CSPI03G01250 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G01250
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionvacuolar protein sorting-associated protein 25
LocationChr3: 874611 .. 878797 (+)
RNA-Seq ExpressionCSPI03G01250
SyntenyCSPI03G01250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCTAATGGGTGAGGTTAGAATCCTAAGCAAACGGTCGAGGATAAACATGTATTTGAATTTTATGAATTTATGGATAAAATATCTAACATCCACAAATATTTTTTAAAGTCACAAAATTGCTTCAACAATGAGAGTTTAGATAAAAGCTTTGAAAAACGAATATTTTCTTCCTTCGTTAATGTTCGTTGTCTACCCGGCAAAAAGAAAACCCTCACCTTAGTCTCCTTCTTCCTCACTCTCGCAACGTTCACTCCCCGCGAATACCGAGTGGCACGAACTCCGGTTGACGGCAATCGAACGGCGGCGACAAACACGATTTAGGTATTATTCATTTGTTCCTTTACCCTCTCTTCACTTCCATCTACGATCGCTCAGCGTTGTTTGATCTGAATATCGAAACACGCCATTTGAAGATTGTGATCACACGGGAACTCTGGAACTTCAGCGTTTTCGACATTCAGCTGAATCAAGATCCTGTGAAGGATTTGGAAGTCGATTCTTGAGTTTTGATTTATCAGTTTATCGTTTTCTCTTACCCTGAAATCAGGCGGTCAATCATTTCGAACACGGCTGGATTTGATTACTGCACTTTTTTAACTTTGAAAATTTGAGTCACTAAATCTCGTTTTCTCTGCGCATCGTTGGTTCTGTGTCTTAATTTTTCATTTTCAATCATTAGATCGATGATTATGATTTTCTTACAGATGTAATAATGGGATTGGGATGACTTTCGAATCATGGAGTGTGTTTTGTTTGTAGTGTTTTGATTAGAGGATTTAAAGTGATCATGGATATTCTAAAACATTTGAGTAGTTTAGGAGTAGTTGTTCAATTTCTTCCAAAAATGTTCTTTACAGCACTTTTTTCAATTTCAAATCCTCCTGAAGTTCTGTGAAATCTTGCCTCGATTAATTTCACAAGCCATTTTACTGTAGGCAATAGCATGCTAGTCATGAAGTTGTTCAGTGTTTCTTCAACAATTTTGCATGTTTAAGTAGTTCCTCAGCTCCTATTTGATAGCCAGTTCAATCTCTTGGTTTGGTTTTTTATCATTGAAAATTAAACTTATAGTCACTTCTTTTACTTTTGGATTTCTTTGGTTTGTTATATTCCTTTTACAAATGTTTTCAAACCCAAGTCAAAGCAAAAACTTGGTTTTGTTTTTTGAATTGAACTACCATGTAAAGAAATGGGGGAGAAATTAGGCGCAATTTTCAAGAACCAAATGGATCAAATGGGGTTCTTTTACATGTTAACTATATAGACCCCAAGAGGTGGTGCTCAATCATGGATCTCTTGTCTTCCAACAAAGTCAGACTTGATATTATGGTCTTTTTCAGTCTTGTATCATGTTACTGTGAAGTTTTCATGCCATTTGATGTTTTTTAGAAATAGCTTCATCAGATGAAATTTATTGTTCGGTTCTCACCACTGATCTGATTCATAGAACACGAAAACTTGATTCTATTTTGACGTGTTCGTTTGCTATCATAATCGGGTACAGGATAAAATTAGAAGAGGAAAGCAACTTGCAGTTCATATAGTTCTTTAATGACAAGGTTTTGTTAGTTTGCAAGTCTCTAGTGTCATCGTATCATTTATGAATCTCTGCGTTCTTTTGTCTCTTCATTTTATCTTTAAATTTCTTTCTGAAATTTCTCAGTTCTAGAGATAAGATGCTTCCATAGAACTCAACTCACTTCCAGAGGACACAATAGAGAAGATGCAGAAATTGGGAGATTTCAAGCTACCGCATTTCTTCAATTATCCTCCATATTTCACGTAATGTTCACCATCTGTTTCATTTATTATTCTTTTTACATCATTATTAGCAACTTGTAAGCTGTGTGAGTCAATTTCTGATGATGTTGATATCTTGGTTTTGTCGACAATTTCCTAAGTGAAGAAAACTAACAGGATACAGTATTTACAACCTATAATTTTCCCACCAGCCTCATATATACGAATGTGCTAGTTGTACCTTACCGCAATGTTGATTGTTTCACTTGTCTTCAGTTGCATATATAACCTTTTTATTGCATGTCATCTCAGTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTTGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTTGAAGAGGACTTTCCTCTATTTTCAAATTCTGTGATTGAAAGTAAATTTCATGGCCTTTTTTGGTCCTAATTGGGTGTTAAAACCTTTTTGTGTTTTAGACTCAGTTGCATAGCTTTGTATTACTGTTTCAGTTGTTTGATGTATTTTATAGCGTGGTTGTGATGCTACAGGATCTCTCAGTCATGAAGCTAGACAAGCTTTTATCTCAGCTTTAGTTTCAGATGGTTAGGGCCTGCTTGTTTAGTTCGATAATTCTATCTGTTTACAGTTCCCATTCACTTTCATGGGGAAAAAAACTGATACGTGTTCATTTTAATTGTCCTTATAGGTCGTGCAGAATGGCTGGATAAAGGACATAGGAAATGCCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTAAGACTAATTACTTTGGTTAAATTAGTCTCAAATTTTTGGTCTATTTGTCCTTGAACTTTAAAAAATGTCCAATAGGTCTAGTTCAATTCTAAGTTTCATAGATCCTTGAAAATTTTGAAAATGTTAAAAATTATGCGATCCATTAGGTGTAAATTTGGATTTTATGCTAATGGAACTCTAAATCAAAGGTTCGGAGTCTAAATTTGTAAATTTGAAAATTCAAGGTCCAAATAGGCACAAGTTTCAAAAGTTAAATTAAGAGACTGAATTTATTACTTAACCAAATACCATTTTAACTGCCATAGTTATTAGTAAGTTTGAAATGATTTTGAAAGAGTAAAAATTGCTTAATTCCATTGAAAGAAGTAGGTAAAATTCCACAAGTTTTTTGATAGAAATCAGAAGTGCTTTGAAAATTCGTTATACTTAAATTGTTTCTTAATAAATAGGTGAAAAATAATGGACTTGAAGATAGTGTTATGACTGTCGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGGTACTTTCTTTGCTTATGTTCTCAAGGTACTAAATGCCCACCTTTCGATGATAATTAATGGAATGAAATAATGAGATTAAACTGCTGATCATTTCACTTTTTAAATATGTTAATATATTTTATTTATTTTATGTTCTCGTTAGTGCCATTTTATATGTTTCTGAATGTGTTCCCAAAGGTGATATTAAAACATTTATTGCACACTGTATAGCTTTTGATAGAATAAGGATATTCTTGAAACAAGGGACAGTTCATAGGTTATCTTTTTTAGCCAATTTGTGTATACAAATTCATTAGCTCAGCCATAATTGACATGTAATATCTTCATACCTCACATTTGTCGCGTTAAACGTTGTTAGAAACGTAACGTATAAGTAGAGTGATGATGTATATTTGTTATTTGCCACGAGCACAGCGTAGCTATAGTTGACATGTACTTCTCTTTGAGCCCCCCAGGCCATACCTTACTTTTTGTTGAACTAAAACTTAATGTAAAAGATTTTACGTAAACATTTCCTCACCAGATCTGTTGTAGAGACAATACATGCTTGCTTTCTGCAAAACTACGTAGCTGTTTTGTGCTTAAACATTACCATATCTTGAAACAGCAATTTTGCTTCATTATAATTTGTAAGAAAAGAGTTATTTGGTTTTGTGTTTTATAAATCAGAGCTTGAAGGGATTGATCGTACGATATTAATGAGAGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAATTTTCAAAGGTTCTTCGACAGATGATGAAGGCATTAAATTCTCTGTATAAACTACCAATGTTTATTATTCACATTGACTGCCAATCTTTCTTTTTTCAACACTTCTAAATGCAACTTTTCATTTAGTTTCCCCCCAATTCCAACTGGGATTGATGGGATTATAGGTTCTTGGTGCAGTTCATTGAATGTAACCAAGTGGACCTCCCAATTGAACTTAAAAAGACCTAAGCTTGGGAGTGAGGATTAAAATATTGACGTCGATATTGATGTTAAGGTCTTAAACTTACAGATATATTGACCCAGCGACATATATTTTCATCCTTTTTTCGAGAGTGCTTTGGAAAATA

mRNA sequence

TGCTAATGGGTGAGGTTAGAATCCTAAGCAAACGGTCGAGGATAAACATGTATTTGAATTTTATGAATTTATGGATAAAATATCTAACATCCACAAATATTTTTTAAAGTCACAAAATTGCTTCAACAATGAGAGTTTAGATAAAAGCTTTGAAAAACGAATATTTTCTTCCTTCGTTAATGTTCGTTGTCTACCCGGCAAAAAGAAAACCCTCACCTTAGTCTCCTTCTTCCTCACTCTCGCAACGTTCACTCCCCGCGAATACCGAGTGGCACGAACTCCGGTTGACGGCAATCGAACGGCGGCGACAAACACGATTTAGTTCTAGAGATAAGATGCTTCCATAGAACTCAACTCACTTCCAGAGGACACAATAGAGAAGATGCAGAAATTGGGAGATTTCAAGCTACCGCATTTCTTCAATTATCCTCCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTTGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTTGAAGAGGACTTTCCTCTATTTTCAAATTCTGTGATTGAAAGATCTCTCAGTCATGAAGCTAGACAAGCTTTTATCTCAGCTTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAAGGACATAGGAAATGCCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAATAATGGACTTGAAGATAGTGTTATGACTGTCGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTGAAGGGATTGATCGTACGATATTAATGAGAGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAATTTTCAAAGGTTCTTCGACAGATGATGAAGGCATTAAATTCTCTGTATAAACTACCAATGTTTATTATTCACATTGACTGCCAATCTTTCTTTTTTCAACACTTCTAAATGCAACTTTTCATTTAGTTTCCCCCCAATTCCAACTGGGATTGATGGGATTATAGGTTCTTGGTGCAGTTCATTGAATGTAACCAAGTGGACCTCCCAATTGAACTTAAAAAGACCTAAGCTTGGGAGTGAGGATTAAAATATTGACGTCGATATTGATGTTAAGGTCTTAAACTTACAGATATATTGACCCAGCGACATATATTTTCATCCTTTTTTCGAGAGTGCTTTGGAAAATA

Coding sequence (CDS)

ATGCAGAAATTGGGAGATTTCAAGCTACCGCATTTCTTCAATTATCCTCCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTTGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTTGAAGAGGACTTTCCTCTATTTTCAAATTCTGTGATTGAAAGATCTCTCAGTCATGAAGCTAGACAAGCTTTTATCTCAGCTTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAAGGACATAGGAAATGCCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAATAATGGACTTGAAGATAGTGTTATGACTGTCGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTGAAGGGATTGATCGTACGATATTAATGAGAGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAATTTTCAAAGGTTCTTCGACAGATGATGAAGGCATTAAATTCTCTGTATAA

Protein sequence

MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLFSNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV*
Homology
[Close]
BLAST of CSPI03G01250 vs. ExPASy Swiss-Prot
Match: Q8VZC9 (Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 GN=VPS25 PE=1 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 4.5e-89
Identity = 153/179 (85.47%), Postives = 169/179 (94.41%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNS I+RSLSHEAR+ F+SA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ES GTEL+GIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]
BLAST of CSPI03G01250 vs. ExPASy Swiss-Prot
Match: Q6AX45 (Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps25 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.5e-34
Identity = 69/173 (39.88%), Postives = 106/173 (61.27%), Query Frame = 0 

Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--DFPLFSNSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR  K++ + L E  + PLF+N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLSAWSSLVLSYCRQNKLYTMNLMEIQESPLFNNKK 62

Query: 67  IERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           I+R LS E+ Q  +  L   G  EW+DK   + LI+W R  +W  +I  +V  NG+ +SV
Sbjct: 63  IQRKLSLESVQVVLEELRKKGNLEWIDKNKSRFLIMWRRPDEWGKVIYQWVSKNGMTNSV 122

Query: 127 MTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++ G E  G+D  +L+R+L+ L+Q+ K  I   +  +  G+KF
Sbjct: 123 FTLYELISGDDTEGEEFHGLDEAMLLRSLEALQQEHKAEII--TLNESRGVKF 173

[Close]
BLAST of CSPI03G01250 vs. ExPASy Swiss-Prot
Match: Q6NWF4 (Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 PE=2 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 1.6e-33
Identity = 69/173 (39.88%), Postives = 107/173 (61.85%), Query Frame = 0 

Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--DFPLFSNSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR +K++ + + E  + P+F+N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLAAWCSLVLSYCRHRKLYTLDVLEAQESPVFNNKK 62

Query: 67  IERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           IER LS EA Q     L   G  EWLDK   +CLI+W R ++W  +I  +V  NG+ +SV
Sbjct: 63  IERKLSVEAIQVVFEELRKKGNLEWLDKNKSRCLIMWRRPEEWGKLIYQWVSKNGMVNSV 122

Query: 127 MTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ +G ++   E  G++  +L+R+L+ L+  GK  I   +  D +G+KF
Sbjct: 123 FTLYELANGDDTEKEEFHGLEDWMLLRSLQALQTDGKAEII--TMDDGKGVKF 173

[Close]
BLAST of CSPI03G01250 vs. ExPASy Swiss-Prot
Match: Q55GD9 (Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44689 GN=vps25 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.9e-31
Identity = 69/188 (36.70%), Postives = 116/188 (61.70%), Query Frame = 0 

Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFP----LFSN 66
           F+ P +++  P+FT+QP+ +TR+KQ Q+W++LIL YCR  KI+ + + E       LF+N
Sbjct: 6   FQFPPYYHKEPFFTIQPILNTRKKQFQMWQDLILQYCRYYKIYELDINESIKSNSVLFNN 65

Query: 67  SVIERSLSHEARQAFISALVSDGRAEWLDK-------------GHRKCLILWHRIQDWAD 126
             I R LS EA ++ I  ++ +G AEW+DK              + + LI+W +  +WA 
Sbjct: 66  EKINRKLSREALKSIIDDIIENGFAEWVDKEKEKEKEKDKDNNNNNRVLIMWRKPDEWAS 125

Query: 127 IILHFVKNNGLEDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSS 178
           +I  +V + GL ++V+TV EI++G +S+  E   ++ TILM++LK+LE++ K   F  S 
Sbjct: 126 LIYKWVADCGLLNTVLTVWEIQNGDDSKKQEFHQLNTTILMKSLKVLEKQSKCQTF--SQ 185

[Close]
BLAST of CSPI03G01250 vs. ExPASy Swiss-Prot
Match: Q9CQ80 (Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 6.9e-29
Identity = 64/173 (36.99%), Postives = 99/173 (57.23%), Query Frame = 0 

Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCR--TQKIFVIGLEEDFPLFSNSV 66
           F+ P  + +PP+FTLQP  DTR+KQ+  W  L+L +CR   Q    +   ++ PLF+N  
Sbjct: 5   FEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVK 64

Query: 67  IERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           ++R L  E+ Q  +  L   G  EWLDK     LI+W R ++W  +I  +V  +G  +SV
Sbjct: 65  LQRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSV 124

Query: 127 MTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++   E  G+D   L+RAL+ L+Q+ K  I   + +D  G+KF
Sbjct: 125 FTLYELTSGEDTEDEEFHGLDEATLLRALQALQQEHKAEII--TVSDGRGVKF 175

[Close]
BLAST of CSPI03G01250 vs. ExPASy TrEMBL
Match: A0A0A0L1R7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.3e-98
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. ExPASy TrEMBL
Match: A0A5A7UHJ5 (Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001240 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.6e-97
Identity = 177/179 (98.88%), Postives = 179/179 (100.00%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEARQAF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. ExPASy TrEMBL
Match: A0A1S3BKR8 (vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490745 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.6e-97
Identity = 177/179 (98.88%), Postives = 179/179 (100.00%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEARQAF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. ExPASy TrEMBL
Match: A0A6J1L2J9 (vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LOC111498518 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 3.4e-95
Identity = 173/179 (96.65%), Postives = 177/179 (98.88%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEAR AF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. ExPASy TrEMBL
Match: A0A6J1GDD3 (vacuolar protein sorting-associated protein 25 OS=Cucurbita moschata OX=3662 GN=LOC111452977 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 3.4e-95
Identity = 173/179 (96.65%), Postives = 177/179 (98.88%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEAR AF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. NCBI nr
Match: XP_004146105.1 (vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hypothetical protein Csa_009640 [Cucumis sativus])

HSP 1 Score: 367.1 bits (941), Expect = 8.8e-98
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. NCBI nr
Match: XP_008448627.1 (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo] >KAA0053011.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa] >TYK11467.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 365.2 bits (936), Expect = 3.3e-97
Identity = 177/179 (98.88%), Postives = 179/179 (100.00%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEARQAF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. NCBI nr
Match: XP_038891553.1 (vacuolar protein sorting-associated protein 25 [Benincasa hispida])

HSP 1 Score: 360.9 bits (925), Expect = 6.3e-96
Identity = 173/179 (96.65%), Postives = 178/179 (99.44%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNS IERSLSHEAR+AF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSEIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMT+EEIRSGVESRGTEL+GIDRT+LMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTIEEIRSGVESRGTELQGIDRTVLMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. NCBI nr
Match: XP_022949655.1 (vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_023005573.1 vacuolar protein sorting-associated protein 25 [Cucurbita maxima] >KAG6596709.1 Vacuolar protein sorting-associated protein 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 357.5 bits (916), Expect = 7.0e-95
Identity = 173/179 (96.65%), Postives = 177/179 (98.88%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNSVIERSLSHEAR AF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. NCBI nr
Match: XP_022145259.1 (vacuolar protein sorting-associated protein 25 [Momordica charantia])

HSP 1 Score: 356.7 bits (914), Expect = 1.2e-94
Identity = 170/179 (94.97%), Postives = 178/179 (99.44%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+ IERSLSHEAR+AF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]
BLAST of CSPI03G01250 vs. TAIR 10
Match: AT4G19003.2 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 328.6 bits (841), Expect = 3.2e-90
Identity = 153/179 (85.47%), Postives = 169/179 (94.41%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNS I+RSLSHEAR+ F+SA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ES GTEL+GIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]
BLAST of CSPI03G01250 vs. TAIR 10
Match: AT4G19003.1 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 328.6 bits (841), Expect = 3.2e-90
Identity = 153/179 (85.47%), Postives = 169/179 (94.41%), Query Frame = 0 

Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           SNS I+RSLSHEAR+ F+SA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ES GTEL+GIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

The following BLAST results are available for this feature:
BLAST of CSPI03G01250 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8VZC94.5e-8985.47Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 G... [more]
Q6AX452.5e-3439.88Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps2... [more]
Q6NWF41.6e-3339.88Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 P... [more]
Q55GD91.9e-3136.70Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44... [more]
Q9CQ806.9e-2936.99Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25... [more]
BLAST of CSPI03G01250 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L1R74.3e-98100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=3 SV=1[more]
A0A5A7UHJ51.6e-9798.88Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. m... [more]
A0A1S3BKR81.6e-9798.88vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=365... [more]
A0A6J1L2J93.4e-9596.65vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GDD33.4e-9596.65vacuolar protein sorting-associated protein 25 OS=Cucurbita moschata OX=3662 GN=... [more]
BLAST of CSPI03G01250 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004146105.18.8e-98100.00vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hyp... [more]
XP_008448627.13.3e-9798.88PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis me... [more]
XP_038891553.16.3e-9696.65vacuolar protein sorting-associated protein 25 [Benincasa hispida][more]
XP_022949655.17.0e-9596.65vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_02300557... [more]
XP_022145259.11.2e-9494.97vacuolar protein sorting-associated protein 25 [Momordica charantia][more]
BLAST of CSPI03G01250 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G19003.23.2e-9085.47E2F/DP family winged-helix DNA-binding domain [more]
AT4G19003.13.2e-9085.47E2F/DP family winged-helix DNA-binding domain [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014041ESCRT-II complex, Vps25 subunit, N-terminal winged helixGENE3D1.10.10.570coord: 5..101
e-value: 2.1E-31
score: 110.2
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 103..178
e-value: 2.2E-25
score: 90.1
IPR008570ESCRT-II complex, Vps25 subunitPFAMPF05871ESCRT-IIcoord: 13..145
e-value: 5.5E-43
score: 146.6
IPR008570ESCRT-II complex, Vps25 subunitPANTHERPTHR13149VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25coord: 1..179
NoneNo IPR availablePANTHERPTHR13149:SF1SUBFAMILY NOT NAMEDcoord: 1..179
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 102..177
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 7..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G01250.1CSPI03G01250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071985 multivesicular body sorting pathway
biological_process GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component GO:0000814 ESCRT II complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0005198 structural molecule activity