CSPI03G01910 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G01910
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	beta-amyrin 11-oxidase-like
Location	Chr3: 1352248 .. 1358288 (-)
RNA-Seq Expression	CSPI03G01910
Synteny	CSPI03G01910

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTGATGCTAAATTGGTTGTTGTTGATTGTTCCTTTTGTGAGTTTTGTTTTAGGGTTTGGGGTTTTGAAGAGATTGAATAATTTGTATTATGCATTGAAATTAAGGAAGAAATGGGATGAACTTCCTCCTGGTGATCTATCTTGGCCACTTATTGGTTCTACTCTATCCTTTCTCAAATATTTCACTTTTGGTCCACCAGACGGTTTCATTCGTGACTTCTCAAGAAGGTATATATGTCCTCACTCTTTTGCATCCAGCCATCCATATTCTACACATAATATATTGTGAGACAAATAATGATTTTCAAATAAGTTCTCCACTCTTATTTATTTGAAAACCTTCTTTTAAATTAGTTATGTTATTCAAATTCATGAATTCATGTGTATATATATTATATTTTAGCATGACATCAGGTATGTATATAACAACTGGTTTCGAGATATGATGAGTGTGCTACATACGCAAGTGTTAATTTAGTTGAAATTTTTCGATGCACCCCTTGATCTTGTTCTACTTTGTTAAAAAAAATACTTAAGTTAGAAATTCAATTCTTCATCCTCAAAATTATATATATAAAAAAACCCTACTCAATTACTACAACAAGAAACTTTGGGGTGTTTAGATCAGAGTTAAGTTTACTATTATCGTGATTGAATTAATCTATAAATATCATCGTGGTTGAGTTGATAGAAACAATAATCAACTTCAAAACCTGCTTTTCTTTAAACATGTCAAGTTAACAATATTTTGTTTTCTAGTTTTCAAATTTGGAAATGTGTGTGTTTTCCTTGTATTTCTCACCATTATTCTATCAATTAAGAAACGTTAAATTCCAAACAACAATATTATTTCTTTATTTTTTATTTTTGACTTAATTTTTAAAACTATAAAAAAATGGATAATAAAACAACAAAATTTATTATTTATAGCTAAAACTAGGAGTGTAGAAATAGCCCGAATAATGTGAATAACTCTCCAACCCGAACTACTTAACCCATATTATAAAAGTTGAGTTGGATTAGTAATTTGGGTTGGGTTGGGTTAAAAATTTGGTTTTTTTTTTGGTGTGATTTAGGGTTGAAGAATTCATTTCAACCAGCCCAACCGAAATTAATTATATATATAAATTTTATTATTAATTAATTAGCATAAATTTAGGTCACACACGAGACTTTCCTACTTCACGAGTTAACCGTCTCACATCTCTCTATGGTGCCAATTCTTCCTCTTGTCTCAACCTCGCTCATCCCACACTGTCATCAGCGCGTTATCTTATTTTTCCTTTCGTTTTAACTTCTTCTTTGTGTTCATTGAAGATTTGAATTTTTGAATGTGTGTATTTATCAATATGACCCGTACAAAAATTTTAAATTTAACAAATATTAGAAATGAAAGAAAAAACATACCATCCACTGGGTTGGGTTGGAGATACTATATATTTAGGTTGCCAACCCAACTAACCCAACCCAACTTAGTTTTTTATTTAGATTTTTATTTTTTTTCTAAGTTACAACTCAAAGCCTTTGTCAATAATTTTTTTAGATTCGTCACTAGTTGGAAATGTCCCCCAACCCAACCCAACTTGTTTACACCCCTAATTAAAAGTTAAGTCGAAATTTGAGATGCCTCGGTGTCAACCTTTTTCTTATATCAATGCAATTTTGGACTGAGGTATGATGCAGGGGCTTTAGAAAGTATGTAAATTGTCAACCTAGTTGAAATGTTTAGATAATTCATTGTTCCTTCGACCATGGTAAGATTAAAGTTAAGCCAACATTTAAAAACTTTAATATAAGTTTGAATTTTGAAAATCAAATGATCATGTGAAAGAACATTGATTAGAAGAGATCGTGGAACTCACTATAAATACGTCAAAAGAAAAAAAATTGTAATAGTTAGTCAACTCTTTGGCAACTGTGTTATTTGGTTTTGGAGATTTGAAAATTTTGTTTGTGTTTTCTCCCGTATCTCTTATTGTCATCATAGTTTCATCTTTCTCAAGCATATAATTCTTTGTCATATTCTCATAGCTAAATCCACAACTAATAGTTTTCTTTAGTAACTTTTACTTCTCCAAAACATGATTTTGATTTTGAAAACATTTGCAAAGTAAATAGATCAAAAAGCAACTCACAACTGATATTAAATAATTAAACTTTCAACGTTTTAAAAAACATTTATATACATAGATATAAACGTATCAATGTCTATCACGAATAGATTCCAACTTTTTTTGAGAGTTGACAATAATTTTTTTATACGTTCTCTTCTTTTTCTCTTTTAAAATATAATTTTCAGCCCTCCAACACAAGTAATGGAAAAAATCTTTATCTTCTTACATCGACAAAAAGTATTATTTATAAAATTTGTTATTGTTTTTTTTTTATAAAAATGGTTATTCTTTTAGGTATGATAGTAACTTCCATATATATGATATATATGGTAGACATGTGAAAACATTATTAATATTAATGTAAATTCTACAAGTGAGGCTTAATCACATTTCTTCTTACATTATTTATGTTGCATAGGTGGCCTATTTATCCATTGGTTATTGTAATGTAATATACACAATATCTTAATCAACAAAATATTGATCCACAATTAAAATAAAAAATTGTTACGACATTACCCAAAATATTACCAAATGTTGAAATCATTACTAGAGTATTAATATATTAATGACTACCATAATCTTTTACTTTGTACCGTATTATAACTTCTTTTTTCTTTTTCTTTTTCTTTTTTTTGTGAAAATACGTACTGAATTCTGAAACAAAAAGTTTTACACTATAGAGTAAAACTATAGAATATTAATTTGATTTTAAAAGTATGGTATGTTTATCTTTAAAATTAAAACTACGAGATTTCACGTGCTTCCAAGTAGTTACTAATTATTTTATTTTTCCTTTTCTTCTTCTTTGATTAGATATGGGAAAATTGATATGTACAAGACTCACATATTTGGAAAACCAACTATAATCGTCTGCAAACCTGAAATATGTCGTCAAGTATTAACTGATGAAACCAAGTTCATTCCTAGTTATCCTACATCCATCACAGCCGTATTTGGTAAAAAATCACTGCTTCAAGTACCGAAAGAGGAGCATCGGAAGCTCCGACGGCTGACAATGGCACCGATCAGTGGCCATGCAGCGCTCGAGATGTACATTGATCATATTGAACACACTGTGATTAGTGGGTTGGAGGAGTGGTCAAGCATGAAGAAGCCATTGGAATTATTAACAACGATAAAGCAACTTACCTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACTCCAATAAAAAGCACTTCTATAAGAGAAATGGAGACTTTGAATGAGAATATTGCACTTGCATTCTTAACCCTGCCACTCAACTTTCCTGGCTTACCCTTCCATAAAGCACTCATGGTTAGAACAAAAATCATTTCTAATATAAATGTGACAGAGTTTCGATAACTTTTATTATATATGTGACATATATTTTAAATTAATCTAATCTACAATTCACTATATCATATGGTATAACAAAAAGAAATGTATAAATTATATGTAGGCTGGAAAAAGATTGAATGAAATACTTCAATCAATTGTGGATGAGAAGAGAATGGTGAAGAAAAGCAAAGGGGAAAATTGGGAAGCAAAAGATATGATGGATTTGTTGATGGAAGTGAGAGATGAAGATGGTGAAGGATTTGACGATGAGACCATTACAGAGATAATTTTTGGCATGCTATTTGGAGGCCAAGAGACTACAGCTTTCACTACAATGTGGGCAGTTTTGTTTCTTACAGATAATCCACAAGTATTCCAAAAGGCCAAGGTTAGACTCTTCTTTAATTTCCTAACTCATGTTTAAATTGCATAATAATGTGTATATGAGATATAAATGAAACTTAATTAAGATATATATGATCAAGATTTAGACTACAAATCATATCGACATTTTAAAGTATGTTATTTTGATTTTGAGATACATGATAAAGAGACGCTATATGGTGTTTTTTTCGAAAAAAAAGCATGTTTGTGTTGGTTGTTTGAAAAATATATCAATACTAACAAAACCATCAAAACACTACTTTTGTAAAAATATGAATGAAAATATATCGTTAAAATGTTCGTGTCAAGGGACATTACTTAAAAAATTAGAAAAGTAAAAAAATAAAAAATAAATTTAAATTAGTAAATAAACCTATTATTATTATGATATTTAAACCAGATTAGCATGTTTATTATTATTTATATTACACCATTTATTATATATATATTGATTCACTTCAAGTCGATGTCAAACTGTTTGAAACGTAAAAAATATTGATATATCAACGGAACATCTGAAAATTTAATACAATAGCCATATGATCACAAGTCGATGTCAAACTGTTTGAAACGTAAAAATATTGATATATCAACGGAACATATGAAAATTTAATACAATAGCCATATGATCACGACATGGATAACATAGTAGTATAGTGTTTTTTTTATTAATTTTATTATTATGAAATGAATTATTATTTTGATGGTTAAGTGTTAGATTGGTTTTAGGAAGAACAAGAAGATATCATTAGAAGAAGAGCCTCAACACAGAAGGGTGTAAGTCTATCAGAGATTAAACAAATGAAATTTCTTTCTCAGGTATTTTTATAATTTTAATTAATTTCCTTTGATCTTTTTTTAGGCACAAAACCATATATTACTATACCTTTCAGAATTGAATATAGTATGTTCTAAGTTCACAATGCTCATTATATTGTTGCTTCTTTTAATTTTGGACTTTAAGTTAGCTTGGTAATCAATTTTTATTGCATAATGTTTTTAAATTCTTAACATAAAAGAATTAATGTTAAAGTTTTTCGTGATTAATTGTTGAGTACTTTTTTTACGATTGATCCCTTCAAGATTAAAGATTTGTCCTCTTCACAAATTTATGAAATATAACTTTTTTTGATTAAGTAATAGCACACTACCACATTTTAAAGGATAACCATGTGTATCATAGTCCTAATTAGACGAGGACGACTTTCTATTGCAATTATATGCGATTTCTCCACCCTATATATTCACGTAACCATGCCCCTAATGCATAAAGATTGATTTTCATAATGTCTTACCCTGTATTTATGGAATATTCTAACAAGCTATGATAAAAAATAAATGGCTTATAGAGATAATAGTCATCCTAACCCAATAAGAAATAAGTTAGAATACTTTAAAAGTTTGTGAGAGGGCAGGTACGGTCATTAATCATAAGGGCTTTCGTGAAAACAACCATTCAAACAAAAATATGTATTTACGTTACTCTTGGGTTTGACAACTAGTTCTAACTAATATGATTATCATAGAATAATAAATAATTTTTCAATAAACACCGTGAACAAGAGTATCTAAATAGAATTGCTACTACATATAGTTCAACTGGAAAATGGTTTTGTAATATCTTATATAGCTTTTTGAGTTTTTGATCTCTAGTTTGTTTTGTGCATTTTCATCGATCAATGATATCACTTTTGGTCTTGGGACGTGATGAGGGTGCTATAACAAAATGTCAACCAAGTTGAGATGTTTTGATGTACCCTCTTATCCTCTCTTGCCTTGTATATATTTTCAAAATCTAGTAATATTGAATTATAACTATTAATTTTATATATTCTTTTATATAATATCCAGGTTATAGATGAGACGCTTCGACTAAGTAGTGTTGCATTTATTTCTTTTCGTGAGGCAACAGAAGATATTGAAATTAATGGTTAGTTGATCGCTATATTTTTCTCATTCATCCCAAATTAGGTCTCAAATCGTACCCTTCTATAAAGGGGTTTGTGTAATATCCTTCTAGCTAAAGAAGTTAAAATATTTTTCCTGAGTGATTGTAATAACTTTGTATTGTATATTTTGATTGTTTTAGGTAAAATAATACCAAAGGGATGGAGAGTCATACTTTGGCTTCGAGGGCTTAACATGGACCAAAAACTGCATCCTTCCCCTCAACACTTCAATCCTTCAAGATGGGATGTATGCATTTATTAA

mRNA sequence

ATGGAGTTGATGCTAAATTGGTTGTTGTTGATTGTTCCTTTTGTGAGTTTTGTTTTAGGGTTTGGGGTTTTGAAGAGATTGAATAATTTGTATTATGCATTGAAATTAAGGAAGAAATGGGATGAACTTCCTCCTGGTGATCTATCTTGGCCACTTATTGGTTCTACTCTATCCTTTCTCAAATATTTCACTTTTGGTCCACCAGACGGTTTCATTCGTGACTTCTCAAGAAGATATGGGAAAATTGATATGTACAAGACTCACATATTTGGAAAACCAACTATAATCGTCTGCAAACCTGAAATATGTCGTCAAGTATTAACTGATGAAACCAAGTTCATTCCTAGTTATCCTACATCCATCACAGCCGTATTTGGTAAAAAATCACTGCTTCAAGTACCGAAAGAGGAGCATCGGAAGCTCCGACGGCTGACAATGGCACCGATCAGTGGCCATGCAGCGCTCGAGATGTACATTGATCATATTGAACACACTGTGATTAGTGGGTTGGAGGAGTGGTCAAGCATGAAGAAGCCATTGGAATTATTAACAACGATAAAGCAACTTACCTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACTCCAATAAAAAGCACTTCTATAAGAGAAATGGAGACTTTGAATGAGAATATTGCACTTGCATTCTTAACCCTGCCACTCAACTTTCCTGGCTTACCCTTCCATAAAGCACTCATGGCTGGAAAAAGATTGAATGAAATACTTCAATCAATTGTGGATGAGAAGAGAATGGTGAAGAAAAGCAAAGGGGAAAATTGGGAAGCAAAAGATATGATGGATTTGTTGATGGAAGTGAGAGATGAAGATGGTGAAGGATTTGACGATGAGACCATTACAGAGATAATTTTTGGCATGCTATTTGGAGGCCAAGAGACTACAGCTTTCACTACAATGTGGGCAGTTTTGTTTCTTACAGATAATCCACAAGTATTCCAAAAGGCCAAGGAAGAACAAGAAGATATCATTAGAAGAAGAGCCTCAACACAGAAGGGTGTAAGTCTATCAGAGATTAAACAAATGAAATTTCTTTCTCAGGTTATAGATGAGACGCTTCGACTAAGTAGTGTTGCATTTATTTCTTTTCGTGAGGCAACAGAAGATATTGAAATTAATGGTAAAATAATACCAAAGGGATGGAGAGTCATACTTTGGCTTCGAGGGCTTAACATGGACCAAAAACTGCATCCTTCCCCTCAACACTTCAATCCTTCAAGATGGGATGTATGCATTTATTAA

Coding sequence (CDS)

ATGGAGTTGATGCTAAATTGGTTGTTGTTGATTGTTCCTTTTGTGAGTTTTGTTTTAGGGTTTGGGGTTTTGAAGAGATTGAATAATTTGTATTATGCATTGAAATTAAGGAAGAAATGGGATGAACTTCCTCCTGGTGATCTATCTTGGCCACTTATTGGTTCTACTCTATCCTTTCTCAAATATTTCACTTTTGGTCCACCAGACGGTTTCATTCGTGACTTCTCAAGAAGATATGGGAAAATTGATATGTACAAGACTCACATATTTGGAAAACCAACTATAATCGTCTGCAAACCTGAAATATGTCGTCAAGTATTAACTGATGAAACCAAGTTCATTCCTAGTTATCCTACATCCATCACAGCCGTATTTGGTAAAAAATCACTGCTTCAAGTACCGAAAGAGGAGCATCGGAAGCTCCGACGGCTGACAATGGCACCGATCAGTGGCCATGCAGCGCTCGAGATGTACATTGATCATATTGAACACACTGTGATTAGTGGGTTGGAGGAGTGGTCAAGCATGAAGAAGCCATTGGAATTATTAACAACGATAAAGCAACTTACCTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACTCCAATAAAAAGCACTTCTATAAGAGAAATGGAGACTTTGAATGAGAATATTGCACTTGCATTCTTAACCCTGCCACTCAACTTTCCTGGCTTACCCTTCCATAAAGCACTCATGGCTGGAAAAAGATTGAATGAAATACTTCAATCAATTGTGGATGAGAAGAGAATGGTGAAGAAAAGCAAAGGGGAAAATTGGGAAGCAAAAGATATGATGGATTTGTTGATGGAAGTGAGAGATGAAGATGGTGAAGGATTTGACGATGAGACCATTACAGAGATAATTTTTGGCATGCTATTTGGAGGCCAAGAGACTACAGCTTTCACTACAATGTGGGCAGTTTTGTTTCTTACAGATAATCCACAAGTATTCCAAAAGGCCAAGGAAGAACAAGAAGATATCATTAGAAGAAGAGCCTCAACACAGAAGGGTGTAAGTCTATCAGAGATTAAACAAATGAAATTTCTTTCTCAGGTTATAGATGAGACGCTTCGACTAAGTAGTGTTGCATTTATTTCTTTTCGTGAGGCAACAGAAGATATTGAAATTAATGGTAAAATAATACCAAAGGGATGGAGAGTCATACTTTGGCTTCGAGGGCTTAACATGGACCAAAAACTGCATCCTTCCCCTCAACACTTCAATCCTTCAAGATGGGATGTATGCATTTATTAA

Protein sequence

MELMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLKYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVIDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRWDVCIY*

Homology

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BLAST of CSPI03G01910 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 6.1e-116
Identity = 199/408 (48.77%), Postives = 290/408 (71.08%), Query Frame = 0


Query: 15  VSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLKYFTFGPPDGFIRD 74
           V ++ G   ++ LN  YY +KLR+K   LPPGD+ WPLIG  LSF+K F+ G PD FI +
Sbjct: 15  VCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFINN 74

Query: 75  FSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSITAVFGKKSLLQVP 134
              +YG+  +YKTH+FG P+IIVC+P++CR+VLTD+  F   YP SI  +   + ++ V 
Sbjct: 75  LVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVS 134

Query: 135 KEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVI 194
             EHR  RRL  +PI GH AL MY++ +E  VI+ LEE SSMK P+ELL  +K+++FK I
Sbjct: 135 NAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAI 194

Query: 195 WNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSIVD 254
            ++FMGS+      I+++ +   ++     ++P+N PG  FHKAL A K+L +I+Q +VD
Sbjct: 195 VHVFMGSS--NQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVVD 254

Query: 255 EKR-MVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMW 314
           E+R M++    E  + KD++D+L+EV+DE+G   +DE I++++ G+LF G E+TA + MW
Sbjct: 255 ERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLMW 314

Query: 315 AVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVIDETLRLSSVAFI 374
           ++ +LT +P + +KAKEEQE+I R R S+QK +SL EIKQM +LSQVIDETLR +++AF 
Sbjct: 315 SITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDETLRCANIAFA 374

Query: 375 SFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRWD 422
           +FREAT D+ ING IIPKGWRV++W R ++MD + +P+P+ FNPSRWD
Sbjct: 375 TFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWD 420

[Close]

BLAST of CSPI03G01910 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 2.2e-94
Identity = 182/422 (43.13%), Postives = 269/422 (63.74%), Query Frame = 0


Query: 6   NWLLLIVPFVSFVLG-FG---VLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLK 65
           +W+ +  P +  VLG FG   +++++N   Y   L +    LPPGDL WP IG+ LSFL+
Sbjct: 6   SWIPVWFPLM--VLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLR 65

Query: 66  YFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSI 125
            F    PD F R   +RYG   +YK H+FG P+IIV   + CR+VLTD+  F P +PTS 
Sbjct: 66  AFKTSDPDSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTST 125

Query: 126 TAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLE 185
             + G+KS + +  EEH++LRRLT AP++GH AL  YI +IE  VI+ L++W+ M +  E
Sbjct: 126 MELIGRKSFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FE 185

Query: 186 LLTTIKQLTFKVIWNIFMGSTP--IKSTSIREMETLNENIALAFLTLPLNFPGLPFHKAL 245
            LT +++LTF++I  IF+ S    +     RE   LN  +      + +N PG  +H+AL
Sbjct: 186 FLTHLRKLTFRIIMYIFLSSESENVMDALEREYTALNYGVR----AMAVNIPGFAYHRAL 245

Query: 246 MAGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGM 305
            A K L    QSIV E+R  +K    +   KDM+D L+ V+DEDG+  DDE I +++   
Sbjct: 246 KARKTLVAAFQSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMY 305

Query: 306 LFGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQ 365
           L  G E++  T MWA +FL ++P+V Q+AK EQE I++ R   QKG+SL E ++M+FLSQ
Sbjct: 306 LNAGHESSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQ 365

Query: 366 VIDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSR 422
           V+DETLR+ + +  +FREA  D+E+NG +IPKGW+V+ W R +++D ++ P P+ F+P+R
Sbjct: 366 VVDETLRVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPAR 419

[Close]

BLAST of CSPI03G01910 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 342.4 bits (877), Expect = 7.2e-93
Identity = 182/419 (43.44%), Postives = 270/419 (64.44%), Query Frame = 0


Query: 3   LMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLKY 62
           L+L W  LI+    FVL + VLKR+N   Y  KL +K   LPPGDL WP+IG+  SFL+ 
Sbjct: 6   LILMWFPLII-LGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRA 65

Query: 63  FTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSIT 122
           F    P+ FI+ +  RYG+  +YK H+FG P ++V  PE CR+VLTD+  F   +P S  
Sbjct: 66  FKTSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTM 125

Query: 123 AVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLEL 182
            + G+KS + +  EEH++LRRLT AP++G  AL +YI  IE TV + LE+WS M + +E 
Sbjct: 126 KLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEF 185

Query: 183 LTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMAG 242
           L+ +++LTFKVI  IF+ S         E E  N N  +    + +N PG  +H+AL A 
Sbjct: 186 LSHLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVR--AMGINLPGFAYHRALKAR 245

Query: 243 KRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFG 302
           K+L    QSIV  +R  +K    +   KDM+D L++V+DE+G   DDE I +++   L  
Sbjct: 246 KKLVAAFQSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNA 305

Query: 303 GQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 362
           G E++   TMWA + + ++P + QKAKEEQE I+++RA  QK ++L E ++M +LSQVID
Sbjct: 306 GHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVID 365

Query: 363 ETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRWD 422
           ETLR+ + +  +FREA  D++++G IIPKGW+V+ W R +++D +++P P+ F+PSRW+
Sbjct: 366 ETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 417

[Close]

BLAST of CSPI03G01910 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.2e-90
Identity = 162/417 (38.85%), Postives = 257/417 (61.63%), Query Frame = 0


Query: 7   WLLLIVPFVSFVLGFGVLKRLNNLYYALKL-RKKWDELPPGDLSWPLIGSTLSFLKYFTF 66
           W+L        VL   V++R ++      L  ++   LPPG++ WP++GS  +FL+ F  
Sbjct: 18  WVLAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKS 77

Query: 67  GPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSITAVF 126
           G PD FI  F RR+G+  +Y+T +F  PTI+   PE C+QVL D+  F+  +P +   + 
Sbjct: 78  GNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLI 137

Query: 127 GKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKK-PLELLT 186
           G KS + +  ++HR++R+LT API+G  AL  Y+  I+ TV++ L  WSS +   +E LT
Sbjct: 138 GPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLT 197

Query: 187 TIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMAGKR 246
            ++++TFK+I  IFM        ++  +E    ++      + +N PG  +++AL A ++
Sbjct: 198 ELRRMTFKIIVQIFMSGA--DDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRK 257

Query: 247 LNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQ 306
           L  +LQ ++D +R       +   A DMMD L+E  DE G    D+ I +++   L  G 
Sbjct: 258 LVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGH 317

Query: 307 ETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVIDET 366
           E++   TMWA +FL +NP +F +AK EQE+I+R   +TQ G++L + K+M FLSQV+DET
Sbjct: 318 ESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDET 377

Query: 367 LRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRWD 422
           LR  +++F+SFR+AT DI +NG +IPKGW+V LW R ++MD +++P P+ FNPSRW+
Sbjct: 378 LRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWE 432

[Close]

BLAST of CSPI03G01910 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.5e-90
Identity = 158/379 (41.69%), Postives = 247/379 (65.17%), Query Frame = 0


Query: 43  LPPGDLSWPLIGSTLSFLKYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEI 102
           LPPG++ WPL+G   +FL+ F  G PD FI  F RR+G+  +Y++ +F  PT++V   E 
Sbjct: 72  LPPGEMGWPLVGGMWAFLRAFKSGKPDAFIASFVRRFGRTGVYRSFMFSSPTVLVTTAEG 131

Query: 103 CRQVLTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHI 162
           C+QVL D+  F+  +P +  A+ G +S + +P +EHR++R+LT API+G  AL  Y+  I
Sbjct: 132 CKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRIRKLTAAPINGFDALTGYLPFI 191

Query: 163 EHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALA 222
           + TV S L  W+     +E LT ++++TFK+I  IF+G     +T  R +E     +   
Sbjct: 192 DRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGGADQATT--RALERSYTELNYG 251

Query: 223 FLTLPLNFPGLPFHKALMAGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDE 282
              + +N PG  +  AL A +RL  +LQ ++DE+R   ++KG +    DMMD L+E +DE
Sbjct: 252 MRAMAINLPGFAYRGALRARRRLVAVLQGVLDERR-AARAKGVSGGGVDMMDRLIEAQDE 311

Query: 283 DGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRAST 342
            G   DD+ I +++   L  G E++   TMWA +FL +NP +F +AK EQE I+R   S+
Sbjct: 312 RGRHLDDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPDMFARAKAEQEAIMRSIPSS 371

Query: 343 QKGVSLSEIKQMKFLSQVIDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGL 402
           Q+G++L + ++M++LSQVIDETLRL +++F+SFR+AT D+ +NG +IPKGW+V LW R +
Sbjct: 372 QRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSV 431

Query: 403 NMDQKLHPSPQHFNPSRWD 422
           +MD +++P P  F+PSRW+
Sbjct: 432 HMDPQVYPDPTKFDPSRWE 447

[Close]

BLAST of CSPI03G01910 vs. ExPASy TrEMBL
Match: A0A1S3BKZ4 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490804 PE=3 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 3.5e-215
Identity = 369/422 (87.44%), Postives = 394/422 (93.36%), Query Frame = 0


Query: 1   MELMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFL 60
           MEL++NWLLLIVPFV FVLGFGVLKRLNNLYYALKL KKWDELPPGDLSWPL+GSTLSFL
Sbjct: 1   MELVINWLLLIVPFVGFVLGFGVLKRLNNLYYALKLGKKWDELPPGDLSWPLVGSTLSFL 60

Query: 61  KYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQV-LTDETKFIPSYPT 120
           KYFTFGPP+ FI DFSRRYGK+DMYKTHIFGKPTIIVCKPEICRQV LT+E KFIP YP 
Sbjct: 61  KYFTFGPPESFIADFSRRYGKLDMYKTHIFGKPTIIVCKPEICRQVLLTEEAKFIPGYPI 120

Query: 121 SITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKP 180
           ++T +FG+KSL QVPKEEHRKLRRLT APISGHAALEMYIDHIEHTVISGLEEWS  +KP
Sbjct: 121 TMTTIFGRKSLHQVPKEEHRKLRRLTTAPISGHAALEMYIDHIEHTVISGLEEWSRTEKP 180

Query: 181 LELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKAL 240
           LELLTTIK LT KVIWNIFMGSTPIKSTSIREME LNE+IALAF TLP+NFPG PFHKAL
Sbjct: 181 LELLTTIKHLTLKVIWNIFMGSTPIKSTSIREMENLNEDIALAFFTLPINFPGFPFHKAL 240

Query: 241 MAGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGM 300
           M  KRLNEILQSI+DEKRMVKKSKGENW+AKDMMDLLMEVRDEDGEGFDDETITE+IF M
Sbjct: 241 MGRKRLNEILQSILDEKRMVKKSKGENWKAKDMMDLLMEVRDEDGEGFDDETITEMIFSM 300

Query: 301 LFGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQ 360
           LFGGQET+AFTTMWAVLFLTDNP +FQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+LSQ
Sbjct: 301 LFGGQETSAFTTMWAVLFLTDNPHIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKYLSQ 360

Query: 361 VIDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSR 420
           VIDETLRL S+AF +FREAT D+EINGKIIPKGW+VILWLR L MD+KLHPSPQ FNPSR
Sbjct: 361 VIDETLRLGSIAFATFREATTDVEINGKIIPKGWKVILWLRELYMDEKLHPSPQQFNPSR 420

Query: 421 WD 422
           WD
Sbjct: 421 WD 422

[Close]

BLAST of CSPI03G01910 vs. ExPASy TrEMBL
Match: A0A1S3BKD3 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103490805 PE=3 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.4e-208
Identity = 354/421 (84.09%), Postives = 389/421 (92.40%), Query Frame = 0


Query: 1   MELMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFL 60
           MEL++NWLLLIVPFV FVLGFGVLKRLNNLYYALKL KKWDELPPGDLSWP IGSTLSFL
Sbjct: 1   MELVINWLLLIVPFVGFVLGFGVLKRLNNLYYALKLGKKWDELPPGDLSWPFIGSTLSFL 60

Query: 61  KYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTS 120
           KYFTFGPP+ FI DFSRRYGK+ MYKTHIFG  TIIVCKPEICRQVLTDE+KFIP+Y T+
Sbjct: 61  KYFTFGPPESFIADFSRRYGKVGMYKTHIFGNLTIIVCKPEICRQVLTDESKFIPAYHTT 120

Query: 121 ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPL 180
           +  +FGK+SL QV KEEH+KLRRL   PISGHA+LEMYIDHIE T ISG EEWSSM+KPL
Sbjct: 121 MKTLFGKRSLHQVSKEEHQKLRRLITIPISGHASLEMYIDHIEQTAISGFEEWSSMEKPL 180

Query: 181 ELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALM 240
           ELLT+IKQLTFKVIWNIFMGSTP KST+IREME+LN++I LAF T+P+NFPG  FHKAL 
Sbjct: 181 ELLTSIKQLTFKVIWNIFMGSTPTKSTTIREMESLNDDIVLAFFTMPINFPGFSFHKALK 240

Query: 241 AGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGML 300
           A KRL+EILQSI+DEK+MVKK KGE+WEAKDMMDLLMEVRDEDGEGFDDETITE+IFGML
Sbjct: 241 ARKRLHEILQSILDEKKMVKKRKGESWEAKDMMDLLMEVRDEDGEGFDDETITEMIFGML 300

Query: 301 FGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQV 360
           FGGQET+AFTTMWA+LFLTDNP +FQKAKEEQEDIIRRR STQKG+SLSEIKQMKFLSQV
Sbjct: 301 FGGQETSAFTTMWAILFLTDNPHIFQKAKEEQEDIIRRRVSTQKGISLSEIKQMKFLSQV 360

Query: 361 IDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRW 420
           IDETLRLSS+AF++FREAT D+EINGK IPKGW+VILWLR LNMD+KLHPSPQHFNPSRW
Sbjct: 361 IDETLRLSSIAFVTFREATVDVEINGKTIPKGWKVILWLRELNMDEKLHPSPQHFNPSRW 420

Query: 421 D 422
           D
Sbjct: 421 D 421

[Close]

BLAST of CSPI03G01910 vs. ExPASy TrEMBL
Match: A0A5A7UBF0 (Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001820 PE=3 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 8.0e-204
Identity = 353/416 (84.86%), Postives = 377/416 (90.62%), Query Frame = 0


Query: 20  GFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLKYFTFGPPDGFIRDFSRRY 79
           GFGVLKRLNNLYYALKL KKWDELPPGDLSWPL+GSTLSFLKYFTFGPP+ FI DFSRRY
Sbjct: 3   GFGVLKRLNNLYYALKLGKKWDELPPGDLSWPLVGSTLSFLKYFTFGPPESFIADFSRRY 62

Query: 80  GKIDMYKTHIFGKPTIIVCKPEICRQV-LTDETKFIPSYPTSITAVFGKKSLLQVPKEEH 139
           GK+DMYKTHIFGKPTIIVCKPEICRQV LT+E KFIP YP ++T +FG+KSL QVPKEEH
Sbjct: 63  GKLDMYKTHIFGKPTIIVCKPEICRQVLLTEEAKFIPGYPITMTTIFGRKSLHQVPKEEH 122

Query: 140 RKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIF 199
           RKLRRLT APISGHAALEMYIDHIEHTVISGLEEWS  +KPLELLTTIK LT KVIWNIF
Sbjct: 123 RKLRRLTTAPISGHAALEMYIDHIEHTVISGLEEWSRTEKPLELLTTIKHLTLKVIWNIF 182

Query: 200 MGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALM-------------AGKRL 259
           MGSTPIKSTSIREME LNE+IALAF TLP+NFPG PFHKALM             + KRL
Sbjct: 183 MGSTPIKSTSIREMENLNEDIALAFFTLPINFPGFPFHKALMERTKIISNTNATKSRKRL 242

Query: 260 NEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQE 319
           NEILQSI+DEKRMVKKSKGENW+AKDMMDLLMEVRDEDGEGFDDETITE+IF MLFGGQE
Sbjct: 243 NEILQSILDEKRMVKKSKGENWKAKDMMDLLMEVRDEDGEGFDDETITEMIFSMLFGGQE 302

Query: 320 TTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVIDETL 379
           T+AFTTMWAVLFLTDNP +FQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+LSQVIDETL
Sbjct: 303 TSAFTTMWAVLFLTDNPHIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKYLSQVIDETL 362

Query: 380 RLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRWD 422
           RL S+AF +FREAT D+EINGKIIPKGW+VILWLR L MD+KLHPSPQ FNPSRWD
Sbjct: 363 RLGSIAFATFREATTDVEINGKIIPKGWKVILWLRELYMDEKLHPSPQQFNPSRWD 418

[Close]

BLAST of CSPI03G01910 vs. ExPASy TrEMBL
Match: A0A6J1L1P3 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 3.2e-176
Identity = 303/417 (72.66%), Postives = 359/417 (86.09%), Query Frame = 0


Query: 5   LNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLKYFT 64
           +N + LI+P + FVL F VLKRLN LYYALKL KKW ELPPGDLSWPL+GSTLSFLK FT
Sbjct: 4   INGVFLILP-LGFVLVFEVLKRLNGLYYALKLGKKWGELPPGDLSWPLLGSTLSFLKSFT 63

Query: 65  FGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSITAV 124
            GPP+ FIR F+ RYGK+DMYKTHIFG+ +IIVCKPEICRQVL DETKF+P YP S+  +
Sbjct: 64  VGPPESFIRAFTTRYGKVDMYKTHIFGRASIIVCKPEICRQVLIDETKFVPGYPASMKTL 123

Query: 125 FGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLT 184
           FG+KSL +V K EHRKLRR+T APISGHAALEMYI+HIEHTVISGLEEWSS++KPL+LLT
Sbjct: 124 FGRKSLHRVSKVEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKLLT 183

Query: 185 TIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMAGKR 244
            IK+LTFK+IWNIFMGST I  T+ +EME L  +I+L F +LP+NFPG  FHK+L A KR
Sbjct: 184 EIKELTFKIIWNIFMGSTSIDYTT-KEMEALYNDISLGFFSLPINFPGFYFHKSLKARKR 243

Query: 245 LNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQ 304
           L+EIL SIV+EKR++KKSKGE+WEA+DMMDL++EV DEDGEG D+ETI ++IFG LF GQ
Sbjct: 244 LHEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQ 303

Query: 305 ETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVIDET 364
           ET+AFTTMWA++FLTD+P +FQKAKEEQEDI+ RR STQK ++ +E KQMKFLSQVIDET
Sbjct: 304 ETSAFTTMWAIIFLTDHPHLFQKAKEEQEDILSRRPSTQKSINFAEFKQMKFLSQVIDET 363

Query: 365 LRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRWD 422
           LRLS + F +FREAT D+ INGK+IPKGW+VILWLR L MD++LHPSPQ FNPSRWD
Sbjct: 364 LRLSGITFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWD 418

[Close]

BLAST of CSPI03G01910 vs. ExPASy TrEMBL
Match: A0A6J1E6E2 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 4.1e-176
Identity = 302/417 (72.42%), Postives = 360/417 (86.33%), Query Frame = 0


Query: 5   LNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLKYFT 64
           +N + LI+P + FVL F VLKRLN LYYALKL KKW ELPPGDLSWPL+GSTLSFLK FT
Sbjct: 4   INGVFLILP-LGFVLVFEVLKRLNGLYYALKLGKKWVELPPGDLSWPLLGSTLSFLKSFT 63

Query: 65  FGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSITAV 124
            GPP+ FIR F+ RYGK+DMYKTHIFG+ +I+VCKPEICRQVL +ETKF+P YP S+  +
Sbjct: 64  VGPPESFIRTFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLINETKFVPGYPASMKTL 123

Query: 125 FGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLT 184
           FG+KSL +V K EHRKLRR+T APISGHAALEMYI+HIEHTVISGLEEWSS++KPL+LLT
Sbjct: 124 FGRKSLHRVSKAEHRKLRRVTTAPISGHAALEMYIEHIEHTVISGLEEWSSVEKPLKLLT 183

Query: 185 TIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMAGKR 244
            IK+LTFK+IWNIFMGST I  T+ +EME L  +I+L F +LP+NFPG  FHK+L A KR
Sbjct: 184 EIKELTFKIIWNIFMGSTSIDYTT-KEMEALYNDISLGFFSLPINFPGFYFHKSLKARKR 243

Query: 245 LNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQ 304
           L+EIL SIV+EKR++ KSKGE+WEAKDMMDL++EV DEDGEG D+ETI ++IFG LF GQ
Sbjct: 244 LHEILLSIVNEKRLMMKSKGESWEAKDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQ 303

Query: 305 ETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVIDET 364
           ET+AFTTMWA++FLTD+P +FQKAKEEQEDI+ RR ST KG++L+E KQMKFLSQVIDET
Sbjct: 304 ETSAFTTMWAIIFLTDHPHIFQKAKEEQEDILSRRPSTHKGINLAEFKQMKFLSQVIDET 363

Query: 365 LRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRWD 422
           LRLS + F +FREAT D++INGK+IPKGW+VILWLR L MD++LHPSPQ FNPSRWD
Sbjct: 364 LRLSGITFATFREATTDVKINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWD 418

[Close]

BLAST of CSPI03G01910 vs. NCBI nr
Match: XP_031739358.1 (beta-amyrin 11-oxidase isoform X1 [Cucumis sativus])

HSP 1 Score: 798.1 bits (2060), Expect = 3.7e-227
Identity = 394/421 (93.59%), Postives = 404/421 (95.96%), Query Frame = 0


Query: 1   MELMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFL 60
           MELM+NWL LIVPF+ FVLGFGVLKRLNNLYYALKL KKWDELPPGDLSWPLIGSTLSFL
Sbjct: 1   MELMINWLSLIVPFLGFVLGFGVLKRLNNLYYALKLGKKWDELPPGDLSWPLIGSTLSFL 60

Query: 61  KYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTS 120
           KYFTFGPP+ FI DFSRRYGK+DMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTS
Sbjct: 61  KYFTFGPPERFIGDFSRRYGKLDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTS 120

Query: 121 ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPL 180
           ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEH+VISGLEEWSSMKKPL
Sbjct: 121 ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHSVISGLEEWSSMKKPL 180

Query: 181 ELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALM 240
           ELLTTIKQLTFKVIWNIFMGSTPIKSTSI EMETLNENIALAFLTLPLNFPGLPFHKALM
Sbjct: 181 ELLTTIKQLTFKVIWNIFMGSTPIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALM 240

Query: 241 AGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGML 300
           AGKRLNEI QS +DEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETI E+IFGML
Sbjct: 241 AGKRLNEIFQSYLDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETIRELIFGML 300

Query: 301 FGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQV 360
           FGGQETTAFTTMWAVLFLTDNP +FQKAKEEQEDIIRRRASTQKGVS SEIKQMKFLSQV
Sbjct: 301 FGGQETTAFTTMWAVLFLTDNPHIFQKAKEEQEDIIRRRASTQKGVSFSEIKQMKFLSQV 360

Query: 361 IDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRW 420
           IDETLRLSSVAF  FREAT D+EINGKIIPKGW+VI W RGLNMDQKLHPSPQ FNPSRW
Sbjct: 361 IDETLRLSSVAFSVFREATVDVEINGKIIPKGWKVIPWFRGLNMDQKLHPSPQQFNPSRW 420

Query: 421 D 422
           D
Sbjct: 421 D 421

[Close]

BLAST of CSPI03G01910 vs. NCBI nr
Match: XP_031739359.1 (beta-amyrin 11-oxidase isoform X2 [Cucumis sativus] >KAE8650036.1 hypothetical protein Csa_009692 [Cucumis sativus])

HSP 1 Score: 798.1 bits (2060), Expect = 3.7e-227
Identity = 394/421 (93.59%), Postives = 404/421 (95.96%), Query Frame = 0


Query: 1   MELMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFL 60
           MELM+NWL LIVPF+ FVLGFGVLKRLNNLYYALKL KKWDELPPGDLSWPLIGSTLSFL
Sbjct: 1   MELMINWLSLIVPFLGFVLGFGVLKRLNNLYYALKLGKKWDELPPGDLSWPLIGSTLSFL 60

Query: 61  KYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTS 120
           KYFTFGPP+ FI DFSRRYGK+DMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTS
Sbjct: 61  KYFTFGPPERFIGDFSRRYGKLDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTS 120

Query: 121 ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPL 180
           ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEH+VISGLEEWSSMKKPL
Sbjct: 121 ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHSVISGLEEWSSMKKPL 180

Query: 181 ELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALM 240
           ELLTTIKQLTFKVIWNIFMGSTPIKSTSI EMETLNENIALAFLTLPLNFPGLPFHKALM
Sbjct: 181 ELLTTIKQLTFKVIWNIFMGSTPIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALM 240

Query: 241 AGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGML 300
           AGKRLNEI QS +DEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETI E+IFGML
Sbjct: 241 AGKRLNEIFQSYLDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETIRELIFGML 300

Query: 301 FGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQV 360
           FGGQETTAFTTMWAVLFLTDNP +FQKAKEEQEDIIRRRASTQKGVS SEIKQMKFLSQV
Sbjct: 301 FGGQETTAFTTMWAVLFLTDNPHIFQKAKEEQEDIIRRRASTQKGVSFSEIKQMKFLSQV 360

Query: 361 IDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRW 420
           IDETLRLSSVAF  FREAT D+EINGKIIPKGW+VI W RGLNMDQKLHPSPQ FNPSRW
Sbjct: 361 IDETLRLSSVAFSVFREATVDVEINGKIIPKGWKVIPWFRGLNMDQKLHPSPQQFNPSRW 420

Query: 421 D 422
           D
Sbjct: 421 D 421

[Close]

BLAST of CSPI03G01910 vs. NCBI nr
Match: XP_008448717.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 757.3 bits (1954), Expect = 7.2e-215
Identity = 369/422 (87.44%), Postives = 394/422 (93.36%), Query Frame = 0


Query: 1   MELMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFL 60
           MEL++NWLLLIVPFV FVLGFGVLKRLNNLYYALKL KKWDELPPGDLSWPL+GSTLSFL
Sbjct: 1   MELVINWLLLIVPFVGFVLGFGVLKRLNNLYYALKLGKKWDELPPGDLSWPLVGSTLSFL 60

Query: 61  KYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQV-LTDETKFIPSYPT 120
           KYFTFGPP+ FI DFSRRYGK+DMYKTHIFGKPTIIVCKPEICRQV LT+E KFIP YP 
Sbjct: 61  KYFTFGPPESFIADFSRRYGKLDMYKTHIFGKPTIIVCKPEICRQVLLTEEAKFIPGYPI 120

Query: 121 SITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKP 180
           ++T +FG+KSL QVPKEEHRKLRRLT APISGHAALEMYIDHIEHTVISGLEEWS  +KP
Sbjct: 121 TMTTIFGRKSLHQVPKEEHRKLRRLTTAPISGHAALEMYIDHIEHTVISGLEEWSRTEKP 180

Query: 181 LELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKAL 240
           LELLTTIK LT KVIWNIFMGSTPIKSTSIREME LNE+IALAF TLP+NFPG PFHKAL
Sbjct: 181 LELLTTIKHLTLKVIWNIFMGSTPIKSTSIREMENLNEDIALAFFTLPINFPGFPFHKAL 240

Query: 241 MAGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGM 300
           M  KRLNEILQSI+DEKRMVKKSKGENW+AKDMMDLLMEVRDEDGEGFDDETITE+IF M
Sbjct: 241 MGRKRLNEILQSILDEKRMVKKSKGENWKAKDMMDLLMEVRDEDGEGFDDETITEMIFSM 300

Query: 301 LFGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQ 360
           LFGGQET+AFTTMWAVLFLTDNP +FQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+LSQ
Sbjct: 301 LFGGQETSAFTTMWAVLFLTDNPHIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKYLSQ 360

Query: 361 VIDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSR 420
           VIDETLRL S+AF +FREAT D+EINGKIIPKGW+VILWLR L MD+KLHPSPQ FNPSR
Sbjct: 361 VIDETLRLGSIAFATFREATTDVEINGKIIPKGWKVILWLRELYMDEKLHPSPQQFNPSR 420

Query: 421 WD 422
           WD
Sbjct: 421 WD 422

[Close]

BLAST of CSPI03G01910 vs. NCBI nr
Match: XP_008448720.1 (PREDICTED: beta-amyrin 11-oxidase-like [Cucumis melo])

HSP 1 Score: 734.6 bits (1895), Expect = 5.0e-208
Identity = 354/421 (84.09%), Postives = 389/421 (92.40%), Query Frame = 0


Query: 1   MELMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFL 60
           MEL++NWLLLIVPFV FVLGFGVLKRLNNLYYALKL KKWDELPPGDLSWP IGSTLSFL
Sbjct: 1   MELVINWLLLIVPFVGFVLGFGVLKRLNNLYYALKLGKKWDELPPGDLSWPFIGSTLSFL 60

Query: 61  KYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTS 120
           KYFTFGPP+ FI DFSRRYGK+ MYKTHIFG  TIIVCKPEICRQVLTDE+KFIP+Y T+
Sbjct: 61  KYFTFGPPESFIADFSRRYGKVGMYKTHIFGNLTIIVCKPEICRQVLTDESKFIPAYHTT 120

Query: 121 ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPL 180
           +  +FGK+SL QV KEEH+KLRRL   PISGHA+LEMYIDHIE T ISG EEWSSM+KPL
Sbjct: 121 MKTLFGKRSLHQVSKEEHQKLRRLITIPISGHASLEMYIDHIEQTAISGFEEWSSMEKPL 180

Query: 181 ELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALM 240
           ELLT+IKQLTFKVIWNIFMGSTP KST+IREME+LN++I LAF T+P+NFPG  FHKAL 
Sbjct: 181 ELLTSIKQLTFKVIWNIFMGSTPTKSTTIREMESLNDDIVLAFFTMPINFPGFSFHKALK 240

Query: 241 AGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGML 300
           A KRL+EILQSI+DEK+MVKK KGE+WEAKDMMDLLMEVRDEDGEGFDDETITE+IFGML
Sbjct: 241 ARKRLHEILQSILDEKKMVKKRKGESWEAKDMMDLLMEVRDEDGEGFDDETITEMIFGML 300

Query: 301 FGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQV 360
           FGGQET+AFTTMWA+LFLTDNP +FQKAKEEQEDIIRRR STQKG+SLSEIKQMKFLSQV
Sbjct: 301 FGGQETSAFTTMWAILFLTDNPHIFQKAKEEQEDIIRRRVSTQKGISLSEIKQMKFLSQV 360

Query: 361 IDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRW 420
           IDETLRLSS+AF++FREAT D+EINGK IPKGW+VILWLR LNMD+KLHPSPQHFNPSRW
Sbjct: 361 IDETLRLSSIAFVTFREATVDVEINGKTIPKGWKVILWLRELNMDEKLHPSPQHFNPSRW 420

Query: 421 D 422
           D
Sbjct: 421 D 421

[Close]

BLAST of CSPI03G01910 vs. NCBI nr
Match: XP_031738658.1 (beta-amyrin 11-oxidase-like [Cucumis sativus])

HSP 1 Score: 733.4 bits (1892), Expect = 1.1e-207
Identity = 372/421 (88.36%), Postives = 373/421 (88.60%), Query Frame = 0


Query: 1   MELMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFL 60
           MELMLNWLLLIVPFV FVLGFGVLKRLNNLYYALKL KKWDELPPGDLSWPLIGSTLSFL
Sbjct: 1   MELMLNWLLLIVPFVGFVLGFGVLKRLNNLYYALKLGKKWDELPPGDLSWPLIGSTLSFL 60

Query: 61  KYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTS 120
           KYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPT+IVCKPEICRQVLTDETKFIPSYPTS
Sbjct: 61  KYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTLIVCKPEICRQVLTDETKFIPSYPTS 120

Query: 121 ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPL 180
           ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPL
Sbjct: 121 ITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPL 180

Query: 181 ELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALM 240
           ELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALM
Sbjct: 181 ELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALM 240

Query: 241 AGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGML 300
                           RMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGML
Sbjct: 241 ----------------RMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGML 300

Query: 301 FGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQV 360
           FGGQETTAFTTMWAVLFLTDNPQVFQKAK                              V
Sbjct: 301 FGGQETTAFTTMWAVLFLTDNPQVFQKAK------------------------------V 360

Query: 361 IDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRW 420
           IDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRW
Sbjct: 361 IDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRW 375

Query: 421 D 422
           D
Sbjct: 421 D 375

[Close]

BLAST of CSPI03G01910 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 347.4 bits (890), Expect = 1.6e-95
Identity = 182/422 (43.13%), Postives = 269/422 (63.74%), Query Frame = 0


Query: 6   NWLLLIVPFVSFVLG-FG---VLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLK 65
           +W+ +  P +  VLG FG   +++++N   Y   L +    LPPGDL WP IG+ LSFL+
Sbjct: 6   SWIPVWFPLM--VLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLR 65

Query: 66  YFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSI 125
            F    PD F R   +RYG   +YK H+FG P+IIV   + CR+VLTD+  F P +PTS 
Sbjct: 66  AFKTSDPDSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTST 125

Query: 126 TAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLE 185
             + G+KS + +  EEH++LRRLT AP++GH AL  YI +IE  VI+ L++W+ M +  E
Sbjct: 126 MELIGRKSFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FE 185

Query: 186 LLTTIKQLTFKVIWNIFMGSTP--IKSTSIREMETLNENIALAFLTLPLNFPGLPFHKAL 245
            LT +++LTF++I  IF+ S    +     RE   LN  +      + +N PG  +H+AL
Sbjct: 186 FLTHLRKLTFRIIMYIFLSSESENVMDALEREYTALNYGVR----AMAVNIPGFAYHRAL 245

Query: 246 MAGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGM 305
            A K L    QSIV E+R  +K    +   KDM+D L+ V+DEDG+  DDE I +++   
Sbjct: 246 KARKTLVAAFQSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMY 305

Query: 306 LFGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQ 365
           L  G E++  T MWA +FL ++P+V Q+AK EQE I++ R   QKG+SL E ++M+FLSQ
Sbjct: 306 LNAGHESSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQ 365

Query: 366 VIDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSR 422
           V+DETLR+ + +  +FREA  D+E+NG +IPKGW+V+ W R +++D ++ P P+ F+P+R
Sbjct: 366 VVDETLRVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPAR 419

[Close]

BLAST of CSPI03G01910 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 342.4 bits (877), Expect = 5.1e-94
Identity = 182/419 (43.44%), Postives = 270/419 (64.44%), Query Frame = 0


Query: 3   LMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLKY 62
           L+L W  LI+    FVL + VLKR+N   Y  KL +K   LPPGDL WP+IG+  SFL+ 
Sbjct: 6   LILMWFPLII-LGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRA 65

Query: 63  FTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSIT 122
           F    P+ FI+ +  RYG+  +YK H+FG P ++V  PE CR+VLTD+  F   +P S  
Sbjct: 66  FKTSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTM 125

Query: 123 AVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLEL 182
            + G+KS + +  EEH++LRRLT AP++G  AL +YI  IE TV + LE+WS M + +E 
Sbjct: 126 KLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEF 185

Query: 183 LTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMAG 242
           L+ +++LTFKVI  IF+ S         E E  N N  +    + +N PG  +H+AL A 
Sbjct: 186 LSHLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVR--AMGINLPGFAYHRALKAR 245

Query: 243 KRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFG 302
           K+L    QSIV  +R  +K    +   KDM+D L++V+DE+G   DDE I +++   L  
Sbjct: 246 KKLVAAFQSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNA 305

Query: 303 GQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 362
           G E++   TMWA + + ++P + QKAKEEQE I+++RA  QK ++L E ++M +LSQVID
Sbjct: 306 GHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVID 365

Query: 363 ETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRWD 422
           ETLR+ + +  +FREA  D++++G IIPKGW+V+ W R +++D +++P P+ F+PSRW+
Sbjct: 366 ETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 417

[Close]

BLAST of CSPI03G01910 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 342.4 bits (877), Expect = 5.1e-94
Identity = 182/419 (43.44%), Postives = 270/419 (64.44%), Query Frame = 0


Query: 3   LMLNWLLLIVPFVSFVLGFGVLKRLNNLYYALKLRKKWDELPPGDLSWPLIGSTLSFLKY 62
           L+L W  LI+    FVL + VLKR+N   Y  KL +K   LPPGDL WP+IG+  SFL+ 
Sbjct: 6   LILMWFPLII-LGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRA 65

Query: 63  FTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSIT 122
           F    P+ FI+ +  RYG+  +YK H+FG P ++V  PE CR+VLTD+  F   +P S  
Sbjct: 66  FKTSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTM 125

Query: 123 AVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLEL 182
            + G+KS + +  EEH++LRRLT AP++G  AL +YI  IE TV + LE+WS M + +E 
Sbjct: 126 KLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEF 185

Query: 183 LTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMAG 242
           L+ +++LTFKVI  IF+ S         E E  N N  +    + +N PG  +H+AL A 
Sbjct: 186 LSHLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVR--AMGINLPGFAYHRALKAR 245

Query: 243 KRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFG 302
           K+L    QSIV  +R  +K    +   KDM+D L++V+DE+G   DDE I +++   L  
Sbjct: 246 KKLVAAFQSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNA 305

Query: 303 GQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 362
           G E++   TMWA + + ++P + QKAKEEQE I+++RA  QK ++L E ++M +LSQVID
Sbjct: 306 GHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVID 365

Query: 363 ETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRGLNMDQKLHPSPQHFNPSRWD 422
           ETLR+ + +  +FREA  D++++G IIPKGW+V+ W R +++D +++P P+ F+PSRW+
Sbjct: 366 ETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWE 417

[Close]

BLAST of CSPI03G01910 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 200.3 bits (508), Expect = 3.1e-51
Identity = 118/381 (30.97%), Postives = 203/381 (53.28%), Query Frame = 0


Query: 43  LPPGDLSWPLIGSTLSFLKYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEI 102
           LPPG +  P IG T   L+ +T   P+ F      +YG  D++KTHI G P +++  PE 
Sbjct: 49  LPPGSMGLPYIGET---LRLYTEN-PNSFFATRQNKYG--DIFKTHILGCPCVMISSPEA 108

Query: 103 CRQVLTDETK-FIPSYPTSITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDH 162
            R VL  +   F P+YP S   + G ++L       H  L+RL  +     +AL   + H
Sbjct: 109 ARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFM-PSALRPTVSH 168

Query: 163 IEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIAL 222
           IE  V+  L  W+S +K +  L  +K+  F V      G    + T+I  ++ L + +  
Sbjct: 169 IELLVLQTLSSWTS-QKSINTLEYMKRYAFDVAIMSAFGDKE-EPTTIDVIKLLYQRLER 228

Query: 223 AFLTLPLNFPGLPFHKALMAGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRD 282
            + ++PL+ PG  FHK++ A   L+E L+ +++++R   + +G       ++ +L+  +D
Sbjct: 229 GYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAKD 288

Query: 283 EDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRAS 342
           +   G  D  I + I G++F   +TTA    W + +L D+P + Q+   EQ  I ++   
Sbjct: 289 QKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKK 348

Query: 343 TQKGVSLSEIKQMKFLSQVIDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRG 402
             + +S  + ++M   ++VI ETLR +SV   +FREA +D+E +G +IPKGW+V+   R 
Sbjct: 349 ENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRR 408

Query: 403 LNMDQKLHPSPQHFNPSRWDV 423
           ++   +  P P+ F+PSR++V
Sbjct: 409 IHHSSEFFPDPEKFDPSRFEV 414

[Close]

BLAST of CSPI03G01910 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 200.3 bits (508), Expect = 3.1e-51
Identity = 118/381 (30.97%), Postives = 203/381 (53.28%), Query Frame = 0


Query: 43  LPPGDLSWPLIGSTLSFLKYFTFGPPDGFIRDFSRRYGKIDMYKTHIFGKPTIIVCKPEI 102
           LPPG +  P IG T   L+ +T   P+ F      +YG  D++KTHI G P +++  PE 
Sbjct: 49  LPPGSMGLPYIGET---LRLYTEN-PNSFFATRQNKYG--DIFKTHILGCPCVMISSPEA 108

Query: 103 CRQVLTDETK-FIPSYPTSITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDH 162
            R VL  +   F P+YP S   + G ++L       H  L+RL  +     +AL   + H
Sbjct: 109 ARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFM-PSALRPTVSH 168

Query: 163 IEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTPIKSTSIREMETLNENIAL 222
           IE  V+  L  W+S +K +  L  +K+  F V      G    + T+I  ++ L + +  
Sbjct: 169 IELLVLQTLSSWTS-QKSINTLEYMKRYAFDVAIMSAFGDKE-EPTTIDVIKLLYQRLER 228

Query: 223 AFLTLPLNFPGLPFHKALMAGKRLNEILQSIVDEKRMVKKSKGENWEAKDMMDLLMEVRD 282
            + ++PL+ PG  FHK++ A   L+E L+ +++++R   + +G       ++ +L+  +D
Sbjct: 229 GYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAKD 288

Query: 283 EDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPQVFQKAKEEQEDIIRRRAS 342
           +   G  D  I + I G++F   +TTA    W + +L D+P + Q+   EQ  I ++   
Sbjct: 289 QKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKK 348

Query: 343 TQKGVSLSEIKQMKFLSQVIDETLRLSSVAFISFREATEDIEINGKIIPKGWRVILWLRG 402
             + +S  + ++M   ++VI ETLR +SV   +FREA +D+E +G +IPKGW+V+   R 
Sbjct: 349 ENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRR 408

Query: 403 LNMDQKLHPSPQHFNPSRWDV 423
           ++   +  P P+ F+PSR++V
Sbjct: 409 IHHSSEFFPDPEKFDPSRFEV 414

The following BLAST results are available for this feature:

BLAST of CSPI03G01910 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	6.1e-116	48.77	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
O23051	2.2e-94	43.13	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q9C5Y2	7.2e-93	43.44	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q5VRM7	1.2e-90	38.85	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q43246	1.5e-90	41.69	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]

BLAST of CSPI03G01910 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BKZ4	3.5e-215	87.44	beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490804 P...	[more]
A0A1S3BKD3	2.4e-208	84.09	beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103490805 PE=3 SV=1	[more]
A0A5A7UBF0	8.0e-204	84.86	Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1L1P3	3.2e-176	72.66	beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111500300 PE=3 SV=...	[more]
A0A6J1E6E2	4.1e-176	72.42	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111431022 PE=3 S...	[more]

BLAST of CSPI03G01910 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739358.1	3.7e-227	93.59	beta-amyrin 11-oxidase isoform X1 [Cucumis sativus]	[more]
XP_031739359.1	3.7e-227	93.59	beta-amyrin 11-oxidase isoform X2 [Cucumis sativus] >KAE8650036.1 hypothetical p...	[more]
XP_008448717.1	7.2e-215	87.44	PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo]	[more]
XP_008448720.1	5.0e-208	84.09	PREDICTED: beta-amyrin 11-oxidase-like [Cucumis melo]	[more]
XP_031738658.1	1.1e-207	88.36	beta-amyrin 11-oxidase-like [Cucumis sativus]	[more]

BLAST of CSPI03G01910 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05160.1	1.6e-95	43.13	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G32440.1	5.1e-94	43.44	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	5.1e-94	43.44	ent-kaurenoic acid hydroxylase 2	[more]
AT2G29090.1	3.1e-51	30.97	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	3.1e-51	30.97	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 403..421 score: 26.72 coord: 354..370 score: 43.44 coord: 293..319 score: 27.92
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 35..422 e-value: 3.0E-88 score: 298.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 41..420
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 44..421 e-value: 6.4E-58 score: 196.6
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 21..421
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 21..421

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G01910.1	CSPI03G01910.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen