CSPI03G01900 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G01900
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	beta-amyrin 11-oxidase-like
Location	Chr3: 1338342 .. 1341455 (-)
RNA-Seq Expression	CSPI03G01900
Synteny	CSPI03G01900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACAAGACTCACATATTTGGAAAACCAACTATAATCGTCTGCAAACCTGAAATATGTCGTCAAATATTAACTGATGAAACCAAGTTCATTCCTAGTTATCCTACATCCATCACAGCCGTATTTGGTAAAAAATCACTGCTTCAAGTACCGAAAGAGGAGCATCGGAAGCTCCGACGGCTGACAATGGCACCGATCAGTGGCCATGCAGCGCTCGAGATGTACATTGATCATATTGAACACACTGTGATTAGTGGGTTGGAGGAGTGGTCAAGCATGAAGAAGCCATTGGAATTATTAACAACGATAAAGCAACTTACCTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACTCCAATAAAAAGCACTTCTATAGGAGAAATGGAGACTTTGAATGAGAATATTGCACTTGCGTTCCTCACTCTGCCACTCAACTTTCCTGGCTTACCCTTCCATAAAGCACTCATGGTAAGAACAAAAATAATTTCTAATACAAATGTGACGAAGTGTCGATAACTTTTATCATATATGTAAAATATATTTTCAATTAATCTACTCTACAATAATCACTATATCATATGATACAACGAAAAGAAATGTATAAATTATATGTAGGCTGGAAAAAGATTGAATGAAATACTTCAATCATATTTGGATGAGAAGAGAATGGTGAAGAAAAGCAAAGGGGAAAATTGGGAAGCAAAAGATATGATGGATTTGTTGATGGAAGTGAGAGATGAAGATGGTGAAGGATTTGATGATGAGACCATTACAGAGATAATTTTTGGTATGCTATTTGGAGGCCAAGAGACTACAGCCTTCACTACAATGTGGGCAGTTTTGTTTCTTACGGATAATCCACATATATTCCAGAAGGCCAAGGTTAGACTCTTCTTTAATTTCCCAACTCGTGTTTTAATTGCATAATAATGTATATGAGATAAATGAAACTGAATTAAGATATATATGATCAACGATTATACTACAAATTCACATAGATATTTTAGGGTATGCTATTTTGATTTCGAGATTCATATTAAAGAGACGTTATATGAATGTACCAATAATATAGTTGAGATATCCTAGTGTGTATCTATTGATTCTCGTTCTTGGTGTTTTTTTTTTCTGAAAAAAAAAAGCATATTTAATTTGTATTGGTTGTTTGAAAAAATTATCAATATCGACAAAAAGATCGAAACGTTACTTTTGTAAAAATATAAATGAAAAGTTTGGTTAAAATATTTGTGTCGATGGTATGAGATATAAATTGCATAATAATGTGTATGAGATATAAATGAAACTTAATTAAGATATATATGATCAAGATTTAGACTACATATCATATCGAAATTTTAAAGTATGCTATTTTGATTTTGAGATACATGATAAAGAGACGCTATATATATGATGTTTTTCTCGGAAAAAAAAAAGCATATTTGTGTTGGTTGTTTGAAAAATATATCAATACTGACAAAACTATCAAAACACTACTTTTGTAAAAATATGGATGAAAATGTATCGTTGTTAAAATGTTGGTGTCAAGGGACATTACTTAGAAAATTAGAAAAGTAAAAAAATAAAAAACAAATTTAAATTAGTAAATAAACCTATTATTATGATATTTAAACCAGATTAGCATGTTTATTATTATTTATATTACACAATTTATTATATATATATATTGATTCATTTCAAGTCGATGTCAAACTGTTTGAAACGTAAAAAATATTGACATATCAACAGAACATCTGAAAATTTAATACAACAGACATATGATCAAGATATGGATAACATAGTAATCTAGTATTTTTTTTATTAATTTTATTATTATGAAATGAATTATTATTTTGATGGTTAAGTGTAAGATTGGTTTTAGGAAGAACAAGAAGATATCATTAGAAGAAGAGCCTCAACACAAAAGGGTGTAAGTCTGTCAGAGATTAAACAAATGAAATTTCTTTCTCAGGTATTTTAATAATTTTAATTAATTTCCTTTGATTTTTATTTTAATTTTTAGAACATTAATTTGGGTTGGTAATCAATTTTGATTTCAGAATGTTTTTAAATTATTAACATAAAAGAATTAGTATTAAAATTCTAAATGATTAATTGTTGAGTTTTCTTTTATGGTTGTTGATCCGTTCATGATTAAAGACTTGTCCTCTTCCTTAAAATATTCTCTAATTAGTTTGTTAGCACTATTGTTTTAAATATCAATGCTTTTGGTTACCTCTGTTTTCTTTTTTGGGCATTGGACTTATCTCTAGTCCTTTTTGTTCTCTCTTGCTTCTTGTACTCTCCCTTCTTATATTAATGAAGTGGAGATGATGAGGGTGCTAAGGGAGTGTCTACCTAGTGGAGATGTTCGGGTGCACCTATTAACCCGTATCTGTATCTTTTTTAAAATAAAAGACTTGTCCTCTTAACAAATTTATGAAATATAACTTTTTGATTGAGCAATAGAACATTTCCATATTTTTTAAAGATAATCATATGTATCGTAGTCCTAATTAGATCGTGATGCCTATCTATTGCAATTATAGGCTATAAAGATTATGATTTCCCCACTCTATATTCACGTAACCTTACCCCTAATGCATAATGATTGAGATTTTCATGAATGTCCCCTGTATTTGTGGAATATTTTAACAAACTATGATAAAAAAAAAAAAAAATGTCATAGAAATGGTCATCCCTAAGACTAAGTAAAAAAGTTAGACTACATGATTAAAAGTTTGTGAGAGGACGGATCATTGATTTGTATTTATGTTACCAAGAAGTATCTAAATATAGTAACTAGAATTGCTATAGTATATGGCTCAACTAGAAAGGGGCTTTGTAATCAGAGTTTTGAGTTTCTCTCTAGTTTGTTTTGTACATTTCCATTAATCAATGATTTTACTTTTGGTCTTGGGACATGATGAGGATGTTATAAAGAGATATCAACTTAGTTGAGATATCTTGATGTATCTCCTAATCCACTCGTTTTGTATATATTTTCAAAATCTAGTAATATTGAATTATAACTATTAATTCTATATACTTTATGTATAATATTCAGGTTATAGACTAGACGCTTCGCTTAAGTAGTGTGGTATTTGCTGTTTTTCG

mRNA sequence

ATGTACAAGACTCACATATTTGGAAAACCAACTATAATCGTCTGCAAACCTGAAATATGTCGTCAAATATTAACTGATGAAACCAAGTTCATTCCTAGTTATCCTACATCCATCACAGCCGTATTTGGTAAAAAATCACTGCTTCAAGTACCGAAAGAGGAGCATCGGAAGCTCCGACGGCTGACAATGGCACCGATCAGTGGCCATGCAGCGCTCGAGATGTACATTGATCATATTGAACACACTGTGATTAGTGGGTTGGAGGAGTGGTCAAGCATGAAGAAGCCATTGGAATTATTAACAACGATAAAGCAACTTACCTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACTCCAATAAAAAGCACTTCTATAGGAGAAATGGAGACTTTGAATGAGAATATTGCACTTGCGTTCCTCACTCTGCCACTCAACTTTCCTGGCTTACCCTTCCATAAAGCACTCATGGCTGGAAAAAGATTGAATGAAATACTTCAATCATATTTGGATGAGAAGAGAATGGTGAAGAAAAGCAAAGGGGAAAATTGGGAAGCAAAAGATATGATGGATTTGTTGATGGAAGTGAGAGATGAAGATGGTGAAGGATTTGATGATGAGACCATTACAGAGATAATTTTTGGTATGCTATTTGGAGGCCAAGAGACTACAGCCTTCACTACAATGTGGGCAGTTTTGTTTCTTACGGATAATCCACATATATTCCAGAAGGCCAAGGAAGAACAAGAAGATATCATTAGAAGAAGAGCCTCAACACAAAAGGGTGTAAGTCTGTCAGAGATTAAACAAATGAAATTTCTTTCTCAGGTTATAGACTAGACGCTTCGCTTAAGTAGTGTGGTATTTGCTGTTTTTCG

Coding sequence (CDS)

ATGTACAAGACTCACATATTTGGAAAACCAACTATAATCGTCTGCAAACCTGAAATATGTCGTCAAATATTAACTGATGAAACCAAGTTCATTCCTAGTTATCCTACATCCATCACAGCCGTATTTGGTAAAAAATCACTGCTTCAAGTACCGAAAGAGGAGCATCGGAAGCTCCGACGGCTGACAATGGCACCGATCAGTGGCCATGCAGCGCTCGAGATGTACATTGATCATATTGAACACACTGTGATTAGTGGGTTGGAGGAGTGGTCAAGCATGAAGAAGCCATTGGAATTATTAACAACGATAAAGCAACTTACCTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACTCCAATAAAAAGCACTTCTATAGGAGAAATGGAGACTTTGAATGAGAATATTGCACTTGCGTTCCTCACTCTGCCACTCAACTTTCCTGGCTTACCCTTCCATAAAGCACTCATGGCTGGAAAAAGATTGAATGAAATACTTCAATCATATTTGGATGAGAAGAGAATGGTGAAGAAAAGCAAAGGGGAAAATTGGGAAGCAAAAGATATGATGGATTTGTTGATGGAAGTGAGAGATGAAGATGGTGAAGGATTTGATGATGAGACCATTACAGAGATAATTTTTGGTATGCTATTTGGAGGCCAAGAGACTACAGCCTTCACTACAATGTGGGCAGTTTTGTTTCTTACGGATAATCCACATATATTCCAGAAGGCCAAGGAAGAACAAGAAGATATCATTAGAAGAAGAGCCTCAACACAAAAGGGTGTAAGTCTGTCAGAGATTAAACAAATGAAATTTCTTTCTCAGGTTATAGACTAG

Protein sequence

MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTPIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPHIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID*

Homology

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BLAST of CSPI03G01900 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 8.1e-69
Identity = 131/280 (46.79%), Postives = 196/280 (70.00%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YKTH+FG P+IIVC+P++CR++LTD+  F   YP SI  +   + ++ V   EHR  RR
Sbjct: 84  IYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVSNAEHRLFRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           L  +PI GH AL MY++ +E  VI+ LEE SSMK P+ELL  +K+++FK I ++FMGS+ 
Sbjct: 144 LITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVHVFMGSS- 203

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKR-MVKKS 180
                I ++ +   ++     ++P+N PG  FHKAL A K+L +I+Q  +DE+R M++  
Sbjct: 204 -NQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVVDERRLMIENG 263

Query: 181 KGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 240
             E  + KD++D+L+EV+DE+G   +DE I++++ G+LF G E+TA + MW++ +LT +P
Sbjct: 264 PQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLMWSITYLTQHP 323

Query: 241 HIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           HI +KAKEEQE+I R R S+QK +SL EIKQM +LSQVID
Sbjct: 324 HILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVID 361

[Close]

BLAST of CSPI03G01900 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.1e-56
Identity = 117/279 (41.94%), Postives = 175/279 (62.72%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YK H+FG P+IIV   + CR++LTD+  F P +PTS   + G+KS + +  EEH++LRR
Sbjct: 86  IYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRR 145

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT AP++GH AL  YI +IE  VI+ L++W+ M +  E LT +++LTF++I  IF+ S  
Sbjct: 146 LTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSES 205

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
                  E E    N  +    + +N PG  +H+AL A K L    QS + E+R  +K  
Sbjct: 206 ENVMDALEREYTALNYGVR--AMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQN 265

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             +   KDM+D L+ V+DEDG+  DDE I +++   L  G E++  T MWA +FL ++P 
Sbjct: 266 ILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPE 325

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           + Q+AK EQE I++ R   QKG+SL E ++M+FLSQV+D
Sbjct: 326 VLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVD 360

[Close]

BLAST of CSPI03G01900 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 4.8e-53
Identity = 116/279 (41.58%), Postives = 174/279 (62.37%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YK H+FG P ++V  PE CR++LTD+  F   +P S   + G+KS + +  EEH++LRR
Sbjct: 85  IYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRR 144

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT AP++G  AL +YI  IE TV + LE+WS M + +E L+ +++LTFKVI  IF+ S  
Sbjct: 145 LTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFKVIMYIFLSSES 204

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
                  E E  N N  +    + +N PG  +H+AL A K+L    QS +  +R  +K  
Sbjct: 205 EHVMDSLEREYTNLNYGVR--AMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQN 264

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             +   KDM+D L++V+DE+G   DDE I +++   L  G E++   TMWA + + ++P 
Sbjct: 265 ISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPM 324

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           I QKAKEEQE I+++RA  QK ++L E ++M +LSQVID
Sbjct: 325 ILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVID 358

[Close]

BLAST of CSPI03G01900 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 9.0e-52
Identity = 106/279 (37.99%), Postives = 170/279 (60.93%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +Y++ +F  PT++V   E C+Q+L D+  F+  +P +  A+ G +S + +P +EHR++R+
Sbjct: 113 VYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRIRK 172

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT API+G  AL  Y+  I+ TV S L  W+     +E LT ++++TFK+I  IF+G   
Sbjct: 173 LTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGGAD 232

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
             +T   E      N  +    + +N PG  +  AL A +RL  +LQ  LDE+R   ++K
Sbjct: 233 QATTRALERSYTELNYGMR--AMAINLPGFAYRGALRARRRLVAVLQGVLDERR-AARAK 292

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           G +    DMMD L+E +DE G   DD+ I +++   L  G E++   TMWA +FL +NP 
Sbjct: 293 GVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPD 352

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           +F +AK EQE I+R   S+Q+G++L + ++M++LSQVID
Sbjct: 353 MFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVID 388

[Close]

BLAST of CSPI03G01900 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.6e-51
Identity = 103/280 (36.79%), Postives = 166/280 (59.29%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +Y+T +F  PTI+   PE C+Q+L D+  F+  +P +   + G KS + +  ++HR++R+
Sbjct: 96  VYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFVNMSYDDHRRIRK 155

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKK-PLELLTTIKQLTFKVIWNIFMGST 120
           LT API+G  AL  Y+  I+ TV++ L  WSS +   +E LT ++++TFK+I  IFM   
Sbjct: 156 LTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKIIVQIFMSGA 215

Query: 121 PIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKS 180
                ++  +E    ++      + +N PG  +++AL A ++L  +LQ  LD +R     
Sbjct: 216 --DDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQGVLDGRRAAAAK 275

Query: 181 KGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 240
             +   A DMMD L+E  DE G    D+ I +++   L  G E++   TMWA +FL +NP
Sbjct: 276 GFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHITMWATVFLQENP 335

Query: 241 HIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
            IF +AK EQE+I+R   +TQ G++L + K+M FLSQV+D
Sbjct: 336 DIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVD 373

[Close]

BLAST of CSPI03G01900 vs. ExPASy TrEMBL
Match: A0A1S3BKZ4 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490804 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.2e-136
Identity = 247/280 (88.21%), Postives = 261/280 (93.21%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQI-LTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLR 60
           MYKTHIFGKPTIIVCKPEICRQ+ LT+E KFIP YP ++T +FG+KSL QVPKEEHRKLR
Sbjct: 84  MYKTHIFGKPTIIVCKPEICRQVLLTEEAKFIPGYPITMTTIFGRKSLHQVPKEEHRKLR 143

Query: 61  RLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGST 120
           RLT APISGHAALEMYIDHIEHTVISGLEEWS  +KPLELLTTIK LT KVIWNIFMGST
Sbjct: 144 RLTTAPISGHAALEMYIDHIEHTVISGLEEWSRTEKPLELLTTIKHLTLKVIWNIFMGST 203

Query: 121 PIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKS 180
           PIKSTSI EME LNE+IALAF TLP+NFPG PFHKALM  KRLNEILQS LDEKRMVKKS
Sbjct: 204 PIKSTSIREMENLNEDIALAFFTLPINFPGFPFHKALMGRKRLNEILQSILDEKRMVKKS 263

Query: 181 KGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 240
           KGENW+AKDMMDLLMEVRDEDGEGFDDETITE+IF MLFGGQET+AFTTMWAVLFLTDNP
Sbjct: 264 KGENWKAKDMMDLLMEVRDEDGEGFDDETITEMIFSMLFGGQETSAFTTMWAVLFLTDNP 323

Query: 241 HIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           HIFQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+LSQVID
Sbjct: 324 HIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKYLSQVID 363

[Close]

BLAST of CSPI03G01900 vs. ExPASy TrEMBL
Match: A0A5A7UBF0 (Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001820 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.7e-134
Identity = 247/293 (84.30%), Postives = 262/293 (89.42%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQI-LTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLR 60
           MYKTHIFGKPTIIVCKPEICRQ+ LT+E KFIP YP ++T +FG+KSL QVPKEEHRKLR
Sbjct: 67  MYKTHIFGKPTIIVCKPEICRQVLLTEEAKFIPGYPITMTTIFGRKSLHQVPKEEHRKLR 126

Query: 61  RLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGST 120
           RLT APISGHAALEMYIDHIEHTVISGLEEWS  +KPLELLTTIK LT KVIWNIFMGST
Sbjct: 127 RLTTAPISGHAALEMYIDHIEHTVISGLEEWSRTEKPLELLTTIKHLTLKVIWNIFMGST 186

Query: 121 PIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALM-------------AGKRLNEIL 180
           PIKSTSI EME LNE+IALAF TLP+NFPG PFHKALM             + KRLNEIL
Sbjct: 187 PIKSTSIREMENLNEDIALAFFTLPINFPGFPFHKALMERTKIISNTNATKSRKRLNEIL 246

Query: 181 QSYLDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAF 240
           QS LDEKRMVKKSKGENW+AKDMMDLLMEVRDEDGEGFDDETITE+IF MLFGGQET+AF
Sbjct: 247 QSILDEKRMVKKSKGENWKAKDMMDLLMEVRDEDGEGFDDETITEMIFSMLFGGQETSAF 306

Query: 241 TTMWAVLFLTDNPHIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           TTMWAVLFLTDNPHIFQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+LSQVID
Sbjct: 307 TTMWAVLFLTDNPHIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKYLSQVID 359

[Close]

BLAST of CSPI03G01900 vs. ExPASy TrEMBL
Match: A0A0A0L419 (Cytochrome P450 88D6 OS=Cucumis sativus OX=3659 GN=Csa_3G015360 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.4e-132
Identity = 239/279 (85.66%), Postives = 255/279 (91.40%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFGKPTIIVCKPE+CRQ+LTDE KFIP YPT++  +FGKKSL  V KEEHRKLRR
Sbjct: 1   MYKTHIFGKPTIIVCKPELCRQVLTDEAKFIPGYPTTMKTLFGKKSLHAVTKEEHRKLRR 60

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           L   PIS HAALEMYIDHIE TVISGLEEWSSM+KPLELLTTIKQLTFKVIWNIFMGSTP
Sbjct: 61  LITTPISSHAALEMYIDHIERTVISGLEEWSSMEKPLELLTTIKQLTFKVIWNIFMGSTP 120

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
           IKSTSI EMET N++I L F T+P+NFPG  FHKAL+A K L+EILQS LDEKRMVKKSK
Sbjct: 121 IKSTSIREMETFNDDIVLGFFTMPINFPGFSFHKALLARKGLHEILQSILDEKRMVKKSK 180

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GENWEAKDMMDLLMEVRDEDGEGFDDETITE+IF MLFGGQET+AFT+MWAVLFLTDNPH
Sbjct: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEMIFSMLFGGQETSAFTSMWAVLFLTDNPH 240

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           IFQKAKEEQEDII+RRASTQ GVSLSEIKQMKFLSQVID
Sbjct: 241 IFQKAKEEQEDIIKRRASTQTGVSLSEIKQMKFLSQVID 279

[Close]

BLAST of CSPI03G01900 vs. ExPASy TrEMBL
Match: A0A1S3BKD3 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103490805 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 9.5e-129
Identity = 231/279 (82.80%), Postives = 255/279 (91.40%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFG  TIIVCKPEICRQ+LTDE+KFIP+Y T++  +FGK+SL QV KEEH+KLRR
Sbjct: 84  MYKTHIFGNLTIIVCKPEICRQVLTDESKFIPAYHTTMKTLFGKRSLHQVSKEEHQKLRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           L   PISGHA+LEMYIDHIE T ISG EEWSSM+KPLELLT+IKQLTFKVIWNIFMGSTP
Sbjct: 144 LITIPISGHASLEMYIDHIEQTAISGFEEWSSMEKPLELLTSIKQLTFKVIWNIFMGSTP 203

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
            KST+I EME+LN++I LAF T+P+NFPG  FHKAL A KRL+EILQS LDEK+MVKK K
Sbjct: 204 TKSTTIREMESLNDDIVLAFFTMPINFPGFSFHKALKARKRLHEILQSILDEKKMVKKRK 263

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GE+WEAKDMMDLLMEVRDEDGEGFDDETITE+IFGMLFGGQET+AFTTMWA+LFLTDNPH
Sbjct: 264 GESWEAKDMMDLLMEVRDEDGEGFDDETITEMIFGMLFGGQETSAFTTMWAILFLTDNPH 323

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           IFQKAKEEQEDIIRRR STQKG+SLSEIKQMKFLSQVID
Sbjct: 324 IFQKAKEEQEDIIRRRVSTQKGISLSEIKQMKFLSQVID 362

[Close]

BLAST of CSPI03G01900 vs. ExPASy TrEMBL
Match: A0A1S3BL88 (beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490804 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.5e-118
Identity = 218/246 (88.62%), Postives = 230/246 (93.50%), Query Frame = 0


Query: 34  YPTSITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSM 93
           YP ++T +FG+KSL QVPKEEHRKLRRLT APISGHAALEMYIDHIEHTVISGLEEWS  
Sbjct: 39  YPITMTTIFGRKSLHQVPKEEHRKLRRLTTAPISGHAALEMYIDHIEHTVISGLEEWSRT 98

Query: 94  KKPLELLTTIKQLTFKVIWNIFMGSTPIKSTSIGEMETLNENIALAFLTLPLNFPGLPFH 153
           +KPLELLTTIK LT KVIWNIFMGSTPIKSTSI EME LNE+IALAF TLP+NFPG PFH
Sbjct: 99  EKPLELLTTIKHLTLKVIWNIFMGSTPIKSTSIREMENLNEDIALAFFTLPINFPGFPFH 158

Query: 154 KALMAGKRLNEILQSYLDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEII 213
           KALM  KRLNEILQS LDEKRMVKKSKGENW+AKDMMDLLMEVRDEDGEGFDDETITE+I
Sbjct: 159 KALMGRKRLNEILQSILDEKRMVKKSKGENWKAKDMMDLLMEVRDEDGEGFDDETITEMI 218

Query: 214 FGMLFGGQETTAFTTMWAVLFLTDNPHIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKF 273
           F MLFGGQET+AFTTMWAVLFLTDNPHIFQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+
Sbjct: 219 FSMLFGGQETSAFTTMWAVLFLTDNPHIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKY 278

Query: 274 LSQVID 280
           LSQVID
Sbjct: 279 LSQVID 284

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: XP_031739358.1 (beta-amyrin 11-oxidase isoform X1 [Cucumis sativus])

HSP 1 Score: 547.0 bits (1408), Expect = 9.7e-152
Identity = 273/279 (97.85%), Postives = 276/279 (98.92%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFGKPTIIVCKPEICRQ+LTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR
Sbjct: 84  MYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LTMAPISGHAALEMYIDHIEH+VISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP
Sbjct: 144 LTMAPISGHAALEMYIDHIEHSVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 203

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
           IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEI QSYLDEKRMVKKSK
Sbjct: 204 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEIFQSYLDEKRMVKKSK 263

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GENWEAKDMMDLLMEVRDEDGEGFDDETI E+IFGMLFGGQETTAFTTMWAVLFLTDNPH
Sbjct: 264 GENWEAKDMMDLLMEVRDEDGEGFDDETIRELIFGMLFGGQETTAFTTMWAVLFLTDNPH 323

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           IFQKAKEEQEDIIRRRASTQKGVS SEIKQMKFLSQVID
Sbjct: 324 IFQKAKEEQEDIIRRRASTQKGVSFSEIKQMKFLSQVID 362

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: XP_031739359.1 (beta-amyrin 11-oxidase isoform X2 [Cucumis sativus] >KAE8650036.1 hypothetical protein Csa_009692 [Cucumis sativus])

HSP 1 Score: 547.0 bits (1408), Expect = 9.7e-152
Identity = 273/279 (97.85%), Postives = 276/279 (98.92%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFGKPTIIVCKPEICRQ+LTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR
Sbjct: 84  MYKTHIFGKPTIIVCKPEICRQVLTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LTMAPISGHAALEMYIDHIEH+VISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP
Sbjct: 144 LTMAPISGHAALEMYIDHIEHSVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 203

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
           IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEI QSYLDEKRMVKKSK
Sbjct: 204 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEIFQSYLDEKRMVKKSK 263

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GENWEAKDMMDLLMEVRDEDGEGFDDETI E+IFGMLFGGQETTAFTTMWAVLFLTDNPH
Sbjct: 264 GENWEAKDMMDLLMEVRDEDGEGFDDETIRELIFGMLFGGQETTAFTTMWAVLFLTDNPH 323

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           IFQKAKEEQEDIIRRRASTQKGVS SEIKQMKFLSQVID
Sbjct: 324 IFQKAKEEQEDIIRRRASTQKGVSFSEIKQMKFLSQVID 362

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: XP_008448717.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 495.7 bits (1275), Expect = 2.6e-136
Identity = 247/280 (88.21%), Postives = 261/280 (93.21%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQI-LTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLR 60
           MYKTHIFGKPTIIVCKPEICRQ+ LT+E KFIP YP ++T +FG+KSL QVPKEEHRKLR
Sbjct: 84  MYKTHIFGKPTIIVCKPEICRQVLLTEEAKFIPGYPITMTTIFGRKSLHQVPKEEHRKLR 143

Query: 61  RLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGST 120
           RLT APISGHAALEMYIDHIEHTVISGLEEWS  +KPLELLTTIK LT KVIWNIFMGST
Sbjct: 144 RLTTAPISGHAALEMYIDHIEHTVISGLEEWSRTEKPLELLTTIKHLTLKVIWNIFMGST 203

Query: 121 PIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKS 180
           PIKSTSI EME LNE+IALAF TLP+NFPG PFHKALM  KRLNEILQS LDEKRMVKKS
Sbjct: 204 PIKSTSIREMENLNEDIALAFFTLPINFPGFPFHKALMGRKRLNEILQSILDEKRMVKKS 263

Query: 181 KGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 240
           KGENW+AKDMMDLLMEVRDEDGEGFDDETITE+IF MLFGGQET+AFTTMWAVLFLTDNP
Sbjct: 264 KGENWKAKDMMDLLMEVRDEDGEGFDDETITEMIFSMLFGGQETSAFTTMWAVLFLTDNP 323

Query: 241 HIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           HIFQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+LSQVID
Sbjct: 324 HIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKYLSQVID 363

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: KAA0053062.1 (beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa] >TYK11517.1 beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 486.9 bits (1252), Expect = 1.2e-133
Identity = 247/293 (84.30%), Postives = 262/293 (89.42%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQI-LTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLR 60
           MYKTHIFGKPTIIVCKPEICRQ+ LT+E KFIP YP ++T +FG+KSL QVPKEEHRKLR
Sbjct: 67  MYKTHIFGKPTIIVCKPEICRQVLLTEEAKFIPGYPITMTTIFGRKSLHQVPKEEHRKLR 126

Query: 61  RLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGST 120
           RLT APISGHAALEMYIDHIEHTVISGLEEWS  +KPLELLTTIK LT KVIWNIFMGST
Sbjct: 127 RLTTAPISGHAALEMYIDHIEHTVISGLEEWSRTEKPLELLTTIKHLTLKVIWNIFMGST 186

Query: 121 PIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALM-------------AGKRLNEIL 180
           PIKSTSI EME LNE+IALAF TLP+NFPG PFHKALM             + KRLNEIL
Sbjct: 187 PIKSTSIREMENLNEDIALAFFTLPINFPGFPFHKALMERTKIISNTNATKSRKRLNEIL 246

Query: 181 QSYLDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAF 240
           QS LDEKRMVKKSKGENW+AKDMMDLLMEVRDEDGEGFDDETITE+IF MLFGGQET+AF
Sbjct: 247 QSILDEKRMVKKSKGENWKAKDMMDLLMEVRDEDGEGFDDETITEMIFSMLFGGQETSAF 306

Query: 241 TTMWAVLFLTDNPHIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           TTMWAVLFLTDNPHIFQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+LSQVID
Sbjct: 307 TTMWAVLFLTDNPHIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKYLSQVID 359

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: KAE8650038.1 (hypothetical protein Csa_010549 [Cucumis sativus])

HSP 1 Score: 483.8 bits (1244), Expect = 1.0e-132
Identity = 240/252 (95.24%), Postives = 245/252 (97.22%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFGKPT+IVCKPEICRQ+LTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR
Sbjct: 84  MYKTHIFGKPTLIVCKPEICRQVLTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP
Sbjct: 144 LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 203

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
           IKSTSI EMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQS +DEKRMVKKSK
Sbjct: 204 IKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSIVDEKRMVKKSK 263

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 
Sbjct: 264 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPQ 323

Query: 241 IFQKAKEEQEDI 253
           +FQKAK   E +
Sbjct: 324 VFQKAKVIDETL 335

[Close]

BLAST of CSPI03G01900 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 220.7 bits (561), Expect = 1.5e-57
Identity = 117/279 (41.94%), Postives = 175/279 (62.72%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YK H+FG P+IIV   + CR++LTD+  F P +PTS   + G+KS + +  EEH++LRR
Sbjct: 86  IYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRR 145

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT AP++GH AL  YI +IE  VI+ L++W+ M +  E LT +++LTF++I  IF+ S  
Sbjct: 146 LTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSES 205

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
                  E E    N  +    + +N PG  +H+AL A K L    QS + E+R  +K  
Sbjct: 206 ENVMDALEREYTALNYGVR--AMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQN 265

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             +   KDM+D L+ V+DEDG+  DDE I +++   L  G E++  T MWA +FL ++P 
Sbjct: 266 ILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPE 325

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           + Q+AK EQE I++ R   QKG+SL E ++M+FLSQV+D
Sbjct: 326 VLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVD 360

[Close]

BLAST of CSPI03G01900 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 209.5 bits (532), Expect = 3.4e-54
Identity = 116/279 (41.58%), Postives = 174/279 (62.37%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YK H+FG P ++V  PE CR++LTD+  F   +P S   + G+KS + +  EEH++LRR
Sbjct: 85  IYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRR 144

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT AP++G  AL +YI  IE TV + LE+WS M + +E L+ +++LTFKVI  IF+ S  
Sbjct: 145 LTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFKVIMYIFLSSES 204

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
                  E E  N N  +    + +N PG  +H+AL A K+L    QS +  +R  +K  
Sbjct: 205 EHVMDSLEREYTNLNYGVR--AMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQN 264

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             +   KDM+D L++V+DE+G   DDE I +++   L  G E++   TMWA + + ++P 
Sbjct: 265 ISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPM 324

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           I QKAKEEQE I+++RA  QK ++L E ++M +LSQVID
Sbjct: 325 ILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVID 358

[Close]

BLAST of CSPI03G01900 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 209.5 bits (532), Expect = 3.4e-54
Identity = 116/279 (41.58%), Postives = 174/279 (62.37%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YK H+FG P ++V  PE CR++LTD+  F   +P S   + G+KS + +  EEH++LRR
Sbjct: 85  IYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRR 144

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT AP++G  AL +YI  IE TV + LE+WS M + +E L+ +++LTFKVI  IF+ S  
Sbjct: 145 LTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFKVIMYIFLSSES 204

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
                  E E  N N  +    + +N PG  +H+AL A K+L    QS +  +R  +K  
Sbjct: 205 EHVMDSLEREYTNLNYGVR--AMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQN 264

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             +   KDM+D L++V+DE+G   DDE I +++   L  G E++   TMWA + + ++P 
Sbjct: 265 ISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPM 324

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           I QKAKEEQE I+++RA  QK ++L E ++M +LSQVID
Sbjct: 325 ILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVID 358

[Close]

BLAST of CSPI03G01900 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-23
Identity = 71/283 (25.09%), Postives = 134/283 (47.35%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPS-YPTSITAVFGKKSLLQVPKEEHRKLR 60
           ++KT++ G+P I+    ++   +   E +   S YP + T +FGKK++  +    ++ L+
Sbjct: 70  IFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSLHGFMYKYLK 129

Query: 61  RLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGST 120
            + +  + GH  L+  +  +E T    LE WS+ +  +EL      + F +     +   
Sbjct: 130 NMVLT-LFGHDGLKKMLPQVEMTANKRLELWSN-QDSVELKDATASMIFDLTAKKLISHD 189

Query: 121 PIKSTSIGEMETLNENIAL---AFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMV 180
           P KS+     E L  N        ++ P + PG  +HK L    +  ++L++ L E+R  
Sbjct: 190 PDKSS-----ENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERR-- 249

Query: 181 KKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLT 240
              +       D  D ++E   ++G    +E   +++F +LF   ETT+     A+ FL+
Sbjct: 250 ---ENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLS 309

Query: 241 DNPHIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           D+P + ++  EE E I+R R     G++  E K M +  Q I+
Sbjct: 310 DDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFIN 340

[Close]

BLAST of CSPI03G01900 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-23
Identity = 71/283 (25.09%), Postives = 134/283 (47.35%), Query Frame = 0


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPS-YPTSITAVFGKKSLLQVPKEEHRKLR 60
           ++KT++ G+P I+    ++   +   E +   S YP + T +FGKK++  +    ++ L+
Sbjct: 76  IFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSLHGFMYKYLK 135

Query: 61  RLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGST 120
            + +  + GH  L+  +  +E T    LE WS+ +  +EL      + F +     +   
Sbjct: 136 NMVLT-LFGHDGLKKMLPQVEMTANKRLELWSN-QDSVELKDATASMIFDLTAKKLISHD 195

Query: 121 PIKSTSIGEMETLNENIAL---AFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMV 180
           P KS+     E L  N        ++ P + PG  +HK L    +  ++L++ L E+R  
Sbjct: 196 PDKSS-----ENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERR-- 255

Query: 181 KKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLT 240
              +       D  D ++E   ++G    +E   +++F +LF   ETT+     A+ FL+
Sbjct: 256 ---ENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLS 315

Query: 241 DNPHIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           D+P + ++  EE E I+R R     G++  E K M +  Q I+
Sbjct: 316 DDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFIN 346

The following BLAST results are available for this feature:

BLAST of CSPI03G01900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	8.1e-69	46.79	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
O23051	2.1e-56	41.94	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q9C5Y2	4.8e-53	41.58	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q43246	9.0e-52	37.99	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]
Q5VRM7	2.6e-51	36.79	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]

BLAST of CSPI03G01900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BKZ4	1.2e-136	88.21	beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490804 P...	[more]
A0A5A7UBF0	5.7e-134	84.30	Beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0L419	2.4e-132	85.66	Cytochrome P450 88D6 OS=Cucumis sativus OX=3659 GN=Csa_3G015360 PE=4 SV=1	[more]
A0A1S3BKD3	9.5e-129	82.80	beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103490805 PE=3 SV=1	[more]
A0A1S3BL88	1.5e-118	88.62	beta-amyrin 11-oxidase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490804 P...	[more]

BLAST of CSPI03G01900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739358.1	9.7e-152	97.85	beta-amyrin 11-oxidase isoform X1 [Cucumis sativus]	[more]
XP_031739359.1	9.7e-152	97.85	beta-amyrin 11-oxidase isoform X2 [Cucumis sativus] >KAE8650036.1 hypothetical p...	[more]
XP_008448717.1	2.6e-136	88.21	PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo]	[more]
KAA0053062.1	1.2e-133	84.30	beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo var. makuwa] >TYK11517.1 be...	[more]
KAE8650038.1	1.0e-132	95.24	hypothetical protein Csa_010549 [Cucumis sativus]	[more]

BLAST of CSPI03G01900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05160.1	1.5e-57	41.94	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G32440.1	3.4e-54	41.58	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	3.4e-54	41.58	ent-kaurenoic acid hydroxylase 2	[more]
AT1G12740.1	1.8e-23	25.09	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT1G12740.2	1.8e-23	25.09	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..278 e-value: 1.2E-30 score: 106.7
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..279 e-value: 3.3E-53 score: 183.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..278
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 1..279
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 1..279

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G01900.1	CSPI03G01900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen