CSPI03G00800 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G00800
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	beta-carotene isomerase D27, chloroplastic
Location	Chr3: 536995 .. 540242 (-)
RNA-Seq Expression	CSPI03G00800
Synteny	CSPI03G00800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCATCATAAGATATGATATTAGATAAAGTGAAATAATAGGAAGGAAGAAGAAGCATATACTAACCATACCAAAGAGATTTTGTTGGAAGGAAAGAAAGAAAGTAGTTTTTTTGTTTTCCTAAAGGAAGTGCTTTTGATGGTGGTTTTGAAGCTACAAAGCATCCAATTCTTCACAGCTCCTCCAAAGGAGATTAGGAATAGAAAGATAAAGAGCAGATTTATTAGATGTGGGATAGCAGAGGCATCAGGTGAGCCAGCTCCATTGGGGCAGAAAACAAAGTACAATGATGGACCGTTCGAGAAGGTTTTTATGACACTGTTTGCAAAGAAAATGGAGAAATTTGCAAATGCTAAAGAGCAAAGGAAGAAGAAAGAAGGGTTATGGTGGGATTTTCTTTATGATTATGAAAGGTTTGTTGATGTTTCAAAGAGAGTAATGCAAGGGAAAAACCGAATGCAACAGCAGATCGTCGTTCGTGAGGTTCTCTTGTCTATGCTCCCTCCCGGCGCCCCGGCTCAGGTCATACCTTTTCTCTTATCTTTTCTCTTTTAGCTTCATTCGAACAAATTATTCAATGGGGTTGTTTTCACGTAACAGAATATATGAAGTTAAAATGTTGAACATCATACTGTGCTGTGGTTCATTGGAAAAATAATTTGAATTTCCATTTTGAAGAATGGTTGTGTAGGTGCATTTAAATGAATTCTACATAAAACTTTTACCATTTTCAATACCCGACGGTAAATAAGGTCAAATCGCATTGAAAAGTCTGGAAAGGTTCAAATTTCACGACCCTTGTGGATATAAAGAATTGAATCATAGACTTCTTGATCGATAGCATATTTTTAGTGCAGCTGAGTTATGCTTGTCTTTTAGCTCGTTTTGACCCATAACCCTTCTTTTATTTGCAATCAAAGGACATAGAAAAACCATATTTAGGGATTATATTTTCATCTTCTTCGATATATCATTGTGGACTAAATCAGAAGCTGATGCTGGCAGTTCAGGAAATTGTTCCCACCAACGAAATGGGCTTGTGAATTCAATGCTTTAATAACAGTTCCATTCTTTCAGTGGTTAGTCGGCCCTTCCGAGGTATTTTCATTTCATTAAATCTTCAAAATGCCCTCATCTTTATGAATCTCTTTGTATATTCTGTTTCACTTAGTACTCTCTGTTTTCTGTAGAATCGGTTTTGCACATACATGCTTTGAATAGCTATTGAACAAGTAATGTTAGTGAATGATTTGAAATTGCTTGCGTTCTATATCGTAGATGGCTTTTGTTTAGACCAAGGGTATAGGTATAATTAAATTTACGGTTCTTCGTCGACTTAAGGTTTTCAAACTTAGTCTCGTTTAGTAACTGTTTCATCTTTTGTTTTTAGTTTGAGTAGATTAAGCTCGTTTTCTCTCCATTCTTACTATGATTTGCATTCTTTCTTTTAAGTACAAAGGCTCTAGCAAAAACAAAAACAACATTTCAAAAAGCTACCTTTTTTAGTTTTCAAATTTTAGTTTAGCTTCTAAATCATTGGTAAAATGTAGAAAACAAAGGAAGAACGTTAATGGTTACCAAAACGGAGTCTTATTTATTTAAATTTTAAATACCTTTCAATATAAACTCTTTGTCGTGGTTAGTTTCTTTCTGCCCTTGATGTTATGTTATGTTAAGTTTGTTTATATCTTTCTCAAACGAAGTTTACTTGTGATCCCCTGCTGTGAGCTAAAATGCAGAATCCCAAAACAAGTTCCATTTTAGGATTTTAATTAGGAACGCAGTTTACAAAGCCTTCAACATAAGCTTGTAATTTGGGAGAAGAGGTAACATACATGCAAAACAAAGAAAACTGTCTCTAATGCCAAAGTTGGAGAGGTAGAAAACTTCAGAATTTAAATGAAGCTTGAACTTAAAGTTACCTCATATACTTATAAAGAGAATCGCCTAGTCTTAAATTAGTTAGGATTAGATCTAATTAGCCCTAGGCTTATTCAAGCCTAGCTAGGTCCAATTTCACCTACAATAACCATCAAATGATCTTTTGTTGGAGGGTGATCTGATTACTATTAGAGGCTGTGACAGCAGAAACCATGCATTGGCATTTTACTGAGTAATTTTGACATATAAACTTTCAATATGAATGAAAGAAACCTTAGATTTCCTCCTGTTTTAGGTTGTGGAAGTGGAGGTAAATGGTATAAAGCAAAGAAGTGGAGTTCATATAAAGAAATGCAGGTATAAAATCCATTTTCATTATGGCAGGTTTCACACAGCGATGTGCAAATTCTTCTATCAATCTACAATTACATAAGCATATATATATATTTGATTGTCTTTTTCTTTGCAATGTATTCCCATCTTAGGTACCTAGAGAACAGTGGATGTGTAGGTATGTGTGTGAATATGTGCAAGATACCTACACAAGATTTCTTCACTAATGAATTTGGACTCCCTCTCACCATGAATCCTAGTATGTATTCTGTGAATCTTTCTTTTCAATATCGTAATATGTTTTACAAGGATATCTAGATTTCTGGTTAGGTCAGAAGCCACTTTTGATCTTGAACGTACGTACTTTCATAGAAGTAACAATTTAATTCCTATACATTTGCTTTAGCTTTAGCATGTAACAATTTGATTTAAATATTTAAATAGTTGTAAAATTAAAGTACAATGACTTAATCGGGTCAAAAGTATTTTTTTTAACTTTATTAGCTATGTTTGGAGCGAAGATATTAGATTTAAATTGTTCTCGTCGTTAGATTTTGAAGACATGAGCTGTGAGATGATATATGGGCAAGTTCCACCACCATTTGAAGAGGATCCAGTTTCCAAACAACCTTGCTACAAAGATATATGTAAGAGGAATTTGTTTTGCTTTGCATTATTTCAATGATCAACCATATTTGATATAGAAAAAAAAATAATTAAGAAGTGCTCTTGGTTTTGATCAACTTATTAATGTGTCTTCTTGTGTGGTTGACAGGTTCTATGGCAATTACTAGTGCTCCCTTATGTCCTAAATTGCTAGCTTAAATACATTGGGGTACTTCTAGTAATACACTCATACAGTCATACGTTGATCGTATATATTATCTTGCTAGTCGGGAGGCAATAACGTTGTTATTTCTTTGCAAACGGTATTGTGTTTGCTAGATAATATACAATCAAGAAGATAATCTTCTTAACATGTTTATAAGAATATAGGGAAATTTTTCATGGTGTATTATTTTTATTTTGATGAGAG

mRNA sequence

GCATCATAAGATATGATATTAGATAAAGTGAAATAATAGGAAGGAAGAAGAAGCATATACTAACCATACCAAAGAGATTTTGTTGGAAGGAAAGAAAGAAAGTAGTTTTTTTGTTTTCCTAAAGGAAGTGCTTTTGATGGTGGTTTTGAAGCTACAAAGCATCCAATTCTTCACAGCTCCTCCAAAGGAGATTAGGAATAGAAAGATAAAGAGCAGATTTATTAGATGTGGGATAGCAGAGGCATCAGGTGAGCCAGCTCCATTGGGGCAGAAAACAAAGTACAATGATGGACCGTTCGAGAAGGTTTTTATGACACTGTTTGCAAAGAAAATGGAGAAATTTGCAAATGCTAAAGAGCAAAGGAAGAAGAAAGAAGGGTTATGGTGGGATTTTCTTTATGATTATGAAAGGTTTGTTGATGTTTCAAAGAGAGTAATGCAAGGGAAAAACCGAATGCAACAGCAGATCGTCGTTCGTGAGGTTCTCTTGTCTATGCTCCCTCCCGGCGCCCCGGCTCAGTTCAGGAAATTGTTCCCACCAACGAAATGGGCTTGTGAATTCAATGCTTTAATAACAGTTCCATTCTTTCAGTGGTTAGTCGGCCCTTCCGAGGTTGTGGAAGTGGAGGTAAATGGTATAAAGCAAAGAAGTGGAGTTCATATAAAGAAATGCAGGTACCTAGAGAACAGTGGATGTGTAGGTATGTGTGTGAATATGTGCAAGATACCTACACAAGATTTCTTCACTAATGAATTTGGACTCCCTCTCACCATGAATCCTAATTTTGAAGACATGAGCTGTGAGATGATATATGGGCAAGTTCCACCACCATTTGAAGAGGATCCAGTTTCCAAACAACCTTGCTACAAAGATATATGTTCTATGGCAATTACTAGTGCTCCCTTATGTCCTAAATTGCTAGCTTAAATACATTGGGGTACTTCTAGTAATACACTCATACAGTCATACGTTGATCGTATATATTATCTTGCTAGTCGGGAGGCAATAACGTTGTTATTTCTTTGCAAACGGTATTGTGTTTGCTAGATAATATACAATCAAGAAGATAATCTTCTTAACATGTTTATAAGAATATAGGGAAATTTTTCATGGTGTATTATTTTTATTTTGATGAGAG

Coding sequence (CDS)

ATGGTGGTTTTGAAGCTACAAAGCATCCAATTCTTCACAGCTCCTCCAAAGGAGATTAGGAATAGAAAGATAAAGAGCAGATTTATTAGATGTGGGATAGCAGAGGCATCAGGTGAGCCAGCTCCATTGGGGCAGAAAACAAAGTACAATGATGGACCGTTCGAGAAGGTTTTTATGACACTGTTTGCAAAGAAAATGGAGAAATTTGCAAATGCTAAAGAGCAAAGGAAGAAGAAAGAAGGGTTATGGTGGGATTTTCTTTATGATTATGAAAGGTTTGTTGATGTTTCAAAGAGAGTAATGCAAGGGAAAAACCGAATGCAACAGCAGATCGTCGTTCGTGAGGTTCTCTTGTCTATGCTCCCTCCCGGCGCCCCGGCTCAGTTCAGGAAATTGTTCCCACCAACGAAATGGGCTTGTGAATTCAATGCTTTAATAACAGTTCCATTCTTTCAGTGGTTAGTCGGCCCTTCCGAGGTTGTGGAAGTGGAGGTAAATGGTATAAAGCAAAGAAGTGGAGTTCATATAAAGAAATGCAGGTACCTAGAGAACAGTGGATGTGTAGGTATGTGTGTGAATATGTGCAAGATACCTACACAAGATTTCTTCACTAATGAATTTGGACTCCCTCTCACCATGAATCCTAATTTTGAAGACATGAGCTGTGAGATGATATATGGGCAAGTTCCACCACCATTTGAAGAGGATCCAGTTTCCAAACAACCTTGCTACAAAGATATATGTTCTATGGCAATTACTAGTGCTCCCTTATGTCCTAAATTGCTAGCTTAA

Protein sequence

MVVLKLQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLSMLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSKQPCYKDICSMAITSAPLCPKLLA*

Homology

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BLAST of CSPI03G00800 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 5.2e-41
Identity = 83/210 (39.52%), Postives = 122/210 (58.10%), Query Frame = 0


Query: 49  YNDGPFEKVFMTLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQ 108
           Y D  F+K+ +   +K ++        +             ++  V+ +    Q  N  Q
Sbjct: 53  YKDNWFDKLAINHLSKSVQAATGISNNKS-----------GFDSLVEAATVASQKFNTTQ 112

Query: 109 QQIVVREVLLSMLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGI 168
           QQ ++ + L    P    +  R++ PP+K A E+ A+ T  FF WL+GPSEV E E+NG 
Sbjct: 113 QQGIILDALDRAFPKPILSVIRRVMPPSKLAREYFAVFTTIFFAWLLGPSEVRESEINGR 172

Query: 169 KQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQ 228
           ++++ VHIKKCR+LE + CVGMC+N+CK+P+Q F  + FG+P+ M PNF+DMSCEMI+GQ
Sbjct: 173 REKNIVHIKKCRFLEETNCVGMCINLCKMPSQLFIKDSFGMPVNMVPNFDDMSCEMIFGQ 232

Query: 229 VPPPFEEDPVSKQPCYKDICSMAITSAPLC 259
            PP   +DP  KQPCYK +C      A  C
Sbjct: 233 EPPASTDDPALKQPCYK-LCKAKKNHATQC 250

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BLAST of CSPI03G00800 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 7.5e-40
Identity = 88/224 (39.29%), Postives = 125/224 (55.80%), Query Frame = 0


Query: 26  SRFIRCGIAEASGEPAPLGQKTKYN----DGPFEKVFMTLFAKKMEKFANAKEQRKKKEG 85
           S+ +  G  +A+ E A +      N    D  F K+ +   +K ++  A      K    
Sbjct: 38  SKPVYSGKLKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKST-- 97

Query: 86  LWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLSMLPPGAPAQFRKLFPPTKWACE 145
                  DY+R VD + RV +  +  QQ   V   L   LP    +  +  FPP+K + E
Sbjct: 98  -------DYDRLVDTATRVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRE 157

Query: 146 FNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQD 205
             AL T   F WLVGPSEV E EVNG K++S V+I+KCR+LE S CVGMC ++CKIP+Q 
Sbjct: 158 LFALFTTISFAWLVGPSEVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQI 217

Query: 206 FFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSKQPCYK 246
           F  N  G+P+ M P+F D+SC+M++G+ PP  E+DP  KQPC++
Sbjct: 218 FIKNSLGMPIYMEPDFNDLSCKMMFGREPPEIEDDPAMKQPCFE 252

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BLAST of CSPI03G00800 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.4e-38
Identity = 81/210 (38.57%), Postives = 124/210 (59.05%), Query Frame = 0


Query: 39  EPAPLGQKTKYNDGPFEKVFMTLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSK 98
           E A     T Y D  F+K+ +   ++ +++ +  K +   K+G        YE  +D + 
Sbjct: 70  ETAAAPPATVYRDSWFDKLAIGYLSRNLQEASGLKNE---KDG--------YESLIDAAL 129

Query: 99  RVMQGKNRMQQQIVVREVLLSMLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPS 158
            + +  +  +Q  +V + L   LP       + + PP++++ E+ A  T  FF WLVGP 
Sbjct: 130 AISRIFSLDKQSEIVTQALERALPSYILTMIKVMMPPSRFSREYFAAFTTIFFPWLVGPC 189

Query: 159 EVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFE 218
           EV+E EV G K+++ V+I KCR+LE++ CVGMC N+CKIP Q F  +  G+ + M+PNFE
Sbjct: 190 EVMESEVEGRKEKNVVYIPKCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVYMSPNFE 249

Query: 219 DMSCEMIYGQVPPPFEEDPVSKQPCYKDIC 249
           DMSCEMI+GQ PP  E+DP  KQPC++  C
Sbjct: 250 DMSCEMIFGQQPP--EDDPALKQPCFRTKC 266

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BLAST of CSPI03G00800 vs. ExPASy TrEMBL
Match: A0A0A0L682 (DUF4033 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002850 PE=4 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 5.8e-152
Identity = 261/263 (99.24%), Postives = 262/263 (99.62%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 60
           MVVLKLQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT
Sbjct: 1   MVVLKLQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 60

Query: 61  LFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLSM 120
           LFA+KMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGK RMQQQIVVREVLLSM
Sbjct: 61  LFARKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKTRMQQQIVVREVLLSM 120

Query: 121 LPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR 180
           LPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR
Sbjct: 121 LPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR 180

Query: 181 YLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSK 240
           YLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSK
Sbjct: 181 YLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSK 240

Query: 241 QPCYKDICSMAITSAPLCPKLLA 264
           QPCYKDICSMAITSAPLCPKLLA
Sbjct: 241 QPCYKDICSMAITSAPLCPKLLA 263

[Close]

BLAST of CSPI03G00800 vs. ExPASy TrEMBL
Match: A0A5A7UFH7 (Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00760 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 3.6e-146
Identity = 256/264 (96.97%), Postives = 260/264 (98.48%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKI-KSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFM 60
           MVVLKLQSIQFFTAPPKEIRNRKI KSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFM
Sbjct: 1   MVVLKLQSIQFFTAPPKEIRNRKISKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFM 60

Query: 61  TLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLS 120
           TLFA+KMEKFANAKEQRKKKEG +WDFLYDYERFVDVSKRVMQGKNRMQQQ+VVREVLLS
Sbjct: 61  TLFARKMEKFANAKEQRKKKEG-FWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREVLLS 120

Query: 121 MLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKC 180
           MLPPGAPAQFRKLFPPTKWACEFNA ITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKC
Sbjct: 121 MLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKC 180

Query: 181 RYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS 240
           RYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS
Sbjct: 181 RYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS 240

Query: 241 KQPCYKDICSMAITSAPLCPKLLA 264
           KQPCYKDICSM+ TSAPLCPKLLA
Sbjct: 241 KQPCYKDICSMSNTSAPLCPKLLA 263

[Close]

BLAST of CSPI03G00800 vs. ExPASy TrEMBL
Match: A0A1S3BKU4 (beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103490700 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 3.6e-146
Identity = 256/264 (96.97%), Postives = 260/264 (98.48%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKI-KSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFM 60
           MVVLKLQSIQFFTAPPKEIRNRKI KSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFM
Sbjct: 1   MVVLKLQSIQFFTAPPKEIRNRKISKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFM 60

Query: 61  TLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLS 120
           TLFA+KMEKFANAKEQRKKKEG +WDFLYDYERFVDVSKRVMQGKNRMQQQ+VVREVLLS
Sbjct: 61  TLFARKMEKFANAKEQRKKKEG-FWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREVLLS 120

Query: 121 MLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKC 180
           MLPPGAPAQFRKLFPPTKWACEFNA ITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKC
Sbjct: 121 MLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKC 180

Query: 181 RYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS 240
           RYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS
Sbjct: 181 RYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS 240

Query: 241 KQPCYKDICSMAITSAPLCPKLLA 264
           KQPCYKDICSM+ TSAPLCPKLLA
Sbjct: 241 KQPCYKDICSMSNTSAPLCPKLLA 263

[Close]

BLAST of CSPI03G00800 vs. ExPASy TrEMBL
Match: A0A6J1FRN3 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111447551 PE=4 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.1e-133
Identity = 239/268 (89.18%), Postives = 247/268 (92.16%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKIK-----SRFIRCGIAEASGEPAPLGQKTKYNDGPFE 60
           MVVLKLQS QFFTA P EIR+RK       SR IRCGIAEASGEPAPLGQKTKYNDGPFE
Sbjct: 1   MVVLKLQSAQFFTA-PCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFE 60

Query: 61  KVFMTLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVRE 120
           KVFMTLFAKKME++AN+KEQ KK    WWDFLYDYERFVD SKRVMQGKNRMQQQ+VVRE
Sbjct: 61  KVFMTLFAKKMERYANSKEQMKKIG--WWDFLYDYERFVDASKRVMQGKNRMQQQLVVRE 120

Query: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVH 180
           VLLSMLPPGAPAQFRKLFPPTKWACEFNA ITVPFFQWLVGPSEVVEVEVNG+KQRSGVH
Sbjct: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGMKQRSGVH 180

Query: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 240
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE
Sbjct: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 240

Query: 241 DPVSKQPCYKDICSMAITSAPLCPKLLA 264
           DPVSKQPCYKDICSM+  S+ LCPKLLA
Sbjct: 241 DPVSKQPCYKDICSMSNPSSSLCPKLLA 265

[Close]

BLAST of CSPI03G00800 vs. ExPASy TrEMBL
Match: A0A6J1L2P3 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111498565 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.3e-132
Identity = 239/268 (89.18%), Postives = 246/268 (91.79%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKIK-----SRFIRCGIAEASGEPAPLGQKTKYNDGPFE 60
           MVVLKLQS QFFTA P EIR+RK       SR IRCGIAEASGEPAPLGQKTKYNDGPFE
Sbjct: 1   MVVLKLQSAQFFTA-PCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFE 60

Query: 61  KVFMTLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVRE 120
           KVFMTLFAKKME++AN+KEQ KK    WWDFLYDYERFVD SKRVMQGKNRMQQQ+VVRE
Sbjct: 61  KVFMTLFAKKMERYANSKEQTKKIG--WWDFLYDYERFVDASKRVMQGKNRMQQQLVVRE 120

Query: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVH 180
           VLLSMLPPGAPAQFRKLFPPTKWACEFNA ITVPFFQWLVGPSEVVEVEVNGIKQRSGVH
Sbjct: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVH 180

Query: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 240
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP FEE
Sbjct: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPLFEE 240

Query: 241 DPVSKQPCYKDICSMAITSAPLCPKLLA 264
           DPVSKQPCYKDICSM+  S+ LCPKLLA
Sbjct: 241 DPVSKQPCYKDICSMSNPSSSLCPKLLA 265

[Close]

BLAST of CSPI03G00800 vs. NCBI nr
Match: XP_011650294.1 (beta-carotene isomerase D27, chloroplastic [Cucumis sativus] >KGN55666.1 hypothetical protein Csa_010048 [Cucumis sativus])

HSP 1 Score: 546.6 bits (1407), Expect = 1.2e-151
Identity = 261/263 (99.24%), Postives = 262/263 (99.62%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 60
           MVVLKLQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT
Sbjct: 1   MVVLKLQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 60

Query: 61  LFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLSM 120
           LFA+KMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGK RMQQQIVVREVLLSM
Sbjct: 61  LFARKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKTRMQQQIVVREVLLSM 120

Query: 121 LPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR 180
           LPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR
Sbjct: 121 LPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR 180

Query: 181 YLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSK 240
           YLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSK
Sbjct: 181 YLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSK 240

Query: 241 QPCYKDICSMAITSAPLCPKLLA 264
           QPCYKDICSMAITSAPLCPKLLA
Sbjct: 241 QPCYKDICSMAITSAPLCPKLLA 263

[Close]

BLAST of CSPI03G00800 vs. NCBI nr
Match: XP_008448557.1 (PREDICTED: beta-carotene isomerase D27, chloroplastic [Cucumis melo] >KAA0052966.1 beta-carotene isomerase D27 [Cucumis melo var. makuwa] >TYK11422.1 beta-carotene isomerase D27 [Cucumis melo var. makuwa])

HSP 1 Score: 527.3 bits (1357), Expect = 7.5e-146
Identity = 256/264 (96.97%), Postives = 260/264 (98.48%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKI-KSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFM 60
           MVVLKLQSIQFFTAPPKEIRNRKI KSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFM
Sbjct: 1   MVVLKLQSIQFFTAPPKEIRNRKISKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFM 60

Query: 61  TLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLS 120
           TLFA+KMEKFANAKEQRKKKEG +WDFLYDYERFVDVSKRVMQGKNRMQQQ+VVREVLLS
Sbjct: 61  TLFARKMEKFANAKEQRKKKEG-FWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREVLLS 120

Query: 121 MLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKC 180
           MLPPGAPAQFRKLFPPTKWACEFNA ITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKC
Sbjct: 121 MLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKC 180

Query: 181 RYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS 240
           RYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS
Sbjct: 181 RYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS 240

Query: 241 KQPCYKDICSMAITSAPLCPKLLA 264
           KQPCYKDICSM+ TSAPLCPKLLA
Sbjct: 241 KQPCYKDICSMSNTSAPLCPKLLA 263

[Close]

BLAST of CSPI03G00800 vs. NCBI nr
Match: XP_038876564.1 (beta-carotene isomerase D27, chloroplastic [Benincasa hispida])

HSP 1 Score: 509.2 bits (1310), Expect = 2.1e-140
Identity = 244/261 (93.49%), Postives = 251/261 (96.17%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 60
           MVV+KLQSIQFFTAPPKEIR RK +SRF+RCGIAEASGEPAPLGQKTKYNDGPFEKVFMT
Sbjct: 1   MVVVKLQSIQFFTAPPKEIRIRKKQSRFVRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 60

Query: 61  LFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLSM 120
           LFA+KMEKFAN KEQ KKK GL WDFLYDYERFVDVSKRVMQGKNRMQQQ+VVREVLLSM
Sbjct: 61  LFARKMEKFANTKEQGKKKVGL-WDFLYDYERFVDVSKRVMQGKNRMQQQLVVREVLLSM 120

Query: 121 LPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR 180
           LPPGAPAQFRKLFPPTKWACEFNA ITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR
Sbjct: 121 LPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR 180

Query: 181 YLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSK 240
           YLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVS 
Sbjct: 181 YLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSN 240

Query: 241 QPCYKDICSMAITSAPLCPKL 262
           QPCYKDICSMA +S P+CPKL
Sbjct: 241 QPCYKDICSMANSSTPVCPKL 260

[Close]

BLAST of CSPI03G00800 vs. NCBI nr
Match: XP_023540699.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 487.3 bits (1253), Expect = 8.6e-134
Identity = 241/268 (89.93%), Postives = 247/268 (92.16%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKIK-----SRFIRCGIAEASGEPAPLGQKTKYNDGPFE 60
           MVVLKLQS QFFTA P EIR+RK       SR IRCGIAEASGEPAPLGQKTKYNDGPFE
Sbjct: 1   MVVLKLQSAQFFTA-PCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFE 60

Query: 61  KVFMTLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVRE 120
           KVFMTLFAKKMEK+AN+KEQ KK    WWDFLYDYERFVD SKRVMQGKNRMQQQ+VVRE
Sbjct: 61  KVFMTLFAKKMEKYANSKEQTKKIG--WWDFLYDYERFVDASKRVMQGKNRMQQQLVVRE 120

Query: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVH 180
           VLLSMLPPGAPAQFRKLFPPTKWACEFNA ITVPFFQWLVGPSEVVEVEVNGIKQRSGVH
Sbjct: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVH 180

Query: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 240
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE
Sbjct: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 240

Query: 241 DPVSKQPCYKDICSMAITSAPLCPKLLA 264
           DPVSKQPCYKDICSM+  S+ LCPKLLA
Sbjct: 241 DPVSKQPCYKDICSMSNPSSSLCPKLLA 265

[Close]

BLAST of CSPI03G00800 vs. NCBI nr
Match: XP_022942554.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita moschata])

HSP 1 Score: 485.0 bits (1247), Expect = 4.2e-133
Identity = 239/268 (89.18%), Postives = 247/268 (92.16%), Query Frame = 0


Query: 1   MVVLKLQSIQFFTAPPKEIRNRKIK-----SRFIRCGIAEASGEPAPLGQKTKYNDGPFE 60
           MVVLKLQS QFFTA P EIR+RK       SR IRCGIAEASGEPAPLGQKTKYNDGPFE
Sbjct: 1   MVVLKLQSAQFFTA-PCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFE 60

Query: 61  KVFMTLFAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVRE 120
           KVFMTLFAKKME++AN+KEQ KK    WWDFLYDYERFVD SKRVMQGKNRMQQQ+VVRE
Sbjct: 61  KVFMTLFAKKMERYANSKEQMKKIG--WWDFLYDYERFVDASKRVMQGKNRMQQQLVVRE 120

Query: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVH 180
           VLLSMLPPGAPAQFRKLFPPTKWACEFNA ITVPFFQWLVGPSEVVEVEVNG+KQRSGVH
Sbjct: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGMKQRSGVH 180

Query: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 240
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE
Sbjct: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 240

Query: 241 DPVSKQPCYKDICSMAITSAPLCPKLLA 264
           DPVSKQPCYKDICSM+  S+ LCPKLLA
Sbjct: 241 DPVSKQPCYKDICSMSNPSSSLCPKLLA 265

[Close]

BLAST of CSPI03G00800 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 386.0 bits (990), Expect = 2.5e-107
Identity = 181/243 (74.49%), Postives = 205/243 (84.36%), Query Frame = 0


Query: 21  NRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAKKMEKFANAKEQRKKKE 80
           N   + R  RCGIAE SGEPAP+G KT+Y DG  E+VFM LFA+KM+KF + K++  K++
Sbjct: 11  NLTFRRRSTRCGIAEPSGEPAPMGLKTRYEDGLVERVFMGLFARKMDKFGSKKKKDTKEK 70

Query: 81  GLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLSMLPPGAPAQFRKLFPPTKWAC 140
           G W    YDYE FV+VSKRVMQG++R+QQQ  VREVLLSMLPPGAP QFRKLFPPTKWA 
Sbjct: 71  GFW---EYDYESFVEVSKRVMQGRSRVQQQEAVREVLLSMLPPGAPEQFRKLFPPTKWAA 130

Query: 141 EFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQ 200
           EFNA +TVPFF WLVGPS+V+EVEVNG+KQRSGV IKKCRYLENSGCVGMCVNMCKIPTQ
Sbjct: 131 EFNAALTVPFFHWLVGPSQVIEVEVNGVKQRSGVRIKKCRYLENSGCVGMCVNMCKIPTQ 190

Query: 201 DFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSKQPCYKDICSMAITSAPLCPK 260
           DFFTNEFGLPLTMNPN+EDMSCEMIYGQ PP FEED  +KQPC  DICSM+  S+P+CPK
Sbjct: 191 DFFTNEFGLPLTMNPNYEDMSCEMIYGQAPPAFEEDVATKQPCLADICSMSNPSSPICPK 250

Query: 261 LLA 264
           L A
Sbjct: 251 LEA 250

[Close]

BLAST of CSPI03G00800 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 165.6 bits (418), Expect = 5.3e-41
Identity = 88/224 (39.29%), Postives = 125/224 (55.80%), Query Frame = 0


Query: 26  SRFIRCGIAEASGEPAPLGQKTKYN----DGPFEKVFMTLFAKKMEKFANAKEQRKKKEG 85
           S+ +  G  +A+ E A +      N    D  F K+ +   +K ++  A      K    
Sbjct: 38  SKPVYSGKLKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKST-- 97

Query: 86  LWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLSMLPPGAPAQFRKLFPPTKWACE 145
                  DY+R VD + RV +  +  QQ   V   L   LP    +  +  FPP+K + E
Sbjct: 98  -------DYDRLVDTATRVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRE 157

Query: 146 FNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQD 205
             AL T   F WLVGPSEV E EVNG K++S V+I+KCR+LE S CVGMC ++CKIP+Q 
Sbjct: 158 LFALFTTISFAWLVGPSEVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQI 217

Query: 206 FFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSKQPCYK 246
           F  N  G+P+ M P+F D+SC+M++G+ PP  E+DP  KQPC++
Sbjct: 218 FIKNSLGMPIYMEPDFNDLSCKMMFGREPPEIEDDPAMKQPCFE 252

[Close]

BLAST of CSPI03G00800 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 136.7 bits (343), Expect = 2.6e-32
Identity = 86/254 (33.86%), Postives = 130/254 (51.18%), Query Frame = 0


Query: 9   IQFFTAPPKEI------RNRKIKSRFIRCGIAEASGEPAPLG-QKTKYNDGPFEKVFMTL 68
           IQ    PP  I        + I +R +RC I+ +S   +  G  K +Y  GP +  FM  
Sbjct: 5   IQVLLPPPTTIVFSNSFSTKLILTRRLRCRISNSSEVKSDEGAPKLEYKPGPLDDFFMQS 64

Query: 69  FAKKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLSML 128
           F  K+ +   +  ++    GL             V   +++G+ R +       +L S+ 
Sbjct: 65  FRNKLVEEVGSDSEKPGYVGL----------IELVKLLLLKGRTRSETSDAAVRILKSLF 124

Query: 129 PPGAPAQFRKLFPPT---KWACEFNALITVPFFQWLVGPSEVVEVEV-NGIKQRSGVHIK 188
           PP     ++ L  P    K A    A +TV   QWL+GPS+V  +++ NG    SGV ++
Sbjct: 125 PPLILELYKLLIAPIAQGKLAALMVARVTVLTCQWLMGPSKVNIIDLPNGESWDSGVFVE 184

Query: 189 KCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDP 248
           KC+YLE S CVG+C+N CK+PTQ FF +  G+PL M PNF+D SC+  +G  PP  E+D 
Sbjct: 185 KCQYLEESKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKDYSCQFKFGVAPP--EDDG 244

Query: 249 VSKQPCYKDICSMA 252
              +PC+ + CS+A
Sbjct: 245 NVNEPCF-ETCSIA 245

[Close]

BLAST of CSPI03G00800 vs. TAIR 10
Match: AT1G03055.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 79.0 bits (193), Expect = 6.5e-15
Identity = 54/159 (33.96%), Postives = 76/159 (47.80%), Query Frame = 0


Query: 26  SRFIRCGIAEASGEPAPLGQKTKYN----DGPFEKVFMTLFAKKMEKFANAKEQRKKKEG 85
           S+ +  G  +A+ E A +      N    D  F K+ +   +K ++  A      K    
Sbjct: 38  SKPVYSGKLKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKST-- 97

Query: 86  LWWDFLYDYERFVDVSKRVMQGKNRMQQQIVVREVLLSMLPPGAPAQFRKLFPPTKWACE 145
                  DY+R VD + RV +  +  QQ   V   L   LP    +  +  FPP+K + E
Sbjct: 98  -------DYDRLVDTATRVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRE 157

Query: 146 FNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR 181
             AL T   F WLVGPSEV E EVNG K++S V+I+KCR
Sbjct: 158 LFALFTTISFAWLVGPSEVRETEVNGRKEKSVVYIEKCR 187

The following BLAST results are available for this feature:

BLAST of CSPI03G00800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
R4HZ96	5.2e-41	39.52	Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27...	[more]
Q7XA78	7.5e-40	39.29	Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D2...	[more]
C7AU21	2.4e-38	38.57	Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39...	[more]

BLAST of CSPI03G00800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L682	5.8e-152	99.24	DUF4033 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002850 PE=...	[more]
A0A5A7UFH7	3.6e-146	96.97	Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BKU4	3.6e-146	96.97	beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034907...	[more]
A0A6J1FRN3	2.1e-133	89.18	beta-carotene isomerase D27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1L2P3	1.3e-132	89.18	beta-carotene isomerase D27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of CSPI03G00800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650294.1	1.2e-151	99.24	beta-carotene isomerase D27, chloroplastic [Cucumis sativus] >KGN55666.1 hypothe...	[more]
XP_008448557.1	7.5e-146	96.97	PREDICTED: beta-carotene isomerase D27, chloroplastic [Cucumis melo] >KAA0052966...	[more]
XP_038876564.1	2.1e-140	93.49	beta-carotene isomerase D27, chloroplastic [Benincasa hispida]	[more]
XP_023540699.1	8.6e-134	89.93	beta-carotene isomerase D27, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022942554.1	4.2e-133	89.18	beta-carotene isomerase D27, chloroplastic [Cucurbita moschata]	[more]

BLAST of CSPI03G00800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64680.1	2.5e-107	74.49	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G03055.1	5.3e-41	39.29	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT4G01995.1	2.6e-32	33.86	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G03055.2	6.5e-15	33.96	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025114	Beta-carotene isomerase D27-like, C-terminal	PFAM	PF13225	DUF4033	coord: 153..235 e-value: 1.1E-38 score: 131.1
None	No IPR available	PANTHER	PTHR33591:SF4	OS08G0114100 PROTEIN	coord: 18..261
IPR038938	Beta-carotene isomerase D27-like	PANTHER	PTHR33591	BETA-CAROTENE ISOMERASE D27	coord: 18..261

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G00800.1	CSPI03G00800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016853	isomerase activity