPR019410	Lysine methyltransferase	PANTHER	PTHR14614	HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN	coord: 1..218
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..208 e-value: 3.5E-44 score: 152.8
None	No IPR available	PANTHER	PTHR14614:SF123	OSJNBA0070O11.4 PROTEIN	coord: 1..218
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 61..154 e-value: 3.8738E-7 score: 45.1135
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 48..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G02050.1	CSPI03G02050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity