CSPI03G00490 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G00490
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Aconitate hydratase
Location	Chr3: 368854 .. 369865 (-)
RNA-Seq Expression	CSPI03G00490
Synteny	CSPI03G00490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGTCGTTGGAAACCATGAGGTGATGATCTTTGGTACTTTTGCCAATATAAGACCTGTCAATAAACTATTAGATGGAGAGGTTGGACCTAAAACTATTCACTTTCCCACTGGAGAGAAACTTCCCATCTTTGATGTTGCCATGGTACGGATATCTTTCTTTCGTCTTCAGCAAGAAATTTGTTATTTATTTCCTTTTATCCTCTTTCAGTCAATTAAGTTGATGGGTATATTTACTCTTGACTCAATACTAACTTGACAGACATACAAGAGTGAGGGTCATGATACCATTGTTCTTGCGGGATCGATTATGGAAGTGGGAGTTCCCAGGATTGGGCTGCTAAAGGACCAATGCTTCTGGTAAGTTTCCAACAGCGTCTGTATTCAATTGAGAATTTGTATTTGCCTTTTCTAGTGATAGCTTAAGTATTGTATTCGGTGACAGGGAGTAAAAGCAGTGATTGCTAAAAGCTTTGAGCATACTCATCGCAGTAATTTGGTGGGAATGGGAATAATTACTCTCTGCTTTAAGCAAGGAGAAGATACTGAATCTCTTGGTCTGTCGGGTCGCAAATGCTGTAACATTGATCTTCCAAACAGCATCAAGGATCTCAAACCATTTCCAGATATAACTGGTACGACAGAAGAAGCTGAAACTAGGAAGTCCTTTCAATGCACCCTAAGAGTTAACACAGAGGTATATCAAATCAGCTAGCTTATATCGTCAAAATTTTGGCCTTGTTAAGATTAGGAAAGTTCAGGCATTGGTTAAAAATGGGTGCAGGTTGAACTCACTTATTTTGATCATGGAGGAATTCTACACTACGTGATAAGAAGTTTGATCAACACACAAGACTGAGTCATTAACAAGGCCAGCTTGGATGGTCGAGGCATTTCTCTTCTTCATGGACATAACAAAAACCAGATTCTCCACGTTTCTACACAAGACTTACGAGTATCCAGATCCAGGATATGAAGCAAATTTCCATTTTGCTTTCCAGCTTATGTGA

mRNA sequence

ATGGCAGTCGTTGGAAACCATGAGGTGATGATCTTTGGTACTTTTGCCAATATAAGACCTGTCAATAAACTATTAGATGGAGAGGTTGGACCTAAAACTATTCACTTTCCCACTGGAGAGAAACTTCCCATCTTTGATGTTGCCATGACATACAAGAGTGAGGGTCATGATACCATTGTTCTTGCGGGATCGATTATGGAAGTGGGAGTTCCCAGGATTGGGCTGCTAAAGGACCAATGCTTCTGTAATTTGGTGGGAATGGGAATAATTACTCTCTGCTTTAAGCAAGGAGAAGATACTGAATCTCTTGGTCTGTCGGGTCGCAAATGCTGTAACATTGATCTTCCAAACAGCATCAAGGATCTCAAACCATTTCCAGATATAACTGGTACGACAGAAGAAGCTGAAACTAGGAAGTCCTTTCAATGCACCCTAAGAGTTAACACAGAGACTGAGTCATTAACAAGGCCAGCTTGGATGGTCGAGGCATTTCTCTTCTTCATGGACATAACAAAAACCAGATTCTCCACGTTTCTACACAAGACTTACGAGTATCCAGATCCAGGATATGAAGCAAATTTCCATTTTGCTTTCCAGCTTATGTGA

Coding sequence (CDS)

ATGGCAGTCGTTGGAAACCATGAGGTGATGATCTTTGGTACTTTTGCCAATATAAGACCTGTCAATAAACTATTAGATGGAGAGGTTGGACCTAAAACTATTCACTTTCCCACTGGAGAGAAACTTCCCATCTTTGATGTTGCCATGACATACAAGAGTGAGGGTCATGATACCATTGTTCTTGCGGGATCGATTATGGAAGTGGGAGTTCCCAGGATTGGGCTGCTAAAGGACCAATGCTTCTGTAATTTGGTGGGAATGGGAATAATTACTCTCTGCTTTAAGCAAGGAGAAGATACTGAATCTCTTGGTCTGTCGGGTCGCAAATGCTGTAACATTGATCTTCCAAACAGCATCAAGGATCTCAAACCATTTCCAGATATAACTGGTACGACAGAAGAAGCTGAAACTAGGAAGTCCTTTCAATGCACCCTAAGAGTTAACACAGAGACTGAGTCATTAACAAGGCCAGCTTGGATGGTCGAGGCATTTCTCTTCTTCATGGACATAACAAAAACCAGATTCTCCACGTTTCTACACAAGACTTACGAGTATCCAGATCCAGGATATGAAGCAAATTTCCATTTTGCTTTCCAGCTTATGTGA

Protein sequence

MAVVGNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGSIMEVGVPRIGLLKDQCFCNLVGMGIITLCFKQGEDTESLGLSGRKCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETESLTRPAWMVEAFLFFMDITKTRFSTFLHKTYEYPDPGYEANFHFAFQLM*

Homology

[Close]

BLAST of CSPI03G00490 vs. ExPASy Swiss-Prot
Match: Q6YZX6 (Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0191100 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-39
Identity = 89/164 (54.27%), Postives = 104/164 (63.41%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNK L+GEVGPKT+H PTGEKL +FD A+ YKSEGHDTIVLAG+
Sbjct: 718 GNDEVMARGTFANIRIVNKFLNGEVGPKTVHVPTGEKLYVFDAALKYKSEGHDTIVLAGA 777

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED +SLGL+G 
Sbjct: 778 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGH 837

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLP ++ +++P  DIT TT+     KSF CTLR +TE E
Sbjct: 838 ERYTIDLPTNVSEIRPGQDITVTTDNG---KSFTCTLRFDTEVE 878

[Close]

BLAST of CSPI03G00490 vs. ExPASy Swiss-Prot
Match: Q42560 (Aconitate hydratase 1 OS=Arabidopsis thaliana OX=3702 GN=ACO1 PE=1 SV=2)

HSP 1 Score: 158.7 bits (400), Expect = 7.0e-38
Identity = 84/164 (51.22%), Postives = 102/164 (62.20%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNK L GEVGPKT+H PTGEKL +FD AM Y++EG DTI+LAG+
Sbjct: 718 GNDEIMARGTFANIRIVNKHLKGEVGPKTVHIPTGEKLSVFDAAMKYRNEGRDTIILAGA 777

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G+
Sbjct: 778 EYGSGSSRDWAAKGPMLLGVKAVISKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGQ 837

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   I+LPN++ ++KP  D+T  T      KSF CTLR +TE E
Sbjct: 838 ELYTIELPNNVSEIKPGQDVTVVTNNG---KSFTCTLRFDTEVE 878

[Close]

BLAST of CSPI03G00490 vs. ExPASy Swiss-Prot
Match: P49608 (Aconitate hydratase, cytoplasmic OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 9.1e-38
Identity = 87/164 (53.05%), Postives = 101/164 (61.59%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNKLLDGEVGPKT+H PTGEKL +F+ A  YKS G DTIVLAG+
Sbjct: 718 GNDEVMARGTFANIRLVNKLLDGEVGPKTVHVPTGEKLSVFEAAEKYKSAGQDTIVLAGA 777

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED +SLGL+G 
Sbjct: 778 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKSGEDADSLGLTGH 837

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLP+ I  ++P  D+T TT+     KSF CT+R +TE E
Sbjct: 838 ERYTIDLPDDISKIRPGQDVTVTTDSG---KSFTCTVRFDTEVE 878

[Close]

BLAST of CSPI03G00490 vs. ExPASy Swiss-Prot
Match: Q9SIB9 (Aconitate hydratase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ACO3 PE=1 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 7.7e-37
Identity = 82/164 (50.00%), Postives = 101/164 (61.59%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNKL++GEVGPKT+H P+GEKL +FD AM YKS G DTI+LAG+
Sbjct: 810 GNDEIMARGTFANIRIVNKLMNGEVGPKTVHIPSGEKLSVFDAAMRYKSSGEDTIILAGA 869

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED ++LGL+G 
Sbjct: 870 EYGSGSSRDWAAKGPMLQGVKAVIAKSFERIHRSNLVGMGIIPLCFKSGEDADTLGLTGH 929

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   I LP  I +++P  D+T TT+     KSF CT+R +TE E
Sbjct: 930 ERYTIHLPTDISEIRPGQDVTVTTDNG---KSFTCTVRFDTEVE 970

[Close]

BLAST of CSPI03G00490 vs. ExPASy Swiss-Prot
Match: O04916 (Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 8.6e-36
Identity = 81/164 (49.39%), Postives = 102/164 (62.20%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNKLL+GEVGPKT+H P+GEKL +FD AM YKS G  TI+LAG+
Sbjct: 436 GNDEIMARGTFANIRLVNKLLNGEVGPKTVHVPSGEKLSVFDAAMKYKSAGQSTIILAGA 495

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGI+ LCFK GED ++LGL+G+
Sbjct: 496 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIVPLCFKAGEDADTLGLTGQ 555

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLP +I +++P  D+T  T   +T KSF C +R +TE E
Sbjct: 556 ERYTIDLPENISEIRPGQDVTVQT---DTGKSFTCIVRFDTEVE 596

[Close]

BLAST of CSPI03G00490 vs. ExPASy TrEMBL
Match: A0A0A0L1K8 (Aconitase_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002530 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 9.1e-49
Identity = 106/154 (68.83%), Postives = 112/154 (72.73%), Query Frame = 0


Query: 2   AVVGNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVL 61
           +VVGNHEVMIFGTFANIRPVNKLLDGEVGPKTI FP    LP     M       D    
Sbjct: 57  SVVGNHEVMIFGTFANIRPVNKLLDGEVGPKTITFPLERNLPSL---MLPCGSSQDWAAK 116

Query: 62  AGSIMEVGVPRIGLLKDQCFCNLVGMGIITLCFKQGEDTESLGLSGRKCCNIDLPNSIKD 121
              ++ V        +     NLVGMGIITLCFKQGEDTESLGLSG KCCNIDLPNSIKD
Sbjct: 117 GPMLLGVKAVIAKSFEHTHRSNLVGMGIITLCFKQGEDTESLGLSGHKCCNIDLPNSIKD 176

Query: 122 LKPFPDITGTTEEAETRKSFQCTLRVNTETESLT 156
           LKPFPDITGTTEEAETRKSFQCTLRVNTE   ++
Sbjct: 177 LKPFPDITGTTEEAETRKSFQCTLRVNTEVYQIS 207

[Close]

BLAST of CSPI03G00490 vs. ExPASy TrEMBL
Match: A0A6J1CVD7 (Aconitate hydratase OS=Momordica charantia OX=3673 GN=LOC111014921 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 3.7e-42
Identity = 96/164 (58.54%), Postives = 112/164 (68.29%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GNHEVMI GTFANIR VNKLLDGEVGP+TI FPTG+KL +FD A+ Y+SEGHDTI+LAG+
Sbjct: 502 GNHEVMIRGTFANIRLVNKLLDGEVGPRTIQFPTGDKLSVFDAAVRYRSEGHDTIILAGA 561

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII +CFKQGED ESLGL+GR
Sbjct: 562 EYGSGSSRDWAAKGPMLLGVKAVIAKSFEHTHRSNLVGMGIIPICFKQGEDAESLGLTGR 621

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +  ++ +PN+IKDL+P  DIT     AE RKSFQCTLR +TE E
Sbjct: 622 ERYSVKIPNNIKDLRPCQDIT-VMAAAENRKSFQCTLRFDTEVE 664

[Close]

BLAST of CSPI03G00490 vs. ExPASy TrEMBL
Match: A0A6J1CW20 (Aconitate hydratase OS=Momordica charantia OX=3673 GN=LOC111014921 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 3.7e-42
Identity = 96/164 (58.54%), Postives = 112/164 (68.29%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GNHEVMI GTFANIR VNKLLDGEVGP+TI FPTG+KL +FD A+ Y+SEGHDTI+LAG+
Sbjct: 518 GNHEVMIRGTFANIRLVNKLLDGEVGPRTIQFPTGDKLSVFDAAVRYRSEGHDTIILAGA 577

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII +CFKQGED ESLGL+GR
Sbjct: 578 EYGSGSSRDWAAKGPMLLGVKAVIAKSFEHTHRSNLVGMGIIPICFKQGEDAESLGLTGR 637

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +  ++ +PN+IKDL+P  DIT     AE RKSFQCTLR +TE E
Sbjct: 638 ERYSVKIPNNIKDLRPCQDIT-VMAAAENRKSFQCTLRFDTEVE 680

[Close]

BLAST of CSPI03G00490 vs. ExPASy TrEMBL
Match: A0A059AHS0 (Aconitate hydratase OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_J02488 PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.9e-38
Identity = 89/164 (54.27%), Postives = 106/164 (64.63%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN+EVM+ GTFAN+R VNKLL+GEVGP+TIH P+GEK+ +FD AM YK+EG DTIVLAGS
Sbjct: 746 GNYEVMVRGTFANVRIVNKLLEGEVGPRTIHIPSGEKMHVFDAAMRYKNEGQDTIVLAGS 805

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
              VG  R G  K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 806 EYGVGSSRDGAAKGPMLVGVQAVIAKSFEQIHRSNLVGMGIIPLCFKPGEDAETLGLNGH 865

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN + +LKP  D+   T+     KSF CTLR +TE E
Sbjct: 866 ERYTIDLPNKVSELKPRQDVKVVTDNG---KSFACTLRFDTEVE 906

[Close]

BLAST of CSPI03G00490 vs. ExPASy TrEMBL
Match: A0A445BWT0 (Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_A08g039624 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 2.5e-38
Identity = 92/164 (56.10%), Postives = 105/164 (64.02%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNKLL+GEVGPKT+H PTGEKL +FD AM YK+EGHDTIVLAG+
Sbjct: 821 GNDEVMARGTFANIRIVNKLLNGEVGPKTVHIPTGEKLYVFDAAMRYKNEGHDTIVLAGA 880

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 881 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGH 940

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN I D++P  D+T TT    T KSF CT+R +TE E
Sbjct: 941 ERYTIDLPNKISDIRPGQDVTVTT---NTGKSFTCTVRFDTEVE 981

[Close]

BLAST of CSPI03G00490 vs. NCBI nr
Match: XP_022145483.1 (aconitate hydratase, cytoplasmic-like isoform X1 [Momordica charantia])

HSP 1 Score: 181.4 bits (459), Expect = 7.7e-42
Identity = 96/164 (58.54%), Postives = 112/164 (68.29%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GNHEVMI GTFANIR VNKLLDGEVGP+TI FPTG+KL +FD A+ Y+SEGHDTI+LAG+
Sbjct: 518 GNHEVMIRGTFANIRLVNKLLDGEVGPRTIQFPTGDKLSVFDAAVRYRSEGHDTIILAGA 577

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII +CFKQGED ESLGL+GR
Sbjct: 578 EYGSGSSRDWAAKGPMLLGVKAVIAKSFEHTHRSNLVGMGIIPICFKQGEDAESLGLTGR 637

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +  ++ +PN+IKDL+P  DIT     AE RKSFQCTLR +TE E
Sbjct: 638 ERYSVKIPNNIKDLRPCQDIT-VMAAAENRKSFQCTLRFDTEVE 680

[Close]

BLAST of CSPI03G00490 vs. NCBI nr
Match: XP_022145484.1 (aconitate hydratase, cytoplasmic-like isoform X2 [Momordica charantia])

HSP 1 Score: 181.4 bits (459), Expect = 7.7e-42
Identity = 96/164 (58.54%), Postives = 112/164 (68.29%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GNHEVMI GTFANIR VNKLLDGEVGP+TI FPTG+KL +FD A+ Y+SEGHDTI+LAG+
Sbjct: 502 GNHEVMIRGTFANIRLVNKLLDGEVGPRTIQFPTGDKLSVFDAAVRYRSEGHDTIILAGA 561

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII +CFKQGED ESLGL+GR
Sbjct: 562 EYGSGSSRDWAAKGPMLLGVKAVIAKSFEHTHRSNLVGMGIIPICFKQGEDAESLGLTGR 621

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +  ++ +PN+IKDL+P  DIT     AE RKSFQCTLR +TE E
Sbjct: 622 ERYSVKIPNNIKDLRPCQDIT-VMAAAENRKSFQCTLRFDTEVE 664

[Close]

BLAST of CSPI03G00490 vs. NCBI nr
Match: KCW53221.1 (hypothetical protein EUGRSUZ_J02488 [Eucalyptus grandis])

HSP 1 Score: 169.1 bits (427), Expect = 3.9e-38
Identity = 89/164 (54.27%), Postives = 106/164 (64.63%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN+EVM+ GTFAN+R VNKLL+GEVGP+TIH P+GEK+ +FD AM YK+EG DTIVLAGS
Sbjct: 746 GNYEVMVRGTFANVRIVNKLLEGEVGPRTIHIPSGEKMHVFDAAMRYKNEGQDTIVLAGS 805

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
              VG  R G  K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 806 EYGVGSSRDGAAKGPMLVGVQAVIAKSFEQIHRSNLVGMGIIPLCFKPGEDAETLGLNGH 865

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN + +LKP  D+   T+     KSF CTLR +TE E
Sbjct: 866 ERYTIDLPNKVSELKPRQDVKVVTDNG---KSFACTLRFDTEVE 906

[Close]

BLAST of CSPI03G00490 vs. NCBI nr
Match: RYQ97674.1 (hypothetical protein Ahy_B08g093753 isoform A [Arachis hypogaea])

HSP 1 Score: 168.7 bits (426), Expect = 5.2e-38
Identity = 92/164 (56.10%), Postives = 105/164 (64.02%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNKLL+GEVGPKT+H PTGEKL +FD AM YK+EGHDTIVLAG+
Sbjct: 821 GNDEVMARGTFANIRIVNKLLNGEVGPKTVHIPTGEKLYVFDAAMRYKNEGHDTIVLAGA 880

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 881 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGH 940

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN I D++P  D+T TT    T KSF CT+R +TE E
Sbjct: 941 ERYTIDLPNKISDIRPGQDVTVTT---NTGKSFTCTVRFDTEVE 981

[Close]

BLAST of CSPI03G00490 vs. NCBI nr
Match: RYR43194.1 (hypothetical protein Ahy_A08g039624 isoform A [Arachis hypogaea])

HSP 1 Score: 168.7 bits (426), Expect = 5.2e-38
Identity = 92/164 (56.10%), Postives = 105/164 (64.02%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNKLL+GEVGPKT+H PTGEKL +FD AM YK+EGHDTIVLAG+
Sbjct: 821 GNDEVMARGTFANIRIVNKLLNGEVGPKTVHIPTGEKLYVFDAAMRYKNEGHDTIVLAGA 880

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 881 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGH 940

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN I D++P  D+T TT    T KSF CT+R +TE E
Sbjct: 941 ERYTIDLPNKISDIRPGQDVTVTT---NTGKSFTCTVRFDTEVE 981

[Close]

BLAST of CSPI03G00490 vs. TAIR 10
Match: AT4G35830.1 (aconitase 1 )

HSP 1 Score: 158.7 bits (400), Expect = 5.0e-39
Identity = 84/164 (51.22%), Postives = 102/164 (62.20%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNK L GEVGPKT+H PTGEKL +FD AM Y++EG DTI+LAG+
Sbjct: 718 GNDEIMARGTFANIRIVNKHLKGEVGPKTVHIPTGEKLSVFDAAMKYRNEGRDTIILAGA 777

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G+
Sbjct: 778 EYGSGSSRDWAAKGPMLLGVKAVISKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGQ 837

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   I+LPN++ ++KP  D+T  T      KSF CTLR +TE E
Sbjct: 838 ELYTIELPNNVSEIKPGQDVTVVTNNG---KSFTCTLRFDTEVE 878

[Close]

BLAST of CSPI03G00490 vs. TAIR 10
Match: AT4G35830.2 (aconitase 1 )

HSP 1 Score: 158.7 bits (400), Expect = 5.0e-39
Identity = 84/164 (51.22%), Postives = 102/164 (62.20%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNK L GEVGPKT+H PTGEKL +FD AM Y++EG DTI+LAG+
Sbjct: 615 GNDEIMARGTFANIRIVNKHLKGEVGPKTVHIPTGEKLSVFDAAMKYRNEGRDTIILAGA 674

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G+
Sbjct: 675 EYGSGSSRDWAAKGPMLLGVKAVISKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGQ 734

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   I+LPN++ ++KP  D+T  T      KSF CTLR +TE E
Sbjct: 735 ELYTIELPNNVSEIKPGQDVTVVTNNG---KSFTCTLRFDTEVE 775

[Close]

BLAST of CSPI03G00490 vs. TAIR 10
Match: AT2G05710.1 (aconitase 3 )

HSP 1 Score: 155.2 bits (391), Expect = 5.5e-38
Identity = 82/164 (50.00%), Postives = 101/164 (61.59%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNKL++GEVGPKT+H P+GEKL +FD AM YKS G DTI+LAG+
Sbjct: 810 GNDEIMARGTFANIRIVNKLMNGEVGPKTVHIPSGEKLSVFDAAMRYKSSGEDTIILAGA 869

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED ++LGL+G 
Sbjct: 870 EYGSGSSRDWAAKGPMLQGVKAVIAKSFERIHRSNLVGMGIIPLCFKSGEDADTLGLTGH 929

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   I LP  I +++P  D+T TT+     KSF CT+R +TE E
Sbjct: 930 ERYTIHLPTDISEIRPGQDVTVTTDNG---KSFTCTVRFDTEVE 970

[Close]

BLAST of CSPI03G00490 vs. TAIR 10
Match: AT4G26970.1 (aconitase 2 )

HSP 1 Score: 149.1 bits (375), Expect = 3.9e-36
Identity = 81/164 (49.39%), Postives = 96/164 (58.54%), Query Frame = 0


Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNKLL GEVGP T+H PTGEKL +FD A  YK+   DTI+LAG+
Sbjct: 815 GNDEVMARGTFANIRIVNKLLKGEVGPNTVHIPTGEKLSVFDAASKYKTAEQDTIILAGA 874

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NL GMGII LCFK GED E+LGL+G 
Sbjct: 875 EYGSGSSRDWAAKGPLLLGVKAVIAKSFERIHRSNLAGMGIIPLCFKAGEDAETLGLTGH 934

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   + LP  + D++P  D+T TT+     KSF CTLR +TE E
Sbjct: 935 ERYTVHLPTKVSDIRPGQDVTVTTDSG---KSFVCTLRFDTEVE 975

[Close]

BLAST of CSPI03G00490 vs. TAIR 10
Match: AT5G54950.1 (Aconitase family protein )

HSP 1 Score: 62.4 bits (150), Expect = 4.8e-10
Identity = 31/59 (52.54%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 92  LCFKQGEDTESLGLSGRKCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTE 151
           + FK GED E+LGL+G +   I LP++I ++KP  DIT TT   +T KSF CTLR++TE
Sbjct: 1   MAFKSGEDAETLGLTGHELYTIHLPSNINEIKPGQDITVTT---DTAKSFVCTLRLDTE 56

The following BLAST results are available for this feature:

BLAST of CSPI03G00490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6YZX6	1.3e-39	54.27	Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica OX=399...	[more]
Q42560	7.0e-38	51.22	Aconitate hydratase 1 OS=Arabidopsis thaliana OX=3702 GN=ACO1 PE=1 SV=2	[more]
P49608	9.1e-38	53.05	Aconitate hydratase, cytoplasmic OS=Cucurbita maxima OX=3661 PE=2 SV=1	[more]
Q9SIB9	7.7e-37	50.00	Aconitate hydratase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ACO3 PE=...	[more]
O04916	8.6e-36	49.39	Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum OX=4113 PE=2 SV...	[more]

BLAST of CSPI03G00490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1K8	9.1e-49	68.83	Aconitase_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002530...	[more]
A0A6J1CVD7	3.7e-42	58.54	Aconitate hydratase OS=Momordica charantia OX=3673 GN=LOC111014921 PE=3 SV=1	[more]
A0A6J1CW20	3.7e-42	58.54	Aconitate hydratase OS=Momordica charantia OX=3673 GN=LOC111014921 PE=3 SV=1	[more]
A0A059AHS0	1.9e-38	54.27	Aconitate hydratase OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_J02488 PE=3 SV=1	[more]
A0A445BWT0	2.5e-38	56.10	Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_A08g039624 PE=3 SV=1	[more]

BLAST of CSPI03G00490 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145483.1	7.7e-42	58.54	aconitate hydratase, cytoplasmic-like isoform X1 [Momordica charantia]	[more]
XP_022145484.1	7.7e-42	58.54	aconitate hydratase, cytoplasmic-like isoform X2 [Momordica charantia]	[more]
KCW53221.1	3.9e-38	54.27	hypothetical protein EUGRSUZ_J02488 [Eucalyptus grandis]	[more]
RYQ97674.1	5.2e-38	56.10	hypothetical protein Ahy_B08g093753 isoform A [Arachis hypogaea]	[more]
RYR43194.1	5.2e-38	56.10	hypothetical protein Ahy_A08g039624 isoform A [Arachis hypogaea]	[more]

BLAST of CSPI03G00490 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35830.1	5.0e-39	51.22	aconitase 1	[more]
AT4G35830.2	5.0e-39	51.22	aconitase 1	[more]
AT2G05710.1	5.5e-38	50.00	aconitase 3	[more]
AT4G26970.1	3.9e-36	49.39	aconitase 2	[more]
AT5G54950.1	4.8e-10	52.54	Aconitase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000573	Aconitase A/isopropylmalate dehydratase small subunit, swivel domain	PFAM	PF00694	Aconitase_C	coord: 4..63 e-value: 5.9E-7 score: 29.9
IPR015928	Aconitase/3-isopropylmalate dehydratase, swivel	GENE3D	3.20.19.10	Aconitase, domain 4	coord: 76..156 e-value: 2.5E-9 score: 38.7 coord: 1..66 e-value: 2.6E-16 score: 61.6
None	No IPR available	PANTHER	PTHR11670:SF59	ACONITATE HYDRATASE	coord: 5..152
None	No IPR available	SUPERFAMILY	52016	LeuD/IlvD-like	coord: 4..127
IPR006249	Aconitase/Iron-responsive element-binding protein 2	PANTHER	PTHR11670	ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER	coord: 5..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G00490.1	CSPI03G00490.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043436	oxoacid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016836	hydro-lyase activity
molecular_function	GO:0051536	iron-sulfur cluster binding