CSPI03G00500 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G00500
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Aconitate hydratase
Location	Chr3: 370511 .. 371282 (-)
RNA-Seq Expression	CSPI03G00500
Synteny	CSPI03G00500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAGCTTTGCTAGACCAAAGGAGTCACAGACTAAGGTTGTAGAGTTCAGTTTTCATGGAACAACAGCTGAGCTTAGGCATGGTGATGTTGTAATAGCAGCGATTGCTAGCTGCACCAATACCTCCAATCCTAGTATAAGGCTTGGGGCTGCTTTGGTTGCCAAGAAAGCTTGTGAACTTAGTTTAGAGGTGTGAAAGTTCTACCTCCTCACATTGCCTGTGCATCAATAATTTATTTCAGAAGGACAGTGACGAACAAAATGAAACATTTTATAAATTATATTCCCAAACACACCATTAATATTTGGCTTTCTAAAGAGGTTAAGCCATGGATAAACACCGGTCTTGCTCCAGGTGCAAGAGTGGTAACCAAATACTTGGAAAAGAGGTGCCACTATCAAACTCTCCAAATGCTTCTGTTGTTAAATGAGAAATAAAAATTGAATAAAAACACTCAACAACGAATGAACTAATATAGTTCCATTATTCTTCTGACTGGGAGTTTTTCTGTTTCTCTTCTTGTTGTTGCTGATGTTGTGTGGAGGGTAATATTTCTCAAGTCAAGAGTTGGATTTCTGCAGTGAATTGTAGAAGTATTTAGATAAGCTGGGGTTCAATACTGTCGGGGAGTATGGATGCACAACATACATTGGCAATTCAGGGGATTTAGATGAAGAAGTGGCATCTGCAATTTCTGACGACGAATAGAAAAGTTTAATTATTACCACTTGTACCCTCTAGCCTCTCTAGCTCAGGCAAGCAATTGAAT

mRNA sequence

ATGCAGAGCTTTGCTAGACCAAAGGAGTCACAGACTAAGGTTGTAGAGTTCAGTTTTCATGGAACAACAGCTGAGCTTAGGCATGGTGATGTTGTAATAGCAGCGATTGCTAGCTGCACCAATACCTCCAATCCTAGTATAAGGCTTGGGGCTGCTTTGGTTGCCAAGAAAGCTTGTGAACTTAGTTTAGAGGTTAAGCCATGGATAAACACCGGTCTTGCTCCAGGTGCAAGAGTGGTAACCAAATACTTGGAAAAGAGTTCCATTATTCTTCTGACTGGGAGTTTTTCTGTTTCTCTTCTTGTTGTTGCTGATGTTGTGTGGAGGAAGTATTTAGATAAGCTGGGGTTCAATACTGTCGGGGAGTATGGATGCACAACATACATTGGCAATTCAGGGGATTTAGATGAAGAAGTGGCATCTGCAATTTCTGACGACGAATAGAAAAGTTTAATTATTACCACTTGTACCCTCTAGCCTCTCTAGCTCAGGCAAGCAATTGAAT

Coding sequence (CDS)

ATGCAGAGCTTTGCTAGACCAAAGGAGTCACAGACTAAGGTTGTAGAGTTCAGTTTTCATGGAACAACAGCTGAGCTTAGGCATGGTGATGTTGTAATAGCAGCGATTGCTAGCTGCACCAATACCTCCAATCCTAGTATAAGGCTTGGGGCTGCTTTGGTTGCCAAGAAAGCTTGTGAACTTAGTTTAGAGGTTAAGCCATGGATAAACACCGGTCTTGCTCCAGGTGCAAGAGTGGTAACCAAATACTTGGAAAAGAGTTCCATTATTCTTCTGACTGGGAGTTTTTCTGTTTCTCTTCTTGTTGTTGCTGATGTTGTGTGGAGGAAGTATTTAGATAAGCTGGGGTTCAATACTGTCGGGGAGTATGGATGCACAACATACATTGGCAATTCAGGGGATTTAGATGAAGAAGTGGCATCTGCAATTTCTGACGACGAATAG

Protein sequence

MQSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACELSLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE*

Homology

[Close]

BLAST of CSPI03G00500 vs. ExPASy Swiss-Prot
Match: Q42560 (Aconitate hydratase 1 OS=Arabidopsis thaliana OX=3702 GN=ACO1 PE=1 SV=2)

HSP 1 Score: 175.6 bits (444), Expect = 4.1e-43
Identity = 93/146 (63.70%), Postives = 110/146 (75.34%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q+K VEF+F+GTTA+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKAC+L
Sbjct: 404 KGFAVPKEAQSKAVEFNFNGTTAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACDL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYL KS +                   +KYL++LGF+ VG
Sbjct: 464 GLEVKPWIKTSLAPGSGVVTKYLAKSGL-------------------QKYLNQLGFSIVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+ E VASAI D++
Sbjct: 524 -YGCTTCIGNSGDIHEAVASAIVDND 529

[Close]

BLAST of CSPI03G00500 vs. ExPASy Swiss-Prot
Match: Q6YZX6 (Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0191100 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 5.0e-41
Identity = 89/146 (60.96%), Postives = 106/146 (72.60%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE Q KVV+F FHG  AEL+HG VVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 404 KGFAVPKEQQDKVVKFDFHGQPAELKHGSVVIAAITSCTNTSNPSVMLGAALVAKKACEL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPW+ T LAPG+ VVTKYL +S +                   ++YL+K GF+ VG
Sbjct: 464 GLEVKPWVKTSLAPGSGVVTKYLLQSGL-------------------QEYLNKQGFHVVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGDLDE V++AIS+++
Sbjct: 524 -YGCTTCIGNSGDLDESVSAAISEND 529

[Close]

BLAST of CSPI03G00500 vs. ExPASy Swiss-Prot
Match: O04916 (Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.1e-40
Identity = 91/146 (62.33%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE Q KV +FSFHG  AEL+HG VVIAAI SCTNTSNPS+ LGAALVAKKA EL
Sbjct: 122 KGFAVPKEVQDKVAKFSFHGQPAELKHGSVVIAAITSCTNTSNPSVMLGAALVAKKASEL 181

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            L VKPW+ T LAPG+ VVTKYL KS +                   +KYL++ GFN VG
Sbjct: 182 GLHVKPWVKTSLAPGSGVVTKYLLKSGL-------------------QKYLNQQGFNIVG 241

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGDLDE VASAIS+++
Sbjct: 242 -YGCTTCIGNSGDLDESVASAISEND 247

[Close]

BLAST of CSPI03G00500 vs. ExPASy Swiss-Prot
Match: P49608 (Aconitate hydratase, cytoplasmic OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.5e-40
Identity = 88/146 (60.27%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q  V +FSFHG  AEL+HG VVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 404 KGFAIPKEAQENVAKFSFHGQPAELKHGSVVIAAITSCTNTSNPSVMLGAALVAKKACEL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            L+VKPW+ T LAPG+ VVTKYL KS +                   + YL++ GF+ VG
Sbjct: 464 GLQVKPWVKTSLAPGSGVVTKYLLKSGL-------------------QPYLNQQGFHIVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGDLDE V++AISD++
Sbjct: 524 -YGCTTCIGNSGDLDESVSAAISDND 529

[Close]

BLAST of CSPI03G00500 vs. ExPASy Swiss-Prot
Match: Q42669 (Aconitate hydratase (Fragment) OS=Cucumis melo var. conomon OX=3657 GN=ACO PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 1.8e-38
Identity = 89/146 (60.96%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q KV EF+FHG+ A+LRHGDVVIAAI SCTNTS+ S+ LGAALVAKKACEL
Sbjct: 270 KGFAIPKEAQVKVAEFNFHGSPAQLRHGDVVIAAITSCTNTSS-SVMLGAALVAKKACEL 329

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T L     VVTKYL KS +                   +KYL++LGFN VG
Sbjct: 330 GLEVKPWIKTVLLQALGVVTKYLAKSGL-------------------QKYLNQLGFNIVG 389

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+DE VASAI+ ++
Sbjct: 390 -YGCTTCIGNSGDIDESVASAITGND 394

[Close]

BLAST of CSPI03G00500 vs. ExPASy TrEMBL
Match: A0A0A0L3M2 (Aconitase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002540 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.1e-70
Identity = 143/156 (91.67%), Postives = 145/156 (92.95%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           +SFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKK CEL
Sbjct: 29  KSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKVCEL 88

Query: 62  SLE----------VKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKY 121
           SLE          VKPWINTGLAPG+RVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKY
Sbjct: 89  SLEVCKFYLLTLPVKPWINTGLAPGSRVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKY 148

Query: 122 LDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE 148
           LDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE
Sbjct: 149 LDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE 184

[Close]

BLAST of CSPI03G00500 vs. ExPASy TrEMBL
Match: A0A6J1CW20 (Aconitate hydratase OS=Momordica charantia OX=3673 GN=LOC111014921 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 6.3e-47
Identity = 106/146 (72.60%), Postives = 122/146 (83.56%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           +SFA PKESQTKVVEFSFHG  A LRHGDVVIAAI SCTNTSNPSI LGAALVAKKACEL
Sbjct: 188 KSFAIPKESQTKVVEFSFHGKPAALRHGDVVIAAITSCTNTSNPSIMLGAALVAKKACEL 247

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG++VVTKYL+K    L + +   +LL++ +   +KYLD+LGFNTV 
Sbjct: 248 GLEVKPWIKTSLAPGSKVVTKYLQKRCAPLDSPN---ALLMLDENGLQKYLDQLGFNTV- 307

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
           EYGCTT IGNSGDLDEEVASAI++++
Sbjct: 308 EYGCTTCIGNSGDLDEEVASAITEND 329

[Close]

BLAST of CSPI03G00500 vs. ExPASy TrEMBL
Match: A0A6J1CVD7 (Aconitate hydratase OS=Momordica charantia OX=3673 GN=LOC111014921 PE=3 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.8e-46
Identity = 104/146 (71.23%), Postives = 115/146 (78.77%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           +SFA PKESQTKVVEFSFHG  A LRHGDVVIAAI SCTNTSNPSI LGAALVAKKACEL
Sbjct: 188 KSFAIPKESQTKVVEFSFHGKPAALRHGDVVIAAITSCTNTSNPSIMLGAALVAKKACEL 247

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG++VVTKYL+KS +                   +KYLD+LGFNTV 
Sbjct: 248 GLEVKPWIKTSLAPGSKVVTKYLQKSGL-------------------QKYLDQLGFNTV- 307

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
           EYGCTT IGNSGDLDEEVASAI++++
Sbjct: 308 EYGCTTCIGNSGDLDEEVASAITEND 313

[Close]

BLAST of CSPI03G00500 vs. ExPASy TrEMBL
Match: A0A5A7UEJ6 (Aconitate hydratase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00450 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.4e-46
Identity = 107/145 (73.79%), Postives = 112/145 (77.24%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PK+SQTKVVEFSF G TAELRHGDVVIAAI SCTNTSNPSI LGAALVAKKACEL
Sbjct: 118 KGFAIPKKSQTKVVEFSFRGRTAELRHGDVVIAAITSCTNTSNPSIMLGAALVAKKACEL 177

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVK WI TGLAPG+ VVTKYLEKS +                   +KYLDKLGFNTVG
Sbjct: 178 CLEVKHWIKTGLAPGSIVVTKYLEKSGL-------------------QKYLDKLGFNTVG 237

Query: 122 EYGCTTYIGNSGDLDEEVASAISDD 147
            YGCTT  GNSGDLDEEVASAISDD
Sbjct: 238 -YGCTTCSGNSGDLDEEVASAISDD 242

[Close]

BLAST of CSPI03G00500 vs. ExPASy TrEMBL
Match: A0A5D3CJP4 (Aconitate hydratase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00470 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.4e-46
Identity = 107/145 (73.79%), Postives = 112/145 (77.24%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PK+SQTKVVEFSF G TAELRHGDVVIAAI SCTNTSNPSI LGAALVAKKACEL
Sbjct: 157 KGFAIPKKSQTKVVEFSFRGRTAELRHGDVVIAAITSCTNTSNPSIMLGAALVAKKACEL 216

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVK WI TGLAPG+ VVTKYLEKS +                   +KYLDKLGFNTVG
Sbjct: 217 CLEVKHWIKTGLAPGSIVVTKYLEKSGL-------------------QKYLDKLGFNTVG 276

Query: 122 EYGCTTYIGNSGDLDEEVASAISDD 147
            YGCTT  GNSGDLDEEVASAISDD
Sbjct: 277 -YGCTTCSGNSGDLDEEVASAISDD 281

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: XP_022145483.1 (aconitate hydratase, cytoplasmic-like isoform X1 [Momordica charantia])

HSP 1 Score: 196.8 bits (499), Expect = 1.3e-46
Identity = 106/146 (72.60%), Postives = 122/146 (83.56%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           +SFA PKESQTKVVEFSFHG  A LRHGDVVIAAI SCTNTSNPSI LGAALVAKKACEL
Sbjct: 188 KSFAIPKESQTKVVEFSFHGKPAALRHGDVVIAAITSCTNTSNPSIMLGAALVAKKACEL 247

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG++VVTKYL+K    L + +   +LL++ +   +KYLD+LGFNTV 
Sbjct: 248 GLEVKPWIKTSLAPGSKVVTKYLQKRCAPLDSPN---ALLMLDENGLQKYLDQLGFNTV- 307

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
           EYGCTT IGNSGDLDEEVASAI++++
Sbjct: 308 EYGCTTCIGNSGDLDEEVASAITEND 329

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: XP_022145484.1 (aconitate hydratase, cytoplasmic-like isoform X2 [Momordica charantia])

HSP 1 Score: 195.3 bits (495), Expect = 3.8e-46
Identity = 104/146 (71.23%), Postives = 115/146 (78.77%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           +SFA PKESQTKVVEFSFHG  A LRHGDVVIAAI SCTNTSNPSI LGAALVAKKACEL
Sbjct: 188 KSFAIPKESQTKVVEFSFHGKPAALRHGDVVIAAITSCTNTSNPSIMLGAALVAKKACEL 247

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG++VVTKYL+KS +                   +KYLD+LGFNTV 
Sbjct: 248 GLEVKPWIKTSLAPGSKVVTKYLQKSGL-------------------QKYLDQLGFNTV- 307

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
           EYGCTT IGNSGDLDEEVASAI++++
Sbjct: 308 EYGCTTCIGNSGDLDEEVASAITEND 313

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: KAA0052937.1 (aconitate hydratase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 194.9 bits (494), Expect = 4.9e-46
Identity = 107/145 (73.79%), Postives = 112/145 (77.24%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PK+SQTKVVEFSF G TAELRHGDVVIAAI SCTNTSNPSI LGAALVAKKACEL
Sbjct: 118 KGFAIPKKSQTKVVEFSFRGRTAELRHGDVVIAAITSCTNTSNPSIMLGAALVAKKACEL 177

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVK WI TGLAPG+ VVTKYLEKS +                   +KYLDKLGFNTVG
Sbjct: 178 CLEVKHWIKTGLAPGSIVVTKYLEKSGL-------------------QKYLDKLGFNTVG 237

Query: 122 EYGCTTYIGNSGDLDEEVASAISDD 147
            YGCTT  GNSGDLDEEVASAISDD
Sbjct: 238 -YGCTTCSGNSGDLDEEVASAISDD 242

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: TYK11394.1 (aconitate hydratase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 194.9 bits (494), Expect = 4.9e-46
Identity = 107/145 (73.79%), Postives = 112/145 (77.24%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PK+SQTKVVEFSF G TAELRHGDVVIAAI SCTNTSNPSI LGAALVAKKACEL
Sbjct: 157 KGFAIPKKSQTKVVEFSFRGRTAELRHGDVVIAAITSCTNTSNPSIMLGAALVAKKACEL 216

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVK WI TGLAPG+ VVTKYLEKS +                   +KYLDKLGFNTVG
Sbjct: 217 CLEVKHWIKTGLAPGSIVVTKYLEKSGL-------------------QKYLDKLGFNTVG 276

Query: 122 EYGCTTYIGNSGDLDEEVASAISDD 147
            YGCTT  GNSGDLDEEVASAISDD
Sbjct: 277 -YGCTTCSGNSGDLDEEVASAISDD 281

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: XP_004287770.1 (PREDICTED: aconitate hydratase 1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 186.8 bits (473), Expect = 1.3e-43
Identity = 98/146 (67.12%), Postives = 113/146 (77.40%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKESQTKVVEFSFHGT A+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKAC+L
Sbjct: 404 KGFAVPKESQTKVVEFSFHGTPAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACDL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYLEKS +                   +KYL++LGFN VG
Sbjct: 464 GLEVKPWIKTSLAPGSGVVTKYLEKSGL-------------------QKYLNQLGFNIVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+D+ VASAI++++
Sbjct: 524 -YGCTTCIGNSGDIDDAVASAITEND 529

[Close]

BLAST of CSPI03G00500 vs. TAIR 10
Match: AT4G35830.1 (aconitase 1 )

HSP 1 Score: 175.6 bits (444), Expect = 2.9e-44
Identity = 93/146 (63.70%), Postives = 110/146 (75.34%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q+K VEF+F+GTTA+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKAC+L
Sbjct: 404 KGFAVPKEAQSKAVEFNFNGTTAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACDL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYL KS +                   +KYL++LGF+ VG
Sbjct: 464 GLEVKPWIKTSLAPGSGVVTKYLAKSGL-------------------QKYLNQLGFSIVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+ E VASAI D++
Sbjct: 524 -YGCTTCIGNSGDIHEAVASAIVDND 529

[Close]

BLAST of CSPI03G00500 vs. TAIR 10
Match: AT4G35830.2 (aconitase 1 )

HSP 1 Score: 175.6 bits (444), Expect = 2.9e-44
Identity = 93/146 (63.70%), Postives = 110/146 (75.34%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q+K VEF+F+GTTA+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKAC+L
Sbjct: 301 KGFAVPKEAQSKAVEFNFNGTTAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACDL 360

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYL KS +                   +KYL++LGF+ VG
Sbjct: 361 GLEVKPWIKTSLAPGSGVVTKYLAKSGL-------------------QKYLNQLGFSIVG 420

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+ E VASAI D++
Sbjct: 421 -YGCTTCIGNSGDIHEAVASAIVDND 426

[Close]

BLAST of CSPI03G00500 vs. TAIR 10
Match: AT2G05710.1 (aconitase 3 )

HSP 1 Score: 159.1 bits (401), Expect = 2.8e-39
Identity = 84/146 (57.53%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q KVV FSF G  AEL+HG VVIAAI SCTNTSNPS+ LGA LVAKKAC+L
Sbjct: 496 KGFAIPKEAQEKVVNFSFDGQPAELKHGSVVIAAITSCTNTSNPSVMLGAGLVAKKACDL 555

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            L+VKPWI T LAPG+ VVTKYL KS +                   ++YL++ GFN VG
Sbjct: 556 GLQVKPWIKTSLAPGSGVVTKYLLKSGL-------------------QEYLNEQGFNIVG 615

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSG+++E V +AI++++
Sbjct: 616 -YGCTTCIGNSGEINESVGAAITEND 621

[Close]

BLAST of CSPI03G00500 vs. TAIR 10
Match: AT4G26970.1 (aconitase 2 )

HSP 1 Score: 153.7 bits (387), Expect = 1.2e-37
Identity = 82/142 (57.75%), Postives = 100/142 (70.42%), Query Frame = 0


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE Q +VV+FS++G  AE++HG VVIAAI SCTNTSNPS+ +GAALVAKKA +L
Sbjct: 501 KGFAVPKEKQEEVVKFSYNGQPAEIKHGSVVIAAITSCTNTSNPSVMIGAALVAKKASDL 560

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            L+VKPW+ T LAPG+RVV KYL++S +                   R+ L K GF  VG
Sbjct: 561 GLKVKPWVKTSLAPGSRVVEKYLDRSGL-------------------RESLTKQGFEIVG 620

Query: 122 EYGCTTYIGNSGDLDEEVASAI 144
            YGCTT IGNSG+LD EVASAI
Sbjct: 621 -YGCTTCIGNSGNLDPEVASAI 622

The following BLAST results are available for this feature:

BLAST of CSPI03G00500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42560	4.1e-43	63.70	Aconitate hydratase 1 OS=Arabidopsis thaliana OX=3702 GN=ACO1 PE=1 SV=2	[more]
Q6YZX6	5.0e-41	60.96	Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica OX=399...	[more]
O04916	1.1e-40	62.33	Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum OX=4113 PE=2 SV...	[more]
P49608	2.5e-40	60.27	Aconitate hydratase, cytoplasmic OS=Cucurbita maxima OX=3661 PE=2 SV=1	[more]
Q42669	1.8e-38	60.96	Aconitate hydratase (Fragment) OS=Cucumis melo var. conomon OX=3657 GN=ACO PE=2 ...	[more]

BLAST of CSPI03G00500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3M2	3.1e-70	91.67	Aconitase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002540 P...	[more]
A0A6J1CW20	6.3e-47	72.60	Aconitate hydratase OS=Momordica charantia OX=3673 GN=LOC111014921 PE=3 SV=1	[more]
A0A6J1CVD7	1.8e-46	71.23	Aconitate hydratase OS=Momordica charantia OX=3673 GN=LOC111014921 PE=3 SV=1	[more]
A0A5A7UEJ6	2.4e-46	73.79	Aconitate hydratase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A5D3CJP4	2.4e-46	73.79	Aconitate hydratase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of CSPI03G00500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145483.1	1.3e-46	72.60	aconitate hydratase, cytoplasmic-like isoform X1 [Momordica charantia]	[more]
XP_022145484.1	3.8e-46	71.23	aconitate hydratase, cytoplasmic-like isoform X2 [Momordica charantia]	[more]
KAA0052937.1	4.9e-46	73.79	aconitate hydratase family protein [Cucumis melo var. makuwa]	[more]
TYK11394.1	4.9e-46	73.79	aconitate hydratase family protein [Cucumis melo var. makuwa]	[more]
XP_004287770.1	1.3e-43	67.12	PREDICTED: aconitate hydratase 1 [Fragaria vesca subsp. vesca]	[more]

BLAST of CSPI03G00500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35830.1	2.9e-44	63.70	aconitase 1	[more]
AT4G35830.2	2.9e-44	63.70	aconitase 1	[more]
AT2G05710.1	2.8e-39	57.53	aconitase 3	[more]
AT4G26970.1	1.2e-37	57.75	aconitase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001030	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain	PFAM	PF00330	Aconitase	coord: 10..88 e-value: 2.8E-17 score: 62.9
IPR015931	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3	GENE3D	3.30.499.10	Aconitase, domain 3	coord: 1..147 e-value: 5.0E-47 score: 161.9
None	No IPR available	PANTHER	PTHR11670:SF62	ACONITATE HYDRATASE 1	coord: 2..146
IPR006249	Aconitase/Iron-responsive element-binding protein 2	PANTHER	PTHR11670	ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER	coord: 2..146
IPR036008	Aconitase, iron-sulfur domain	SUPERFAMILY	53732	Aconitase iron-sulfur domain	coord: 5..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G00500.1	CSPI03G00500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006101	citrate metabolic process
biological_process	GO:0006099	tricarboxylic acid cycle
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0003994	aconitate hydratase activity
molecular_function	GO:0047780	citrate dehydratase activity
molecular_function	GO:0046872	metal ion binding